| the sodium cycle: a novel type of bacterial energetics. | the progress of bioenergetic studies on the role of na+ in bacteria is reviewed. experiments performed over the past decade on several bacterial species of quite different taxonomic positions show that na+ can, under certain conditions, substitute for h+ as the coupling ion. various primary na+ pumps (delta mu na+ generators) are described, i.e., na+ -motive decarboxylases, nadh-quinone reductase, terminal oxidase, and atpase. the delta mu na+ formed is shown to be consumed by na+ driven atp-syn ... | 1989 | 2687258 |
| phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera caryophanon, exiguobacterium, kurthia, and planococcus. | the 16s rrna gene sequences of "bacillus aminovorans" and several species considered to be phylogenetically related to the group 2 bacilli of ash et al. (c. ash, j. a. e. farrow, s. wallbanks, and m. d. collins, lett. appl. microbiol. 13:202-206, 1991) were determined. a comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa (the genera caryophanon, exiguobacterium, kurthia, planococcus), form a phylogenetically disti ... | 1994 | 8123563 |
| phosphorylation of guanosine using guanosine-inosine kinase from exiguobacterium acetylicum coupled with atp regeneration. | guanosine 5'-monophosphate (5'-gmp) and inosine 5'-monophosphate (5'-imp) are widely used as flavor enhancers. recently, a novel process for 5'-imp production by phosphorylation of inosine using guanosine-inosine kinase coupled with atp regeneration was reported. in this study, we demonstrated the practical possibility of producing 5'-gmp by phosphorylation of guanosine using a guanosine-inosine kinase from exiguobacterium acetylicum coupled with atp regeneration. | 2000 | 11129609 |
| class ii broad-spectrum mercury resistance transposons in gram-positive bacteria from natural environments. | we have studied the mechanisms of the horizontal dissemination of a broad-spectrum mercury resistance determinant among bacillus and related species. this mer determinant was first described in bacillus cereus rc607 from boston harbor, usa, and was then found in various bacillus and related species in japan, russia and england. we have shown that the mer determinant can either be located at the chromosome, or on a plasmid in the bacillus species, and is carried by class ii mercury resistance tra ... | 2001 | 11446519 |
| possible involvement of a single histidine residue in the p-type na+-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | effect of various inhibitors on the p-type na+-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, was examined. the atpase was extremely sensitive to p-chloromercuriphenylsulfonic acid, a modifier of sh-group. the enzyme was also sensitive to diethylpyrocarbonate, and analysis of the inhibition kinetics by the drug indicated that modification of a single histidine residue per atpase molecule was sufficient to inactivate the enzyme. | 2001 | 11685505 |
| reduction of cr(vi) and bioaccumulation of chromium by gram positive and gram negative microorganisms not previously exposed to cr-stress. | resistance to cr(vi) is usually associated with its cellular exclusion, precluding enrichment techniques for the isolation of organisms accumulating cr(vi) via bioreduction to insoluble cr(iii). a technique was developed to screen for potential cr(vi) reduction in approx. 2000 isolates from a coastal environment, based on the non-specific reduction of selenite and tellurite to se0 and te0, and reduction of tetrazolium blue to insoluble blue formazan. the most promising strains were further scree ... | 2002 | 12164635 |
| [mercury-resistant bacteria from permafrost sediments and prospects for their use in comparative studies of mercury resistance determinants]. | mercury-resistant bacteria were isolated from permafrost sediments of kolyma lowland and canada existing over five thousand to two million years. their content was shown to vary within the range 0.001-2.9% and to depend on the amount of mercury in sampling sites (coefficient of correlation 0.75). a collection of mercury-resistant bacterial strains was created. in this collection, various representatives of both gram-positive bacteria (bacillus, exiguobacterium, micrococcus, arthrobacter) and gra ... | 2002 | 12500685 |
| denaturing gradient gel electrophoresis analysis to study bacterial community structure in pockets of periodontitis patients. | bacteria are involved in the onset and progression of periodontitis. a promising molecular technique, denaturing gradient gel electrophoresis (dgge), to study microbial population dynamics in the subgingival pocket is presented. twenty-three samples were taken from the subgingival pockets of nine patients and six healthy family members. from four periodontitis patients, 12 samples were evaluated before, 1 day after and 3 months after treatment. part of the 16s rrna gene of all bacteria was ampli ... | 2003 | 12588461 |
| isolation and characterization of arsenate-reducing bacteria from arsenic-contaminated sites in new zealand. | two environmental sites in new zealand were sampled (e.g., water and sediment) for bacterial isolates that could use either arsenite as an electron donor or arsenate as an electron acceptor under aerobic and anaerobic growth conditions, respectively. these two sites were subjected to widespread arsenic contamination from mine tailings generated from historic gold mining activities or from geothermal effluent. no bacteria were isolated from these sites that could utilize arsenite or arsenate unde ... | 2004 | 15060729 |
| bacterial diversity in spent mushroom compost assessed by amplified rdna restriction analysis and sequencing of cultivated isolates. | spent mushroom compost (smc) is the residual by-product of commercial agaricus spp. cultivation, and it is mainly composed of a thermally treated cereal straw/animal manure mixture colonized by the fungal biomass. research on the valorization of this material is mainly focusing on its use as soil conditioner and plant fertilizer. an investigation of the bacterial diversity in smc was performed using molecular techniques in order to reveal the origin of smc microflora and its potential effect on ... | 2004 | 15612633 |
| identification of bacteria isolated from an oligotrophic lake with pesticide removal capacities. | we studied the growth and capacities for pesticides removal of bacterial strains isolated from the laguna grande, an oligotrophic lake at the south of spain (archidona, málaga). strains were isolated from water samples amended with 10 and 50 microg/ml of nine pesticides: organochlorinated insecticides (aldrin and lindane), organophosphorous insecticides (dimetoate, methyl-parathion and methidation), s-triazine herbicides (simazine and atrazine), fungicide (captan) and diflubenzuron (1-(-4-chloro ... | 2005 | 15943106 |
