| identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria. | a total of 84 of 150 vancomycin-resistant (defined as no inhibition of bacterial growth around a 30-micrograms vancomycin disk placed on 5% sheep blood-trypticase soy agar [bbl microbiology systems, cockeysville, md.]) bacteria were definitively identified by determining the phenotypic criteria. the identity of representatives was also confirmed by dna-dna hybridizations. the following strains were identified: 1 enterococcus faecium, 18 leuconostoc mesenteroides, 15 leuconostoc citreum, 9 leucon ... | 1989 | 2723037 |
| phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis. | a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ... | 2003 | 12514026 |
| biodiversity of lactic acid bacteria in moroccan soft white cheese (jben). | the bacterial diversity occurring in traditional moroccan soft white cheese, produced in eight different regions in morocco, was studied. a total of 164 lactic acid bacteria were isolated, purified and identified by whole-cell protein fingerprinting and rep-pcr genomic fingerprinting. the majority of the strains belonged to the genera lactobacillus, lactococcus, leuconostoc and enterococcus. sixteen species were identified: lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus paracase ... | 2005 | 16168579 |
| metabolic engineering of escherichia coli: construction of an efficient biocatalyst for d-mannitol formation in a whole-cell biotransformation. | a whole-cell biotransformation system for the conversion of d-fructose to d-mannitol was developed in escherichia coli by construction of a recombinant oxidation/reduction cycle. first, the mdh gene encoding for the mannitol dehydrogenase of leuconostoc pseudomesenteroides atcc 12291 (mdh) was expressed, effecting a strong catalytic activity of a nadh-dependent reduction of d-fructose to d-mannitol in cell extracts of the recombinant e. coli strain but not enabling whole cells of the strain to p ... | 2003 | 15296170 |
| diversity of lactic acid bacteria isolated from aoc salers cheese. | the objective of this work was to describe the diversity of lactic acid bacteria in traditional raw milk salers cheeses at the species and strain levels. the characterization of 381 strains isolated during ripening and various strain collections was investigated using physiological analysis and molecular techniques: rep-pcr, species and genus specific amplifications and the sequence analysis of 16s rdna for strain typing and taxonomic identification. the strains belonged to lactobacillus plantar ... | 2004 | 15190953 |
| metabolic engineering of escherichia coli: construction of an efficient biocatalyst for d-mannitol formation in a whole-cell biotransformation. | a whole-cell biotransformation system for the conversion of d-fructose to d-mannitol was developed in escherichia coli by constructing a recombinant oxidation/reduction cycle. first, the mdh gene, encoding mannitol dehydrogenase of leuconostoc pseudomesenteroides atcc 12291 (mdh), was expressed, effecting strong catalytic activity of an nadh-dependent reduction of d-fructose to d-mannitol in cell extracts of the recombinant e. coli strain. by contrast whole cells of the strain were unable to pro ... | 2004 | 14586579 |
| characterization of new bacteriocinogenic lactic acid bacteria isolated using a medium designed to simulate inhibition of listeria by lactobacillus sakei 2512 on meat. | bacteriocinogenic bacteria have been proposed to protect food products from listeria contamination as bioprotective cultures. lactobacillus sakei 2512 was demonstrated to inhibit the growth of listeria on sliced cooked ham by challenge test. a liquid medium simulating ham, bhi5l200, was designed in order to select bioprotective strains for meat protection. two strains were selected, from the 201 lactic acid bacteria screened, that produced bacteriocins at ph 5.8 in bhi5l200. the first one, leuco ... | 2007 | 16997410 |
| diversity of lactic acid bacteria in suan-tsai and fu-tsai, traditional fermented mustard products of taiwan. | fu-tsai and suan-tsai are spontaneously fermented mustard products traditionally prepared by the hakka tribe of taiwan. we chose 5 different processing stages of these products for analysis of the microbial community of lactic acid bacteria (lab) by 16s rrna gene sequencing. from 500 lab isolates we identified 119 representative strains belonging to 5 genera and 18 species, including enterococcus (1 species), lactobacillus (11 species), leuconostoc (3 species), pediococcus (1 species), and weiss ... | 2009 | 19700215 |
| fluorescence in situ hybridisation detection of lactobacillus plantarum group on olives to be used in natural fermentations. | at present there are very few studies on the bacterial diversity of olives and on the importance of the microbial species for the fermentation of olives aimed to table olives production. most of the authors report on the occurrence of lactobacillus plantarum as principal member of these communities or at least as the species responsible for the fermentation. in this study, fluorescence in situ hybridisation (fish) with 16s rrna probes was used to evaluate the occurrence of l. plantarum in olives ... | 2006 | 17052794 |
| expression of glf z.m. increases d-mannitol formation in whole cell biotransformation with resting cells of corynebacterium glutamicum. | a recombinant oxidation/reduction cycle for the conversion of d-fructose to d-mannitol was established in resting cells of corynebacterium glutamicum. whole cells were used as biocatalysts, supplied with 250 mm sodium formate and 500 mm d-fructose at ph 6.5. the mannitol dehydrogenase gene (mdh) from leuconostoc pseudomesenteroides was overexpressed in strain c. glutamicum atcc 13032. to ensure sufficient cofactor [nicotinamide adenine dinucleotide (reduced form, nadh)] supply, the fdh gene enco ... | 2007 | 17503033 |
| biodiversity of the microbial community in a spanish farmhouse cheese as revealed by culture-dependent and culture-independent methods. | the microbial diversity within alberquilla cheese, made from a spontaneously fermented mixture of raw goats' and sheep's milk in the alpujarra mountains (granada, south-east spain), has been studied by the classical culturing method and also by molecular analysis of community dna. a collection of 206 isolates was obtained from the cheese on different selective/differential media, which were then re-grouped to 52 after randomly amplified polymorphic dna (rapd)-pcr analyses. isolates on man-rogosa ... | 2008 | 18692931 |
