survival and reversion of a stable l form in soil.the stable l form of agromyces ramosus reverted to a bacterial form when incubated in sterilized soil. the cellular and colonial morphology of this bacterial form resembled that of the original parent bacterial form. the two forms differed, however, in that the revertant maintained its bacterial form when transferred onto a low-salt (nacl) medium but was virtually completely induced into the l-form state on a high-salt medium. the original parent bacterial form was not sensitive to salt. the pos ...1978754877
small cells in pure cultures of agromyces ramosus and in natural soil.previously, transmission electron-microscopic observations of thin-sectioned and frozen-etched preparations of microorganisms concentrated directly from soil showed many cells that were considerably smaller than the cells found in pure cultures isolated from soil. the present study shows that pure cultures of the soil bacterium agromyces ramosus can produce cells as small as those observed in natural soil. growth on a nutritionally limiting medium and the presence of an agar surface contributed ...1977837257
l-phase variants of agromyces ramosus.agromyces ramosus, which is a numerically prevalent bacterium in soil, was easily induced into the l-phase by growing it on agar media containing low levels of penicillin or glycine. the l-forms were stable after initial contact with the inducing agent and could not be reverted to the bacterial form by any of the procedures tried. these results are discussed in relation to a possible natural occurrence of l-forms of this bacterium in soil.19751080392
[amino acid sequence of 2,4-diaminobutyric acid-containing mureins of various coryneform bacteria and agromyces ramosus]. 19734693054
cytochrome composition and effect of catalase on growth of agromyces ramnosus. 19705422600
nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa.thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ...19826183393
suprageneric classification of peptidoglycan group b actinomycetes by nucleotide sequencing of 5s ribosomal rna.5s ribosomal rna sequences were determined for thirteen actinomycetes mainly representatives with the rare group b type peptidoglycan. the primary and secondary structure of the resultant sequences were of the type characteristic of gram-positive bacteria with dna rich in guanine plus cytosine. the sequencing and associated chemotaxonomic data provide compelling grounds for classifying actinomycetes with a group b type peptidoglycan in a single family. the family microbacteriaceae fam. nov. is p ...19938085792
agromyces mediolanus sp. nov., nom. rev., comb. nov., a species for "corynebacterium mediolanum" mamoli 1939 and for some aniline-assimilating bacteria which contain 2,4-diaminobutyric acid in the cell wall the course of identifying aniline-assimilating bacteria, researchers found some gram-positive strains that contain 2,4-diaminobutyric acid in their cell wall peptidoglycans and menaquinone 12 as the predominant menaquinone. "corynebacterium mediolanum" and "flavobacterium dehydrogenans" also are known to contain 2,4-diaminobutyric acid in their cell walls, as well as menaquinone 12, but the taxonomic position of these organisms has not been established previously. we found that the aniline-as ...19968573525
distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes.mycothiol [2-(n-acetylcysteinyl)amido-2-deoxy-alpha-d-glucopyranosyl- (1-->1)-myo-inositol] (msh) has recently been identified as a major thiol in a number of actinomycetes (s. sakuda, z.-y. zhou, and y. yamada, biosci. biotech. biochem. 58:1347-1348, 1994; h. s. c. spies and d. j. steenkamp, eur. j. biochem. 224:203-213, 1994; and g. l. newton, c. a. bewley, t. j. dwyer, r. horn, y. aharonowitz, g. cohen, j. davies, d. j. faulkner, and r. c. fahey, eur. j. biochem. 230:821-825, 1995). since thi ...19968606174
microbiology of the stalactites from grotta dei cervi, porto badisco, italy.the active stalactites from grotta dei cervi, porto badisco, southeastern italy, were sampled to investigate the microbial communities present in these speleothems. sampling was carried out in a transect about 150 m long in the central gallery, where numerous gram-positive bacteria were isolated. actinomycetes of the genus streptomyces were the most abundant, followed by members of the genus bacillus. further isolates were assigned to the genera amycolatopsis, arthrobacter; agromyces. micrococcu ...200010963330