| diversity of alkaliphilic and alkalitolerant bacteria cultivated from decomposing reed rhizomes in a hungarian soda lake. | bacterial communities associated with decomposing rhizomes of phragmites australis were investigated in lake ferto (neusiedlersee, hungary). alkaliphilic and alkalitolerant strains were isolated on cellulose-containing alkaline medium spread with dilutions of scrapings taken from the surface of the decaying plant material. fifty-one strains were grouped by numerical analysis based on physiological tests and biolog sole carbon source utilization data. the strains identified by 16s rdna sequence c ... | 2005 | 16132425 |
| [effect of pentachlorophenol (pcp) on bacterial community of an aerobic granular sludge estimated by t-rflp]. | effect of pentachlorophenol (pcp) on cod and ammonium removal rates was investigated, and also changes of bacterial community within aerobic granular sludge were monitored by terminal restriction fragment length polymorphism (t-rflp) technique. compared with the removal rate of cod, the rate of ammonium removal is more sensitive to pcp. as the ammonium and cod removal rates decreased consistently, the bacterial genera within the aerobic granular sludge also decrease with the increase of pcp conc ... | 2006 | 16768009 |
| phylogenetic analysis of a biofilm bacterial population in a water pipeline in the gulf of mexico. | the aim of this study was to assess the bacterial diversity associated with a corrosive biofilm in a steel pipeline from the gulf of mexico used to inject marine water into the oil reservoir. several aerobic and heterotrophic bacteria were isolated and identified by 16s rrna gene sequence analysis. metagenomic dna was also extracted to perform a denaturing gradient gel electrophoresis analysis of ribosomal genes and to construct a 16s rrna gene metagenomic library. denaturing gradient gel electr ... | 2006 | 16958915 |
| culturable and vbnc vibrio cholerae: interactions with chironomid egg masses and their bacterial population. | vibrio cholerae, the etiologic agent of cholera, is autochthonous to various aquatic environments. recently, it was found that chironomid (nonbiting midges) egg masses serve as a reservoir for the cholera bacterium and that flying chironomid adults are possible windborne carriers of v. cholerae non-o1 non-o139. chironomids are the most widely distributed insect in freshwater. females deposit egg masses at the water's edge, and each egg mass contains eggs embedded in a gelatinous matrix. hemagglu ... | 2007 | 17186156 |
| isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments. | in assessing the bacterial populations present in spacecraft assembly, spacecraft test, and launch preparation facilities, extremophilic bacteria (requiring severe conditions for growth) and extremotolerant bacteria (tolerant to extreme conditions) were isolated. several cultivation approaches were employed to select for and identify bacteria that not only survive the nutrient-limiting conditions of clean room environments but can also withstand even more inhospitable environmental stresses. due ... | 2007 | 17308177 |
| molecular identification of exiguobacterium acetylicum as the aetiological agent of bacteraemia. | a case of catheter-related bacteraemia caused by exiguobacterium acetylicum is reported in an elderly patient. the availability of sequence-based methods facilitated rapid identification and expanded the spectrum of diseases attributed to coryneform bacteria and specifically to the genus exiguobacterium. | 2007 | 17374901 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| [screening and characterization of marine bacteria with antibacterial and cytotoxic activities, and existence of pks i and nrps genes in bioactive strains]. | antibacterial and cytotoxic activities were screened for marine bacteria which have been isolated from organism, sediments and seawater in china coastal area. the results showed that 42 isolates had antimicrobial activity and 12 isolates had cytotoxicity. molecular phylogenetic analysis of marine bacteria with cytotoxicity based on 16s rrna sequences indicated that they were belong to the genera aerococcus, agrobacterium, alteromona, bacillus, exiguobacterium, paracoccus, pseudoalteromons, rhein ... | 2007 | 17552225 |
| stability of microbial communities in goat milk during a lactation year: molecular approaches. | the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ... | 2007 | 17604934 |
| cultivable bacterial diversity of alkaline lonar lake, india. | aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season, january 2002 from alkaline lonar lake, india, having ph 10.5. the total number of microorganisms in the sediment and water samples was found to be 10(2)-10(6) cfu g(-1) and 10(2)-10(4) cfu ml(-1), respectively. one hundred and ninety-six strains were isolated using different enrichment media. to study the bacterial diversity of lonar lake and to select the bacterial stra ... | 2008 | 17604989 |
| effect of low temperature and culture media on the growth and freeze-thawing tolerance of exiguobacterium strains. | bacteria of the genus exiguobacterium have been repeatedly isolated from ancient permafrost sediments of the kolyma lowland of northeast eurasia. here we report that the siberian permafrost isolates exiguobacterium sibiricum 255-15, e. sibiricum 7-3, exiguobacterium undae 190-11 and e. sp. 5138, as well as exiguobacterium antarcticum dsm 14480, isolated from a microbial mat sample of lake fryxell (mcmurdo dry valleys, antarctica), were able to grow at temperatures ranging from -6 to 40 degrees c ... | 2007 | 17382311 |
| molecular identification of environmental bacteria in indoor air in the domestic home: description of a new species of exiguobacterium. | the quality of indoor air in terms of its bioaerosol composition with microorganisms is important due to its potential aetiological role in development of conditions such as sick building syndrome. hence, laboratory identification of bacteriological components in any bioaerosol from buildings may help elucidate the role of such organisms in disease states, particularly allergy-related conditions. a molecular method was developed employing universal or "broad-range" eubacterial pcr to help identi ... | 2007 | 17365082 |
| comparison of in vitro cr(vi) reduction by cfes of chromate resistant bacteria isolated from chromate contaminated soil. | chromate resistant and reducing strains were isolated from chromium contaminated soil and identified as bacillus sp. (kch2 and kch3), leucobacter sp. (kch4) and exiguobacterium sp. (kch5). kch3 and kch4 showed higher cr(vi) tolerance (2 mm) and cr(vi) reduction (1.5 mm) than kch5 (1.5 mm and 0.75 mm, respectively). cr(vi) reduction by cfes of kch3 and kch4 showed nad(p)h dependence, optimum activity at ph 5.5, low k(m) (45-55 microm) and substrate inhibition by cr(vi) (>75 microm), whereas that ... | 2008 | 17920879 |
