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[riboflavin transport in cells of riboflavin-dependent yeast mutants].riboflavin was transported at a high rate into yeast cells of pichia guilliermondii and schwanniomyces occidentalis mutants capable of growth in a medium containing low concentrations of riboflavin, and having multiple susceptibility to some antibiotics and antimetabolites. sucrose and sodium azide inhibited transport of riboflavin. other riboflavin dependent mutants of pichia guilliermondii, pichia ohmeri, torulopsis candida, and saccharomyces cerevisiae, also growing in media containing low co ...1977329070
[a flavinogenic mutant of the yeast pichia guilliermondii with impaired iron transport].a mutant of the yeast pichia guilliermondii was produced by means of uv; the mutant was capable of riboflavin overproduction in the presence of high concentrations of iron in the medium. the content of total and non-hemin iron and cytochrome c, and the activity of catalase, were lower in the cells of the mutant than in the parent cells, while the activity of riboflavin synthetase was higher. the content of iron in the cells increased when the mutant was cultivated on media with citric acid, side ...1976933879
[mechanism of retroinhibition in e regulation of flavinogenesis in yeasts of genus pichia].the kinetics of the synthesis of a riboflavin (rf) precursor, 2,6-dihydroxy-5-amino-4-ribitylaminopyrimidine (dharap), was studied using the washed cells of rf-deficient mutants of pichia guilliermondii r7g and pichia ohmeri r32 with blocked lumasine synthetase. rf inhibited the synthesis of dharap while cycloheximide in the absence of rf had no effect on this process. the data suggest that flavins regulate the biosynthesis of rf in p. guillier mondii and p. ohmeri b7 means of feed-back inhibiti ...19751160631
a case of disseminated infection with pichia ohmeri. 200011205641
pichia ohmeri prosthetic valve endocarditis and review of the literature.fungal prosthetic valve endocarditis (pve) is a serious complication of valve replacement surgery. we report the first case of documented pichia ohmeri pve in an immunocompetent man who was successfully treated with valve replacement and antifungal therapy with amphotericin b.200211928850
a case of central-venous-catheter-associated infection caused by pichia ohmeri. 200212009828
first report of urinary tract infection due to pichia ohmeri. 200212226699
kodamaea (pichia) ohmeri fungaemia in a premature neonate.kodamaea ohmeri is a yeast that rarely causes human infections. the first case of k. ohmeri fungaemia in a premature neonate is reported; it was successfully treated with liposomal amphotericin b. biochemical identification of the yeast was performed by vitek ii and api and was confirmed by rrna gene sequencing. k. ohmeri as a human pathogenic yeast is uncommon to hospitalized neonates and immunocompromised individuals.200616434718
candida hyderabadensis sp. nov., a novel ascomycetous yeast isolated from wine grapes.three ascomycetous yeast strains were isolated from decaying green wine grapes, collected from hyderabad city in india. two strains, ys9 and ys21, were identified as kodamaea ohmeri and candida fermentati, respectively. the third strain, ys12(t), differs from candida parapsilosis, candida orthopsilosis and candida metapsilosis, the nearest phylogenetic neighbours, by 1.6-1.9% with respect to the nucleotide sequence of the d1/d2 domain of the 26s rrna gene and by 1.4-9.2% with respect to the nucl ...200717253980
four new yeasts in the candida mesenterica clade associated with basidiocarp-feeding beetles.we isolated five yeasts related to candida mesenterica from the digestive tract, frass, and habitat of beetles in six families inhabiting basidiocarps. based on rdna sequence comparisons and phenotypic characters, the yeasts were identified as kodamaea ohmeri and four undescribed taxa. phylogenetic analysis of combined small and large subunit ribosomal dna sequences placed the five taxa in a statistically well supported clade with c. mesenterica, candida suecica and other yeast species known fro ...200516389968
an outbreak of pichia ohmeri infection in the paediatric intensive care unit: case reports and review of the literature.since the first report in 1998, 10 cases of pichia ohmeri infection have been reported in the literature. here we present two new cases of p. ohmeri infection in the paediatric age group. the first case was an 8-month-old male infant, who was admitted with fever, convulsions and altered consciousness. conservative therapy was started with a presumptive diagnosis of encephalitis. the patient failed to respond to the given treatments and died on the 21st day of hospitalisation. the second case was ...200515982209
yeast involved in fermentation of coffea arabica in east africa determined by genotyping and by direct denaturating gradient gel electrophoresis.samples of coffea arabica were collected during the different stages of the fermentation from two production sites in tanzania. the yeasts community was identified by genotyping using its-pcr and sequence analysis of the d1/d2 domain of the 26s rrna gene. for confirmation, denaturating gradient gel electrophoresis (dgge) of pcr-amplified 26s rrna gene was performed to detect yeast directly from coffee samples without cultivation. yeast counts were in the range 4.0 x 10(4) - 5.0 x 10(7) cfu/g wit ...200415164358
infections by the yeast kodomaea (pichia) ohmeri: two cases and literature review.a 14-year-old boy who was neutropenic following chemotherapy for leukemia developed fungemia caused by the yeast kodomaea ohmeri ( pichia ohmeri). the infection was cured by catheter removal and the use of fluconazole. a 74-year-old man who had undergone surgeries for a subcutaneous tumor developed polymicrobic cellulitis involving kodomaea ohmeri. despite surgical debridement and antibiotic therapy, the patient died of complications. including these 2 cases, there have been 10 kodomaea ohmeri i ...200414722784
