| taxonomic study of the lactobacillus acidophilus group, with recognition of lactobacillus gallinarum sp. nov. and lactobacillus johnsonii sp. nov. and synonymy of lactobacillus acidophilus group a3 (johnson et al. 1980) with the type strain of lactobacillus amylovorus (nakamura 1981). | biochemical properties and dna-dna reassociation studies of lactobacillus acidophilus strains isolated from humans and animals indicate that these include six genomospecies. two new species can be differentiated from the established species of the genus lactobacillus: l. gallinarum sp. nov. (type strain, atcc 33199) and l. johnsonii sp. nov. (type strain, atcc 33200). furthermore, it was clarified that l. acidophilus group a3 (johnson et al. 1980) is synonymous with l. amylovorus. | 1992 | 1503977 |
| mesentericin y105 gene clusters in leuconostoc mesenteroides y105. | because of their potential usefulness as natural food preservatives, increased interest has focused on bacteriocins from lactic acid bacteria. mesentericin y105 is a small non-lantibiotic bacteriocin (class ii) encoded within a 35 kb plasmid from leuconostoc mesenteroides y105 and it is active against listeria monocytogenes. using reverse genetic methodologies, an 8 kb draii fragment has been cloned that contains the mesentericin y105 structural gene, mesy, which encodes a precursor of the bacte ... | 1995 | 7551032 |
| utilization of the leucocin a export system in leuconostoc gelidum for production of a lactobacillus bacteriocin. | the lactacin f complex, composed of lafa and lafx peptides, is produced by lactobacillus johnsonii vpi 11088 (atcc 11506) and is active against various lactobacilli and enterococcus faecalis. the genetic determinants encoding the lactacin f peptides, lafa and lafx, are organized in a chromosomal operon comprised of genes lafa, lafx, and orfz. the lactacin f operon was introduced into leuconostoc (lc.) gelidum ual187-22 which produces leucocin a. leucocin a, a plasmid-encoded bacteriocin, inhibit ... | 1995 | 7557315 |
| evaluation of random amplified polymorphic dna (rapd)-pcr as a method to differentiate lactobacillus acidophilus, lactobacillus crispatus, lactobacillus amylovorus, lactobacillus gallinarum, lactobacillus gasseri, and lactobacillus johnsonii. | the technique random amplified polymorphic dna (rapd)-pcr was evaluated as a method to differentiate lactobacillus acidophilus, lactobacillus crispatus, lactobacillus amylovorus, lactobacillus gallinarum, lactobacillus gasseri, and lactobacillus johnsonii. representative strains, including the type of each species, were selected from different clusters obtained by numerical analysis of total soluble cell protein patterns. results obtained by rapd-pcr corresponded well with results obtained by nu ... | 1995 | 7606186 |
| heterologous expression of the lactacin f peptides by carnobacterium piscicola lv17. | the lactacin f complex, composed of lafa and lafx peptides, is produced by lactobacillus johnsonii vpi 11088 and is active against five other lactobacillus species and enterococcus faecalis. the genetic determinants encoding the lactacin f complex are organized in a 1-kb polycistronic operon which comprises three genes, lafa, lafx, and orfz (encoding the putative immunity protein). the lafa and lafx genes encode the bacteriocin precursors with n-terminal extensions characterized by a gly-gly-1*x ... | 1995 | 7747957 |
| isolation of a novel is3 group insertion element and construction of an integration vector for lactobacillus spp. | an insertion sequence (is) element from lactobacillus johnsonii was isolated, characterized, and exploited to construct an is-based integration vector. l. johnsonii nck61, a high-frequency conjugal donor of bacteriocin production (laf+) and immunity (lafr), was transformed to erythromycin resistance (emr) with the shuttle vector psa3. the nck61 conjugative functions were used to mobilize psa3 into a laf- lafs ems recipient. dna from the emr transconjugants transformed into escherichia coli mc106 ... | 1994 | 8071209 |
| expansion of bacteriocin activity and host range upon complementation of two peptides encoded within the lactacin f operon. | lactacin f is a membrane-active bacteriocin produced by lactobacillus johnsonii vpi11088 (laf+). the genetic determinants encoding lactacin f are organized in a 1-kb polycistronic operon composed of a promoter (p(laf)), three genes (lafa, lafx, and orfz), and a functional rho-independent transcription terminator. two laf- derivatives of vpi11088, designated nck64 and nck65, were characterized. nck64 contained a frameshift mutation in the lafa gene causing premature termination of translation. nc ... | 1994 | 8157592 |
| kinetic studies of the action of lactacin f, a bacteriocin produced by lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane. | the bacteriocin lactacin f is bactericidal against lactobacillus delbrueckii, lactobacillus helveticus, and enterococcus faecalis. activity against l. delbrueckii was recently shown to be dependent on two peptides, lafa and lafx, which are encoded within the lactacin f operon (t. r. klaenhammer, fems microbiol. rev. 12:39-87, 1993). it has been proposed that two peptides form an active lactacin f complex. in this study, the action of lactacin f against e. faecalis atcc 19443 and the effects of v ... | 1994 | 8161167 |
| molecular analysis of the lactacin f operon. | lactacin f is a nonlantibiotic, heat-stable, peptide bacteriocin produced by lactobacillus johnsonii vpi11088. molecular analysis of the lactacin f dna region characterized a small operon that codes for three open reading frames, designated lafa, lafx, and orfz. the peptide encoded by lafa, the lactacin f structural gene, was compared with various peptide bacteriocins from lactic acid bacteria, and similarities were identified in the amino and carboxy termini of the propeptides. site-directed mu ... | 1993 | 8285694 |
| positive selection, cloning vectors for gram-positive bacteria based on a restriction endonuclease cassette. | lactococcus lactis contains numerous restriction and modification (r/m) systems of different specificities. a novel iis type r/m system encoded by the llai operon has previously been characterized from the l. lactis conjugative plasmid ptr2030. the llai operon is composed of six genes: first, a small regulatory gene llaic precedes the methylase gene llaim. the following three genes, llai.1, llai.2, llai.3, are all essential for restriction endonuclease activity and are designed as the restrictio ... | 1996 | 8693025 |
| functional analysis of the gene encoding immunity to lactacin f, lafi, and its use as a lactobacillus-specific, food-grade genetic marker. | lactacin f is a two-component class ii bacteriocin produced by lactobacillus johnsonii vpi 11088. the laf operon is composed of the bacteriocin structural genes, lafa and lafx, and a third open reading frame, orfz. two strategies were employed to study the function of orfz. this gene was disrupted in the chromosome of nck64, a lafa729 lafx orfz derivative of vpi 11088. a disruption cassette consisting of orfz interrupted with a cat gene was cloned into psa3 and introduced into nck64. manipulatio ... | 1996 | 8953716 |