| isolation of a psychrotrophic exiguobacterium sp. skpb5 (mtcc 7803) and characterization of its alkaline protease. | out of nine psychrotrophic bacterial strains isolated from cold environments of the western himalayas, skpb5 was selected for protease purification and characterization because it had the largest zone of clearance on plate assay. on the basis of the phenotypic and biochemical characterization and 16s rrna gene-sequencing studies, isolate was identified as exiguobacterium sp. skpb5. the protease was purified near to homogeneity with a purification fold of 7.1, and its molecular weight was determi ... | 2007 | 17294327 |
| [phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china]. | the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ... | 2007 | 17944352 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| exiguobacterium profundum sp. nov., a moderately thermophilic, lactic acid-producing bacterium isolated from a deep-sea hydrothermal vent. | a facultatively anaerobic, halotolerant, moderately thermophilic and non-sporulating bacterium, designated strain 10c(t), was isolated from deep-sea hydrothermal vent samples collected on the 13 degrees n east pacific rise at a depth of approximately 2600 m. cells of strain 10c(t) were gram-positive, motile rods, and grew optimally at 45 degrees c (range 12-49 degrees c), ph 7.0 (range ph 5.5-9.5) and 0-2 % nacl (range 0-11 %). (+)-l-lactate was the main organic acid detected from carbohydrate f ... | 2007 | 17267965 |
| isolation, gene detection and solvent tolerance of benzene, toluene and xylene degrading bacteria from nearshore surface water and pacific ocean sediment. | btx (benzene, toluene and xylene) degrading bacteria were isolated from pacific ocean sediment and nearshore surface water. in the seawater near a ferry dock, degrading bacteria of a relatively wide diversity were detected, including species of pseudomonas, rhodococcus, exiguobacterium and bacillus; while species of bacillus only have been detected from the deep-sea sediment. most of the isolates showed degradation to more than one compound. generally better growth was obtained with p-xylene and ... | 2008 | 18286224 |
| relationship between the size of the bottleneck 15 a from iron in the main channel and the reactivity of catalase corresponding to the molecular size of substrates. | a catalase that exhibits a high level of activity and a rapid reaction with organic peroxides has been purified from exiguobacterium oxidotolerans t-2-2t (ekta catalase). the amino acid sequence of ekta catalase revealed that it is a novel clade 1 catalase. amino acid residues in the active site around the protoheme are conserved in the primary structure of ekta catalase. although the general interactions of molecules larger than hydrogen peroxide with catalases are strongly inhibited because of ... | 2007 | 17198371 |
| biological treatment of tannery wastewater by using salt-tolerant bacterial strains. | abstract: | 2008 | 18445252 |
| ribosomal rna-based analysis of the bacterial flora from the conjunctivae of cattle with bovine keratoconjunctivitis (bkc). | bovine keratoconjunctivitis (bkc), colloquially referred to as 'pinkeye', is a disease affecting cattle worldwide; it costs cattle producers millions of dollars in economic loss annually. while moraxella spp. are the primary etiologic agent of pinkeye, surveys of flora from the conjunctivae of livestock from around the world have indicated that a variety of bacterial commensals occupy this niche. we used molecular biology-based methods to determine the composition of bacterial flora in the conju ... | 2008 | 18513895 |
| characterization of depth-related microbial communities in lake sediment by denaturing gradient gel electrophoresis of amplified 16s rrna fragments. | the characterization of microbial communities of different depth sediment samples was examined by a culture-independent method and compared with physicochemical parameters, those are organic matter (om), total nitrogen (tn), total phosphorus (tp), ph and redox potential (eh). total genomic dna was extracted from samples derived from different depths. after they were amplified with the gc-341f/907r primer sets of partial bacterial 16s rrna genes, the products were separated by denaturing gradient ... | 2008 | 18574965 |
| exiguobacterium indicum sp. nov., a psychrophilic bacterium from the hamta glacier of the himalayan mountain ranges of india. | strain hhs 31(t), a gram-positive, motile, rod-shaped, non-spore-forming, alkaliphilic bacterium, was isolated from the melt water of a glacier. phenotypic and chemotaxonomic characteristics indicate that strain hhs 31(t) is related to species of the genus exiguobacterium. the 16s rrna gene sequence similarities between hhs 31(t) and strains of known species confirm that it is closely related to members of the genus exiguobacterium (93-99 %) and that it exhibits >97 % similarity with exiguobacte ... | 2006 | 17158975 |
| multi-locus real-time pcr for quantitation of bacteria in the environment reveals exiguobacterium to be prevalent in permafrost. | we developed a multi-locus quantitative pcr approach to minimize problems of precision, sensitivity and primer specificity for quantifying a targeted microbial group in nature. this approach also avoids a systematic error in population quantitation when 16s rrna genes are used because of copy number heterogeneity. specific primers were designed to assess the abundance of psychrotrophic and mesophilic exiguobacterium spp. that excluded the thermophilic members of the genus. the chosen primers tar ... | 2007 | 17156079 |
| proteomic analysis of cold adaptation in a siberian permafrost bacterium--exiguobacterium sibiricum 255-15 by two-dimensional liquid separation coupled with mass spectrometry. | bacterial cold adaptation in exiguobacterium sibiricum 255-15 was studied on a proteomic scale using a 2-d liquid phase separation coupled with ms technology. whole-cell lysates of e. sibiricum 255-15 grown at 4 degrees c and 25 degrees c were first fractionated according to pi by chromatofocusing (cf), and further separated based on hydrophobicity by nonporous silica rp hplc (nps-rp-hplc) which was on-line coupled with an esi-tof ms for intact protein m(r) measurement and quantitative interlysa ... | 2006 | 16955517 |
| coordinate action of exiguobacterial oxidoreductive enzymes in biodegradation of reactive yellow 84a dye. | a novel bacterial species identified as exiguobacterium sp. rd3 degraded the diazo dye reactive yellow 84a (50 mg l(-1)) within 48 h at static condition, at 30 degrees c and ph 7. lower salinity conditions were found to be favorable for growth and decolorization. enzymatic activities of an h(2)o(2) independent oxidase along with laccase and an azoreductase suggest their prominent role during the decolorization of reactive yellow 84a. presence of an h(2)o(2) independent oxidase in exiguobacterium ... | 2009 | 18807200 |
