human infections caused by brevibacterium casei, formerly cdc groups b-1 and b-3.forty-one clinical strains of cdc coryneform groups b-1 and b-3 were compared biochemically, by analysis of cell wall sugars, amino acids, and cellular fatty acids, and by dna relatedness to the type strains of brevibacterium casei, brevibacterium epidermidis, and brevibacterium linens. twenty-two strains were shown to be b. casei, while five other strains formed a phenotypically inseparable genomospecies in the same genus. the remaining isolates were genetically heterogeneous, and most are prob ...19948077397
recurrent bacteremia due to brevibacterium casei in an immunocompromised patient.a case of an immunocompromised patient who experienced two episodes of septicemia caused by a coryneform bacterium is reported. biochemical characteristics and analysis of cellular fatty acids and of cell wall components showed two identical strains of brevibacterium casei to be responsible for these infections. the lack of easy-to-perform methods for identification may have led, in the past, to an underestimation of the role of this bacterium, especially in immunocompromised patients.19958681984
antimicrobial susceptibility patterns of some recently established coryneform bacteria.the susceptibility patterns of 480 isolates representing six recently defined species of coryneform bacteria (corynebacterium amycolatum [n = 101], corynebacterium auris [n = 48], corynebacterium glucuronolyticum [n = 86], brevibacterium casei [n = 50], dermabacter hominis [n = 49], and turicella otitidis [n = 146]) to 17 antimicrobial agents were determined by an agar dilution method. most significantly, for c. amycolatum strains the mics at which 90% of isolates are inhibited were > or = 32 mi ...19969124857
broviac catheter-related bacteraemias due to unusual pathogens in children with cancer: case reports with literature review.among 102 episodes of intravenous catheter related bacteraemias documented between january 1989 and july 1996 in children receiving antineoplastic chemotherapy or bone marrow transplantation at g. gaslini children's hospital, genoa, italy, were identified seven episodes due to unusual pathogens: bacillus circulans, bacillus licheniformis, brevibacterium casei, flavimonas oryzihabitans, porphyromonas asaccharolytica, comamonas acidovorans and agrobacterium radiobacter. susceptibility to different ...19979200028
brevibacterium casei sepsis in an 18-year-old female with aids.brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with aids. the isolate was identified as brevibacterium casei by use of carbohydrate assimilation tests. treatment was successful with intravenously administered ciprofloxacin. to our knowledge, this is the first report of sepsis caused by b. casei in a human immunodeficiency virus-infected patient.200010970420
microorganism selection and biosurfactant production in a continuously and periodically operated bioslurry reactor.a continuous-flow reactor (cstr) and a soil slurry-sequencing batch reactor (ss-sbr) were maintained in 8l vessels for 180 days to treat a soil contaminated with diesel fuel (df). concentrations of candida tropicalis, brevibacterium casei, flavobacterium aquatile, pseudomonas aeruginosa, and pseudomonas fluorescens were determined using fatty acid methyl ester (fame) analysis. df removal (biological and volatile) and biosurfactant concentrations were measured. the ss-sbr encouraged the growth of ...200111406310
natural antibiotic susceptibility of recently established coryneform bacteria.the natural susceptibility of 20 strains each of brevibacterium casei (formerly cdc coryneform groups b-1 and b-3), dermabacter hominis (formerly cdc coryneform groups 3 and 5), and turicella otitidis (formerly coryneform group anf-1-like) isolated from clinical specimens to 71 antibiotics was investigated. susceptibility testing was carried out with a microdilution procedure using h medium. all three species were naturally sensitive to tetracyclines, most aminoglycosides, carbapenems, macrolide ...200111453591
brevibacterium casei bacteremia and line sepsis in a patient with aids.brevibacteria are obligately aerobic gram-positive bacilli that are associated with milk products and are also found on human skin. strains of brevibacterium casei have been found to correspond to centers for disease control coryneform groups b-1 and b-3 and have been isolated from a variety of human clinical specimens. in this report, we describe a case of b. casei bacteremia and sepsis in a patient with aids associated with a contaminated hickmann catheter and review the microbiology and chara ...200312504612
