Publications

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relative toxicity of various insecticides to the buffalo fly, lyperosia exigua (de meij, 1903). 1979489103
the survival and fecundity of buffalo flies after treatment of cattle with three anthelmintics.two anthelmintics with known insecticidal action (ivermectin and closantel) and one with no recorded effect on insects (levamisole) were tested to evaluate their effects on buffalo fly (haematobia irritans exigua). blood from animals given closantel or levamisole had no significant effect on mortality of buffalo flies in an in-vitro assay. in contrast, blood from animals given ivermectin showed a dose-dependent effect on the mortality of buffalo flies. at 24 h after one injection of the recommen ...19921288475
enzymes in saliva from four parasitic arthropods.enzyme assays and sds polyacrylamide gel electrophoresis were carried out on saliva and in some cases homogenates of salivary gland and gut from four parasitic arthropods (the cattle tick, boophilus microplus (canestrini); the mosquito, aedes aegypti (l.); non-parasitic adult and parasitic larval blowfly of sheep, lucilia cuprina (wiedemann); the buffalo fly, haematobia irritans exigua de meijere). saliva from all species showed large differences in the number and molecular weight of components, ...19921330086
vaccine r & d at csiro's division of tropical animal production.the extensive research on animal vaccines being undertaken by the csiro division of tropical animal production in brisbane is summarised in this paper. much of the research is being developed in conjunction with commercial partners. vaccines are being developed for the cattle tick, babesia, anaplasma, sheep blowfly, buffalo fly and bovine ephemeral fever.19911367735
acquired immune response of cattle exposed to buffalo fly (haematobia irritans exigua).naturally acquired immunity to buffalo fly (haematobia irritans exigua) infestation was examined in cattle. animals exposed to flies had serum antibodies to buffalo fly antigens at levels that correlated with the intensity of exposure. two weeks of intense exposure to buffalo fly induced an increase in peripheral blood eosinophil numbers and a concomitant rise in serum antibody levels in exposed animals. antigens specific for antibody induced by natural exposure were identified using antisera fr ...19921496794
the relationships among ecto- and endoparasite levels, class i antigens of the bovine major histocompatibility system, immunoglobulin e levels and weight gain.natural infestations of the cattle tick boophilus microplus, levels of the buffalo fly haematobia irritants exigua and faecal nematode egg concentrations (bunostomum phlebotomum, cooperia spp., haemonchus placei, oesophagostomum radiatum and trichostrongylus axei) were assessed in 221 belmont red calves during the post-weaning period, when the animals were between 9 and 18 months of age. in addition, the 98 males of this group were challenged with b. microplus larvae on two separate occasions. t ...19902316176
the distribution and prevalence of stephanofilariasis in cattle in queensland.a total of 10,543 cattle from 1,386 farms throughout queensland was examined at abattoirs and the presence of stephanofilarial lesions was related to property of origin, sex, breed, and age of the slaughtered cattle and season of slaughter. the mean prevalence was 38% and within the infected area this varied from less than 5% in south east queensland to 95% on cape york peninsula. the prevalence of lesions was higher in bulls than in steers [corrected] and was least in cows [corrected]. bos indi ...19863741275
the southward spread of buffalo fly (haematobia irritans exigua) in eastern australia and its survival through a severe winter.the southward spread of buffalo fly (haematobia irritans exigua) in coastal eastern australia from bororen, queensland in 1974 to coffs harbour, new south wales in 1982 is documented. surveys after a cold, dry winter in 1982 recorded overwintering sites and subsequent spread of flies in southern queensland and northern new south wales. buffalo fly overwintered at several places in the maryborough-bauple districts and further north. overwintering also occurred in the currumbin valley-mudgeeraba a ...19853834900
methoxychlor residues in the meat and milk of cattle following limited applications of methoxychlor for the control of buffalo fly (haematobia exigua). 19685695696
colonisation of an island by the buffalo fly, haematobia exigua. 19695816699