| potential misidentification of a new exiguobacterium sp. as oerskovia xanthineolytica isolated from blood culture. | | 2006 | 16872001 |
| bioinformatic and partial functional analysis of pespa and pespb, two plasmids from exiguobacterium arabatum sp. nov. rfl1109. | the complete nucleotide sequences of two plasmids from exiguobacterium arabatum sp. nov. rfl1109, pespa (4563bp) and pespb (38,945bp), have been determined. five orfs were identified in the pespa plasmid, and putative functions were assigned to two of them. using deletion mapping approach, the rep-independent replication region of pespa, which functions in bacillus subtilis, was localized within a 0.6kb dna region. analysis of the pespb sequence revealed 42 orfs. from these, function of two gene ... | 2009 | 18848579 |
| bacterial community in ancient siberian permafrost as characterized by culture and culture-independent methods. | the microbial composition of ancient permafrost sediments from the kolyma lowland of northeast eurasia was examined through culture and culture-independent approaches. these sediments have been continuously frozen for 5,000 to 2-3 million years. a total of 265 bacteria 16s rrna gene sequences were amplified from the permafrost total-community genomic dna and screened by amplified ribosomal 16s rrna restriction analysis. members of three major lineages were found: gamma-proteobacteria (mostly xan ... | 2006 | 16805696 |
| carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a canadian mill. | forty-one bacterial strains isolated from the headbox water of two machines in a canadian paper mill were associated with the genera asticcacaulis, acidovorax, bacillus, exiguobacterium, hydrogenophaga, pseudomonas, pseudoxanthomonas, staphylococcus, stenotrophomonas based on the sequence of their 16s rrna genes. the metabolic profile of these strains were determined using biolog ecoplate, and the bacteria were divided into four metabolic groups. metabolic profiles of the bacterial communities c ... | 2009 | 19137341 |
| [variation of bacterial community composition in the outbreak and decline of microcystis spp. bloom in lake xuanwu]. | water samples were collected in the outbreak and decline of microcystis spp. bloom in three different lake areas of lake xuanwu for studying the bacterial community composition during the bloom to provide clew for understanding the dynamics of water bloom. traditional microbiological methods were used for culturable bacterial analysis, while the amplified products of variable v3 region of bacterial 16s rdna were used for bacterial community dna finger print by using denaturing gradient gel elect ... | 2008 | 19143401 |
| identification and characteristics of a novel salt-tolerant exiguobacterium sp. for azo dyes decolorization. | a salt-tolerant bacterium was isolated from the surface soil of a pharmaceutical factory, which could efficiently decolorize azo dyes. the strain was identified as exiguobacterium sp. according to its morphological characteristics and 16s rrna gene sequence analysis. decolorization of x-3b with resting cells of this strain, which were catalyzed by redox mediator (anthraquinone), was studied, and the conditions were optimized. for color removal and cells growth, the optimal inoculation amount, ph ... | 2009 | 19198765 |
| exiguobacterium mexicanum sp. nov. and exiguobacterium artemiae sp. nov., isolated from the brine shrimp artemia franciscana. | two gram-positive strains isolated from cysts of the brine shrimp artemia franciscana were subjected to a polyphasic taxonomic analysis. based on 16s rrna gene sequence comparison and composition of isoprenoid quinones, peptidoglycan and fatty acids, these organisms are members of the genus exiguobacterium. both strains showed 95.9% 16s rrna gene sequence similarity to one another. the 16s rrna gene sequences of strain 8n(t) and 9an(t) were 97.5% and 98.9% similar to those of exiguobacterium aur ... | 2006 | 16564954 |
| the exiguobacterium genus: biodiversity and biogeography. | bacteria of the genus exiguobacterium are low g + c, gram-positive facultative anaerobes that have been repeatedly isolated from ancient siberian permafrost. in addition, exiguobacterium spp. have been isolated from markedly diverse sources, including greenland glacial ice, hot springs at yellowstone national park, the rhizosphere of plants, and the environment of food processing plants. strains of this hereto little known bacterium that have been retrieved from such different (and often extreme ... | 2009 | 19381755 |
| characterization of exiguobacterium isolates from the siberian permafrost. description of exiguobacterium sibiricum sp. nov. | three gram-positive bacterial strains, 7-3, 255-15 and 190-11, previously isolated from siberian permafrost, were characterized and taxonomically classified. these microorganisms are rod-shaped, facultative aerobic, motile with peritrichous flagella and their growth ranges are from -2.5 to 40 degrees c. the chemotaxonomic markers indicated that the three strains belong to the genus exiguobacterium. their peptidoglycan type was a3alpha l-lys-gly. the predominant menaquinone detected in all three ... | 2006 | 16489412 |
| characterization of potential stress responses in ancient siberian permafrost psychroactive bacteria. | past studies of cold-acclimated bacteria have focused primarily on organisms not capable of sub-zero growth. siberian permafrost isolates exiguobacterium sp. 255-15 and psychrobacter sp. 273-4, which grow at subzero temperatures, were used to study cold-acclimated physiology. changes in membrane composition and exopolysaccharides were defined as a function of growth at 24, 4 and -2.5 degrees c in the presence and absence of 5% nacl. as expected, there was a decrease in fatty acid saturation and ... | 2005 | 16329933 |
| efficient decomposition of shrimp shell waste using bacillus cereus and exiguobacterium acetylicum. | two bacterial cultures were isolated and tested for degradation of shrimp shell waste. according to morphological examination, physiological tests, and applied molecular techniques, isolates were identified as bacillus cereus and exiguobacterium acetylicum. both strains were cultivated separately in flasks with 100 ml of shrimp shell waste broth (3% of washed, dried and ground shrimp shell waste in tap water, ph 7.0) at 37 degrees c. at determined periods of time, deproteinization and deminerali ... | 2009 | 19471983 |
| putative transposases conserved in exiguobacterium isolates from ancient siberian permafrost and from contemporary surface habitats. | gram-positive bacteria of the genus exiguobacterium have been repeatedly isolated from siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in lake fryxell (antarctic), surface water, and food-processing environments. however, there is currently no information on genomic diversity of this microorganism or on the physiological strategies that have allowed its survival under prolonged fr ... | 2005 | 16269730 |