management of long-term catheter-related brevibacterium bacteraemia.brevibacterium has been reported as a rare cause of implanted-device infection. in two cases of recurrent brevibacterium casei bacteraemia associated with infection of surgically implanted intravascular devices, relapse occurred 2 and 5 months, respectively, after completion of therapy with vancomycin via the infected catheter. a second intravenous antibiotic therapy course by the antibiotic-lock technique led to bacteriological cure in one patient. molecular typing results demonstrated that the ...200415113328
identification of a novel brevibacterium species isolated from humans and description of brevibacterium sanguinis sp. nov.six coryneforms isolated from blood and dialysate fluid were phenotypically similar to brevibacterium casei, but 16s rrna gene sequencing and dna-dna hybridization indicate that they belong to a new species for which the name brevibacterium sanguinis is proposed.200415184484
molecular detection and isolation of facultatively methylotrophic bacteria, including methylobacterium podarium sp. nov., from the human foot microflora.this is the first study to demonstrate that diverse methylotrophic bacteria occur in the human foot microflora. polymerase chain reaction (pcr) amplification of dna from the soles and toe clefts of feet of five subjects indicated methylobacterium strains to be present in all cases. polymerase chain reaction amplification also showed the gene for the alpha-subunit of methanol dehydrogenase (mxaf) to be present in all samples. two types of mxaf were recovered, one closest to that of methylobacteri ...200415250884
brevibacterium picturae sp. nov., isolated from a damaged mural painting at the saint-catherine chapel (castle herberstein, austria).three strains showing highly similar (gtg)5-pcr patterns were isolated from a heavily damaged mural painting at the saint-catherine chapel (castle herberstein, austria). on the basis of 16s rrna gene sequence similarity, the strains were attributed to brevibacterium, with brevibacterium casei (96.7 %), brevibacterium iodinum (96.7 %) and brevibacterium linens (96.6 %) as the closest related species. chemotaxonomic data [peptidoglycan contains meso-diaminopimelic acid; mycolic acids absent; mk-8( ...200415388707
brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga.two whitish yellow, gram-positive, non-motile, aerobic bacteria were isolated from enrichment culture during degradation of the thallus of the brown alga fucus evanescens. the bacteria studied were chemo-organotrophic, mesophilic and grew well on nutrient media containing up to 15 % (w/v) nacl. the dna g+c content was 61 mol%. the two isolates exhibited a conspecific dna-dna relatedness value of 98 %, indicating that they belong to the same species. a comparative analysis of 16s rrna gene sequen ...200415545442
pericardial infection caused by brevibacterium casei. 200515679496
isolation and molecular detection of methylotrophic bacteria occurring in the human mouth.diverse methylotrophic bacteria were isolated from the tongue, and supra- and subgingival plaque in the mouths of volunteers and patients with periodontitis. one-carbon compounds such as dimethylsulfide in the mouth are likely to be used as growth substrates for these organisms. methylotrophic strains of bacillus, brevibacterium casei, hyphomicrobium sulfonivorans, methylobacterium, micrococcus luteus and variovorax paradoxus were characterized physiologically and by their 16s rrna gene sequence ...200516011760
efficient utilization of ectoine by halophilic brevibacterium species and escherichia coli subjected to osmotic downshock.halophilic and non-halophilic bacteria subjected to osmotic downshock, from 0.7 m nacl to deionized water, were examined for their survival, with the uptake and utilization of the cyclic amino acid ectoine, one of the representative compatible solutes, being taken into account. the uptake of ectoine added externally and survival of the cells were monitored as a function of incubation time in the presence and absence of nacl. the halophilic brevibacterium sp. jcm 6894 and b. epidermidis jcm 2593 ...200516233755