| identification of lactic acid bacteria isolated from tarhana, a traditional turkish fermented food. | tarhana is a traditional fermented product produced from a mixture of spontaneously fermented yogurt and wheat flour in turkey. the aims of the present study were to enumerate and identify for the first time by molecular biology-based methods predominant lactic acid bacteria (lab) isolated during processing of tarhana. samples were collected from eight different regions of turkey. in order to explore the relationship between raw material and the microbiology of tarhana, yogurt and wheat flour we ... | 2009 | 19703719 |
| characterization of lactic acid bacteria from musts and wines of three consecutive vintages of ribeira sacra. | this study was designed to isolate and characterize the lactic acid microbiota of the musts and wines of a young denomination of origin area, ribeira sacra in north-west spain. | 2011 | 21204877 |
| improving d-mannitol productivity of escherichia coli: impact of nad, co2 and expression of a putative sugar permease from leuconostoc pseudomesenteroides. | the highly productive whole-cell biotransformation of d-fructose to d-mannitol with recombinant, resting cells of escherichia coli bl21(de3) requires the combined expression of mdh, fdh and glf which encode mannitol and formate dehydrogenases and a sugar facilitator, respectively. however, long-term stability of the system was restricted, possibly due to loss of the cofactor nad, high concentrations of formate, formation of co(2) affecting the internal ph of the cells, accumulation of high intra ... | 2009 | 19558963 |
| isolation and characteristics of lactic acid bacteria from koshu vineyards in japan. | to isolate, characterize and identify lactic acid bacteria (lab) in the vineyards where koshu grapes, a primary wine grape cultivar in japan, are grown. | 2008 | 18673430 |
| identification and characterization of lactic acid bacteria isolated from tibetan qula cheese. | fourteen strains of lactic acid bacteria (lab) isolated from qula, a tibetan traditional yak cheese, were divided into four groups (a-d) according to morphological and biochemical characteristics. on the basis of the 16s rrna gene sequence analysis, group a and group b strains were placed in the cluster making up the genus leuconostoc, which together with leuconostoc mesenteroides and leuconostoc pseudomesenteroides, formed a distinct cluster. the group c strain was clearly identified as enteroc ... | 2008 | 18323681 |
| natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16s ribosomal rna and reca gene analysis. | one hundred and fifty-six strains isolated from corn (zea mays l.), forage paddy rice (oryza sativa l.), sorghum (sorghum bicolor l.) and alfalfa (medicago sativa l.) silages prepared on dairy farms were screened, of which 110 isolates were considered to be lactic acid bacteria (lab) according to their gram-positive and catalase-negative characteristics and, mainly, the lactic acid metabolic products. these isolates were divided into eight groups (a-h) based on the following properties: morpholo ... | 2011 | 21282025 |
| comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in ivory coast and brazil. | to compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one ivorian farm) and box (two brazilian farms) fermentations were carried out. all fermentations were studied through a multiphasic approach. in general, the temperature inside the fermenting mass increased throughout all fermentations and reached end-values of 42-48°c. the main end-products of pulp carbohydrate catabolism were ethanol, lactic acid, acetic acid, and/or mann ... | 2011 | 21569940 |
| d-mannitol production by resting state whole cell biotrans-formation of d-fructose by heterologous mannitol and formate dehydrogenase gene expression in bacillus megaterium. | an in vivo system was developed for the biotransformation of d-fructose into d-mannitol by the expression of the gene mdh encoding mannitol dehydrogenase (mdh) from leuconostoc pseudomesenteroides atcc12291 in bacillus megaterium. the nadh reduction equivalents necessary for mdh activity were regenerated via the oxidation of formate to carbon dioxide by coexpression of the gene fdh encoding mycobacterium vaccae n10 formate dehydrogenase (fdh). high-level protein production of mdh in b. megateriu ... | 2007 | 17619232 |
| characterization of lactic acid bacteria isolated from a greek dry-fermented sausage in respect of their technological and probiotic properties. | a total of 147 lactic acid bacteria was isolated from two types of naturally fermented dry sausages at four different stages of the ripening process studied in order to select the most suitable strains according to their technological characteristics including probiotic properties and antimicrobial activity against food-borne pathogens. identification of the isolates revealed that 90% were lactobacilli, 4% enterococci, 3% pediococcus sp. and sporadic isolates of weissella viridescens, leuconosto ... | 2003 | 22063449 |
| dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in ghana. | the ghanaian cocoa bean heap fermentation process was studied through a multiphasic approach, encompassing both microbiological and metabolite target analyses. a culture-dependent (plating and incubation, followed by repetitive-sequence-based pcr analyses of picked-up colonies) and culture-independent (denaturing gradient gel electrophoresis [dgge] of 16s rrna gene amplicons, pcr-dgge) approach revealed a limited biodiversity and targeted population dynamics of both lactic acid bacteria (lab) an ... | 2007 | 17277227 |
| molecular and technological characterization of lactic acid bacteria from traditional fermented sausages of basilicata region (southern italy). | lactic acid bacteria (lab) from traditional fermented sausages of the basilicata region were investigated by ardra-pcr and rapd-pcr for taxonomic identification at species and strain level and characterized on the basis of the growth and acidification at different temperatures, incubation times, levels of nacl and kno(2), hydrolysis of sarcoplasmatic and myofibrillar proteins and antimicrobial, peptide/amino acid release and nitrate reductase activities. lactobacillus sakei was the predominant s ... | 2008 | 22063864 |
