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immunological relationships among transaldolases in the genus bifidobacterium.antisera were prepared against electrophoretically homogeneous transaldolase (dihydroxyacetone transferase, e.c. 2.2.1.2.) of bifidobacterium thermophilum (b. ruminale) ru326 (atcc25866), b. cuniculi ra93 (atcc27916) and b. 'minimum' (homology group) f392 (atcc 27538). crude extracts of eighty six strains previously assigned to twenty one species of the genus bifidobacterium on the basis of deoxyribonouclelic acid (dna) homology (dna-dna hybridization), were compared by double diffusion tests on ...197995384
presence of bifidobacteria in the rumen of calves fed different rations.a study was made on the numbers and species of bifidobacteria present in the rumen of calves fed high-roughage and high-concentrate diets. with the roughage ration the bifidobacteria were not detectable in a 10(-3) dilution, whereas with the concentrate ration their number was high, usually in the order of 10(8) to 10(9)/ml of rumen fluid. the species most represented, identified by means of deoxyribonucleic acid-deoxyribonucleic acid hybridization tests, included bifidobacterium ruminale, bifid ...1976984825
adhesion of bacteria to the cecal mucosal surface of conventional and germ-free chickens infected with eimeria tenella.when salmonella typhimurium and clostridium perfringens were tested in conventional chickens, larger numbers of s typhimurium and c perfringens adhered to eimeria tenella-infected ceca than to uninfected ceca. in germ-free chickens, s typhimurium and c perfringens adhered to the e tenella-infected cecal mucosa more than to the uninfected cecal mucosa, but fewer bacteroides vulgatus and bifidobacterium thermophilum adhered to the e tenella-infected ceca than to the uninfected ceca. many bacteria ...19921575383
population of salmonella serovar typhimurium in the cecum of gnotobiotic chickens with escherichia coli and intestinal bacteria.to test the interaction between various species of bacteria and salmonella serovar typhimurium (s. typhimurium), the population of s. typhimurium was measured in the cecum of gnotobiotic chickens in the presence of escherichia coli (e. coli) and one of the four intestinal bacteria; lactobacillus acidophilus. clostridium perfringens, bifidobacterium thermophilum and bacteroides vulgatus. competitive exclusion of s. typhimurium by di-flora chicken was not demonstrated. but the population of s. typ ...19911830793
influence of whey and probiotic-supplemented withdrawal feed on the retention of salmonella intubated into market age broilers.broilers (6 wk of age) were crop-intubated with a nalidixic acid-resistant strain of salmonella typhimurium and given four withdrawal feeds: 1) control feed (corn and soybean meal base, 18% cp and 3,200 kcal me/kg); 2) control plus 5% whey (61% lactose); 3) control plus probiotic (6.8 x 10(6) cfu of bifidobacterium pseudolongum, bifidobacterium thermophilium, and lactobacillus acidophilus); and 4) control plus 5% whey plus probiotic. in experiment 1, male broilers received 9.8 x 10(7) cfu of s. ...19902124688
population of salmonella typhimurium in the cecum of gnotobiotic chickens.to test the interaction of various species of bacteria with salmonella typhimurium, levels of s. typhimurium were measured in the cecum of gnotobiotic chickens inoculated with various test bacteria and subsequently with s. typhimurium. the population of s. typhimurium was suppressed in the cecum of chickens previously inoculated with escherichia coli. populations of bacteroides vulgatus, clostridium perfringens, bifidobacterium thermophilum, or lactobacillus acidophilus were transiently, though ...19892539590
electron microscopic, biochemical and physiological studies of bifidobacterium pseudolongum ss-24 and bifidobacterium thermophilum ss-19.comparative studies of physiology, biochemical characteristics, and morphology by electron microscopy were conducted on bifidobacterium pseudolongum ss-24 isolated from dogs and bifidobacterium thermophilum ss-19 isolated from swine. both b. pseudolongum and b. thermophilum grow unusually rapidly in the rumen fluid medium of scott and dehority, and reached a maximum of optical density after only 6 to 7 h of incubation. b. pseudolongum and b. thermophilum showed similar patterns of results for 21 ...19892803454
