| immunization of white pekin ducklings against pasteurella anatipestifer infection. | since pasteurella anatipestifer (pa) serotypes 1, 2, and 5 gave no significant protection against heterologous serotype challenge, a trivalent formalin-inactivated bacterin containing all these serotype cells was developed and tested in the laboratory. two inoculations of bacterin in white pekin ducklings at 10 and 17 days of age gave highly significant protection against challenge with virulent organisms. the protection lasted only 2 weeks after the second inoculation. a third bacterin inoculat ... | 1979 | 526203 |
| laboratory and field trials on a formalin inactivated vaccine for the control of pasteurella anatipestifer septicaemia in ducks. | a series of laboratory trials on a number of different types of inactivated pasteurella anatipestifer vaccines suggested that a formolised vaccine would be the most suitable for protection. a satisfactory dose of vaccine was found to be approximately 3 x 109 cells administered intramuscularly. the efficacy of this vaccine was confirmed by a field trial on a farm where anatipestifer septicaemia was endemic, ducks being vaccinated at three weeks of age before their transfer to pasture from indoor ... | 1979 | 542721 |
| pasteurella anatipestifer infection in turkeys. | in a case of excessive mortality in seven-week-old turkeys, the primary lesion at necropsy was severe fibrinous pericarditis and epicarditis. after 48 hours of incubation at 37 c, pasteurella anatipestifer was isolated. the infection responded to antibiotic therapy with a combination of neomycin and oxytetracycline in the drinking water. the lesions suggested chlamydial infection, but cultural procedures for chlamydia were negative. | 1977 | 606228 |
| spondylitis in turkeys associated with experimental pasteurella anatipestifer infection. | nine previously vaccinated turkeys were inoculated intravenously with pasteurella anatipestifer, and blood samples were taken periodically to evaluate the potential of chronically infected turkeys to serve as reservoirs of infection for blood-feeding arthropod vectors. vertebral osteomyelitis (spondylitis), as yet unreported in the literature in association with infection with the organism, was found in the thoracic vertebrae of five out of nine inoculated turkeys, and p. anatipestifer was isola ... | 1992 | 1627101 |
| a retrospective analysis on the epizootiological aspects of outbreaks of pasteurella anatipestifer infection in turkeys in minnesota. | the possible risk factors associated with outbreaks of pasteurella anatipestifer infection in turkeys were analyzed to study the epizootiology of the disease. the data for the study was obtained from flocks affected and unaffected with pasteurella infection. the results of the analysis suggested that overcrowding, preexisting viral infections, increased activity of wild birds on the farm, and shorter down time were possible factors that initiate the occurrence of the disease in turkey flocks. th ... | 1991 | 1924089 |
| [pasteurella multocida as the cause of disease outbreaks in commercial poultry flocks]. | six cases of fowl cholera in growing turkeys and 3 in adult breeder chickens of the broiler type as well as one case each of a pasteurella (p.) multocida-associated disease in ducklings and goslings were described in consideration of own laboratory findings and available informations of the case history. furthermore a report is given on a treatment strategy successfully used in turkeys with highly acute fowl cholera. all the p. multocida strains isolated culturally could be assigned to the subsp ... | 1991 | 1953629 |
| mycoplasmas and acholeplasmas isolated from ducks and their possible association with pasteurellas. | two hundred and sixty-three cases of clinically diseased ducks of all ages were examined for the presence of mycoplasmas. mycoplasmas and acholeplasmas belonging to more than eight serogroups were cultured from 68 of them, and comprised 12 m anatis, one m columbinasale, two m gallinaceum, two m gallinarum, nine m synoviae, three unidentified mycoplasma species, 37 acholeplasma laidlawii and one unclassified acholeplasma belonging to each of serogroups 7 and 8. they were identified by biochemical ... | 1990 | 2399638 |
| pasteurella anatipestifer infections in california turkey flocks: circumstantial evidence of a mosquito vector. | outbreaks of pasteurella anatipestifer infections in california turkey flocks were investigated and found to have a seasonal distribution, with a peak incidence in fall, coinciding with peak culex mosquito populations. an experiment was conducted to test the hypothesis that mosquitoes may serve as vectors for p. anatipestifer infections in turkeys. four 7-week-old turkey poults were exposed for 7 days to mosquitoes captured from turkey barns during a field outbreak of p. anatipestifer serotype 1 ... | 1989 | 2619669 |
| laboratory assessment of protection given by experimental pasteurella anatipestifer vaccine. | an experimental, inactivated p. anatipestifer type 'g' vaccine was produced containing 10(9) cfu/0.5 ml and aluminium hydroxide adjuvant (25% final concentration). from comparative pathogenicity studies of field isolates type 'a', 'd' and 'g', the 'g' serotype was selected as being most virulent and suitable for challenge. vaccination trials, in the laboratory, showed that a single subcutaneous inoculation (0.5 ml) of the above vaccine at 14 days provided the best protection against challenge up ... | 1989 | 2790442 |
| [pasteurella anatipestifer sp.i.c. in pekin ducks: pathogenicity tests and immunization with an inactivated, homologous, monovalent (serotype 6/b) oil emulsion vaccine]. | | 1988 | 3293968 |
| [pasteurella anatipestifer infection of waterfowl]. | | 1987 | 3319489 |
| [pasteurella anatipestifer infection of water fowl]. | | 1987 | 3322779 |
| influence of the route of infection of pasteurella anatipestifer on the clinical and immune responses of white pekin ducks. | the clinical, pathological and immunological responses were compared in ducklings infected by the intramuscular, oral and intranasal routes with virulent pasteurella anatipestifer. intramuscular challenge resulted in clinical signs of infection and caused 100 per cent mortality within three days. no disease signs or death were observed in the orally challenged ducks. whereas intranasal inoculation caused no deaths, signs of infection were observed in two of 12 birds four days later. in the orall ... | 1988 | 3387674 |
