the growtn of microbacterium thermosphactum on beef. 197944291
isoprenoid quinones in the classification of coryneform and related bacteria.menaquinones were the only isoprenoid quinones found in 85 of the 95 coryneform bacteria examined. dihydromenaquinones having nine isoprene units were the main components isolated from corynebacterium bovis, from other glutamic acid-producing strains, and from arthrobacter globiformis and related species. dihydromenaquinones with eight isoprene units were found in brevibacterium linens, the remaining corynebacterium species and strains probably belonging to the genus rhodococcus. tetrahydromenaq ...1979107269
energetics of microbacterium thermosphactum in glucose-limited continuous culture.microbacterium thermosphactum was grown at 25 degrees c in glucose-limited continuous culture under aerobic (greater than 120 microm oxygen) and anaerobic (less than 0.2 microm oxygen) conditions. the end products of the anaerobic metabolism of glucose were identified as l-lactate and ethanol. together these compounds accounted for between 85 and 90% of the glucose utilized over the full range of growth rates studied. in addition, 4% of the glucose utilized was incorporated into cellular materia ...1979114113
the microbiology of polony.a survey of 25 polony samples of different brands available to the consumer in bloemfontein showed that significant numbers of various micro-organisms were present. aerobic micro-organisms, psychrophiles, micrococci, pseudomonads, microbacterium thermosphactum, lactobacilli, yeasts and moulds were enumerated by the plate count technique. the mean aerobic plate count was 15,850/g and counts ranged between less than 100/g and greater than 1 million/g. selective plate counts indicated that psychrop ...1978366151
[destruction of microbacterium lacticum, escherichia coli and staphylococcus aureus in milk by spray-drying. i. selective count of surviving bacteria].in this paper a method which allows the measure of microbial death rate during spray-drying by means of a streptomycin-resistant mutant that can be grown on a streptomycin-containing agar is described. plate counts of microbacterium lacticum, escherichia coli, and staphylococcus aureus recovered from skim milk powders were done on plate count agar in the presence and absence of streptomycin and on various selective media. the powders were produced from evaporated milk previously inoculated with ...1977406026
[destruction of microbacterium lacticum, escherichia coli and staphylococcus aureus in milk by sprayedrying. ii. effect of drying conditions].bacterial death during spray drying of skim milk is essentially related to the outlet temperature of the spray drier and the type of bacteria. under industrial spray drying conditions, survival rates of microbacterium lacticum, staphylococcus aureus and escherichia coli were 50, 2, and 0.002%, respectively. these rates may vary by a 10(4) factor for outlet temperatures between 65 and 105 degrees c. no simple mathematical equation could be derived to describe the relation between bacterial death ...1977406027
[preservation of meats in a controlled atmosphere].commercializing meat sets problems more particularly connected with its organoleptic and microbiological qualities. as these qualities, resulting from biochemical and microbial effects, develop during the storage, the conditioning must precisely help their controlling. after examining their factors, the author analyses the processes of conditioning which he classifies in three categories according to the techniques used and the desired finality (need to store at -1 degree c + 3 degrees c). 1. co ...1978707932
effect of lactobacilli and carbon dioxide on the growth of microbacterium thermosphactum on fresh beef.studies with articifially inoculated fresh beef showed that lactobacilli markedlyrestrict the growth of microbacterium thermosphactum when the beef is vacuum-packaged. high concentrations of carbon dioxide (up to 75%) had little effect on the growthof m. thermosphactum under either aerobic or anaerobic conditions. tests were madewith samples of lean beef inoculated with pure cultures of lactobacilli and (or) m. thermosphactum and stored unpackaged in air, in air enriched with carbon dioxide, in ...1975804984
a numerical taxonomic study of coryneform and related bacteria.two hundred and thirty-three strains of coryneform bacteria, including representatives of the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix, jensenia, kurthia, listeria, microbacterium, mycobacterium, nocardia and propionibacterium and other related bacteria, were studied using 173 morphological, physiological and biochemical tests. the bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 degrees c ...1975805825
a numerical taxonomic survey of listeria and related bacteria.a numerical taxonomic study was performed on named strains of listeria, erysipelothrix, microbacterium thermosphactum, lactobacillus, streptococus, propionibacterium, kurthia and some possibly related bacteria using i43 unit characters covering a wide range of properties. the strains fell into six main clusters: (a) listeria; (b) microbacterium thermosphactum, lactobacillus, streptococcus; (c) gemella, erysipelothrix; (d) kurthia and mainly aerobic corynebacteria; (e) propionibacterium, staphylo ...1977856940
[survival of epiphytic microorganisms following sublimation dehydration and storage].epiphytic microorganisms belonging to the genera pseudomonas, agrobacterium, achromobacter, flavobacterium, mycobacterium, mycococcus, nocardia, corynebacterium, arthrobacter, bacillus, and microbacterium were dehydrated by sublimation employing two suspension media, and were stored at 3-5 degrees c during 1-2.5 years. survival of epiphytic microorganisms upon dehydration by sublimation and storage was found to depend on their taxonomy (genus, species, strain) and the composition of the suspensi ...19761004279
