| ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract. | the phylogenetic position and various genotypic, chemotaxonomic, and classical phenotypic characteristics of 21 gram-negative avian isolates were studied. these strains constitute a genotypically homogeneous taxon in rrna superfamily v, as shown by dna-rrna hybridization data. determination of the 16s rrna sequence of this taxon revealed its detailed position within the "flavobacter" subgroup of the "flavobacter-bacteroides" phylum as described by gherna and woese (r. gherna and c. r. woese, sys ... | 1994 | 8123560 |
| riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the flavobacterium-cytophaga rrna homology group. | the phylogenetic position of the causative agent of septicemia anserum exsudativa, now most often referred to as [moraxella] anatipestifer (brackets indicate a generically misnamed taxon) or "[pasteurella] anatipestifer," was established by performing rrna cistron similarity studies. [moraxella] anatipestifer belongs to rrna superfamily v, together with the genera flavobacterium, cytophaga, flexibacter, weeksella, capnocytophaga, and sphingobacterium. the detailed structure of rrna superfamily v ... | 1993 | 8240957 |
| riemerella anatipestifer infection of domestic ducklings. | | 1997 | 9028598 |
| phylogenetic position of riemerella anatipestifer based on 16s rrna gene sequences. | riemerella anatipestifer, the causative agent of septicemia anserum exsudativa (also called new duckling disease), belongs to the family flavobacteriaceae of gram-negative bacteria. we determined the dna sequences of the rrs genes encoding the 16s rrnas of four r. anatipestifer strains by directly sequencing pcr-amplified rrs genes. a sequence similarity analysis confirmed the phylogenetic position of r. anatipestifer in the family flavobacteriaceae in rrna superfamily v and allowed fine mapping ... | 1997 | 9103649 |
| dna fingerprinting of riemerella anatipestifer. | seventeen restriction endonucleases were evaluated for use in dna fingerprinting of riemerella anatipestifer. digestion of chromosomal dna with either hinfi or ddei restriction endonuclease, followed by submarine electrophoresis in agarose and staining with ethidium bromide, resulted in dna fingerprint profiles that could be easily resolved. hinfi produced readable fingerprint patterns in the 2.7-20-kb range and was used to distinguish dna fingerprint profiles among 89 strains of r. anatipestife ... | 1998 | 9533086 |
| detection of acid production from carbohydrates by riemerella anatipestifer and related organisms using the buffered single substrate test. | one hundred and twenty-one riemerella anatipestifer field strains from wild birds, domesticated poultry and pigs were examined for their ability to produce acid from carbohydrates by using conventional biochemical and buffered single substrate (bss) test methods. the type strains of the species r. anatipestifer and taxometrically related genera chryseobacterium and bergeyella were included in the study. in contrast to 10 indole-positive r. anatipestifer variant strains, only a few of the 111 typ ... | 1998 | 9646458 |
| coenonia anatina gen. nov., sp. nov., a novel bacterium associated with respiratory disease in ducks and geese. | taxon 1502 was originally described as a riemerella anatipestifer-like bacterium causing exudative septicaemia in ducks and geese. in the present study, an integrated genotypic and phenotypic approach was used to elucidate the phylogenetic affiliation and taxonomic relationships of 12 strains of taxon 1502. whole-cell protein and fatty acid analyses and an extensive biochemical examination by using conventional tests and several api microtest systems indicated that all isolates formed a homogene ... | 1999 | 10319512 |
| molecular fingerprinting of riemerella anatipestifer by repetitive sequence pcr. | riemerella anatipestifer is a gram-negative rod-shaped bacterium associated with epizootic infections in poultry. a total of 35 r. anatipestifer isolates including the type strain atcc11845t, reference and field strains for 18 different serotypes were characterized by repetitive sequence based-pcr (rep-pcr) with outwardly-directed primers based on the repetitive extragenic palindromic (rep) consensus sequence. this technique was applied by using either extracted genomic dna or preparation of who ... | 1999 | 10418875 |
| identification of a virulence-associated protein homolog gene and isra1 in a plasmid of riemerella anatipestifer. | riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. we have previously reported that a 3.9-kb plasmid, pcfc1, carries protein genes (vapd1 and vapd2) that are similar to virulence-associated genes of other bacteria. in the present study, we report the complete sequence of a second plasmid of 5.6 kb, pcfc2. pcfc2 has a 28% g-c content and three large open reading frames (orfs). one of the orfs (designated asvapd1) encodes a polypeptide that shares 53.9, 53.9, 48.3 ... | 1999 | 10481080 |