pichia ohmeri fungemia associated with phlebitis: successful treatment with amphotericin b.we report a case of fungemia caused by the yeast-form fungus pichia ohmeriin a 59-year-old hospitalized patient. p. ohmeri was found in all of the patient's blood cultures collected on days 52, 57, 59, and 64 of his hospital stay. intermittent fever developed on the 52nd hospital day and persisted for about 10 days. the patient had previously received intensive antimicrobial therapy for a ventriculoperitoneal shunt infection and subsequent nosocomial pneumonia. although a central venous catheter ...200312673414
native valve endocarditis due to pichia ohmeri.candida species can cause clinical manifestations in various organs of the cardiovascular system, i.e., the pericardium, myocardium, and endocardium, with endocarditis being the best-known clinical entity. endocarditis is seen primarily in intravenous drug users and in individuals with damaged native valves, especially in congenital heart disease or rheumatic valvular diseases, and in prosthetic heart valves. the authors present a case of pichia ohmeri endocarditis in an intravenous drug user, w ...200212382036
[the fungicidal activity and paradoxical effect of caspofungin against yeast. influence of culture medium and incubation time].the effect of culture medium and incubation time on the fungicidal activity of caspofungin and on the frequency of paradoxical effect has been studied in 144 blood culture isolates (42, candida tropicalis, 32 candida glabrata, 21 candida krusei, 20 candida parapsilosis, 11 candida guilliermondii, 4 candida famata, 3 candida lusitaniae, 3 blastoschizomyces capitatus, 2 saccharomyces cerevisiae, 2 yarrowia lipolytica, 1 candida inconspicua, 1 candida lambica, 1 candida sake y 1 pichia ohmeri). the ...200718563217
[evaluation of a new method for antifungal drugs susceptibility testing to yeasts].we compared the etest with a broth microdilution method (fp panel), performed according to the national committee for modified clinical laboratory standards (nccls) document m27-p guidelines, for determining the mics of 81 clinical isolates of yeasts (7 candida albicans, 8 candida glabrata, 10 candida parapsilosis, 6 pichia anomala, 10 candida tropicalis, 4 candida guilliermondii, 4 candida krusei, 6 trichosporon cutaneum, 5 candida ciferrii, 3 candida famata, 4 candida norvegensis, 2 rhodotorul ...200010783576
[in vitro amphotericin b susceptibility of korean bloodstream yeast isolates assessed by the clsi broth microdilution method, etest, and minimum fungicidal concentration test].although amphotericin b (amb) has a wide spectrum of activity that encompasses the majority of yeast isolates, there have been recent reports suggesting that some yeast isolates exhibit decreased susceptibility to amb. however, in vitro amb susceptibility of yeast species isolates from blood cultures in korea has not been fully surveyed.200818971615
pichia ohmeri peritonitis in a patient on capd: response to treatment with amphotericin. 200010716592
kodamaea nitidulidarum, candida restingae and kodamaea anthophila, three new related yeast species from ephemeral flowers.three new yeast species were discovered during studies of yeasts associated with ephemeral flowers in brazil, australia and hawaii. their physiological and morphological similarity to kodamaea (pichia) ohmeri suggested a possible relationship to that species, which was confirmed by rdna sequencing. kodamaea nitidulidarum and candida restingae were found in cactus flowers and associated nitidulid beetles in sand dune ecosystems (restinga) of south-eastern brazil. over 350 strains of kodamaea anth ...199910028276
pichia ohmeri fungemia.a patient with a history of diabetes, coronary artery disease, stroke, previous renal transplantation, and multiple hospital admissions for recurrent pancreatitis was transferred to the hospital from a chronic care facility because of fever and severe epigastric discomfort. at the time of admission, she was receiving hyperalimentation through a central venous tpn catheter. multiple blood cultures obtained on the first and second hospital days yielded pure cultures of the yeast, pichia ohmeri. th ...19989572030
the incidence of killer activity and extracellular proteases in tropical yeast communities.the presence of killer and proteolytic yeasts was studied among 944 isolates representing 105 species from tropical yeast communities. we found 13 killer toxin producing species, with pichia kluyveri being the most frequent. other killer yeast isolates were candida apis, candida bombicola, candida fructus, candida krusei, candida sorbosa, hanseniaspora uvarum, issatchenkia occidentalis, kloeckera apis, kluyveromyces marxianus, pichia membranaefaciens, pichia ohmeri-like, and sporobolomyces roseu ...19979115090
clinical isolates of candida guilliermondii include candida fermentati.clinical isolates of candida guilliermondii that were investigated by isoenzyme and randomly amplified polymorphic dna analyses represented two distinct species. the two species were distinguished on the basis of delayed fermentation of galactose. the larger group of isolates was closely related to the anamorph c. guilliermondii atcc 6260t (t = type strain) and its teleomorph, yamadazyma (= pichia) guilliermondii atcc 46036t. the remaining group, whose members fermented galactose, was very simil ...19979103626
survey of bovine mycotic mastitis in dairy herds in the state of são paulo, brazil.the purpose of this study was to isolate fungi from the quarter milk of cow udders from several dairy herds and to identify the different genera and species involved in mastitis. a total of 2078 milk samples from normal, clinical and subclinical mastitis quarters from 22 dairy herds of 16 districts in the state of são paulo, brazil was utilized in this survey. two hundred and fifty one (12.07%) fungi were isolated from the samples. two hundred and eight of these (82.86%) were yeasts and 30 (11.9 ...19938159214
development of a transformation system for the yeast yamadazyma (pichia) ohmeri.this communication describes the development of genetic tools for the yeast yamadazyma ohmeri. nystatin enrichment proved highly effective for isolating various auxotrophic strains, which were classified by complementation analysis. biosynthetic genes encoding known biochemical functions were isolated by polymerase chain reaction, including yoleu2 and youra3 that were sequenced. using these homologous genes as selective markers, dna transformation was accomplished by electroporation. transformat ...19947725795