molecular identification and differentiation of brevibacterium species and strains.amplified ribosomal dna restriction enzyme analysis (ardra), pulsed field gel electrophoresis (pfge) and ribotyping were used to differentiate among 24 strains of brevibacterium linens, brevibacterium casei and brevibacterium epidermidis obtained from type culture collections or isolated from various smear ripened cheeses. ardra was applied to the 16s rdna. b. linens was shown to be a quite heterogenic group with 2 to at least 4 copies of rrn operons per strain with aberrant nucleotide sequences ...200716624515
central venous catheter infection with brevibacterium sp. in an immunocompetent woman: case report and review of the literature.brevibacterium spp. were considered apathogenic until a few reports of infections in immunocompromised patients were published. herein, we present a case of a catheter-related septicemia with brevibacterium casei in an immunocompetent patient receiving continuous iloprost infusion for pulmonary arterial hypertension and review the clinical presentation of this mainly emerging opportunistic pathogen.200616703303
[a new polymer of 8,9-di-o-glucosylated 2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid from a cell wall of brevibacterium casei aei ac-2114t].a polysaccharide containing the residues of 2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid (kdn) was found in the cell wall of the brevibacterium casei strain aei ac-2114t . the polymer structure was elucidated by analyzing one-dimensional spectra of 1h and 13c nmr and bidimentional experiments 1h/13c-cosy, tocsy, 1h/13c-ghsqc, and 1h/13c-ghmbc. the polymer is built up of the 2--> 4-linked kdn residues substituted by beta-d-glcp residues at 8- and 9-hydroxyls; such a polymer with disubstitu ...200717375662
development of a large scale process for the conversion of polysialogangliosides to monosialotetrahexosylganglioside with a novel strain of brevibacterium casei producing sialidase.a bioconversion process of producing gm1 (monosialotetrahexosylganglioside) on an industrial scale was developed with a novel sialidase-producing strain brevibacterium casei. the sialidase hydrolyzed polysialogangliosides to produce gm1 but did not act on gm1. when brevibacterium casei was cultured in a synthetic medium containing crude pig brain gangliosides (10% w/v) at 30 degrees c for 24 h in a 50 l fermenter, most of the polysialogangliosides were converted to gm1. the content of gm1 was in ...200717415532
in vitro activity of linezolid and 12 other antimicrobials against coryneform compare the in vitro activity of linezolid with 12 other antimicrobials against 190 strains of the coryneform bacteria, including 60 strains of c. amycolatum, 30 of c. striatum, 30 of c. jeikeium, 10 of c. urealyticum, 20 of b. casei, 20 of d. hominis and 20 of t. otitidis.200717475450
corneal ulcers associated with aerosolized crack cocaine use.we report 4 cases of corneal ulcers associated with drug abuse. the pathogenesis of these ulcers and management of these patients are also reviewed.200717721298
[decrease of phosphate concentration in the medium by brevibacterium casei cells].brevibacteria able to decrease phosphate concentration in the medium are of interest for the study of the role of bacteria in the phosphorus cycle and for development of biotechnology of phosphate removal from waste. brevibacterium casei, brevibacterium linens, and brevibacterium epidermidis grown in media with initial phosphorus concentrations of 1-11 mm were shown to decrease its concentration by 90%. the composition of the incubation medium required for b. casei to carry out this process was ...200718297865
novel methylotrophic bacteria isolated from the river thames (london, uk).enrichment and elective culture for methylotrophs from sediment of the river thames in central london yielded a diversity of pure cultures representing several genera of gram-negative and gram-positive bacteria, which were mainly of organisms not generally regarded as typically methylotrophic. substrates leading to successful isolations included methanol, monomethylamine, dimethylamine, trimethylamine, methanesulfonate and dimethylsulfone. several isolates were studied in detail and shown by the ...200818681896
maturing dynamics of surface microflora in fontina pdo cheese studied by culture-dependent and -independent study the evolution of rind microbial communities in fontina pdo cheese.200919054234
