| neisseria cuniculi in ruminants: epidemiological aspects. | neisseria cuniculi was isolated, between march 1987 and march 1989, from 38 cases of respiratory disease in small and large ruminants. in all but five cases n. cuniculi was cultured together with other potential respiratory pathogens. a survey was conducted to assess the prevalence of n. cuniculi in the pharyngeal region of merino and awassi purebred sheep, awassi/east-friesian and merino/romanov crossbred sheep and one exotic cross breed (goat/ibex). n. cuniculi was isolated from 80-88% of the ... | 1990 | 2249720 |
| isolation of neisseria cuniculi from a case of ovine pneumonia. | neisseria cuniculi was isolated in pure culture from the lungs of a 2-month-old pneumonic lamb. mouse ld50, evaluated by intraperitoneal and pulmonary inoculation of the microorganism, was 2 x 10(7) colony-forming units in both cases. the role of the organism in pathogenesis of the disease could not be determined. this organism has not previously been reported in sheep. | 1989 | 2609502 |
| relatedness of three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis, by dna-dna hybridizations and fatty acid analysis. | dna-dna hybridization was used to determine the levels of genomic relatedness of the three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis. the reference strains of these species exhibited high levels of intraspecies relatedness (93 to 100% for n. caviae, 79 to 100% for n. cuniculi, and 68 to 100% for n. ovis) but low levels of interspecific relatedness (less than 34%) to each other and to various species belonging to the beta subclass of the proteobacteri ... | 1993 | 8494736 |
| isolation and characterization of type iis restriction endonuclease from neisseria cuniculi atcc 14688. | neisseria cuniculi produces the restriction enzyme ncui which is an isoschizomer of mboii. we have demonstrated that ncui recognizes a pentanucleotide sequence (5'-gaaga-3'/3'-cttct-5'), and cleaves the dna 8 and 7 nucleotides downstream from the recognition site leaving a single 3'-protruding nucleotide. we have purified this enzyme to electrophoretic homogeneity using a four-step chromatographic procedure. ncui endonuclease is a monomeric protein with a m(r)=48,000+/-1000 under denaturing cond ... | 2001 | 11267775 |
| molecular characterization of the dna methyltransferase m1.ncui from neisseria cuniculi atcc 14688. | the methyltransferase m1.ncui is a member of the restriction-modification system in neisseria cuniculi atcc14688 and recognizes the asymmetric pentanucleotide sequence 5'-gaaga-3'/3'-cttct-5'. we purified m1.ncui to electrophoretic homogeneity using a four-step chromatographic procedure. m1.ncui is a protein with m(r)=32,000+/-1000 under denaturing conditions. it modifies the recognition sequence by transferring the methyl group from s-adenosyl-l-methionine to the 3' adenine of the pentanucleoti ... | 2007 | 17306509 |
| changes in abundance of lactobacillus spp. and streptococcus suis in the stomach, jejunum and ileum of piglets after weaning. | this present study investigated the changes in bacterial community composition, with an emphasis on lactobacillus spp. and streptococcus suis populations as potentially beneficial and harmful groups, in the stomach, jejunum and ileum of piglets after weaning (21 days postpartum) by 16s rrna gene-based methods. denaturing gradient gel electrophoresis analysis showed that, after weaning, predominant bands related to lactobacillus spp. disappeared and were replaced by potential pathogenic species, ... | 2008 | 18554303 |
| moraxella pluranimalium sp. nov., isolated from animal specimens. | four unusual gram-negative, catalase-positive, oxidase-positive, coccus-shaped bacteria isolated from one sheep and three pigs were characterized using phenotypic and molecular genetic methods. on the basis of cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus moraxella, although the organisms did not appear to correspond to any recognized species. comparative 16s rrna gene sequencing studies demonstrated that the isolates represent a novel subline ... | 2009 | 19329585 |
| m1.mboii and m2.mboii type iis methyltransferases: different specificities, the same target. | methylation of a base in a specific dna sequence protects the dna from nucleolytic cleavage by restriction enzymes recognizing the same sequence. the mboii restriction-modification (r-m) system of moraxella bovis atcc 10900 consists of a restriction endonuclease gene and two methyltransferase genes. the enzymes encoded by this system recognize an asymmetrical sequence 5'-gaaga-3'/3'-cttct-5'. m1.mboii modifies the last adenine in the recognition sequence 5'-gaaga-3' to n(6)-methyladenine. a seco ... | 2009 | 19332813 |
| moraxella porci sp. nov., isolated from pigs. | nine gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria were isolated from pigs affected by different pathological processes. phenotypic and genotypic methods were adopted to determine the relationships of these new isolates to recognized species of the genus moraxella. analysis of the 16s rrna gene sequences demonstrated that the clinical isolates represented a new lineage within the genus moraxella. the isolates were closely related to moraxella cuniculi and moraxella pluran ... | 2010 | 19946050 |