| characterization of a predominant immunogenic outer membrane protein of riemerella anatipestifer. | the ompa gene, encoding the 42-kda major antigenic outer membrane protein ompa of riemerella anatipestifer, the etiololgical agent of septicemia anserum exsudativa, was cloned and expressed in escherichia coli. recombinant ompa displayed a molecular mass similar to that predicted from the nucleotide sequence of the ompa gene but lower than that observed in total cell lysates of r. anatipestifer. the ompa gene showed a conserved c-terminal region comprising the ompa-like domain and a variable n-t ... | 2000 | 10702488 |
| duck immune responses to riemerella anatipestifer vaccines. | riemerella anatipestifer (ra) infection is probably the most economically important infectious disease of farm ducks worldwide but the immune responses to natural infection and vaccines are poorly understood. we have used the lymphocyte transformation test (ltt) to study the expression of cell-mediated immunity (cmi), and the enzyme-linked immunosorbent assay to monitor antibody (ab) production following administration of formalin-inactivated and live attenuated serotype 2 (= g) ra vaccines. lym ... | 2000 | 10717285 |
| exclusion of strain 670/89 as type strain for serovar 20 of riemerella anatipestifer. | classical phenotypic characterisation and numeric analysis of whole-cell fatty acid patterns of twenty-one type strains of hitherto reported riemerella anatipestifer serovars revealed that the type strain of serovar 20 does not belong to the species riemerella anatipestifer sensu stricto and, therefore, it has to be excluded as a representative riemerella anatipestifer serotype strain. | 2000 | 10726364 |
| pathogenicity of a low-virulence duck virus enteritis isolate with apparent immunosuppressive ability. | duck enteritis virus (dev) was isolated from commercial 2-to-6-wk-old white pekin ducks experiencing 25%-30% mortality and high morbidity. secondary infections with pasteurella multocida, riemerella anatipestifer, and escherichia coli were frequently seen in affected ducks. the isolated virus was identical to the prototype dev by virus neutralization test but differed from the classic dev by causing lymphoid organ atrophy and inconsistent hemorrhagic lesions in the intestinal annular bands. atte ... | 2000 | 11007006 |
| polyamine distribution profiles in the eighteen genera phylogenetically located within the flavobacterium-flexibacter-cytophaga complex. | cellular polyamines of eighteen genera belonging to the flavobacterium-flexibacter-cytophaga complex were analysed by ion exchange liquid chromatography. homospermidine was the major polyamine in the genera bergeyella, riemerella, ornithobacterium, weeksella, capnocytophaga, polaribacter and psychroflexus belonging to the family flavobacteriaceae. in the family spirosomaceae, runella, spirosoma and flectobacillus species contained spermidine whereas cyclobacterium species contained homospermidin ... | 2001 | 11491527 |
| studies on the prevalence of riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains. | a total of 44 bacterial strains obtained from 49 clinically healthy ducklings of different ages originating from four different farms were identified as members of the species riemerella anatipestifer (ra) using conventional biochemical test methods. numerical analysis of the whole-cell fatty acid patterns of these isolates resulted in two different clusters, one of which showed a similar pattern to that of the type strain of ra. strains having a different fatty acid methyl esters (fame)-profile ... | 2001 | 11666036 |
| vaccination of ducks with recombinant outer membrane protein (ompa) and a 41 kda partial protein (p45n') of riemerella anatipestifer. | the generation of protective immunity against riemerella anatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (gst) fusion's proteins of ompa, a 42kda major outer membrane protein, and p45n', a 41kda n-terminal fragment of a newly identified 45kda potential surface protein from r. anatipestifer. the dna encoding ompa and p45n' were isolated from r. anatipestifer serotype 15 (field strain 110/89) and serotype 19 (reference strain 30/90) ... | 2002 | 11731174 |
| identification and characterization of camp cohemolysin as a potential virulence factor of riemerella anatipestifer. | riemerella anatipestifer is responsible for exudative septicemia in ducks. the genetic determinant of the camp cohemolysin, cam, from a strain of r. anatipestifer was cloned and expressed in escherichia coli. chromosomal dna from serotype 19 strain 30/90 was used to construct a gene library in pbluescript ii sk(-) vector in e. coli xl-1-blue strain. the clones containing recombinant plasmids were screened for the camp reaction with staphylococcus aureus. those that showed cohemolysis were chosen ... | 2002 | 11889100 |
| development of an elisa using a recombinant 41 kda partial protein (p45n') for the detection of riemerella anatipestifer infections in ducks. | riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. an indirect enzyme-linked immunosorbent assay (elisa) has been developed to facilitate early detection of r. anatipestifer infection in ducks. the antigen used was a recombinant 41 kda n-terminal fragment (rp45n') of a newly characterized r. anatipestifer potential surface protein, p45, which was expressed in escherichia coli as an n- ... | 2002 | 12220809 |