| the microbiology of ghanaian cocoa fermentations analysed using culture-dependent and culture-independent methods. | export of cocoa beans is of great economic importance in ghana and several other tropical countries. raw cocoa has an astringent unpleasant taste and a spontaneous fermentation is the first step in a process leading to cocoa beans with the characteristic cocoa flavour and taste. the microbiology of ghanaian cocoa fermentations was investigated using culture-dependent and culture-independent methods. samples were collected at 12 hour intervals during 96-144 hour tray and traditional heap fermenta ... | 2007 | 17161485 |
| diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of gari, a traditional african food. | traditional fermentation of cassava is dominated by a lactic acid bacteria (lab) population. fermentation is important for improving product flavour and aroma as well as safety, especially by reduction of its toxic cyanogenic glucosides. the production of gari from cassava in benin typically occurs on a household or small industrial scale, and consequently suffers from inconsistent product quality and may not always be safe for consumption. therefore, the diversity of lab from a typical cassava ... | 2005 | 16104351 |
| improved mannitol production by a random mutant of leuconostoc pseudomesenteroides. | a mutant of leuconostoc pseudomesenteroides atcc12291 that was unable to grow on fructose was constructed by chemical mutagenesis. the fructose uptake of this mutant, designated as bpt143, was unaltered and allowed fructose still to be converted into mannitol when glucose was present in the growth medium. the mutant grew and consumed fructose faster than the parent strain when grown in a medium containing both glucose and fructose. the specific activity of fructokinase, the enzyme involved in ph ... | 2005 | 15707689 |
| lactobacillus reuteri atcc 53608 mdh gene cloning and recombinant mannitol dehydrogenase characterization. | a gene encoding mannitol-2-dehydrogenase (e.c. 1.1.1.138) (mdh) was cloned from lactobacillus reuteri and expressed in escherichia coli. the 1,008-bp gene encodes a protein consisting of 336 amino acids, with a predicted molecular mass of 35,920 da. the deduced amino acid sequence of l. reuteri mdh (lrmdh) is 77% and 76% similar to the mdhs from leuconostoc mesenteroides and leuconostoc pseudomesenteroides, respectively. the purified recombinant enzyme appears as a single band of 40 kda in sodiu ... | 2005 | 15630578 |
| plasmid-encoded diacetyl (acetoin) reductase in leuconostoc pseudomesenteroides. | a plasmid-borne diacetyl (acetoin) reductase (buta) from leuconostoc pseudomesenteroides chcc2114 was sequenced and cloned. nucleotide sequence analysis revealed an open reading frame encoding a protein of 257 amino acids which had high identity at the amino acid level to diacetyl (acetoin) reductases reported previously. downstream of the buta gene of l. pseudomesenteroides, but coding in the opposite orientation, a putative dna recombinase was identified. a two-step pcr approach was used to co ... | 2003 | 12514009 |
| characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis. | the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ... | 2004 | 15183874 |
| spontaneous formation of a mannitol-producing variant of leuconostoc pseudomesenteroides grown in the presence of fructose. | we report the spontaneous formation of a stable mannitol-producing variant of leuconostoc pseudomesenteroides. the mannitol-producing variant showed mannitol dehydrogenase activity which was absent in the parental strain. it was also able to use fructose and glucose simultaneously, whereas the parental strain showed diauxic growth with these sugars. a possible explanation of these observations is discussed. | 2001 | 11375210 |
| leuconostoc pseudomesenteroides as a cause of nosocomial urinary tract infections. | the phenotypic and genotypic characterization of five clinical isolates of leuconostoc pseudomesenteroides associated with nosocomially acquired urinary tract infections is described. all the strains were susceptible to chloramphenicol, clindamycin, erythromycin, gentamicin, and tetracycline; all were resistant to nalidixic acid, norfloxacin, and vancomycin; and all were intermediately affected by ampicillin and penicillin. analysis of chromosomal dna by pulsed-field gel electrophoresis after tr ... | 1999 | 10565942 |
| bacterial community dynamics during production of registered designation of origin salers cheese as evaluated by 16s rrna gene single-strand conformation polymorphism analysis. | microbial dynamics during processing and ripening of traditional cheeses such as registered designation of origin salers cheese, an artisanal cheese produced in france, play an important role in the elaboration of sensory qualities. the aim of the present study was to obtain a picture of the dynamics of the microbial ecosystem of rdo salers cheese by using culture-independent methods. this included dna extraction, pcr, and single-strand conformation polymorphism (sscp) analysis. bacterial and hi ... | 2003 | 12839752 |
| influence of lactobacillus spp. from an inoculant and of weissella and leuconostoc spp. from forage crops on silage fermentation | lactobacillus spp. from an inoculant and weissella and leuconostoc spp. from forage crops were characterized, and their influence on silage fermentation was studied. forty-two lactic acid-producing cocci were obtained from forage crops and grasses. all isolates were gram-positive, catalase-negative cocci that produced gas from glucose, and produced more than 90% of their lactate in the d-isomer form. these isolates were divided into groups a and b by sugar fermentation patterns. two representati ... | 1998 | 9687461 |
| the mannitol dehydrogenase gene (mdh) from leuconostoc mesenteroides is distinct from other known bacterial mdh genes. | the n-terminal amino acid sequences of the intact protein and three tryptic peptides from a 41 kda protein purified from a commercial mannitol dehydrogenase (mdh) enzyme preparation of leuconostoc mesenteroides atcc-9135 were determined. oligonucleotides deduced from these peptide sequences were used to isolate the putative mdh gene from l. mesenteroides using the vectorette system. nucleotide sequence analysis revealed an open reading frame (orf1) of 1,014 bp encoding a putative mdh protein of ... | 2002 | 12226722 |
