| isolation and characterization of rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. | several strains of phototrophic purple nonsulfur bacteria were isolated from colored blooms occurring in tidal and seawater pools in japan. all of these isolates had ovoid to rod-shaped cells that were motile by means of single polar flagella and contained vesicular intracytoplasmic membranes together with bacteriochlorophyll a and carotenoids of the spheroidene series. they produced ubiquinone 10 as the major quinone and contained straight-chain fatty acids, with c18:1 predominating. they were ... | 1995 | 7537066 |
| two-dimensional crystallization and preliminary structure analysis of light harvesting ii (b800-850) complex from the purple bacterium rhodovulum sulfidophilum. | light-harvesting complex ii (b800/850) from the purple bacterium rhodovulum (rhv.) sulfidophilum has been isolated using a new protocol. it has been shown by analytical ultracentrifugation and native gels to be most likely an octamer. two-dimensional crystals have been obtained by microdialysis. the plane group is p4212 with a = b = 157 a. the crystals diffract to 18 a in negative stain. projection maps show clearly that lhii is organized in ring-like particles with an outer diameter of about 76 ... | 1995 | 7602594 |
| complete assimilation of cysteine by a newly isolated non-sulfur purple bacterium resembling rhodovulum sulfidophilum (rhodobacter sulfidophilus). | a rod-shaped, motile, phototrophic bacterium, strain sicys, was enriched and isolated from a marine microbial mat, with cysteine as sole substrate. during phototrophic anaerobic growth with cysteine, sulfide was produced as an intermediate, which was subsequently oxidized to sulfate. the molar growth yield with cysteine was 103 g mol-1, in accordance with complete assimilation of electrons from the carbon and the sulfur moiety into cell material. growth yields with alanine and serine were propor ... | 1996 | 8661933 |
| two-dimensional structure of light harvesting complex ii (lhii) from the purple bacterium rhodovulum sulfidophilum and comparison with lhii from rhodopseudomonas acidophila. | within the membranes of photosynthetic bacteria, up to three types of light harvesting complexes (lhi, lhii, lhiii) are found. these complexes absorb photons and transfer the excitation energy to the photosynthetic reaction centre. the lh complexes comprise units that contain alpha and beta polypeptides with associated pigment molecules. | 1996 | 8805531 |
| gene cloning and regulation of gene expression of the puc operon from rhodovulum sulfidophilum. | rhodovulum (rhv.) sulfidophilum, unlike other nonsulfur purple bacteria, is able to synthesize the peripheral antenna complex even under fully aerobic conditions in the dark. we have obtained strong evidence that rhv. sulfidophilum encodes only one copy of the puc operon, comprising pucb, puca and pucc. pucb and puca encode the beta- and alpha-polypeptides. the third orf (pucc), downstream of puca, has a strong homology to pucc of rhodobacter (rb.) capsulatus. deletion mutation analysis indicate ... | 1997 | 9130598 |
| amaricoccus gen. nov., a gram-negative coccus occurring in regular packages or tetrads, isolated from activated sludge biomass, and descriptions of amaricoccus veronensis sp. nov., amaricoccus tamworthensis sp. nov., amaricoccus macauensis sp. nov., and amaricoccus kaplicensis sp. nov. | three isolates of gram-negative bacteria, strains ben 102t, ben 103t, and ben 104t, were obtained in pure culture by micromanipulation from activated sludge biomass from wastewater treatment plants in italy, australia, and macau, respectively. these isolates all had a distinctive morphology; the cells were cocci that usually were arranged in tetrads. based on this criterion, they resembled other bacteria from activated sludge previously called "g" bacteria. on the basis of phenotypic characteris ... | 1997 | 9226904 |
| biosynthesis of the 3-acetyl and 13(1)-oxo groups of bacteriochlorophyll a in the facultative aerobic bacterium, rhodovulum sulfidophilum--the presence of both oxygenase and hydratase pathways for isocyclic ring formation. | using (18)o-labelling and mass spectrometry, we have examined bacteriochlorophyll a formation in rhodovulum sulfidophilum, formerly known as rhodobacter sulfidophilus, which forms large amounts of bch1 a both aerobically in the dark and anaerobically in the light. r. sulfidophilum, growing under strict anaerobiosis in the light, possesses hydratases which incorporate (18)o label from h2(18)o into both the 13(1)-oxo and 3-acetyl oxygens; in addition, the four carboxyl oxygens at c13(3) and c17(3) ... | 1998 | 9799118 |
| heterologous expression of genes encoding bacterial light-harvesting complex ii in rhodobacter capsulatus and rhodovulum sulfidophilum. | in the present work we report the high-level expression of foreign genes encoding the light-harvesting (lhii) membrane-spanning polypeptides in photosynthetic bacteria. to do this we first constructed three deletion strains of rhodovulum (rhv.) sulfidophilum in which all or part of the puc operon, encoding the peripheral light-harvesting proteins, is missing. to investigate the heterologous expression of the light-harvesting polypeptides from rb. capsulatus in rhv. sulfidophilum and vice versa w ... | 1998 | 9880926 |
| repression of nitrogenase by ethanol in nitrogen-deprived cultures of rhodovulum sulfidophilum. | light-dependent h2 evolution did not occur in nitrogen-deprived cultures of rhodovulum sulfidophilum in the presence of ethanol. when ethanol was added to cells which had been grown with ammonia, derepression of the nitrogen fixation genes (nifhd) was inhibited at an ethanol concentration of 1 mm. on the other hand, when cells had nitrogenase-catalyzed proton-reducing activity prior to ethanol addition, reduction of the nifhd transcript level did not occur after the addition. in cells grown with ... | 1999 | 10077835 |
| the cytochrome bc1 complex from rhodovulum sulfidophilum is a dimer with six quinones per monomer and an additional 6-kda component. | a highly active, large-scale preparation of cytochrome bc1 complex has been obtained from the photosynthetic purple bacterium rhodovulum (rhv.) sulfidophilum. it has been characterized using mass spectrometry, quinone and lipid analysis as well as inhibitor binding. about 35 mg of pure complex can be obtained from 1 g of membrane protein. epr spectroscopy and optical titrations have been used to obtain the redox midpoint potentials of the cofactors. the em-value of 310 mv for the rieske protein ... | 1999 | 10092855 |