| effects of intrajejunal perfusion and chronic ingestion of lactobacillus johnsonii strain la1 on serum concentrations and jejunal secretions of immunoglobulins and serum proteins in healthy humans. | link-amster reported an increase in serum iga when healthy subjects ingested a fermented dairy product containing lactobacillus johnsonii la1. we aimed to assess the effects of la1 on the jejunal secretions and serum concentrations of total and specific immunoglobulins and proteins. | 1997 | 9207997 |
| characterisation and selection of probiotic lactobacilli for a preliminary minipig feeding trial and their effect on serum cholesterol levels, faeces ph and faeces moisture content. | three out of 297 lactobacillus strains isolated from pig faeces were selected for a feeding trial on account of their high bile-salt hydrolase (bsh) activity, bile-salt resistance, low ph tolerance and the production of antimicrobial substances. two strains were identified as lactobacillus johnsonii and one as lactobacillus reuteri by dna-dna hybridisation. l. johnsoniii bfe 1061 produced a bacteriocin active against a range of lactic acid bacteria (lab) and nonrelated bacteria including clostri ... | 1998 | 9600615 |
| identification of genes encoding conjugated bile salt hydrolase and transport in lactobacillus johnsonii 100-100. | cytosolic extracts of lactobacillus johnsonii 100-100 (previously reported as lactobacillus sp. strain 100-100) contain four heterotrimeric isozymes composed of two peptides, alpha and beta, with conjugated bile salt hydrolase (bsh) activity. we now report cloning, from the genome of strain 100-100, a 2,977-bp dna segment that expresses bsh activity in escherichia coli. the sequencing of this segment showed that it contained one complete and two partial open reading frames (orfs). the 3' partial ... | 1998 | 9721268 |
| the ability of probiotic bacteria to bind to human intestinal mucus. | human mucus was isolated from faecal samples of newborns, two and six month old infants and adults. the adhesion to this mucus by the bacteria mentioned below was assessed in vitro. depending on the age group: 44-46% of the applied lactobacillus gg, 23-30% of bifidobacterium lactis bb-12, 9-14% of lactobacillus johnsonii lj-1, 3-10% of lactobacillus salivarius lm2-118, lactobacillus crispatus m247, lactobacillus paracasei f19 and 2% of l. crispatus mu5 adhered. all the strains adhered better to ... | 1998 | 9809419 |
| phenotypic and phylogenetic characterization of a novel lactobacillus species from human sources: description of lactobacillus iners sp. nov. | eleven strains of a hitherto undescribed gram-positive, catalase-negative, facultatively anaerobic rod-shaped bacterium from human sources and medical care products were characterized by phenotypic and molecular taxonomic methods. the phenotypic properties of the bacterium were consistent with its assignment to the genus lactobacillus but it was readily distinguished from all currently described species of this genus by its biochemical characteristics and by sds-page analysis of its cellular pro ... | 1999 | 10028266 |
| cell surface-associated lipoteichoic acid acts as an adhesion factor for attachment of lactobacillus johnsonii la1 to human enterocyte-like caco-2 cells. | the influence of ph on the adhesion of two lactobacillus strains to caco-2 human intestinal cells was investigated. one strain, lactobacillus johnsonii la1, was adherent at any ph between 4 and 7. the other one, l. acidophilus la10, did not attach to this cell line under the same experimental conditions. on the basis of these results, we used the monoclonal antibody technique as a tool to determine differences on the surface of these bacteria and to identify a factor for adhesion. mice were immu ... | 1999 | 10049865 |
| modulation of nonspecific mechanisms of defense by lactic acid bacteria: effective dose. | the purpose of this study was to determine the effect of a fermented milk product containing lactobacillus johnsonii la1 (formerly known as lactobacillus acidophilus la1) on the phagocytic activity of peripheral blood leukocytes in healthy adult volunteers. furthermore, we sought to define the effective doses of the bacteria, examine the effect on respiratory burst activity, and, finally, examine the contribution made by the starter culture to the biological effects. volunteers were randomly dis ... | 1999 | 10342225 |
| the groesl chaperone operon of lactobacillus johnsonii. | the lactobacillus johnsonii vpi 11088 groesl operon was localized on the chromosome near the insertion element is1223. the operon was initially cloned as a series of three overlapping pcr fragments, which were sequenced and used to design primers to amplify the entire operon. the amplified fragment was used as a probe to recover the chromosomal copy of the groesl operon from a partial library of l. johnsonii vpi 11088 (nck88) dna, cloned in the shuttle vector ptrkh2. the 2,253-bp groesl fragment ... | 1999 | 10388700 |
| d-lactate dehydrogenase gene (ldhd) inactivation and resulting metabolic effects in the lactobacillus johnsonii strains la1 and n312. | lactobacillus johnsonii la1, a probiotic bacterium with demonstrated health effects, grows in milk, where it ferments lactose to d- and l-lactate in a 60:40% ratio. the d-lactate dehydrogenase (d-ldh) gene (ldhd) of this strain was isolated, and an in vitro-truncated copy of that gene was used to inactivate the genomic copy in two strains, la1 and n312, by gene replacement. for that, an 8-bp deletion was generated within the cloned ldhd gene to inactivate its function. the plasmid containing the ... | 1999 | 10473408 |
| characterization and production of amylovorin l471, a bacteriocin purified from lactobacillus amylovorus dce 471 by a novel three-step method. | the strongly hydrophobic bacteriocin amylovorin l471 from lactobacillus amylovorus dce 471 was isolated and purified to homogeneity from complex culture broth by a novel, rapid and simple three-step protocol including (i) ammonium sulphate precipitation, (ii) chloroform/methanol extraction/precipitation and (iii) reversed-phase hplc, the only chromatographic step involved. the molecular mass of the peptide was determined to be 4876.9 da by electrospray mass spectrometric analysis. n-terminal ami ... | 1999 | 10517609 |
| activation of human peripheral blood mononuclear cells by nonpathogenic bacteria in vitro: evidence of nk cells as primary targets. | the interaction of commensal bacteria with immunocompetent cells may occur in definite compartments of the mucosal immune system, as limited translocation through the epithelial barrier cannot be excluded. in this study the stimulation of human peripheral blood mononuclear cells and purified lymphocyte subsets by nonpathogenic gram-positive lactobacilli (lactobacillus johnsonii and lactobacillus sakei) and gram-negative escherichia coli was investigated. the various bacterial strains induced a d ... | 2000 | 10639443 |