| pasteurella anatipestifer infection in commercial meat-type turkeys in california. | a disease outbreak characterized by respiratory signs, occasional neurologic signs, and increased mortality in commercial meat turkeys from four separate companies in central california was investigated in the late summer and early fall of 1986. the disease syndrome affected turkeys from 6 to 15 weeks of age and caused a severe fibrinous pericarditis, perihepatitis, and airsacculitis. bacteriologic and serologic examinations as well as virus- and chlamydia-isolation attempts initially failed to ... | 1987 | 3442544 |
| taxonomy of pasteurella anatipestifer. 1. dna base composition and dna-dna hybridization analysis. | dna was isolated from 15 strains of pasteurella anatipestifer and from one strain each of moraxella nonliquefaciens, m. bovis, pasteurella multocida, p. haemolytica, p. gallinarum, p. pneumotropica, and p. ureae. the guanine-plus-cytosine contents of p. anatipestifer ranged from 32 to 35 mole %, whereas those of moraxella and pasteurella spp. were much higher, ranging from 40 to 45 mole %. dna-dna hybridization analysis revealed that homology of nine p. anatipestifer strains to strains atcc 1184 ... | 1987 | 3579792 |
| taxonomy of pasteurella anatipestifer. 2. cellular fatty-acid profile by gas chromatography. | methylated cellular fatty acids of representative strains of pasteurella spp., moraxella spp., and p. anatipestifer were subjected to gas chromatography in an attempt to further support the independence of p. anatipestifer from both pasteurella and moraxella. all pasteurella spp. and moraxella spp. revealed group characteristics specific for each genus that could be easily differentiated from the unique profile of p. anatipestifer. all p. anatipestifer strains tested showed similar fatty-acid pr ... | 1987 | 3579793 |
| an outbreak of moraxella (pasteurella) anatipestifer infection in ducklings in japan. | | 1987 | 3682537 |
| phenotypic and genetic relationships of so-called moraxella (pasteurella) anatipestifer to the flavobacterium/cytophaga group. | so-called moraxella (or pasteurella) anatipestifer and members of the flavobacterium/cytophaga group exhibit remarkable common features: lack of flagellation, low guanine + cytosine content of the chromosomal dna, production of menaquinones and branched-chain fatty acids, absence of carbohydrate fermentation, and similar patterns of hydrolytic enzymes. using the renaturation method of dna:dna hybridization two urease-negative european isolates and the urease-positive type strain (which was isola ... | 1986 | 3739211 |
| a numerical taxonomic study of actinobacillus, pasteurella and yersinia. | a numerical taxonomic study of strains of actinobacillus, pasteurella and yersinia, with some allied bacteria, showed 23 reasonably distinct groups. these fell into three major areas. area a contained species of actinobacillus and pasteurella: a. suis, a. equuli, a. lignieresii, p. haemolytica biovar a, p. haemolytica biovar t, p. multocida, a. actinomycetemcomitans, 'p. bettii', 'a. seminis', p. ureae and p. aerogenes. also included in a was a composite group of pasteurella pneumotropica and p. ... | 1985 | 3840837 |
| laboratory and field trials with formalin-inactivated escherichia coli (o78)-pasteurella anatipestifer bacterin in white pekin ducks. | a combination escherichia coli serotype o78 and pasteurella anatipestifer bacterin was developed and tested in white pekin ducks in laboratory and field trials. inoculations with bacterin at 2 and 3 weeks of age provided significant protection against challenge with virulent e. coli o78 and pasteurella anatipestifer serotypes 1, 2, and 5. no significant cross-protection was observed against heterologous e. coli serotypes, although there was a slight reduction in mortality in ducklings challenged ... | 1985 | 3885930 |
| pasteurella anatipestifer infection in migrating whistling swans. | | 1974 | 4436930 |
| suspected pasteurella anatipestifer infection in ducklings in new south wales. | | 1972 | 4640757 |
| pasteurella anatipestifer infection in fowls in australia. | | 1973 | 4689817 |
| pasteurella anatipestifer as a cause of mortality in semiwild pen-raised mallard ducks in south carolina. | | 1973 | 4766569 |
| pasteurella anatipestifer infection in geese. | | 1973 | 4766572 |
| isolation of pasteurella anatipestifer from black swan (cygnus atratus). | | 1970 | 4917083 |
| pasteurella anatipestifer infection in ducks in australia. | | 1972 | 5075211 |
| pasteurella anatipestifer infection in pheasants. a case report. | | 1970 | 5464754 |
| case report. pasteurella anatipestifer in turkeys. | | 1970 | 5466099 |
| [pasteurella anatipestifer infection in ducklings]. | | 1970 | 5528530 |
| central-nervous-system lesions of spontaneous pasteurella anatipestifer infection in ducklings. | | 1969 | 5813247 |
| pasteurella (pfeifferella) anatipestifer serotypes isolated from cases of anatipestifer septicemia in ducks. | | 1969 | 5816777 |
| protection of ducklings with a broth-grown pasteurella anatipestifer bacterin. | pasteurella anatipestifer (pa) serotypes 1, 2, and 5 grew to high densities in tryptic soy broth and tryptose broth (tb) when the media were continuously shaken or aerated. growth in 100 ml to 15 liters of tb exceeded an absorbance of 1.0 at a wavelength of 525 nm (about 0.7 for a 1/3 dilution) and contained more than 10(10) colony-forming units per ml. a bacterin was prepared from the three serotypes of pa grown in aerated tb. two subcutaneous injections of this bacterin protected 70% to 85% of ... | 1984 | 6487192 |
| serologic types and physiologic characteristics of 46 avian pasteurella anatipestifer cultures. | forty-six strains of pasteurella anatipestifer isolated from different avian species were examined to determine their serologic types and physiologic characteristics. serologic types were determined by a gel-diffusion precipitin test. antigens from 39 field isolates reacted with antisera prepared from seven p. anatipestifer reference strains representing serotypes 1, 2, 4, 6, and 7. antigens from five isolates did not react and could not be typed with available reagents. gel precipitin reactions ... | 1982 | 6818940 |