| a zinc-containing mannitol-2-dehydrogenase from leuconostoc pseudomesenteroides atcc 12291: purification of the enzyme and cloning of the gene. | mannitol-2-dehydrogenase (ec 1.1.1.67) of leuconostoc pseudomesenteroides atcc 12291 catalyzing the nadh-dependent reduction of d-fructose to d-mannitol was purified to homogeneity. native mannitol-2-dehydrogenase has a molecular mass of 155 kda as determined by gel filtration chromatography. in sds-page, a single band appeared corresponding to a molecular mass of 43 kda which indicated that the enzyme was composed of four identical subunits. enzyme activity was completely inhibited by edta and ... | 2003 | 12560988 |
| [leuconostoc pseudomesenteroides bacteremia]. | | 1999 | 10323105 |
| differentiation of dextran-producing leuconostoc strains from fermented rice cake (puto) using pulsed-field gel electrophoresis. | lactic acid bacteria were isolated from puto, a fermented rice cake consumed as a breakfast and snack food in the philippines. the microflora was dominated by dextran-producing leuconostocs, and these were differentiated into four groups using pulsed-field gel electrophoresis of restriction enzyme digested chromosomal dna, in conjunction with taxonomic tests. the four groups corresponded to the species leuconostoc mesenteroides subsp. mesenteroides, leuconostoc pseudomesenteroides, leuconostoc c ... | 1995 | 7488529 |
| selection, application and monitoring of lactobacillus paracasei strains as adjunct cultures in the production of gouda-type cheeses. | raw milk cheeses have more intense flavours than cheeses made from pasteurized milk and harbour strains with potential adjunct properties. two lactobacillus paracasei strains, r-40926 and r-40937, were selected as potential adjunct cultures from a total of 734 isolates from good quality artisan raw milk gouda-type cheeses on the basis of their prevalence in different cheese types and/or over several production batches, safety and technological parameters. conventional culturing, isolation and id ... | 2010 | 21036412 |
| complete genome sequence of φmh1, a leuconostoc temperate phage. | bacteriophage φmh1, a newly isolated temperate phage from a uv-induced lysate of leuconostoc pseudomesenteroides, has an isometric head, a noncontractile tail, and a double-stranded dna genome with a length of 38709 bp. bioinformatic analysis of the phage genome revealed 65 putative open reading frames (orfs). predicted protein products of the orfs were determined and described. φmh1 can be classified as a member of the family siphoviridae by morphology and genome structure. the phage did not sh ... | 2010 | 20848296 |
| diversity of lactobacillus and bifidobacterium in feces of herbivores, omnivores and carnivores. | the lactobacillus and bifidobacterium population in the feces of 26 animals (16 species) were studied by culture-dependent and culture-independent techniques. lactobacilli were detected from a few herbivores, all carnivores and some omnivores. lactobacillus johnsonii, lactobacillus reuteri, lactobacillus salivarius, lactobacillus vaginalis and lactobacillus ingluviei were the most dominant lactobacilli in carnivores. these species were, however, not predominant in herbivores and omnivores. lacto ... | 2010 | 21034840 |
| lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration. | we investigated the effects of the predominant lactic acid bacteria (lab) on the fermentation characteristics and aerobic stability of total mixed ration (tmr) silage containing soybean curd residue (sc-tmr silage). the sc-tmr materials were ensiled in laboratory silos for 14 or 56 days. lab predominant in sc-tmr silage were identified (exp. 1). lactobacillus fermentum (l. fermentum) and streptococcus bovis (s. bovis) were found in the untreated materials, leuconostoc pseudomesenteroides (l. pse ... | 2016 | 26949952 |
| enzymatic activity of lactic acid bacteria (with antimicrobial properties) isolated from a traditional spanish cheese. | twenty-four strains of lactic acid bacteria (lab) isolated from a traditional spanish cheese (genestoso cheese) were evaluated for their enzymatic activities (acidifying and proteolytic abilities and carboxypeptidase, aminopeptidase, dipeptidase, caseinolytic and esterase activities), in order to select indigenous strains of technical interest for the manufacture of cheese. these strains were selected on the basis of their antimicrobial activity relative to five reference strains and were identi ... | 2010 | 20510776 |
| leuconostoc pseudomesenteroides blood stream infection following liver transplantation. | leuconostoc spp. are vancomycin resistant gram positive lactobacilli. little is known about their significance in transplant recipients. | 2010 | 20305321 |
| identification and characterization of novel multiple bacteriocins produced by leuconostoc pseudomesenteroides qu 15. | to characterize novel multiple bacteriocins produced by leuconostoc pseudomesenteroides qu 15. | 2010 | 20070442 |
| 16s ribosomal dna analysis and characterization of lactic acid bacteria associated with traditional tibetan qula cheese made from yak milk. | twenty strains of lactic acid bacteria were isolated from six traditional tibetan qula cheese made from yak which were collected from northwest china, including tibet, qinghai and gansu province. these isolates were subjected to phenotypic and genetic analyses. all isolates were gram-positive and catalase-negative cocci that produced gas from glucose and formed d(-) isomer of lactate. most isolates were able to grow in de man, rogosa and sharpe (mrs) broth at ph values 3.0-9.0 and in 6.5% nacl ( ... | 2010 | 21108692 |
| fermented soymilk increases voluntary wheel running activity and sexual behavior in male rats. | wheel running by rodents is thought to reflect voluntary exercise in humans. the present study examined the effect of fermented soymilk (fsm) on voluntary wheel running in rats. fsm was prepared from soymilk (sm) using the bacteria leuconostoc pseudomesenteroides. the rats were fed a normal diet for 3 weeks followed by a 3-week administration of diet containing fsm or sm (5% w/w), and then the diets were switched back to a normal diet for 3 weeks. the voluntary wheel running activity was increas ... | 2010 | 21164545 |