precipitin response of potato processing workers to work-related microbial allergens.serum samples from 61 potato processing workers and 30 urban dwellers not exposed to organic dusts (as a reference group) were examined in agar-gel precipitation test performed by ouchterlony double diffusion method with the antigens of 12 microorganisms associated with organic dusts. each serum was tested twice: not concentrated, and three-fold concentrated, for the detection of low levels of precipitins. the antibody response of workers to the antigen of coryneform bacterium agromyces ramosus ...200212498593
leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate "corynebacterium aquaticum" leifson 1962 and clavibacter xyli subsp. cynodontis davis et al. 1984."corynebacterium aquaticum" was first proposed by leifson in 1962 but not included in the approved lists of bacterial names in 1980. this species has been left from reclassification of the genus corynebacterium because of the unusual chemotaxonomic characteristics such as 2,4-diaminobutyric acid (dab) in the peptidoglycan and menaquinones of mk-10 and mk-11. a close relationship of "c. aquaticum" to the genera agromyces and rathayibacter has been pointed out from the viewpoint of chemotaxonomic ...199912501368
reclassification of agromyces fucosus subsp. hippuratus as agromyces hippuratus sp. nov., comb. nov. and emended description of agromyces fucosus.the taxonomic position of agromyces fucosus subsp. hippuratus is revised on the basis of molecular and phenotypic data. phylogenetic analysis based on 16s rrna gene sequences, dna-dna relatedness values and differences from other species in phenotypic traits revealed in this and earlier studies suggested reclassification of a. fucosus subsp. hippuratus as a separate species, agromyces hippuratus sp. nov., comb. nov. the type strain is vkm ac-1352t (= jcm 9087t). an emended description of agromyc ...200415388709
[the ability of saprotrophic bacteria isolated from natural habitats to lyse yeasts].more than 600 bacterial strains isolated from different horizons of steppe biogeocenoses and zoogenous loci (diplopod intestines and feces) were tested for the ability to lyse yeast cell walls. about half of the strains that were isolated from biotopes with active degradation of plant debris (steppe litters and diplopod intestines and feces) were found to possess yeast-lytic activity. most of the yeast-lytic strains belonged to the genera streptomyces, promicromonospora, oerskovia, and agromyces ...200415521184
agromyces italicus sp. nov., agromyces humatus sp. nov. and agromyces lapidis sp. nov., isolated from roman catacombs.a polyphasic study was carried out to clarify the taxonomic positions of three gram-positive isolates from the catacombs of domitilla, rome (italy). 16s rrna gene sequence comparisons placed these strains within the genus agromyces. the morphological and chemotaxonomic characteristics of these isolates were consistent with the description of the genus agromyces. the three isolates could be readily distinguished from one another and from representatives of all agromyces species with validly publi ...200515774677
[isoprenoid pigments in representatives of the family microbacteriaceae].by using fosmidomycin and mevinolin (inhibitors of the synthesis of isoprenoid pigments), spectrophotometry, and mass spectrometry, the presence of isoprenoid pigments is shown in 71 of the 78 strains under study. all of these strains belong to 11 genera of the family microbacteriaceae. yellow, orange, and red pigments are found to have absorption spectra typical of c40-carotenoids. eight out of the sixteen strains of the genus microbacterium are able to synthesize neurosporene, a precursor of l ...200516119846
interaction of agromyces ramosus with other bacteria in soil.agromyces ramosus occurs in very high numbers in most soils and, based on studies of laboratory isolates, does not require host cells for growth. nevertheless, it attacked and destroyed most of the gram-positive and gram-negative bacterial species tested as possible host organisms. a. ramosus also attacked and destroyed saccharomyces cerevisiae. the possibility of attack on fungi was unclear. among the bacteria serving as hosts were the important soil species azotobacter vinelandii, rhizobium le ...198316346402
relation to copper of n-1, a nonobligate bacterial predator.nonobligate bacterial predator strain n-1 was highly resistant to copper. in fact, it required more than minimal amounts of copper to initiate growth, but not for growth that followed growth initiation. strain n-1 made a peptide growth initiation factor (gif) to marshal copper from its environment for growth initiation. production of this gif occurred before the onset of growth initiation, but production was shut down if excess copper was present. at high copper levels, the time required for ons ...198716347382
diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh-like gene within the genera microbacterium and starkeya.we characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of tunisia by 16s rdna polymerase chain reaction (pcr)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), 16s rdna and 16s-23s rdna internal transcribed spacer sequencing. phylogenetically, these isolates belong to the branches containing the genera inquilinus, bosea, rhodopseudomonas, para ...200616598639
a role for carbon catabolite repression in the metabolism of phosphonoacetate by agromyces fucosus vs2.a strain of agromyces fucosus, designated vs2, metabolized a range of organophosphonate compounds as sole phosphorus sources for growth and metabolized phosphonoacetate as a sole carbon, energy and phosphorus source for growth. with phosphonoacetate as the sole phosphorus source and a pyruvate carbon source, transient phosphate release to the medium was observed, in contrast to cultures grown with glucose and phosphonoacetate, where no phosphate release to the medium was observed. carbon catabol ...200616842370
[bacterial communities of brown and red algae from peter the great bay, the sea of japan].the structure of microbial communities of brown algae, red algae, and of the red alga gracilaria verrucosa, healthy and affected with rotten thallus, were comparatively investigated; 61 strains of heterotrophic bacteria were isolated and characterized. most of them were identified to the genus level, some vibrio spp., to the species level according to their phenotypic properties and the fatty acid composition of cellular lipids. the composition of the microflora of two species of brown algae was ...200616871810
agromyces allii sp. nov., isolated from the rhizosphere of allium victorialis var. platyphyllum.two gram-positive, non-motile, yellow-pigmented, slightly curved and rod-shaped bacterial strains, ums-62(t) and ums-101, were isolated from the rhizosphere of allium victorialis var. platyphyllum, a variety of wild edible greens grown on ulleung island, korea. the taxonomic position of the strains was investigated by a polyphasic approach. strains ums-62(t) and ums-101 grew optimally at 30 degrees c and at ph 6.5-7.5. the novel strains contained mk-11 and mk-12 as the predominant menaquinones a ...200717329790
rt-tgge as a guide for the successful isolation of phosphonoacetate degrading bacteria.use of molecular techniques for the isolation of bacteria capable of phosphonoacetate mineralization as carbon, phosphorus and energy source.200717584470
6-aminohexanoate oligomer hydrolases from the alkalophilic bacteria agromyces sp. strain ky5r and kocuria sp. strain ky2.alkalophilic, nylon oligomer-degrading strains, agromyces sp. and kocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge from a sewage disposal plant. the 6-aminohexanoate oligomer hydrolases (nylc) from the alkalophilic strains had 95.8 to 98.6% similarity to the enzyme in neutrophilic arthrobacter sp. but had superior thermostability, activity under alkaline conditions, and affinity for nylon-related substrates, which would be advantageous for biotechnolo ...200717827307
[phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china].the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ...200717944352
[microbial diversity in scorpion intestine (buthus martensii karsch)].scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ...200718062268
genetic organization of nylon-oligomer-degrading enzymes from alkalophilic bacterium, agromyces sp. ky5r.a 15-kb gene locus including nylon-oligomer-degrading genes from the chromosome of an alkalophilic bacterium, agromyces sp. ky5r, was cloned and sequenced. the genetic organization was similar to the dna region flanked by directly repeated is6100 sequences on the nylon-oligomer-degradative plasmid poad2. however, we found several genetic rearrangements between the two dna regions. here, we discuss the possible mechanisms underlying the genetic rearrangements.200718215642
interactions of plant-beneficial bacteria with the ascomycete coniochaeta assess the interactions between coniochaeta ligniaria f/tgf15 obtained from torrefied grass fibers (tgf) and selected bacteria from the same substrate.200919298515
agromyces tropicus sp. nov., isolated from soil.two actinomycete strains, cm9-9(t) and ak2-48, which produced straight rod-shaped, non-motile cells, were isolated from soils in chiang mai and phuket provinces, respectively, thailand. the morphological and chemotaxonomic characteristics of the isolates coincided with those of the genus agromyces. phylogenetic analysis using 16s rrna gene sequences also indicated that the isolates were clearly separated from their closest relative, agromyces aurantiacus yim 21741(t), and should be classified in ...201120435752