| a potential new serotype of riemerella anatipestifer isolated from ducks in thailand. | eighty isolates of riemerella anatipestifer representing 71 outbreaks of riemerellosis in thailand between 1994 and 1999 were serotyped using the gel diffusion precipitin test. based on the precipitation patterns, 25 serological profiles containing one to three antigenic determinants were recognized. heat-stable antigens of the organism reacted with antisera raised against 16 known serotypes and an untypable strain 698/95. the most prevalent serotype appeared to be serotype 7, followed by seroty ... | 2002 | 12396349 |
| antimicrobial susceptibility of riemerella anatipestifer isolated from ducks and the efficacy of ceftiofur treatment. | the in vitro susceptibilities of 50 field isolates of riemerella anatipestifer from ducks to ceftiofur and 16 other commonly used antimicrobials were determined. the mic90 values (mic refers to minimum inhibitory concentrations) for the antimicrobials used in this study are as follows: penicillin was 16 microg/ml; ceftiofur was 32 microg/ml; cephalothin, chloramphenicol, flumequine, and kanamycin were 64 microg/ml; nalidixic acid, nitrofurantoin, and sulfamethoxazole were 128 microg/ml; amikacin ... | 2003 | 12580291 |
| development and application of an elisa for the detection of duck antibodies against riemerella anatipestifer antigens in egg yolk of vaccinees and in serum of their offspring. | a direct and an indirect antibody enzyme-linked immunosorbent assay for duck yolk igy and duck serum igy was developed and tested on egg yolk and serum of ducks vaccinated with riemerella anatipestifer (ra). tests were performed either with primary antibodies labelled with horseradish peroxidase or with alkaline phosphatase-labelled secondary antibodies reacting with specifically bound rabbit anti-duck igy antibodies, respectively. ra-specific igys in egg yolk from three ducks increased rapidly ... | 2003 | 12675899 |
| cloning, expression, and purification of the virulence-associated protein d from xylella fastidiosa. | in this study, an efficient expression system, based on the pet32xa/lic vector, for producing a xylella fastidiosa virulence-associated protein d, found to have a strong similarity to riemerella anatipestifer and actinobacillus actinomycetencomitans vapd protein, is presented. the protein has a molecular mass of 17.637 da and a calculated pi of 5.49. the selected xfa0052 gene was cloned in the pet32xa/lic vector and the plasmid was transformed into escherichia coli bl21 (de3) strain at 37 degree ... | 2004 | 15358353 |
| kaistella koreensis gen. nov., sp. nov., a novel member of the chryseobacterium-bergeyella-riemerella branch. | gram-negative, non-spore-forming, rod-shaped, yellow-pigmented bacteria isolated from a freshwater stream in korea were investigated to determine their taxonomic position. complete 16s rrna gene sequence analysis indicated that the organisms should be placed in the chryseobacterium-bergeyella-riemerella branch in the family flavobacteriaceae. phylogenetically, the strains were most closely related to chryseobacterium balustinum atcc 33487(t) and chryseobacterium scophthalmum lmg 13028(t) (94.3 a ... | 2004 | 15545478 |
| sejongia antarctica gen. nov., sp. nov. and sejongia jeonii sp. nov., isolated from the antarctic. | two yellow-pigmented, gram-negative and aerobic bacterial strains, designated at1013t and at1047t, were isolated from terrestrial samples of the antarctic. on the basis of 16s rrna gene sequence analyses, the two antarctic strains shared 97.7 % sequence similarity and showed moderate relationships to the genera chryseobacterium (92.5-95.3 %), riemerella (92.3-93.5 %), bergeyella (92.5-92.6 %) and kaistella (92.5-93.3 %). in phylogenetic analyses, the two isolates formed a robust monophyletic cla ... | 2005 | 15653910 |
| genetic variation of the ompa and 16s rrna genes of riemerella anatipestifer. | the genetic diversity of the 16s rrna and ompa genes of riemerella anatipestifer was investigated. a 16s rrna gene-based pcr was able to amplify all 18 taiwanese strains and 10 reference strains. the identity of 16s rrna sequence of these strains and seven other sequences retrieved from genbank was 95.0-100.0%. the percentage identity of the ompa sequence of the 15 taiwanese strains and eight reference strains amplified in this study and two other sequences retrieved from genbank was 88.1-100.0% ... | 2005 | 15763739 |
| the effect of route of inoculation and challenge dosage on riemerella anatipestifer infection in pekin ducks (anas platyrhynchos). | riemerella anatipestifer is a gram-negative bacteria that can cause disease in a wide variety of wild and domesticated birds, especially waterfowl. the infection can be peracute, acute, or chronic. although various routes of transmission have been proposed, to date, there is little information on the effects of route of transmission and challenge dosage on r. anatipestifer infection. hence, the objective of this study was to determine the effect of route of inoculation and challenge dosage on r. ... | 2005 | 15839421 |
