| phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera caryophanon, exiguobacterium, kurthia, and planococcus. | the 16s rrna gene sequences of "bacillus aminovorans" and several species considered to be phylogenetically related to the group 2 bacilli of ash et al. (c. ash, j. a. e. farrow, s. wallbanks, and m. d. collins, lett. appl. microbiol. 13:202-206, 1991) were determined. a comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa (the genera caryophanon, exiguobacterium, kurthia, planococcus), form a phylogenetically disti ... | 1994 | 8123563 |
| exiguobacterium undae sp. nov. and exiguobacterium antarcticum sp. nov. | four orange-pigmented strains from pond water (l1-l4) have been subjected to polyphasic taxonomic analyses. on the basis of ribotype analysis and fourier-transform infrared spectroscopy, these strains form a genomically highly related group. 16s rdna sequence analysis revealed 98.8% similarity between the 16s rdna sequences of strains l2t and h2t, isolated previously from a microbial mat from lake fryxell, antarctica. dna-dna reassociation values indicated the presence of two genomic clusters. w ... | 2002 | 12148624 |
| novel components of leaf bacterial communities of field-grown tomato plants and their potential for plant growth promotion and biocontrol of tomato diseases. | this work aimed to characterize potentially endophytic culturable bacteria from leaves of cultivated tomato and analyze their potential for growth promotion and biocontrol of diseases caused by botrytis cinerea and pseudomonas syringae. bacteria were obtained from inner tissues of surface-disinfected tomato leaves of field-grown plants. analysis of 16s rrna gene sequences identified bacterial isolates related to exiguobacterium aurantiacum (isolates bt3 and mt8), exiguobacterium spp. (isolate gt ... | 2016 | 26654914 |
| possible involvement of a single histidine residue in the p-type na+-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | effect of various inhibitors on the p-type na+-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, was examined. the atpase was extremely sensitive to p-chloromercuriphenylsulfonic acid, a modifier of sh-group. the enzyme was also sensitive to diethylpyrocarbonate, and analysis of the inhibition kinetics by the drug indicated that modification of a single histidine residue per atpase molecule was sufficient to inactivate the enzyme. | 2001 | 11685505 |
| characterization of a p-type na+-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, possesses a p-type na(+)-stimulated atpase in the membrane (koyama, n. (1999) curr. microbiol. 39, 27-30). in this study, we attempted to purify and characterize the enzyme. the atpase appears to consist of a single polypeptide with an apparent molecular mass of 100 kda. the enzyme exhibited an optimum ph for activity at around 9. the enzyme was strongly inhibited by vanadate (50% inhibition observed at 3 microm) and forms an ac ... | 2000 | 10799538 |
| purification and properties of a novel azide-sensitive atpase of exiguobacterium aurantiacum. | exiguobacterium aurantiacum bl77/1 possesses at least two distinct membrane-bound atpases. one of them was solubilized with decanoyl n-methylglucamide, a non-ionic detergent, and purified by successive chromatography on deae-sepharose and hydroxyapatite. the purified atpase appears to consist of a single polypeptide component with an apparent molecular mass of 54 kda. among the triphosphates of various nucleosides tested, atp was the best substrate. the enzyme exhibited a km of 0.5 mm for atp an ... | 2000 | 10763752 |
| presence of na+-stimulated p-type atpase in the membrane of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | it was found that a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, possesses a membrane-bound atpase, which was activated specifically by na+. the na+-stimulated atpase activity reached a maximum value at 200 mm nacl. in the presence of 200 mm nacl, the activity was drastically reduced by vanadate, a potent inhibitor of p-type atpase, with a half-maximal inhibition at 1 microm. incubation of the membranes with [gamma-32p]atp followed by acidic lithium dodecyl sulfate-polyacryl ... | 1999 | 10387113 |
| molecular characterization of guanosine kinase gene from a facultative alkalophile, exiguobacterium aurantiacum atcc 35652. | guanosine kinase activity was identified in an alkalophile, exiguobacterium aurantiacum atcc 35652, and the gsk gene encoding it was isolated as a homologous fragment of the brevibacterium acetylicum gsk. the cloned chromosomal fragment contained an open reading frame which encodes a polypeptide of 308 amino acids with a molecular mass of 33349 da. the ex. aurantiacum gsk shows strong sequence similarity to b. acetylicum gsk, but possesses five additional amino acids at the c-terminus. an mr 392 ... | 1998 | 9804993 |
| degradation of phenolics, nitrogen-heterocyclics and polynuclear aromatic hydrocarbons in a rotating biological contactor. | the degradation of phenolics, heterocyclics and polynuclear aromatic hydrocarbons (pahs) in a synthetic biomass gasifier wastewater with average cod of 1388 mg/l was studied in a three stage rotating biological contactor (rbc) using the pyrene degrader, exiguobacterium aurantiacum and activated sludge consortia (1:3 v/v). as the organic loading rate (olr) was varied from 3.3 to 14 g/m(2)/d, the cod removal ranged from 63.3% to 92.6%. complete removal of all the constituents was observed at the l ... | 2012 | 22382298 |