identification of the cultivable bacteria in dental plaque from the beagle dog.fifteen different genera of microorganisms were isolated from the 27-month-old plaque of two beagle dogs. they were as follows: pseudomonas, proteus, neisseria, escherichia, staphylococcus, streptococcus, lactobacillus, nocardia, actinomyces, corynebacterium, micrococcus, microbacterium, brevibacterium, arthrobacter, and kurthia. the dog plaque was characterized by a low number of streptococci and the presence of several noncarbohydrate-fermenting organisms.19761069758
isolation of mutants with improved production of camp from microbacterium sp. no. 205 (atcc 21376).mutants were isolated from microbacterium sp. no. 205 (atcc 21376) producing 13-30 mm cyclic adenosine 3',5'-monophosphate (camp) by salvage biosynthesis, through sequential improvements of the bacterium for the purpose of improving camp production. the mutants produced 50-75 mm camp on 100 mm inosine 5'-monophosphate as a precursor. mutants resistant to the inhibition of growth by camp at high concentrations were isolated; the resistance was one of four characteristics effective for improved pr ...19901369318
culture media for non-sporulating gram-positive food spoilage bacteria.the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ...19921486021
aspei, a novel eam11051 isoschizomer from aureobacterium species recognizing 5'-gacnnn/nngtc-3'. 19921641345
chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi.linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ...19911657086
the specificity of bacterial siderophore receptors probed by bioassays.the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...19911663779
microbial metabolism of quinoline and related compounds. xi. degradation of quinoline-4-carboxylic acid by microbacterium sp. h2, agrobacterium sp. 1b and pimelobacter simplex 4b and 5b.from soil enrichment cultures four strains, using quinoline-4-carboxylic acid as sole source of energy and carbon, have been isolated. according to their physiological properties these bacteria have been identified as microbacterium sp. designated h2, as agrobacterium sp. designated 1b and pimelobacter simplex designated 4b and 5b. metabolites of the degradation pathway of quinoline-4-carboxylic acid have been isolated and identified. with pimelobacter simplex 4b and 5b 2-oxo-1,2-dihydroquinolin ...19911793516
transfructosylation of rebaudioside a (a sweet glycoside of stevia leaves) with microbacterium was found that a beta-fructofuranosidase produced by microbacterium sp. h-1 has potent trans-beta-fructofuranosylation activity from sucrose (donor). by means of this enzyme system, rebaudioside a (ra), the second major sweet steviol glycoside of the leaves of stevia rebaudiana, was subjected to transfructosylation, affording a mono-beta-fructofuranosylated product (ra-f) in a high yield. the structure of ra-f was elucidated as beta-d-fructofuranosyl-(2----6)-beta-d-glucopyranosyl ester of st ...19911797424
degradation of quinoline-4-carboxylic acid by microbacterium sp.from soil enrichment culture of quinoline-4-carboxylic acid-degrading bacterium was isolated. the organism was identified as microbacterium sp. mutants were induced with n-methyl-n'-nitro-n-nitrosoguanidine. one mutant accumulated successively two metabolites which were identified as 2-oxo-1,2-dihydro-quinoline-4-carboxylic acid and 8-hydroxy-2-oxo-2h-1-benzopyran-4-carboxylic acid.19892498156
phylogenetic analysis of the coryneform bacteria by 5s rrna sequences.nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ...19873106318
the thoracic surgical spectrum of acquired immune deficiency syndrome.the acquired immune deficiency syndrome has presented a complex and, as yet, unsolvable spectrum of pulmonary disease characterized by bizarre infections, pneumothoraces, respiratory distress, and death. thirty-eight patients underwent 49 surgical procedures during 42 months. ages of the patients ranged from 24 to 56 years. surgical procedures included tracheostomy, closure of air leaks, mediastinoscopy, lobectomy, open lung biopsy, and esophagogastrectomy. hospital mortality was 10 of 38 (24%); ...19863784593
microbacterium lacticum, troublemaker in blood fractions. 19744218564
cytochrome as a guide to classifying bacteria: taxonomy of microbacterium thermosphactum. 19684301060
production of coenzyme a by sarcina develop an efficient method for the production of coenzyme a (coa), optimal conditions for its formation from pantothenic acid, cysteine, and adenine were studied. a number of microorganisms were screened for production of coa. strains belonging to the genera sarcina, bacillus, microbacterium, micrococcus, and serratia accumulated coa. among these, sarcina lutea was selected as the best organism, and the culture conditions for the production of coa were investigated with this organism. under ...19744844273
numerical taxonomy of certain coryneform bacteria.da silva, g. a. nigel (iowa state university, ames), and john g. holt. numerical taxonomy of certain coryneform bacteria. j. bacteriol. 90:921-927. 1965-an electronic computer was used to sort 32 strains of coryneforms into groups with the tree-sort program. the similarity values obtained in this procedure were then used to construct a dendrogram depicting the phenetic resemblance among the taxa. the results indicated that all the phytopathogens studied were sufficiently distinct from the type s ...19654954898