| transfer of chryseobacterium meningosepticum and chryseobacterium miricola to elizabethkingia gen. nov. as elizabethkingia meningoseptica comb. nov. and elizabethkingia miricola comb. nov. | the taxonomic positions of six strains (including the type strain) of chryseobacterium meningosepticum (king 1959) vandamme et al. 1994 and the type strain of chryseobacterium miricola li et al. 2004 were re-evaluated by using a polyphasic taxonomic approach. phylogenetic analysis, based on 16s rrna gene sequencing, showed that the strains represent a separate lineage from the type strains of the chryseobacterium-bergeyella-riemerella branch within the family flavobacteriaceae (90.7-93.9 % simil ... | 2005 | 15879269 |
| effects of anti-bursin monoclonal antibody on immunosuppression in the duck (cherry valley duck). | to study the immunologic function of bursin, we analyzed the effects of anti-bursin monoclonal antibody (mab) on the immunosuppression in ducks (cherry valley duck) by injecting various doses of the anti-bursin mab into 13-d duck embryos. after hatch, cell-mediated immune activity and humoral responses were studied using lymphocyte proliferation test, tube agglutination test, and indirect enzyme-linked immunosorbent assay to detect anti-escherichia coli antibodies and antibodies to riemerella an ... | 2006 | 16523625 |
| [rapid identification of riemerella anatipestifer on the basis of specific pcr amplifying 16s rdna]. | riemerella anatipestifer (ra) infection is the main disease causing severe losses in duck production. because ra is characterized more by the absence than by the presence of specific phenotypic properties and different scholar had the different results of biochemical detection, it can't always be identified quickly and correctly only by the phenotypic properties or biochemical characteristics. the research object was to develop a species specific pcr method for ra detection. because of the conse ... | 2006 | 16579457 |
| cloacibacterium normanense gen. nov., sp. nov., a novel bacterium in the family flavobacteriaceae isolated from municipal wastewater. | phenotypic and phylogenetic studies were performed on three isolates of an unknown gram-negative, facultatively anaerobic, non-motile, yellow-pigmented, rod-shaped organism isolated from raw sewage. 16s rrna gene sequence analysis indicated that these strains were members of the bergeyella-chryseobacterium-riemerella branch of the family flavobacteriaceae. the unknown bacterium was readily distinguished from reference strains by 16s rrna gene sequencing and biochemical tests. the organism contai ... | 2006 | 16738108 |
| dna fingerprinting of riemerella anatipestifer isolates from ducks. | | 2007 | 17209094 |
| development of a novel pcr assay specific for riemerella anatipestifer. | riemerella anatipestifer is a significant pathogen of waterfowl and turkeys. due to their similar ecology and morphological and cultural characteristics it is important to differentiate r. anatipestifer infections from those caused by pasteurella multocida. present study describes a novel pcr assay that is capable of rapid and species-specific identification of r. anatipestifer from bacterial cultures. | 2007 | 17257252 |
| genetic analysis of a duck circovirus detected in commercial pekin ducks in new york. | the genetic organization of the duck circovirus (ducv) 33753-52 detected in commercial pekin duck flocks from long island, ny, is described. the nucleotide sequence of virus 33753-52 exhibited high similarity with ducvs previously detected in germany and hungary. it is possible that this ducv from new york shares the same ancestor with the european counterparts. the virus 33753-52 exhibited genetic features characteristic of other circoviruses, such as the presence of two major open reading fram ... | 2007 | 17461272 |
| dose response study of enrofloxacin against riemerella anatipestifer septicaemia in muscovy and pekin ducklings. | the efficacy of enrofloxacin against riemerella anatipestifer (formerly pasteurella anatipestifer) septicaemia of muscovy and pekin ducklings was assessed in an artificial challenge model which reproduced typical duck riemerellosis with very high mortality. mortality, clinical signs, gross lesions, microbiological clearance, feed intake and body weight gain were the efficacy criteria studied. a pulsing medication in drinking water for 4 h at 25, 50 or 100 ppm enrofloxacin on the first day, follo ... | 1997 | 18483945 |
| phenotypic characteristics of riemerella anatipestifer and similar micro-organisms from various hosts. | a total of 199 riemerella anatipestifer (ra) and ra-like field strains isolated culturally from birds of 12 different species and from pigs were characterized using classical phenotypic and chemotaxonomic tests. the ra reference strain atcc 11845 was included in the study. on the basis of the classical phenotypic characteristics studied and the numerical analysis of the whole-cell fatty acid patterns, the ra reference strain and 123 field isolates were assigned to the indole negative (in) varian ... | 1998 | 18483963 |