| genome sequence of leuconostoc pseudomesenteroides kctc 3652. | we announce the genome sequence of one of the most prevalent lactic acid bacteria present during the manufacturing process of cane juice, the type strain leuconostoc pseudomesenteroides kctc 3652 (3,244,985 bp, with a g+c content of 38.3%), which consists of 1,160 large contigs (>100 bp in size). all of the contigs were assembled by the newbler assembler 2.3 software program (454 life sciences). | 2011 | 21705609 |
| culture-independent detection of microorganisms in traditional slovakian bryndza cheese. | in order to investigate the microflora of slovakian bryndza cheese (a cheese containing unpasteurized or pasteurized ewes' milk component) by a culture-independent method, dna was extracted directly from 7 bryndza samples and analysed by an innovative method. using the universal prokaryotic and fungal primers, ribosomal dna internal transcribed spacer (its) regions with variable length were amplified. the standard universal reverse primer l1 aligning to bacterial 23s rdna was found unsuitable fo ... | 2011 | 21849217 |
| Microbial dynamics during the ripening of a mixed cow and goat milk cheese manufactured using frozen goat milk curd. | To overcome the seasonal shortage of goat milk in mixed milk cheese manufacture, pasteurized goat milk curd and high-pressure-treated raw goat milk curd manufactured in the spring were held at -24 °C for 4 mo, thawed, and mixed with fresh cow milk curd for the manufacture of experimental cheeses. Control cheeses were made from a mixture of pasteurized cow and goat milk. The microbiota of experimental and control cheeses was studied using culture-dependent and culture-independent techniques. Bact ... | 2011 | 21943728 |
| species diversity, community dynamics, and metabolite kinetics of the microbiota associated with traditional ecuadorian spontaneous cocoa bean fermentations. | traditional fermentations of the local ecuadorian cocoa type nacional, with its fine flavor, are carried out in boxes and on platforms for a short time. a multiphasic approach, encompassing culture-dependent and -independent microbiological analyses of fermenting cocoa pulp-bean samples, metabolite target analyses of both cocoa pulp and beans, and sensory analysis of chocolates produced from the respective fermented dry beans, was applied for the investigation of the influence of these fermentat ... | 2011 | 21926224 |
| identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage. | in order to understand the relationship between lactic acid bacteria (lab) species and silage fermentation, a total of 65 lab strains isolated from mixed pasture of timothy (phleum pratense l.) and orchardgrass (dactylis glomerata l.), and its badly preserved silages were subjected to phenotypic and genetic analysis. according to these analyses, the isolates were divided into 13 groups, including enterococcus gallinarum, lactobacillus acidipiscis, l. coryniformis subsp. coryniformis, l. corynifo ... | 2011 | 22515692 |
| hanseniaspora opuntiae, saccharomyces cerevisiae, lactobacillus fermentum, and acetobacter pasteurianus predominate during well-performed malaysian cocoa bean box fermentations, underlining the importance of these microbial species for a successful cocoa bean fermentation process. | two spontaneous malaysian cocoa bean box fermentations (one farm, two plantation plots) were investigated. physical parameters, microbial community dynamics, yeast and bacterial species diversity [mainly lactic acid bacteria (lab) and acetic acid bacteria (aab)], and metabolite kinetics were monitored, and chocolates were produced from the respective fermented dry cocoa beans. similar microbial growth and metabolite profiles were obtained for the two fermentations. low concentrations of citric a ... | 2013 | 23664257 |
| characterization of lactic acid bacteria from naturally-fermented manzanilla aloreña green table olives. | manzanilla aloreña (or aloreña) table olives are naturally fermented traditional green olives with a denomination of protection (dop). the aim of this study was to search for lactic acid bacteria (lab) with technological properties of interest for possible inclusion in a starter or protective culture preparation or also as probiotics. a collection of 144 lab obtained from aloreña green table olives naturally-fermented by four small-medium enterprises (smes) from málaga (spain), including lactoba ... | 2012 | 22986194 |
| application of protective cultures against listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere. | to control the growth, or reduce the numbers, of food pathogens such as listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere (map), the effectiveness of protective cultures was evaluated in this study. leuconostoc pseudomesenteroides pck18 reduced the counts of l. monocytogenes by 1.22 log cfu/g in chicken burgers under map after 24 d. furthermore, a reduction of 1.16 log cfu/g in c. jejuni together with a delay in the growth of lactic acid bacte ... | 2013 | 23472035 |
| evaluation of lactic acid bacteria and yeast diversity in traditional white pickled and fresh soft cheeses from the mountain regions of serbia and lowland regions of croatia. | the goal of this study was the characterisation of indigenous lactic acid bacteria (lab) and yeasts isolated from nine white pickled (bg) and nine fresh soft (zg) artisanal cheeses collected in serbia and croatia. while lab were present in all of the cheeses collected, yeasts were found in all bg cheeses but only in three zg cheese samples. high lab and yeast species diversity was determined (average h'(l)=0.4 and h'(y)=0.8, respectively). the predominant lab species in white pickled (bg) cheese ... | 2013 | 23973841 |
| behaviour of non-stressed and stressed listeria monocytogenes and campylobacter jejuni cells on fresh chicken burger meat packaged under modified atmosphere and inoculated with protective culture. | numerous investigations have provided evidence that chicken products are a source of listeria monocytogenes and campylobacter jejuni. different strategies applied in final products are needed to prevent consumers' contamination. in this work, the combination of modified atmosphere packaging (map) and protective culture to control the growth of freeze stressed and non-stressed l. monocytogenes and c. jejuni on fresh chicken meat burger was studied. meat burgers were inoculated with l. monocytogen ... | 2012 | 22831819 |