| rofenaid in the control of pasteurella anatipestifer and escherichia coli infections in ducklings. | in a series of controlled battery trials, rofenaid (at doses ranging from 0.02 to 0.12% active drug in feed) was administered to ducklings infected experimentally with pasteurella anatipestifer and escherichia coli strains. against p. anatipestifer, rofenaid at 0.02 to 0.12% levels in feed either prevented or reduced mortality, gross lesions, and bacterial isolations. furthermore, the weight gain and feed conversion of infected rofenaid-medicated ducklings were superior to those of uninfected un ... | 1980 | 7002146 |
| studies of pasteurella anatipestifer: an approach to its classification. | moraxella spp., pasteurella spp., and strains of p. anatipestifer were tested for biochemical reactions, growth temperature, viability, antibacterial sensitivity, and dna base composition. p. anatipestifer was viable for shorter periods at 37 c, showed high resistance to polymyxin b and kanamycin, and had lower base composition than reported for moraxella and pasteurella spp. because of these conditions, p. anatipestifer should be excluded from the genera pasteurella and moraxella. | 1981 | 7259676 |
| serotypes of pasteurella anatipestifer isolated from commercial white pekin ducks in the united states. | pasteurella anatipestifer isolates from commercially raised, clinically diseased white pekin ducks were serotyped by agglutination and agar-gel precipitin methods. plate agglutination was found to be the most suitable test for preliminary classification. based on national animal disease center (ames, iowa) nomenclature, six serotypes were classified as types 1, 2, 3, 5, 7, and 8. these were compared with strains held by houghton poultry research station (houghton, huntingdon, england), at presen ... | 1981 | 7259684 |
| effect of chemotherapeutic agents on pasteurella anatipestifer infection in white pekin ducklings. | in a series of experiments, various chemotherapeutic agents administered in feed were evaluated for their efficacy against experimental pasteurella anatipestifer (pa) infection in white pekin ducklings. the feeding of medicated diets was started 3 days prior to challenge and continued throughout each experiment. novobiocin and lincomycin, when fed at adequate concentrations, were the most effective medicaments tested. mortality in treated groups was 0-18% compared with 45-92% mortality in contro ... | 1980 | 7393839 |
| [on the taxonomy of actinobacillus, haemophilus, and pasteurella: dna base composition, respiratory quinones, and biochemical reactions of representative collection cultures (author's transl)]. | in a comparative study, 63 collection cultures representing 38 nomenspecies of, or assigned to, the genera actinobacillus, haemophilus, or pasteurella were characterized by phenotypical features and deoxyribonucleic acid base composition. the latter was calculated from the thermal denaturation point. biochemical reactions were tested in differential media commonly used for enterobacteriaceae, and two test procedures were compared: (i) pure cultures with haematin and nicotine adenine dinucleotide ... | 1980 | 7424243 |
| serological survey of infections in waterfowl in the guadalquivir marshes (spain). | a serological survey was performed in the guadalquivir marshes (southern spain) in order to determine the presence and diffusion of six infective agents in wild waterfowl. the analysis covered 712 waterfowl from 13 species belonging to five taxa (podicipediformes, ciconiiformes, anseriformes, gruiformes, and charadriiformes). a range of immunological techniques led to detection of antibodies against the six infective agents studied: chlamydia psittaci (13.3%), mycoplasma anatis (3.5%), pasteurel ... | 1994 | 7980291 |
| ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract. | the phylogenetic position and various genotypic, chemotaxonomic, and classical phenotypic characteristics of 21 gram-negative avian isolates were studied. these strains constitute a genotypically homogeneous taxon in rrna superfamily v, as shown by dna-rrna hybridization data. determination of the 16s rrna sequence of this taxon revealed its detailed position within the "flavobacter" subgroup of the "flavobacter-bacteroides" phylum as described by gherna and woese (r. gherna and c. r. woese, sys ... | 1994 | 8123560 |
| physiological characteristics, antimicrobial susceptibility and serotypes of pasteurella anatipestifer isolated from ducks in thailand. | twenty isolates of pasteurella (moraxella) anatipestifer from ducks with serositis and septicemia in thailand between 1988 and 1989 were characterized by various tests. eighteen isolates fermented glucose and maltose, 3 fructose and 1 each mannose, arabinose, trehalose or sorbitol. all isolates produced gelatinase but not urease, while 2, 3, 5 and 6 produced indole, were camp positive, and were proteolytic for milk and coagulated serum respectively. seven enzymes, phosphatase alkaline, esterase ... | 1994 | 8203123 |
| influence of newcastle disease virus on the severity of pasteurella anatipestifer infection in turkeys. | this study was designed to examine whether vaccine or virulent strains of newcastle disease virus (ndv) would potentiate the disease caused by pasteurella anatipestifer infection in turkeys. the studies were conducted in turkeys of two age groups. there were three experiments. in two experiments four-week-old turkeys were exposed either to vaccine or virulent strains of ndv after experimental p anatipestifer infection. in the third experiment 14-week-old turkeys were first exposed to virulent nd ... | 1993 | 8235089 |
| riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the flavobacterium-cytophaga rrna homology group. | the phylogenetic position of the causative agent of septicemia anserum exsudativa, now most often referred to as [moraxella] anatipestifer (brackets indicate a generically misnamed taxon) or "[pasteurella] anatipestifer," was established by performing rrna cistron similarity studies. [moraxella] anatipestifer belongs to rrna superfamily v, together with the genera flavobacterium, cytophaga, flexibacter, weeksella, capnocytophaga, and sphingobacterium. the detailed structure of rrna superfamily v ... | 1993 | 8240957 |
| pasteurella anatipestifer-like bacteria associated with respiratory disease in pigeons. | two cases of respiratory disease in pigeons are described. the first case involved pneumonia and tracheitis, and the second case involved tracheitis. in both cases, unusual gram-negative, non-fermenting, short rod-shaped bacteria were recovered along with other microorganisms. the bacteria produced small, glistening, gray colonies on blood agar, did not grow on macconkey agar, were unreactive on several biochemical tests, and resembled pasteurella anatipestifer. neither pigeon isolate was distin ... | 1993 | 8257393 |
| [pasteurella anatipestifer: a controllable farm problem]. | the performance of 13 flocks of ducks on a duck farm decreased markedly. post-mortem and bacteriological examinations indicated that pasteurella anatipestifer was a major cause, although salmonella spp., escherichia coli and treponema spp. were also detected. use of an autovaccine against pasteurella anatipestifer markedly reduced the signs and symptoms in the second part of fattening period. | 1993 | 8367840 |
| riemerella anatipestifer infection of domestic ducklings. | | 1997 | 9028598 |
| phylogenetic position of riemerella anatipestifer based on 16s rrna gene sequences. | riemerella anatipestifer, the causative agent of septicemia anserum exsudativa (also called new duckling disease), belongs to the family flavobacteriaceae of gram-negative bacteria. we determined the dna sequences of the rrs genes encoding the 16s rrnas of four r. anatipestifer strains by directly sequencing pcr-amplified rrs genes. a sequence similarity analysis confirmed the phylogenetic position of r. anatipestifer in the family flavobacteriaceae in rrna superfamily v and allowed fine mapping ... | 1997 | 9103649 |
| dna fingerprinting of riemerella anatipestifer. | seventeen restriction endonucleases were evaluated for use in dna fingerprinting of riemerella anatipestifer. digestion of chromosomal dna with either hinfi or ddei restriction endonuclease, followed by submarine electrophoresis in agarose and staining with ethidium bromide, resulted in dna fingerprint profiles that could be easily resolved. hinfi produced readable fingerprint patterns in the 2.7-20-kb range and was used to distinguish dna fingerprint profiles among 89 strains of r. anatipestife ... | 1998 | 9533086 |
| detection of acid production from carbohydrates by riemerella anatipestifer and related organisms using the buffered single substrate test. | one hundred and twenty-one riemerella anatipestifer field strains from wild birds, domesticated poultry and pigs were examined for their ability to produce acid from carbohydrates by using conventional biochemical and buffered single substrate (bss) test methods. the type strains of the species r. anatipestifer and taxometrically related genera chryseobacterium and bergeyella were included in the study. in contrast to 10 indole-positive r. anatipestifer variant strains, only a few of the 111 typ ... | 1998 | 9646458 |
| coenonia anatina gen. nov., sp. nov., a novel bacterium associated with respiratory disease in ducks and geese. | taxon 1502 was originally described as a riemerella anatipestifer-like bacterium causing exudative septicaemia in ducks and geese. in the present study, an integrated genotypic and phenotypic approach was used to elucidate the phylogenetic affiliation and taxonomic relationships of 12 strains of taxon 1502. whole-cell protein and fatty acid analyses and an extensive biochemical examination by using conventional tests and several api microtest systems indicated that all isolates formed a homogene ... | 1999 | 10319512 |
| molecular fingerprinting of riemerella anatipestifer by repetitive sequence pcr. | riemerella anatipestifer is a gram-negative rod-shaped bacterium associated with epizootic infections in poultry. a total of 35 r. anatipestifer isolates including the type strain atcc11845t, reference and field strains for 18 different serotypes were characterized by repetitive sequence based-pcr (rep-pcr) with outwardly-directed primers based on the repetitive extragenic palindromic (rep) consensus sequence. this technique was applied by using either extracted genomic dna or preparation of who ... | 1999 | 10418875 |
| identification of a virulence-associated protein homolog gene and isra1 in a plasmid of riemerella anatipestifer. | riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. we have previously reported that a 3.9-kb plasmid, pcfc1, carries protein genes (vapd1 and vapd2) that are similar to virulence-associated genes of other bacteria. in the present study, we report the complete sequence of a second plasmid of 5.6 kb, pcfc2. pcfc2 has a 28% g-c content and three large open reading frames (orfs). one of the orfs (designated asvapd1) encodes a polypeptide that shares 53.9, 53.9, 48.3 ... | 1999 | 10481080 |
| characterization of a predominant immunogenic outer membrane protein of riemerella anatipestifer. | the ompa gene, encoding the 42-kda major antigenic outer membrane protein ompa of riemerella anatipestifer, the etiololgical agent of septicemia anserum exsudativa, was cloned and expressed in escherichia coli. recombinant ompa displayed a molecular mass similar to that predicted from the nucleotide sequence of the ompa gene but lower than that observed in total cell lysates of r. anatipestifer. the ompa gene showed a conserved c-terminal region comprising the ompa-like domain and a variable n-t ... | 2000 | 10702488 |
| duck immune responses to riemerella anatipestifer vaccines. | riemerella anatipestifer (ra) infection is probably the most economically important infectious disease of farm ducks worldwide but the immune responses to natural infection and vaccines are poorly understood. we have used the lymphocyte transformation test (ltt) to study the expression of cell-mediated immunity (cmi), and the enzyme-linked immunosorbent assay to monitor antibody (ab) production following administration of formalin-inactivated and live attenuated serotype 2 (= g) ra vaccines. lym ... | 2000 | 10717285 |