[the murein types of the genus microbacterium]. 19705469569
a study of the lipid composition of microbacterium thermosphactum as a guide to its taxonomy. 19705491625
carbon dioxide fixation and the synthesis of aspartate by microbacterium thermosphactum. 19705495738
the lipid composition of microbacterium lacticum. 19685639272
effect of gamma irradiation on the microflora of freshwater fish. 3. spoilage patterns and extension of refrigerated storage life of yellow perch fillets irradiated to 0.1 and 0.2 megarad.maximal shelf life was determined and microbial flora were compared for irradiated (0.1 and 0.2 mrad) and nonirradiated yellow perch fillets stored at 1 c. shelf life was estimated by organoleptic determinations. microbiological studies included determination of the effects of irradiation on the total aerobic microbial population, lag phase, and rate of growth. genera of organisms isolated from fillets through the course of microbial spoilage were identified, and the proteolytic activity of the ...19685645411
[the amino acid sequence of the murein of microbacterium lacticum]. 19685650421
some morphological and physiological properties of microbacterium thermosphactum. 19685702042
a selective medium for the enumeration of microbacterium thermosphactum in meat and meat products. 19665980913
some observations on the taxonomy of the genus microbacterium. i. cultural and physiological reactions and heat resistance. 19665980923
some observations on the taxonomy of the genus microbacterium. ii. cell wall analysis, gel electrophoresis and serology. 19665980924
numerical taxonomy of gram-positive and catalase-positive rods isolated from frozen hundred isolates from peas, beans, and corn were compared with cultures of corynebacterium, microbacterium, and arthrobacter by use of numerical taxonomic procedures. six groups (clusters), representing 75% of the isolates, resembled corynebacteriaceae. there was some doubt regarding the genera represented because the groups were not closely related to many of the known strains. the relationship of the different groups to each other, as well as a number of their properties, is presented.19676031430
the amino acid sequence of a glycine-containing cell wall precursor of microbacterium lacticum. 19676033749
identification of threo-3-hydroxyglutamic acid in the cell wall of microbacterium lacticum. 19676072398
nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa.thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ...19826183393
a phylogenetic analysis of the family dermatophilaceae.the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ...19836195302
identification key for coryneform bacteria derived by numerical taxonomic studies.six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ...19836413644
energetics of growth of microbacterium thermosphactum at low temperatures.microbacterium thermosphactum was grown at 5 degrees c in glucose-limited continuous cultures. the end products of glucose metabolism were l-lactate and ethanol, and these compounds accounted for 86--92% of the glucose utilized. with input glucose concentrations less than 3 mv, yglumax was found to be 40--43, yatpmax 20--21 and ms 0.1--0.2. these values are almost identical to those found previously for cultures at 25 degrees c and show that this psychrotroph grows with a very high energetic eff ...19806782998
numerical classification of some rhodococci, corynebacteria and related organisms.nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ...19826811696
bacterial growth in ground beef prepared from electrically stimulated and nonstimulated muscles.ground beef samples prepared from electrically stimulated and nonstimulated biceps femoris and infraspinatus muscles were inoculated with lactobacillus sp., pseudomonas sp., acinetobacter sp., or a mixture of lactobacillus spp., pseudomonas spp., acinetobacter spp., moraxella sp., microbacterium thermosphactum, and erwinia herbicola. there were no significant differences in growth of various bacteria in ground beef made from electrically stimulated and nonstimulated muscles.19817195194
primary identification of microbacterium spp. encountered in clinical specimens as cdc coryneform group a-4 and a-5 bacteria.over nearly two decades, 13 yellow- or orange-pigmented, fermentative gram-positive rods belonging to the genus microbacterium were encountered in clinical specimens. all 13 strains, 10 of which came from blood cultures, were initially identified as cdc coryneform group a-4 and a-5 bacteria according to the scheme of hollis and weaver for the identification of gram-positive rods. the clinical isolates were compared with the type strains of the six species constituting the genus microbacterium as ...19957699039
formation of beta-fructosyl compounds of pyridoxine in growing culture of aspergillus niger.two pyridoxine compounds were found to be formed in a culture filtrate of aspergillus niger and a. sydowi, when grown in a medium containing sucrose and pyridoxine. each of the two compounds i and ii was obtained as a white powdered preparation by preparative paper chromatography, gel filtration on toyopearl hw-40s and sephadex g-10 columns, deae-cellulose column chromatography, and lyophilization. compounds i and ii were identified as 5'-o-(beta-d-fructofuranosyl)-pyridoxine and 5'-o-[beta-d-fr ...19937763873