| biological activity studies on metal complexes of novel tridentate schiff base ligand. spectroscopic and thermal characterization. | metal complexes of novel schiff base (hl) ligand, prepared via condensation of 4-aminoantipyrine and 2-aminophenol, are prepared. the ligand is characterized based on elemental analysis, mass, ir and (1)h nmr spectra. metal complexes are reported and characterized based on elemental analyses, ir, (1)h nmr, solid reflectance, magnetic moment, molar conductance, esr spectra and thermal analyses (tg, dtg and dta). from the elemental analyses, 1:1 [m]:[ligand] complexes are prepared with the general ... | 2009 | 19758728 |
| molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques. | polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ... | 2010 | 20018377 |
| isolation of uv-b resistant bacteria from two high altitude andean lakes (4,400 m) with saline and non saline conditions. | laguna (l.) negra and l. verde are high altitude andean lakes located at the 4,400 m altitude in the andean desert (puna) in the argentine northwest. both lakes are exposed to extreme weather conditions but differ in salinity contents (salinity 6.7% for l. negra and 0.27% for l. verde). the aim of this work was to isolate ultraviolet b fraction (uv-b) resistant bacteria under uv-stress in order to determine, a possible connection, between resistance to uv-b and tolerance to salinity. dna damage ... | 2009 | 20118609 |
| exiguobacterium aestuarii sp. nov. and exiguobacterium marinum sp. nov., isolated from a tidal flat of the yellow sea in korea. | three gram-variable, rod-shaped bacterial strains, tf-16(t), tf-19 and tf-80(t), were isolated from a tidal flat of daepo beach (yellow sea) near mokpo city, korea, and their taxonomic positions were investigated by a polyphasic approach. these isolates grew optimally in the presence of 2 % nacl and at 30 degrees c. their peptidoglycan types were based on l-lys-gly. the predominant menaquinone detected in the three strains was mk-7. the three strains contained large amounts of the branched fatty ... | 2005 | 15774680 |
| comparative assessment of selenite (seiv) detoxification to elemental selenium (se0) by bacillus sp. | bacillus licheniformis, b. subtilis, b. cereus, bacillus pumilus and exiguobacterium sp., which were resistant up to 20 mg na(2)seo(3)/ml in nutrient broth and 40 mg/ml on nutrient agar plates, were isolated from contaminated soil and water. they grew from 25 to 45 degrees c and ph 5 to 9. they had multiple metal and antibiotic resistances. all strains reduced selenite (seiv) to elemental selenium (se0) aerobically with a maximum reduction of 97% by b. pumilus after 144 h with na(2)seo(3) at 500 ... | 2010 | 20455073 |
| cloning, sequencing and functional expression in escherichia coli of the gene for a p-type na(+)-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | cloning and sequencing of the gene encoding a p-type na(+)-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, were conducted. the structural gene was composed of 2628 nucleotides. the deduced amino acid sequence (876 amino acid residues; mr, 96,664) suggested that the enzyme possesses 10 membrane-spanning regions. when the amino acid sequences of the four putative membrane regions, m4, m5, m6 and m8, of bl77/1 atpase were aligned with those of fungal na(+)-atpase, na(+ ... | 2005 | 15715958 |
| diversity of the predominant spoilage bacteria in water-boiled salted duck during storage. | the spoilage microbiota in water-boiled salted duck during storage at 4 degrees c was determined using culture-dependent and independent methods. analysis of the denaturing gradient gel electrophoresis (dgge) patterns of pcr amplicons targeting the v3 region of the 16s rdna and sequencing of the bands allowed profiling of the microbiota present in the duck. community dna extracts were prepared directly from water-boiled salted duck and from culturable bacterial fractions harvested from both mrs ... | 2010 | 20629890 |
| exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity. | a novel alkaliphile was isolated from a drain of a fish processing plant. the isolate grew at a ph range of 7-10. cells were gram-positive, facultatively aerobic, motile rods with peritrichous flagella. colonies were orange or yellow in colour. catalase and oxidase reactions were positive. the isolate grew in 0-12 % nacl but not above 15 % nacl. its cell extract exhibited 567 times higher catalase activity than an escherichia coli cell extract. the major cellular fatty acids were iso-c(13 : 0), ... | 2004 | 15545426 |
| predicted bacteriorhodopsin from exiguobacterium sibiricum is a functional proton pump. | the predicted exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient siberian permafrost sample. the protein bacteriorhodopsin from exiguobacterium sibiricum (esr) encoded by this gene was expressed in escherichia coli membrane. esr bound all-trans-retinal and displayed an absorbance maximum at 534nm without dark adaptation. the esr photocycle is characterized by fast formation of an m intermediate and the presence of a significant amount of an o intermediate. proteoliposom ... | 2010 | 20831870 |
| diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania. | aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ... | 2010 | 20941581 |
| structure of a putative ntp pyrophosphohydrolase: yp_001813558.1 from exiguobacterium sibiricum 255-15. | the crystal structure of a putative ntpase, yp_001813558.1 from exiguobacterium sibiricum 255-15 (pf09934, duf2166) was determined to 1.78 å resolution. yp_001813558.1 and its homologs (dimeric dutpases, mazg proteins and hise-encoded phosphoribosyl atp pyrophosphohydrolases) form a superfamily of all-α-helical ntp pyrophosphatases. in dimeric dutpase-like proteins, a central four-helix bundle forms the active site. however, in yp_001813558.1, an unexpected intertwined swapping of two of the hel ... | 2010 | 20944217 |
| 16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull. | marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ... | 2010 | 21038149 |
| isolation, characterization, and use for plant growth promotion under salt stress, of acc deaminase-producing halotolerant bacteria derived from coastal soil. | in total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the yellow sea, near the city of incheon in the republic of korea. all of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (iaa), nitrogen fixation, phosphorus (p) and zinc (zn) solubilization, thiosulfate (s2o3) oxidation, the production of ammonia ... | 2010 | 21124065 |