| molecular characterization of a plasmid isolated from riemerella anatipestifer. | sixty strains of riemerella anatipestifer were isolated from ducks and geese with infectious serositis in taiwan. sixty per cent of the isolates (36/60) contained a 3.9 b plasmid, 12% (7/60) contained 6.5 b and 16 b plasmids, 5% (3/60) contained 2.9, 16 and 18 b plasmids and 13% contained no plasmid (14/60). the 3.9 b plasmid (designated as pcfc1) was completely sequenced to determine if it encoded virulence factors. pcfc1 was 27% g-c and had four large open reading frames (orf). two of the orfs ... | 1998 | 18484011 |
| genomic diversity and molecular differentiation of riemerella anatipestifer associated with eight outbreaks in five farms. | riemerella anatipestifer causes infectious serositis of ducks and geese. the genomic diversity of r. anatipestifer associated with outbreaks in waterfowls was studied using 24 multidrug-resistant r. anatipestifer isolates collected from the visceral organs of ducks and geese from seven outbreaks in four goose farms and one outbreak in one duck farm. seven methods were used to differentiate these isolates. plasmid patterns differed in plasmid number and size, ranging from 2.9 kb to 20 kb, and pro ... | 2008 | 18568653 |
| [analysis of outer membrane proteins of riemerella antipestifer]. | an isolated virulence riemerella anatipestifer strain passaged 200 times on tsb agar were used for the virulent to avirulent conversion. the effects of passage on biological properties of outer membrane proteins (omps) were investigated using the virulent and avirulent strains. transmission electron microscopy demonstrated that the avirulent strain produced lower amounts of outer membrane vesicles and the outer membrane decreased, the cytoplasmic appearance jumbled. the omps of the virulent stra ... | 2008 | 18616167 |
| new serotypes of riemerella anatipestifer isolated from ducks in thailand. | thirty-two riemerella anatipestifer isolates from ducks were serotyped by agar gel precepitin test using chicken antisera against serotypes 1 to 19 r. anatipestifer reference strains. the heat stable saline extracts from 29 field isolates reacted with the antisera of serotypes 1, 6, 7, 10, 11, 14, or 19. the isolates belonging to serotype 1 were the most prevalent (56.3%). antigens from the remaining three isolates did not react with any of the available antisera. additional investigations showe ... | 1995 | 18645776 |
| salpingitis in web-footed birds: prevalence, aetiology and significance. | among 4,893,447 ducks slaughtered during a 10 year period in a single slaughterhouse in denmark 7,190 (0.15%) were condemned because of salpingitis. a further 21,658 ducks (0.44%) were approved for human consumption after the inflamed salpinx had been removed. annual condemnation due to salpingitis varied from 0 to 0.53% and total condemnation varied from 1.30 to 2.72%, with an average of 1.98%. sixteen different bacterial species were isolated in pure culture from salpingitis in 7 week-old duck ... | 1995 | 18645801 |
| immunogenicity of riemerella anatipestifer broth culture bacterin and cell-free culture filtrate in ducks. | strains belonging to riemerella anatipestifer serotype 1 are responsible for most of the major disease outbreaks caused by this organism in ducks in thailand. therefore, the immunogenicity induced by a broth culture bacterin prepared from a local strain (1081) of this serotype was studied in ducks and a protective index (pi) was derived. the inactivated vaccine contained the equivalent of 5 x 10(9) colony-forming units of bacteria per 0.5-ml dose. a single subcutaneous inoculation of the broth c ... | 1996 | 18645893 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 18706944 |
| an investigation of duck circovirus and co-infection in cherry valley ducks in shandong province, china. | the co-infection of duck circovirus (ducv) with riemerella anatipestifer (ra) or/and escherichia coli (e. coli) or/and duck hepatitis virus i (dhv-i) in cherry valley ducks in china's shandong province was investigated by using polymerase-chain-reaction (pcr)-based methods. for this study, 742 ducks sampled at random from 70 duck farms during 2006-2007 were examined using pcr and dot-blot hybridisation (dbh) tests. overall the ducv infection rate was 33.29%. compared with those at 2 weeks of age ... | 2009 | 18760549 |
| development and evaluation of an antigen-capture elisa for detection of the ul24 antigen of the duck enteritis virus, based on a polyclonal antibody against the ul24 expression protein. | an antigen-capture enzyme-linked immunosorbent assay (ac-elisa) method was developed for the efficient detection of the ul24 antigen of the duck enteritis virus (dev) using polyclonal antibodies. ducks and rabbits were immunized, respectively, with expressed ul24 recombinant protein. the igg antibodies against ul24 from ducks and rabbits were purified and used as the capture antibodies. the specificity of the optimized ac-elisa was evaluated by use of dev, duck hepatitis virus (dhv), duck hepati ... | 2009 | 19467266 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 19475729 |