| a new cytochrome subunit bound to the photosynthetic reaction center in the purple bacterium, rhodovulum sulfidophilum. | the nucleotide sequence of the puf operon, which contains the genes encoding the b870 light-harvesting protein and the reaction center complex of the purple photosynthetic bacterium, rhodovulum sulfidophilum, was determined. the operon, which consisted of six genes, pufq, pufb, pufa, pufl, pufm, and pufc, is a new variety in photosynthetic bacteria in the sense that pufq and pufc coexist. the amino acid sequence of the cytochrome subunit of the reaction center deduced from the pufc sequence reve ... | 1999 | 10196154 |
| rhodovulum iodosum sp. nov. and rhodovulum robiginosum sp. nov., two new marine phototrophic ferrous-iron-oxidizing purple bacteria. | two new strains of marine purple bacteria, n1t and n2t, were isolated from coastal sediment of the north sea (germany) with ferrous iron as the only electron donor for anoxygenic photosynthesis. the isolates are the first salt-dependent, ferrous-iron-oxidizing purple bacteria characterized so far. analysis of 16s rrna gene sequences revealed an affiliation with the genus rhodovulum, which until now comprises only marine species. the sequence similarity of both strains was 95.2%, and their closes ... | 1999 | 10319496 |
| structural and functional analyses of photosynthetic regulatory genes rega and regb from rhodovulum sulfidophilum, roseobacter denitrificans, and rhodobacter capsulatus. | genes coding for putative rega, regb, and senc homologues were identified and characterized in the purple nonsulfur photosynthetic bacteria rhodovulum sulfidophilum and roseobacter denitrificans, species that demonstrate weak or no oxygen repression of photosystem synthesis. this additional sequence information was then used to perform a comparative analysis with previously sequenced rega, regb, and senc homologues obtained from rhodobacter capsulatus and rhodobacter sphaeroides. these are photo ... | 1999 | 10400577 |
| a spectroscopic method for observing the domain movement of the rieske iron-sulfur protein. | the g-tensor orientation of the chemically reduced rieske cluster in cytochrome bc(1) complex from rhodovulum sulfidophilum with respect to the membrane was determined in the presence and absence of inhibitors and in the presence of oxidized and reduced quinone in the quinol-oxidizing-site (q(o)-site) by epr on two-dimensionally ordered samples. almost identical orientations were observed when oxidized or reduced quinone, stigmatellin, or 5-(n-undecyl)-6-hydroxy-4,7-dioxobenzothiazole was presen ... | 2000 | 10681446 |
| transcriptional control of expression of genes for photosynthetic reaction center and light-harvesting proteins in the purple bacterium rhodovulum sulfidophilum. | the purple photosynthetic bacterium rhodovulum sulfidophilum synthesizes photosynthetic apparatus even under highly aerated conditions in the dark. to understand the oxygen-independent expression of photosynthetic genes, the expression of the puf operon coding for the light-harvesting 1 and reaction center proteins was analyzed. northern blot hybridization analysis showed that puf mrna synthesis was not significantly repressed by oxygen in this bacterium. high-resolution 5' mapping of the puf mr ... | 2000 | 10781546 |
| microaerobic hydrogen production by photosynthetic bacteria in a double-phase photobioreactor. | the rate of hydrogen production by the marine nonsulfur photosynthetic bacterium, rhodovulum sp., increased with increasing light intensity. a light intensity of 1800 w/m(2) hydrogen production rate was achieved at the rate of 9.4 micromol/mg dry weight/h. the hydrogen production of this strain was enhanced by the addition of a small amount of oxygen (12 micromol o(2)/reactor). intracellular atp content was most efficiently accumulated under microaerobic, dark conditions. hydrogen production rat ... | 2000 | 10799989 |
| [roseinatronobacter thiooxidans gen. nov., sp. nov., a new alkaliphilic aerobic bacteriochlorophyll-alpha-containing bacteria from a soda lake]. | several samples of microbial mat obtained from soda lakes of the kunkurskaya steppe (chita oblast) abundantly populated by purple bacteria were screened for the presence of heterotrophic alkaliphiles capable of oxidizing sulfur compounds to sulfate. this capacity was found in only one pigmented strain, alg 1, isolated on medium with acetate and thiosulfate at ph 10. the strain was found to be a strictly aerobic and obligately heterotrophic alkaliphile. growth on medium with acetate was possible ... | 2000 | 10808495 |
| deletion of the 6-kda subunit affects the activity and yield of the bc1 complex from rhodovulum sulfidophilum. | the cytochrome bc1 complex from rhodovulum sulfidophilum purifies as a four-subunit complex: the cytochrome b, cytochrome c1 and rieske iron-sulphur proteins, which are encoded together in the fbc operon, as well as a 6-kda protein. the gene encoding the 6-kda protein, named fbcs, has been identified. it is located within the sox operon, which encodes the subunits of sarcosine oxidase. the encoded 6-kda protein is very hydrophobic and is predicted to form a single transmembrane helix. it shows n ... | 2000 | 10848994 |
| rhodobaca bogoriensis gen. nov. and sp. nov., an alkaliphilic purple nonsulfur bacterium from african rift valley soda lakes. | from enrichment cultures established for purple nonsulfur bacteria using water and sediment samples from lake bogoria and crater lake, two soda lakes in the african rift valley, three strains of purple nonsulfur bacteria were isolated; strain lbb1 was studied in detail. cells of strain lbb1 were motile and spherical to rod-shaped, suggesting a relationship to rhodobacter or rhodovulum species, and the organism was capable of both phototrophic and chemotrophic growth on a wide variety of organic ... | 2000 | 10985738 |
| evidence for a functional similarity between the two-component regulatory systems regsr, actsr, and regba (prrba) in alpha-proteobacteria. | the symbiotic bacteria bradyrhizobium japonicum and sinorhizobium meliloti, and the purple photosynthetic bacteria rhodobacter capsulatus, rhodovulum sulfidophilum, roseobacter denitrificans and rhodobacter sphaeroides possess homologous two-component regulatory systems, namely regsr, actsr, regba and prrba. the respective response regulators of these bacteria control expression of different regulons that are involved in n2 fixation, co2 fixation, photosynthesis or acid tolerance. we therefore a ... | 2000 | 11131020 |