the phylogeny of yamadazyma ohmeri (etchells et bell) billon-grand based on the partial sequences of 18s and 26s ribosomal rnas: the proposal of kodamaea gen. nov. (saccharomycetaceae).two strains of yamadazyma ohmeri were examined for their 18s and 26s rrna partial base sequences. the two strains had identical or very similar partial base sequences to those of the type strain. all the three strains of y. ohmeri were phylogenetically separated from any other accepted species of the genera yamadazyma, debaryomyces, pichia, williopsis, metschnikowia, and clavispora. based on the sequence data obtained and the phenotypic characteristics, kodamaea gen. nov. was proposed for the sp ...19957613010
role of de novo protein synthesis in the interconversion of glucose transport systems in the yeast pichia ohmeri.glucose-repressed cells of the yeast pichia ohmeri igc 2879 transported glucose by facilitated diffusion. derepression led to the formation of a glucose/proton symport and the simultaneous reduction of the facilitated diffusion capacity by about 70%. cycloheximide prevented this interconversion indicating its dependence on de novo protein synthesis (proteosynthetic interconversion). in buffer with 2% glucose the glucose/proton symport suffered irreversible inactivation while the facilitated diff ...19873036221
in vitro determination of phagocytic indices of candida berkhout species by rat peritoneal macrophages.the aims of this investigation were to study and describe the behaviour of 13 different species of candida, as compared with c. albicans, by means of phagocytosis assays in vitro. tests were carried out with rat peritoneal macrophages in contact with quantified suspensions of live yeasts. phagocytic indices, candidacidal activity and filamentation rat were tested microscopically after 3 h incubation at 37 degrees c. the phagocytic indices obtained allowed us to separate the fungi into four group ...19852580238
yeast microflora isolated from brazilian cassava roots: taxonomical classification based on molecular identification.a rapid molecular identification technique was applied on microbial microflora isolated from brazilian cassava roots given a yeast profile presented in the samples analyzed. a total of 24 strain isolated from cassava were initially grouped and identified in five groups using restriction-fragment length polymorphism (rflps) of 5.8s-its rdna region. sequencing analysis of the domains d1 and d2 of the 26s rrna gene or 5.8s rrna-its region were used to identify different groups of yeasts. representa ...201019924478
[determination of riboflavin kinase activity in yeast].it is established that the main reason of the riboflavin kinase (rfk, ec 2.7.1.26) low specific activity in the cell-free extracts of the yeast pichia guillermondii wickerham atcc 9058 is the presence of alkaline phosphatase (ec 3.1.3.1), effectively destructing flaven mononucleotide. by chromatography of the cell-free extracts of p. guillermondii on deae-sephadex a-50, cm-sphadex c-50, cm-cellulose, sephadexes g-75 and g-100 rfk and alkaline phosphatase may be separated completely. any of these ...1975174262
the emerging life-threatening opportunistic fungal pathogen kodamaea ohmeri: optimal treatment and literature review.the yeast kodamaea ohmeri rarely causes life-threatening human infections. however, risk factors, laboratory diagnoses, and treatments for k. ohmeri infection have been limited, and the optimal therapy for k. ohmeri infection has not been identified.201021291847
genetic relatedness of commensal strains of candida albicans carried in the oral cavity of patients' dental prosthesis users in brazil.the aim of this study is to describe the degree of yeast-colonization in diabetic and hemodialysed-users of dental prostheses. individuals (306) were examined using an oral rinse technique in order to evaluate the incidence of yeast-carriage, and genotype of c. albicans. yeasts were isolated from 68.4% (91/133) individual's dental prostheses users. dental prostheses were found to be a significant factor for the yeast colonization (p < 0.05). overall, the intensity of carriage was higher in diabe ...200717906942
kodamaea ohmeri as an emerging pathogen: a case report and review of the literature.kodamaea (pichia) ohmeri is a yeast-like fungus that has recently emerged as an important etiologic agent of fungemia in immunocompromised patients. we report such a case in a premature neonate born at 29 weeks of gestation. prior to developing fungemia, she had two episodes of bacterial sepsis on day 13 and day 32 due to enterobacter cloacae and staphylococcus epidermidis, respectively. kodamaea ohmeri was repeatedly isolated from blood cultures and its identity was determined by phenotypic cha ...201121438792
[kodamaea ohmeri fungemia associated with surgery].we report a case of fungemia caused by kodamaea (pichia) ohmeri associated with surgery in a patient with a history of diabetes and chronic renal failure. kodamaea ohmeri is a yeast rarely involved in human infections. since the first report of a case of fungemia in 1998, only thirteen cases of k. ohmeri infection have been so far described in the medical literature.200717604437
multitrophic interaction facilitates parasite-host relationship between an invasive beetle and the honey bee.colony defense by honey bees, apis mellifera, is associated with stinging and mass attack, fueled by the release of alarm pheromones. thus, alarm pheromones are critically important to survival of honey bee colonies. here we report that in the parasitic relationship between the european honey bee and the small hive beetle, aethina tumida, the honey bee's alarm pheromones serve a negative function because they are potent attractants for the beetle. furthermore, we discovered that the beetles from ...200717483478