agromyces flavus sp. nov., an actinomycete isolated from a soil sample from qinghai-tibet plateau, china.a gram-positive, non-motile strain, designated cpcc 202695t, was isolated from a soil sample collected from qinghai-tibet plateau, northwest of china. the strain cpcc 202695t contained rhamnose, glucose and galactose in the cell wall as diagnostic sugars, and 2,4-diaminobutyric acid, alanine, glutamic acid and glycine in the peptidoglycan. the polar lipids consisted of diphosphatidylglycerol (dpg), phosphatidylglycerol (pg) and two unknown glycolipids (gl). mk-12 was the predominant menaquinone, ...201020802064
pyrosequencing-based assessment of bacterial community structure along different management types in german forest and grassland soils.soil bacteria are important drivers for nearly all biogeochemical cycles in terrestrial ecosystems and participate in most nutrient transformations in soil. in contrast to the importance of soil bacteria for ecosystem functioning, we understand little how different management types affect the soil bacterial community composition.201121359220
agromyces atrinae sp. nov., isolated from fermented seafood.a gram-staining-positive, aerobic, non-motile and rod-shaped bacterium, designated p27(t), was isolated from a traditional fermented seafood. the isolate grew optimally with 0-2.0 % (w/v) nacl and at ph 6-7 and 30 degrees c. the predominant menaquinones were mk-12 and mk-11. the major cellular fatty acids were anteiso-c(17 : 0), anteiso-c(15 : 0) and iso-c(16 : 0). the major cell-wall sugars were galactose, mannose and rhamnose. the peptidoglycan amino acids of strain p27(t) were 2,4-diaminobuty ...201019666805
interactions between accumulation of trace elements and macronutrients in salix caprea after inoculation with rhizosphere microorganisms.although the beneficial effects on growth and trace element accumulation in salix spp. inoculated with microbes are well known, little information is available on the interactions among trace elements and macronutrients. the main purpose of this study was to assess the effect of phytoaugmentation with the rhizobacteria agromyces sp., streptomyces sp., and the combination of each of them with the fungus cadophora finlandica on biomass production and the accumulation of selected trace elements (zn ...201121612812
genes from a dietzia sp. for synthesis of c40 and c50 beta-cyclic carotenoids.dietzia sp. cq4 accumulated the c(40) beta-cyclic carotenoids (canthaxanthin and echinenone) and the c(50) beta-cyclic carotenoid (c.p.450 monoglucoside). a plant-type lycopene beta-cyclase gene crtl was identified for beta-cyclization of the c(40) carotenoids. a carotenoid synthesis gene cluster was identified away from the crtl gene, which contained the crtebi genes for the synthesis of lycopene followed by the lbtabc genes for lycopene elongation and beta-cyclization of the c(50) carotenoids. ...200717008032
crystallization and x-ray diffraction analysis of nylon-oligomer hydrolase (nylc) from agromyces sp. ky5r.6-aminohexanoate-oligomer hydrolase (nylc) from agromyces sp. ky5r was-áexpressed in escherichia coli jm109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. nylc was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as a precipitant in 0.1ôçàm hepes buffer ph 7.5 containing 0.2ôçàm nacl. diffraction data were collected from native and k(2)ptcl(4)-derivative crystals to resolutions of 2.00 and 2.20ôç ...201121821888
agromyces subbeticus sp. nov., isolated from a cave in southern actinomycete, strain z33(t), was isolated from a cyanobacterial biofilm in the cave of bats, near zuheros (cordoba, southern spain). 16s rrna gene sequence analysis showed that strain z33(t) formed a distinct phyletic line within the genus agromyces. this isolate could be readily distinguished from representatives of all recognized agromyces species on the basis of a broad range of phenotypic characteristics and dna-dna relatedness data. genotypic and phenotypic properties indicate that strai ...200516166685
agromyces ulmi sp. nov., a xylanolytic bacterium isolated from ulmus nigra in spain.two xylan-degrading bacterial strains were isolated from a decayed ulmus nigra tree in spain. the isolates were gram-positive, non-motile, aerobic and formed substrate mycelium which fragmented into irregular rods. 16s rrna gene sequence analysis indicated that the isolates form a separate branch within the genus agromyces phylogenetic cluster, with agromyces mediolanus dsm 20152(t) being their closest relative (97.7 and 97.6 % sequence similarity). catalase, nitrate reduction and urease tests d ...200415545422