effect of infection with eimeria tenella upon the cecal bacterial population in monoflora chickens.monoflora chickens were established at 2 days of age by an oral inoculation with one of six species of bacteria (lactobacillus acidophilus, bifidobacterium thermophilum, bacteroides vulgatus, clostridium perfringens, escherichia coli, or streptococcus faecalis) and were infected with eimeria tenella (5 x 10(4) oocysts per bird) 2 days later. there were two experimental groups for each bacterium: birds infected with bacteria alone and birds infected with a combination of bacteria and e. tenella. ...19872888103
the influence of various bacteria on the cecal mucosa of monoflora chickens infected with eimeria tenella. a scanning electron microscopic study.monoflora chickens were established at the age of 2 days by an oral inoculation of one of six species of bacteria (lactobacillus acidophilus, bifidobacterium thermophilum, bacteroides vulgatus, clostridium perfringens, escherichia coli, or streptococcus faecalis). two days later the chickens were infected with eimeria tenella (5 x 10(4) oocytes per bird). there were four groups: uninfected birds as controls, the birds infected with either bacteria or e. tenella alone, and the birds infected with ...19873673342
[comparative studies on bifidobacteria isolated from the alimentary tract of man and animals (including descriptions of bifidobacterium thermophilum nov. spec. and bifidobacterium pseudolongum nov. spec)]. 19695361078
iron uptake by bifidobacterium thermophilum protoplasts.protoplasts of bifidobacterium thermophilum were prepared by a combination of lysozyme and protease digestion, and ferrous iron uptake studies were carried out. little, if any, iron was internalized by the protoplasts, although large amounts of iron were bound to the protoplast surface. this binding was much greater than that of intact cells, which prefer to internalize iron by an energy-dependent process. it was also found that the binding of iron by protoplasts of cells grown in an iron-defici ...19937691127
enhancement of cytotoxic activity of lymphocytes in mice by oral administration of peptidoglycan (pg) derived from bifidobacterium thermophilum.a preparation of peptidoglycan (pg) of bifidobacterium thermophilum (b. thermophilum) of swine was orally administered to spf-c57bl/6crslc mice in order to confirm the enhancement of the cytotoxic activity of natural killer cells (nk), intraperitoneal cytotoxic t lymphocytes (ctl) and lymphocytes stimulated by concanavalin a (con a-stimulated lymphocytes). the nk cells from the spleen and the mesenteric lymph node (mln) of mice that were continuously fed with pg-mixed feed for three weeks showed ...19947696404
enhanced resistance of mice to escherichia coli infection induced by administration of peptidoglycan derived from bifidobacterium thermophilum.peptidoglycan (pg) of bifidobacterium thermophilum (b. thermophilum) from swine were orally administered to spf-icr mice in order to confirm the enhancement of the defence activity of the mice against escherichia coli (e. coli) infection. it was found that the survival rates of the pg-administered group were significantly higher than those of the non-treated control group after the single oral administration of pg. and the proper concentration at which pg enhanced defence activity most effective ...19947948368
increase of mannose residues, as salmonella typhimurium-adhering factor, on the cecal mucosa of germ-free chickens infected with eimeria tenella.to study increase of the salmonella population in the cecum of chickens infected with eimeria tenella, quantitative changes in mannose residues on the cecal mucosa were investigated. inhibition of s typhimurium adherence to the cecum by a 2% carbohydrate (d-mannose, d-galactose, l-fucose, alpha-methyl-d-glucoside) in phosphate-buffered saline solution was examined. only d-mannose had inhibitory effects. whereas, d-galactose had somewhat enhancing effects on adherence of s typhimurium to the ceca ...19938239135
effects of mg2+ and ca2+ on fe2+ uptake by bifidobacterium thermophilum.ferrous iron uptake was investigated in bifidobacterium thermophilum (b. thermophilum) in the presence of mg2+ and ca2+ with the following findings: 1. mg2+ inhibited fe2+ accumulation in the cells in a dose-dependent manner at 37 degrees, but not at 0 degrees. removal of mg2+ from the medium resulted in a resumption of rapid iron uptake. 2. mg2+ had no effect on the binding of fe2+ by b. thermophilum protoplasts, its cellular particulate fraction, or distribution between the particulate and sol ...19938365544
