neoxaline, a new alkaloid produced by aspergillus japonicus. production, isolation and properties.a new alkaloid named neoxaline has been isolated from culture broth of aspergillus japonicug fg-551 by solvent extraction and silica gel chromatography. the compound does not possess antimicrobial activities, but weakly stimulates the central nervous system. the molecular formula of neoxaline has been determined as c23h25n5o4 on the basis of elemental analysis and its mass spectrometry.1979500498
in vitro and in vivo inhibition of cysteine proteinases by est, a new analog of e-64.e-64 isolated from a culture of aspergillus japonicus is a specific inhibitor of cysteine proteinases. e-64-c, a synthetic analog of e-64, was effective in model animals of muscular dystrophy only when it was given intraperitoneally and by means of osmotic minipump. it showed no effects due to its low absorbability from intestine when it was administered orally. est, the ethyl ester of e-64-c, was expected to be readily absorbed through intestinal membrane, since it is more lipophilic than e-64- ...19863023601
effects of light, temperature and nutrients on the production of conidia and sclerotia by forms of aspergillus japonicus. 19655871071
common environmental allergens causing respiratory allergy in india.respiratory allergy affects all age groups but the children are the worst affected by the respiratory allergy. bioparticles from different biological sources are the main cause of allergy. pollen grains, fungal spores, insect and other materials of biological origin form the most important allergen load in the air. for the efficient diagnosis of the allergy and its effective treatment it is very important to know about the prevalence, seasonal and annual variations of aeroallergens of the area. ...200212003301
functional analysis of the copper-dependent quercetin 2,3-dioxygenase. 2. x-ray absorption studies of native enzyme and anaerobic complexes with the substrates quercetin and myricetin.quercetin 2,3-dioxygenase (2,3qd) is a mononuclear copper-dependent dioxygenase which catalyzes the cleavage of the heterocyclic ring of the flavonol quercetin (5,7,3',4'-tetrahydroxy flavonol) to produce 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. in this study, x-ray absorption spectroscopy has been used to characterize the local structural environment of the cu(2+) center of aspergillus japonicus 2,3qd. analysis of the exafs region of native 2,3qd at functionally rel ...200212069586
epr characterization of the mononuclear cu-containing aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates.quercetin 2,3-dioxygenase (2,3qd) is a copper-containing dioxygenase that catalyses the oxidation of the flavonol quercetin to 2-protocatechuoylphloroglucinol carboxylic acid with concomitant production of carbon monoxide. in contrast to iron dioxygenases, very little is known about copper dioxygenases. we have characterized 2,3qd from the fungus aspergillus japonicus by electron paramagnetic resonance spectroscopy (epr). at ph 6.0, 2,3qd shows a mixture of two epr species. the major form has pa ...200212071961
functional analysis of the copper-dependent quercetin 2,3-dioxygenase. 1. ligand-induced coordination changes probed by x-ray crystallography: inhibition, ordering effect, and mechanistic insights.the crystal structures of the copper-dependent aspergillus japonicus quercetin 2,3-dioxygenase (2,3qd) complexed with the inhibitors diethyldithiocarbamate (ddc) and kojic acid (koj) are reported at 1.70 and 2.15 a resolution, respectively. both inhibitors asymmetrically chelate the metal center and assume a common orientation in the active site cleft. their molecular plane blocks access to the inner portion of the cavity which is lined by the side chains of residues met51, thr53, phe75, phe114, ...200212069585
molecular and phenotypic characterization of aspergillus japonicus and aspergillus aculeatus strains with special regard to their mitochondrial dna polymorphisms.forty aspergillus japonicus and a. aculeatus strains, most of them wild-type isolates, were examined using various molecular and phenotypic techniques. the rdnas proved to be invariable (even strains of the species a. aculeatus exhibited the same restriction profile), while the strains could be classified into seven different mtdna rflp groups. hybridisation data suggest that six of these mtdna types have certain common restriction sites, while mtdna type 7, which was exhibited by some a. aculea ...19979442274
immobilization of beta-fructofuranosidases from aspergillus on methacrylamide-based polymeric beads for production of fructooligosaccharides.beta-fructofuranosidases from aspergillus niger atcc 20611 and aspergillus japonicus tit-kj1 were purified and immobilized covalently onto methacrylamide-based polymeric beads. the porous, oxriane-containing support was reactive and could bind enzymes in a buffered solution at room temperature with a density up to 0.4 mg of protein g-1 of support with 100% immobilized yield. neither the optimum temperature for the highest enzymatic activities nor the batch reaction pattern for fructooligosacchar ...19979336977
recovery and properties of a fructooligosaccharides-producing beta-fructofuranosidase from aspergillus japonicus ccrc 38011.the fungus (aspergillus japonicus ccrc 38011) was found to be able to produce beta-fructofuranosidase with high transfructosylating activity (ut), a key enzyme involved in synthesis of fructooligosaccharides from sucrose. the ut productivities of this microorganism were 191.5 units/ml broth in a 6-l jar-fermentor and 256.1 units/ml broth in a 1000-l pilot-fermentor in a modified medium containing 8% sucrose as a carbon source. most of the ut of this microorganism was found to be bound with mycel ...19937809276
preparation of a new arabinoxylooligosaccharide from wheat bran hemicellulose and its structure.a novel arabinoxylooligosaccharide was prepared from wheat bran and its structure was analyzed. wheat bran hemicellulose was digested with a commercial aspergillus japonicus hemicellulase preparation and an oligosaccharide was purified by carbon column and gel filtration chromatographies to be a single component. the oligosaccharide has d.p. of 6 and contains arabinose and xylose with a molar ratio of 2:1. xylotetraose was formed as an intermediate hydrolyzate on acid hydrolysis of this oligosac ...19947764541
catabolism of benzoate and salicylate by aspergillus japonicus. 19846480052
degradation of myelin proteins by cathepsin b and inhibition by e-64 analogue.purified human brain myelin was isolated, heat-treated to inactivate the endogenous proteolytic activity and incubated with cathepsin b purified from rat liver, at ph 6.0. incubation resulted in a marked reduction of myelin basic protein (bp) and partial breakdown of proteolipid protein or wolfgram protein. degradation of myelin proteins was inhibited by e-64 analogue (e-64-a). e-64 is a specific thiol protease inhibitor isolated from a solid culture of aspergillus japonicus. the present study s ...19846472569