agromyces aurantiacus sp. nov., isolated from a chinese primeval forest.a catalase-negative actinomycete, strain yim 21741t, was isolated from a soil sample collected from a primeval forest at xishuangbanna, yunnan province, china. analysis of 16s rdna showed the strain to be related to members of the genus agromyces, with which it also shares morphological characteristics, e.g. branching hyphae breaking into diphtheroid and rod-like, irregular, non-motile fragments and a peptidoglycan containing the diagnostic amino acid 2,4-diamino-n-butyric acid. whole-cell hydro ...200312656188
poly(arabitol phosphate) teichoic acid in the cell wall of agromyces cerinus subsp. cerinus vkm ac-1340t.on the basis of nmr studies and analysis of the products of acid and alkaline hydrolyses the following structures were established for the repeating units of poly(arabitol phosphate) teichoic acid: alpha-6-deoxy-l-talp-(1-->3)-beta-d-glcpnac-(1-->2)-alpha-l-rha p-(1-->4(2)-d- arabitol-po4 and beta-d-glcpnac-(1-->2)-alpha-l-rhap-(1-->4(2)-d-arabitol-po4. the molar ratio of these units is about 1.2:1.0, respectively. poly(arabitol phosphate) teichoic acid is here reported in bacterial cells walls ...19957672119
microbial metabolism of carbon monoxide in culture and in soil.nocardia salmonicolor readily oxidized co to co2. slight activity was found among species of actinoplanes, agromyces, microbispora, mycobacterium, and other nocardias, and no oxidation was detected in the algae, fungi, and other bacteria tested. carbon monoxide was oxidized rapidly to co2 in the dark in two soils incubated in air or under flooded conditions, but little of the 14c from 14co was incorporated into the organic fraction of these soils. the reaction was microbial because appreciable c ...1979485139
agromyces soli sp. nov., isolated from farm soil.a gram-positive, aerobic to microaerophilic, non-motile bacterial strain, designated mj21(t), was isolated from farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. on the basis of 16s rrna gene sequence analysis, strain mj21(t) was placed within the genus agromyces, and exhibited relatively high levels of similarity to agromyces ulmi xil01(t) (97.8 %), agromyces aurantiacus yim 21741(t) (97.1 %), agromyces mediolanus jcm 3346(t) (96.7 %), a. mediol ...201020639226
the three-dimensional structure of nylon hydrolase and the mechanism of nylon-6 hydrolysis.we performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (nylc) from agromyces sp. at 2.0 å-resolution. this enzyme is a member of the n-terminal nucleophile (n-tn) hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. we observed four identical heterodimers (27kda+9kda), which resulted from the autoprocessing of the precursor protein (36kda) and which constitute the doughnut-shaped quaternary structure. the catalytic resi ...201122187439
agromyces bauzanensis sp. nov., isolated from soil.a gram-stain-positive, aerobic bacterium, designated strain bz41(t), was isolated from hydrocarbon-contaminated soil. phylogenetic analysis based on 16s rrna gene sequences showed that strain bz41(t) was related to members of the genus agromyces and showed highest similarity with the type strain of agromyces ramosus (96.8 %). the morphological, biochemical and chemotaxonomic characteristics of the new isolate were consistent with the description of the genus agromyces. the cell-wall peptidoglyca ...201019933590
Reducing Sugar-Producing Bacteria from Guts of Tenebrio Molitor Linnaeus (Yellow mealworm) for Lignocellulosic Waste Minimization.The guts of Tenebrio Molitor Linnaeus (yellow mealworm) were used as inocula to isolate reducing sugar-producing bacteria during bioconversion of lignocellulose to reducing sugars in this study. Three carbon sources, i.e., carboxymethyl cellulose (CMC), filter paper (FP), and lignocellulosic waste (LIG), were specifically selected; and two types of culturing media (M1 and M2) were used. After 6 months of sequential cultivation, lignocellulose (i.e., polysaccharides) degradation of enrichments M1 ...201121869568
degradation of cyhalofop-butyl (cyb) by pseudomonas azotoformans strain qdz-1 and cloning of a novel gene encoding cyb-hydrolyzing esterase.cyhalofop-butyl (cyb) is a widely used aryloxyphenoxy propanoate (aopp) herbicide for control of grasses in rice fields. five cyb-degrading strains were isolated from rice field soil and identified as agromyces sp., stenotrophomonas sp., aquamicrobium sp., microbacterium sp., and pseudomonas azotoformans ; the results revealed high biodiversity of cyb-degrading bacteria in rice soil. one strain, p. azotoformans qdz-1, degraded 84.5% of 100 mg l(-1) cyb in 5 days of incubation in a flask and util ...201121534595