| an alternative to the accepted phylogeny of purple bacteria based on 16s rrna: analyses of the amino acid sequences of cytochromes c2 and c556 from rhodobacter (rhodovulum) sulfidophilus. | it is becoming increasingly apparent from complete genome sequences that 16s rrna data, as currently interpreted, does not provide an unambiguous picture of bacterial phylogeny. in contrast, we have found that analysis of insertions and deletions in the amino acid sequences of cytochrome c2 has some advantages in establishing relationships and that this approach may have broad utility in acquiring a better understanding of bacterial relationships. the amino acid sequences of cytochromes c2 and c ... | 2001 | 11361136 |
| phosphorylation of lhi beta during membrane synthesis in the photosynthetic bacterium rhodovulum sulfidophilum. | cells of rhv. sulfidophilum were grown under different conditions in the presence of 32p-phosphate and the corresponding h and l membrane fractions obtained and fractionated by sds-page. both membranes showed almost identical polypeptide composition. the bacteriochlorophyll (bchl) specific content in h was always lower that in l. as described before, oxygen did not regulate gene expression. under high light, an almost two- to threefold decrease of the cellular specific bchl content was observed. ... | 2001 | 11400052 |
| in vitro and in vivo electron transfer to the triheme cytochrome subunit bound to the photosynthetic reaction center complex in the purple bacterium rhodovulum sulfidophilum. | the cytochrome subunit bound to the photosynthetic reaction center (rc) complex in rhodovulum sulfidophilum lacks one heme-binding motif (cxxch) out of four motifs found in other purple bacteria resulting in the absence of the most distal heme from the rc-core complex (s. masuda et al., j. biol. chem. 274 (1999) 10795). cytochrome c(2), which acts as the electron donor to the rc was purified, and its gene was cloned and sequenced. the redox midpoint potential of cytochrome c(2) was determined to ... | 2001 | 11418094 |
| novel heme ligation in a c-type cytochrome involved in thiosulfate oxidation: epr and mcd of soxax from rhodovulum sulfidophilum. | the soxax complex of the bacterium rhodovulum sulfidophilum is a heterodimeric c-type cytochrome that plays an essential role in photosynthetic thiosulfate and sulfide oxidation. the three heme sites of soxax have been analyzed using electronic absorption, electron paramagnetic resonance, and magnetic circular dichroism spectroscopies. heme-3 in the ferric state is characterized by a large g(max) epr signal and has histidine and methionine axial heme iron ligands which are retained on reduction ... | 2001 | 11523998 |
| growth and production of biomass of rhodovulum sulfidophilum in sardine processing wastewater. | rhodovulum sulfidophilum was grown in sardine processing wastewater to assess growth characteristics for the production of bacterial biomass with simultaneous reduction of chemical oxygen demand. | 2001 | 11559398 |
| cytochrome complex essential for photosynthetic oxidation of both thiosulfate and sulfide in rhodovulum sulfidophilum. | many photosynthetic bacteria use inorganic sulfur compounds as electron donors for carbon dioxide fixation. a thiosulfate-induced cytochrome c has been purified from the photosynthetic alpha-proteobacterium rhodovulum sulfidophilum. this cytochrome c(551) is a heterodimer of a diheme 30-kda soxa subunit and a monoheme 15-kda soxx subunit. the cytochrome c(551) structural genes are part of an 11-gene sox locus. sequence analysis suggests that the ligands to the heme iron in soxx are a methionine ... | 2001 | 11567011 |
| evolutionary relationship of phototrophic bacteria in the alpha-proteobacteria based on farnesyl diphosphate synthase. | partial sequences of farnesyl diphosphate (fpp) synthase genes derived from the rhodobacter-rhodovulum group and from the rhodopseudomonas palustris-bradyrhizobium japonicum group of the alpha-proteobacteria were subjected to phylogenetic analysis to investigate the relationships of phototrophic and non-phototrophic bacteria in the alpha-proteobacteria . the four rhodovulum species formed a monophyletic group within the rhodobacter cluster, and agrobacterium ferrugineum iam 12616(t) intermingled ... | 2002 | 12055311 |
| molecular analysis of dimethyl sulphide dehydrogenase from rhodovulum sulfidophilum: its place in the dimethyl sulphoxide reductase family of microbial molybdopterin-containing enzymes. | dimethyl sulphide dehydrogenase catalyses the oxidation of dimethyl sulphide to dimethyl sulphoxide (dmso) during photoautotrophic growth of rhodovulum sulfidophilum. dimethyl sulphide dehydrogenase was shown to contain bis(molybdopterin guanine dinucleotide)mo, the form of the pterin molybdenum cofactor unique to enzymes of the dmso reductase family. sequence analysis of the ddh gene cluster showed that the ddha gene encodes a polypeptide with highest sequence similarity to the molybdopterin-co ... | 2002 | 12067345 |
| soxv, an orthologue of the ccda disulfide transporter, is involved in thiosulfate oxidation in rhodovulum sulfidophilum and reduces the periplasmic thioredoxin soxw. | proteins of the ccda/dsbd family have previously been found to be involved in the protein disulfide isomerase and cytochrome c maturation pathways of bacteria. soxv is a ccda homologue encoded by a genetic locus involved in lithotrophic thiosulfate oxidation in rhodovulum sulfidophilum. mutagenesis studies demonstrate an essential and specific role for soxv in thiosulfate oxidation. another protein encoded by the same locus, soxw, is a periplasmic thioredoxin. soxw was found to be in the reduced ... | 2002 | 12176044 |
| novel domain packing in the crystal structure of a thiosulphate-oxidizing enzyme. | a key component of the oxidative biogeochemical sulphur cycle involves the utilization by bacteria of reduced inorganic sulphur compounds as electron donors to photosynthetic or respiratory electron transport chains. the soxax protein of the photosynthetic bacterium rhodovulum sulfidophilum is a heterodimeric c-type cytochrome that is involved in the oxidation of thiosulphate and sulphide. the recently solved crystal structure of the soxax complex represents the first structurally characterized ... | 2002 | 12196153 |
| albidovulum inexpectatum gen. nov., sp. nov., a nonphotosynthetic and slightly thermophilic bacterium from a marine hot spring that is very closely related to members of the photosynthetic genus rhodovulum. | several bacterial isolates, with an optimum growth temperature of about 50 degrees c, were recovered from the marine hot spring at ferraria on the island of são miguel in the azores. the geothermal water emerged from a porous lava flow and rapidly cooled in contact with seawater except at low tide. the bacterial species represented by strains frr-10(t) and frr-11 was nonpigmented, strictly aerobic, and organotrophic. several genes, bchz, pufb, pufa, pufl, or pufm, encoding the photosynthetic rea ... | 2002 | 12200275 |