glycerol oxidase, a novel copper hemoprotein from aspergillus japonicus. molecular and catalytic properties of the enzyme and its application to the analysis of serum triglycerides.glycerol oxidase purified from aspergillus japonicus at 008 had mr = 400,000 and contained 1 mol of protoheme ix and 2 g atoms of copper/mol of enzyme protein. the absorption maxima of the oxidized form were found at 557, 530, 420, 280, and 238 nm, and those of the reduced form at 557 and 430 nm. anaerobic addition of glycerol to the enzyme produced both a shift of the soret band from 420 to 410 nm and bleaching of the alpha and beta bands at 557 and 530 nm. the esr spectrum of glycerol oxidase ...19846321480
glycerol oxidase from aspergillus japonicus. 19826292659
beneficial effect of new thiol protease inhibitors, epoxide derivatives, on dystrophic mice.we studied the effect of e-64, a new thiol protease inhibitor derived from aspergillus japonicus tpr-64, and of its synthesized analogue, e-64-d, on dystrophic mice (c57bl/6j-dy). the locomotor activity of normal mice increased markedly, reaching a plateau at 8 weeks of age. in dystrophic mice, it increased until they were 7 weeks old, thereafter, it decreased gradually. this decrease reflected the degradation of the hind legs. serum activities of creatine phosphokinase were significantly greate ...19863940877
different patterns of protein kinase c redistribution mediated by alpha 1-adrenoceptor stimulation and phorbol ester in rat isolated left ventricular papillary muscle.1. in rat left ventricular papillary muscle, phenylephrine, an alpha 1-adrenoceptor agonist, had a staurosporine-sensitive positive inotropic effect and increased the particulate-associated protein kinase c (pkc) activity without significant changes in total pkc activity or in cytosolic ca2+/phospholipid-independent kinase (pki) activity. 2. a pkc stimulant, phorbol 12,13-dibutyrate (pdbu), decreased contractility and slightly increased pkc activity in the particulate fractions, with a marked de ...19921358384
ochratoxigenic species from spanish wine grapes.the ochratoxigenic mycobiota of grapes belonging to representative wine regions located along the mediterranean coast of spain at different developmental stages was identified. during the development of the berries, the occurrence of aspergillus spp. increased while the percentage of berries contaminated by non-ochratoxin a (ota) producing species such as alternaria spp. and cladosporium spp. decreased. penicillium verrucosum, the only confirmed penicillium spp. that is able to produce ota, was ...200515681040
differences in concentrations of allergenic pollens and spores at different heights on an agricultural farm in west bengal, india.the aim of the study was to assess the vertical profile of the major airborne pollen and spore concentration in the lower heights (up to six meters) and to check their allergenic potential causing respiratory allergy in agricultural workers. the study was conducted using rotorod samplers mounted at different heights at weekly intervals for two consecutive years (november 1997-october 1999). the major pollen grains and fungal spores (from mass culture) were collected in bulk and studied by skin-p ...200111748868
intron mobility results in rearrangement in mitochondrial dnas of heterokaryon incompatible aspergillus japonicus strains after protoplast fusion.mitochondrial transmission was carried out under selective conditions between incompatible aspergillus japonicus strains always using an oligomycin-resistant mitochondrial donor and selecting for recipient nuclei and oligomycin-resistant mitochondria. all attempted intraspecific mitochondrial transmissions were successful, but the transmission between closely related a. japonicus and a. aculeatus failed. under selection pressure, resistant progeny harbor the mitochondrial dna (mtdna) of the dono ...200111456461
combined molecular and biochemical approach identifies aspergillus japonicus and aspergillus aculeatus as two species.we examined nine aspergillus japonicus isolates and 10 aspergillus aculeatus isolates by using molecular and biochemical markers, including dna sequences of the its1-5.8s rrna gene-its2 region, restriction fragment length polymorphisms (rflp), and secondary-metabolite profiles. the dna sequence of the internal transcribed spacers (its1 and its2) and the 5.8s rrna gene could not be used to distinguish between a. japonicus and a. aculeatus but did show that these two taxa are more closely related ...200111157212
purification of active cysteine proteases by affinity chromatography with attached e-64 inhibitor.cysteine proteases are implicated in many regulatory and degradative processes in animal and plant cells. many of the proteases are strongly inhibited by an irreversible inhibitor, trans-(epoxysuccinyl)-l-leucylamino-4-guanidinobutane (e-64) from aspergillus japonicus. here we report a method for purification of cysteine proteases by affinity chromatography on e-64. attachment of the inhibitor to thiopropyl sepharose through its epoxy group resulted in the loss of its irreversible activity but d ...199910092483
purification and characterization of beta-fructofuranosidase from aspergillus japonicus tit-kj1.a beta-fructofuranosidase (ec was purified to homogeneity from aspergillus japonicus tit-kj1. the enzyme had an optimum ph for activity of 5.4 and ph stability at 7.0-8.4. the optimum temperature at ph 5.4 was 60 degrees c. the enzyme had a molecular weight of 236,000 with two subunits and an isoelectric point of ph 4.0. the enzyme was inactivated by 5 mm hg2+ and ag+. the enzyme had a high transfructosylating activity. treatment of 50% (w/v) sucrose with the enzyme under optimum condi ...19937764333
metabolism of n-alkanes by aspergillus japonicus.cells of aspergillus japonicus could degrade n-alkanes as a sole source of carbon. one of the pathways operative during the degradative process was the terminal pathway. electron micrographs showed that the hydrocarbons were present in the cells as microdroplets.19873817657
continuous production of high-content fructooligosaccharides by a complex cell system.a complex biocatalyst system with a bioreactor equipped with a microfiltration (mf) module was employed to produce high-content fructooligosaccharides (fos) in a continuous process initiated by a batch process. the system used mycelia of aspergillus japonicus ccrc 93007 or aureobasidium pullulans atcc 9348 with beta-fructofuranosidase activity and gluconobacter oxydans atcc 23771 with glucose dehydrogenase activity. calcium carbonate slurry was used to control ph to 5.5, and gluconic acid in the ...200212467463
crystal structure of the copper-containing quercetin 2,3-dioxygenase from aspergillus japonicus.quercetin 2,3-dioxygenase is a copper-containing enzyme that catalyzes the insertion of molecular oxygen into polyphenolic flavonols. dioxygenation catalyzed by iron-containing enzymes has been studied extensively, but dioxygenases employing other metal cofactors are poorly understood. we determined the crystal structure of quercetin 2,3-dioxygenase at 1.6 a resolution. the enzyme forms homodimers, which are stabilized by an n-linked heptasaccharide at the dimer interface. the mononuclear type 2 ...200211839311
oxidation of ethylene glycol and glycolic acid by glycerol oxidase.a glycerol oxidase from aspergillus japonicus oxidized ethylene glycol to glyoxal by the same reaction pathway as alcohol oxidases from methanol yeast. the optimum ph and temperature for the oxidation of ethylene glycol were around 7.0 and 40 degrees c, respectively. those of glycolaldehyde were similar to those of ethylene glycol. the apparent kms for ethylene glycol and glycolaldehyde were 195 and 48.8 mm, respectively. the maximum velocities for ethylene glycol and glycolaldehyde were 89.1 an ...19957772820