phylogenetic perspectives of nitrogen-fixing was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ...201121779790
humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost.a bacterial strain isolated from sewage sludge compost, strain sc-083t, was characterized. the isolate was a motile, gram-positive, short rod, forming coryneform v-shaped cells during the early stages of growth. the organism was strictly aerobic and able to grow between 22 and 36 degrees c and between ph 5.5 and 8.0. the predominant fatty acids were cyclohexyl-c17 : 0, anteiso-c17 : 0 and iso-c16 : 0, the major respiratory quinones were menaquinone 11 (mk-11) and 12 (mk-12), and the genomic dna ...200818398211
rare bacterium of new genus isolated with prolonged enrichment culture.dynamic change in microbial flora was monitored with an oxygen electrode. the 1st phase microorganisms, which first grew well in lb medium, were followed by the 2nd phase microorganisms, which supposedly assimilated microbial cells of the 1st phase and their metabolites. in a similar way, a change in microbial flora was observed from the 1st phase to the 4th phase in 84 hr. based on this observation, prolonged enrichment culture was done for as long as two months to increase the ratio of existen ...200414745160
exposure to airborne microorganisms and endotoxin in a potato processing plant.microbiological air sampling was performed in a big potato processing plant located in eastern poland. air samples for determination of concentrations of microorganisms, dust and endotoxin were collected at 6 sites in the division producing potato flakes and meal from dried potato pulp and at 2 sites in the division producing potato syrup from imported starch. the concentrations of total airborne microorganisms were within a range of 28.3-93.1 x 10(3) cfu/m(3). mesophilic bacteria were dominant ...200212498592
agromyces luteolus sp. nov., agromyces rhizospherae sp. nov. and agromyces bracchium sp. nov., from the mangrove rhizosphere.the taxonomic positions of four strains isolated from the mangrove rhizosphere were studied by a polyphasic approach using phenotypic, chemotaxonomic and genetic methods. the four isolates contain 2,4-diaminobutyric acid in their peptidoglycan, and rhamnose as the major cell wall sugar. the predominant menaquinones are mk-12 and mk-11. the predominant cellular fatty acids are iso-c16:0, anteiso-c15:0 and/or anteiso-c17:0. the g+c content of the dna ranges from 70.0 to 73.3 mol%. the four strains ...200111491355
leifsonia poae gen. nov., sp. nov., isolated from nematode galls on poa annua, and reclassification of 'corynebacterium aquaticum' leifson 1962 as leifsonia aquatica (ex leifson 1962) gen. nov., nom. rev., comb. nov. and clavibacter xyli davis et al. 1984 with two subspecies as leifsonia xyli (davis et al. 1984) gen. nov., comb. nov.the new genus leifsonia gen. nov. with two new species, leifsonia poae sp. nov. (type strain vkm ac-1401t) and leifsonia aquatica (ex leifson 1962) nom. rev., comb. nov. (the type species, with vkm ac-1400t = dsm 20146t = jcm 1368t as type strain), is proposed to accommodate bacteria found in poa annua root gall, induced by the nematode subanguina radicicola, and 'corynebacterium aquaticum' leifson 1962. further, it is proposed to reclassify clavibacter xyli davis et al. 1984 with two subspecies ...200010826825
analysis of bacterial dna in skin and muscle of the tyrolean iceman offers new insight into the mummification process.about 80 sequences (16s ribosomal rna gene) of bacterial dna in samples of skin and muscle taken directly from the tyrolean iceman (3350-3100 years b.c.) or recovered during the 1992 archaeological expedition at the alpine site were analyzed to obtain clues to the natural mummification process that allowed the corpse of the neolithic shepherd/hunter to be preserved for more than 5,000 years. the investigation was made more complex by the fact that the surface of the mummy had been swabbed with p ...200010640948
[1,5-poly(ribitol phosphate) with tetrasaccharide substituents in the cell wall of agromyces fucosus ssp. hippuratus].cell walls of agromyces fucosus ssp. hippuratus vkm ac-1352t and vkm ac-1353 contain teichoic acids of identical structure. the polymer is of a 1.5-poly(ribitol phosphate) type. each ribitol phosphate residue is substituted at c-4(2) by tetrasaccharide having the following structure: alpha-l-rhap(1-->3)-alpha-l-rhap-(1-->3)-beta-d-glcpnac-(1-- >2)- alpha-l-rhap-(1-->. the teichoic acid chain is made up by 12-13 repeating units. no structurally identical polymer of the procaryotic cell wall has s ...19947873688