real-time nanomechanical and topographical mapping on live bacterial cells-brevibacterium casei under stress due to their exposure to co2+ ions during microbial synthesis of co3o4 nanoparticles.the study of elastic properties of microbial and mammalian cells using atomic force microscopy, with force-sensitivity as high as pico-newtons and spatial resolution of a few nanometers, is proving to be a great tool for the real-time observation of the effects of drugs, biomolecules, metal ions, and nanoparticles on cell physiology in their natural environment. it has been shown that the young's modulus of the cell surfaces is extremely sensitive to the surrounding environment. recently, a broa ...200919438181
purification, immobilization, and characterization of nattokinase on phb this study, nattokinase was purified from bacillus subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (phb) nanoparticles. a novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (pha) and the strain was identified as brevibacterium casei srkp2. pha granules were extracted from 48 h culture and the ft-ir analysis characterized them as phb, a natural biopolymer from b. casei. nanoprecipitation by solvent displacement te ...200919608412
synthesis of phb nanoparticles from optimized medium utilizing dairy industrial waste using brevibacterium casei srkp2: a green chemistry approach.polyhydroxyalkanoates (phas) are natural, biodegradable polymers accumulated by bacteria under nutritional exhausted condition where carbon source is in excess. a gram positive bacterium (designated strain srkp2) that potentially accumulated polyhydroxybutyrate (phb) was isolated from dairy industrial waste. from its morphological and physiological properties and nucleotide sequence of its 16s rrna, it was suggested that strain srkp2 was similar to brevibacterium casei. phas were synthesized fro ...200919700268
biosynthesis of silver and gold nanoparticles using brevibacterium casei.the present study demonstrates an unprecedented green process for the production of spherical-shaped au and ag nanoparticles synthesized and stabilized using a bacterium, brevibacterium casei. aqueous solutions of chloroaurate ions for au and ag(+) ions for silver were treated with b. casei biomass for the formation of au nanoparticles (aunp) and ag nanoparticles (agnp). the nanometallic dispersions were characterized by surface plasmon absorbance measuring at 420 and 540 nm for ag and au nanopa ...201020197229
characterization of heterotrophic bacteria isolated from the biofilm of a kitchen sink.heterotrophic bacteria constituting the biofilm produced in a kitchen sink drain were analyzed, and the biofilm formation abilities and the hydrophobicity of the cell surface layer were measured for the isolates. when the biofilm sample was cultured at 36 degrees c and 25 degrees c for 7 days, there were about 10 times more colonies on oligotrophic r2a agar medium than on eutrophic bhi agar medium. from isolates from the biofilm sample, 13 bacterial species were detected. to examine the biofilm ...201020361519
biofilm disruption potential of a glycolipid biosurfactant from marine brevibacterium casei.the antibiofilm activity of a glycolipid biosurfactant isolated from the marine actinobacterium brevibacterium casei msa19 was evaluated against pathogenic biofilms in vitro. the isolate b. casei msa19 was a potential biosurfactant producer among the 57 stable strains isolated from the marine sponge dendrilla nigra. the biosurfactant production was optimized under submerged fermentation. the purified glycolipid showed a broad spectrum of antimicrobial activity. based on the minimum inhibitory co ...201020528933
synthesis of silver nanoparticles by glycolipid biosurfactant produced from marine brevibacterium casei msa19.the surfactants are emerging as potential nanoparticle stabilizing agents, however, the synthetic surfactants are not economically viable as well as they are not environmentally friendly. therefore, the biosurfactants are emerging as a green alternate for the synthesis and stabilization of nanoparticles. in this report, a glycolipid biosurfactant was produced from sponge-associated marine brevibacterium casei msa19 under solid state fermentation using the agro-industrial and industrial waste as ...201020600381
simultaneous chromate reduction and azo dye decolourization by brevibacterium casei: azo dye as electron donor for chromate reduction.chromate [cr(vi)] and azo dyes are common pollutants which may co-exist in some industrial effluents. hence studies of biological treatment of industrial wastewater should include investigation of the co-removal of these two pollutants. brevibacterium casei, which can reduce cr(vi) in the presence of the azo dye acid orange 7 (ao7) under nutrient-limiting condition, was isolated from a sewage sludge sample of a dyeing factory. response surface methodology, which is commonly used to optimize grow ...201020656406
