| isolation and continuous culture of neorickettsia helminthoeca in a macrophage cell line. | experimental evidence is presented supporting the development of a system for the isolation and propagation of a neorickettsia sp. in a continuous canine macrophage cell line (dh82). to isolate a neorickettsia sp. pathogenic to the canine species, three naive dogs were fed metacercaria-encysted kidneys of salmon caught in a river where infection of metacercariae with neorickettsia helminthoeca has been circumstantially known for decades. clinically, the classic course of salmon poisoning disease ... | 1991 | 1774317 |
| cross-reacting antigens between neorickettsia helminthoeca and ehrlichia species, shown by immunofluorescence and western immunoblotting. | dogs orally infected with neorickettsia helminthoeca developed immunoglobulin g titers against erlichia risticii, erlichia sennetsu, and erlichia canis similar to those against n. helminthoeca antigen, as determined by immunofluorescence. western immunoblotting showed that the major common antigens shared among the microorganisms were 80- or 78-kda and 64-kda polypeptides. in contrast, horse anti-e. risticii and anti-e. sennetsu and dog anti-e. canis sera reacted more weakly to n. helminthoeca a ... | 1991 | 1774330 |
| salmon poisoning disease in juvenile coyotes: clinical evaluation and infectivity of metacercariae and rickettsiae. | clinical salmon poisoning disease (spd), and survival of neorickettsia helminthoeca and metacercariae of nanophyetus salmincola in fish were evaluated experimentally in 12-wk-old coyotes (canis latrans) to determine the potential of spd for biological control of coyotes. coyotes readily ate fish that contained metacercariae and rickettsiae. they developed diarrhea, anorexia and lethargy within 7 days after feeding. infected coyotes lost 58% of their body weight when compared to uninfected contro ... | 1987 | 3625898 |
| an improved staining method for neorickettsia helminthoeca. | | 1965 | 4160493 |
| in vitro cultivation of neorickettsia helminthoeca, the causative agent of salmon poisoning disease. | | 1972 | 4558492 |
| preparation of complement-fixing antigens from tissue of dogs infected with neorickettsia helminthoeca. | | 1973 | 4693812 |
| a new experimental second intermediate host of nanophyetus salmincola with evidence of transmission of neorickettsia helminthoeca. | | 1970 | 5421098 |
| ehrlichia-like rickettsiosis in dogs in oklahoma and its relationship to neorickettsia helminthoeca. | | 1966 | 5913043 |
| neorickettsia-like organism isolated from metacercaria of a fluke, stellantchasmus falcatus. | "hyuga" fever, reportedly caused by rickettsia sennetsu, occurring in the west of japan was suspected to be an endoparasite-borne rickettsiosis. no r. sennetsu, however, was detected in metacercariae found in the muscles of grey mullets (mugil cephalus) ingested raw by most "hyuga" fever patients. instead, another neorickettsia-like organism (sf strain) was isolated occasionally from metacercariae of stellantchasmus falcatus. a dog was inoculated with sf strain. it developed mild fever after an ... | 1981 | 7311106 |
| 16s rrna gene sequence of neorickettsia helminthoeca and its phylogenetic alignment with members of the genus ehrlichia. | neorickettsia helminthoeca (tribe ehrlichieae, family rickettsiaceae) is the agent of salmon poisoning disease, which affects members of the family canidae. this bacterium is unusual in that it is the only known obligately intracellular bacterium that is transmitted via a helminth vector. the nucleotide sequence of the n. helminthoeca 16s rrna gene was determined and compared with the sequences of intracellular bacteria belonging to the alpha subgroup of the proteobacteria. the n. helminthoeca s ... | 1995 | 7537055 |
| cytopathic effect, plaque formation, and lysis of ehrlichia chaffeensis grown on continuous cell lines. | ehrlichiae are strict intracellular bacterial pathogens that parasitize leukocytes or other blood cells. only six agents of the tribe ehrlichieae, namely, cowdria ruminantium, neorickettsia helminthoeca, ehrlichia risticii, ehrlichia sennetsu, ehrlichia canis, and ehrlichia chaffeensis, have been adapted to growth in continuous cell lines. e. chaffeensis, the agent of human ehrlichiosis, has been cultured only in a cell line of canine origin. we adapted purified cell-free e. chaffeensis for grow ... | 1994 | 8300201 |