cephalic neuroendocrine system of the buffalo-fly, lyperosia exigua (de meijere) (diptera: muscidae).in the adult buffalo-fly, l. exigua three groups of neurosecretory cells are present in each half of the brain; medial and lateral groups in the protocerebrum and ventral group in the tritocerebral region. the cerebral neurosecretory cells are classified as a and b cells. both the cells are present in the medial, lateral and ventral groups. histochemically, the neurosecretory material of these cells is composed of neutral mucopolysaccharides and proteins. the a cells are rich in cystine or cyste ...19806108976
use of insecticide-impregnated ear-tags to eradicate the buffalo fly from cattle. 19836667221
changes in the neuroendocrine system during post-embryonic development in the buffalo-fly, lyperosia exigua (de meijere) (diptera: muscidae).the neurosecretory cells of the pars intercerebralis and ring gland are examined in histological sections of larva, pupa and adult of buffalo-fly, lyperosia exigua fixed at various intervals during the rpost-embryonic development. three paired medial groups of the neurosecretory cells are observed in the larval brain. these groups show their displacement during the larval-pupal-adult moult. the medial neurosecretory a cells exhibit secretory activity by undergoing cyclic changes of synthesis and ...19827096991
cyhalothrin--a novel acaricidal and insecticidal synthetic pyrethroid for the control of the cattle tick (boophilus microplus) and the buffalo fly (haematobia irritans exigua).cyhalothrin, a novel synthetic pyrethroid, was evaluated for control of the major resistant strains of the cattle tick (boophilus microplus) and for control of the buffalo fly (haematobia irritans exigua) on cattle. in regulated treatment trials with 0.007% cyhalothrin, greater than 99% control of the biarra, mackay, mt alford, ddt resistant and ulam cattle tick strains was obtained. protective-period trials were conducted in which animals which had been sprayed with 0.007% cyhalothrin then rece ...19827165598
correlative changes in the neurosecretory cells, corpus cardiacum and corpus allatum during egg maturation in the buffalo-fly, lyperosia exigua (de meijere) (diptera: muscidae).the neurosecretory cells of pars intercerebralis, the corpus cardiacum and the corpus allatum are examined in histological sections of lyperosia exigua females, fixed at daily interval during first cycle of egg maturation. the median neurosecretory a cells exhibit secretory activity by undergoing cyclical changes of synthesis and release with the advancing age and egg maturation. similarly, a single corpus allatum also undergoes cyclical changes in volume and histological appearance in accordanc ...19827168247
diseases of cattle in the solomon islands.between 1967 and 1977 a study was made of diseases of cattle in the solomon islands. tuberculosis was found in only 3 herds and was eradicated by 1975. brucellosis serology revealed very few reactors and by 1977 the herds involved were considered free of the disease. significant serological reactions were found to leptospira interrogans serovars pomona, hardjo, autumnalis and jez-bratislava. there was evidence that infectious bovine rhinotracheitis and mucosal disease were present. seventeen par ...19807247885
a survey of cattle producers in the boophilus microplus endemic area of queensland to determine attitudes to the control of and vaccination against tick fever.a survey by mail was used to determine the views of beef producers in the boophilus microplus endemic area of queensland on the control of and vaccination against tick fever. data from 448 questionnaires were analysed, representing 2.7% of beef producers in the survey area. producers considered buffalo fly (haematobia irritans exigua) infestation as the most important problem whereas tick fever ranked sixth overall. private veterinarians were regarded as the most important source of information ...19957611988
isolation and characterization of a trypsin-like enzyme from the buffalo fly, haematobia irritans exigua.the incorporation of soybean trypsin inhibitor (sbti) into the diet of the buffalo fly, haematobia irritans exigua (de meijere), results in increased mortality and reduced fecundity. a trypsin-like enzyme which binds to sbti was isolated by affinity chromatography on a sepharose-sbti column followed by ion-exchange chromatography. the enzyme was inhibited by benzamidine, phenylmethylsulfonyl fluoride, ovomucoid, leupeptin and alpha-2 macroglobulin. the enzyme was not inhibited by edta or p-chlor ...19957787218