production of beta-fructofuranosidase with transfructosylating activity for fructooligosaccharides synthesis by aspergillus japonicus ntu-1249.microbial beta-fructofuranosidases with transfructosylating activity can catalyze the transfructosylation of sucrose and synthesize fructooligosaccharides. aspergillus japonicus ntu-1249 isolated from natural habitat was found to produce a significant amount of beta-fructofuranosidase with high transfructosylating activity and to have the potential for industrial production of fructooligosaccharides. in order to improve it's enzyme productivity, the medium composition and the cultivation conditi ...19911819045
mycotoxin production on rice, pulses and oilseeds.mycotoxin-producing fungi were isolated from contaminated grains of rice, pulses and oilseeds sold in the local markets of calcutta for human consumption. it was found that aflatoxin b1 was produced by aspergillus flavus and aspergillus parasiticus, aflatoxin g1 by a. flavus, ochratoxin by aspergillus ochraceous, sterigmatocystin by aspergillus japonicus and citrinin by penicillium citrinum. aflatoxin b1 (333-10,416 micrograms/kg) was produced by aspergillus spp. in rice, pulses and oilseeds.200010929292
[allelopathic effects of secalonic acid f produced by aspergillus japonicus on zea mays].aspergillus japonicus saito is a common soil-borne fungus, and exists on the surface of some cereal crop seeds. secalonic acid f (saf) is the main allelochemical from the fungus. bioassays showed that saf significantly stimulated the seedling growth of corn (zea mays l.) at a concentration of 0.0375 mmol.l-1. the root length, root numbers and root oxidation activities increased by 31.7%, 13.2%, and 373%, respectively. secalonic acid f also increased the nutrient absorption of p, k, ca, mg, and s ...200415139208
airborne viable, non-viable, and allergenic fungi in a rural agricultural area of india: a 2-year study at five outdoor sampling stations.the information on airborne allergenic fungal flora in rural agricultural areas is largely lacking. adequate information is not available to the bioaerosol researchers regarding the choice of single versus multiple sampling stations for the monitoring of both viable and non-viable airborne fungi. there is no long-term study estimating the ratios of viable and non-viable fungi in the air and earlier studies did not focus on the fractions of airborne allergenic fungi with respect to the total airb ...200415142771
dna-based characterization of ochratoxin-a-producing and non-producing aspergillus carbonarius strains from grapes.using molecular methods, a total of 189 strains of black aspergilli, including aspergillus carbonarius and uniseriate species (aspergillus aculeatus, aspergillus japonicus), were studied in order to characterize species responsible for ochratoxin a (ota) contamination of grapes from europe and israel. sixty-six strains were morphologically identified as belonging to the uniseriate species and 123 as a. carbonarius. none of the uniseriate species were able to produce ota. from the a. carbonarius ...200515808942
a new monocupin quercetinase of streptomyces sp. fla: identification and heterologous expression of the qued gene and activity of the recombinant enzyme towards different flavonols.the gene qued encoding quercetinase of streptomyces sp. fla, a soil isolate related to s. eurythermus (t), was identified. quercetinases catalyze the 2,4-dioxygenolytic cleavage of 3,5,7,3',4'-pentahydroxyflavone to 2-protocatechuoylphloroglucinol carboxylic acid and carbon monoxide. the qued gene was expressed in s. lividans and e. coli, and the recombinant hexahistidine-tagged protein (quedhis(6)) was purified. several flavonols were converted by quedhis(6), whereas co formation from the 2,3-d ...200717516049
anaerobic enzyme.substrate structures provide insight into the reaction mechanism of the copper-dependent quercetin 2,3-dioxygenase.quercetin 2,3-dioxygenase (2,3qd) is the only firmly established copper dioxygenase known so far. depending solely on a mononuclear cu center, it catalyzes the breakage of the o-heterocycle of flavonols, producing more easily degradable phenolic carboxylic acid ester derivatives. in the enzymatic process, two cc bonds are broken and concomitantly carbon monoxide is released. the x-ray structures of aspergillus japonicus 2,3qd anaerobically complexed with the substrate kaempferol and the natural ...200212486225
characterization of cold- and high-pressure-active polygalacturonases from a deep-sea yeast, cryptococcus liquefaciens strain n6.a deep-sea yeast, cryptococcus liquefaciens strain n6, produces two polygalacturonases, p36 and p40 (n6-pgases). these n6-pgases were highly active at 0-10 degrees c in comparison to a pgase from aspergillus japonicus. the hydrolytic activity of these n6-pgases remained almost unchanged up to a hydrostatic pressure of 100 mpa at 24 degrees c with a very small activation volume of -1.1 ml/mol. at 10 degrees c, however, the activation volume increased to 3.3 or 5.4 ml/mol (p36 and p40, respectivel ...200616428855
analysis of the components released from potato tuber tissues during maceration by pectolytic enzymes.endo-pectin lyase and endo-polygalacturonase of aspergillus japonicus attack the middle lamella of plant tissue and cause tissue maceration. galacturonides, neutral sugars, and proteins were released from potato tuber tissues during maceration by both purified enzymes. these three components accounted for 92% of the soluble products. the neutral sugars released were rhamnose, arabinose, and galactose with a molar ratio of 1:3:15. they were covalently linked to galacturonides. over 85% of the gal ...197816660564
cell wall cementing materials of grass leaves.treatment of grass leaves with either a purified pectin lyase of aspergillus japonicus or a purified xylanase of trichoderma viride could lead to the isolation of some single leaf cells. however, a mixture of pectin lyase and xylanase brought about more rapid isolation of single cells than did either of the two enzymes alone, indicating a synergistic effect. analysis of the components released from oat cell walls by the enzymes indicated that both homogalacturonans with a high degree of esterifi ...198416663980
cellulase complex of the fungus chrysosporium lucknowense: isolation and characterization of endoglucanases and cellobiohydrolases.using different chromatographic techniques, eight cellulolytic enzymes were isolated from the culture broth of a mutant strain of chrysosporium lucknowense: six endoglucanases (eg: 25 kd, pi 4.0; 28 kd, pi 5.7; 44 kd, pi 6.0; 47 kd, pi 5.7; 51 kd, pi 4.8; 60 kd, pi 3.7) and two cellobiohydrolases (cbh i, 65 kd, pi 4.5; cbh ii, 42 kd, pi 4.2). some of the isolated cellulases were classified into known families of glycoside hydrolases: cel6a (cbh ii), cel7a (cbh i), cel12a (eg28), cel45a (eg25). i ...200415193129