| exclusion of strain 670/89 as type strain for serovar 20 of riemerella anatipestifer. | classical phenotypic characterisation and numeric analysis of whole-cell fatty acid patterns of twenty-one type strains of hitherto reported riemerella anatipestifer serovars revealed that the type strain of serovar 20 does not belong to the species riemerella anatipestifer sensu stricto and, therefore, it has to be excluded as a representative riemerella anatipestifer serotype strain. | 2000 | 10726364 |
| pathogenicity of a low-virulence duck virus enteritis isolate with apparent immunosuppressive ability. | duck enteritis virus (dev) was isolated from commercial 2-to-6-wk-old white pekin ducks experiencing 25%-30% mortality and high morbidity. secondary infections with pasteurella multocida, riemerella anatipestifer, and escherichia coli were frequently seen in affected ducks. the isolated virus was identical to the prototype dev by virus neutralization test but differed from the classic dev by causing lymphoid organ atrophy and inconsistent hemorrhagic lesions in the intestinal annular bands. atte ... | 2000 | 11007006 |
| an outbreak of duck viral enteritis (duck plague) in domestic muscovy ducks (cairina moschata domesticus) in illinois. | duck viral enteritis (dve) was diagnosed in an outbreak of the disease in a resident population of muscovy ducks (cairina moschata domesticus) on a privately owned multispecies game bird production facility in illinois, where it claimed 625 ducks. this disease condition had not been reported previously in domestic ducks in illinois. although other varieties and age groups of domestic waterfowl (i.e., black ducks, rhumen ducks, pekin ducks, ducklings, and geese) were present on the game bird farm ... | 2001 | 11417839 |
| studies on the prevalence of riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains. | a total of 44 bacterial strains obtained from 49 clinically healthy ducklings of different ages originating from four different farms were identified as members of the species riemerella anatipestifer (ra) using conventional biochemical test methods. numerical analysis of the whole-cell fatty acid patterns of these isolates resulted in two different clusters, one of which showed a similar pattern to that of the type strain of ra. strains having a different fatty acid methyl esters (fame)-profile ... | 2001 | 11666036 |
| vaccination of ducks with recombinant outer membrane protein (ompa) and a 41 kda partial protein (p45n') of riemerella anatipestifer. | the generation of protective immunity against riemerella anatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (gst) fusion's proteins of ompa, a 42kda major outer membrane protein, and p45n', a 41kda n-terminal fragment of a newly identified 45kda potential surface protein from r. anatipestifer. the dna encoding ompa and p45n' were isolated from r. anatipestifer serotype 15 (field strain 110/89) and serotype 19 (reference strain 30/90) ... | 2002 | 11731174 |
| identification and characterization of camp cohemolysin as a potential virulence factor of riemerella anatipestifer. | riemerella anatipestifer is responsible for exudative septicemia in ducks. the genetic determinant of the camp cohemolysin, cam, from a strain of r. anatipestifer was cloned and expressed in escherichia coli. chromosomal dna from serotype 19 strain 30/90 was used to construct a gene library in pbluescript ii sk(-) vector in e. coli xl-1-blue strain. the clones containing recombinant plasmids were screened for the camp reaction with staphylococcus aureus. those that showed cohemolysis were chosen ... | 2002 | 11889100 |
| development of an elisa using a recombinant 41 kda partial protein (p45n') for the detection of riemerella anatipestifer infections in ducks. | riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. an indirect enzyme-linked immunosorbent assay (elisa) has been developed to facilitate early detection of r. anatipestifer infection in ducks. the antigen used was a recombinant 41 kda n-terminal fragment (rp45n') of a newly characterized r. anatipestifer potential surface protein, p45, which was expressed in escherichia coli as an n- ... | 2002 | 12220809 |
| a potential new serotype of riemerella anatipestifer isolated from ducks in thailand. | eighty isolates of riemerella anatipestifer representing 71 outbreaks of riemerellosis in thailand between 1994 and 1999 were serotyped using the gel diffusion precipitin test. based on the precipitation patterns, 25 serological profiles containing one to three antigenic determinants were recognized. heat-stable antigens of the organism reacted with antisera raised against 16 known serotypes and an untypable strain 698/95. the most prevalent serotype appeared to be serotype 7, followed by seroty ... | 2002 | 12396349 |
| antimicrobial susceptibility of riemerella anatipestifer isolated from ducks and the efficacy of ceftiofur treatment. | the in vitro susceptibilities of 50 field isolates of riemerella anatipestifer from ducks to ceftiofur and 16 other commonly used antimicrobials were determined. the mic90 values (mic refers to minimum inhibitory concentrations) for the antimicrobials used in this study are as follows: penicillin was 16 microg/ml; ceftiofur was 32 microg/ml; cephalothin, chloramphenicol, flumequine, and kanamycin were 64 microg/ml; nalidixic acid, nitrofurantoin, and sulfamethoxazole were 128 microg/ml; amikacin ... | 2003 | 12580291 |
| development and application of an elisa for the detection of duck antibodies against riemerella anatipestifer antigens in egg yolk of vaccinees and in serum of their offspring. | a direct and an indirect antibody enzyme-linked immunosorbent assay for duck yolk igy and duck serum igy was developed and tested on egg yolk and serum of ducks vaccinated with riemerella anatipestifer (ra). tests were performed either with primary antibodies labelled with horseradish peroxidase or with alkaline phosphatase-labelled secondary antibodies reacting with specifically bound rabbit anti-duck igy antibodies, respectively. ra-specific igys in egg yolk from three ducks increased rapidly ... | 2003 | 12675899 |