identification and in vitro screening of avian yeasts for use as probiotic.the objective of this study was to isolate and identify yeast strains from broilers excreta and to evaluate in vitro their potential for probiotic use in animal production. methods and results: nine yeast strains were isolated and presumptively pre-identified by biochemical assays. these isolates were grouped in six different molecular profiles using pcr-fingerprinting technique. each profile was identified by sequencing of the d1/d2 domains of the large subunit of the 26s rrna gene. these yeast ...201122047814
kodamaea ohmeri isolates from patients in a university hospital: identification, antifungal susceptibility, and pulsed-field gel electrophoresis analysis.data on clinical isolates of kodamaea (pichia) ohmeri, an emerging fungal pathogen, are scarce. over the past 5 years, we identified yeast isolates from nine patients with fungemia as k. ohmeri by using the api 20c system. here, we reanalyzed these isolates first by sequencing the internal transcribed spacer 2 (its2) regions and then by growing the isolates on chromagar candida medium and subjecting them to pulsed-field gel electrophoresis (pfge). based on their its2 sequences, six of the nine i ...200717251396
screening wild yeast strains for alcohol fermentation from various fruits.wild yeasts on the surface of various fruits including grapes were surveyed to obtain yeast strains suitable for fermenting a novel wine with higher alcohol content and supplemented with rice starch. we considered selected characteristics, such as tolerance to alcohol and osmotic pressure, capability of utilizing maltose, and starch hydrolysis. among 637 putative yeast isolates, 115 strains exhibiting better growth in yeast-peptone-dextrose broth containing 30% dextrose, 7% alcohol, or 2% maltos ...201122783070
pichia ohmeri fungemia in a hematologic patient: an emerging human pathogen. 200617065010
inhibition of clinical and environmental cryptococcus neoformans isolates by two brazilian killer yeasts.two killer yeast strains (hb55 and hb88) capable of inhibiting human pathogenic fungi were isolated from leaves of hibiscus rosa-sinensis in brazil. these isolates were identified by conventional methods and sequencing of the d1/d2 region of the 26s rdna as kodamaea ohmeri. they inhibited all cryptococcus neoformans (vars. neoformans, grubii and gattii) strains tested, including reference, clinical and environmental isolates. the killer phenotype was not cured by thermal treatment.200616598831
[onychomycosis by yeast not common in diabetics of a health center].mexican diabetic population frequently presents mycosis under foot hyperkeratosis; however, in another type of onychomycosis as the ones that is assumed candida albicans is the causal agent, it is unknown the frequency, the prevalence and if another candida species or other yeasts are found.201626482238
[activity of voriconazole against yeasts isolated from blood culture determined by two methods].the in vitro activity of voriconazole has been determined by two methods: the reference m27-a2 and the marketed sensititre yeastone microdilution colorimetric method. the agreement (+/-2 dilutions) and correlation between methods as well as the percentage of errors has been determined. a total of 144 yeasts (47 candida albicans, 52 c. parapsilosis, 13 c. tropicalis, 10 c. krusei, 9 c. glabrata, 2 c. guilliermondii, 1 c. colliculosa, 1 c. dubliniensis, 2 trichosporum asahii, 1 t. mucoide, 1 trich ...200516446790
kodamaea (pichia) ohmeri fungaemia complicating acute myeloid leukaemia in a patient with haemochromatosis. 200818038331
purification and characterization of extracellular phytase from a marine yeast kodamaea ohmeri bg3.the extracellular phytase in the supernatant of cell culture of the marine yeast kodamaea ohmeri bg3 was purified to homogeneity with a 7.2-fold increase in specific phytase activity as compared to that in the supernatant by ammonium sulfate fractionation, gel filtration chromatography (sephadextrade mark g-75), and anion-exchange chromatography (deae sepharose fast flow anion-exchange). according to the data from sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular mass of t ...200818040741
production of phytase by a marine yeast kodamaea ohmeri bg3 in an oats medium: optimization by response surface methodology.statistical experimental designs were applied for the optimization of phytase production by a marine yeast kodamaea ohmeri bg3 in a cost-effective oats medium. using plackett-burman design, oats, ammonium sulfate and initial ph were identified as significant factors and these factors were subsequently optimized using a central composite design (ccd). the optimum variables that supported maximum enzyme activity were oats 1.0%, ammonium sulfate 2.3%, glucose 2.0%, nacl 2.0% and initial ph 6.3. the ...200818180156
trapping of aethina tumida murray (coleoptera: nitidulidae) from apis mellifera l. (hymenoptera: apidae) colonies with an in-hive baited trap.the effectiveness of two lures for trapping the small hive beetle, aethina tumida, by means of in-hive traps was tested by field trials in apiaries located in florida, delaware, and pennsylvania during 2003-2005. both lures included a mixture (pollen dough) consisting of bee pollen and commercial pollen substitute formulated with or without glycerol and honey. before it was used in the traps, the dough was conditioned either by the feeding of adult small hive beetles or by inoculation with the y ...200718284723
pcr-denaturing gradient gel electrophoresis analysis of microbial community in soy-daddawa, a nigerian fermented soybean (glycine max (l.) merr.) condiment.soy-daddawa, a fermented soybean (glycine max (l.) merr.) condiment, plays a significant role in the culinary practice of west africa. it is essential to understand the microbial community of soy-daddawa for a successful starter culture application. this study investigated the microbial community structure of soy-daddawa samples collected from nigerian markets, by pcr-denaturing gradient gel electrophoresis (dgge) targeting the v3-v5 region of the 16s rrna gene of bacteria and internal transcrib ...201626796580