an attempt to transmit anaplasma marginale by buffalo flies (haematobia irritans exigua). 19948048908
a family of serine protease genes expressed in adult buffalo fly (haematobia irritans exigua).gene fragments encoding serine proteases expressed in adult buffalo fly (haematobia irritans exigua) were amplified from cdna using generic oligonucleotide pcr primers, based on conserved residues surrounding the active-site his and ser amino acids found in all serine proteases. the pcr product consisted of a broad band extending from about 450 bp to 520 bp, which suggested that the pcr product actually consisted of numerous dna fragments of slightly variable sizes. seventeen independent clones ...19938341258
efficacy of ivermectin as a pour-on formulation for control of buffalo fly. 19938343089
the efficacy of ivermectin topically applied to cattle for control of adult buffalo flies (haematobia irritans exigua). 19938343097
cloning and characterisation of angiotensin-converting enzyme from the dipteran species, haematobia irritans exigua, and its expression in the maturing male reproductive system.the angiotensin-converting enzymes (ace) are involved in the regulation of the specific maturation or degradation of a number of mammalian bioactive peptides. a carboxydipeptidase similar to mammalian ace has now been identified in the adult stage of the haematophagous fly, haematobia irritans exigua (buffalo fly), a close relative of the horn fly of north america. the enzyme was purified by lectin-affinity chromatography and ion-exchange chromatography and migrated as a doublet of 70 kda upon r ...19968647080
the efficacy of diazinon impregnated ear tags against buffalo fly and resulting weight gains and diazinon residues in meat and milk.ear tags impregnated with 20% diazinon were evaluated for their efficacy against the buffalo fly (haematobia irritans exigua) on beef cattle in southern queensland. buffalo fly numbers and weight changes were recorded and diazinon residues in tissues of beef cattle and milk from lactating dairy cattle were assayed at different time intervals after tagging. in 2-efficacy trials conducted over 19 and 20 weeks, the mean numbers of buffalo fly on cattle each fitted with ear tags were 1 to 9 and 0 to ...19968660195
expression of angiotensin-converting enzyme-related carboxydipeptidases in the larvae of four species of fly.hieace, a soluble 70 kda protein related to the angiotensin-converting enzyme (ace) has recently been identified, characterized and cloned from the adult buffalo fly (haematobia irritans exigua). hieace is enzymatically similar to the mammalian aces and its predicted amino acid sequence has 42% identity with the mammalian testicular aces. in adult h.i. exigua, hieace expression is restricted to the compound ganglion and posterior midgut, and the maturing male reproductive system. western blot an ...19979219369
immunocytochemical distribution of angiotensin i-converting enzyme-like immunoreactivity in the brain and testis of insects.angiotensin converting enzyme (ace) is zn2+ metallopeptidase which plays an important role in blood pressure homeostasis in mammals and other vertebrates. homologues of ace involved in the biosynthesis of mammalian peptide hormones have also been identified in the insects, musca domestica, drosophila melanogaster and haematobia irritans exigua. in the pursuit of the biological role of insect ace, this work focused on the tissue and cellular distribution of ace in several insect species. the loca ...19989518620
efficacy of zeta-cypermethrin as pour-on or spray formulations for the control of buffalo fly (haematobia irritans exigua) in cattle.to determine the efficacy of zeta-cypermethrin in controlling buffalo fly (haematobia irritans exigua).19989791712
molecular cloning and expression in escherichia coli of an aquaporin-like gene from adult buffalo fly (haematobia irritans exigua).a gene fragment encoding a putative member of the aquaporin gene family was amplified using cdna prepared from unfed adult buffalo fly poly(a)+ rna and degenerate pcr primers designed from highly conserved regions of amino acids found in all members of the aquaporin gene family. this pcr product was labelled with digoxigenin-dutp and used as a probe to screen a lambdagt-11 cdna library constructed from unfed adult buffalo fly. one positively hybridizing clone (aqpbf1), contained an insert of 187 ...199910469254