| expression of clostridium thermocellum endoglucanase gene in lactobacillus gasseri and lactobacillus johnsonii and characterization of the genetically modified probiotic lactobacilli. | endoglucanase a from clostridium thermocellum resistant to pancreatic proteinase was selected out of a range of microbial cellulases expressed in lactobacilli. two lactobacillus-e. coli expression vectors, harboring the endoglucanase gene from c. thermocellum under the control of its own promoter (psd1) and the lactococcus lactis lac a promoter (psd2), were constructed separately. intestinal lactobacillus strains, l. gasseri and l. johnsonii, were electrotransformed with psd1 and psd2, and the s ... | 2000 | 10688695 |
| iron requirement of lactobacillus spp. in completely chemically defined growth media. | a completely chemically-defined growth medium, containing guanine, thymine, cytidine, 2'-deoxyadenosine and 2'-deoxyuridine as dna precursors, was developed for lactobacillus johnsonii, on the basis of statistically designed techniques suitable for other lactobacilli. particular focus was given to the nucleotide composition of different defined media, and to the specific nucleotide requirements of lact. johnsonii. most of the lactobacilli tested grew in a medium containing five free bases, four ... | 2000 | 10792529 |
| lactobacillus fornicalis sp. nov., isolated from the posterior fornix of the human vagina. | twelve strains isolated from the posterior fornix fluid of the human vagina were identified as lactobacillus johnsonii, lactobacillus acidophilus, lactobacillus gallinarum and lactobacillus crispatus based on numerical analyses of total soluble cell protein profiles and randomly amplified polymorphic dna (rapd)-pcr banding patterns. five strains grouped with the type strains of lactobacillus gasseri (dsm 20077t) and lactobacillus jensenii (dsm 20557t) at r > or = 0.83 in one protein profile clus ... | 2000 | 10843070 |
| non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. | intestinal epithelial cells (iec) are thought to participate in the mucosal defence against bacteria and in the regulation of mucosal tissue homeostasis. reactivity of iec to bacterial signals may depend on interactions with immunocompetent cells. to address the question of whether non-pathogenic bacteria modify the immune response of the intestinal epithelium, we co-cultivated enterocyte-like caco-2 cells with human blood leucocytes in separate compartments of transwell cultures. | 2000 | 10861268 |
| deoxyribonuclease activities in lactobacillus delbrueckii. | dnase activity was examined in the extracellular and subcellular fractions of six non-transformable strains belonging to lactobacillus delbrueckii subsp. lactis (l. lactis) and lactobacillus delbrueckii subsp. bulgaricus (l. bulgaricus) and compared with the activity present in lactobacillus johnsonii nck 65, a transformable strain of lactobacillus. in the extracellular fraction of the l. delbrueckii strains, a common protein band of 36 kda was detected, while a band of 29 kda was found in the s ... | 2000 | 10950192 |
| use of the dna sequence of variable regions of the 16s rrna gene for rapid and accurate identification of bacteria in the lactobacillus acidophilus complex. | the lactobacillus acidophilus complex includes lact. acidophilus, lactobacillus amylovorus, lactobacillus crispatus, lactobacillus gallinarum, lactobacillus gasseri and lactobacillus johnsonii. the objective of this work was to develop a rapid and definitive dna sequence-based identification system for unknown isolates of the lact. acidophilus complex. a approximately = 500 bp region of the 16s rrna gene, which contained the v1 and v2 variable regions, was amplified from the isolates by the poly ... | 2000 | 11021584 |
| lactobacillus johnsonii la1 shares carbohydrate-binding specificities with several enteropathogenic bacteria. | the carbohydrate-binding specificities of the probiotic lactic acid bacterium lactobacillus johnsonii la1 (a health-beneficial bacterial strain able to be incorporated into the human intestinal microflora) were investigated in vitro. first various soluble complex carbohydrates were tested as potential inhibitors of the strain adhesion onto caco-2 intestinal epithelial cells, and then bacterial binding to glycolipids immobilized on tlc plates was probed. two major carbohydrate-binding specificiti ... | 2000 | 11087711 |
| favourable effect of an acidified milk (lc-1) on helicobacter pylori gastritis in man. | the supernatant of lactobacillus johnsonii la1 culture was shown to be bactericidal and to have a partial, acid-independent suppressive effect on helicobacter pylori in humans. the aim of the present study was to investigate the effect of l. johnsonii la1-acidified milk (lc-1) on h. pylori infection. | 2001 | 11204805 |
| 16s ribosomal dna terminal restriction fragment pattern analysis of bacterial communities in feces of rats fed lactobacillus acidophilus ncfm. | 16s ribosomal dna terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics of lactobacillus acidophilus ncfm and discern bacterial populations that changed during feeding with ncfm. lactobacillus johnsonii and ruminococcus flavefaciens were tentatively identified as such bacterial populations. the presence of l. johnsonii was confirmed by isolation from feces. | 2001 | 11282651 |
| characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish. | the present study aimed to investigate the potential probiotic properties of six lactic acid bacteria (lab) intended for human use, lactobacillus rhamnosus atcc 53103, lactobacillus casei shirota, lactobacillus bulgaricus, l. rhamnosus lc 705, bifidobacterium lactis bb12, and lactobacillus johnsonii la1, and one for animal use, enterococcus faecium tehobak, for use as a fish probiotic. the strains for human use were specifically chosen since they are known to be safe for human use, which is of m ... | 2001 | 11375147 |
| inhibition of giardia intestinalis by extracellular factors from lactobacilli: an in vitro study. | the aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. the growth of giardia intestinalis strain wb, as well as the attachment to the human intestinal epithelial cell line caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. in addition, scanning electron microscopy and flow cytometric analysis were performed. the effect of spent culture supernatants from lactobaci ... | 2001 | 11679323 |
| genes encoding bile salt hydrolases and conjugated bile salt transporters in lactobacillus johnsonii 100-100 and other lactobacillus species. | lactobacillus johnsonii strain 100-100 expresses two antigenically distinct conjugated bile salt hydrolases (bsh), alpha and beta, that combine to form native homo- and heterotrimers. this paper reports characterization of loci within the genome that encode this capacity. a locus that encodes bsh beta (cbsh beta), a partial (cbst1) and a complete conjugated bile salt transporter (cbst2) was identified previously. dna sequence analysis at this locus was extended and revealed a complete orf for cb ... | 2001 | 11739773 |
| lipoteichoic acids from lactobacillus johnsonii strain la1 and lactobacillus acidophilus strain la10 antagonize the responsiveness of human intestinal epithelial ht29 cells to lipopolysaccharide and gram-negative bacteria. | intestinal epithelial cells (iecs) respond to lipopolysaccharide (lps) from gram-negative bacteria in the presence of the soluble form of cd14 (scd14), a major endotoxin receptor. since scd14 is also known to interact with gram-positive bacteria and their components, we looked at whether scd14 could mediate their effects on human iecs. to this end, we examined the production of proinflammatory cytokines following exposure of the iecs to specific gram-positive bacteria or their lipoteichoic acids ... | 2002 | 11895971 |