expression and characterization of aspergillus thermostable phytases in pichia pastoris.two thermostable phytases were identified from thai isolates of aspergillus japonicus bcc18313 (tr86) and aspergillus niger bcc18081 (tr170). both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into pichia pastoris. the recombinant phytases, r-phya86 and r-phya170, were expressed as active extracellular, glycosylated proteins with activities of 140 and 100 u ml(-1), respectively. both recombinant phytases exhibited high affinity for ...200919025560
specific xyloglucanases as a new class of polysaccharide-degrading enzymes.three specific xyloglucanases (xgs) were isolated from aspergillus japonicus (32 kda, pi 2.8), chrysosporium lucknowense (78 kda, pi 3.8) and trichoderma reesei (75-105 kda, pi 4.1-4.3). the characteristic feature of these enzymes was their high specific activity toward tamarind xyloglucan, whereas the activity against carboxymethylcellulose (cmc) and barley beta-glucan was absent or very low. peptide mass fingerprinting using maldi-tof mass spectrometry showed that the t. reesei xg represents c ...200415541296
new paralytic alkaloids, asperparalines a, b and c, from aspergillus japonicus paralytic alkaloids, asperparalines a (1), b (2) and c (3), were isolated from okara (the insoluble residue of whole soybean) that had been fermented with aspergillus japonicus jv-23. their structures were elucidated by spectroscopic methods and x-ray crystallography. these asperparalines showed paralytic activity against silk worms.200010705455
immobilization of beta-fructofuranosidase from aspergillus japonicus on chitosan using tris(hydroxymethyl)phosphine or glutaraldehyde as a coupling agent.a partially purified beta-fructofuranosidase from aspergillus japonicus was covalently immobilized on to chitosan beads using either glutaraldehyde or tris(hydroxymethyl)phosphine (thp) as a coupling agent. compared with the glutaraldehyde-immobilized and the free enzyme, the thp-immobilized enzyme had the highest thermal stability with 78% activity retained after 12 days at 37 degrees c. the thp-immobilized enzyme also had higher reusability than that immobilized by glutaraldehyde, 75% activity ...200515834795
conidia of black aspergilli as new biological adsorbents for ochratoxin a in grape juices and musts.biological removal of ochratoxin a (ota) by living and heat-treated dead conidia of black aspergillus isolates representing the species aspergillus niger, aspergillus carbonarius, and aspergillus japonicus in synthetic and natural grape juices was found to be a two-stage phenomenon. several lines of evidence suggest that the first observed stage was passive, metabolism was not required, and ota adsorption on conidia of black aspergilli could be involved. this removal was fast, without delay just ...200516218668
fungal biodegradation and biotransformation of soluble lignocarbohydrate complexes from straw.aspergillus japonicus is an efficient degrader of phenolics and carbohydrates present in a mixture of soluble lignocarbohydrate complexes extracted from wheat straw. trichoderma sp. attacked part of the carbohydrate but hardly affected the aromatic portion of this solution. polyporus versicolor had a complex effect; polymerization of low-molecular-size phenolics accompanied the degradation of aromatic and carbohydrate polymers. the addition of xylose to the medium facilitated depolymerization of ...198316346352
efficient total synthesis of novel bioactive microbial metabolites.bioactive natural products produced by microbes have almost limitless potential in pharmaceutical applications, and the organic synthesis of such products as lead compounds will result in the creation of new and widely useful pharmaceutical products. a program of discovery of naturally occurring bioactive microbial metabolites has been ongoing at the kitasato institute. we have also developed efficient, rational, and highly flexible production methods for generation of target compounds, synthesi ...200818217720
restoration of mature etiolated cucumber hypocotyl cell wall susceptibility to expansin by pretreatment with fungal pectinases and egta in vitro.mature plant cell walls lose their ability to expand and become unresponsive to expansin. this phenomenon is believed to be due to cross-linking of hemicellulose, pectin, or phenolic groups in the wall. by screening various hydrolytic enzymes, we found that pretreatment of nongrowing, heat-inactivated, basal cucumber (cucumis sativus) hypocotyls with pectin lyase (pel1) from aspergillus japonicus could restore reconstituted exogenous expansin-induced extension in mature cell walls in vitro. reco ...200818562768
new monolithic enzymatic micro-reactor for the fast production and purification of production and purification of alpha-(1,4)-oligogalacturonides was investigated using a new enzymatic reactor composed of a monolithic matrix. pectin lyase from aspergillus japonicus (sigma) was immobilized on cim-disk epoxy monolith. studies were performed on free pectin lyase and immobilized pectin lyase to compare the optimum temperature, optimum ph, and thermal stability. it was determined that optimum temperature for free pectin lyase and immobilized pectin lyase on monolithic support ...200817870676
elicitor-inducible 3-hydroxy-3-methylglutaryl coenzyme a reductase activity is required for sesquiterpene accumulation in tobacco cell suspension cultures.addition of cell wall fragments from phytophthora species or cellulase from trichoderma viride, but not pectolyase from aspergillus japonicus, to tobacco (nicotiana tabacum) cell suspension cultures induced the accumulation of the extracellular sesquiterpenoid capsidiol. pulse-labeling experiments with [(14)c]acetate and [(3)h]mevalonate suggested that enzymatic steps preceding mevalonate were limiting capsidiol biosynthesis in the pectolyase-treated cell cultures. treatment of the cell cultures ...199116668454
preparation of high-purity fructo-oligosaccharides by aspergillus japonicus beta-fructofuranosidase and successive cultivation with yeast.the short-chain foss with high purity were prepared using a two-step strategy: aspergillus japonicus extracellular beta-fructofuranosidase-catalyzed synthesis of foss followed by cultivation with pichia pastoris (p. pastoris). the higher foss content was obtained after 8 h under the catalysis of beta-fructofuranosidase at ph 5.5 and 55 degrees c. successive p. pastoris cultivation exhausts almost all monosugars in 12 h at 30 degrees c, which increases the purity of foss, and also recovers beta-f ...200818333616
[transformation system of aspergillus japonicus mediated by agrobacterium tumefaciens].in to order to select aspergillus japonicus mutant strains that express high-yield of glycerol oxidase, we constructed a mutant library of aspergillus japonicus that mediated by agrobacterium tumefaciens.201121465797
a fungal metabolite asperparaline a strongly and selectively blocks insect nicotinic acetylcholine receptors: the first report on the mode of action.asperparalines produced by aspergillus japonicus jv-23 induce paralysis in silkworm (bombyx mori) larvae, but the target underlying insect toxicity remains unknown. in the present study, we have investigated the actions of asperparaline a on ligand-gated ion channels expressed in cultured larval brain neurons of the silkworm using patch-clamp electrophysiology. bath-application of asperparaline a (10 µm) had no effect on the membrane current, but when delivered for 1 min prior to co-application ...201121483774