| mutational analyses of the photosynthetic reaction center-bound triheme cytochrome subunit and cytochrome c2 in the purple bacterium rhodovulum sulfidophilum. | the purple photosynthetic bacterium rhodovulum sulfidophilum has an unusual reaction center- (rc-) bound cytochrome subunit with only three hemes, although the subunits of other purple bacteria have four hemes. to understand the electron-transfer pathway through this subunit, three mutants of r. sulfidophilum were constructed and characterized: one lacking the rc-bound cytochrome subunit, another one lacking cytochrome c(2), and another one lacking both of these. the mutant lacking the rc-bound ... | 2002 | 12220186 |
| structural basis for the oxidation of thiosulfate by a sulfur cycle enzyme. | reduced inorganic sulfur compounds are utilized by many bacteria as electron donors to photosynthetic or respiratory electron transport chains. this metabolism is a key component of the biogeochemical sulfur cycle. the soxax protein is a heterodimeric c-type cytochrome involved in thiosulfate oxidation. the crystal structures of soxax from the photosynthetic bacterium rhodovulum sulfidophilum have been solved at 1.75 a resolution in the oxidized state and at 1.5 a resolution in the dithionite-re ... | 2002 | 12411478 |
| characterization of the redox centers in dimethyl sulfide dehydrogenase from rhodovulum sulfidophilum. | dimethyl sulfide dehydrogenase from the purple phototrophic bacterium rhodovulum sulfidophilum catalyzes the oxidation of dimethyl sulfide to dimethyl sulfoxide. recent dna sequence analysis of the ddh operon, encoding dimethyl sulfide dehydrogenase (ddhabc), and biochemical analysis (1) have revealed that it is a member of the dmso reductase family of molybdenum enzymes and is closely related to respiratory nitrate reductase (narghi). variable temperature x-band epr spectra (120-122 k) of purif ... | 2002 | 12484761 |
| maximization of hydrogen production ability in high-density suspension of rhodovulum sulfidophilum cells using intracellular poly(3-hydroxybutyrate) as sole substrate. | growth of and hydrogen production by wild-type (wt) rhodovulum sulfidophilum were compared with those by one of its mutants lacking the poly(3-hydroxybutyrate) (phb) biosynthesis ability (pnm2). during phototrophic growth under aerobic conditions with fixed illumination, changes in the extinction coefficient and phb content of wt and pnm2 cells revealed interference of light penetration by phb. wt cells synthesized phb at an early stage of the cultivation. phb degradation after exhaustion of ace ... | 2003 | 12491532 |
| farnesyl diphosphate synthase gene of three phototrophic bacteria and its use as a phylogenetic marker. | farnesyl diphosphate (fpp) synthase is essential not only for phototrophic bacteria in carotenoid biosynthesis, but also for non-phototrophic bacteria in the biosynthesis of physiologically important compounds. the gene encoding fpp synthase was assessed as a molecular marker to investigate the intermingled relationship between the phototropic and non-phototropic bacteria in the alpha-proteobacteria based on 16s rrna analysis. the fpp synthase amino acid sequences from three phototropic bacteria ... | 2002 | 12508853 |
| extracellular dna and rna produced by a marine photosynthetic bacterium rhodovulum sulfidophilum. | a marine photosynthetic bacterium rhodovulum sulfidophilum secretes nucleic acids that are involved in flocculating ability. these extracellular nucleic acids have not been well characterized. here, we have analyzed these nucleic acids and revealed that the extracellular nucleic acids are a mixture of double-stranded dnas and single-stranded rnas. the dnas have sizes of more than 30 kbp and at least a part of these dnas is probably an amplified portion of genomic dna. the rnas seems to be trna l ... | 2003 | 14510489 |
| cytochrome c551 from starkeya novella: characterization, spectroscopic properties, and phylogeny of a diheme protein of the soxax family. | cytochromes from the soxax family have a major role in thiosulfate oxidation via the thiosulfate-oxidizing multi-enzyme system (tomes). previously characterized soxax proteins from rhodovulum sulfidophilum and paracoccus pantotrophus contain three heme c groups, two of which are located on the soxa subunit. in contrast, the soxax protein purified from starkeya novella was found to contain only two heme groups. mass spectrometry showed that a disulfide bond replaced the second heme group found in ... | 2004 | 14645228 |
| rhodovulum sulfidophilum in the treatment and utilization of sardine processing wastewater. | rhodovulum sulfidophilum was grown in settled undiluted and nonsterilized sardine processing wastewater (spw). the aims were to evaluate the effects of inoculum size and media on the biomass production with simultaneous reduction of chemical oxygen demand (cod). | 2004 | 14687209 |
| structural and functional characterization of the unusual triheme cytochrome bound to the reaction center of rhodovulum sulfidophilum. | the cytochrome bound to the photosynthetic reaction center of rhodovulum sulfidophilum presents two unusual characteristics with respect to the well characterized tetraheme cytochromes. this cytochrome contains only three hemes because it lacks the peptide motif cxxch, which binds the most distal fourth heme. in addition, we show that the sixth axial ligand of the third heme is a cysteine (cys-148) instead of the usual methionine ligand. this ligand exchange results in a very low midpoint potent ... | 2004 | 15069076 |
| catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus sphingomonas for vigorous growth. | a bacterial strain, designated ast4(t), was isolated from activated sludge. the bacterium did not show significant growth on nutrient broth, but growth was clearly stimulated by addition of supernatant from other bacterial cultures. culture filtrate of a strain related to the genus sphingomonas in particular increased the cell yield and growth rate of strain ast4(t). phylogenetic analysis based on the 16s rrna gene sequences showed that strain ast4(t) is located within the 'rhodobacter group' in ... | 2004 | 15143049 |