| sequence analysis of plbb1, a cryptic plasmid from lactobacillus delbrueckii subsp. bulgaricus. | the first report of the complete nucleotide sequence of a cryptic plasmid from lactobacillus delbrueckii subsp. bulgaricus (lactobacillus bulgaricus) is presented. the plasmid plbb1 consists of 6127 bp with a gc content of 44.8%. no ssdna was detected by hybridization experiments, which is consistent with the notion that plbb1 does not replicate by a rolling circle mechanism. a putative replication region of plbb1 was cloned and found to be functional in lactobacillus johnsonii and lactococcus l ... | 2002 | 11958563 |
| activation of human nk cells by staphylococci and lactobacilli requires cell contact-dependent costimulation by autologous monocytes. | nk cells are instrumental in innate immune responses, in particular for the early production of gamma interferon (ifn-gamma) and other cytokines necessary to control certain bacterial, parasitic, and viral infections. nk cell-mediated effector functions are controlled by a fine balance between distinct receptors mediating activating and inhibitory signals; however, little is known about activating receptors on nk cells and their corresponding ligands. several studies have shown that commensal la ... | 2002 | 11986274 |
| recombinant lactobacillus johnsonii as a mucosal vaccine delivery vehicle. | lactobacilli are considered to be safe organisms making them attractive as vehicles for oral vaccination. we report that lactobacillus johnsonii (lj) partially survived simulated gastric conditions in vitro, suggesting that it could be used as an oral vaccine delivery vehicle. in order to test this approach, we used the cell wall anchored proteinase prtb, isolated from lactobacillus delbrueckii subsp. bulgaricus as a model antigen. using a new vector system, we demonstrated expression of both pr ... | 2002 | 12126902 |
| effect of lactobacillus johnsonii la1 and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis. | probiotics and antioxidants could be alternatives to antibiotics in the prevention of bacterial infections in cirrhosis. the aim of the present study was to determine the effect of lactobacillus johnsonii la1 and antioxidants on intestinal flora, endotoxemia, and bacterial translocation in cirrhotic rats. | 2002 | 12217598 |
| identification and characterization of novel surface proteins in lactobacillus johnsonii and lactobacillus gasseri. | we have identified and sequenced the genes encoding the aggregation-promoting factor (apf) protein from six different strains of lactobacillus johnsonii and lactobacillus gasseri. both species harbor two apf genes, apf1 and apf2, which are in the same orientation and encode proteins of 257 to 326 amino acids. multiple alignments of the deduced amino acid sequences of these apf genes demonstrate a very strong sequence conservation of all of the genes with the exception of their central regions. n ... | 2002 | 12450842 |
| specific identification and molecular typing analysis of lactobacillus johnsonii by using pcr-based methods and pulsed-field gel electrophoresis. | a fast and reliable multiplex-pcr assay was established to identify the species lactobacillus johnsonii. two opposing rrna gene-targeted primers have been designed for this specific pcr detection. specificity was verified with dna samples isolated from different lactic acid bacteria. out of 47 lactobacillus strains isolated from different environments, 16 were identified as l. johnsonii by pcr. the same set of strains was investigated with five alternative molecular typing methods: enterobacteri ... | 2002 | 12480097 |
| lytic and nonlytic mechanism of inactivation of gram-positive bacteria by lysozyme under atmospheric and high hydrostatic pressure. | a different behavior was observed in three gram-positive bacteria exposed to hen egg white lysozyme by plate counts and phase-contrast microscopy. the inactivation of lactobacillus johnsonii was accompanied by spheroplast formation, which is an indication of peptidoglycan hydrolysis. staphylococcus aureus was resistant to lysozyme and showed no signs of peptidoglycan hydrolysis, and listeria innocua was inactivated and showed indications of cell leakage but not of peptidoglycan hydrolysis. under ... | 2002 | 12495010 |
| divergent patterns of colonization and immune response elicited from two intestinal lactobacillus strains that display similar properties in vitro. | lactobacilli derived from the endogenous flora of normal donors are being increasingly used as probiotics in functional foods and as vaccine carriers. however, a variety of studies done with distinct strains of lactobacilli has suggested heterogeneous and strain-specific effects. to dissect this heterogeneity at the immunological level, we selected two strains of lactobacilli that displayed similar properties in vitro and studied their impact on mucosal and systemic b-cell responses in monoxenic ... | 2003 | 12496193 |
| suppressive effect of frequent ingestion of lactobacillus johnsonii la1 on helicobacter pylori colonization in asymptomatic volunteers. | | 2003 | 12697639 |
| integration and distribution of lactobacillus johnsonii prophages. | in lactobacillus johnsonii strain ncc533, two prophages were integrated into trna genes and one was disrupted by integration. in a survey, the prophages were restricted to strains sharing an essentially identical restriction pattern. microarray analysis showed that the prophage dna represents about 50% of the ncc533 strain-specific dna. | 2003 | 12867471 |
| effect of the ingestion of a dietary product containing lactobacillus johnsonii la1 on helicobacter pylori colonization in children. | dietary components such as vegetable or probiotic microorganisms have been proposed as an alternative solution to decrease helicobacter pylori colonization in at-risk populations. some strains of lactic acid bacteria have been shown to exert bacteriostatic or bactericidal effects against h. pylori in in vitro and in vivo models of infection by this pathogen. we investigated whether regular ingestion of a dietary product containing lactobacillus johnsonii la1 or l. paracasei st11 would interfere ... | 2003 | 12921879 |
| effect of probiotic bacteria on induction and maintenance of oral tolerance to beta-lactoglobulin in gnotobiotic mice. | in this study, the effect of lactobacillus paracasei (ncc 2461), lactobacillus johnsonii (ncc 533) and bifidobacterium lactis bb12 (ncc 362) on the induction and maintenance of oral tolerance to bovine beta-lactoglobulin (blg) was investigated in mice. germfree mice were monocolonized with one of the three strains before oral administration of whey protein to induce tolerance. mice were then injected with blg and sacrificed 28 or 50 days after whey protein feeding for humoral and cellular respon ... | 2003 | 12965905 |
