| production of deoxyfrenolicin and a new antibiotic, frenolicin b by streptomyces roseofulvus strain am-3867. | two antibiotics of frenolicin group, antibiotic am-3867 i and ii were isolated from the fermentation broth of streptomyces roseofulvus strain no. am-3867, a soil isolate. the former was a new antibiotic designated as frenolicin b and its structure containing gamma-lactone was determined, while the latter was identified as deoxyfrenolicin having been chemically prepared from frenolicin. | 1978 | 711620 |
| studies on the antibiosis of streptomyces roseofulvus 210 against bacillus mycoides infection of sugar beet. | streptomyces roseofulvus 210 isolated from the rhizosphere of sugar beet demonstrated antibiotic properties against bacillus mycoides. antibiotic produced was of macrolide character, it was able to inhibit the growth of bacillus mycoides in the soil and to reduce the infection of sugar beet seedlings in micropot and pot experiments. | 1984 | 6083710 |
| [fructose assimilation by differentiated and undifferentiated variants of streptomyces roseofulvus]. | the difference in the growth on fructose between differentiated and adifferentiated variants of streptomyces roseoflavus var. roseofungini should be attributed to their different capacity in forming the enzyme system for fructose uptake whose action is induced by this carbohydrate. in the cells of the two variants grown on glucose and mannitol, the process of induction occurs in the presence of chloramphenicol. in the cells grown on glycerol, chloramphenicol inhibits the induction. the growth of ... | 1980 | 6777647 |
| dna sequence and functions of the actvi region of the actinorhodin biosynthetic gene cluster of streptomyces coelicolor a3(2). | six open reading frames (orfs) were identified by dna sequencing of 5.7 kilobase pairs at the left end of the act cluster (the so-called "actvi region"), in the order: orfb, orfa, orf1, orf2, orf3, orf4. orf1-4 are transcribed rightward and in the same direction as the orfs of the actva region which lies to the right of the actvi region, whereas orfa and orfb run in the opposite direction. by complementation of mutants and gene disruption of the wild type strain, the two previously genetically c ... | 1994 | 7929165 |
| engineered biosynthesis of novel polyketides: influence of a downstream enzyme on the catalytic specificity of a minimal aromatic polyketide synthase. | to identify the minimum set of polyketide synthase (pks) components required for in vivo biosynthesis of aromatic polyketides, combinations of genes encoding subunits of three different aromatic pkss--act from streptomyces coelicolor a3(2) (an actinorhodin producer), fren from streptomyces roseofulvus (a frenolicin and nanaomycin producer), and tcm from streptomyces glaucescens (a tetracenomycin producer)--were expressed in a recently developed streptomyces host-vector system. the "minimal" comp ... | 1994 | 7972098 |
| cloning, sequencing and deduced functions of a cluster of streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin. | a 10.2-kb fragment of dna from streptomyces roseofulvus, which contains polyketide synthase (pks)-encoding genes (fren) presumed to determine production of the antibiotics frenolicin and the nanaomycins, was cloned. a 5530-bp continuous segment of this dna was sequenced. analysis of the sequence revealed five complete open reading frames (orfs) transcribed in one direction (orfs 1, 2, 3, 5, 4) and one (orfx), located between orf3 and orf5, transcribed in the opposite direction. the deduced amino ... | 1994 | 8181754 |
| the oligopeptide permease (opp) of the plant pathogen xanthomonas axonopodis pv. citri. | the oligopeptide permease (opp), a protein-dependent abc transporter, has been found in the genome of xanthomonas axonopodis pv. citri ( xac), but not in xanthomonas campestris pv. campestris ( xcc). sequence analysis indicated that 4 opp genes ( oppa, oppb, oppc, oppd/f), located in a 33.8-kbp dna fragment present only in the xac genome, are arranged in an operon-like structure and share highest sequence similarities with streptomyces roseofulvus orthologs. nonetheless, analyses of the gc conte ... | 2004 | 15060731 |
| a novel hedgehog inhibitor ig2 suppresses tumorigenesis by impairing self-renewal in human bladder cancer. | tumor recurrence is still a major challenge for clinical treatment of bladder cancer. cumulative evidences indicate cancer stem cells (cscs) contribute to drug resistance and leave a putative source for disease relapse. identifying novel agents targeting cscs may represent a new paradigm in the therapy of bladder cancer. here, we separated a novel hedgehog (hh) inhibitor, ig2, from streptomyces roseofulvus, which dramatically blocked the activation of gli2 in bladder cancer cells. the ig2 strong ... | 2016 | 27465044 |