Publications

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comparison of maldi tof with conventional identification of clinically relevant bacteria.a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory.201020924806
pathogenicity of bacteria isolated from gut of spodoptera litura (lepidoptera: noctuidae) and fitness costs of insect associated with consumption of bacteria.gut microbes contribute to the health of insects and perturbations in the composition or location of gut microbiota can lead to pathological states and host mortality. we explored the culturable bacterial community in the gut of spodoptera litura (fab.) larvae, which is a polyphagous pest. bacterial isolates were identified as microbacterium arborescens (sl6), enterococcus casseliflavus (sl10) and enterobacter cloacae (sl11) by using culture dependent technique based on 16s rrna gene sequencing. ...201525725116
a novel dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of n-acyl amino acids.a novel type of a microbial n-acyl amino acid hydrolase (aah) from insect gut bacteria was purified, cloned and functionally characterized. the enzyme was obtained from microbacterium arborescens se14 isolated from the foregut of larvae of the generalist herbivore spodoptera exigua. the substrates of aah are n-acyl-glutamines previously reported to elicit plant defence reactions after introduction into the leaf during feeding. the isolated aah catalyses the hydrolysis of the amide bond (k(m) = 3 ...200717504494
coevolution of aah: a dps-like gene with the host bacterium revealed by comparative genomic analysis.a protein named aah was isolated from the bacterium microbacterium arborescens se14, a gut commensal of the lepidopteran larvae. it showed not only a high sequence similarity to dps-like proteins (dna-binding proteins from starved cell) but also reversible hydrolase activity. a comparative genomic analysis was performed to gain more insights into its evolution. the gc profile of the aah gene indicated that it was evolved from a low gc ancestor. its stop codon usage was also different from the ge ...201222454608
microbacterium kribbense sp. nov., isolated from soil.a gram-positive, short, rod-shaped, cream-coloured actinobacterium, designated msl-04(t), was isolated from a soil sample collected from an agricultural field on bigeum island (republic of korea) and was subjected to polyphasic characterization to unravel its taxonomic position. the optimum growth temperature and ph were 28-30 degrees c and ph 7.2-7.4. chemotaxonomic characteristics such as b2beta-type peptidoglycan with glycolyl residues, mk-11 and mk-12 as the major menaquinones and a fatty ac ...200818984689
microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.strain gsoil 259(t), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain gsoil 259(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens ifo 3750(t) (98.5 %) and microbacterium imperiale ifo 1261 ...200818218944
characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis.the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ...200415183874
structure and mechanism of iron translocation by a dps protein from microbacterium arborescens.dps enzymes are a major class of dodecameric proteins which bacteria use to detoxify their cytosol through the uptake of reactive iron species. in the stationary growth phase of bacteria, dps enzymes (dna protection during starvation) are primarily used to protect dna by bio-crystallization. to characterize the wildtype dps protein from microbacterium arborescens, which displays additional catalytic functions (amide hydrolysis and synthesis), we determined the crystal structure to a resolution o ...201121768097
microbacterium radiodurans sp. nov., a uv radiation-resistant bacterium isolated from soil.strain gimn 1.002(t), a uv radiation-tolerant bacterium, was isolated from the upper sand layers of the gobi desert, xinjiang, china and characterized in order to determine its taxonomic position. cells were gram-reaction-positive, heterotrophic, strictly aerobic, short rods. 16s rrna gene sequence analysis revealed that strain gimn 1.002(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens dsm 20754(t) (98.8 % 16s rrna gene sequence similarity) and micro ...201020061506
bioavailability of selenium accumulated by selenite-reducing bacteria.the bioavailability of selenium (se) was determined in bacterial strains that reduce selenite to red elemental se (seo). a laboratory strain of bacillus subtilis and a bacterial rod isolated from soil in the vicinity of the kesterson reservoir, san joaquin valley, ca, (microbacterium arborescens) were cultured in the presence of 1 mm sodium selenite (na2seo3). after harvest, the washed, lyophilized b. subtilis and m. arborescens samples contained 2.62 and 4.23% total se, respectively, which was ...19968811279