| favourable effect of regular intake of fermented milk containing lactobacillus johnsonii on helicobacter pylori associated gastritis. | lactobacillus johnsonii (lj1) had an in vitro and in vivo inhibitory effect on helicobacter pylori. fermented milk containing lj1 (lc1), coadministered with antibiotics had a favourable effect on h. pylori gastritis. | 2003 | 14535874 |
| detection and identification of lactobacillus species in crops of broilers of different ages by using pcr-denaturing gradient gel electrophoresis and amplified ribosomal dna restriction analysis. | the microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using pcr combined with denaturing gradient gel electrophoresis (pcr-dgge) and selective bacteriological culture of lactobacilli followed by amplified ribosomal dna restriction analysis (ardra). the birds were raised under conditions similar to those used in commercial broiler production. lactobacilli predominated and attained populations of 10(8) to 10(9) cfu per gram of crop contents. many of th ... | 2003 | 14602636 |
| in vivo characterization of lactobacillus johnsonii fi9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry. | to test the efficacy of lactobacillus johnsonii fi9785 in reducing the colonization and shedding of salmonella enterica serotype enteritidis, escherichia coli o78:k80 and clostridium perfringens in poultry. | 2004 | 14962040 |
| the genome sequence of the probiotic intestinal bacterium lactobacillus johnsonii ncc 533. | lactobacillus johnsonii ncc 533 is a member of the acidophilus group of intestinal lactobacilli that has been extensively studied for their "probiotic" activities that include, pathogen inhibition, epithelial cell attachment, and immunomodulation. to gain insight into its physiology and identify genes potentially involved in interactions with the host, we sequenced and analyzed the 1.99-mb genome of l. johnsonii ncc 533. strikingly, the organism completely lacked genes encoding biosynthetic path ... | 2004 | 14983040 |
| l-alanine auxotrophy of lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches. | using a chemically defined medium without l-alanine, lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. a comparative genetic analysis showed that all known genes involved in l-alanine biosynthesis are absent from the genome of l. johnsonii. this auxotrophy was complemented by heterologous expression of the bacillus subtilis l-alanine dehydrogenase. | 2004 | 15006820 |
| the prophages of lactobacillus johnsonii ncc 533: comparative genomics and transcription analysis. | two non-inducible, but apparently complete prophages were identified in the genome of the sequenced lactobacillus johnsonii strain ncc 533. the 38- and 40-kb-long prophages lj928 and lj965 represent distinct lineages of sfi11-like pac-site siphoviridae unrelated at the dna sequence level. the deduced structural proteins from lj928 demonstrated aa sequence identity with lactococcus lactis phage tp901-1, while lj965 shared sequence links with streptococcus thermophilus phage o1205. with the except ... | 2004 | 15016546 |
| cell surface-associated elongation factor tu mediates the attachment of lactobacillus johnsonii ncc533 (la1) to human intestinal cells and mucins. | the aim of this work was to identify lactobacillus johnsonii ncc533 (la1) surface molecules mediating attachment to intestinal epithelial cells and mucins. incubation of caco-2 intestinal epithelial cells with an l. johnsonii la1 cell wall extract led to the recognition of elongation factor tu (ef-tu) as a novel la1 adhesin-like factor. the presence of ef-tu at the surface of la1 was confirmed by analysis of purified outer surface protein extract by immunoblotting experiments, by electron micros ... | 2004 | 15039339 |
| cbst2 from lactobacillus johnsonii 100-100 is a transport protein of the major facilitator superfamily that facilitates bile acid antiport. | we previously identified two conjugated bile acid transporters, cbst1 and cbst2, in lactobacillus johnsonii 100-100 and lactobacillus acidophilus ks-13 that are gene duplicates encoded in tandem with a conjugated bile salt hydrolase (bsh) [elkins and savage, j. bacteriol. 180:4344-4349, 1998; elkins et al., microbiology 147: 3403-3412, 2001]. cbst2 from 100-100 was shown to increase taurocholic acid (tca) uptake in escherichia coli; however, higher levels were achieved when an extracellular fact ... | 2003 | 15044826 |
| bifidobacterium lactis dsm 10140: identification of the atp (atpbefhagdc) operon and analysis of its genetic structure, characteristics, and phylogeny. | the atp operon is highly conserved among eubacteria, and it has been considered a molecular marker as an alternative to the 16s rrna gene. pcr primers were designed from the consensus sequences of the atpd gene to amplify partial atpd sequences from 12 bifidobacterium species and nine lactobacillus species. all pcr products were sequenced and aligned with other atpd sequences retrieved from public databases. genes encoding the subunits of the f(1)f(0)-atpase of bifidobacterium lactis dsm 10140 ( ... | 2004 | 15128574 |
| identification and characterization of the novel lysm domain-containing surface protein sep from lactobacillus fermentum br11 and its use as a peptide fusion partner in lactobacillus and lactococcus. | examination of supernatant fractions from broth cultures of lactobacillus fermentum br11 revealed the presence of a number of proteins, including a 27-kda protein termed sep. the amino-terminal sequence of sep was determined, and the gene encoding it was cloned and sequenced. sep is a 205-amino-acid protein and contains a 30-amino-acid secretion signal and has overall homology (between 39 and 92% identity) with similarly sized proteins of lactobacillus reuteri, enterococcus faecium, streptococcu ... | 2004 | 15184172 |
| expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity. | in living organisms, exposure to oxygen provokes oxidative stress. a widespread mechanism for protection against oxidative stress is provided by the antioxidant enzymes: superoxide dismutases (sods) and hydroperoxidases. generally, these enzymes are not present in lactobacillus spp. in this study, we examined the potential advantages of providing a heterologous sod to some of the intestinal lactobacilli. thus, the gene encoding the manganese-containing sod (soda) was cloned from streptococcus th ... | 2004 | 15294805 |
| identification, characterisation and specificity of a cell wall lytic enzyme from lactobacillus fermentum br11. | screening of a genomic library with an antiserum raised against whole lactobacillus fermentum br11 cells identified a clone expressing an immunoreactive 37-kda protein. analysis of the 3010-bp dna insert contained within the clone revealed four open reading frames (orfs). one orf encodes lysa, a 303 amino acid protein which has up to 35% identity with putative endolysins from prophages lj928 and lj965 from lactobacillus johnsonii and lp1 and lp2 from lactobacillus plantarum as well as with the e ... | 2004 | 15336396 |
| lactobacillus paracasei normalizes muscle hypercontractility in a murine model of postinfective gut dysfunction. | the effects of probiotics on gut dysfunction in postinfective irritable bowel syndrome are unknown. we tested whether probiotics influence persistent muscle hypercontractility in mice after recovery from infection with trichinella spiralis and analyzed the underlying mechanisms. | 2004 | 15362038 |