| planobacterium taklimakanense gen. nov., sp. nov., a member of the family flavobacteriaceae that exhibits swimming motility, isolated from desert soil. | a gram-negative and rod-shaped bacterial strain, x-65(t), isolated from desert soil of xinjiang, china, was subjected to phenotypic and phylogenetic studies. on the basis of 16s rrna gene sequence analyses, the isolate showed closest relationship to members of the genera chryseobacterium (92.4-95.4 % similarity), cloacibacterium (94.0 %), sejongia (93.9 %) and elizabethkingia (93.8 %) and to riemerella columbina lmg 11607(t) (93.3 %). the strain differed from its phylogenetic neighbours by its s ... | 2009 | 19542118 |
| phylogenetic relationships of riemerella anatipestifer serovars and related taxa and an evaluation of specific pcr tests reported for r. anatipestifer. | the aims of the present investigation were to characterize and identify serovars of riemerella anatipestifer and riemerella-like isolates genetically and to test the specificity of pcr tests reported for the identification of r. anatipestifer. | 2010 | 19840176 |
| identification of riemerella anatipestifer genes differentially expressed in infected duck livers by the selective capture of transcribed sequences technique. | riemerella anatipestifer is the causative agent of duck septicaemia. determination of r. anatipestifer virulence mechanisms will help us to effectively control this contagious agent. the differentially expressed gene profile of r. anatipestifer in infected duck livers was therefore identified and compared with in vitro cultures by selective capture of transcribed sequences analysis. a total of 48 genes were identified, of which 43 were genes that encode enzymes for amino acid biosynthesis and me ... | 2009 | 19937518 |
| pathogenesis of riemerella anatipestifer in turkeys after experimental mono-infection via respiratory routes or dual infection together with the avian metapneumovirus. | riemerella anatipestifer (ra) is the causative agent of septicaemic and exudative diseases in a variety of bird species. despite numerous outbreaks, little is known about the pathogenicity of ra for turkeys. we investigated the development of ra-induced disease in commercial turkey poults following ra inoculation via different respiratory routes. inoculation by aerosol or injection into the abdominal air sac led to systemic infection and mild gross lesions, including pericarditis, epicarditis an ... | 2009 | 19937539 |
| antibiotic susceptibility of riemerella anatipestifer field isolates. | kirby-bauer tests were used to analyze the antibiotic resistance of 224 isolates of riemerella anatipestifer isolated between 1998 and 2005. among the 36 antibiotics tested, 50% of the analyzed isolates were resistant to ampicillin, ceftazidime, aztreonam, cefazolin, cefepime, cefuroxime, oxacillin, penicillin g, rifampin, and trimethoprim/sulfamethoxazole. higher levels of resistance were detected for aztreonam, cefepime, oxacillin, penicillin g, ceftazidime, and trimethoprim/sulfamethoxazole ( ... | 2009 | 20095163 |
| antimicrobial peptides of an anti-lipopolysaccharide factor, epinecidin-1, and hepcidin reduce the lethality of riemerella anatipestifer sepsis in ducks. | antimicrobial peptides (amps) are effective against a wide range of microbes, but still no research results have reported their use in duck disease therapy. riemerella anatipestifer (ra) is a gram-negative bacterium which infects ducks and causes very significant economic losses. the minimum inhibitory concentrations (mics) of epinecidin-1 for the tested ra strains ranged 6.25-50microg/ml, those of the salf55-76 cyclic peptide ranged 12.5-25microg/ml, those of the salf55-76 linear peptide ranged ... | 2010 | 20138098 |
| characterization of biofilm formation by riemerella anatipestifer. | riemerella anatipestifer (ra) causes epizootics of infectious disease in poultry and results in serious economic losses, especially for the duck industry. the present study focuses on understanding the biofilm-producing ability of ra strains in attempt to explain the intriguing persistence of ra post-infection on duck farms. four ra serotype reference strains and 39 field ra isolates were measured for the biofilm formation by crystal violet staining. eighteen out of the 43 ra strains produced bi ... | 2010 | 20226600 |
| a thymidine kinase recombinant protein-based elisa for detecting antibodies to duck plague virus. | duck plague virus (dpv) is the causative agent of duck plague (dp) that causes significant morbidity and mortality throughout duck-producing areas of the world. the diagnosis of dp currently relies on the use of live or inactivated whole dpv virion as antigens in elisa, but it is too laborious and expensive for routine application, and it is still difficult to get purified dpv virion with current technology. | 2010 | 20416075 |