| characterization of ehrlichial organisms isolated from a wild mouse. | an infectious agent was isolated from the enlarged spleen of a wild mouse, eothenomys kageus, by intraperitoneal inoculation of the spleen homogenate into laboratory mice. the laboratory mice developed splenomegaly, and the agent was maintained by serial passage of spleen homogenates in laboratory mice. the agent in the spleen homogenate was inactivated after incubation at 37 or 50 degrees c. tetracyclines were effective in preventing infection of mice with this agent, but penicillin and sulfona ... | 1993 | 8380184 |
| characterization of the sf agent, an ehrlichia sp. isolated from the fluke stellantchasmus falcatus, by 16s rrna base sequence, serological, and morphological analyses. | the organism designated the sf agent was originally isolated in japan in 1962 from stellantchasmus falcatus metacercaria parasitic on gray mullet fish. the sf agent resembles members of the genus ehrlichia morphologically and exhibits weak antigenic cross-reactivity with ehrlichia sennetsu. this organism causes mild clinical signs in dogs, but severe splenomegaly and lymphadenopathy in mice. this suggests that the sf agent may be similar to either neorickettsia helminthoeca, an intracellular par ... | 1996 | 8573488 |
| comparison of nested pcr with immunofluorescent-antibody assay for detection of ehrlichia canis infection in dogs treated with doxycycline. | a partial 16s rrna gene was amplified in ehrlichia canis-infected cells by nested pcr. the assay was specific and did not amplify the closely related ehrlichia chaffeensis, ehrlichia muris, neorickettsia helminthoeca, and sf agent 16s rrna genes. the assay was as sensitive as southern hybridization, detecting as little as 0.2 pg of e. canis dna. by this method, all blood samples from four dogs experimentally infected with e. canis were positive as early as day 4 postinoculation, which was before ... | 1997 | 9196207 |
| molecular detection of an ehrlichia-like agent in rainbow trout (oncorhynchus mykiss) from northern california. | ehrlichia dna was identified by nested pcr in rainbow trout (oncorhynchus mykiss) collected from a creek in northern california where potomac horse fever is endemic. ehrlichia dna was found in tissues from several organs including the gills, heart, spleen, liver, kidneys and intestine of trout and from three different adult digenetic trematodes (deropegus sp., crepidostomum sp., creptotrema sp.) parasitizing the gallbladder and/or the intestine of the trout. sequencing of pcr-amplified dna from ... | 2000 | 10962157 |
| citrate synthase gene sequence: a new tool for phylogenetic analysis and identification of ehrlichia. | the sequence of the citrate synthase gene (glta) of 13 ehrlichial species (ehrlichia chaffeensis, ehrlichia canis, ehrlichia muris, an ehrlichia species recently detected from ixodes ovatus, cowdria ruminantium, ehrlichia phagocytophila, ehrlichia equi, the human granulocytic ehrlichiosis [hge] agent, anaplasma marginale, anaplasma centrale, ehrlichia sennetsu, ehrlichia risticii, and neorickettsia helminthoeca) have been determined by degenerate pcr and the genome walker method. the ehrlichial ... | 2001 | 11526124 |
| analysis of p51, groesl, and the major antigen p51 in various species of neorickettsia, an obligatory intracellular bacterium that infects trematodes and mammals. | the p51 gene that encodes the major antigenic 51-kda protein in neorickettsia risticii was identified in strains of neorickettsia sennetsu and the stellantchasmus falcatus agent but not in neorickettsia helminthoeca, suggesting that p51-based diagnosis would be useful to distinguish among them. groesl sequencing results delineated the phylogenic relationships among neorickettsia spp. | 2004 | 15297539 |