onychomycosis incidence in type 2 diabetes mellitus patients.the onychomycosis incidence was determined in 250 type 2 diabetes mellitus (t2dm) patients who were registered at the internal medicine service from a mexico city general hospital throughout a year (january-december 2006). out of the total of studied t2dm patients, 93 (37.2%) showed ungual dystrophy and from these, in 75.3% a fungal etiology was corroborated. out of 70 patients, 34 were men and 36 women, with an average of 63.5 years. correlation between t2dm evolution time and onychomycosis was ...200818373212
molecular cloning, characterization, and expression of the phytase gene from marine yeast kodamaea ohmeri bg3.the extracellular phytase structural gene was isolated from the cdna of the marine yeast, kodamaea ohmeri bg3, using the switching mechanism at 5'-end of rna transcript (smart)trade mark rapid-amplification of cdna ends (race) cdna amplification kit. the gene had an open reading frame of 1389 bp and the coding region of the gene had no intron. it encoded 462 amino acid residues of a protein with a putative signal peptide of 15 amino acids. the protein sequence deduced from the extracellular phyt ...200918672057
fluconazole-resistant kodamaea ohmeri fungemia associated with cellulitis: case report and review of the literature.kodamaea (pichia) ohmeri was formerly considered a contaminant, but is now known to be a significant human pathogen that has been shown to cause fungemia, endocarditis, funguria, and peritonitis in immunocompromised patients. we report a case of fungemia caused by k. ohmeri in a 71-year-old man with cellulitis. the patient was sent to the emergency room due to leg edema, fever, and change of consciousness. during hospitalization, a series of examinations including blood cultures were performed. ...200919411182
rare yeasts causing fungemia in immunocompromised and haematology patients: case series from delhi.systemic fungal infection related to fluconazole-resistant yeasts are emerging in immunocompromised patients. in this case-series, we report eight cases of fungemia caused by trichosporon spp. (2), stephanoascus ceferrii (1), kodamaea ohmeri (1), pichia kutrawersi (2), candida rugosa (1) and candida lusitianae (1) in immunocompromised patients. all the yeasts except (trichosporon asahii) were sequenced. as these rare species are inherently resistant to antifungal agents and they may lead to the ...201526470968
isolation, identification and growth determination of lactic acid-utilizing yeasts from the ruminal fluid of dairy cattle.ruminal organic acid production, especially lactic acid, can be modified by feeding cattle highly concentrated diets, which have been shown to adversely affect dairy cattle health. therefore, the use of lactic acid-utilizing organisms is considered to be a potential method for controlling lactic acid levels. this study was conducted to isolate and identify lactic acid-utilizing yeasts from the ruminal fluid of dairy cattle and to determine the specific growth rate and generation time when using ...201323565722
yeast diversity of ghanaian cocoa bean heap fermentations.the fermentation of the theobroma cacao beans, involving yeasts, lactic acid bacteria, and acetic acid bacteria, has a major influence on the quality of the resulting cocoa. an assessment of the microbial community of cocoa bean heap fermentations in ghana resulted in 91 yeast isolates. these were grouped by pcr-fingerprinting with the primer m13. representative isolates were identified using the d1/d2 region of the large subunit rrna gene, internal transcribed spacer sequences and partial actin ...200919473277
oral candidiasis caused by kodamaea ohmeri in a hiv patient in chennai, india.kodamaea ohmeri was isolated from a 38-year-old hiv seropositive woman with pseudomembranous oral candidiasis. the isolate was identified by the api 20 c yeast identification system and confirmed by sequence analysis. antifungal susceptibility testing done by e-test showed that the isolate was susceptible to voriconazole, amphotericin b and caspofungin.201019531098
kodamaea ohmeri infection in a neonate.kodamaea ohmeri is an extremely uncommon human pathogenic yeast. it causes opportunistic infection in immunocompromised hosts. we report a case of kodamaea ohmeri fungemia in a preterm neonate who succumbed despite antifungal therapy.200919638663
purification of candida guilliermondii and pichia ohmeri killer toxin as an active agent against penicillium expansum.an antifungal assay with cell-free culture supernatant of pichia ohmeri 158 and candida guilliermondii p3 was tested against penicillium expansum strain #2 at 25 degrees c by measuring hyphal length and percentage conidia germination. c. guilliermondii was more effective against p. expansum conidia germination (58.15% inhibition), while p. ohmeri showed higher inhibition of mycelial growth (66.17%), indicating a probable mechanism associated with killer activity. this killer toxin (molecular mas ...200919680874
production of d-arabitol by a newly isolated kodamaea ohmeri.a new yeast, isolated from natural osmophilic sources, produces d-arabitol as the main metabolic product from glucose. according to 18s rrna analysis, the nh-9 strain belongs to the genus kodamaea. the optimal culture conditions for inducing production of d-arabitol were 37 degrees c, neutral ph, 220 rpm shaking, and 5% inoculum. the yeast produced 81.2 +/- 0.67 g l(-1) d-arabitol from 200 g l(-1) d-glucose in 72 h with a yield of 0.406 g g(-1) glucose and volumetric productivity q(p) of 1.128 g ...201019768471
candidaemia and antifungal susceptibility testing in a teaching hospital.we reviewed cases of candidaemia at universiti kebangsaan malaysia medical centre from 1st january 2005 to 30th june 2006. all blood cultures positive for candida species or its teleomorphs within the study period were identified and antifungal susceptibility testing was performed. out of 50 blood isolates, 20 (40%) were identified as candida albicans, 16 (32%) c. tropicalis, five (10%) c. parapsilosis, three (6%) c. famata, two (4%) c. glabrata, two (4%) pichia ohmeri, one (2%) c. krusei and on ...200919852325