peritrophins of adult dipteran ectoparasites and their evaluation as vaccine antigens.several peritrophins of larvae of lucilia cuprina (sheep blowfly) have demonstrated potential as vaccine antigens, and some have been characterised and cloned. these proteins are tightly associated with the peritrophic matrix, a chitinous tube or sac lining the lumen of the gut of most insects. the peritrophins require strong denaturants for their removal from peritrophic matrix. we now report the preliminary characterisation of peritrophins of the adult stage of l. cuprina and haematobia irrita ...199910579425
estimation of the effects of buffalo fly (haematobia irritans exigua) on the milk production of dairy cattle based on a meta-analysis of literature data.published reports on the effect of buffalo fly haematobia irritans exigua de meijere (diptera: muscidae) and the closely related horn fly (h. irritans) were examined and analysed using non-linear weighted regression techniques in an attempt to establish the relationship between daily production loss (d), average number of parasites (n) and the average damage per parasite per day (d), and to provide estimates of expected losses in milk yield (myd) and live-weight gain (lwg) in dairy cattle. a mit ...199910608225
possible risk factors on queensland dairy farms for acaricide resistance in cattle tick (boophilus microplus).a case control study was carried out within a cross-sectional survey designed to investigate the management by queensland dairy farmers of the cattle tick boophilus microplus. although 199 farmers were surveyed, data on acaricide resistance were only obtained from 66 farms. multiple models were used to predict the probability of acaricide resistance associated with 30 putative risk factors. the region of the state in which the farm was located and the frequency of acaricide application were cons ...200010681025
using genetics to control cattle parasites-the rockhampton experience.ever since their accidental introduction, cattle ticks, gastrointestinal nematodes and buffalo flies have been major parasites of cattle in northern australia. enormous effort and resources have been directed at chemical control of these parasites but the problem persists in undiminished form. the principal control measure remains the use of breeds that have some degree of parasite resistance. no breed is completely resistant and all are at times adversely affected by parasites. complete resista ...200010719118
cloning of an aquaporin-like cdna and in situ hybridization in adults of the mosquito aedes aegypti (diptera: culicidae).a cdna encoding a putative water channel protein, aquaporin, was cloned from a cdna library of aedes aegypti malpighian tubules. the cdna encodes a 26.11 kda protein similar to insect aquaporins from haematobia irritans exigua (diptera) and cicadella viridis (homoptera), and to mammalian aquaporin 4. localization of the messenger rna (mrna) was performed by in situ hybridization of malpighian tubules and analysed by fluorescence and confocal microscopy. the mrna was localized in tracheolar cells ...200010971718
control of the buffalo fly haematobia irritans exigua in india, using the pyrethroids deltamethrin and fenvalerate.the effectiveness of two pyrethroids, fenvalerate and deltamethrin, against the fly haematobia irritans exigua de meijere (diptera: muscidae) on buffalo was considered in a field trial. fenvalerate provided 100% control for 1, 2 and 4 weeks at concentrations of 0.03, 0.04 and 0.05%, respectively. concentrations of 0.01 and 0.02% were less effective. one hundred percent control of this fly was obtained with deltamethrin for 2, 3 and 6 weeks at concentrations of 0.003, 0.004 and 0.005%, respective ...200111583455
molecular cloning and expression of the dihydrofolate reductase (dhfr) gene from adult buffalo fly (haematobia irritans exigua): effects of antifolates.the folate analogues methotrexate, aminopterin and pyrimethamine were toxic when fed in a blood meal to adult buffalo flies (haematobia irritans exigua), but aminopterin caused greater mortality than methotrexate, while trimethoprim was not toxic to adult flies. this is the first recorded instance of mortality in adult insects caused by ingestion of folate analogues. in order to investigate the mechanism of this toxicity, the dihydrofolate reductase (dhfr) gene was cloned from adult buffalo fly ...200312653939