| the nitrogen-fixing gene (nifh) of rhodopseudomonas palustris: a case of lateral gene transfer? | nitrogen fixation is catalysed by some photosynthetic bacteria. this paper presents a phylogenetic comparison of a nitrogen fixation gene (nifh) with the aim of elucidating the processes underlying the evolutionary history of rhodopseudomonas palustris. in the nifh phylogeny, strains of rps. palustris were placed in close association with rhodobacter spp. and other phototrophic purple non-sulfur bacteria belonging to the alpha-proteobacteria, separated from its close relatives bradyrhizobium jap ... | 2004 | 15256566 |
| thioclava pacifica gen. nov., sp. nov., a novel facultatively autotrophic, marine, sulfur-oxidizing bacterium from a near-shore sulfidic hydrothermal area. | strain tl 2(t) was isolated on mineral medium with thiosulfate from a near-shore sulfidic hydrothermal area in matupi harbour on the island of new britain, papua new guinea. the cells varied from long filaments with swollen ends, often aggregated, to short rods, depending on the growth conditions. the bacterium was obligately aerobic and grew autotrophically with thiosulfate as energy source or heterotrophically with organic acids and sugars. in thiosulfate-limited continuous culture, mu(max) an ... | 2005 | 15879235 |
| unusual accumulation of demethylspheroidene in anaerobic-phototrophic growth of crta-deleted mutants of rhodovulum sulfidophilum. | rhodovulum sulfidophilum produces carotenoids in the spheroidene pathway. spheroidene monooxygenase, crta, catalyzes the conversion of spheroidene to spheroidenone. crta-deleted mutants of r. sulfidophilum did not produce spheroidenone and demethylspheroidenone. in these mutants, the ratio of demethylspheroidene to spheroidene increased with exposure to light. one mutant exhibiting a spheroidene-predominant phenotype did not grow under anaerobic-light conditions and was devoid of bacteriochlorop ... | 2005 | 16086104 |
| [the phototrophic community found in lake khilganta (an alkaline saline lake located in the southeastern transbaikal region)]. | the structure of the phototrophic community found in lake khilganta (the agin-buryat autonomous area), a shallow saline soda lake (depth, 35-45 cm; water mineralization, 45 g/l; alkalinity, 30 mg-equiv/l; ph 9.5) has been studied. the bottom of the lake is covered with a 10- to 15-mm microbial mat, whose basis is formed by the filamentous cyanobacterium microcoleus chthonoplastes. the mat exhibits pronounced layering and contains a significant amount of minerals. six zones, which have characteri ... | 2005 | 16119856 |
| colorimetric dimethyl sulfide sensor using rhodovulum sulfidophilum cells based on intrinsic pigment conversion by crta. | a colorimetric whole-cell sensor for dimethyl sulfide (dms) was constructed based on the in vivo conversion of intrinsic pigments in response to the analyte. in a marine bacterium, rhodovulum sulfidophilum, carotenoids are synthesized via the spheroidene pathway. in this pathway, demethylspheroidene, a yellow carotenoid, is converted to spheroidene under catalysis of o-methyltransferase. spheroidene monooxygenase (crta) catalyzes the terminal step of the pathway and converts spheroidene to spher ... | 2006 | 16158287 |
| structural insight into the interactions of soxv, soxw and soxs in the process of transport of reductants during sulfur oxidation by the novel global sulfur oxidation reaction cycle. | microbial redox reactions involving inorganic sulfur compounds, mainly the sulfur anions, are one of the vital reactions responsible for the environmental sulfur balance. these reactions are mediated by phylogenetically diverse prokaryotes, some of which also take part in the extraction of metal ions from their sulfur containing ores. these sulfur oxidizers oxidize inorganic sulfur compounds like sulfide, thiosulfate etc. to produce reductants that are used for carbon dioxide fixation or in resp ... | 2006 | 16183190 |
| phylogenetic distribution of unusual triheme to tetraheme cytochrome subunit in the reaction center complex of purple photosynthetic bacteria. | to understand the evolutionary relationship between triheme and tetraheme cytochrome subunits in the reaction center complex, genes located downstream of that coding for the m subunit of the reaction center complex (pufm) were amplified by pcr and analyzed in six established and two unidentified species of the genus rhodovulum and five species of the genus rhodobacter. all the rhodovulum species tested had the pufc gene coding for the reaction-center-bound cytochrome subunit, while all the rhodo ... | 2004 | 16228402 |
| flocculating property of extracellular polymeric substance derived from a marine photosynthetic bacterium, rhodovulum sp. | the effect of the extracellular polymeric substance (eps) derived from a marine photosynthetic bacterium, rhodovulum sp., on the flocculation of kaolin is described. the amount of eps obtained and its flocculating activity were highly dependent on the extraction temperature and duration: high flocculating activity was obtained at a relatively low temperature (30 degrees c) and long extraction time (60 min). the eps was classified as a weak-anionic high-polymer group based on its ph profile, and ... | 1999 | 16232529 |
| biosorption of cadmium ions using a photosynthetic bacterium, rhodobacter sphaeroides s and a marine photosynthetic bacterium, rhodovulum sp. and their biosorption kinetics. | we examined the biosorption characteristics of cadmium ions onto a photosynthetic bacterium, rhodobacter sphaeroides s and a marine photosynthetic bacterium rhodovulum sp. ps88 in a batch culture system. both photosynthetic bacteria are capable of cadmium removal with 30 g/l sodium chloride and divalent cations (mg2+ and ca2+) in the culture medium. in particular, the strain ps88 shows a high removal ratio and high specific removal rate of cadmium ions from the culture medium under aerobic-dark ... | 2003 | 16233422 |
| roseibacterium elongatum gen. nov., sp. nov., an aerobic, bacteriochlorophyll-containing bacterium isolated from the west coast of australia. | a novel aerobic, chemoheterotrophic, bacteriochlorophyll-containing bacterium, strain och 323t, was isolated from sand at monkey mia, shark bay, located on the west coast of australia. the cells were gram-negative, non-motile rods of variable length; one or both cell poles was narrower. bacteriochlorophyll a was synthesized under aerobic conditions. catalase, oxidase and urease were produced. the onpg reaction was positive. the major component of the cellular fatty acid was octadecenoic acid (18 ... | 2006 | 16449450 |