kodamaea (pichia) ohmeri fungemia in a pediatric patient admitted in a public hospital.kodamaea (pichia) ohmeri is a yeast species that has not been reported to be a frequent cause of human infections. the current report describes a case of fungemia caused by k. ohmeri in a 3-year-old female patient hospitalized in the public hospital maria alice fernandes, natal, rn, brazil. the patient had previously received antimicrobial therapy due to a peritoneal infection and nosocomial pneumonia, and had a central venous catheter implanted. kodamaea ohmeri was isolated from blood and the t ...200919888811
molecular identification of yeasts associated with traditional egyptian dairy products.this study aimed to examine the diversity and ecology of yeasts associated with traditional egyptian dairy products employing molecular techniques in yeast identification. a total of 120 samples of fresh and stored domiati cheese, kariesh cheese, and "matared" cream were collected from local markets and examined. forty yeast isolates were cultured from these samples and identified using the restriction-fragment length polymorphism (rflps) of 5.8s-its rdna region and sequencing of the domains d1 ...200919895478
kodamaea ohmeri fungaemia successfully treated with caspofungin. 201019896340
uncommon fungi isolated from diabetic patients toenails with or without visible onychomycoses.kodamaea ohmeri and prototheca wickerhamii are rare pathogens for humans, and even more rare as cause of onychomycosis. this work reports the second case of onychomycosis by k. ohmeri and the fourth of onycoprotothecosis; it was made in public health institutions in the hidalgo state, mexico, studying 261 diabetic patients during 2005 and 2006. kodamaea ohmeri was isolated from toenails of a 51-year-old female patient, and p. wickerhamii from three female patients of 48, 49, and 61 years old, re ...201121512855
kodamaea ohmeri tricuspid valve endocarditis with right ventricular inflow obstruction in a neonate with structurally normal heart.the yeast kodamaea (pichia) ohmeri is a rare human pathogen with infrequent report of neonatal infection. native valve endocarditis by kodamaea ohmeri is extremely rare. the current case report describes a case of fatal nosocomial native valve endocarditis without any structural heart defects in a 40dayold baby. the patient was referred to our institute after having icu stay of 18 days in another hospital for necrotizing enterocolitis and was found to have obstructive tricuspid valve mass and fu ...201121677814
monitoring aethina tumida (coleoptera: nitidulidae) with baited bottom board traps: occurrence and seasonal abundance in honey bee colonies in kenya.the population dynamics of the honey bee pest aethina tumida murray (small hive beetle) have been studied in the united states with flight and langstroth hive bottom board traps baited with pollen dough inoculated with a yeast kodamaea ohmeri associated with the beetle. however, little is known about the population dynamics of the beetle in its native host range. similarly baited langstroth hive bottom board traps were used to monitor the occurrence and seasonal abundance of the beetle in honey ...201022182536
escmid and ecmm joint clinical guidelines for the diagnosis and management of rare invasive yeast infections.the mortality associated with invasive fungal infections remains high with that involving rare yeast pathogens other than candida being no exception. this is in part due to the severe underlying conditions typically predisposing patients to these healthcare-related infections (most often severe neutropenia in patients with haematological malignancies), and in part due to the often challenging intrinsic susceptibility pattern of the pathogens that potentially leads to delayed appropriate antifung ...201424102785
matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the rapid identification of yeasts causing bloodstream infections.few studies have systematically standardised and evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) for identification of yeasts from bloodstream infections. this is rapidly becoming pertinent for early identification of yeasts and appropriate antifungal therapy. we used 354 yeast strains identified by polymerase chain reaction (pcr) sequencing for standardisation and 367 blind clinical strains for validation of our maldi-tof ms protocols. we al ...201525658527
[kodamaea ohmeri: fungemia in a patient with invasive bladder cancer]. 201323608203
[clinical features and homological analysis of pichia ohmeri-caused hospital-acquired fungemia in premature infants].to analyze the clinical features of fungemia caused by pichia ohmeri (p. ohmeri) in neonate intensive care unit and explore its molecular biological characteristics so as to improve its diagnosis and treatment level.201323578509
[invasive infection due to kodamaea ohmeri]. 201223044085
fungal peritonitis in patients undergoing peritoneal dialysis (pd) in brazil: molecular identification, biofilm production and antifungal susceptibility of the agents.this paper presents data on fungal peritonitis (fp) in patients undergoing peritoneal dialysis (pd) at the university hospital of botucatu medical school, são paulo, brazil. in a total of 422 patients, 30 developed fp, from which the medical records and the fungal isolates of 23 patient cases were studied. all patients presented abdominal pain, cloudy peritoneal effluent, needed hospitalization, had the catheter removed and were treated with fluconazole or fluconazole plus 5-flucitosine; six of ...201627143636
candida species epidemiology 2000-2013: a laboratory-based report.to describe a prospective laboratory-based surveillance of candida species that were collected from different anatomical sites of patients admitted to the university of malaya medical centre, malaysia, from the year 2000 to 2013.201526216479
identification and antifungal susceptibility of a large collection of yeast strains isolated in tunisian hospitals.in this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) was used as a rapid method to identify yeasts isolated from patients in tunisian hospitals. when identification could not be exstablished with this procedure, sequencing of the internal transcribed spacer with 5.8s ribosomal dna (rdna) (its1-5.8s-its2) and d1/d2 domain of large-subunit (lsu rdna) were employed as a molecular approach for species differentiation. candida albicans was the dom ...201323768242