| epidemiologic investigation of riemerella anatipestifer in a commercial duck company by serotyping and dna fingerprinting. | a commercial duck company that raises approximately two million pekin ducks per year experienced an outbreak of riemerella anatipestifer (ra) on nine farms over a 1-yr period. owing to concerns that the bacteria was being spread from farm to farm, an investigation using serotyping and dna fingerprinting was performed. the results revealed that there were three different strains of ra involved in the outbreak. one strain was spread from one farm to six other farms, while another strain from the s ... | 2010 | 20608551 |
| isolation and characterization of atypical riemerella columbina strains from pigeons and their differentiation from riemerella anatipestifer. | riemerella columbina (rc) and riemerella anatipestifer (ra) belong to the genus riemerella within the family flavobacteriaceae. while ra is a well-described pathogen of waterfowl and other avian species, only little is known about rc. previous work reporting the isolation of rc from internal organs of clinically diseased pigeons suggested a potential pathogenic role in this avian species. in this study we examined pharyngeal swabs collected from pigeons and found rc to be widely distributed also ... | 2011 | 20615634 |
| expression of the c-terminal orf2 protein of duck astrovirus for application in a serological test. | duck astrovirus (dastv) is an important pathogen causing duck viral hepatitis (dvh), a highly contagious and fatal disease in young ducklings. to provide an antigen for a diagnostic serum test, the c-terminus of dastv orf2 protein was expressed in escherichia coli. four positive and 30 negative sera were used to validate the purified orf2 protein by developing an indirect enzyme-linked immunosorbent assay (elisa). no cross-reactions were found against other duck pathogens, including duck hepatit ... | 2010 | 20863856 |
| development and evaluation of an immunochromatographic strip test based on the recombinant ul51 protein for detecting antibody against duck enteritis virus. | duck enteritis virus (dev) infection causes substantial economic losses to the worldwide duck-producing areas. the monitoring of dev-specific antibodies is a key to evaluate the effect of dev vaccine and develop rational immunization programs. thus, in this study, an immunochromatographic strip (ics) test was developed for detecting dev serum antibodies. | 2010 | 20946624 |
| prevalence and molecular characterization of chloramphenicol resistance in riemerella anatipestifer isolated from ducks and geese in taiwan. | riemerella anatipestifer is a gram-negative bacterium that can cause disease in a wide range of wild and domesticated birds, especially waterfowl. the presence of an antibiotic-resistance gene in r. anatipestifer has not yet been reported, indicating the need for investigation. in the present study, 40.5% of r. anatipestifer isolates were found to exhibit resistance to chloramphenicol, while 45.9% showed intermediate resistance and 13.5% were susceptible to chloramphenicol, an antibiotic that ha ... | 2010 | 20954009 |
| loop-mediated isothermal amplification assay targeting the ompa gene for rapid detection of riemerella anatipestifer. | a novel loop-mediated isothermal amplification (lamp) assay was developed and evaluated for the detection of riemerella anatipestifer (ra) infection. the lamp assay exhibited a higher sensitivity than conventional polymerase chain reaction (pcr) and microbial isolation. the specificity of the assay was determined by restriction enzyme digestion of the lamp products and detection of escherichia coli, salmonella enterica and pasteurella multocida. the lamp assay was able to detect ra effectively i ... | 2010 | 21040782 |
| genome sequence of poultry pathogen riemerella anatipestifer strain ra-ym. | riemerella anatipestifer is a gram-negative, rod-shaped bacterium associated with epizootic infections in poultry. r. anatipestifer strain ra-ym, belonging to the serotype 1 prevalent in china, is a clinically isolated strain with high-level virulence. here, we report the first genome sequence of this species. | 2010 | 21183670 |
| development of a capture elisa to determine kinetics of soluble cd25 following in vitro and in vivo stimulation of duck peripheral blood monocytes. | in humans and other mammals, the α-chain of interleukin-2 (il-2) receptor (cd25) is induced and expressed on the cell surface after lymphocyte activation and is released from the membrane of activated cells as a smaller soluble form (scd25). however, little is known about avian scd25. in the present study, we developed an antigen capture enzyme-linked immunosorbent assay (ac-elisa) to detect serum scd25 in ducks, and we used flow cytometry (fcm) to analyze the frequency of cd25(+) cells in the p ... | 2010 | 21216015 |
| ompa is a virulence factor of riemerella anatipestifer. | riemerella anatipestifer infection is probably the most economically important disease of farm ducks worldwide. the pathogen r. anatipestifer causes septicemia anserum exsudativa in ducks, but little is known about the molecular basis of its pathogenesis and the virulence factors involved. in this study, by deleting ompa gene from r. anatipestifer serotype 2 strain th4, we constructed a mutant strain th4?ompa to investigate whether r. anatipestifer ompa is an important virulence factor. results ... | 2011 | 21349662 |