biosolubilization of rock phosphate by three stress-tolerant fungal strains.three stress-tolerant phosphate-solubilizing fungal strains identified as aspergillus niger, aspergillus japonicus, and penicillium simplicissimum were isolated from wheat rhizospheric soil. the strains demonstrated different capabilities of phosphate solubilization in national botanical research institute's phosphate medium containing rock phosphate (rp) as sole phosphorus (p) source, and the solubilization of rp by p. simplicissimum was the most effective among these strains, followed by a. ni ...201121625871
biochemical and molecular characterization of a quercetinase from penicillium olsonii.quercetinase (quercetin 2,3-dioxygenase, ec is produced by various filamentous fungi when grown on rutin as the sole carbon and energy source. from a rutin based liquid culture of penicillium olsonii, we purified a quercetinase with a specific activity of 175u mg(-1). the enzyme is a monomeric glycoprotein of approximately 55 kda, containing 0.9+/-0.1 copper atoms per protein. its substrate specificity is restricted to the flavonol family of flavonoids. it is completely inhibited by ...200818206655
aflp characterization of southern europe population of aspergillus section nigri from grapes.members of aspergillus belonging to section nigri are distributed worldwide and are mainly responsible for the ochratoxin a accumulation in grapes and wine, particularly in southern europe. limited information is available on the species composition and genetic variability of black aspergilli strains occurring on grapes. we analyzed 283 representative strains from the main wine producing european countries collected in 2001-2002 (italy, france, spain, portugal, greece and israel) using amplified ...200616682091
single-crystal epr study at 95 ghz of the type 2 copper site of the inhibitor-bound quercetin 2, electron-spin-echo-detected, electron-paramagnetic-resonance study has been performed on the type 2 copper site of quercetin 2,3-dioxygenase from aspergillus japonicus. in the protein, copper is coordinated by three histidine nitrogens and two sulfurs from the inhibitor diethyldithiocarbamate. a single crystal of the protein was studied at 95 ghz and the complete g-tensor determined. the electron-paramagnetic-resonance data are compatible with two orientations of the principal g-axes in the c ...200314645093
rock phosphate solubilization under alkaline conditions by aspergillus japonicus and a. foetidus.aspergillus japonicus and a. foetidus were found to solubilize five types of indian rock phosphates at ph 8 and 9. solubilization was higher in the presence of pyrite than in controls lacking either pyrite or fungal inoculum. both the aspergilli were found to be good pyrite solubilizers and could grow over a wide ph range. solubilization of rock phosphates was the result of organic acid release and pyrite oxidation.19948181779
fungal contamination and aflatoxin b1 and ochratoxin a in lebanese wine-grapes and musts.five hundred and ten strains of filamentous fungi were isolated from lebanese grapes during 2005 at veraison and harvesting periods. four hundred eighty-seven isolates belonged to the aspergillus spp. (95.5%) and 23 belonged to the penicillium spp. (4.5%). black aspergilli constituted 56.9% (52.2% aspergillus niger aggregates, 2.9% aspergillus japonicus and 1.8% aspergillus carbonarius) while the isolation rate of aspergillus flavus the none habitual member of grape mycobiota was 43.1% of the to ...200818430504
description of a cellulose-binding domain and a linker sequence from aspergillus fungi.a family i cellulose-binding domain (cbd) and a serine- and threonine-rich linker peptide were cloned from the fungi aspergillus japonicus and aspergillus aculeatus. a glutathione s-transferase (gst) fusion protein comprising gst and a peptide linker with the cbd fused to its c-terminus, was expressed in escherichia coli. the renatured gst-cbd recovered from inclusion bodies had a molecular mass of 36.5 kda which agrees with the 29 kda of the gst plus the calculated 7.5 kda of the linker with th ...200211956750
survey of philippine coffee beans for the presence of ochratoxigenic 2012 to 2014, philippine green coffee beans from coffea arabica in benguet and ifugao; coffea canephora var. robusta in abra, cavite, and ifugao; and coffea liberica and coffea excelsea from cavite were collected and assessed for the distribution of fungi with the potential to produce ochratoxin a (ota). the presence of fungal species was evaluated both before and after surface sterilization. there were remarkable ecological and varietal differences in the population of ota-producing species ...201626814977
diversity of black aspergilli isolated from raisins in argentina: polyphasic approach to species identification and development of scar markers for aspergillus ibericus.aspergillus section nigri is a heterogeneous fungal group including some ochratoxin a producer species that usually contaminate raisins. the section contains the series carbonaria which includes the toxigenic species aspergillus carbonarius and nontoxigenic aspergillus ibericus that are phenotypically undistinguishable. the aim of this study was to examine the diversity of black aspergilli isolated from raisins and to develop a specific genetic marker to distinguish a. ibericus from a. carbonari ...201526114593
biosorption of metal ions from aqueous solution and electroplating industry wastewater by aspergillus japonicus: phytotoxicity studies. 200616977523
kinetic and spectroscopic studies on the quercetin 2,3-dioxygenase from bacillus subtilis.quercetin 2,3-dioxygenase from bacillus subtilis (qued) converts the flavonol quercetin and molecular oxygen to 2-protocatechuoylphloroglucinolcarboxylic acid and carbon monoxide. qued, the only known quercetin 2,3-dioxygenase from a prokaryotic organism, has been described as an fe2+-dependent bicupin dioxygenase. metal-substituted queds were generated by expressing the enzyme in escherichia coli grown on minimal media in the presence of a number of divalent metals. the addition of mn2+, co2+, ...200616411777
heat and microbial treatments for nutritional upgrading of wheat straw.the ligninolytic activities of four cellulolytic organisms were compared using straw. only aspergillus japonicus and polyporous versicolor appreciably degraded lignin with a. japonicus yielding the most protein. in solid culture, most protein was produced by p. versicolor, closely followed by a. japonicus. pretreatment of the straw by hot water facilitated biodegradation and protein production. the nutritional value of the residual straw was also increased by some fungal cultures. the greatest a ...198618555339
two novel species of aspergillus section nigri from thai coffee beans.two novel species of aspergillus section nigri from thai coffee beans are described as aspergillus aculeatinus sp. nov. and aspergillus sclerotiicarbonarius sp. nov. their taxonomic status was determined using a polyphasic taxonomic approach with phenotypic (morphology and extrolite profiles) and molecular (beta-tubulin, internal transcribed spacer and calmodulin gene sequences) characteristics. a. aculeatinus sp. nov. is a uniseriate species with a similar morphology to aspergillus aculeatus an ...200818599725