asymptomatic oral yeast carriage and antifungal susceptibility profile of hiv-infected patients in kunming, yunnan province of china.oral candida colonization and its relation with predisposing factors in hiv-infected patients have received wide concerns during recent decades. in this study, we investigated asymptomatic oral candida carriage rate, species distribution and antifungal susceptibility of 604 hiv-infected patients and 851 healthy individuals in kunming, yunnan province of china.201323356471
photodynamic inactivation of biofilms formed by candida spp., trichosporon mucoides, and kodamaea ohmeri by cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29h, 31h-phthalocyanine (znpc).the biofilms formed by opportunistic yeasts serve as a persistent reservoir of infection and impair the treatment of fungal diseases. the aim of this study was to evaluate photodynamic inactivation (pdi) of biofilms formed by candida spp. and the emerging pathogens trichosporon mucoides and kodamaea ohmeri by a cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29h,31h-phthalocyanine (znpc). biofilms formed by yeasts after 48 h in the bottom of 96-well microtiter plates were treated wi ...201222278349
identification of yeasts isolated from raffia wine (raphia hookeri) produced in côte d'ivoire and genotyping of saccharomyces cerevisiae strains by pcr inter-delta.raffia wine is a traditional alcoholic beverage produced in several african countries where it plays a significant role in traditional customs and population diet. alcoholic fermentation of this beverage is ensured by a complex natural yeast flora which plays a decisive role in the quality of the final product. this present study aims to evaluate the distribution and the diversity of the yeast strains isolated in raffia wine from four sampling areas (abengourou, alépé, grand-lahou and adzopé) in ...201627339306
epidemiological study of a large cluster of fungaemia cases due to kodamaea ohmeri in an indian tertiary care centre.while performing molecular confirmation of phenotypically identified candida tropicalis isolates, we re-identified a few isolates as kodamaea ohmeri. this led us to the present epidemiological investigation of k. ohmeri fungaemia cases. all phenotypically identified c. tropicalis blood isolates during october 2008 through to december 2009 at our advanced paediatric centre were included for molecular identification by sequencing of the internal transcribed spacer and d1/d2 regions of rdna. after ...201424004250
clinical characteristics and risk factors of non-candida fungaemia.the incidence of fungaemia has been increasing worldwide. it is important to distinguish non-candida fungaemia from candidaemia because of their different antifungal susceptibilities. the aims of this study were to investigate the clinical characteristics of non-candida fungaemia and identify the clinical factors that differentiate it from candidaemia.201323714136
occurrence and diversity of yeasts in the mangrove ecosystems in fujian, guangdong and hainan provinces of china.mangrove wetland is a unique ecosystem and has rich bioresources. in this article, the roots, stems, branches, leaves, barks, fruits, and flowers from 12 species of the mangrove plants and six species of the accompanying mangrove plants, seawater and sediments in mangrove ecosystems in china were used as sources for isolation of yeasts. a total of 269 yeasts strains were obtained from the samples. the results of routine identification and phylogenetic analysis showed that they belonged to 22 gen ...201223997323
halotolerance and survival kinetics of lactic acid bacteria isolated from jalapeño pepper (capsicum annuum l.) fermentation.the microbiota associated with spontaneous fermentation of vegetables in a saline substrate may represent an important group of bacteria in the food industry. in this work, the lactic acid bacteria (lab) weissella cibaria, lactobacillus plantarum, lactobacillus paraplantarum, and leuconostoc citreum were identified by partial 16s rrna gene sequence analysis. in addition, entophytic bacteria such as pantoea eucalypti, pantoea anthophila, enterobacter cowanii, and enterobacter asburiae were detect ...201425039289
catheter-related bloodstream infection caused by kodamaea ohmeri: a case report and literature review.kodamaea ohmeri is a rare yeast pathogen that has recently emerged as an important cause of fungemia in immunocompromised patients. however, appropriate therapy for this infection remains unclear. we report a case of catheter-related blood stream infection caused by k. ohmeri in a 58-year-old patient who improved after removal of the central venous catheter and administration of micafungin. considering the antibiotic susceptibility of this pathogen and reviewing literature, echinocandins may be ...201728188094
a rare life-threatening kodamaea ohmeri endocarditis associated with hemophagocytic lymphohistiocytosis. 201728153549
fungemia due to kodamaea ohmeri in a young infant and review of the literature.fungal infections have become an important cause of morbidity and mortality in hospitalized children due to many complicating and underlying conditions. we present the case of a newborn infant with fungemia due to kodamaea ohmeri who had a good outcome of the infection after using the combination of antifungal treatment and central venous catheter removal.201627630816
[kodamaea ohmeri infection in a newborn with a mediastinal mass].invasive fungal infections are a considerable cause of morbidity, mortality, increased hospital stay durations, and high health care costs, during neonatal period. in this period, the premature infants are the most affected. candida species are the leading cause of invasive fungal infections. the majority of neonatal candida infections are caused by c. albicans, c. parapsilosis, c. glabrata and c. tropicalis, although other fungus species are being reported. one such emerging pathogen is k. ohme ...201627606654