| a strain of moraxella anatipestifer (pfeifferella anatipestifer) isolated from ducks. | | 1954 | 13200095 |
| histobacteriology of the genus pasteurella. i. pasteurella anatipestifer. | | 1961 | 13767219 |
| cloning, expression, and purification of the virulence-associated protein d from xylella fastidiosa. | in this study, an efficient expression system, based on the pet32xa/lic vector, for producing a xylella fastidiosa virulence-associated protein d, found to have a strong similarity to riemerella anatipestifer and actinobacillus actinomycetencomitans vapd protein, is presented. the protein has a molecular mass of 17.637 da and a calculated pi of 5.49. the selected xfa0052 gene was cloned in the pet32xa/lic vector and the plasmid was transformed into escherichia coli bl21 (de3) strain at 37 degree ... | 2004 | 15358353 |
| genetic variation of the ompa and 16s rrna genes of riemerella anatipestifer. | the genetic diversity of the 16s rrna and ompa genes of riemerella anatipestifer was investigated. a 16s rrna gene-based pcr was able to amplify all 18 taiwanese strains and 10 reference strains. the identity of 16s rrna sequence of these strains and seven other sequences retrieved from genbank was 95.0-100.0%. the percentage identity of the ompa sequence of the 15 taiwanese strains and eight reference strains amplified in this study and two other sequences retrieved from genbank was 88.1-100.0% ... | 2005 | 15763739 |
| the effect of route of inoculation and challenge dosage on riemerella anatipestifer infection in pekin ducks (anas platyrhynchos). | riemerella anatipestifer is a gram-negative bacteria that can cause disease in a wide variety of wild and domesticated birds, especially waterfowl. the infection can be peracute, acute, or chronic. although various routes of transmission have been proposed, to date, there is little information on the effects of route of transmission and challenge dosage on r. anatipestifer infection. hence, the objective of this study was to determine the effect of route of inoculation and challenge dosage on r. ... | 2005 | 15839421 |
| pasteurella anatipestifer as a cause of mortality in captive wild waterfowl. | an outbreak of pasteurella anatipestifer infection in young wild waterfowl at the niska waterfowl research center resulted in losses of approximately 100 blue and snow geese, one white-fronted goose, five mandarin ducks, one black duck and one wood duck. clinical signs included diarrhea, paralysis and tremors. gross lesions were fibrin deposits on serosal surfaces, hemorrhages on the epicardium, consolidation of the lungs, cloudy or flaky deposits on the air sacs, and dark, swollen spleens. micr ... | 1970 | 16512147 |
| effects of anti-bursin monoclonal antibody on immunosuppression in the duck (cherry valley duck). | to study the immunologic function of bursin, we analyzed the effects of anti-bursin monoclonal antibody (mab) on the immunosuppression in ducks (cherry valley duck) by injecting various doses of the anti-bursin mab into 13-d duck embryos. after hatch, cell-mediated immune activity and humoral responses were studied using lymphocyte proliferation test, tube agglutination test, and indirect enzyme-linked immunosorbent assay to detect anti-escherichia coli antibodies and antibodies to riemerella an ... | 2006 | 16523625 |
| [rapid identification of riemerella anatipestifer on the basis of specific pcr amplifying 16s rdna]. | riemerella anatipestifer (ra) infection is the main disease causing severe losses in duck production. because ra is characterized more by the absence than by the presence of specific phenotypic properties and different scholar had the different results of biochemical detection, it can't always be identified quickly and correctly only by the phenotypic properties or biochemical characteristics. the research object was to develop a species specific pcr method for ra detection. because of the conse ... | 2006 | 16579457 |
| dna fingerprinting of riemerella anatipestifer isolates from ducks. | | 2007 | 17209094 |
| development of a novel pcr assay specific for riemerella anatipestifer. | riemerella anatipestifer is a significant pathogen of waterfowl and turkeys. due to their similar ecology and morphological and cultural characteristics it is important to differentiate r. anatipestifer infections from those caused by pasteurella multocida. present study describes a novel pcr assay that is capable of rapid and species-specific identification of r. anatipestifer from bacterial cultures. | 2007 | 17257252 |
| genetic analysis of a duck circovirus detected in commercial pekin ducks in new york. | the genetic organization of the duck circovirus (ducv) 33753-52 detected in commercial pekin duck flocks from long island, ny, is described. the nucleotide sequence of virus 33753-52 exhibited high similarity with ducvs previously detected in germany and hungary. it is possible that this ducv from new york shares the same ancestor with the european counterparts. the virus 33753-52 exhibited genetic features characteristic of other circoviruses, such as the presence of two major open reading fram ... | 2007 | 17461272 |
| dose response study of enrofloxacin against riemerella anatipestifer septicaemia in muscovy and pekin ducklings. | the efficacy of enrofloxacin against riemerella anatipestifer (formerly pasteurella anatipestifer) septicaemia of muscovy and pekin ducklings was assessed in an artificial challenge model which reproduced typical duck riemerellosis with very high mortality. mortality, clinical signs, gross lesions, microbiological clearance, feed intake and body weight gain were the efficacy criteria studied. a pulsing medication in drinking water for 4 h at 25, 50 or 100 ppm enrofloxacin on the first day, follo ... | 1997 | 18483945 |