primary identification of aureobacterium spp. isolated from clinical specimens as "corynebacterium aquaticum".over a 6-year period 11 yellow-pigmented gram-positive rods (gprs) with an oxidative carbohydrate metabolism were isolated from clinical specimens or were received as reference cultures and tentatively identified as "corynebacterium aquaticum" according to the guide of hollis and weaver for the differentiation of gprs (d. g. hollis and r. e. weaver, gram-positive organisms: a guide to identification, 1981). because these isolates seemed to be rather heterogeneous, comparative analyses with the t ...19947852557
an outbreak of ochrobactrum anthropi bacteraemia in five organ transplant patients.nosocomial bacteraemia caused by ochrobactrum anthropi occurred over a 1-month period in five organ transplant recipients, four of whom were in the same renal and pancreatic transplant unit. bacteraemia occurred with cyclosporin a, azathioprine and steroids, and with a rabbit anti-thymocyte globulin (ratg) during the induction phase. ratg appeared to be the only common factor among the five cases. over the period described, 71.4% of all patients receiving ratg developed o. anthropi bacteraemia. ...19947916361
in situ probing of gram-positive bacteria with high dna g + c content using 23s rrna-targeted oligonucleotides.23s-rrna-targeted oligonucleotide probes were designed for the phylogenetic group 'gram-positive bacteria with high g + c content of dna' (gpbhgc). a sequence idiosyncrasy in two adjacent base pairs in the stem of helix 69 in domain iv of the 23s rrna is present in all hitherto analysed strains of gpbhgc. an oligonucleotide probe targeted to this region hybridized only with strains of gpbhgc and was successfully used for in situ monitoring of these cells in activated sludge. another unique featu ...19948000548
suprageneric classification of peptidoglycan group b actinomycetes by nucleotide sequencing of 5s ribosomal rna.5s ribosomal rna sequences were determined for thirteen actinomycetes mainly representatives with the rare group b type peptidoglycan. the primary and secondary structure of the resultant sequences were of the type characteristic of gram-positive bacteria with dna rich in guanine plus cytosine. the sequencing and associated chemotaxonomic data provide compelling grounds for classifying actinomycetes with a group b type peptidoglycan in a single family. the family microbacteriaceae fam. nov. is p ...19938085792
polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge.eleven isolates obtained from a laboratory sewage treatment plant, most of them presumptively assigned to the coryneform genera curtobacterium and aureobacterium were studied for the presence of intracellular polyphosphates and polyphosphate dependent enzymes. all isolates stored polyphosphates and showed adenylate kinase activities ranging from 64 to 815 mu mg-1. polyphosphate:amp phosphotransferase could only be detected in one isolate. three isolates showed a polyphosphate kinase activity als ...19938386121
chemotaxonomy and molecular taxonomy of some coryneform clinical isolates.six reference strains of the genus brevibacterium as well as fifteen clinical isolates tentatively assigned to the genus using conventional biochemical methods, were the subject of chemotaxonomic and dna similarity studies. five of these clinical isolates were assigned either to the genera aureobacterium, mycobacterium, gordona or to rhodococcus on the basis of their dna mol% g+c, mycolic acid, amino-acid, sugar and menaquinone contents. among the ten remaining strains, six were not brevibacteri ...19938518510
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probesthe structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ...19968688004
purification and characterization of a prolidase from aureobacterium edta-insensitive prolidase (proline dipeptidase, ec was isolated from a cell-free extract of aureobacterium esteraromaticum ifo 3752. the enzyme was purified almost to homogeneity using acetone precipitation, hydrophobic chromatography, ion-exchange chromatography, and gel-permeation chromatography. the enzyme has a molecular weight of about 440,000 by gel permeation chromatography, and about 40,000 by sds polyacrylamide gel electrophoresis. the isoelectric point was 4.6. the enzyme ...19968782407
bioavailability of selenium accumulated by selenite-reducing bacteria.the bioavailability of selenium (se) was determined in bacterial strains that reduce selenite to red elemental se (seo). a laboratory strain of bacillus subtilis and a bacterial rod isolated from soil in the vicinity of the kesterson reservoir, san joaquin valley, ca, (microbacterium arborescens) were cultured in the presence of 1 mm sodium selenite (na2seo3). after harvest, the washed, lyophilized b. subtilis and m. arborescens samples contained 2.62 and 4.23% total se, respectively, which was ...19968811279
vancomycin-resistant aureobacterium species cellulitis and bacteremia in a patient with acute myelogenous leukemia.a 39-year-old male with acute myelogenous leukemia and concomitant porphyria cutanea tarda was admitted to the hospital for consolidation chemotherapy of his leukemia. during his hospitalization, he developed cellulitis of the left hand and persistent bacteremia with a yellow-pigmented, nonfermenting coryneform bacterium that was identified as aureobacterium sp. the portal of entry for the aureobacterium infection was probably through the skin lesions due to porphyria cutanea tarda. the infectio ...19968818896
an identification scheme for rapidly and aerobically growing gram-positive identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ...19968837385
