halophage hf2: genome organization and replication strategy. | halophage hf2 is a lytic, broad-host-range bacteriophage of the extremely halophilic domain archaea. it has a 79.7-kb double-stranded dna genome which is linear, contains no modified nucleotides, and is not susceptible to cleavage by many type ii restriction endonucleases. this insensitivity is attributed to selection against palindromic restriction sites, a commonly observed feature of broad-host-range phages. interestingly, enzymes that did cut the genome recognized at-rich sites, and five suc ... | 1995 | 7884878 |
ch2, a novel halophilic archaeon from an australian solar saltern. | a novel halophilic archaeon, strain ch2, was isolated from a marine solar saltern in geelong, australia. the fact that this organism had a dam-methylated genome suggested that it is closely related to the taxon that includes halobacterium saccharovorum, halobacterium sodomense, and halobacterium trapanicum. a sequence analysis of the 16s rrna gene (ch2 has three copies of this gene) showed that ch2 is phylogenetically equidistant from the genera haloarcula and haloferax and closely related to h. ... | 1993 | 8198622 |