| metagenomics analysis of microbial communities associated with a traditional rice wine starter culture (xaj-pitha) of assam, india. | this is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving illumine-based whole genome shotgun (wgs) sequencing method. metagenomic dna was extracted from rice wine starter culture concocted by ahom community of assam and analyzed using a miseq(®) system. a total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. data obtained from the use of several taxonomic profiling tools were ... | 2016 | 28330225 |
| genome sequences of two leuconostoc pseudomesenteroides strains isolated from danish dairy starter cultures. | the lactic acid bacterium leuconostoc pseudomesenteroides can be found in mesophilic cheese starters, where it produces aromatic compounds from, e.g., citrate. here, we present the draft genome sequences of two l. pseudomesenteroides strains isolated from traditional danish cheese starters. | 2014 | 24903866 |
| identification of the receptor-binding protein in lytic leuconostoc pseudomesenteroides bacteriophages. | two phages, p793 and φln04, sharing 80.1% nucleotide sequence identity but having different strains of leuconostoc pseudomesenteroides as hosts, were selected for identification of the host determinant gene. construction of chimeric phages leading to the expected switch in host range identified the host determinant genes as orf21p793/orf23φln04. the genes are located in the tail structural module and have low sequence similarity at the distal end. | 2013 | 23503306 |
| characterisation of the spoilage bacterial microbiota in oyster gills during storage at different temperatures. | the spoilage bacterial community in oyster gill was investigated during storage at 4, 10 and 20 °c. aerobic plate counts and ph values were determined. total bacterial dna was extracted from oyster gill and bulk cells of plate count media. the major bacterial species during fresh or different temperatures storage were determined by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). | 2013 | 23696433 |
| characterization of leucocin b-km432bz from leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin pa-1. | a bacteriocin-producing bacterium was isolated from boza and identified as leuconostoc pseudomesenteroides km432bz. the antimicrobial peptide was purified and shown to be identical to other class iia bacteriocins: leucocin a from leuconostoc gelidum ual-187 and leuconostoc pseudomesenteroides qu15 and leucocin b from leuconostoc carnosum ta11a. the bacteriocin was named leucocin b-km432bz. leucocin b-km432bz gene cluster encodes the bacteriocin precursor (lcnb), the immunity protein (lcni) and t ... | 2013 | 23936441 |
| classification of lytic bacteriophages attacking dairy leuconostoc starter strains. | a set of 83 lytic dairy bacteriophages (phages) infecting flavor-producing mesophilic starter strains of the leuconostoc genus was characterized, and the first in-depth taxonomic scheme was established for this phage group. phages were obtained from different sources, i.e., from dairy samples originating from 11 german dairies (50 leuconostoc pseudomesenteroides [ln. pseudomesenteroides] phages, 4 ln. mesenteroides phages) and from 3 external phage collections (17 ln. pseudomesenteroides phages, ... | 2013 | 23563949 |
| characteristics of microbial biofilm on wooden vats ('gerles') in pdo salers cheese. | the purpose of this study was to characterize microbial biofilms from 'gerles' (wooden vats for making pdo salers cheese) and identify their role in milk inoculation and in preventing pathogen development. gerles from ten farms producing pdo salers cheese were subjected to microbial analysis during at least 4 periods spread over two years. they were distinguished by their levels of lactobacillus (between 4.50 and 6.01 log cfu/cm(2)), gram negative bacteria (between 1.45 and 4.56 log cfu/cm(2)), ... | 2012 | 22483401 |
| characterization of lactic acid bacteria isolated from artisanal travnik young cheeses, sweet creams and sweet kajmaks over four seasons. | the aim of this study was to investigate the composition of lactic acid bacteria (lab) in autochthonous young cheeses, sweet creams and sweet kajmaks produced in the vlašić mountain region of central bosnia and herzegovina near the town of travnik over a four season period. these three products were made from cow's milk by a traditional method without the addition of a starter culture. preliminary characterization with phenotype-based assays and identification using rep-pcr with a (gtg)5 primer ... | 2014 | 24387849 |
| [production of d-mannitol by metabolically engineered escherichia coli]. | d-mannitol has wide applications in food, pharmaceutical, and chemical industries. in this study, we constructed a genetically stable escherichia coli strain for d-mannitol production by integrating mannitol dehydrogenase (mdh) and fructose permease (fupl) genes of leuconostoc pseudomesenteroides atcc 12291 into chromosome of e. coli atcc 8739 and inactivating other fermentation pathways (including pyruvate formate-lyase, lactate dehydrogenase, fumarate reductase, alcohol dehydrogenase, methylgl ... | 2013 | 24432660 |
| natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant. | natural lactic acid bacteria (lab) populations in tropical grasses and their fermentation characteristics on silage prepared with cellulase enzyme and lab inoculants were studied. a commercial inoculant lactobacillus plantarum chikuso 1 (ch), a local selected strain lactobacillus casei th14 (th14), and 2 cellulases, acremonium cellulase (ac) and maicelase (mc; meiji seika pharma co. ltd., tokyo, japan), were used as additives to silage preparation with fresh and wilted (6 h) guinea grass and nap ... | 2016 | 27743669 |
| phenotypic and phylogenetic analysis of lactic acid bacteria isolated from forage crops and grasses in the tibetan plateau. | a total of 140 lactic acid bacteria (lab) strains were isolated from corn, alfalfa, clover, sainfoin, and indian goosegrass in the tibetan plateau. according to phenotypic and chemotaxonomic characteristics, 16s rdna sequence, and reca gene pcr amplification, these lab isolates were identified as belonging to five genera and nine species. corn contained more lab species than other forage crops. leuconostoc pseudomesenteroides, lactococcus lactis subsp. lactis, lactobacillus brevis, and weissella ... | 2012 | 22367939 |