diversity and phylogeny of plant growth-promoting bacilli from moderately acidic soil.the molecular diversity of aerobic endospore-forming bacteria, typically bacillus and its derived genera, has been investigated in various environments. however, there have been few investigations concerning bacillus in acidic soils. in this study, the genotypic diversity and phylogenetic relationships among plant growth-promoting (pgp) bacilli isolated from the rice rhizosphere growing in acidic soils of kerala (ph varying from 6.3 to 6.8) were investigated. for assessing their biocontrol poten ...201021077114
development of a cell immobilization technique for the conversion of polysialogangliosides to monosialotetrahexosylganglioside.context: monosialotetrahexosylganglioside (gm(1)) prepared from the brain of pig or bovine is an effective clinical drug in the treatment of different nervous system diseases. generally, polysialogangliosides are transformed into gm(1) by enzymic or chemical hydrolysis due to the very poor level of natural gm(1). objective: to continuously obtain gm(1) by cell immobilization in a packed-bed reactor. materials and methods: brevibacterium casei, which is gram-positive bacteria belonging to the ord ...201121500968
brevibacterium casei as a cause of brain abscess in an immunocompetent patient.coryneform bacteria belonging to the genus brevibacterium have emerged as opportunistic pathogens. of the nine known species of brevibacterium isolated from human clinical samples, brevibacterium casei is the most frequently reported species from clinical specimens. we report the first case of b. casei brain abscess in an immunocompetent patient successfully treated by surgery and antimicrobial therapy.201122012007
Biologically synthesized fluorescent CdS NPs encapsulated by PHB.Here an attempt was made to biologically synthesize fluorescent cadmium sulfide nanoparticles and to immobilize the synthesized nanoparticles in PHB nanoparticles. The present study uses Brevibacterium casei SRKP2 as a potential producer for the green synthesis of CdS nanoparticles. Biologically synthesized nanoparticles were characterized and confirmed using electron microscopy and XRD. The size distribution of the nanoparticles was found to be 10-30 nm followed by which the consequence of time ...201122112944
sonication culture improves microbiological diagnosis of modular megaprostheses.modular megaprostheses are known for high infection rates followed by high rates of revisions. microbial biofilms growing adherently on prosthetic surfaces may inhibit the detection of the pathogens causing prosthetic joint infections. we sought to answer the following questions: does sonication culture (sc) improve the microbiological diagnosis of periprosthetic infections of megaprostheses compared to conventional tissue culture (tc)? which pathogens were detected on the surface of megaprosthe ...201627572456
[recurrent endocarditis due to brevibacterium casei: case presentation and a review of the literature]. 201727381296
rhizospheric bacterial strain brevibacterium casei mh8a colonizes plant tissues and enhances cd, zn, cu phytoextraction by white mustard.environmental pollution by heavy metals has become a serious problem in the world. phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. the aim of this study was to determine whether the multiple-tolerant bacterium, brevibacterium casei mh8a isolated from the heavy metal-contaminated rhizosphere soil of sinapis alba l., is able to promote plant growth and enhance cd, zn, and cu uptake by ...201626909087
cloning, expression, and characterization of a novel sialidase from brevibacterium casei.sialidase gene from brevibacterium casei was cloned in pet28a and overexpressed as a histidine-tagged protein in escherichia coli bl21(de3). the histidine-tagged protein was purified from the crude cell extracts of iptg-induced cells using ni-nta agarose chromatography and was characterized. sds-page using the purified sialidase indicated a single band at 116 kda. this sialidase showed maximum activity at a ph of 5.5 and temperature of 37°c. the kinetic parameters km and vmax for the artificial ...201626748450
[screening and functional properties of cholesterol-degrading lactic acid bacteria from jiangshui].we intended to obtain and characterize lactic acid bacteria with high capacity of cholesterol-degrading.201526665597