phagocytosis of splenetic neutrophils of mice enhanced by orally administered peptidoglycan from bifidobacterium thermophilum.a preparation of peptidoglycan (pg) of swine bifidobacterium thermophilum was orally administered to spf-balb/c and icr mice and its effect on phagocytosis splenetic neutrophils from pg administered mice was measured by chemiluminescent response (cl) and fluorometric analysis and the result was compared with that of non-treated mice. pg stimulated phagocytosis of neutrophils in a dose-dependent manner, whereas dosage exceeding the optimum concentration (500 microgram) inhibited phagocytosis. the ...19968645766
digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples.the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ...19979241587
binding of ferric iron to the cell walls and membranes of bifidobacterium thermophilum: effect of free radicals.bifidobacterium thermophilum (atcc 25866) was incubated with 100-120 microm (59)feso(4) and 300 microm excess of h(2)o(2) for up to 120 min in the presence and absence of glucose. samples were withdrawn after 5, 30, 60, and 120 min. these were protoplasted and (59)fe(iii) measured in the supernatant fraction (cell walls) and protoplasts (cell membranes). these experiments were also repeated in the presence of 400 microm al(iii), which, in whole cells, caused an increase in fe(iii) binding. the a ...199910552858
adsorption effect of activated charcoal on enterohemorrhagic escherichia coli.the adsorption property of activated charcoal on verotoxin (vt)-producing escherichia coli (vtec) was examined using e. coli o157:h7. in the present study, e. coli o157:h7 strains were effectively adsorbed by activated charcoal. adsorption was dose-dependent, and the maximum adsorption occurred within 5 min. at 10 mg of activated charcoal, bacteria tested were completely adsorbed. activated charcoal also had the capacity to adsorb toxin (verotoxin 2) activity from the bacterial extract. furtherm ...200111307928
the effect of nisin on the physiology of bifidobacterium thermophilum.the effects of nisin on lactate accumulation, growth, and fe(iii) binding by bifidobacterium thermophilum (atcc 25866) and bifidobacterium breve (atcc 15700) were investigated. nisin inhibited lactate production by b. thermophilum at concentrations of less than 1 microg/ml, but this effect could be largely eliminated by pretreatment of the organism with 100 to 400 microm al(iii) or la(iii). nisin also inhibited the growth of b. thermophilum at concentrations of 2 to 3 microg/ml, with lower conce ...200111510661
effect of al(iii) on surface properties of bifidobacterium thermophilum as a function of temperature.the effects of al(iii) on surface properties and lactate accumulation by bifidobacterium thermophilum were investigated. bacteria were treated with al(iii) at 37 degrees c and 4 degrees c, then exposed to free radicals or nisin. when exposed to al(iii) at 37 degrees c, the organism exhibited spreading on hydrophobic surfaces and showed high susceptibility to free-radical alteration as indicated by fe(iii) binding, but showed little effect on lactate production in the presence or absence of nisin ...200212008978
enhancement of chicken resistance against escherichia coli infection by oral administration of bifidobacterium thermophilum preparations.three types of bifidobacterium thermophilum extract were prepared and fed to 2-wk-old chickens to evaluate their usefulness in enhancing the defense activity of the chickens against pathogenic escherichia coli. all three preparations resulted in significant reduction (p < 0.05) of e. coli numbers in the lungs of the treated chicken groups compared with the control nontreated group. besides, improvement in the survival rate was observed in the treated chicken groups, especially the one administer ...200212243516
species identification of genus bifidobacterium based on partial hsp60 gene sequences and proposal of bifidobacterium thermacidophilum subsp. porcinum subsp. nov.sequence homology of partial 60 kda heat-shock protein (hsp60) genes was analysed for 50 bifidobacterium strains that represent 12 bifidobacterium species and subspecies with validly published names. sequence similarities were 96.5-100 % within the same species, 95.5-97 % at the subspecies level and 80-96 % (mean, 88 %) at the interspecies level among the 10 bifidobacterium species. hence, the hsp60 gene was a more accurate tool for species identification within the genus bifidobacterium than 16 ...200313130059