aspergillus saccharolyticus sp. nov., a new black aspergillus species isolated in denmark.a novel species, aspergillus saccharolyticus sp. nov., is described within aspergillus section nigri species. this species was isolated in denmark from treated hardwood. its taxonomic status was determined using a polyphasic taxonomic approach with phenotypic (morphology and extrolite profiles) and molecular (beta-tubulin, internal transcribed spacer and calmodulin gene sequences, and universally-primed pcr fingerprinting) characteristics. these features clearly distinguished this species from o ...201121335500
aspergillus uvarum sp. nov., an uniseriate black aspergillus species isolated from grapes in europe.a novel species, aspergillus uvarum sp. nov., is described within aspergillus section nigri. this species can be distinguished from other black aspergilli based on internal transcribed spacers (its), beta-tubulin and calmodulin gene sequences, by aflp analysis and by extrolite profiles. aspergillus uvarum sp. nov. isolates produced secalonic acid, common to other aspergillus japonicus-related taxa, and geodin, erdin and dihydrogeodin, which are not produced by any other black aspergilli. none of ...200818398215
fermentation of rice hull by aspergillus japonicus under ultrasonic pretreatment.the application of ultrasound for treating rice hull used as the fermentation substrate for xylooligosaccharides production was investigated. aspergillus japonicus cy6-1 was selected to produce cellulolytic and xylanolytic enzymes from untreated rice hull (rh) and rice hull treated with ultrasound (usrh-m). the hemicellulose yield was increased to 1.4-fold with ultrasound, and treatment time was greatly shortened from 24h to 1.5h at 80°c and 300w/28khz. the morphology of rh from various pretreat ...201122177967
a lichenase-like family 12 endo-(1-->4)-beta-glucanase from aspergillus japonicus: study of the substrate specificity and mode of action on beta-glucans in comparison with other glycoside hydrolases.using anion-exchange chromatography on source 15q followed by hydrophobic interaction chromatography on source 15 isopropyl, a lichenase-like endo-(1-->4)-beta-glucanase (bg, 28kda, pi4.1) was isolated from a culture filtrate of aspergillus japonicus. the enzyme was highly active against barley beta-glucan and lichenan (263 and 267 u/mg protein) and had much lower activity toward carboxymethylcellulose (3.9 u/mg). the mode of action of the bg on barley beta-glucan and lichenan was studied in com ...200616343463
collagenase produced from aspergillus sp. (ucp 1276) using chicken feather industrial extracellular collagenolytic serine protease was purified from aspergillus sp., isolated from the caatinga biome in northeast brazil by a two-step chromatographic procedure, using an anion-exchanger and gel filtration. the enzyme was produced by submerged fermentation of feather residue as a substrate. the purified collagenase showed a 2.09-fold increase in specific activity and 22.85% yield. the enzyme was a monomeric protein with a molecular mass of 28.7 kda, estimated by an sds-page and ak ...201727808430
detection of vm55599 and preparaherquamide from aspergillus japonicus and penicillium fellutanum: biosynthetic implications.the secondary metabolites vm55599 (4) and preparaherquamide (5) have been identified by lc-ms(n) analysis as natural metabolites in cultures of penicillium fellutanum, whereas preparaherquamide has been identified only in cultures of aspergillus japonicus. in accord with a previous proposal, the identification of both metabolites, which have a diastereomeric relationship, provides indirect support for a unified biosynthetic scheme.200818754595
purification and properties of an extracellular beta-xylosidase from aspergillus japonicus and sequence analysis of the encoding extracellular protein exhibiting beta-xylosidase activity was purified from the culture filtrate of a filamentous fungus, aspergillus japonicus strain mu-2, grown on oat spelt xylan. the purified enzyme was a monomeric glycoprotein with an apparent m(r) of 113.2 kda as estimated by sds-page. beta-xylosidase activity was optimal at ph 4.0 and 70 degrees c. the enzyme also showed beta-glucosidase and alpha-l-arabinofuranosidase activities. the genomic dna and cdna encoding this protein were clo ...200819000618
beta-fructofuranosidase production by repeated batch fermentation with immobilized aspergillus japonicus.the fungus aspergillus japonicus atcc 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of beta-fructofuranosidases (ffase). the assays were performed during eight consecutive cycles that were completed in a total period of 216 h. after each 24-h cycle of fermentation (except for the first cycle, which lasted 48 h), the fermented broth was replaced by fresh medium, and the ffase activity was determined in the replaced medium. ...200919396483
production of fibrolytic enzymes by aspergillus japonicus c03 using agro-industrial residues with potential application as additives in animal feed.solid-state fermentation obtained from different and low-cost carbon sources was evaluated to endocellulases and endoxylanases production by aspergillus japonicus c03. regarding the enzymatic production the highest levels were observed at 30 °c, using soy bran added to crushed corncob or wheat bran added to sugarcane bagasse, humidified with salt solutions, and incubated for 3 days (xylanase) or 6 days (cellulase) with 70% relative humidity. peptone improved the xylanase and cellulase activities ...201021046416
isolation and properties of pectinases from the fungus aspergillus japonicus.using anion-exchange chromatography on different carriers and phenyl-sepharose hydrophobic chromatography, five pectolytic enzymes were isolated from the culture liquid of a mutant strain of aspergillus japonicus: two endo-polygalacturonases (i and ii, 38 and 65 kd, pi 5.6 and 3.3), pectin lyase (50 kd, pi 3.8), and two pectinesterases (i and ii) with similar molecular weights (46 and 47 kd) and the same pi (3.8). the pectinesterases apparently represent two isoforms of the same enzyme. all puri ...200312882638
isolation and properties of fungal beta-glucosidases.using chromatography on different matrixes, three beta-glucosidases (120, 116, and 70 kda) were isolated from enzymatic complexes of the mycelial fungi aspergillus japonicus, penicillium verruculosum, and trichoderma reesei, respectively. the enzymes were identified by maldi-tof mass-spectrometry. substrate specificity, kinetic parameters for hydrolysis of specific substrates, ability to catalyze the transglucosidation reaction, dependence of the enzymatic activity on ph and temperature, stabili ...200919538132
hydrolysis of rosmarinic acid from rosemary extract with esterases and lactobacillus johnsonii in vitro and in a gastrointestinal model.rosmarinic acid (ra) was identified as one of the main components of rosemary extracts and has been ascribed to a number of health benefits. several studies suggested that after ingestion, ra is metabolized by gut microflora into caffeic acid and derivatives. however, only limited information on the microorganisms and enzymes involved in this biotransformation is available. in this study, we investigated the hydrolysis of ra from rosemary extract with enzymes and a probiotic bacterium lactobacil ...200919658402