the application of serological techniques to the taxonomy of arthrobacter and related organisms.antisera were raised against rods of 17 named arthrobacter and aureobacterium strains. antigenic relationships between these strains, other soil bacteria and new arthrobacter isolates from several soils were studied, using agglutination, immunodiffusion, immunofluorescence and elisa techniques. many of the named arthrobacter species had common antigens, and there were also common antigens amongst named arthrobacter strains and many fresh arthrobacter isolates. agglutination, elisa and immunofluo ...19968868431
degradation of prochloraz and 2,4,6-trichlorophenol by environmental bacterial strains.eight bacterial isolates from enrichment with 2,4,6-trichlorophenol (tcp) as sole carbon source were tested for their potential to degrade prochloraz. none of them could grow on prochloraz. strain c964, identified as aureobacterium sp., effectively reduced the fungitoxic activity of prochloraz in a bioassay and degradation was confirmed by hplc. two other isolates, strain c611 and c961, using tcp as a carbon source, belong to the beta subclass of the proteobacteria and presumely degrade tcp via ...19968920198
clinical microbiology of coryneform bacteria.coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ...19978993861
purification and characterization of dipeptidyl aminopeptidase from aureobacterium sp. wo26.we isolated a bacterial strain with an enzyme which releases dipeptide from gly-arg-p-nitroanilide. the bacterium was tentatively identified as aureobacterium sp. the enzyme, named audap, was purified and characterized. it was homogenous by sds-page and ief, and had a molecular mass of 90,000 da by sds-page and 88,000 da by gel filtration, so it may be a monomer. the isoelectric point was 3.8 and the optimum ph was 10.0. the purified enzyme hydrolyzed gly-arg-pna, a model substrate for dap i, an ...19979028041
endophthalmitis due to microbacterium species: case report and review of microbacterium infections.microbacterium species (formerly cdc [centers for disease control and prevention] coryneform group a-4 and a-5 bacteria) are widely distributed in the environment and rarely cause infections in humans. we present a case of endophthalmitis due to microbacterium species that occurred after accidental trauma and review the literature on microbacterium infections. if the infected tissue or medical device is removed and antimicrobial therapy (preferably with beta-lactams or glycopeptides) is institut ...19979145748
[microflora of the air in sawmills as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens].microbiologial studies of the air and allergological examinations of the workers were performed in two sawmills processing deciduous wood (mainly oak) and in one sawmill processing coniferous wood (mainly pine). the concentration of microorganisms in the air was of the order 10(3)-10(4) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium), spore-forming bacilli (bacillus), gram-negative bacteria (rahnella) and filamentous fungi (as ...19969190234
[microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens].microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ...19969190236
multicenter evaluation of the updated and extended api (rapid) coryne database a multicenter study, 407 strains of coryneform bacteria were tested with the updated and extended api (rapid) coryne system with database 2.0 (biomérieux, la-balme-les-grottes, france) in order to evaluate the system's capability of identifying these bacteria. the design of the system was exactly the same as for the previous api (rapid) coryne strip with database 1.0, i.e., the 20 biochemical reactions covered were identical, but database 2.0 included both more taxa and additional differentia ...19979399506
thermostable phenylalanine dehydrogenase from a mesophilic microbacterium sp. strain dm 86-1bacteria that produced nad+-dependent phenylalanine dehydrogenase (ec were selected among l-methionine utilizers isolated from soil. a bacterial strain showing phenylalanine dehydrogenase activity was chosen and classified in the genus microbacterium. phenylalanine dehydrogenase was purified from the crude extract of microbacterium sp. strain dm 86-1 (tpu 3592) to homogeneity as judged by sds-polyacrylamide disc gel electrophoresis. the enzyme has an isoelectric point of 5.8 and a rela ...19989477256
psychrotrophic bacteria isolated from a constantly warm tropical environment.psychrotrophic bacteria are known to occur in temperate, constantly cold, and artificially cooled environments. this is the first report of their occurrence in a constantly warm (ca. 24 degrees-35 degrees c) tropical environment. soil samples taken from two sites along the southeastern coastal zone of jamaica yielded growth of psychotrophic bacteria after 3-4 weeks of enrichment culture in 1/30 strength tryptic soy broth, 20 mg l-1 cycloheximide at 2 degrees c. growth of individual isolates at 2 ...19989516542
biodegradation of metal-edta complexes by an enriched microbial population.a mixed culture utilizing edta as the sole carbon source was isolated from a mixed inoculum of water from the river mersey (united kingdom) and sludge from an industrial effluent treatment plant. fourteen component organisms were isolated from the culture, including representatives of the genera methylobacterium, variovorax, enterobacter, aureobacterium, and bacillus. the mixed culture biodegraded metal-edta complexes slowly; the biodegradability was in the order fe > cu > co > ni > cd. by incor ...19989546167