eliminating the carriage of salmonella enterica serovar enteritidis in domestic fowls by feeding activated charcoal from bark containing wood vinegar liquid (nekka-rich).the protective efficacy of activated charcoal containing wood vinegar liquid (nekka-rich) against intestinal infection with salmonella enterica serovar enteritidis was sought. in the present study, the adsorption effects of activated charcoal of nekka-rich on s. enteritidis and normal bacterial flora in the intestine, enterococcus faecium, were examined. s. enteritidis was effectively adsorbed by activated charcoal of nekka-rich. on the other hand, activated charcoal of nekka-rich showed lower b ...200515844805
bifidobacterium species isolated from animal feces and from beef and pork meat.bifidobacteria were isolated from 122 of 145 samples of animal feces (from cattle, swine, sheep, goats, horses, rabbits, chickens, geese, and pigeons) from farms in france and austria and from 92 of 955 production and processing chain samples of beef and pork (obtained at slaughter, cutting, and retail). bacterial strains were identified to species by phenotypic numerical classification based on api 50ch and id 32a tests and dna-dna hybridization. bifidobacterium pseudolongum was present in 81% ...200616629032
antibiotic susceptibility of bifidobacterium thermophilum and bifidobacterium pseudolongum isolates from animal sources.the widespread use of antimicrobial substances has led to resistant populations of microorganisms in several ecosystems. in animal husbandry, the application of antibiotics has contributed to resistance development in pathogenic and commensal bacteria. these strains or their resistance genes can be spread along several ecological routes, including the food chain. antibiotic resistance is important in terms of the safety of industrial strains, such as probiotics for food and feed. bifidobacterium ...200717265870
class i/class iia bacteriocin cross-resistance phenomenon in listeria monocytogenes.variants resistant to nisin a (va), nisin z (vz), pediocin pa-1 (vp), divergicin m35 (vd) and to bacteriocin-like compounds produced by bifidobacterium thermophilum subsp. infantis rbl67 (vb) were developed from listeria monocytogenes lsd530. lactic acid production, specific growth rate, potassium ion efflux, susceptibility to 13 antibiotics, cell-envelope fatty acid composition and bacteriocin cross-resistance were evaluated. lactic acid production decreased to 75% or less of that by strain lsd ...200717613369
classification of a moderately oxygen-tolerant isolate from baby faeces as bifidobacterium thermophilum.bifidobacteria are found at varying prevalence in human microbiota and seem to play an important role in the human gastrointestinal tract (git). bifidobacteria are highly adapted to the human git which is reflected in the genome sequence of a bifidobacterim longum isolate. the competitiveness against other bacteria is not fully understood yet but may be related to the production of antimicrobial compounds such as bacteriocins. in a previous study, 34 bifidobacterium isolates have been isolated f ...200717711586
detection of the pediocin gene peda in strains from human faeces by real-time pcr and characterization of pediococcus acidilactici uva1.bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures. among them, strains of the genus pediococcus are particularly interesting for their ability to produce pediocin, a broad spectrum antimicrobial peptide with a strong antagonistic activity against the food-borne pathogen listeria monocytogenes. furthermore, there is increasing interest in isolating new bacteriocin-producing strains of human intestinal origin that could be developed for probiotic effects a ...200717850651
antibiotic susceptibility testing of bifidobacterium thermophilum and bifidobacterium pseudolongum strains: broth microdilution vs. agar disc diffusion assay.there is urgent need for having available suitable methods and data regarding the susceptibility levels of antibiotic resistant and sensitive strains of bifidobacteria. based on a defined standard operation procedure, agar disc diffusion and broth microdilution were compared in order to evaluate the antimicrobial susceptibility profiles of 82 b. pseudolongum and 80 b. thermophilum strains mainly originating from the meat production chain. the methods that were assessed showed interpretable agree ...200717884216
hydrogen production by anaerobic microbial communities exposed to repeated heat treatments.biological hydrogen production by anaerobic mixed communities was studied in laboratory-scale bioreactors using sucrose as the substrate. a bioreactor in which a fraction of the return sludge was exposed to repeated heat treatments performed better than a control bioreactor without repeated heat treatment of return sludge and produced a yield of 2.15 moles of hydrogen per mole of sucrose, with 50% hydrogen in the biogas. terminal restriction fragment length polymorphism analysis showed that two ...200717910366