| characterization of extracellular rnas produced by the marine photosynthetic bacterium rhodovulum sulfidophilum. | the marine photosynthetic bacterium rhodovulum sulfidophilum produces extracellular nucleic acids that are involved in its flocculation. these were found to be produced concomitantly with cell growth. the rna fraction of these extracellular nucleic acids was subjected to cdna analysis by applying a micro rna cloning method and found to contain mainly fully mature-sized trnas and fragments of 16s and 23s rrnas. analyses of modified bases and genes of the rnas revealed no structural difference bet ... | 2006 | 16672282 |
| whole-cell arsenite biosensor using photosynthetic bacterium rhodovulum sulfidophilum. rhodovulum sulfidophilum as an arsenite biosensor. | an arsenite biosensor plasmid was constructed in escherichia coli by inserting the operator/promoter region of the ars operon and the arsr gene from e. coli and the crta gene, which is responsible for carotenoid synthesis in the photosynthetic bacterium, rhodovulum sulfidophilum, into the broad-host-range plasmid vector, prk415. the biosensor plasmid, psense-as, was introduced into a crta-deleted mutant strain of r. sulfidophilum (cdm2), which is yellow in culture due to its content of spheroide ... | 2006 | 16733729 |
| characterization of a mutant strain of rhodovulum sulfidophilum lacking the pufa and pufb genes encoding the polypeptides for the light-harvesting complex 1 (b 870). | contradictory results on the effectiveness of energy transfer from the light harvesting complex 2 (lh2) directly to the reaction center (rc) in mutant strains lacking the core light-harvesting complex 1 (lh1) have been obtained with cells of rhodobacter capsulatus and rhodobacter sphaeroides. a lh1(-) mutant of rhodovulum sulfidophilum, named rslri, was constructed by deletion of the pufba genes, resulting in a kanamycin resistant photosynthetically positive clone. to restore the wild type pheno ... | 2006 | 16775747 |
| rhodovulum marinum sp. nov., a novel phototrophic purple non-sulfur alphaproteobacterium from marine tides of visakhapatnam, india. | a yellowish-brown bacterium was isolated from enrichment cultures inoculated with seawater samples from the eastern coast of india (visakhapatnam) under photoheterotrophic conditions. enrichment and isolation in a medium containing 2 % nacl (w/v) yielded strain ja128(t), which has ovoid to rod-shaped cells, also forms chains and is non-motile. phylogenetic analysis on the basis of 16s rrna gene sequences showed that strain ja128(t) clusters with the alphaproteobacteria and the sequence similarit ... | 2006 | 16825644 |
| astaxanthin formation in the marine photosynthetic bacterium rhodovulum sulfidophilum expressing crti, crty, crtw and crtz. | this study reports the production of astaxanthin in the photosynthetic bacterium rhodovulum sulfidophilum, which has adequate precursor pools and storage capabilities for heterologous carotenoid production. chemical mutagenesis was carried out using ethylmethane sulfonate to produce mutants with a modified carotenoid biosynthesis pathway downstream of phytoene. stable green- and gray-colored mutants were selected. green mutants contained neurosporene or chloroxanthin as their major carotenoid (> ... | 2006 | 17107420 |
| extracellular rnas produced by a marine photosynthetic bacterium rhodovulum sulfidophilum. | a marine photosynthetic bacterium rhodovulum sulfidophilum secretes nucleic acids. previously we have shown that these extracellular nucleic acids are a mixture of double-strand dnas and single-strand rnas. here, we have more closely analyzed the rna fraction of these extracellular nucleic acids. we determined the sequences of secreted rnas. they have same sequences with parts of rhodovulum sulfidophilum dsm 2351 16srrna or parts of ribosomal rna operon of related species. some of them were trna ... | 2004 | 17150530 |
| a new membrane-bound cytochrome c works as an electron donor to the photosynthetic reaction center complex in the purple bacterium, rhodovulum sulfidophilum. | a new type of membrane-bound cytochrome c was found in a marine purple photosynthetic bacterium, rhodovulum sulfidophilum. this cytochrome c was significantly accumulated in cells growing under anaerobic photosynthetic conditions and showed an apparent molecular mass of approximately 100 kda when purified and analyzed by sds-page. the midpoint potential of this cytochrome c was 369 mv. flash-induced kinetic measurements showed that this new cytochrome c can work as an electron donor to the photo ... | 2007 | 17197696 |
| rhodovulum imhoffii sp. nov. | a yellowish-brown bacterium was isolated from photoheterotrophic enrichment cultures obtained from water samples of an aquaculture pond at bhimunipatnam, india. enrichment and isolation in a medium containing 2 % nacl (w/v) yielded strain ja125t, the cells of which were rod-shaped and non-motile. on the basis of the 16s rrna gene sequence, strain ja125t belongs to the class alphaproteobacteria and is closely related to the type strains of rhodovulum iodosum (96 %), rhodovulum adriaticum (95 %), ... | 2007 | 17267955 |
| evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. | the change in carotenoid-based bacterial color from yellow to red can be applied to whole-cell biosensors. we generated several green mutants to emphasize the color change in such biosensors. the blue-green crti-deleted mutant, rhodopseudomonas palustris no.711, accumulated the colorless carotenoid precursor, phytoene. green rhodovulum sulfidophilum m31 accumulated neurosporene, a downstream product of phytoene. another green mutant, rhodobacter sphaeroides ga, accumulated neurosporene and chlor ... | 2007 | 17609942 |
| [seasonal changes in the structure of the anoxygenic photosynthetic bacterial community in lake shunet, khakassia]. | seasonal studies of the anoxygenic phototrophic bacterial community of the water column of the saline eutrophic meromictic lake shunet (khakassia) were performed in 2002 (june) and 2003 (february-march and august). from the redox zone down, the lake water was of dark green color. green sulfur bacteria predominated in every season. the maximum number of green sulfur bacteria was 10(7) cells/ml in summer and 10(6) cells/ml in winter. a multi-syringe stratification sampler was applied for the study ... | 2007 | 17633417 |
| rhodovulum visakhapatnamense sp. nov. | a gram-negative, rod-shaped, phototrophic bacterium (ja181(t)) was isolated from a tidal water sample. on the basis of 16s rrna gene sequence similarity, strain ja181(t) was shown to belong to the class alphaproteobacteria, most closely related to rhodovulum sulfidophilum (97.8 % similarity to the type strain), rhodovulum adriaticum (93 %), rhodovulum robiginosum (93 %), rhodovulum iodosum (94 %), rhodovulum imhoffii (94 %), rhodovulum strictum (95 %), rhodovulum euryhalinum (94.6 %) and rhodovu ... | 2007 | 17684252 |