| enhanced haloarchaeal oil removal in hypersaline environments via organic nitrogen fertilization and illumination. | hypersaline soil and pond water samples were mixed with 3 % crude oil, some samples were autoclaved to serve as sterile controls; experimental samples were not sterilized. after 6-week incubation at 40 °c under light/dark cycles, the soil microflora consumed 66 %, and after 4 weeks the pond water microflora consumed 63 % of the crude oil. soil samples treated with 3 % casaminoacids lost 89 % of their oil after 6 weeks and water samples lost 86 % after 4 weeks. samples treated with casaminoacids ... | 2012 | 22868892 |
| exiguobacterium himgiriensis sp. nov. a novel member of the genus exiguobacterium, isolated from the indian himalayas. | the taxonomic position of an orange coloured bacterium, strain k22-26(t) isolated from a soil sample was studied using a polyphasic approach. the organism had phenotypic and chemotaxonomic properties consistent with its allocation into the genus exiguobacterium. phylogenetic analysis of the 16s rrna gene sequence showed that strain k22-26(t) belongs to the genus exiguobacterium and was related to exiguobacterium aurantiacum dsm 6208(t) (99.0 %) exiguobacterium mexicanum dsm 16483(t) (98.6 %), ex ... | 2013 | 23229437 |
| exiguobacterium alkaliphilum sp. nov. isolated from alkaline wastewater drained sludge of a beverage factory. | a facultatively anaerobic, alkaliphilic, gram-stain-positive, rod-shaped bacterium, designated strain 12/1(t), isolated from alkaline wastewater drained sludge of a beverage industry facility located near new delhi, india, was subjected to a polyphasic taxonomic study. phylogenetic analysis based on 16s rrna gene sequence comparisons revealed that strain 12/1(t) belonged to the genus exiguobacterium and was most closely related to exiguobacterium aurantiacum dsm 6208(t) (99.46 %), e. aquaticum i ... | 2013 | 23838447 |
| isolation of as-tolerant bacteria and their potentials of reducing as and cd accumulation of edible tissues of vegetables in metal(loid)-contaminated soils. | in this study, three as-tolerant bacteria ralstonia eutropha q2-8, rhizobium tropici q2-13, and exiguobacterium aurantiacum q3-11 were isolated from the rhizosphere and bulk soils of chinese cabbage. the strains were characterized for their production of indole-3-acetic acid (iaa) and siderophores, their effects on soil metal(loid) bioavailability and organic matter content, and their effects on the edible tissue growth and metal(loid) accumulation of chinese cabbage and radish in the metal(loid ... | 2017 | 27839757 |
| isolation characterization and growth of locally isolated hydrocarbonoclastic marine bacteria (eastern algerian coast). | the algerian coastline is being exposed to several types of pollution, including that of hydrocarbons. this environment rich in oil could be the source of proliferation of hydrocarbonoclastic bacteria. the objective of the study is to isolate and identify indigenous bacterial strains from marine waters of two ports in the eastern algerian coast and to test their growth in the presence of hydrocarbons with and without biostimulation throughout the intake of nitrogen and phosphate. results recorde ... | 2017 | 28058612 |
| exiguobacterium aquaticum sp. nov., a new member of the genus exiguobacterium. | a gram positive, motile, short rod shaped, orange pigmented bacterium strain imtb-3094t, isolated from water sample, collected from tikkar tal lake, haryana and was subjected to a detailed polyphasic taxonomic study. strain imtb-3094t, matched with most of the phenotypic and chemotaxonomic properties of the genus exiguobacterium and represented novel species. according to 16s rrna gene sequence analysis, strain imtb-3094t belonged to the genus exiguobacterium. the strain imtb-3094t exhibited hig ... | 2011 | 22058319 |
| characterization of a novel biosurfactant producing pseudomonas koreensis lineage that is endemic to cuatro ciénegas basin. | the aim of this work is the taxonomic characterization of three biosurfactant-producing bacterial isolates from the churince system at cuatro ciénegas basin (ccb) in the mexican state of coahuila, and the study of the possible role of biosurfactant production in their ecology and evolution. we determined that these isolates belong to a pseudomonas koreensis lineage endemic to ccb, using standard taxonomical techniques, phylogenetic analysis of three chromosomal loci and phenotypic characterizati ... | 2011 | 21592712 |
| 16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull. | marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ... | 2010 | 21038149 |
| role of the charged amino acid residues in the cytoplasmic loop between putative transmembrane segments 6 and 7 of na+-atpase of an alkaliphilic bacterium, exiguobacterium aurantiacum. | atpase activity of the membrane-bound na(+)-atpase of an alkaliphilic bacterium, exiguobacterium aurantiacum, was measured in various concentrations of nacl. hill plot analysis showed a hill number of 1.7 with 5.2 mm as the k(0.5) value for na(+). when the site-directed mutagenesis of seven charged amino acid residues in the cytoplasmic loop (l6/7) between putative transmembrane segments 6 and 7 of the enzyme was conducted, all the mutated enzymes exhibited hill numbers close to that of the wild ... | 2009 | 19702882 |