mosaic tetracycline resistance genes and their flanking regions in bifidobacterium thermophilum and lactobacillus johnsonii.for the first time, mosaic tetracycline resistance genes were identified in lactobacillus johnsonii and in bifidobacterium thermophilum strains. the l. johnsonii strain investigated contains a complex hybrid gene, tet(o/w/32/o/w/o), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(w/32/o) and tet(o/w). as reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for re ...200817967912
resistance determinant erm(x) is borne by transposon tn5432 in bifidobacterium thermophilum and bifidobacterium animalis subsp. lactis.the erm(x) gene from erythromycin- and clindamycin-resistant bifidobacterium strains was characterised by polymerase chain reaction and sequence analysis, including flanking regions. results suggest that the resistance determinant was part of transposon tn5432 that has been described in several opportunistic pathogens such as corynebacterium striatum and propionibacterium acnes.200818378122
pcr and real-time pcr primers developed for detection and identification of bifidobacterium thermophilum in faeces.culture-independent methods based on the 16s ribosomal rna molecule are nowadays widely used for assessment of the composition of the intestinal microbiota, in relation to host health or probiotic efficacy. because bifidobacterium thermophilum was only recently isolated from human faeces until now, no specific real-time pcr (qpcr) assay has been developed for detection of this species as component of the bifidobacterial community of the human intestinal flora.200818847469
co-cultivation of a bacteriocin-producing mixed culture of bifidobacterium thermophilum rbl67 and pediococcus acidilactici uva1 isolated from baby faeces.we investigated the kinetics of growth, metabolism and antimicrobial activity of bifidobacterium thermophilum rbl67 and pediococcus acidilactici uva1 previously isolated as a consortium from human baby faeces and producing antimicrobial proteinaceous compounds.200919298509
identification and antimicrobial susceptibility of porcine bacteria that inhibit the growth of brachyspira hyodysenteriae in vitro.to identify bacilli, lactic acid bacteria and bifidobacteria that inhibit the growth of brachyspira hyodysenteriae.201019778354
in vitro antagonistic activities of animal intestinal strains against swine-associated pathogens.a wide range of enteropathogens cause costly diarrhoeal diseases in fattening piglets and account for food-related infections in humans. the objective of this study was to screen beneficial bacterial strains from the gastrointestinal tract of various animal sources for antagonistic activity against diverse pathogens associated with hazardous pig production times. using agar spot assays, 15 well-characterized strains belonging to lactobacillus, enterococcus, bifidobacterium and bacillus were stud ...201020226602
unexpected consequences of administering bacteriocinogenic probiotic strains for salmonella populations, revealed by an in vitro colonic model of the child gut.new biological strategies for the treatment of salmonella infection are needed in response to the increase in antibiotic-resistant strains. escherichia coli l1000 and bifidobacterium thermophilum rbl67 were previously shown to produce antimicrobial proteinaceous compounds (microcin b17 and thermophilicin b67, respectively) active in vitro against a panel of salmonella strains recently isolated from clinical cases in switzerland. in this study, two three-stage intestinal continuous fermentation m ...201020688827
the insect peptide, coprisin, prevents clostridium difficile-mediated acute inflammation and mucosal damage through selective antimicrobial activity.clostridium difficile-associated diarrhea and pseudomembranous colitis are typically treated with vancomycin or metronidazole, but recent increases in relapse incidence and the appearance of drug-resistant strains of c. difficile indicate the need for new antibiotics. we previously isolated an antibacterial peptide from copris tripartitus, a korean dung beetle, and identified a nine-amino-acid peptide (coprisin: llcialrkk) that had antimicrobial activity. here, we examined whether coprisin treat ...201121807975