| phenotypic characteristics of riemerella anatipestifer and similar micro-organisms from various hosts. | a total of 199 riemerella anatipestifer (ra) and ra-like field strains isolated culturally from birds of 12 different species and from pigs were characterized using classical phenotypic and chemotaxonomic tests. the ra reference strain atcc 11845 was included in the study. on the basis of the classical phenotypic characteristics studied and the numerical analysis of the whole-cell fatty acid patterns, the ra reference strain and 123 field isolates were assigned to the indole negative (in) varian ... | 1998 | 18483963 |
| molecular characterization of a plasmid isolated from riemerella anatipestifer. | sixty strains of riemerella anatipestifer were isolated from ducks and geese with infectious serositis in taiwan. sixty per cent of the isolates (36/60) contained a 3.9 b plasmid, 12% (7/60) contained 6.5 b and 16 b plasmids, 5% (3/60) contained 2.9, 16 and 18 b plasmids and 13% contained no plasmid (14/60). the 3.9 b plasmid (designated as pcfc1) was completely sequenced to determine if it encoded virulence factors. pcfc1 was 27% g-c and had four large open reading frames (orf). two of the orfs ... | 1998 | 18484011 |
| genomic diversity and molecular differentiation of riemerella anatipestifer associated with eight outbreaks in five farms. | riemerella anatipestifer causes infectious serositis of ducks and geese. the genomic diversity of r. anatipestifer associated with outbreaks in waterfowls was studied using 24 multidrug-resistant r. anatipestifer isolates collected from the visceral organs of ducks and geese from seven outbreaks in four goose farms and one outbreak in one duck farm. seven methods were used to differentiate these isolates. plasmid patterns differed in plasmid number and size, ranging from 2.9 kb to 20 kb, and pro ... | 2008 | 18568653 |
| [analysis of outer membrane proteins of riemerella antipestifer]. | an isolated virulence riemerella anatipestifer strain passaged 200 times on tsb agar were used for the virulent to avirulent conversion. the effects of passage on biological properties of outer membrane proteins (omps) were investigated using the virulent and avirulent strains. transmission electron microscopy demonstrated that the avirulent strain produced lower amounts of outer membrane vesicles and the outer membrane decreased, the cytoplasmic appearance jumbled. the omps of the virulent stra ... | 2008 | 18616167 |
| new serotypes of riemerella anatipestifer isolated from ducks in thailand. | thirty-two riemerella anatipestifer isolates from ducks were serotyped by agar gel precepitin test using chicken antisera against serotypes 1 to 19 r. anatipestifer reference strains. the heat stable saline extracts from 29 field isolates reacted with the antisera of serotypes 1, 6, 7, 10, 11, 14, or 19. the isolates belonging to serotype 1 were the most prevalent (56.3%). antigens from the remaining three isolates did not react with any of the available antisera. additional investigations showe ... | 1995 | 18645776 |
| salpingitis in web-footed birds: prevalence, aetiology and significance. | among 4,893,447 ducks slaughtered during a 10 year period in a single slaughterhouse in denmark 7,190 (0.15%) were condemned because of salpingitis. a further 21,658 ducks (0.44%) were approved for human consumption after the inflamed salpinx had been removed. annual condemnation due to salpingitis varied from 0 to 0.53% and total condemnation varied from 1.30 to 2.72%, with an average of 1.98%. sixteen different bacterial species were isolated in pure culture from salpingitis in 7 week-old duck ... | 1995 | 18645801 |
| immunogenicity of riemerella anatipestifer broth culture bacterin and cell-free culture filtrate in ducks. | strains belonging to riemerella anatipestifer serotype 1 are responsible for most of the major disease outbreaks caused by this organism in ducks in thailand. therefore, the immunogenicity induced by a broth culture bacterin prepared from a local strain (1081) of this serotype was studied in ducks and a protective index (pi) was derived. the inactivated vaccine contained the equivalent of 5 x 10(9) colony-forming units of bacteria per 0.5-ml dose. a single subcutaneous inoculation of the broth c ... | 1996 | 18645893 |
| isolation of moraxella anatipestifer from embryonated goose eggs. | twenty six embryonated goose eggs which had not hatched at term were investigated bacteriologically. moraxella anatipestifer was isolated from seven of them. this suggests that vertical transmission is possible and might explain the problems of eradicating moraxella infection by hygienic means only. | 1989 | 18679866 |
| muscovy ducks as a source of pasteurella anatipestifer infection in turkey flocks. | an outbreak of disease, characterised by neurological signs and increased mortality, occurred in 17 out of 50 turkey farms in a village. virological tests were negative as were routine bacteriological tests of the organs. however, pasteurella anatipestifer was repeatedly isolated from the brain. the source of the infection was probably a group of muscovy ducks. | 1990 | 18679922 |
| immunogenicity and safety of a live pasteurella anatipestifer vaccine in white pekin ducklings: laboratory and field trials. | oral or aerosol administration of a live trivalent pasteurella anatipestifer (pa) vaccine (sero-types 1, 2 and 5) gave significant protection against homologous challenges in white pekin ducklings. vaccination of one-day-old ducklings by either method provided protection for at least 42 days of age (last challenge). the vaccine did not cause any untoward signs or mortality in one-day-old ducklings exposed to ten times the recommended dose. the vaccine strains did not revert to virulence on 10 ba ... | 1991 | 18680038 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 18706944 |
| an investigation of duck circovirus and co-infection in cherry valley ducks in shandong province, china. | the co-infection of duck circovirus (ducv) with riemerella anatipestifer (ra) or/and escherichia coli (e. coli) or/and duck hepatitis virus i (dhv-i) in cherry valley ducks in china's shandong province was investigated by using polymerase-chain-reaction (pcr)-based methods. for this study, 742 ducks sampled at random from 70 duck farms during 2006-2007 were examined using pcr and dot-blot hybridisation (dbh) tests. overall the ducv infection rate was 33.29%. compared with those at 2 weeks of age ... | 2009 | 18760549 |