| complete genome sequence of the pathogenic bacterium riemerella anatipestifer strain ra-gd. | riemerella anatipestifer is a well-described pathogen of waterfowl and other avian species which can cause a great loss to the poultry industry. here we obtained the complete genome sequence of r. anatipestifer strain ra-gd, which was isolated from an infected duck in guangzhou, china, and was cultivated in our laboratory. | 2011 | 21441509 |
| development of multiplex pcr assay for rapid detection of riemerella anatipestifer, escherichia coli, and salmonella enterica simultaneously from ducks. | three pathogens, riemerella anatipestifer, escherichia coli, and salmonella enterica, are leading causes of bacterial fibrinous pericarditis and perihepatitis in ducks in china and worldwide. it is difficult to differentiate these pathogens when obtaining a diagnosis on clinical signs and pathological changes. the aim of this research was to develop a multiplex polymerase chain reaction (m-pcr) that could discriminate r. anatipestifer, e. coli, and s. enterica rapidly in field isolates, or detec ... | 2011 | 21791228 |
| detection of florfenicol resistance genes in riemerella anatipestifer isolated from ducks and geese. | the cat gene, coding for chloramphenicol acetyltransferase has been reported for conferring the chloramphenicol resistance for riemerella anatipestifer. chloramphenicol acetyltransferases, however, are unable to inactivate florfenicol. in this study, 66 r. anatipestifer isolates were investigated for their susceptibility to chloramphenicol and florfenicol and the presence of flor gene. results showed nine florfenicol intermediate or resistant r. anatipestifer isolates were all flor positive. the ... | 2011 | 21820820 |
| Complete genome sequence of Riemerella anatipestifer type strain (ATCC 11845). | Riemerella anatipestifer (Hendrickson and Hilbert 1932) Segers et al. 1993 is the type species of the genus Riemerella, which belongs to the family Flavobacteriaceae. The species is of interest because of the position of the genus in the phylogenetic tree and because of its role as a pathogen of commercially important avian species worldwide. This is the first completed genome sequence of a member of the genus Riemerella. The 2,155,121 bp long genome with its 2,001 protein-coding and 51 RNA gene ... | 2011 | 21677851 |
| the development of a rapid sybr green i-based quantitative pcr for detection of duck circovirus. | this report describes a one-step real-time polymerase chain reaction assay based on sybr green i for detection of a broad range of duck circovirus (ducv). align with all ducv complete genome sequences and other genus circovirus download from the genbank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of ducv were designed. the detection assay was linear in the range of 1.31 × 102-1.31 × 107 copies/μl. the reaction efficiency of the assay using the slope ( ... | 2011 | 21978576 |
| development of loop-mediated isothermal amplification (lamp) targeting the groel gene for rapid detection of riemerella anatipestifer. | riemerella anatipestifer (ra) infections cause major economic losses in the duck industry. detection of ra using conventional assays is time-consuming and laborious. in this study, a simple and rapid assay for the detection of ra was established based on the groel gene sequence of ra using loop-mediated isothermal amplification (lamp) with a set of six primers (two outer primers, two inner primers, and two loop primers). this assay was able to detect all the tested ra strains with different sero ... | 2011 | 22017034 |
| identification and immunological characteristics of chaperonin groel in riemerella anatipestifer. | riemerella anatipestifer (ra) infections cause major economic losses in the duck industry. in this study, an immunogenic protein, chaperonin groel (groel), was identified from the outer membrane of ra strain wj4 by immunoproteomic assay based on matrix-assisted laser desorption/ionization time of flight mass spectrometry. the complete sequence of the encoding gene, chaperonin groel (groel) was amplified and determined to be 1,629 base pairs in length. groel was then cloned into expression vector ... | 2011 | 22038245 |
| discovery and characterization of gene cassettes-containing integrons in clinical strains of riemerella anatipestifer. | forty-eight prevalent strains of riemerella anatipestifer (ra) isolated in china were tested for susceptibility to eighteen antibiotics and investigated for the frequencies and characteristics of integrons and gene cassettes. all isolates were resistant to between three and ten antimicrobial drugs. forty-seven isolates contained class 1 integron (97.92%), and 15 of the 47 isolates contained class 2 integron (31.25%). class 3 integron was not detected in the strains analysed. three different cass ... | 2011 | 22112855 |
| Ostrich - A new avian host of Riemerella columbina. | | 2012 | 22074857 |