| role of bacterial isolates in enhancing the bud induction in the industrially important red alga gracilaria dura. | plant growth depends on the integration of environmental cues, nitrogen fixation and phytohormone-signaling pathways. the growth and development of gracilaria dura was significantly influenced by the association of bacterial isolates. the putative bud-inducing epiphytic exiguobacterium homiense and endophytic bacillus pumilus, bacillus licheniformis were examined for their ability to fix nitrogen and produce indole-3-acetic acid (iaa). these bacterial isolates were identified to the species leve ... | 2011 | 21255055 |
| cloning and overexpression of the exiguobacterium sp. f42 gene encoding a new short chain dehydrogenase, which catalyzes the stereoselective reduction of ethyl 3-oxo-3-(2-thienyl)propanoate to ethyl (s)-3-hydroxy-3-(2-thienyl)propanoate. | exiguobacterium sp. f42 was screened as a producer of an enzyme catalyzing the nadph-dependent stereoselective reduction of ethyl 3-oxo-3-(2-thienyl)propanoate (kees) to ethyl (s)-3-hydroxy-3-(2-thienyl)propanoate ((s)-hees). (s)-hees is a key intermediate for the synthesis of (s)-duloxetine, a potent inhibitor of the serotonin and norepinephrine uptake carriers. the responsible enzyme (kees reductase) was partially purified, and the gene encoding kees reductase was cloned and sequenced via an i ... | 2004 | 15277752 |
| the role of bacteria in oral cancer. | despite the widening interest in the possible association between bacteria and different stages of cancer development, our knowledge in its relation to oral cancers remains inadequate. the aim of this review article is to derive a better understanding on the role of various micro-organisms in the etiogenesis of oral cancers through all the available data on the pubmed. different bacteria have been proposed to induce carcinogenesis either through induction of chronic inflammation or by interferen ... | 2010 | 21584217 |
| characterization of a novel biosurfactant producing pseudomonas koreensis lineage that is endemic to cuatro ciénegas basin. | the aim of this work is the taxonomic characterization of three biosurfactant-producing bacterial isolates from the churince system at cuatro ciénegas basin (ccb) in the mexican state of coahuila, and the study of the possible role of biosurfactant production in their ecology and evolution. we determined that these isolates belong to a pseudomonas koreensis lineage endemic to ccb, using standard taxonomical techniques, phylogenetic analysis of three chromosomal loci and phenotypic characterizati ... | 2011 | 21592712 |
| culture-dependent and -independent molecular analysis of the bacterial community within uranium ore. | the bacterial community structure within a uranium ore was investigated using culture-dependent and -independent clone library analysis and denaturing gradient gel electrophoresis of 16s rrna genes. the major aerobic heterotrophic bacteria were isolated and identified, and their resistance to uranium and other heavy metals was characterized. together with near neutral ph, moderate organic carbon content, elevated u and other heavy metals (v, ni, mn, cu, etc.), the ore showed high microbial count ... | 2011 | 21656801 |
| exiguobacterium undae sp. nov. and exiguobacterium antarcticum sp. nov. | four orange-pigmented strains from pond water (l1-l4) have been subjected to polyphasic taxonomic analyses. on the basis of ribotype analysis and fourier-transform infrared spectroscopy, these strains form a genomically highly related group. 16s rdna sequence analysis revealed 98.8% similarity between the 16s rdna sequences of strains l2t and h2t, isolated previously from a microbial mat from lake fryxell, antarctica. dna-dna reassociation values indicated the presence of two genomic clusters. w ... | 2002 | 12148624 |
| Bacterial assisted phytoremediation for enhanced degradation of highly sulfonated diazo reactive dye. | PURPOSE: Phytoremediation is the exploitation of plants and their rhizospheric microorganisms for pollutants treatment like textile dyes, which are toxic, carcinogenic and mutagenic from the effluent. The purpose of this work was to explore a naturally found plant and bacterial synergism to achieve an enhanced degradation of Remazol Black B dye (RBB). METHODS: In vitro cultures of Zinnia angustifolia were obtaine ... | 2011 | 22161298 |
| evaluation of bacterial contaminants found on unused paper towels and possible postcontamination after handwashing: a pilot study. | background: bacterial contamination is a concern in the pulp and paper industry. not only is the machinery contaminated but also can be the end-paper products. bacterial transmission from unused paper towels to hands and surfaces is not well documented. methods: the culturable bacterial community of 6 different unused paper towel brands was determined by culture methods and by sequencing the 16s ribosomal dna of bacterial contaminants. next, we investigated the possible airborne and direct conta ... | 2011 | 22177666 |
| characterization of a p-type na+-atpase of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, possesses a p-type na(+)-stimulated atpase in the membrane (koyama, n. (1999) curr. microbiol. 39, 27-30). in this study, we attempted to purify and characterize the enzyme. the atpase appears to consist of a single polypeptide with an apparent molecular mass of 100 kda. the enzyme exhibited an optimum ph for activity at around 9. the enzyme was strongly inhibited by vanadate (50% inhibition observed at 3 microm) and forms an ac ... | 2000 | 10799538 |
| identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis candidates. | to describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ... | 2012 | 22074685 |
| purification and properties of a novel azide-sensitive atpase of exiguobacterium aurantiacum. | exiguobacterium aurantiacum bl77/1 possesses at least two distinct membrane-bound atpases. one of them was solubilized with decanoyl n-methylglucamide, a non-ionic detergent, and purified by successive chromatography on deae-sepharose and hydroxyapatite. the purified atpase appears to consist of a single polypeptide component with an apparent molecular mass of 54 kda. among the triphosphates of various nucleosides tested, atp was the best substrate. the enzyme exhibited a km of 0.5 mm for atp an ... | 2000 | 10763752 |
| presence of na+-stimulated p-type atpase in the membrane of a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum. | it was found that a facultatively anaerobic alkaliphile, exiguobacterium aurantiacum, possesses a membrane-bound atpase, which was activated specifically by na+. the na+-stimulated atpase activity reached a maximum value at 200 mm nacl. in the presence of 200 mm nacl, the activity was drastically reduced by vanadate, a potent inhibitor of p-type atpase, with a half-maximal inhibition at 1 microm. incubation of the membranes with [gamma-32p]atp followed by acidic lithium dodecyl sulfate-polyacryl ... | 1999 | 10387113 |
