| evidence for a close phylogenetic relationship between melissococcus pluton, the causative agent of european foulbrood disease, and the genus enterococcus. | the 16s rrna gene sequence of melissococcus pluton, the causative agent of european foulbrood disease, was determined in order to investigate the phylogenetic relationships between this organism and other low-g + c-content gram-positive bacteria. a comparative sequence analysis revealed that m. pluton is a close phylogenetic relative of the genus enterococcus. | 1994 | 8186103 |
| a pcr detection method for rapid identification of melissococcus pluton in honeybee larvae. | melissococcus pluton is the causative agent of european foulbrood, a disease of honeybee larvae. this bacterium is particularly difficult to isolate because of its stringent growth requirements and competition from other bacteria. pcr was used selectively to amplify specific rrna gene sequences of m. pluton from pure culture, from crude cell lysates, and directly from infected bee larvae. the pcr primers were designed from m. pluton 16s rrna sequence data. the pcr products were visualized by aga ... | 1998 | 9572987 |
| geographically diverse australian isolates of melissococcus pluton exhibit minimal genotypic diversity by restriction endonuclease analysis. | melissococcus pluton, the causative agent of european foulbrood is an economically significant disease of honey bees (apis mellifera) across most regions of the world and is prevalent throughout most states of australia. 49 isolates of m. pluton recovered from diseased colonies or honey samples in new south wales, queensland, south australia, tasmania and victoria were compared using sds-page, western immunoblotting and restriction endonuclease analyses. dna profiles of all 49 geographically div ... | 1999 | 10227161 |
| atopobacter phocae gen. nov., sp. nov., a novel bacterium isolated from common seals. | two strains of a gram-positive, catalase-negative, facultatively anaerobic, rod-shaped bacterium isolated from common seals were characterized using phenotypic and molecular taxonomic methods. the two strains closely resembled each other based on their biochemical characteristics, and page analysis of whole-cell protein patterns confirmed their close phenotypic affinity. 16s rrna gene sequencing showed that the two strains were genetically highly related (99.8% sequence similarity) and that they ... | 2000 | 11034483 |
| a nested array of rrna targeted probes for the detection and identification of enterococci by reverse hybridization. | complete 23s and almost complete 16s rrna gene sequences were determined for the type strains of the validly described enterococcus species, melissococcus pluton and tetragenococcus halophilus. a comprehensive set of rrna targeted specific oligonucleotide hybridization probes was designed according to the multiple probe concept. in silico probe design and evaluation was performed using the respective tools of the arb program package in combination with the arb databases comprising the currently ... | 2000 | 11249027 |
| preliminary investigations into possible resistance to oxytetracycline in melissococcus plutonius, a pathogen of honeybee larvae. | to investigate the occurrence of oxytetracycline (otc) resistance in melissococcus plutonius, which causes european foulbrood in honeybee colonies. | 2003 | 12485336 |
| tyramine functions as a toxin in honey bee larvae during varroa-transmitted infection by melissococcus pluton. | from wounds of honey bee pupae, caused by the mite varroa destructor, coccoid bacteria were isolated and identified as melissococcus pluton. the bacterial isolate was grown anaerobically in sorbitol medium to produce a toxic compound that was purified on xad columns, gelfiltration and preparative hplc. the toxic agent was identified by gc-ms and fticr-ms as tyramine. the toxicity of the isolated tyramine was tested by a novel mobility test using the protozoon stylonychia lemnae. a concentration ... | 2004 | 15109733 |
| distribution of melissococcus plutonius in honeybee colonies with and without symptoms of european foulbrood. | a sensitive hemi-nested polymerase chain reaction (pcr) was used for detection of melissococcus plutonius, the causative agent of european foulbrood (efb). sampling was made in switzerland, where efb is a widespread disease and incidences have increased in recent years. larvae from brood samples with and without clinical signs of disease (n=92) and honey (n=92) from the same colonies were investigated. individual larvae (n=60) and pupae (n=30) from diseased brood in single colonies were also inv ... | 2005 | 16254762 |
| catellicoccus marimammalium gen. nov., sp. nov., a novel gram-positive, catalase-negative, coccus-shaped bacterium from porpoise and grey seal. | two strains of an unknown gram-positive, catalase-negative, facultatively anaerobic, coccus-shaped bacterium, originating from a porpoise and a grey seal, were characterized using phenotypic, biochemical and molecular phylogenetic methods. chemical studies revealed the presence of a cell-wall murein based on l-lysine (type l-lys-gly-d-asp) and a dna g+c content of 38 mol%. comparative 16s rrna gene sequencing showed that this unidentified coccus-shaped organism formed a hitherto unknown subline ... | 2006 | 16449452 |
| hydrogen production by anaerobic microbial communities exposed to repeated heat treatments. | biological hydrogen production by anaerobic mixed communities was studied in laboratory-scale bioreactors using sucrose as the substrate. a bioreactor in which a fraction of the return sludge was exposed to repeated heat treatments performed better than a control bioreactor without repeated heat treatment of return sludge and produced a yield of 2.15 moles of hydrogen per mole of sucrose, with 50% hydrogen in the biogas. terminal restriction fragment length polymorphism analysis showed that two ... | 2007 | 17910366 |
| the occurrence of melissococcus plutonius in healthy colonies of apis mellifera and the efficacy of european foulbrood control measures. | european foulbrood (efb) persists in england and wales despite current treatment methods, all of which include feeding honey bee colonies with the antibiotic oxytetracycline (otc). a large-scale field experiment was conducted to monitor a husbandry-based method, using comb replacement (known as shook swarm), as a drug free efb control option. the understanding of efb epidemiology is limited, with little information on the presence of melissococcus plutonius in disease free colonies. additional s ... | 2010 | 20600088 |
| complete genome sequence of melissococcus plutonius atcc 35311. | we report the first completely annotated genome sequence of melissococcus plutonius atcc 35311. m. plutonius is a one genus one species bacterium, and the etiological agent of european foulbrood of the honey bee. the genome sequence will provide new insights into the molecular mechanisms underlying its pathogenicity. | 2011 | 21622755 |
| polar tube protein gene diversity among nosema ceranae strains derived from a greek honey bee health study. | honey bee samples from 54 apiaries originating from 37 geographic locations of greece were screened for nosema apis and nosema ceranae. furthermore 15 samples coming from 12 geographic locations were screened also for paenibacilluslarvae and melissococcus plutonius and seven honey bee virus species, for the first time on a nation-wide level. there was a tendency in finding proportionally higher spore counts in samples from apiaries that suffered important colony losses. p. larvae bacteria were i ... | 2011 | 21802424 |