| isolation and characterization of anti-salmonella lactic acid bacteria from the porcine gastrointestinal tract. | to identify lactic acid bacteria (lab) of porcine intestinal origin with anti-salmonella activity. | 2004 | 15482434 |
| the complete genomes of lactobacillus plantarum and lactobacillus johnsonii reveal extensive differences in chromosome organization and gene content. | the first comprehensive comparative analysis of lactobacilli was done by comparing the genomes of lactobacillus plantarum (3.3 mb) and lactobacillus johnsonii (2.0 mb). l. johnsonii is predominantly found in the gastrointestinal tract, while l. plantarum is also found on plants and plant-derived material, and is used in a variety of industrial fermentations. the l. plantarum and l. johnsonii chromosomes have only 28 regions with conservation of gene order, totalling about 0.75 mb; these regions ... | 2004 | 15528649 |
| intranasal immunisation using recombinant lactobacillus johnsonii as a new strategy to prevent allergic disease. | we have previously demonstrated the induction of a specific anti-ige response in vivo by parenteral immunisation of rhesus monkeys using short ige mimotopes or an anti-idiotypic antibody mimicking an ige epitope. such specific anti-ige responses may be of clinical benefit for atopic patients. in this study, we examined the potential for a more convenient therapy via mucosal immunisation using live recombinant lactobacillus johnsonii (lj) as a vaccine delivery vehicle. either an anti-idiotypic sc ... | 2005 | 15629355 |
| lactobacillus johnsonii la1 antagonizes giardia intestinalis in vivo. | this study describes the in vivo activity of lactobacillus johnsonii la1 (ncc533) in giardia intestinalis-infected gerbils (meriones unguiculatus). daily administration of lactobacilli in the drinking water from 7 days before inoculation with giardia trophozoites efficiently prevented g. intestinalis strain wb clone c6 from infecting gerbils. more specifically, shedding of fecal giardia antigens (gsa65 protein) was diminished in the la1-treated group, and resolution of infection was observed by ... | 2005 | 15664978 |
| a dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics. | a dynamic model of the human upper gastrointestinal (gi) tract was designed to better simulate conditions of ingestion and digestion, by including a food matrix as part of the model design. the dynamic model consisted of two reactors maintained at 37 degrees c, one simulating stomach conditions and the other simulating duodenum conditions. the model was tested by comparing survival of bacteria isolated from humans (bifidobacterium infantis, lactobacillus johnsonii, lactobacillus rhamnosus, and l ... | 2005 | 15808363 |
| antimicrobial potential of probiotic lactic acid bacteria. | antibacterial activity is an important characteristic of probiotics. a possible way to achieve this antibacterial activity is through the production of bacteriocins. this study shows that the commercial probiotic strains lactobacillus johnsonii la1 and lactobacillus casei yit 9029 produce bacteriocins. the nature of the antimicrobial compound could be derived from its behaviour: a narrow inhibitory spectrum (only active against closely related strains), loss of activity when treated with protein ... | 2001 | 15954651 |
| modulation of the fecal microbiota by the intake of a lactobacillus johnsonii la1-containing product in human volunteers. | lactobacillus johnsonii la1 (la1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. this study evaluates how the ingestion of different amounts of la1 influences the main bacterial populations of the fecal microbiota. eight asymptomatic volunteers participated in the study. after a basal period, they ingested daily 100 ml of a product containing 10(8) cfu ml(-1) of la1 during the first week, 200 ml during the second week a ... | 2005 | 15970400 |
| 13c- and 15n-incorporation of doubly stable isotope labelled lactobacillus johnsonii in humans. | in the present study, lactobacillus johnsonii (la1) doubly labelled with 15n and 13c (dlla1) was used to follow the metabolic fate of orally administered dlla1 in humans. | 2005 | 16034363 |
| effects of intraduodenal injection of lactobacillus johnsonii la1 on renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats. | previously, it was shown that milk fermented with lactic acid bacteria lowers blood pressure, suggesting that metabolites or components of the bacteria have hypotensive action. to examine whether one of lactobacilli, lactobacillus johnsonii la1 (ljla1), a probiotic strain adhesive onto intestinal epithelial cells, or its metabolite has hypotensive action, and if so the mechanism of action, we determined the effects of intraduodenal injection of ljla1 on blood pressure (bp) and the activity of au ... | 2005 | 16118039 |
| characterisation of a novel plasmid p9785s from lactobacillus johnsonii fi9785. | lactobacillus johnsonii fi9785, a strain originally isolated from poultry gastrointestinal tract for its probiotic function as a competitive excluder of pathogens, was found to contain two cryptic plasmids of 3.5 and 25.6 kb. nucleotide sequence analysis of the entire small plasmid, designated p9785s (3471 bp), indicated a g+c content of 35.8%, and revealed two open reading frames (orfs). the product of orf1 exhibited similarity to the relaxases of mobilizable plasmids, whereas the product of or ... | 2005 | 16122563 |
| ph-, lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against salmonella enterica serovar typhimurium. | the mechanism(s) underlying the antibacterial activity of probiotic lactobacillus strains appears to be multifactorial and includes lowering of the ph and the production of lactic acid and of antibacterial compounds, including bacteriocins and nonbacteriocin, non-lactic acid molecules. addition of dulbecco's modified eagle's minimum essential medium to the incubating medium delays the killing activity of lactic acid. we found that the probiotic strains lactobacillus johnsonii la1, lactobacillus ... | 2005 | 16204515 |
| effects of culture conditions on the growth and auto-aggregation ability of vaginal lactobacillus johnsonii crl 1294. | to evaluate the effects of different physico-chemical factors on the growth and auto-aggregating ability of vaginal lactobacillus johnsonii crl 1294. | 2005 | 16313411 |
| comparative and functional analysis of the rrna-operons and their trna gene complement in different lactic acid bacteria. | the complete genome sequences of the lactic acid bacteria (lab), lactobacillus plantarum, lactococcus lactis, and lactobacillus johnsonii were used to compare location, sequence, organisation, and regulation of the ribosomal rna (rrn) operons. all rrn operons of the examined lab diverge from the origin of replication, which is compatible with their efficient expression. all operons show a common organisation of 5'-16s-23s-5s-3' structure, but differ in the number, location and specificity of the ... | 2006 | 16338113 |