sand aggregation by exopolysaccharide-producing microbacterium arborescens--agsb.in the rhizosphere, exopolymers are also known to be useful to improve the moisture-holding capacity. the ability of the isolates from coastal sand dunes to produce exopolymers was determined. among which the isolate, showing very high production of exopolysaccharide (eps), microbacterium arborescens--agsb, a facultative alkalophile was further studied for exopolymer production. the isolate a gram-positive non-spore forming, slender rod, catalase positive, oxidase negative, showed growth in 12% ...200919340484
microbacterium ulmi sp. nov., a xylanolytic, phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra.a xylanolytic and phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra in salamanca was characterized by a polyphasic approach. the novel strain, designated xil02(t), was gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped and non-motile. phylogenetically and chemotaxonomically, it was related to members of the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium arborescens and microbacterium imperiale; howev ...200415023968
[a case of mixed meningitis caused by alcaligenes faecalis and flavobacterium arborescens]. 195313073497
polyethyleneimine-grafted collagen fiber as a carrier for cell immobilization.collagen fiber (cf), an abundant natural biopolymer, features many favorable properties that make it a potential carrier for cell immobilization. in the present investigation, cf was grafted with polyethyleneimine (pei) using glutaraldehyde (ga) as the cross-linking agent, resulting in the formation of a novel cf based carrier (cf-pei). the properties of cf-pei as a carrier were evaluated by the immobilization of microbacterium arborescens (cicc 20196), which has glucose isomerase (ec 5.3.1.5) a ...201525540047
purification and characterization of an extracellular protease from flavobacterium arborescens.an extracellular protease from flavobacterium arborescens has been purified to an apparent homogeneity and characterized. the enzyme is most active at ph 8-10.5, requires no metal cofactor, and is inhibited by diisopropyl fluorophosphate. the protease is nonspecific, is active at temperatures up to 60 degrees c, and is completely free of nucleases. the ease of purification and freedom from nucleolytic contaminants make the protease a useful deproteinizing agent in dna and rna manipulations.19836353994
bacteria isolated from field cases of equine amnionitis and fetal loss.a series of unusual abortions occurred in thoroughbred and quarterhorse mares in the hunter valley region of new south wales from mid-march to november 2004. the initial link between early cases was the microbiological culture of atypical environmental coryneforms from the stomach contents and/or lungs of fetuses aborted on different properties.201323521098
bacterial endophytes enhance phytostabilization in soils contaminated with uranium and lead.the combined use of plants and bacteria is a promising approach for the remediation of polluted soil. in the current study, the potential of bacterial endophytes in partnership with leptochloa fusca (l.) kunth was evaluated for the remediation of uranium (u)- and lead (pb)-contaminated soil. l. fusca was vegetated in contaminated soil and inoculated with three different endophytic bacterial strains, pantoea stewartii asi11, enterobacter sp. hu38, and microbacterium arborescens hu33, individually ...201728324669
microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest.a xylanolytic bacterium, us15t, was isolated from swamp forest soil in ulsan, korea. the cells of the novel strain were gram-positive, non-motile, short-rod-shaped and showed chemotaxonomic properties that were consistent with its classification in the genus microbacterium. chemotaxonomic results showed mk-12 and mk-11 as major menaquinones, predominating iso- and anteiso-branched cellular fatty acids, glucose, galactose and mannose as cell-wall sugars, peptidoglycan-type b2beta with glycolyl re ...200616514023
enhanced degradation of textile effluent in constructed wetland system using typha domingensis and textile effluent-degrading endophytic bacteria.textile effluent is one of the main contributors of water pollution and it adversely affects fauna and flora. constructed wetland is a promising approach to remediate the industrial effluent. the detoxification of industrial effluent in a constructed wetland system may be enhanced by applying beneficial bacteria that are able to degrade contaminants present in industrial effluent. the aim of this study was to evaluate the influence of inoculation of textile effluent-degrading endophytic bacteria ...201424755300
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