fungemia due to rare opportunistic yeasts: data from a population-based surveillance in spain.fungemia due to rare yeasts constitutes an emerging but poorly investigated condition. data on risk factors, clinical features, therapy, and outcome of episodes of fungemia due to rare (non-candida, non-cryptococcus) yeasts were analyzed in a population-based surveillance program conducted in 29 spanish hospitals between may 2010 and april 2011. species identification (dna sequencing) and antifungal susceptibility testing (eucast and clsi methods) were centrally performed. fourteen out of 767 ep ...201727495321
boron and coumaphos residues in hive materials following treatments for the control of aethina tumida murray.in the search of alternatives for controlling aethina tumida murray, we recently proposed the baa trap which uses boric acid and an attractant which mimics the process of fermentation caused by kodamaea ohmeri in the hive. this yeast is excreted in the feces of a. tumida causing the fermentation of pollen and honey of infested hives and releasing compounds that function as aggregation pheromones to a. tumida. since the boron is the toxic element in boric acid, the aim of this article is to asses ...201627092938
azole resistance in candida spp. isolated from catú lake, ceará, brazil: an efflux-pump-mediated mechanism.since, there is no study reporting the mechanism of azole resistance among yeasts isolated from aquatic environments; the present study aims to investigate the occurrence of antifungal resistance among yeasts isolated from an aquatic environment, and assess the efflux-pump activity of the azole-resistant strains to better understand the mechanism of resistance for this group of drugs. for this purpose, monthly water and sediment samples were collected from catú lake, ceará, brazil, from march 20 ...201626887224
[a case of bioprosthetic valve endocarditis due to pichia ohmeri]. 201626813558
kodamaea ohmeri fungemia in an immunocompetent patient treated with micafungin: case report and review of the literature.kodamaea (pichia) ohmeri is an unusual yeast-form fungus that has recently been identified as an important etiology of fungemia, endocarditis, cellulitis, funguria and peritonitis in immunocompromised patients. we report a case of k. ohmeri fungemia in a 34-year-old hospitalized patient with thrombophlebitis. the patient was admitted to the hospital for evaluation and management of an acquired tracheo-esophageal fistula secondary to an impacted denture. fever developed on hospital day 22, and ph ...201020449770
identification of yeasts during alcoholic fermentation of tchapalo, a traditional sorghum beer from côte d'ivoire.this study investigated the diversity and dynamics of yeasts involved in alcoholic fermentation of a traditional sorghum beer from côte d'ivoire, tchapalo. a total of 240 yeast strains were isolated from fermenting sorghum wort inoculated with dry yeast from two geographic regions of côte d'ivoire (abidjan and bondoukou). initial molecular identification to the species level was carried out using rflp of pcr-amplified internal transcribed spacers of rdna (its1-5.8s-its2). ten different profiles ...201121318423
characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol.this study attempted to identify oleaginous yeasts and selected the strain that accumulated the largest quantity of lipid for lipid production from glycerol. two-step screening of 387 yeast strains revealed 23 oleaginous strains that accumulated quantities of lipid higher than 20 % of their biomass when cultivated in glycerol. these strains were identified to be four ascomycetous yeast species i.e. candida silvae, kodamaea ohmeri, meyerozyma caribbica, and pichia manshurica, and five basidiomyce ...201526615742
fungemia due to kodamaea ohmeri: first isolating in italy. case report and review of literature.kodamaea ohmeri, also known as pichia ohmeri, is a yeast belonging to the saccharomycetes family. in 2012, our hospital has recorded the first case of fungemia caused by k. ohmeri in an 80-year-old male, admitted to intensive care following an acute anterior-lateral myocardial infarction. k. ohmeri grew in blood cultures. biochemical identification was confirmed using matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry and molecular sequencing. antifungal sus ...201526404421
infection control challenges of infrequent and rare fungal pathogens: lessons from disseminated fusarium and kodamaea ohmeri infections. 201525998614
kodamaea ohmeri isolate from two immunocompromised patients: first report in italy.kodamaea ohmeri is an unusual yeast-form fungus that has recently been identified as an important aetiological agent of fungaemia, endocarditis, cellulitis, funguria and peritonitis in immunocompromised patients. we present two new isolated of k. ohmeri. the microorganisms were identified by chromagar candida medium, vitekii system and api id32c. biochemical identification of the two yeast isolates was confirmed by sequence analysis of the 26s ribosomal dna. antifungal susceptibility testing don ...201322882303
oral colonization by yeasts in hiv-positive patients in brazil.in hiv-infected patients, colonization of the oral cavity by potential pathogenic yeast may lead to development of systemic fungemia. we evaluated the prevalence of yeast in the oral cavity of brazilian hiv-positive patients and verified whether or not the species characterized were enzymatically active. furthermore, the species identified were tested for their susceptibility to antifungal treatment.201322370749
fungemia due to rare opportunistic yeasts: data from a population-based surveillance in spain.fungemia due to rare yeasts constitutes an emerging but poorly investigated condition. data on risk factors, clinical features, therapy, and outcome of episodes of fungemia due to rare (non-candida, non-cryptococcus) yeasts were analyzed in a population-based surveillance program conducted in 29 spanish hospitals between may 2010 and april 2011. species identification (dna sequencing) and antifungal susceptibility testing (eucast and clsi methods) were centrally performed. fourteen out of 767 ep ...201628339535
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