| development of an enzyme-linked immunosorbent assay for the detection of humoral antibody to pasteurella anatipestifer. | an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to pasteurella anatipestifer in duck sera is described. as part of the initial assay development, micro-titration plates from different manufacturers were assayed for their suitability to bind p. anatipestifer antigen. the nunc immunopiate ii was chosen, on account of its overall reproducibility (5.5% coefficient of variation) and the absence of an edge effect. optimum concentrations of reagents were determined and the ... | 1987 | 18766597 |
| antigenic studies on pasteurella anatipestifer, species incertae sedis, using slide and tube agglutination. | comparative serological investigations including all available serotypes of pasteurella anatipestifer, species incertae sedis, have shown that six american serotypes designated by arabic numbers were covered already by previously described serotypes. two out of 12 existing english serotypes designated alphabetically were shown to be identical. it is suggested that arabic numbers are used instead of letters for sero-type designation. consequently, two new serotypes isolated in denmark are designa ... | 1982 | 18770200 |
| development and evaluation of an antigen-capture elisa for detection of the ul24 antigen of the duck enteritis virus, based on a polyclonal antibody against the ul24 expression protein. | an antigen-capture enzyme-linked immunosorbent assay (ac-elisa) method was developed for the efficient detection of the ul24 antigen of the duck enteritis virus (dev) using polyclonal antibodies. ducks and rabbits were immunized, respectively, with expressed ul24 recombinant protein. the igg antibodies against ul24 from ducks and rabbits were purified and used as the capture antibodies. the specificity of the optimized ac-elisa was evaluated by use of dev, duck hepatitis virus (dhv), duck hepati ... | 2009 | 19467266 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 19475729 |
| phylogenetic relationships of riemerella anatipestifer serovars and related taxa and an evaluation of specific pcr tests reported for r. anatipestifer. | the aims of the present investigation were to characterize and identify serovars of riemerella anatipestifer and riemerella-like isolates genetically and to test the specificity of pcr tests reported for the identification of r. anatipestifer. | 2010 | 19840176 |
| identification of riemerella anatipestifer genes differentially expressed in infected duck livers by the selective capture of transcribed sequences technique. | riemerella anatipestifer is the causative agent of duck septicaemia. determination of r. anatipestifer virulence mechanisms will help us to effectively control this contagious agent. the differentially expressed gene profile of r. anatipestifer in infected duck livers was therefore identified and compared with in vitro cultures by selective capture of transcribed sequences analysis. a total of 48 genes were identified, of which 43 were genes that encode enzymes for amino acid biosynthesis and me ... | 2009 | 19937518 |
| pathogenesis of riemerella anatipestifer in turkeys after experimental mono-infection via respiratory routes or dual infection together with the avian metapneumovirus. | riemerella anatipestifer (ra) is the causative agent of septicaemic and exudative diseases in a variety of bird species. despite numerous outbreaks, little is known about the pathogenicity of ra for turkeys. we investigated the development of ra-induced disease in commercial turkey poults following ra inoculation via different respiratory routes. inoculation by aerosol or injection into the abdominal air sac led to systemic infection and mild gross lesions, including pericarditis, epicarditis an ... | 2009 | 19937539 |
| antibiotic susceptibility of riemerella anatipestifer field isolates. | kirby-bauer tests were used to analyze the antibiotic resistance of 224 isolates of riemerella anatipestifer isolated between 1998 and 2005. among the 36 antibiotics tested, 50% of the analyzed isolates were resistant to ampicillin, ceftazidime, aztreonam, cefazolin, cefepime, cefuroxime, oxacillin, penicillin g, rifampin, and trimethoprim/sulfamethoxazole. higher levels of resistance were detected for aztreonam, cefepime, oxacillin, penicillin g, ceftazidime, and trimethoprim/sulfamethoxazole ( ... | 2009 | 20095163 |
| antimicrobial peptides of an anti-lipopolysaccharide factor, epinecidin-1, and hepcidin reduce the lethality of riemerella anatipestifer sepsis in ducks. | antimicrobial peptides (amps) are effective against a wide range of microbes, but still no research results have reported their use in duck disease therapy. riemerella anatipestifer (ra) is a gram-negative bacterium which infects ducks and causes very significant economic losses. the minimum inhibitory concentrations (mics) of epinecidin-1 for the tested ra strains ranged 6.25-50microg/ml, those of the salf55-76 cyclic peptide ranged 12.5-25microg/ml, those of the salf55-76 linear peptide ranged ... | 2010 | 20138098 |
| characterization of biofilm formation by riemerella anatipestifer. | riemerella anatipestifer (ra) causes epizootics of infectious disease in poultry and results in serious economic losses, especially for the duck industry. the present study focuses on understanding the biofilm-producing ability of ra strains in attempt to explain the intriguing persistence of ra post-infection on duck farms. four ra serotype reference strains and 39 field ra isolates were measured for the biofilm formation by crystal violet staining. eighteen out of the 43 ra strains produced bi ... | 2010 | 20226600 |
| a thymidine kinase recombinant protein-based elisa for detecting antibodies to duck plague virus. | duck plague virus (dpv) is the causative agent of duck plague (dp) that causes significant morbidity and mortality throughout duck-producing areas of the world. the diagnosis of dp currently relies on the use of live or inactivated whole dpv virion as antigens in elisa, but it is too laborious and expensive for routine application, and it is still difficult to get purified dpv virion with current technology. | 2010 | 20416075 |