| lactobacillus johnsonii la1 attenuates helicobacter pylori-associated gastritis and reduces levels of proinflammatory chemokines in c57bl/6 mice. | in clinical settings, lactobacillus johnsonii la1 administration has been reported to have a favorable effect on helicobacter pylori-associated gastritis, although the mechanism remains unclear. we administered, continuously through the water supply, live la1 to h. pylori-infected c57bl/6 mice and followed colonization, the development of h. pylori-associated gastritis in the lamina propria, and the levels of proinflammatory chemokines macrophage inflammatory protein 2 (mip-2) and keratinocyte-d ... | 2005 | 16339060 |
| groel of lactobacillus johnsonii la1 (ncc 533) is cell surface associated: potential role in interactions with the host and the gastric pathogen helicobacter pylori. | heat shock proteins of the groel or hsp60 class are highly conserved proteins essential to all living organisms. even though groel proteins are classically considered intracellular proteins, they have been found at the surface of several mucosal pathogens and have been implicated in cell attachment and immune modulation. the purpose of the present study was to investigate the groel protein of a gram-positive probiotic bacterium, lactobacillus johnsonii la1 (ncc 533). its presence at the bacteria ... | 2006 | 16368998 |
| ineffectiveness of lactobacillus johnsonii la1 for prophylaxis of postoperative recurrence in crohn's disease: a randomised, double blind, placebo controlled getaid trial. | early endoscopic recurrence is frequent after intestinal resection for crohn's disease. bacteria are involved, and probiotics may modulate immune responses to the intestinal flora. here we tested the probiotic strain lactobacillus johnsonii la1 in this setting. | 2006 | 16377775 |
| improvement of the human intestinal flora by ingestion of the probiotic strain lactobacillus johnsonii la1. | to exert beneficial effects for the host, for example, improving the intestinal microflora, a probiotic must reach the intestine as a viable strain. these properties must be demonstrated by in vitro as well as in vivo methods. however, only a few well-designed human clinical studies have shown these properties. lactobacillus johnsonii la1 has been shown to give many beneficial effects for the host, but it is unclear whether a viable strain of l. johnsonii la1 has the effect of improving host int ... | 2006 | 16469146 |
| postoperative maintenance therapy for inflammatory bowel disease. | this review will highlight the knowledge gained from studies published in the year 2005 on maintenance treatment after surgery for inflammatory bowel diseases. | 2006 | 16760753 |
| effects of probiotic or prebiotic supplemented milk formulas on fecal microbiota composition of infants. | the aim of the study was to evaluate whether supplementation of milk-formulas with prebiotic fructo-oligosaccharides or a probiotic, lactobacillus johnsonii la1 (la1), could modulate the composition of the fecal microbiota of formula-fed infants, compared to breastfed (bf) infants. ninety infants close to 4 months of age were randomized into one of three groups to be blindly assigned to receive for 13 weeks: a) an infant formula (control), b) the same formula with fructo-oligosaccharides (prebio ... | 2006 | 16837430 |
| effects of the probiotic strain lactobacillus johnsonii strain la1 on autonomic nerves and blood glucose in rats. | oral administration of lactobacillus casei reportedly reduces blood glucose concentrations in a non-insulin-dependent diabetic kk-ay mouse model. in order to determine if other lactobacillus strains affect glucose metabolism, we evaluated the effect of the probiotic strain lactobacillus johnsonii la1 (ljla1) strain on glucose metabolism in rats. oral administration of ljla1 via drinking water for 2 weeks inhibited the hyperglycemia induced by intracranial injection of 2-deoxy-d-glucose (2dg). we ... | 2006 | 16893554 |
| the in vitro effect of hydrogen peroxide on vaginal microbial communities. | this study presents a series of experiments carried out in order to elucidate the role of h2o2 in antimicrobial activity of lactobacilli. vaginal swabs were collected from 60 premenopausal women and checked for ph and nugent score, and lactobacillus species were cultured, phenotyped and genotyped. the main outcome measures involved: (1) species of vaginal lactobacilli most effective in liberating h2o2, (2) minimal microbicidal concentrations of added h2o2, (3) kinetics of h2o2 liberation in rela ... | 2006 | 16965352 |
| molecular analysis of a chromosome-carried erm(b) gene and its flanking insertion points in lactobacillus johnsonii g41. | an erm(b) gene carried on the lactobacillus johnsonii g41 chromosome and the upstream and downstream regions were fully sequenced. apparently, a 1,495-bp segment of pre25 from enterococcus faecalis carrying the erm(b) gene became inserted, by an unknown mechanism, into the l. johnsonii chromosome. | 2006 | 17000744 |
| supplementation with oral probiotic bacteria maintains cutaneous immune homeostasis after uv exposure. | probiotic bacteria have been shown to modulate the immune system of the gut and protect against infectious and inflammatory diseases of the gastro-intestinal tract. ultraviolet radiation (uvr) is known to alter the cutaneous and systemic immune systems implicated in the development of skin tumors. in this study we investigated whether oral probiotics are able to modulate the immune system of the skin using hairless skh:hr1 mice exposed to an acute dose of uvr. we show that nutritional supplement ... | 2006 | 17101471 |
| influence of intensive and extensive breeding on lactic acid bacteria isolated from gallus gallus domesticus ceca. | in the present study, lactic acid bacteria (lab) from the cecum of chickens bred either under intensive (commercial broilers) or extensive (free-range) conditions were isolated, identified and some of their probiotic characteristics determined. the lab identified by 16s-23s rrna pcr-ardra were mainly of lactobacillus species and to a lesser extent of enterococcus spp. for all animals. free-range chickens showed a higher presence of lactobacillus acidophilus while lactobacillus reuteri and lactob ... | 2007 | 17113730 |
| similarity and differences in the lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics. | a set of lactobacilli were investigated by polyphasic analysis. multilocus sequence analysis, dna typing, microarray analysis, and in silico whole-genome alignments provided a remarkably consistent pattern of similarity within the lactobacillus acidophilus complex. on microarray analysis, 17 and 5% of the genes from lactobacillus johnsonii strain ncc533 represented variable and strain-specific genes, respectively, when tested against four independent isolates of l. johnsonii. when projected on t ... | 2007 | 17142402 |
| gram-negative bacteria aggravate murine small intestinal th1-type immunopathology following oral infection with toxoplasma gondii. | oral infection of susceptible mice with toxoplasma gondii results in th1-type immunopathology in the ileum. we investigated gut flora changes during ileitis and determined contributions of gut bacteria to intestinal inflammation. analysis of the intestinal microflora revealed that ileitis was accompanied by increasing bacterial load, decreasing species diversity, and bacterial translocation. gram-negative bacteria identified as escherichia coli and bacteroides/prevotella spp. accumulated in infl ... | 2006 | 17142781 |