agromyces terreus sp. nov., isolated from soil.a gram-positive, non-motile, cream-coloured, curved or straight rod-shaped bacterial strain, ds-10(t), was isolated from soil of dokdo, korea, and its taxonomic position was investigated by using a polyphasic approach. strain ds-10(t) grew optimally at 25 degrees c and ph 7.0-8.0 in the presence of 0.5-1.0 % (w/v) nacl. strain ds-10(t) had peptidoglycan type b2gamma [d-glu-d-dab (diaminobutyric acid)] and galactose and ribose as whole-cell sugars. strain ds-10(t) contained mk-11, mk-12 and mk-10 ...200818523170
characterization of estrogen-degrading bacteria isolated from an artificial sandy aquifer with ultrafiltered secondary effluent as the medium.this study investigated the aerobic and anoxic biodegradation of four estrogens [estrone (e1), estradiol (e2), estriol (e3), and the synthetic 17 alpha-ethinylestradiol (ee2)] in microcosms constructed with marine sand and ulftrafiltered (uf) secondary effluent. three estrogen-degrading bacteria, lhj1, lhj3, and cyh, were isolated. based on gram-stain morphology and 16s rrna sequence homology, lhj1 and lhj3 belong to the genus acinetobacter and agromyces, respectively; cyh matched to 95% with th ...200717396255
predominant catalase-negative soil bacteria. iii. agromyces, gen. n., microorganisms intermediary to actinomyces and nocardia.the occurrence of filamentous, branching, catalase-negative bacteria as a numerically predominant microflora of various soils was demonstrated by using a dilution frequency isolation procedure. the major characteristics of these organisms were those of the order actinomycetales. however, they could not be placed in any of the present genera of this order and, therefore, a new genus, agromyces, was proposed for these organisms. this genus includes catalase-negative, nutritionally-fastidious micro ...196916349860
agromyces salentinus sp. nov. and agromyces neolithicus sp. nov.a polyphasic study was carried out to clarify the taxonomic position of two gram-positive bacteria isolated from soil samples of the grotta dei cervi (italy), a relatively unexplored hypogean environment. the strains, 20-5t and 23-23t, showed phenotypic and phylogenetic characteristics that were consistent with their classification in the genus agromyces. 16s rrna gene sequence comparisons revealed that the two strains formed distinct phyletic lines within the genus agromyces. based on 16s rrna ...200515653869
agromyces albus sp. nov., isolated from a plant (androsace sp.).agromyces albus sp. nov. is proposed for an aerobic, oxidase- and catalase-positive actinomycete that was isolated from the above-ground part of a plant (androsace sp., in the family primulaceae). the strain is characterized by white colonies, fragmenting hyphae that penetrate into agar media and chemotaxonomic properties that are typical of the genus agromyces: analysis of 16s rdna sequences confirmed that the strain belongs to the genus agromyces and revealed its close phylogenetic relationshi ...200313130029
application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria.strains belonging to the gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (tgge). this method allows the sequence-specific separation of amplified fragments of 16s rrna genes. a total of 115 reference strains representing the majority of the species of the genera aeromicrobium, agromyces, arthrobacter, aureobacterium, cellulomonas, curtobacterium, nocardioides and terrabacter were characterized. depending on the genus investigated, th ...199910028252
taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of clavibacter toxicus as rathayibacter toxicus comb. hplc procedure which separates d- and l-amino acid isomers was applied to an analysis of peptidoglycan of 2,4-diaminobutyric acid (dab)-containing actinomycetes. the cell wall peptidoglycans of species of the genera agromyces, clavibacter and rathayibacter contain dab and have been differentiated principally by their menaquinone profile. these peptidoglycans are known to be identical in structure, all being of the b2 gamma type, possessing both d- and l-dab. the type strains of all the subspe ...19989731278
effects of magnesium, calcium, and serum on reversion of stable l-forms.the l-form of agromyces ramosus was stable in the absence of penicillin when transferred on heart infusion agar containing nacl and serum. it reverted to its bacterial form, however, when magnesium replaced the serum in this medium. on a dilute medium containing nacl but lacking serum, the l-form died out unless calcium, magnesium, or serum was added. it grew as the l-form in the presence of calcium of serum but reverted to the bacterial form in the presence of magnesium. reversion also occurred ...1978711674
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