| the nucleotide sequence of rna3 and rna4 of olive latent virus 2. | olive latent virus 2 (olv2), a virus with particle shapes resembling those of alfalfa mosaic alfamovirus (amv), has four major rna species, two of which (rna3 and rna4) were completely sequenced. rna3 was a bicistronic molecule containing two clear-cut orfs, one of which (orf1) coded for a 36.5 kda polypeptide with conserved motifs of the '30k superfamily' movement proteins and the other (orf2) encoded a 20 kda polypeptide identified as the viral coat protein. rna4, which was a little smaller th ... | 1995 | 9049339 |
| subcellular localization and in vivo identification of the putative movement protein of olive latent virus 2. | the gene encoding the 36.5 kda ('36k') nonstructural protein located on rna3 of olive latent virus 2 (olv-2) was cloned, expressed with the escherichia coli pgex-2t system and the purified protein used to raise a polyclonal antiserum. immunoblot analysis of olv-2-infected nicotiana benthamiana plants showed that the 36k protein accumulated in the early stages of infection and was associated with a subcellular fraction enriched in cytoplasmic membranes. in infected cells there were tubular struct ... | 1999 | 10355755 |
| oleavirus, a new genus in the family bromoviridae. | oleavirus is a monotypic genus having olive latent virus 2 (olv-2) as the type species. olv-2 is transmitted by inoculation of sap but not by aphids. virus particles have different shape and size, ranging from quasi spherical to bacilliform with length of 37, 43, 48, and 55 nm, respectively, and a diameter of ca. 18 nm. virions do not contain lipids or carbohydrates and possess a single coat protein species with molecular mass of ca. 24 kda, which is not required for infectivity. individual part ... | 1997 | 9672653 |
| the nucleotide sequence of rna1 and rna2 of olive latent virus 2 and its relationships in the family bromoviridae. | the complete nucleotide sequence of rna1 and rna2 of olive latent virus 2 (olv-2), a virus with quasi-spherical to bacilliform particles and a non-polyadenylated tripartite ssrna genome, was determined. rna1 consists of 3126 nucleotides and contains a single open reading frame (orf) coding for a polypeptide with a molecular mass of 102689 da (p1a). rna2 is also a monocistronic molecule, 2734 nt in length, coding for a polypeptide with a molecular mass of 90631 da (p2a). the translation products ... | 1996 | 8887501 |