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reclassification of brevibacterium imperiale (steinhaus) and "corynebacterium laevaniformans" (dias and bhat) in a redefined genus microbacterium (orla-jensen), as microbacterium imperiale comb. nov. and microbacterium laevaniformans nom. rev.; comb. nov.chemical, morphological and physiological data indicate a close relationship between brevibacterium imperiale (steinhaus), "corynebacterium laevaniforms" (dias and bhat) and the species microbacterium lacticum (orla-jensen). it is suggested that b. imperiale and "c. laevaniformans" be reclassified in a redefined genus microbacterium, as microbacterium imperiale (steinhaus) comb. nov. and microbacterium laevaniformans (dias and bhat) nom. rev.; comb, nov., respectively.198323196300
characterization and mechanism of action of microbacterium imperiale glucan 1,4-α-maltotriohydrolase.in this study, glucan 1,4-α-maltotriohydrolase (amts) from microbacterium imperiale was purified and characterized. hydrolysis by amts was affected by starch structure (e.g., amylose versus amylopectin) and hydrolysis time. during the initial phase of hydrolysis of maltooligosaccharides (g4-g7), amts displayed a unique transfer specificity to the transfer of maltotriosyl units. after extensive hydrolysis, maltotriose became the major end product, followed by glucose and maltose. maltotetraose (g ...201424345719
rhizosphere associated bacteria trigger accumulation of terpenes in leaves of vitis vinifera l. cv. malbec that protect cells against reactive oxygen species.it has been proposed that plant growth promoting rhizobacteria (pgpr) stimulate plant growth and development by inducing the biosynthesis of secondary metabolites, like terpenes, which reduce stress incidence. three bacteria previously isolated from grapevine roots and adjacent soil (microbacterium imperiale rz19m10, kocuria erythromyxa rt5m10 and terribacillus saccharophilus rt17m10) were tested as pgpr. after 30 days since root inoculation of in vitro grown vitis vinifera cv. malbec plants, th ...201627231874
High-yield continuous production of nicotinic acid via nitrile hydratase-amidase cascade reactions using cascade CSMRs.High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase-amidase enzymatic cascade system of Microbacterium imperiale CBS 498-74. Experiments were carried out in continuously stirred tank UF-membrane bioreactors (CSMRs) arranged in series. This reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences. ...201122112948
microbacterium radiodurans sp. nov., a uv radiation-resistant bacterium isolated from soil.strain gimn 1.002(t), a uv radiation-tolerant bacterium, was isolated from the upper sand layers of the gobi desert, xinjiang, china and characterized in order to determine its taxonomic position. cells were gram-reaction-positive, heterotrophic, strictly aerobic, short rods. 16s rrna gene sequence analysis revealed that strain gimn 1.002(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens dsm 20754(t) (98.8 % 16s rrna gene sequence similarity) and micro ...201020061506
microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.strain gsoil 259(t), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain gsoil 259(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens ifo 3750(t) (98.5 %) and microbacterium imperiale ifo 1261 ...200818218944
purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant brachybacterium sp. strain lb25.a bacterium that secretes maltooligosaccharide-forming amylase in a medium containing 12.5% (vol/vol) dimethylsulfoxide (dmso) was isolated and identified as brachybacterium sp. strain lb25. the amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kda. the enzyme was stable at ph 7.0-8.5 and active at ph 6.0-7.5. the optimum temperature at ph 7.0 was 35 degrees c in the presence of 5 mm cacl(2). the enzyme hydrolyzed starch to produce maltotriose primari ...200717619813
microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest.a xylanolytic bacterium, us15t, was isolated from swamp forest soil in ulsan, korea. the cells of the novel strain were gram-positive, non-motile, short-rod-shaped and showed chemotaxonomic properties that were consistent with its classification in the genus microbacterium. chemotaxonomic results showed mk-12 and mk-11 as major menaquinones, predominating iso- and anteiso-branched cellular fatty acids, glucose, galactose and mannose as cell-wall sugars, peptidoglycan-type b2beta with glycolyl re ...200616514023
nitrile bioconversion by microbacterium imperiale cbs 498-74 resting cells in batch and ultrafiltration membrane bioreactors.the biohydration of acrylonitrile, propionitrile and benzonitrile catalysed by the nhase activity contained in resting cells of microbacterium imperiale cbs 498-74 was operated at 5, 10 and 20 degrees c in laboratory-scale batch and membrane bioreactors. the bioreactions were conducted in buffered medium (50 mm na(2)hpo(4)/nah(2)po(4), ph 7.0) in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. the integral bioreactor performances were studied w ...200615739103
microbacterium ulmi sp. nov., a xylanolytic, phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra.a xylanolytic and phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra in salamanca was characterized by a polyphasic approach. the novel strain, designated xil02(t), was gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped and non-motile. phylogenetically and chemotaxonomically, it was related to members of the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium arborescens and microbacterium imperiale; howev ...200415023968
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