Protective effect of probiotics on Salmonella infectivity assessed with combined in vitro gut fermentation-cellular models.ABSTRACT: BACKGROUND: Accurate assessment of probiotics with targeted anti-Salmonella activity requires suitable models accounting for both, microbe-microbe and host-microbe interactions in gut environments. Here we report the combination of two original in vitro intestinal models closely mimicking the complex in vivo conditions of the large intestine. Effluents from continuous in vitro three-stage fermentation colonic models of Salmonella Typhimurium infection inoculated with immobilized child ...201122171685
glycan cross-feeding activities between bifidobacteria under in vitro conditions.bifidobacteria colonize the gut of various mammals, including humans, where they may metabolize complex, diet-, and host-derived carbohydrates. the glycan-associated metabolic features encoded by bifidobacteria are believed to be strongly influenced by cross-feeding activities due to the co-existence of strains with different glycan-degrading properties. in this study, we observed an enhanced growth yield of bifidobacterium bifidum prl2010 when co-cultivated with bifidobacterium breve 12l, bifid ...201526441950
study of the ability of bifidobacteria of human origin to prevent and treat rotavirus infection using colonic cell and mouse models.rotavirus is the leading cause of severe acute gastroenteritis among children worldwide. despite effective vaccines, inexpensive alternatives such as probiotics are needed. the aim of this study was to assess the ability of probiotic candidate bifidobacterium thermophilum rbl67 to inhibit rotavirus infection. bacterial adhesion to intestinal cells and interference with viral attachment were evaluated in vitro. b. thermophilum rbl67 displayed adhesion indexes of 625 ± 84 and 1958 ± 318 on caco-2 ...201627727323
bifidobacterium thermophilum rbl67 impacts on growth and virulence gene expression of salmonella enterica subsp. enterica serovar typhimurium.bifidobacterium thermophilum rbl67 (rbl67), a human fecal isolate and health promoting candidate shows antagonistic and protective effects against salmonella and listeria spec. in vitro. however, the underlying mechanisms fostering these effects remain unknown. in this study, the interactions of rbl67 and salmonella enterica subsp. enterica serovar typhimurium n-15 (n-15) were explored by global transcriptional analysis.201626988691
effect of bifidobacterium thermophilum rbl67 and fructo-oligosaccharides on the gut microbiota in göttingen minipigs.modulating the gut microbiota via dietary interventions is a common strategy to enhance the natural defence mechanisms of the host. several in vitro studies have highlighted the probiotic potential of bifidobacterium thermophilum rbl67 (rbl67) selected for its anti-salmonella effects. the present study aimed to investigate the impact of rbl67 alone and combined with fructo-oligosaccharides (fos) on the gut microbiota of göttingen minipigs. minipigs were fed a basal diet supplemented with 8 g/d p ...201526313935
synergistic effects of bifidobacterium thermophilum rbl67 and selected prebiotics on inhibition of salmonella colonization in the swine proximal colon polyferms model.probiotics and prebiotics are promising strategies to counteract salmonella prevalence in swine. in the present study, we investigated the effects of prebiotics (fructo- (fos), galacto- (gos) and mannan- (mos) oligosaccharides) and the bacteriocinogenic bifidobacterium thermophilum rbl67 (rbl67) on salmonella enterica subsp. enterica serovar typhimurium n-15 (n-15) colonization using the polyferms in vitro continuous fermentation model simulating the swine proximal colon.201425364390
complete genome sequence of the probiotic bifidobacterium thermophilum strain rbl67.bifidobacterium thermophilum rbl67, an isolate from infant feces, exhibits bacteriocin-like antimicrobial activity against listeria spp. and salmonella spp. and protects ht29-mtx cells against salmonella infection. here, the complete genome sequence of the probiotic b. thermophilum strain rbl67 is presented.201323640377
growth-promoting effects of caseinomacropeptide from cow and goat milk on probiotics.caseinomacropeptide (cmp), a 7-kda phosphoglycopolypeptide fragment released from κ-casein during milk renneting, is heterogeneous with respect to post-translational glycosylation. several studies have reported that cmp has growth-promoting activity on lactic acid bacteria belonging to the genera bifidobacterium. the aim of this study was to evaluate the effect of glycosylation and sequence variations between bovine and caprine cmp on the growth of two probiotics: lactobacillus rhamnosus rw-9595 ...201323182022
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