digestion of host immunoglobulin and activity of midgut proteases in the buffalo fly haematobia irritans exigua.the digestion of blood by the buffalo fly (haematobia irritans exigua) was monitored for 6h at 33 degrees c after a single meal. following the meal, the concentration of soluble protein within the midgut increased to a peak at 2 hours then decreased steadily over the next 4h. the magnitude of the increase in soluble protein at 2h indicated a release of protein from another source; most likely from lysed red blood cells. the immunoglobulin (igg) fraction of the blood meal was digested rapidly (50 ...199812770163
cuticular hydrocarbons of buffalo fly, haematobia exigua, and chemotaxonomic differentiation from horn fly, h. irritans.we determined the quantity and chemical composition of cuticular hydrocarbons of different strains, sexes, and ages of buffalo flies, haematobia exigua. the quantity of cuticular hydrocarbons increased from less than 1 microg/fly for newly emerged flies to over 11 microg/fly in 13-d-old flies. the hydrocarbon chain length varied from c(21) to c(29), with unbranched alkanes and monounsaturated alkenes the major components. newly emerged flies contained almost exclusively c(27) hydrocarbons. incre ...200516195854
wolbachia infection in australasian and north american populations of haematobia irritans (diptera: muscidae).buffalo fly (haematobia irritans exigua) is a major pest of beef and dairy cattle in northern australia. global warming is expected to increase the southern range of buffalo fly. chemical control is compromised by resistance and may not be feasible in extensive production systems and there is rapidly growing market preference for beef and dairy products produced in low-chemical systems. wolbachia are vertically transmitted intracellular bacteria that can profoundly influence host reproduction an ...200919345018
recent developments in tick and buffalo-fly control. 194720262398
recent developments in the control of cattle tick and buffalo-fly. 194720262399
the southward spread of the buffalo fly (lyperosia exigua) and control methods adopted in queensland. 194521005470
molecular and functional characterisation of resilin across three insect orders.resilin is an important elastomeric protein of insects, with roles in the storage and release of energy during a variety of different functional categories including flight and jumping. to date, resilin genes and protein function have been characterised only in a small number of flying insects, despite their importance in fleas and other jumping insects. microscopy and immunostaining studies of resilin in flea demonstrate the presence of resilin pads in the pleural arch at the top of the hind le ...201121878390
parasiticide resistance in flies, lice and ticks in new zealand and australia: mechanisms, prevalence and prevention.this review outlines the history of parasiticide resistance in the principal ectoparasites of sheep and cattle in new zealand and australia, blowflies, buffalo fly (haematobia irritans exigua), sheep biting louse (bovicola ovis) and cattle ticks, and discusses recent changes in their response to insecticides and acaricides. mechanisms of resistance and evaluation methods are described, with ways in which insecticide resistance can be avoided or ameliorated also discussed. resistance in sheep blo ...201525185062
use of coi, cytb and nd5 genes for intra- and inter-specific differentiation of haematobia irritans and haematobia exigua.a multilocus sequence analysis using mitochondria-encoded cytochrome c oxidase subunit i (coi), cytochrome b (cytb), nadh dehydrogenase subunit 5 (nd5); nuclear encoded 18s ribosomal rna (18s) and 28s ribosomal rna (28s) genes was performed to determine the levels of genetic variation between the closely related species haematobia irritans linnaeus and haematobia exigua de meijere. among these five genes, nd5 and cytb genes were found to be more variable and informative in resolving the interspe ...201424912955
contrasting evolutionary patterns between two haplogroups of haematobia exigua (diptera: muscidae) from the mainland and islands of southeast asia.uncovering the hidden diversity and evolutionary history of arthropods of medico-veterinary importance could have significant implications for vector-borne disease control and epidemiological intervention. the buffalo fly haematobia exigua is an obligate bloodsucking ectoparasite of livestock. as an initial step towards understanding its population structures and biogeographic patterns, we characterized partial cytochrome c oxidase subunit i (coi) and cytochrome b (cytb) sequences of h. exigua f ...201728724923
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