| molecular characterization of guanosine kinase gene from a facultative alkalophile, exiguobacterium aurantiacum atcc 35652. | guanosine kinase activity was identified in an alkalophile, exiguobacterium aurantiacum atcc 35652, and the gsk gene encoding it was isolated as a homologous fragment of the brevibacterium acetylicum gsk. the cloned chromosomal fragment contained an open reading frame which encodes a polypeptide of 308 amino acids with a molecular mass of 33349 da. the ex. aurantiacum gsk shows strong sequence similarity to b. acetylicum gsk, but possesses five additional amino acids at the c-terminus. an mr 392 ... | 1998 | 9804993 |
| clinical microbiology of coryneform bacteria. | coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ... | 1997 | 8993861 |
| characterisation of exiguobacterium aurantiacum isolates from blood cultures of six patients. | exiguobacterium spp. are alkaliphilic, halotolerant, non-spore-forming gram-positive bacilli, hitherto uncharacterized from human infections. six isolates of exiguobacterium aurantiacum were obtained from patients with bacteraemia, three of whom had myeloma. all isolates formed orange-yellow pigmented colonies on blood agar, were catalase- and dnase-positive, and grew on nutrient agar at ph 10 and in the presence of nacl 6% w/v. the six isolates were susceptible to all antimicrobial agents teste ... | 2007 | 17645563 |
| unique diversity of carotenoid-producing bacteria isolated from misasa, a radioactive site in japan. | we obtained carotenoid-producing microorganisms at a high frequency from water samples collected at misasa (tottori, japan), a region known for its high natural radioactivity content. a comprehensive 16s rrna gene-based phylogenetic analysis revealed that the 104 potential carotenoid producers isolated from misasa could be classified into 38 different species belonging to seven bacterial classes (flavobacteria, sphingobacteria, alpha-proteobacteria, gamma-proteobacteria, deinococci, actinobacter ... | 2007 | 17828533 |
| microbiological study of the body wall lesions of the echinoid tripneustes gratilla. | the microbiota of the body wall lesions of the echinoid tripneustes gratilla, initiated by the grazing action of the parasitic gastropod vexilla vexillum, was investigated with a pluridisciplinary approach. parasitised sea urchins showed body wall lesions strongly infected by bacteria that progressed through the test and reached the coelomic cavity after ca. 1 mo. we report here on the bacterial community observed in lesions of echinoids collected in situ and on the bacteria that successively ap ... | 2007 | 17933399 |
| effects of h2o2 under low- and high-aeration-level conditions on growth and catalase activity in exiguobacterium oxidotolerans t-2-2t. | the effects of h2o2 under low- and high-aeration-level conditions on growth and catalase activity in exiguobacterium oxidotolerans t-2-2t were investigated. continuous addition of 5-200 mm h2o2 to the culture medium from the mid-exponential growth phase enhanced the growth of the strain under the low-aeration-level condition, whereas the addition of 5-50 mm h2o2 decreased intracellular specific catalase activity and extracellular total catalases activity. the detection of extracellular catalase ... | 2007 | 18215632 |
| metabolic activity of siberian permafrost isolates, psychrobacter arcticus and exiguobacterium sibiricum, at low water activities. | the siberian permafrost is an extreme, yet stable environment due to its continuously frozen state. microbes maintain membrane potential and respiratory activity at average temperatures of -10 to -12 degrees c that concentrate solutes to an aw=0.90 (5 osm), the isolation of viable psychrobacter arcticus sp. 273-4 and exiguobacterium sibiricum sp. 255-15 from ancient permafrost suggests that these bacteria have maintained some level of metabolic activity for thousands of years. permafrost water a ... | 2008 | 18335164 |
| use of bromodeoxyuridine immunocapture to identify psychrotolerant phenanthrene-degrading bacteria in phenanthrene-enriched polluted baltic sea sediments. | the aim of this study was to enrich and identify psychrotolerant phenanthrene-degrading bacteria from polluted baltic sea sediments. polyaromatic hydrocarbon (pah)-contaminated sediments were spiked with phenanthrene and incubated for 2 months in the presence of bromodeoxyuridine that is incorporated into the dna of replicating cells. the bromodeoxyuridine-incorporated dna was extracted by immunocapture and analyzed by terminal-restriction fragment length polymorphism and 16s rrna gene cloning a ... | 2008 | 18616589 |
| bioremoval of hexavalent chromium from water by a salt tolerant bacterium, exiguobacterium sp. gs1. | pollution of terrestrial surfaces and aquatic systems by hexavalent chromium, cr(vi), is a worldwide public health problem. a chromium resistant bacterial isolate identified as exiguobacterium sp. gs1 by 16s rrna gene sequencing displayed high rate of removal of cr(vi) from water. exiguobacterium sp. gs1 is 99% identical to exiguobacterium acetylicum. the isolate significantly removed cr(vi) at both high and low concentrations (1-200 microg ml(-1)) within 12 h. the michaelis-menten k ( m ) and v ... | 2008 | 18663504 |
| chromate resistance, transport and bioreduction by exiguobacterium sp. zm-2 isolated from agricultural soil irrigated with tannery effluent. | bacterial strain exiguobacterium sp. zm-2 isolated from agricultural soil irrigated with tannery effluents, was examined for its resistance to hexavalent chromium. exiguobacterium sp. zm-2 could resist 12.37 mm of potassium chromate. the isolate was also found resistant to other heavy metal ions. exiguobacterium sp. zm-2 was able to reduce 500 microm hexavalent chromium completely within 56 h under in vitro conditions. chromate reduction was severely affected in presence of metabolic inhibitors, ... | 2008 | 18759228 |
| exiguobacterium soli sp. nov., a psychrophilic bacterium from the mcmurdo dry valleys, antarctica. | a novel psychrophilic bacterium, designated strain dvs 3y(t), was isolated from a moraine sample from the mcmurdo dry valleys, antarctica. phenotypic and chemotaxonomic characteristics and data from a phylogenetic analysis based on 16s rrna gene sequences indicated that strain dvs 3y(t) was related to the genus exiguobacterium. strain dvs 3y(t) exhibited >97 % gene sequence similarity with respect to exiguobacterium acetylicum dsm 20416(t) (97.4 %), exiguobacterium oxidotolerans dsm 17272(t) (97 ... | 2008 | 18842873 |
| architecture of thermal adaptation in an exiguobacterium sibiricum strain isolated from 3 million year old permafrost: a genome and transcriptome approach. | many microorganisms have a wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments, however not many have been fully explored over their entire growth temperature range through a holistic view of its physiology, genome, and transcriptome. we used exiguobacterium sibiricum strain 255-15, a psychrotrophic bacterium from 3 million year old siberian permafrost that grows from -5 degrees c to 39 degrees c to study its thermal adaptation. | 2008 | 19019206 |