| genotypic characterization and safety assessment of lactic acid bacteria from indigenous african fermented food products. | indigenous fermented food products play an essential role in the diet of millions of africans. lactic acid bacteria (lab) are among the predominant microbial species in african indigenous fermented food products and are used for different applications in the food and biotechnology industries. numerous studies have described antimicrobial susceptibility profiles of lab from different parts of the world. however, there is limited information on antimicrobial resistance profiles of lab from africa. ... | 2012 | 22594449 |
| biocide tolerance, phenotypic and molecular response of lactic acid bacteria isolated from naturally-fermented aloreña table to different physico-chemical stresses. | lactic acid bacteria (lab) isolated throughout the fermentation process of aloreña table olives were found to be resistant at least to three antibiotics (casado muñoz et al., 2014); however, most were sensitive to the biocides tested in this study (with minimum inhibitory concentrations [mic] below the epidemiological cut-off values). 2-15% of the isolates were found to be biocide resistant: leuconostoc pseudomesenteroides, which were resistant to hexachlorophene, and lactobacillus pentosus to c ... | 2016 | 27554140 |
| effects of exogenous yeast and bacteria on the microbial population dynamics and outcomes of olive fermentations. | in this study, we examined sicilian-style green olive fermentations upon the addition of saccharomyces cerevisiae ucdfst 09-448 and/or pichia kudriazevii ucdfst09-427 or the lactic acid bacteria (lab) lactobacillus plantarum aj11r and leuconostoc pseudomesenteroides bgm3r. olives containing s. cerevisiae ucdfst 09-448, a strain able to hydrolyze pectin, but not p. kudriazevii ucdfst 09-427, a nonpectinolytic strain, exhibited excessive tissue damage within 4 weeks. dna sequencing of fungal inter ... | 2017 | 28124026 |
| natural lactic acid bacteria population and silage fermentation of whole-crop wheat. | winter wheat is a suitable crop to be ensiled for animal feed and china has the largest planting area of this crop in the world. during the ensiling process, lactic acid bacteria (lab) play the most important role in the fermentation. we investigated the natural population of lab in whole-crop wheat (wcw) and examined the quality of whole-crop wheat silage (wcws) with and without lab inoculants. two lactobacillus plantarum subsp. plantarum strains, zhengzhou university 1 (zzu 1) selected from co ... | 2015 | 26104520 |
| diversity and technological potential of lactic acid bacteria of wheat flours. | lactic acid bacteria (lab) were analysed from wheat flours used in traditional bread making throughout sicily (southern italy). plate counts, carried out in three different media commonly used to detect food and sourdough lab, revealed a maximal lab concentration of approximately 4.75 log cfu g(-1). colonies representing various morphological appearances were isolated and differentiated based on phenotypic characteristics and genetic analysis by randomly amplified polymorphic dna (rapd)-pcr. fif ... | 2013 | 24010616 |
| antibiotic resistance of lactobacillus pentosus and leuconostoc pseudomesenteroides isolated from naturally-fermented aloreña table olives throughout fermentation process. | antimicrobial resistance of lactobacillus pentosus (n=59) and leuconostoc pseudomesenteroides (n=13) isolated from aloreña green table olives (which are naturally-fermented olives from málaga, spain) to 15 antibiotics was evaluated. most lb. pentosus (95%) and all lc. pseudomesenteroides were resistant to at least three antibiotics. principal component analysis determined that the prevalence of antibiotic resistance in lab throughout the fermentation process was highly dependent on the fermenter ... | 2014 | 24370969 |
| genomic characterization of dairy associated leuconostoc species and diversity of leuconostocs in undefined mixed mesophilic starter cultures. | undefined mesophilic mixed (dl-type) starter cultures are composed of predominantly lactococcus lactis subspecies and 1-10% leuconostoc spp. the composition of the leuconostoc population in the starter culture ultimately affects the characteristics and the quality of the final product. the scientific basis for the taxonomy of dairy relevant leuconostocs can be traced back 50 years, and no documentation on the genomic diversity of leuconostocs in starter cultures exists. we present data on the le ... | 2017 | 28217118 |
| whey powders are a rich source and excellent storage matrix for dairy bacteriophages. | thirteen whey powders and 5 whey powder formulations were screened for the presence of dairy bacteriophages using a representative set of 8 acid-producing lactococcus lactis and 5 streptococcus thermophilus, and 8 flavour-producing leuconostoc pseudomesenteroides and leuconostoc mesenteroides strains. lytic l. lactis phages were detected in all samples, while s. thermophilus and leuconostoc phages were present in 50% or 40% of the samples, respectively. maximal phage titers were 6×10(7) plaque-f ... | 2017 | 27835774 |
| pre-treatment step with leuconostoc mesenteroides or l. pseudomesenteroides strains removes furfural from zymomonas mobilis ethanolic fermentation broth. | furfural is an inhibitor of growth and ethanol production by zymomonas mobilis. this study used a naturally occurring (not gmo) biological pre-treatment to reduce that amount of furfural in a model fermentation broth. pre-treatment involved inoculating and incubating the fermentation broth with strains of leuconostoc mesenteroides or leuconostoc pseudomesenteroides. the leuconostoc strains converted furfural to furfuryl alcohol without consuming large amounts of dextrose in the process. coupling ... | 2014 | 25048957 |
| sequence and comparative analysis of leuconostoc dairy bacteriophages. | bacteriophages attacking leuconostoc species may significantly influence the quality of the final product. there is however limited knowledge of this group of phages in the literature. we have determined the complete genome sequences of nine leuconostoc bacteriophages virulent to either leuconostoc mesenteroides or leuconostoc pseudomesenteroides strains. the phages have dsdna genomes with sizes ranging from 25.7 to 28.4 kb. comparative genomics analysis helped classify the 9 phages into two cla ... | 2014 | 24561391 |