| redox interaction of mn-bicarbonate complexes with reaction centres of purple bacteria. | it is found that dark reduction of photooxidized primary electron donor p870+ in reaction centres from purple anoxygenic bacteria (two non-sulphur fe-oxidizing rhodovulum iodosum and rhodovulum robiginosum, rhodobacter sphaeroides r-26 and sulphur alkaliphilic thiorhodospira sibirica) is accelerated upon the addition of mn2+ jointly with bicarbonate (30-75 mm). the effect is not observed if mn2+ and hco3(-) have been replaced by mg2+ and hco2(-), respectively. the dependence of the effect on bic ... | 2008 | 17971330 |
| [anoxygenic phototrophic bacterial community of lake shira (khakassia)]. | the anoxygenic phototrophic bacterial community of the brackish meromictic lake shira (khakassia) was investigated in august 2001, july 2002, and february-march 2003. in all the periods of investigation, the prevailing microorganisms were purple sulfur bacteria similar to lamprocystis purpurea in morphology and pigment composition. their highest number (3 x 10(5) cells/ml) was recorded in july 2002 at the depth of 15 m. according to 16s rrna gene analysis, the strain of purple sulfur bacteria is ... | 2007 | 17974211 |
| thermodynamic characterization of the redox centers within dimethylsulfide dehydrogenase. | dimethylsulfide (dms) dehydrogenase is a complex heterotrimeric enzyme that catalyzes the oxidation of dms to dmso and allows rhodovulum sulfidophilum to grow under photolithotrophic conditions with dms as the electron donor. the enzyme is a 164 kda heterotrimer composed of an alpha-subunit that binds a bis(molybdopterin guanine dinucleotide)mo cofactor, a polyferredoxin beta-subunit, and a gamma-subunit that contains a b-type heme. in this study, we describe the thermodynamic characterization o ... | 2008 | 18298089 |
| improvement of substrate conversion to molecular hydrogen by three-stage cultivation of a photosynthetic bacterium, rhodovulum sulfidophilum. | in photosynthetic bacteria, after transition to light-anaerobic and nitrogen-deficient conditions, hydrogen evolution starts with expression of nitrogenase activity. until the expression of enough activity, rhodovulum sulfidophilum consumed substrates and converted them to poly(3-hydroxybutyrate) (phb), resulting in a decrease in the proportion of substrate converted into hydrogen gas. to prevent conversion to phb during the period when nitrogenase activity is derepressed, the authors employed a ... | 1998 | 18575999 |
| rhodovulum kholense sp. nov. | a yellowish brown bacterium was isolated from photoheterotrophic enrichment cultures obtained from a mud sample collected from a mangrove forest located in khola, india. enrichment and isolation in medium containing 2 % nacl (w/v) yielded strain ja297(t), cells of which were ovoid and motile. cells of strain ja297(t) contained vesicular internal membranes and bacteriochlorophyll a and carotenoids of the spheroidene series. strain ja297(t) grew optimally at 30 degrees c and at ph 6.0-7.0. photo-o ... | 2008 | 18599724 |
| a mechanistic and electrochemical study of the interaction between dimethyl sulfide dehydrogenase and its electron transfer partner cytochrome c2. | dimethyl sulfide dehydrogenase isolated from the photosynthetic bacterium rhodovulum sulfidophilum is a heterotrimeric enzyme containing a molybdenum cofactor at its catalytic site, as well as five iron-sulfur clusters and a heme b cofactor. it oxidizes dimethyl sulfide (dms) to dimethyl sulfoxide in its native role and transfers electrons to the photochemical reaction center. there is genetic evidence that cytochrome c2 mediates this process, and the steady state kinetics experiments reported h ... | 2008 | 18607648 |
| heterologous synthesis and assembly of functional lhii antenna complexes from rhodovulum sulfidophilum in rhodobacter sphaeroides mutant. | the light harvesting complexes, including lhii and lhi, are the important components of photosynthetic apparatus. rhodovulum (rdv.) sulfidophilum and rhodobacter (r.) sphaeroides belong to two genera of photosynthetic bacteria, and they are very different in some physiological characteristics and light harvesting complexes structure. the lhii structural genes (pucbsas) from rdv. sulfidophilum and the lhi structural genes (pufba) from r. sphaeroides were amplified, and cloned into an expression v ... | 2009 | 18850303 |
| extracellular trnas of the marine photosynthetic bacterium rhodovulum sulfidophilum are not aminoacylated. | the marine photosynthetic bacterium rhodovulum sulfidophilum produces nucleic acids extracellularly. we have identified these extracellular rnas as fully mature sized trnas and fragments of 16s and 23s rrnas. most of the trnas have mature 3'-terminal cca sequences. in the present study we found that these extracellular trnas were not aminoacylated, although almost all intracellular trnas are aminoacylated. | 2009 | 19202284 |
| [nonsulfur purple bacteria of the southern transbaikal region and north-eastern mongolia soda lakes]. | | 2009 | 19449744 |
| characterization of extracellular dna production and flocculation of the marine photosynthetic bacterium rhodovulum sulfidophilum. | the marine photosynthetic bacterium rhodovulum sulfidophilum produces extracellular nucleic acids involved in its flocculation. previously, we showed that the rna fraction of these extracellular nucleic acids released into the culture medium contains mainly non-aminoacylated fully mature-sized trnas and fragments of 16s and 23s rrnas. here, we report the characterization of extracellular dna itself and its production during cultivation. no differences were detected in nucleotide sequence between ... | 2009 | 19452150 |
| rhodovulum lacipunicei sp. nov., an obligate sulfide-demanding phototrophic alphaproteobacterium isolated from a purple pond in india. | an ovoid-rod-shaped, phototrophic, purple non-sulfur bacterium, designated strain ja322(t), was isolated in pure culture from a water sample collected from a saline pond with purple-coloured water, located near satpada in orissa, india. strain ja322(t) was gram-negative and non-motile and grew photoheterotrophically with a number of organic compounds serving as carbon sources or electron donors. intracellular photosynthetic membranes were of the vesicular type. bacteriochlorophyll a and caroteno ... | 2009 | 19542142 |