| biogeography of two cold-adapted genera: psychrobacter and exiguobacterium. | the genera exiguobacterium and psychrobacter have been frequently detected in and isolated from polar permafrost and ice. these two genera have members that can grow at temperatures as low as -5 and -10 degrees c, respectively. we used quantitative pcr (q-pcr) to quantify members of these genera in 54 soil or sediment samples from polar, temperate and tropical environments to determine to what extent they are selected by cold environments. these results were further analyzed by multiple linear r ... | 2009 | 19322243 |
| molecular analysis of antioxidant genes in the extremohalophile marine bacterium exiguobacterium sp. cnu020. | exiguobacterium sp. cnu020, an alkaliphile and extremohalophile bacterium, is resistant to 20 mm h(2)o(2), a concentration that is lethal to most bacteria. representative antioxidant genes of catalase and superoxide dismutase (sod), obtained by pcr amplification of the genomic dna, were characterized: the 252-bp catalase gene shared 77% similarity in the deduced amino acid sequence to that of e. oxidotolerans t-2-2(t). the 420-bp sod gene had the closest similarity (94.3%) to the manganese-sod o ... | 2009 | 19404744 |
| effect of alkaline protease-producing exiguobacterium sp. ys1 inoculation on the solubilization and bacterial community of waste activated sludge. | a new approach to the solubilization of waste activated sludge (was) using an alkaline protease-producing bacterial isolate, exiguobacterium sp. ys1, was investigated under controlled mild alkaline conditions at ph 10. compared with the noninoculated experiment, the inoculated experiment in an anaerobic bioreactor increased soluble chemical oxygen demand concentration and alkaline protease activity by more than 40%, indicating a synergistic effect could be achieved when both bacterial inoculatio ... | 2009 | 19467863 |
| role of the charged amino acid residues in the cytoplasmic loop between putative transmembrane segments 6 and 7 of na+-atpase of an alkaliphilic bacterium, exiguobacterium aurantiacum. | atpase activity of the membrane-bound na(+)-atpase of an alkaliphilic bacterium, exiguobacterium aurantiacum, was measured in various concentrations of nacl. hill plot analysis showed a hill number of 1.7 with 5.2 mm as the k(0.5) value for na(+). when the site-directed mutagenesis of seven charged amino acid residues in the cytoplasmic loop (l6/7) between putative transmembrane segments 6 and 7 of the enzyme was conducted, all the mutated enzymes exhibited hill numbers close to that of the wild ... | 2009 | 19702882 |
| molecular and phylogenetic analysis of pyridoxal phosphate-dependent acyltransferase of exiguobacterium acetylicum. | the pyridoxal-5'-phosphate (plp)-dependent family of enzymes is a very diverse group of proteins that metabolize small molecules like amino acids and sugars, and synthesize cofactors for other metabolic pathways through transamination, decarboxylation, racemization, and substitution reactions. in this study we employed degenerated primer-based pcr amplification, using genomic dna isolated from the soil bacterium exiguobacterium acetylicum strain sn as template. we revealed the presence of a plp- ... | 2009 | 20158163 |
| decolorization of reactive dark blue k-r by the synergism of fungus and bacterium using response surface methodology. | the objective of this study was to investigate the potential ability of decolorizing reactive dark blue k-r by the synergism of fungal strain penicillium sp. qq and bacterial strain exiguobacterium sp. tl. the optimal conditions for decolorization determined by response surface methodology (rsm) were as follows: 12.5% (w/v) of both strains qq and tl, 0.19% (w/v) of glucose, 0.09% (w/v) of yeast extract, ph7.0, 37 degrees c and 200mg/l of initial dye concentration. it was shown that about 97% of ... | 2010 | 20566285 |
| a novel light-dependent selection marker system in plants. | photosensitizers are common in nature and play diverse roles as defense compounds and pathogenicity determinants and as important molecules in many biological processes. toxoflavin, a photosensitizer produced by burkholderia glumae, has been implicated as an essential virulence factor causing bacterial rice grain rot. toxoflavin produces superoxide and h₂o₂ during redox cycles under oxygen and light, and these reactive oxygen species cause phytotoxic effects. to utilize toxoflavin as a selection ... | 2011 | 20731786 |
| complete genome sequence of the thermophilic bacterium exiguobacterium sp. at1b. | here we present the genome of strain exiguobacterium sp. at1b, a thermophilic member of the genus exiguobacterium whose representatives were isolated from various environments along a thermal and physicochemical gradient. this genome was sequenced to be a comparative resource for the study of thermal adaptation with a psychroactive representative of the genus, exiguobacterium sibiricum strain 255-15, that was previously sequenced by the u.s. department of energy's (doe's) joint genome institute ... | 2011 | 21460088 |
| lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: comparison with detergent micelles, bicelles and liposomes. | production of integral membrane proteins (imps) in a folded state is a key prerequisite for their functional and structural studies. in cell-free (cf) expression systems membrane mimicking components could be added to the reaction mixture that promotes imp production in a soluble form. here lipid-protein nanodiscs (lpns) of different lipid compositions (dmpc, dmpg, popc, popc/dopg) have been compared with classical membrane mimicking media such as detergent micelles, lipid/detergent bicelles and ... | 2011 | 22056981 |
| exiguobacterium aquaticum sp. nov., a new member of the genus exiguobacterium. | a gram positive, motile, short rod shaped, orange pigmented bacterium strain imtb-3094t, isolated from water sample, collected from tikkar tal lake, haryana and was subjected to a detailed polyphasic taxonomic study. strain imtb-3094t, matched with most of the phenotypic and chemotaxonomic properties of the genus exiguobacterium and represented novel species. according to 16s rrna gene sequence analysis, strain imtb-3094t belonged to the genus exiguobacterium. the strain imtb-3094t exhibited hig ... | 2011 | 22058319 |
| characterization of acetonitrile-tolerant marine bacterium exiguobacterium sp. sbh81 and its tolerance mechanism. | a gram-positive marine bacterium, exiguobacterium sp. sbh81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. investigation of its tolerance mechanisms illustrated that it does not rely on solvent inactivation processes or modification of cell surface characteristics, but rather, increase of ... | 2011 | 21971080 |