serum antibodies reacting with subgingival species in refractory periodontitis subjects.the purpose of this investigation was to compare the levels of serum igg antibody to 85 subgingival species in 32 refractory periodontitis, 56 successfully treated, and 33 periodontally healthy subjects. refractory subjects showed mean full mouth attachment loss and/or >3 sites showing attachment loss >2.5 mm within 1 year after 2 treatment modalities, scaling and root planing and surgery plus systemically administered tetracycline. successfully-treated subjects showed mean attachment level gain ...19989696261
microbial communities of printing paper machines.the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ...19989717292
union of the genera microbacterium orla-jensen and aureobacterium collins et al. in a redefined genus microbacterium.the 16s rrna gene sequences of 19 strains, 11 strains representing validated aureobacterium or microbacterium species and eight strains of non-valid species or isolates, were determined. these sequences were aligned with the sequences of other validated aureobacterium and microbacterium species and related actinobacteria. a comparative sequence analysis of 43 strains revealed that the species of the genera aureobacterium and microbacterium form a monophyletic association in which species of both ...19989734028
etherolytic cleavage of 4-(2,4-dichlorophenoxy)butyric acid and 4-(4-chloro-2-methylphenoxy)butyric acid by species of rhodococcus and aureobacterium isolated from an alkaline environment.bacterial strains were isolated from the concrete rubble of a demolished herbicide production plant. the predominant feature of these strains was the etherolytic cleavage of 4-(2,4-dichlorophenoxy)butyric acid (dcpb)1) and 4-(4-chloro-2-methylphenoxy)butyric acid (mcpb) while liberating 2,4-dichlorophenol (dcp) and 4-chloro-2-methylphenol (mcp) respectively. some of the isolates were identified by 16s rdna sequence analysis and shown to belong to the genera aureobacterium sp. (strain k2-17) and ...19989867509
cloning of a novel prolidase gene from aureobacterium esteraromaticum.the prolidase gene from aureobacterium esteraromaticum was cloned and expressed in escherichia coli. the cloned enzyme had the same enzymatic properties as the wild-type enzyme. kinetic analysis of the enzyme indicated that the best substrate was pro-hyp, which was not hydrolyzed by other prolidases. interestingly, there was no homology between the deduced amino acid sequence of a. esteraromaticum prolidase and those of the other sources such as human e. coli and lactobacillus. however, homology ...19999989239
effect of elevated oxygen and carbon dioxide on the surface growth of vegetable-associated micro-organisms.the impact of a novel type of modified atmosphere (ma), referred to as high o2-ma, on micro-organisms associated with the spoilage of minimally-processed vegetables was studied. pure cultures of pseudomonas fluorescens, enterobacter agglomerans, aureobacterium strain 27, candida guilliermondii, c. sake, salmonella typhimurium, salm. enteritidis, escherichia coli, listeria monocytogenes, leuconostoc mesenteroides var. mesenteroides, lactobacillus plantarum and lactococcus lactis were cultured on ...199910196748
actinomycetes in karstic caves of northern spain (altamira and tito bustillo).a variety of isolation procedures were carried out to study the involvement of bacteria in the colonisation and biodeterioration of spanish caves with paleolithic rock art (altamira and tito bustillo). the applied techniques mainly aimed to isolate heterotrophic bacteria such as streptomycetes, nocardioform and coryneform actinomycetes, and other gram-positive and gram-negative bacteria. the results demonstrated that actinomycetes were the most abundant gram-positive bacteria in the caves. actin ...199910353805
analysis of the bacterial surface ripening flora of german and french smeared cheeses with respect to their anti-listerial potential.the anti-listerial potential of 19 different french smeared cheese bacterial consortia was analyzed semi-quantitatively. comparison of the total viable cell count to the direct microscopic count yielded no indication that non-culturable bacteria contribute significantly to the undefined, complex ripening floras. from 2613 isolates, 48 showed clear inhibition of one or more listeria monocytogenes strains on solid medium, while only three isolates excreted an anti-listerial, soluble substance when ...199910357277
aureobacterium masquerading as 'corynebacterium aquaticum' infection: case report and review of the literature.a gram-positive bacillus was isolated repeatedly from blood taken through the lumina of a central venous catheter of a patient with multiple myeloma who developed febrile neutropenia following chemotherapy. the bacterium was identified by the api coryne system as 'corynebacterium aquaticum'. gene analysis targeting the 16s rrna indicated that the organism had a 99.5% identity with aureobacterium liquefaciens although there were two phenotypic characteristics at variance with the description of t ...199910510974
microbacterium kitamiense sp. nov., a new polysaccharide-producing bacterium isolated from the wastewater of a sugar-beet factory.two strictly aerobic, heterotrophic and mesophilic new organisms, strains kitami a1 and kitami c2t, were isolated from the wastewater of a sugar-beet factory in kitami city, hokkaido, japan. in batch cultures, these organisms produced both insoluble and soluble exopolysaccharides (epss) utilizing sucrose as the sole carbon source. the g + c contents of the strains kitami c2t and kitami a1 were 69.2 mol%. both strains had anteiso-c15:0 acid, anteiso-c17:0 acid and iso-c16:0 as major components. t ...199910555312