Quality Attributes of Cupuaçu Juice in Response to Treatment with Crude Enzyme Extract Produced by Aspergillus japonicus 586.Cupuaçu (Theobroma grandiflorum Schum) is an Amazonian Basin native fruit whose fruit pulp is consumed as a juice which presents high density, viscosity, and turbidity. Pectic enzymes, usually yielded by microorganisms, are used to reduce the juice viscosity and turbidity. The present study aims to evaluate the use of pectic enzymes when processing cupuaçu juice. The cupuaçu juice was obtained by using Aspergillus japonicus 586 crude enzyme extract and incubation at 50°C with agitation (140?rpm) ...201122114735
an extracellular endo-1,4-beta-xylanase from aspergillus japonicus: purification, properties, and characterization of the encoding extracellular endo-1,4-beta-xylanase with specific activity of 566 u/mg was purified from the culture filtrate of a filamentous fungus, aspergillus japonicus strain mu-2, grown on oat spelt xylan. the purified enzyme showed a single band on sds-page with an apparent m(r) of 25.1 kda. xylanase activity was optimal at ph 5.0 and 60 degrees c. the xylanase gene (xyna) encoded a 42 residue prepropeptide and a 191 residue mature protein. the xyna protein showed the highest sequence identity of 69% ...201020159568
isolation and properties of xyloglucanases of penicillium sp.using chromatographic technique, xyloglucanase (xg) a (25 kda, pi 3.5, 12th glycosyl hydrolase family) was isolated from the enzyme complex secreted by the mycelial fungus penicillium canescens, and xyloglucanases xg 25 (25 kda, pi 4.1, 12th glycosyl hydrolase family) and xg 70 (70 kda, pi 3.5, 74th glycosyl hydrolase family) were isolated from the enzyme complex of penicillium verruculosum. properties of the isolated enzymes (substrate specificity, optimal ranges of ph and temperature for enzym ...201020331423
crystal structures of aspergillus japonicus fructosyltransferase complex with donor/acceptor substrates reveal complete subsites in the active site for catalysis.fructosyltransferases catalyze the transfer of a fructose unit from one sucrose/fructan to another and are engaged in the production of fructooligosaccharide/fructan. the enzymes belong to the glycoside hydrolase family 32 (gh32) with a retaining catalytic mechanism. here we describe the crystal structures of recombinant fructosyltransferase (ajft) from aspergillus japonicus cb05 and its mutant d191a complexes with various donor/acceptor substrates, including sucrose, 1-kestose, nystose, and raf ...201020466731
highly efficient synthesis of fructooligosaccharides by extracellular fructooligosaccharide-producing enzymes and immobilized cells of aspergillus aculeatus m105 and purification and biochemical characterization of a fructosyltransferase from the this work, aspergillus aculeatus m105 was obtained to produce high extracellular fructooligosaccharide-producing enzyme activity. the maximum yields of fructooligosaccharides produced by its extracellular enzymes and immobilized cells were 67.54 and 65.47% (w/w), respectively. a fructosyltransferase (ftase), aaft32a, was purified from m105. the optimal ph and temperature of aaft32a were ph 5.0-6.0 and 65 °c, respectively. the km, vmax, and kcat values for the transfructosylating activity of a ...201627492129
effect of cd⁺² on phosphate solubilizing abilities and hydrogen peroxide production of soil-borne micromycetes isolated from phragmites australis-rhizosphere.the aims of this work were to evaluate the phosphate-solubilization and hydrogen peroxide (h2o2) production by the soil-borne micromycetes, aspergillus japonicus, penicillium italicum and penicillium dipodomyicola, isolated from phragmites australis rhizosphere and to study the effect of several concentrations of cadmium (cd(2+)) on both variables. our results showed that p. italicum achieved a higher p-solubilization and h2o2 production than a. japonicus and p. dipodomyicola, as only p. italicu ...201626646403
isolation and properties of extracellular beta-xylosidases from fungi aspergillus japonicus and trichoderma reesei.homogeneous beta-xylosidases with molecular mass values 120 and 80 kda (as shown by sds-page), belonging to the third family of glycosyl hydrolases, were isolated by anion-exchange, hydrophobic, and gel-penetrating chromatography from enzyme preparations based on the fungi aspergillus japonicus and trichoderma reesei, respectively. the enzymes exhibit maximal activity in acidic media (ph 3.5-4.0), and temperature activity optimum was 70 degrees c for the beta-xylosidase of a. japonicus and 60 de ...200919916911
lipolytic potential of aspergillus japonicus lab01: production, partial purification, and characterisation of an extracellular lipase.lipolytic potential of aspergillus japonicus lab01 was investigated by describing the catalytic properties and stability of a secreted extracellular lipase. enzyme production was considered high under room temperature after 4 days using sunflower oil and a combination of casein with sodium nitrate. lipase was partially purified by 3.9-fold, resulting in a 44.2% yield using ammonium sulphate precipitation (60%) quantified with superose 12 hr gel filtration chromatography. the activity of the enzy ...201425530954
optimization of fibrolytic enzyme production by aspergillus japonicus c03 with potential application in ruminant feed and their effects on tropical forages hydrolysis.fibrolytic enzyme production by aspergillus japonicus c03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. a 2(3) full factorial composite and response surface methodology were used to design the experiments and analysis of results. tropical forages were hydrolyzed by a. japonicus c03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. optimal production to xylanase was obtained with soybean bran added ...201121647681
sugarcane molasses and yeast powder used in the fructooligosaccharides production by aspergillus japonicus-fcl 119t and aspergillus niger atcc 20611.different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of aspergillus japonicus-fcl 119t and aspergillus niger atcc 20611. moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as alternative nutrients for both strains' cultivation. these media were formulated for analysis of cellular growth, beta-fructosyltransferase and fructooligosaccharides (fos) production. transfructosylati ...200616835781
colonization of aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides.the ability of aspergillus japonicus atcc 20236 to colonize different synthetic materials (polyurethane foam, stainless steel sponge, vegetal fiber, pumice stones, zeolites, and foam glass) and to produce fructooligosaccharides (fos) from sucrose (165 g/l) is described. cells were immobilized in situ by absorption, through direct contact with the carrier particles at the beginning of fermentation. vegetal fiber was the best immobilization carrier as a. japonicus grew well on it (1.25 g/g carrier ...200919251252