| characterisation of exiguobacterium aurantiacum isolates from blood cultures of six patients. | exiguobacterium spp. are alkaliphilic, halotolerant, non-spore-forming gram-positive bacilli, hitherto uncharacterized from human infections. six isolates of exiguobacterium aurantiacum were obtained from patients with bacteraemia, three of whom had myeloma. all isolates formed orange-yellow pigmented colonies on blood agar, were catalase- and dnase-positive, and grew on nutrient agar at ph 10 and in the presence of nacl 6% w/v. the six isolates were susceptible to all antimicrobial agents teste ... | 2007 | 17645563 |
| exiguobacterium mexicanum sp. nov. and exiguobacterium artemiae sp. nov., isolated from the brine shrimp artemia franciscana. | two gram-positive strains isolated from cysts of the brine shrimp artemia franciscana were subjected to a polyphasic taxonomic analysis. based on 16s rrna gene sequence comparison and composition of isoprenoid quinones, peptidoglycan and fatty acids, these organisms are members of the genus exiguobacterium. both strains showed 95.9% 16s rrna gene sequence similarity to one another. the 16s rrna gene sequences of strain 8n(t) and 9an(t) were 97.5% and 98.9% similar to those of exiguobacterium aur ... | 2006 | 16564954 |
| diversity of alkaliphilic and alkalitolerant bacteria cultivated from decomposing reed rhizomes in a hungarian soda lake. | bacterial communities associated with decomposing rhizomes of phragmites australis were investigated in lake ferto (neusiedlersee, hungary). alkaliphilic and alkalitolerant strains were isolated on cellulose-containing alkaline medium spread with dilutions of scrapings taken from the surface of the decaying plant material. fifty-one strains were grouped by numerical analysis based on physiological tests and biolog sole carbon source utilization data. the strains identified by 16s rdna sequence c ... | 2005 | 16132425 |
| identification of bacteria isolated from an oligotrophic lake with pesticide removal capacities. | we studied the growth and capacities for pesticides removal of bacterial strains isolated from the laguna grande, an oligotrophic lake at the south of spain (archidona, málaga). strains were isolated from water samples amended with 10 and 50 microg/ml of nine pesticides: organochlorinated insecticides (aldrin and lindane), organophosphorous insecticides (dimetoate, methyl-parathion and methidation), s-triazine herbicides (simazine and atrazine), fungicide (captan) and diflubenzuron (1-(-4-chloro ... | 2005 | 15943106 |
| exiguobacterium aestuarii sp. nov. and exiguobacterium marinum sp. nov., isolated from a tidal flat of the yellow sea in korea. | three gram-variable, rod-shaped bacterial strains, tf-16(t), tf-19 and tf-80(t), were isolated from a tidal flat of daepo beach (yellow sea) near mokpo city, korea, and their taxonomic positions were investigated by a polyphasic approach. these isolates grew optimally in the presence of 2 % nacl and at 30 degrees c. their peptidoglycan types were based on l-lys-gly. the predominant menaquinone detected in the three strains was mk-7. the three strains contained large amounts of the branched fatty ... | 2005 | 15774680 |
| cloning, sequencing and functional expression in escherichia coli of the gene for a p-type na(+)-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | cloning and sequencing of the gene encoding a p-type na(+)-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, were conducted. the structural gene was composed of 2628 nucleotides. the deduced amino acid sequence (876 amino acid residues; mr, 96,664) suggested that the enzyme possesses 10 membrane-spanning regions. when the amino acid sequences of the four putative membrane regions, m4, m5, m6 and m8, of bl77/1 atpase were aligned with those of fungal na(+)-atpase, na(+ ... | 2005 | 15715958 |
| denaturing gradient gel electrophoresis analysis to study bacterial community structure in pockets of periodontitis patients. | bacteria are involved in the onset and progression of periodontitis. a promising molecular technique, denaturing gradient gel electrophoresis (dgge), to study microbial population dynamics in the subgingival pocket is presented. twenty-three samples were taken from the subgingival pockets of nine patients and six healthy family members. from four periodontitis patients, 12 samples were evaluated before, 1 day after and 3 months after treatment. part of the 16s rrna gene of all bacteria was ampli ... | 2003 | 12588461 |
| reduction of cr(vi) and bioaccumulation of chromium by gram positive and gram negative microorganisms not previously exposed to cr-stress. | resistance to cr(vi) is usually associated with its cellular exclusion, precluding enrichment techniques for the isolation of organisms accumulating cr(vi) via bioreduction to insoluble cr(iii). a technique was developed to screen for potential cr(vi) reduction in approx. 2000 isolates from a coastal environment, based on the non-specific reduction of selenite and tellurite to se0 and te0, and reduction of tetrazolium blue to insoluble blue formazan. the most promising strains were further scree ... | 2002 | 12164635 |