| suspected cases of neorickettsia-like organisms in brazilian dogs. | preliminary findings of gross and histopathological lesions consistent with salmon poisoning disease in 10 dogs from southern brazil are described. lesions were restricted to the spleen, lymph nodes, and intestinal lymphoid tissues. grossly, there was marked hyperplasia of mesenteric lymph nodes and peyer's patches. microscopic alterations were characterized by diffuse hyperplasia of intestinal lymphoid tissues and peyer's patches. intracytoplasmic organisms consistent with neorickettsia helmint ... | 2004 | 15604473 |
| lymph node aspirate from a california wine-country dog. | a 4-year-old, male golden retriever was presented to the veterinary medical teaching hospital at the university of california-davis with a history of lethargy, inappetance, and vomiting. the patient had generalized lymphadenomegaly, marked thrombocytopenia, mild anemia, and moderate hypoalbuminemia. moderate to marked histiocytic inflammation and lymphocytic-plasmacytic reactivity of the mesenteric, left popliteal, and right mandibular lymph nodes were diagnosed cytologically. many macrophages c ... | 2006 | 16783722 |
| neorickettsia helminthoeca in dog, brazil. | | 2006 | 16972361 |
| salmon poisoning disease in dogs on southern vancouver island. | salmon poisoning disease in dogs has previously been reported in north america only along the western coast of the u.s.a. this paper presents the findings from eight affected dogs recently diagnosed on vancouver island, canada. the clinical signs shown by these dogs were lethargy, anorexia, pyrexia and lymph node enlargement. the causative agent, neorickettsia helminthoeca was observed in macrophages obtained from lymph node aspirates. this organism is transmitted to dogs in cysts of the fluke n ... | 1984 | 17422349 |
| salmon poisoning disease in two malayan sun bears. | 2 captive sun bears (helarctos malayanus) were evaluated because of acute onset of vomiting, mucoid diarrhea, lethargy, and anorexia 1 week after eating live trout from a northern california reservoir. | 2008 | 18279100 |
| neorickettsia helminthoeca in brazilian dogs: a cytopathological, histopathological and immunohistochemical study. | | 2009 | 19374648 |
| neorickettsia helminthoeca and salmon poisoning disease: a review. | neorickettsia helminthoeca is an obligate intra-cytoplasmic bacterium that causes salmon poisoning disease (spd), an acute, febrile, fatal disease of dogs. the complex life-cycle of this pathogen involves stages in an intestinal fluke (nanophyetus salmincola), a river snail (oxytrema silicula), in fish, and in fish-eating mammals. this complexity has created confusion with respect to the various bacterial and parasitic infections associated with the disease and its significance in dogs in specif ... | 2011 | 20044285 |
| salmon poisoning disease in dogs: 29 cases. | salmon poisoning disease (spd) is a trematode-borne disease of dogs caused by neorickettsia helminthoeca. | 2010 | 20337917 |
| nanophyetus salmincola, vector of the salmon poisoning disease agent neorickettsia helminthoeca, harbors a second pathogenic neorickettsia species. | the trematode nanophyetus salmincola is known as the carrier of neorickettsia helminthoeca, an obligate intracellular endosymbiotic bacterium that causes salmon poisoning disease (spd), a fatal disease of dogs. the bacteria are maintained through the complex life cycle of n. salmincola that involves snails juga plicifera as the first intermediate host, salmonid fishes as the second intermediate host and fish-eating mammals as definitive hosts. n. salmincola was also found to harbor a second spec ... | 2016 | 27809964 |
| analysis of complete genome sequence and major surface antigens of neorickettsia helminthoeca, causative agent of salmon poisoning disease. | neorickettsia helminthoeca, a type species of the genus neorickettsia, is an endosymbiont of digenetic trematodes of veterinary importance. upon ingestion of salmonid fish parasitized with infected trematodes, canids develop salmon poisoning disease (spd), an acute febrile illness that is particularly severe and often fatal in dogs without adequate treatment. we determined and analysed the complete genome sequence of n. helminthoeca: a single small circular chromosome of 884 232 bp encoding 774 ... | 2017 | 28585301 |