effect of water activity, temperature, and mixed fungal spore interactions on ochratoxin a production by aspergillus carbonarius.the purpose of this work was to investigate the potential of two nontoxigenic aspergillus section nigri species (aspergillus tubingensis and aspergillus japonicus) to influence the in vitro ochratoxin a (ota) production of three toxigenic aspergillus carbonarius isolates (ac-28, ac-29, and ac-33) from greek vineyards of different geographical areas. ota accumulation was evaluated by inoculation of 0:100, 25:75, 50:50, 75:25, and 100:0 ratios of mixed spore suspensions on a synthetic grape juice ...201525710154
effects of aromatic compounds on hemicellulose-degrading enzymes in aspergillus japonicus.aspergillus japonicus was grown in the presence of various aromatic compounds at 0.1 and 1 mg/ml, and extracellular xylanase and arabanase activities were measured. some of the aromatic compounds tested, especially at the higher concentration, suppressed the appearance of hemicellulase activities, expressed as xylanase or arabanase. vanillin at 1 mg/ml in the presence of either xylan or araban completely suppressed growth, and guaiacol and p-coumaric acid markedly inhibited the growth of a. japo ...198518553789
extracellular secretion of phytase from transgenic wheat roots allows utilization of phytate for enhanced phosphorus uptake.a significant portion of organic phosphorus comprises of phytates which are not available to wheat for uptake. hence for enabling wheat to utilize organic phosphorus in form of phytate, transgenic wheat expressing phytase from aspergillus japonicus under barley root-specific promoter was developed. transgenic events were initially screened via selection media containing basta, followed by pcr and basta leaf paint assay after hardening. out of 138 successfully regenerated to events, only 12 had c ...201728667571
purification and functional properties of a novel glucoamylase activated by manganese and lead produced by aspergillus japonicus.microbial amylases are used to produce ethanol, glucose and can be applied in textiles products, detergents and other industries. this study aimed to determine the best carbon source concentration to induce the amylase production by a. japonicus, and its purification and biochemical characterization. for that, this fungus was cultivated in khanna medium, ph 5.5, for 4 days, at 25°c, in static condition, supplemented with potato starch and maltose in different concentrations. the fungal crude enz ...201728412339
effects of surfactants and microwave-assisted pretreatment of orange peel on extracellular enzymes production by aspergillus japonicus pj01.the effects of surfactants and microwave pretreatment of orange peel powder (opp) on the production of pectinase, cellulase, and xylanase by aspergillus japonicus pj01 in submerged fermentation were investigated. the results showed that when opp was pretreated only by microwave (630 w, 9 min, and liquid/solid ratio 5), the activities of exo-pectinase, carboxymethyl cellulase (cmcase), xylanase, and filter paper cellulase (fpase) were increased by 11.8, 20.6, 16.2, and 24.0 %, respectively, and w ...201525920331
production of the functional trisaccharide 1-kestose from cane sugar molasses using aspergillus japonicus β-fructofuranosidase.we report the production of the functional trisaccharide 1-kestose, o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside, by β-fructofuranosidase from aspergillus japonicus using sugar cane molasses as substrate. sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. the tetrasaccharide nystose, o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside, was produced from 1-kestose. cane sugar molasses mixed with w ...201727803952
saccharification of orange peel wastes with crude enzymes from new isolated aspergillus japonicus pj01.this study investigated the saccharification of orange peel wastes with crude enzymes from aspergillus japonicus pj01. pretreated orange peel powder was hydrolyzed by submerged fermentation (smf) and solid-state fermentation (ssf) crude enzymes, the results showed that 4 % (w/v) of solid loading, undiluted crude enzymes, and 45 °c were suitable saccharification conditions. the hydrolysis kinetics showed that the apparent michaelis-menten constant [formula: see text] and maximal reaction rate [fo ...201626718204
secondary metabolites from aspergillus japonicus cam231, an endophytic fungus associated with garcinia preussii.chemical investigation of aspergillus japonicus cam231, isolated from the leaf of garcina preussii collected in cameroon, yielded two new compounds; one pyrone derivative, hydroxy neovasinin (1) and one phenol derivative, asperolan (2), together with two known compounds neovasifurarone b (3) and variecolin (4). the structures of the two new compounds were established using intensive nmr spectroscopy and hrms spectra in comparison with data found in literature. the structure of compound 1 was con ...201727806646
structural characterization of eash (aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.aj_eash is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. the three dimensional structure of aj_eash (2.2 å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.201627885368
analyses of black aspergillus species of peanut and maize for ochratoxins and fumonisins.the genus aspergillus section nigri, or the black aspergilli, represents genetically closely related species that produce the mycotoxins, ochratoxins and the fumonisins. fumonisin b1 (fb1) is of an added concern because it is also a virulence factor for maize. our preliminary data indicated that black aspergilli could develop asymptomatic infections with maize and peanuts plants. symptomless infections are potential problems, because under favorable conditions, there is a potential for accumulat ...201424780336
semirational directed evolution of loop regions in aspergillus japonicus β-fructofuranosidase for improved fructooligosaccharide production.the aspergillus japonicus β-fructofuranosidase catalyzes the industrially important biotransformation of sucrose to fructooligosaccharides. operating at high substrate loading and temperatures between 50 and 60°c, the enzyme activity is negatively influenced by glucose product inhibition and thermal instability. to address these limitations, the solvent-exposed loop regions of the β-fructofuranosidase were engineered using a combined crystal structure- and evolutionary-guided approach. this semi ...201526253664
comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with aspergillus japonicus pj01.submerged fermentation (smf) and solid-state fermentation (ssf) of aspergillus japonicus pj01 for multi-enzyme complexes (mec) production were comparatively studied. the results showed that orange peel and wheat bran were the best substrates for mec production in smf and ssf, respectively. after 72 h of cultivation under smf, the maximal pectinase, cmcase, and xylanase activities reached 2610, 85, and 335 u/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under ssf, ...201526260269
the important ergot alkaloid intermediate chanoclavine-i produced in the yeast saccharomyces cerevisiae by the combined action of easc and ease from aspergillus japonicus.ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. these compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. biosynthesis of ergot alkaloids goes via the common intermediate chanoclavine-i, and studies of the key enzymes, ease and easc, involved in chanoclavine-i formation, have relied on gene compleme ...201425112180
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