high resistance to oxygen radicals and heat is caused by a galactoglycerolipid in microbacterium sp. m874.microbacterium sp. m874 produced a glyceroglycolipid, di-o-12-methyl-tetradecanoyl-3-o-beta-d-galactopyranosyl-sn-glycerol, at about the 50 microm level. though the strain was highly resistant to tertiary-butyl hydroperoxide (tbhp) in a glycolipid-productive medium, the resistance was reduced in a nonproductive medium. exogenous addition of the glycolipid to the nonproductive culture restored the resistance. this addition also increased the resistance to heat, ethanol, and 4-chloro-1-naphthol, i ...200010788780
production and structure elucidation of glycoglycerolipids from a marine sponge-associated microbacterium species.the bacterium microbacterium sp., isolated from the sponge halichondria panicea, produced four unusual cell-associated glycoglycerolipids and one diphosphatidylglycerol when grown on marine broth and on artificial seawater media. the lipids were isolated by chromatography on silica columns and their structures elucidated using a combination of multidimensional nmr and ms techniques. the main compound was 1-o-acyl-3-[alpha-glucopyranosyl-(1-3)-(6-o-acyl-alpha-mannopyranosyl )]glycerol (ggl.2) wit ...200010843572
comparison of colony lift with direct spotting methods of blot preparation on the effect of colony hybridization in the detection of environmental organisms.nucleic acid probes are used on site to detect or to identify individual microbial cells without cultivation. this molecular technique can avoid some limitations of traditional identification methods including time consuming and imprecise. this study examined the factors affecting colony hybridization and compared the effectiveness of membrane prepared by colony lifting with direct spotting procedures using the universal probe eub 338. the results of hybridization varied depending on the type of ...200010917884
overexpression, purification, and characterization of bacillus subtilis n-acetylmuramoyl-l-alanine amidase cwlc.n-acetylmuramoyl-l-alanine amidase cwlc of bacillus subtilis was overproduced in escherichia coli and purified 21-fold. the amidase hydrolyzed type a cell walls such as b. subtilis. the amidase bound slightly to the microbacterium lacticum cell wall (type b), but did not entirely hydrolyze it. the presence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation.200010945275
microbial diversity during maturation and natural processing of coffee cherries of coffea arabica in brazil.the magnitude and diversity of the microbial population associated with dry (natural) processing of coffee (coffea arabica) has been assessed during a 2-year period on 15 different farms in the sul de minas region of brazil. peptone water-washed samples were taken of maturing cherries on trees (cherries, raisins and dried cherries) and from ground fermentations. the microbial load varied from 3 x 10(4) to 2.2 x 10(9) cfu/cherry with a median value of 1.6 x 10(7) cfu/cherry. the microbial load in ...200011016614
identification of corynebacterium bovis and other coryneforms isolated from bovine mammary glands.bovine mastitis remains the most economically important disease in dairy cows. corynebacterium bovis, a lipid-requiring corynebacterium spp., is frequently isolated from the milk of infected mammary glands of dairy cows and is associated with reduced milk production. a total of 212 coryneform bacteria isolated from the milk of dairy cows were obtained from mastitis reference laboratories in the united states and canada. all isolates had been presumptively identified as corynebacterium bovis base ...200011049082
a novel bacterial pathogen, microbacterium nematophilum, induces morphological change in the nematode c. elegans.the dar (deformed anal region) phenotype, characterized by a distinctive swollen tail, was first detected in a variant strain of caenorhabditis elegans which appeared spontaneously in 1986 during routine genetic crosses [1,2]. dar isolates were initially analysed as morphological mutants, but we report here that two independent isolates carry an unusual bacterial infection different from those previously described [3], which is the cause of the dar phenotype. the infectious agent is a new specie ...200011137017
enumeration and characterization of cellulolytic bacteria from refuse of a landfill.enumeration and phenotypic characterization of aerobic cellulolytic bacteria were performed on fresh, 1 year old and 5 years old refuse samples of a french landfill site. numbers of cellulolytic bacteria ranged from 1.1x10(6) to 2.3x10(8) c.f.u. (g dry wt.)(-1) and were lower in 5 years old refuse samples. a numerical analysis of phenotypic data based on 80 biochemical tests and performed on 321 gram-positive isolates from refuse, revealed a high phenotypic diversity of cellulolytic bacteria whi ...200111137603
resident aerobic microbiota of the adult human nasal cavity.recent evidence strongly suggests that the microbiota of the nasal cavity plays a crucial role in determining the reaction patterns of the mucosal and systemic immune system. however, little is known about the normal microbiota of the nasal cavity. the purpose of this study was to determine the microbiota in different parts of the nasal cavity and to develop and evaluate methods for this purpose. samples were collected from 10 healthy adults by nasal washes and by swabbing of the mucosa through ...200011200821
rescuing activity of galactoglycerolipids from cellular lesions induced by 5-aminolevulinic anti-oxygen radical reagent of a bacterial metabolite, m874 monogalactoglycerolipid (di-o-12-methyl-tetradecanoyl-3-o-beta-d-galactopyranosyl-sn-glycerol ), was tested for its ability to protect two organisms against cellular lesions induced by 5-aminolevulinic acid (ala) and light. in corynebacterium flavescens atcc 10340, extracellular uroporphyrin and coproporphyrin were the main porphyrin products. although less than 2 mm ala increased porphyrin synthesis, ala levels above 3 mm inhibited ...200010788790