the first reported catheter-related brevibacterium casei bloodstream infection in a child with acute leukemia and review of the literature.brevibacterium spp. are catalase-positive, non-spore-forming, non motile, aerobic gram-positive rods that were considered apathogenic until a few reports of infections in immunocompromised patients had been published. to the best of our knowledge, this is the first report of b. casei catheter-related bloodstream infection in a child with acute leukemia. we aim to enhance the awareness of pediatric hematology and infectious disease specialists about this pathogen and review of the literature.201525636191
antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine brevibacterium casei msi04 against shrimp pathogenic vibrios.vibrio pathogens are causative agents of mid-culture outbreaks, and early mortality syndrome and secondary aetiology of most dreadful viral outbreaks in shrimp aquaculture. among the pathogenic vibrios group, vibrio alginolyticus and v. harveyi are considered as the most significant ones in the grow-out ponds of giant black tiger shrimp penaeus monodon in india. use of antibiotics was banned in many countries due to the emergence of antibiotic-resistant strains and accumulation of residual antib ...201425115578
brevibacterium casei isolated as a cause of relapsing peritonitis.we report a case of relapsing peritonitis in a 33-year-old woman on automated peritoneal dialysis. end-stage renal disease was secondary to systemic lupus erythematosus complicated with lupus nephritis. the organism isolated was brevibacterium casei that was not readily identified, delaying appropriate management with an extended antibiotic course. definite management of b casei peritonitis was peritoneal dialysis catheter removal.201424648477
post-traumatic endophthalmitis due to brevibacterium casei : a case report.endophthalmitis is a serious post-traumatic ocular complication that can lead to loss of vision. we report a case of acute post-traumatic endophthalmitis following a penetrating injury caused by an unusual organism, brevibacterium casei . the patient was successfully treated with intravitreal antibiotics like ceftazidime and vancomycin, along with topical cefazolin and tobramycin. brevibacterium casei can be added to the list of rare bacteria causing endophthalmitis and should be kept in mind by ...201323483044
isolation and process parameter optimization of brevibacterium casei for simultaneous bioremediation of hexavalent chromium and pentachlorophenol.chromate and pentachlorophenol are major pollutants discharged through tanneries. three bacteria resistant to high cr(6+) and pcp concentrations simultaneously were isolated. the tvs-3 strain was tolerant to highest 850 mg l(-1) cr(6+) and 1000 mg l(-1) pcp concentration and concomitantly reduced 69% cr(6+) and degraded 72% pcp within 168 h at ph 7.5, 35 ± 1°c temperature, was selected and identified as brevibacterium casei. at 168 h of growth, bacterium showed maximum pcp utilization of 720 mg ...201322733606
brevibacterium ammoniilyticum sp. nov., an ammonia-degrading bacterium isolated from sludge of a wastewater treatment plant.a gram-stain-positive, non-motile, chemo-organotrophic, mesophilic, aerobic bacterium, designated a1(t), was isolated from sludge of a wastewater treatment plant. strain a1(t) showed good ability to degrade ammonia and grew well on media amended with methanol and ammonia. strain a1(t) grew with 0-11 % (w/v) nacl, at 20-42 °c, but not <15 or >45 °c and at ph 6-10 (optimum ph 8.0-9.0). the isolate was catalase-positive and oxidase-negative. the dna g+c content was 70.7 mol%. a comparative analysis ...201322729022
relapsing brevibacterium casei peritonitis: value of 16s rrna gene sequencing in accurate species identification. 201522641738
brevibacterium casei sp. nov. and brevibacterium epidermidis sp. nov.dna base compositions, dna/dna homologies and lipid studies were performed on some dairy and skin coryneform bacteria in an attempt to clarify their taxonomy. the results of the present and earlier studies (sharpe et al., 1978) indicate the dairy and skin isolates represent two new species of the genus brevibacterium. the isolates from cheese and milk are named brevibacterium casei sp. nov. and those from human skin brevibacterium epidermidis sp. nov. the type strains are ncdo 2048 (cmd1) and nc ...198323194737
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