| biochemistry and molecular biology of lithotrophic sulfur oxidation by taxonomically and ecologically diverse bacteria and archaea. | lithotrophic sulfur oxidation is an ancient metabolic process. ecologically and taxonomically diverged prokaryotes have differential abilities to utilize different reduced sulfur compounds as lithotrophic substrates. different phototrophic or chemotrophic species use different enzymes, pathways and mechanisms of electron transport and energy conservation for the oxidation of any given substrate. while the mechanisms of sulfur oxidation in obligately chemolithotrophic bacteria, predominantly belo ... | 2009 | 19645821 |
| rhodovulum steppense sp. nov., an obligately haloalkaliphilic purple nonsulfur bacterium widespread in saline soda lakes of central asia. | seven strains of purple nonsulfur bacteria isolated from the shallow-water steppe soda lakes of the cryoarid zone of central asia formed a genetically homogeneous group within the genus rhodovulum. the isolates were most closely related to rhodovulum strictum, from which they differed at the species level (99.5 % 16s rrna gene identity and 42-44 % dna-dna hybridization level). according to genotypic and phenotypic characteristics, the strains were assigned to a new species of the genus rhodovulu ... | 2010 | 19667383 |
| extracellular production of an rna aptamer by ribonuclease-free marine bacteria harboring engineered plasmids: a proposal for industrial rna drug production. | natural noncoding small rnas have been shown to be involved in a number of cellular processes as regulators. using the mechanisms thus elucidated, artificial small interfering rnas (sirnas), ribozymes, and rna aptamers are also expected to be potential candidates for rna therapeutic agents. however, current techniques are too costly for industrial production of these rnas for use as drugs. here, we propose a new method for in vivo production of artificial rnas using the marine phototrophic bacte ... | 2010 | 19966026 |
| the potential of cycloclasticus and altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments. | cycloclasticus sp. a5, which has been suggested to be a major degrader of petroleum aromatics spilled in temperate seas, showed higher degrading activities for petroleum aromatics, at both 25 degrees c and tropical sea temperature 30 degrees c, than the novel aromatic-degrading isolates, related to altererythrobacter epoxidivorans (97.5% similarity in the almost full-length 16s rrna gene sequence) and rhodovulum iodosum (96.3% similarity), obtained after enrichment on crude oil in a continuous s ... | 2010 | 20541115 |
| zeta potential of anoxygenic phototrophic bacteria and ca adsorption at the cell surface: possible implications for cell protection from caco(3) precipitation in alkaline solutions. | electrophoretic mobility measurements and surface adsorption of ca on living, inactivated, and heat-killed haloalkaliphilic rhodovulum steppense, a-20s, and halophilic rhodovulum sp., s-17-65 anoxygenic phototrophic bacteria (apb) cell surfaces were performed to determine the degree to which these bacteria metabolically control their surface potential equilibria. zeta potential of both species was measured as a function of ph and ionic strength, calcium and bicarbonate concentrations. for both l ... | 2011 | 21549386 |
| rhodovulum phaeolacus sp. nov. a phototrophic alphaproteobacterium isolated from a brown pond. | two strains of oval-rod shaped, gram-negative, phototrophic, purple non-sulfur bacteria designated ja580(t) and ja595 were isolated from a sediment sample collected from a brown pond. strain ja580(t) was designated as the type strain, while strain ja595 as an additional strain has similar characteristics to the type strain. strain ja580(t) was non-motile and grew photoheterotrophically with a number of organic compounds serving as carbon source/electron donor. intracellular photosynthetic membra ... | 2011 | 21817826 |
| [In Process Citation]. | | 2011 | 22168007 |
| Chemical and structural status of copper associated with oxygenic and anoxygenic phototrophs and heterotrophs: possible evolutionary consequences. | Copper adsorption on the surface and intracellular uptake inside the cells of four representative taxons of soil and aquatic micro-organisms: aerobic rhizospheric heterotrophs (Pseudomonas aureofaciens), anoxygenic (Rhodovulum steppense) and oxygenic (cyanobacteria Gloeocapsa sp. and freshwater diatoms Navicula minima) phototrophs were studied in a wide range of pH, copper concentration, and time of exposure. Chemical status of adsorbed and assimilated Cu was investigated using in situ X-ray abs ... | 2011 | 22039921 |
| Defluviimonas denitrificans gen. nov., sp. nov., and Pararhodobacter aggregans gen. nov., sp. nov., non-phototrophic Rhodobacteraceae from the biofilter of a marine aquaculture. | Three Gram-negative bacterial strains were isolated from the biofilter of a recirculating marine aquaculture. They were non-pigmented rods, mesophiles, moderately halophilic, and showed chemo-organoheterotrophic growth on various sugars, fatty acids, and amino acids, with oxygen as electron acceptor; strains D9-3(T) and D11-58 were in addition able to denitrify. Phototrophic or fermentative growth could not be demonstrated. Phylogenetic analysis of the 16S rRNA gene sequences placed D9-3(T) and ... | 2011 | 21959289 |
| artificial rna aptamer production by the marine bacterium rhodovulum sulfidophilum: improvement of the aptamer yield using a mutated transcriptional promoter. | noncoding small rnas and artificial rna aptamers are now expected to be potential candidates for rna therapeutic agents. we previously proposed a unique method for economical production of these rnas using the marine phototrophic bacterium rhodovulum sulfidophilum. this bacterium does not produce any ribonucleases but does produce extracellular nucleic acids in the culture medium in nature. using this bacterium and an engineered plasmid containing the rrn promoter for the rna expression, we deve ... | 2011 | 21903467 |
| Rhodovulum bhavnagarense sp. nov., a phototrophic alphaproteobacterium isolated from a pink pond. | An oval to rod shaped, Gram-stain-negative, phototrophic bacterium designated as strain JA738T was isolated from a sediment sample collected from a pink pond. Strain JA738T was non-motile and has vesicular type intracellular photosynthetic membranes. Bacteriochlorophyll a and carotenoids of the spheroidene series were present as the major photosynthetic pigments. Strain JA738T requires thiamine and pantothenate for growth. C18:1?7c, C18:1?5c, C18:0, C18:1?7c11-methyl are the major cellular fatty ... | 2011 | 22180610 |