| cdna cloning of a sorghum pathogenesis-related protein (pr-10) and differential expression of defense-related genes following inoculation with cochliobolus heterostrophus or colletotrichum sublineolum. | a sorghum cdna clone was isolated by differential screening of a cdna library prepared from mesocotyls (cultivar dk18) inoculated with fungal pathogenes. the deduced translation product shows sequence similarity to a family of intracellular pathogenesis-related proteins (pr-10) with a potential ribonuclease function. we studied the accumulation of pr-10 and chalcone synthase (chs) transcripts in mesocotyls following inoculation with cochliobolus heterostrophus or colletotrichum sublineolum. chs ... | 1999 | 10356799 |
| a stilbene synthase gene (sbsts1) is involved in host and nonhost defense responses in sorghum. | a chalcone synthase (chs)-like gene, sbchs8, with high expressed sequence tag abundance in a pathogen-induced cdna library, was identified previously in sorghum (sorghum bicolor). genomic southern analysis revealed that sbchs8 represents a single-copy gene. sbchs8 expression was induced in sorghum mesocotyls following inoculation with cochliobolus heterotrophus and colletotrichum sublineolum, corresponding to nonhost and host defense responses, respectively. however, the induction was delayed by ... | 2005 | 15821144 |
| colletotrichum sublineolum genetic instability assessed by mutants resistant to chlorate. | the fungus colletotrichum sublineolum, causal agent of sorghum anthracnose, presents high variability, genetic instability and host specialization. the aims of the present work were to investigate the mechanisms involved in the genetic instability in this species. mutants resistant to chlorate and unable to use nitrate (nit mutants), were obtained spontaneously, isolated and characterized for complementation pattern, reversion frequency and rapd profile. the results showed that chlorate-resistan ... | 2007 | 17158042 |
| accumulation of trans-piceid in sorghum seedlings infected with colletotrichum sublineolum. | sorghum sbsts1, a pathogen inducible gene, was previously demonstrated to encode an enzyme with stilbene synthase activity. in this study, we attempt to identify the stilbene derivatives that accumulate in infected sorghum seedlings after inoculation with the anthracnose pathogen colletotrichum sublineolum. scanning for precursor ions that produced the common stilbene aglycones as diagnostic ions was performed in a triple quadrupole mass spectrometer. it was found that infected sorghum seedlings ... | 2008 | 17963800 |
| simultaneous temporal progress of sorghum anthracnose and leaf blight in crop mixtures with disparate patterns. | field studies were conducted at alupe in western kenya in 1995 and 1996 to evaluate the efficacy of crop and species mixtures for the management of sorghum anthracnose (caused by colletotrichum sublineolum) and leaf blight (caused by exserohilum turcicum). the progress of these diseases developing simultaneously on a susceptible sorghum cultivar planted in inter- or intra-row mixtures of varying proportions with either maize or resistant sorghum was monitored. the effects of host type and mixtur ... | 2001 | 18944028 |
| ultrastructure of the infection of sorghum bicolor by colletotrichum sublineolum. | abstract ultrastructural studies of the infection of susceptible and resistant cultivars of sorghum bicolor by colletotrichum sublineolum were conducted. initial penetration events were the same on both susceptible and resistant cultivars. germ tubes originating from germinated conidia formed globose, melanized appressoria, that penetrated host epidermal cells directly. appressoria did not produce appressorial cones, but each penetration pore was surrounded by an annular wall thickening. inward ... | 2001 | 18944388 |
| chitinolytic activity of endophytic streptomyces and potential for biocontrol. | biological sources for the control of plant pathogenic fungi remain an important objective for sustainable agricultural practices. actinomycetes are used extensively in the pharmaceutical industry and agriculture owing to their great diversity in enzyme production. in the present study, therefore, we evaluated chitinase production by endophytic actinomycetes and the potential of this for control of phytopathogenic fungi. | 2008 | 19120915 |
| infection biology and defence responses in sorghum against colletotrichum sublineolum. | to investigate the infection biology of colletotrichum sublineolum (isolate cp2126) and defence responses in leaves of resistant (sc146), intermediately resistant (sc326) and susceptible (btx623) sorghum genotypes. | 2009 | 19302494 |
| identification of flavone phytoalexins and a pathogen-inducible flavone synthase ii gene (sbfnsii) in sorghum. | following inoculation with the anthracnose pathogen colletotrichum sublineolum, seedlings of the sorghum resistant cultivar sc748-5 showed more rapid and elevated accumulation of luteolin than the susceptible cultivar btx623. on the other hand, apigenin was the major flavone detected in infected btx623 seedlings. luteolin was demonstrated to show stronger inhibition of spore germination of c. sublineolum than apigenin. because of their pathogen-inducible and antifungal nature, both flavone aglyc ... | 2010 | 20007684 |
| flavonoid phytoalexin-dependent resistance to anthracnose leaf blight requires a functional yellow seed1 in sorghum bicolor. | in sorghum bicolor, a group of phytoalexins are induced at the site of infection by colletotrichum sublineolum, the anthracnose fungus. these compounds, classified as 3-deoxyanthocyanidins, have structural similarities to the precursors of phlobaphenes. sorghum yellow seed1 (y1) encodes a myb transcription factor that regulates phlobaphene biosynthesis. using the candystripe1 transposon mutagenesis system in sorghum, we have isolated functional revertants as well as loss-of-function alleles of y ... | 2010 | 20083611 |
| two loci in sorghum with nb-lrr encoding genes confer resistance to colletotrichum sublineolum. | the aim of this work was to identify plant resistance genes to the sorghum anthracnose fungus colletotrichum sublineolum. cdna-aflp transcript profiling on two contrasting sorghum genotypes inoculated with c. sublineolum generated about 3,000 informative fragments. in a final set of 126 sequenced genes, 15 were identified as biotic stress related. seven of the plant-derived genes were selected for functional analysis using a brome mosaic virus-based virus-induced gene silencing (vigs) system fol ... | 2011 | 22143275 |