| in situ synthesis of exopolysaccharides by leuconostoc spp. and weissella spp. and their rheological impacts in fava bean flour. | fava bean flour is regarded as a potential plant-based protein source, but the addition of it at high concentration is restricted by its poor texture-improving ability and by anti-nutritional factors (anf). exopolysaccharides (eps) produced by lactic acid bacteria (lab) are regarded as good texture modifiers. in this study, fava bean flour was fermented with leuconostoc spp. and weissella spp. with or without sucrose addition, in order to evaluate their potential in eps production. the contents ... | 2017 | 28258980 |
| bacteremia due to leuconostoc pseudomesenteroides in a patient with acute lymphoblastic leukemia: case report and review of the literature. | leuconostoc species are vancomycin-resistant gram-positive cocci. infections due to leuconostoc species have been reported in various immunocompromised patients, but little is known about such infection in patients with hematologic malignancies. we report a case of leuconostoc infection in a 44-year-old woman with acute lymphoblastic leukemia. the patient developed a high fever despite antimicrobial therapy with doripenem after induction chemotherapy. after an isolate from blood cultures was ide ... | 2016 | 27635268 |
| antibiotic lock therapy for leuconostoc pseudomesenteroides catheter-related bacteremia. | | 2016 | 27524227 |
| russian kefir grains microbial composition and its changes during production process. | by combining dgge-pcr method, classical microbiological analysis and light- and electron microscopic observations, it was found that the composition of microbial communities of central russia regions kefir grains, starter and kefir drink include bacteria of the genera lactobacillus, leuconostoc and lactococcus, and yeast anamorphs of the genera saccharomyces, kazachstania and gibellulopsis. fifteen prokaryotic and four eukaryotic pure cultures of microorganisms were isolated and identified from ... | 2016 | 27539304 |
| identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves. | from 6 samples of tropically grown fruits and leaves, 10 lactic acid bacteria belonging leuconostoc, weissella, and lactobacillus species were isolated and identified by 16s rrna gene sequencing and (gtg)5 fingerprinting. acidification kinetics determined from bhi broth cultures showed genus-related patterns. in particular, weissella cibaria appeared to act as a potent acidifier. tolerance of isolates to acid, oxidative, or salt stress was highly variable and strain dependent. isolate s14 (leuco ... | 2016 | 27197991 |
| a mixed infection of leuconostoc lactis and vancomycin-resistant enterococcus in a liver transplant recipient. | bacterial infection in patients who have undergone liver transplantation is a major complication of the procedure. leuconostoc spp. are important pathogenic bacteria in individuals with poor immune function, especially transplant patients. in this report, we describe the case of a 45-year-old asian male liver transplant recipient who was initially preliminarily diagnosed with infection with leuconostoc pseudomesenteroides by using the microbial tests of the vitek 2 system and the aesculin hydrol ... | 2012 | 22878250 |
| genome sequence of leuconostoc pseudomesenteroides strain 4882, isolated from a dairy starter culture. | the nonstarter lactic acid bacterium leuconostoc pseudomesenteroides is a species widely found in the dairy industry and plays a key role in the formation of aromatic compounds. here, we report the first genome sequence of a dairy strain of leuconostoc pseudomesenteroides, which is 2 mb. | 2012 | 23144391 |
| production, extraction and characterization of exopolysaccharides produced by the native leuconostoc pseudomesenteroides r2 strain. | the genus leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (eps). these biopolymers possess considerable commercial potential. because of the wide variety of industrial applications of eps, this study aimed to produce and characterize the native exopolysaccharide strain leuconostoc pseudomesenteroides r2, which was isolated from cabbage collected in a semi-arid region of bahi ... | 2012 | 22652760 |
| control of escherichia coli and listeria monocytogenes in suckling-lamb meat evaluated using microbial challenge tests. | escherichia coli and listeria monocytogenes microbial challenge tests were performed on fresh suckling-lamb meat. hind leg slices were chilly stored under two modified atmosphere packaging (map) environments (a: 15%o2/60%co2/25%n2, b: 15%o2/30%co2/55%n2) and vacuum packaging (v). only e. coli was reduced between 0.72-1.25 log cfu/g from day 1 to day 4 by the combined use of map/v, chilling storage and the growth of native lactic acid bacteria. however, l. monocytogenes was not inhibited by the a ... | 2015 | 26298670 |
| control of shigatoxin-producing escherichia coli in cheese by dairy bacterial strains. | bio-preservation could be a valuable way to control shigatoxin-producing escherichia coli (stec) in cheese. to this end, 41 strains were screened for their inhibitory potential on model cheese curd and on pasteurized and raw milk uncooked pressed cheeses. strains of lactococcus lactis, lactococcus garvieae, leuconostoc pseudomesenteroides, leuconostoc citreum, lactobacillus sp, carnobacterium mobile, enterococcus faecalis, enterococcus faecium, macrococcus caseolyticus and hafnia alvei reduced s ... | 2016 | 26678131 |
| identification and antimicrobial activity detection of lactic acid bacteria isolated from corn stover silage. | a total of 59 lactic acid bacteria (lab) strains were isolated from corn stover silage. according to phenotypic and chemotaxonomic characteristics, 16s ribosomal dna (rdna) sequences and reca gene polymerase chain reaction amplification, these lab isolates were identified as five species: lactobacillus (l.) plantarum subsp. plantarum, pediococcus pentosaceus, enterococcus mundtii, weissella cibaria and leuconostoc pseudomesenteroides, respectively. those strains were also screened for antimicrob ... | 2015 | 25924957 |