identification of coryneform bacterial isolates by ribosomal dna sequence analysis.identification of coryneform bacteria to the species level is important in certain circumstances for differentiating contamination and/or colonization from infection, which influences decisions regarding clinical intervention. however, methods currently used in clinical microbiology laboratories for the species identification of coryneform bacteria are often inadequate. we evaluated the microseq 500 16s bacterial sequencing kit (perkin-elmer biosystems, foster city, calif.), which is designed to ...200010747168
nosocomial outbreak of microbacterium species bacteremia among cancer date, only 6 sporadic microbacterium species (formerly coryneform centers for disease control and prevention [cdc] groups a-4 and a-5) infections have been reported. the source, mode of transmission, morbidity, mortality, and potential for nosocomial transmission of microbacterium species remain unknown. from 26 july through 14 august 1997, 8 episodes of coryneform cdc group a-5 symptomatic bacteremia occurred in 6 patients on the oncology ward at the maine medical center. one patient died. a ...200111517437
purification and properties of pyridoxine oxidase from aureobacterium luteolum and pyridoxal reductase from schizosaccharomyces pombe. 200010705982
chromate reduction and 16s rrna identification of bacteria isolated from a cr(vi)-contaminated site.a gram-positive, hexavalent chromium [chromate: cr(vi)]-tolerant bacterium, isolated from tannery waste from pakistan, was identified as a microbacterium sp. by 16s rrna gene sequence homology. the strain (designated as mp30) reduced toxic cr(vi) only under anaerobic conditions at the expense of acetate as the electron donor. the bacterium was able to grow aerobically in l-broth supplemented with 15 mm cro4(2-) but then did not reduce cr(vi). at a concentration of 2.4x10(9) cells/ml, 100 microm ...200111693930
microbacterium gubbeenense sp. nov., from the surface of a smear-ripened cheese.phenotypic and phylogenetic studies were performed on 11 strains of a microbacterium-like organism isolated from the surface of a smear-ripened cheese. the isolates were gram-positive, catalase-positive, facultatively anaerobic, oxidase-negative, non-spore-forming, non-motile, small, slender rods and grew in 12% (w/v) nacl. chemotaxonomic investigation revealed that all the isolates belonged unambiguously to the genus microbacterium. they contained type b1 peptidoglycans with l-lysine as the dia ...200111760936
analysis of bacterial dna in skin and muscle of the tyrolean iceman offers new insight into the mummification process.about 80 sequences (16s ribosomal rna gene) of bacterial dna in samples of skin and muscle taken directly from the tyrolean iceman (3350-3100 years b.c.) or recovered during the 1992 archaeological expedition at the alpine site were analyzed to obtain clues to the natural mummification process that allowed the corpse of the neolithic shepherd/hunter to be preserved for more than 5,000 years. the investigation was made more complex by the fact that the surface of the mummy had been swabbed with p ...200010640948
facile enzymatic conversion of lactose into lacto-n-tetraose and lacto-n-neotetraose.lacto-n-tetraose (galbeta1 -3glcnacbeta1-3galbeta1-4glc, lnt) and lacto-n-neotetraose (galbeta1-4glcnacbeta1-3galbeta1-4glc, lnnt) were enzymatically synthesized by consecutive additions of glcnac and gal residues to lactose. lacto-n-triose ii (glcnacbeta1-3galbeta1-4glc) was prepared first by the transfer of glcnac from udp-glcnac to lactose by beta-1,3-n-acetylglucosaminyltransferase from bovine serum. the resulting lacto-n-triose ii was converted into lnt and lnnt utilizing two kinds of beta- ...199910596893
effects of combined shear and thermal forces on destruction of microbacterium lacticum.a twin-screw extruder and a rotational rheometer were used to generate shear forces in concentrated gelatin inoculated with a heat-resistant isolate of a vegetative bacterial species, microbacterium lacticum. shear forces in the extruder were mainly controlled by varying the water feed rate. the water content of the extrudates changed between 19 and 45% (wet weight basis). higher shear forces generated at low water contents and the calculated die wall shear stress correlated strongly with bacter ...199910508076
aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite zootermopsis demonstrate the occurrence of cellulolytic bacteria in the termite zootermopsis angusticollis.200211849325
arsenic (iii) oxidizing microbacterium lacticum and its use in the treatment of arsenic contaminated develop a microbially-assisted process for the removal of arsenic from contaminated groundwater.200211940155
reclassification of brevibacterium oxydans (chatelain and second 1966) as microbacterium oxydans comb. nov.phylogenetic and chemotaxonomic analyses indicate that brevibacterium oxydans is closely related to species of the genus microbacterium, namely microbacterium liquefaciens, microbacterium luteolum and microbacterium saperdae. dna-dna reassociation values of less than 60% between brevibacterium oxydans and these three microbacterium species support the distinctness of this misclassified brevibacterium species, which is reclassified as microbacterium oxydans comb. nov.199910028259
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