| multicenter randomized-controlled clinical trial of probiotics (lactobacillus johnsonii, la1) on early endoscopic recurrence of crohn's disease after lleo-caecal resection. | seventy percent of crohn's disease (cd) patients exhibit anastomotic recurrence within 1 year after ileo-caecal surgery. recent clinical trials suggest the beneficial use of probiotics in the control of intestinal inflammation in pouchitis and ulcerative colitis. this study is a multicenter clinical trial evaluating the efficacy of an oral administration of the probiotic lal on early post-operative endoscopic recurrence of cd. | 2007 | 17206696 |
| adhesive and chemokine stimulatory properties of potentially probiotic lactobacillus strains. | five lactobacillus plantarum strains and two lactobacillus johnsonii strains, stemming either from african traditionally fermented milk products or children's feces, were investigated for probiotic properties in vitro. the relationship between the hydrophobic-hydrophilic cell surface and adhesion ability to ht29 intestinal epithelial cells was investigated, and results indicated that especially the l. johnsonii strains, which exhibited both hydrophobic and hydrophilic surface characteristics, ad ... | 2007 | 17265871 |
| growth of probiotic bacteria and bifidobacteria in a soy yogurt formulation. | soy beverage and cows' milk yogurts were produced with steptococcus thermophilus (atcc 4356) and lactobacillus delbrueckii subsp. bulgaricus (im 025). the drop in ph during fermentation was faster in the soy beverage than in cows' milk, but the final ph values were similar. yogurts were prepared with a yogurt starter in conjunction with either the probiotic bacteria lactobacillus johnsonii ncc533 (la-1), lactobacillus rhamnosus atcc 53103 (gg) or human derived bifidobacteria. the presence of the ... | 2007 | 17292991 |
| oral treatment with probiotic lactobacillus johnsonii ncc533 (la1) for a specific part of the weaning period prevents the development of atopic dermatitis induced after maturation in model mice, nc/nga. | the inhibitory effect of probiotic bacteria on atopic dermatitis has been shown in human infants, but the mechanism is still unclear. | 2007 | 17300240 |
| lactobacillus plantarum for oral peptide delivery. | to evaluate strains of lactobacilli for their ability to persist and secrete heterologous protein in the oral cavity. | 2007 | 17311639 |
| the metabolic fate of doubly stable isotope labelled heat-killed lactobacillus johnsonii in humans. | in this study, heat-killed lactobacillus johnsonii (la1), doubly labelled with (13)c and (15)n (hk-dlla1), was used to follow the metabolic fate after oral administration in humans. | 2008 | 17356556 |
| effect of lactobacillus johnsonii la1 on immune function and serum albumin in aged and malnourished aged mice. | protein-energy malnutrition (pem) is a serious nutritional problem that causes immune dysfunction in elderly people. probiotic lactic acid bacteria may potentially modify immunity; however, there is little evidence to elucidate the influence of these bacteria on pem in the elderly. | 2007 | 17367996 |
| identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control salmonella enteritidis. | to select lactobacillus strains from laying hens for potential use as probiotic to control salmonella enteritidis infection. | 2007 | 17381753 |
| isolation and molecular characterization of antibiotic-resistant lactic acid bacteria from poultry and swine meat products. | the transfer via the food chain from animals to humans of microbes that are resistant to antimicrobial agents is of increasing concern. to determine the contributions of nonpathogenic microflora to the occurrence and spread of antibiotic resistance (ar) genes in the food chain, 123 lactic acid bacteria were isolated from 29 samples of raw and processed pork and chicken meat products that had previously tested positive for one or more ar genes that encode clinically relevant ars: tet(m), tet(o), ... | 2007 | 17388042 |
| primary administration of lactobacillus johnsonii ncc533 in weaning period suppresses the elevation of proinflammatory cytokines and cd86 gene expressions in skin lesions in nc/nga mice. | the administration of probiotic lactic acid bacteria (lab) has been studied for its potential to prevent atopic dermatitis (ad). the objective of this study was to assess the inhibitory mechanism of a skin lesion by lab using an experimental model that we previously demonstrated in nc/nga mice. lactobacillus johnsonii ncc533 (la1) was administered orally to the la1 group from 20 to 22 days after birth, while phosphate-buffered saline was given to the control group. after the induction of skin le ... | 2007 | 17425659 |
| improvement of nutritional status and incidence of infection in hospitalised, enterally fed elderly by feeding of fermented milk containing probiotic lactobacillus johnsonii la1 (ncc533). | probiotics have potential to improve host immunity; however, there is less evidence showing their efficacy against infections and nutritional status in the elderly. we conducted a double-blinded feeding trial in the elderly to elucidate the effect of fermented milk containing lactobacillus johnsonii la1 (lc1) on infections and nutritional status. twenty-four completely enterally fed elderly in-patients aged over 70 years were randomly assigned into two groups. all subjects were administered 3768 ... | 2007 | 17617944 |
| synergy between lactobacillus paracasei and its bacterial products to counteract stress-induced gut permeability and sensitivity increase in rats. | stressful events result in the alteration of gut permeability and sensitivity. lactobacillus paracasei ncc2461 (lpa) therapy prevents antibiotic-induced visceral hyperalgesia in mice. this study aimed at evaluating the influence of 3 probiotic strains: bifidobacterium lactis ncc362, lactobacillus johnsonii ncc533, and lpa on stress-mediated alterations of colorectal hyperalgesia, on gut paracellular permeability and whether bacteria and/or bacterial products present in the spent culture medium ( ... | 2007 | 17634262 |
| extensive horizontal transfer of core genome genes between two lactobacillus species found in the gastrointestinal tract. | while genes that are conserved between related bacterial species are usually thought to have evolved along with the species, phylogenetic trees reconstructed for individual genes may contradict this picture and indicate horizontal gene transfer. individual trees are often not resolved with high confidence, however, and in that case alternative trees are generally not considered as contradicting the species tree, although not confirming it either. here we conduct an in-depth analysis of 401 prote ... | 2007 | 17708761 |
| the lactobacillus plantarum strain aca-dc287 isolated from a greek cheese demonstrates antagonistic activity in vitro and in vivo against salmonella enterica serovar typhimurium. | the purpose of this study was to investigate the antibacterial activity of the xynotyri cheese isolate lactobacillus plantarum aca-dc287 using a set of in vitro and in vivo assays. | 2007 | 17714399 |