| stabilization and particle morphology of prune dwarf virus. | | 1978 | 33484 |
| [about the influence of frost periods upon the serological detection of prunus ring spot viruses in cherries (author's transl)]. | during three years serological tests (latex test) were run from novemeber till april to detect prunus ring spot viruses in forced buds of prunus avium l., p. avium l. var. avium, and p. cerasus l. it was found that prunus necrotic ring spot virus (nrv) could be detected reliably during the winter in all infected trees. in contrary the detection of prune dwarf virus (pdv) was affected by temperatures below zero. in 1971 a low percentage of positive reacting trees was pointed out after the frost p ... | 1977 | 910571 |
| plant virus detection using a new form of indirect elisa. | a novel form of indirect enzyme-linked immunosorbent assay (elisa) has been devised for the detection of viruses in plants. the method uses protein a in two applications to sandwich antibody-antigen-antibody layers. the first applied layer of protein a prepares the plate for the coating antibody layer. the second layer of protein a is conjugated to the enzyme and detects the second antibody layer. the orientation of the igg induced in the coating layer of antibody prevents later unwanted reactio ... | 1985 | 4055975 |
| properties of cherry ring mottle, a distinctive strain of prune dwarf virus. | | 1971 | 5133523 |
| the complete nucleotide sequence of apple mosaic virus rna-3. | the complete nucleotide sequence of apple mosaic ilarvirus (apmv) rna-3 has been determined from cloned viral cdnas. the 5' terminus of rna-3 was determined by direct rna sequencing, while the 3' end was determined by polyadenylation of genomic rna and sub-cloning using oligo dt. apmv rna-3 is 2056 bases in length and encodes at least two open reading frames. it is similar in size and genome organization to the rna-3 of other members of the bromoviridae, which includes ilarviruses. the cp gene i ... | 1995 | 7646355 |
| prunus necrotic ringspot ilarvirus: nucleotide sequence of rna3 and the relationship to other ilarviruses based on coat protein comparison. | the rna3 of prunus necrotic ringspot ilarvirus (pnrsv) has been cloned and its entire sequence determined. the rna3 consists of 1943 nucleotides (nt) and possesses two large open reading frames (orfs) separated by an intergenic region of 74 nt. the 5' proximal orf is 855 nt in length and codes for a protein of molecular mass 31.4 kda which has homologies with the putative movement protein of other members of the bromoviridae. the 3' proximal orf of 675 nt is the cistron for the coat protein (cp) ... | 1995 | 7730792 |
| evolutionary relationships in the ilarviruses: nucleotide sequence of prunus necrotic ringspot virus rna 3. | the complete nucleotide sequence of an isolate of prunus necrotic ringspot virus (pnrsv) rna 3 has been determined. elucidation of the amino acid sequence of the proteins encoded by the two large open reading frames (orfs) allowed us to carry out comparative and phylogenetic studies on the movement (mp) and coat (cp) proteins in the ilarvirus group. amino acid sequence comparison of the mp revealed a highly conserved basic sequence motif with an amphipathic alpha-helical structure preceding the ... | 1997 | 9170502 |
| testing of plum germplasm for sensitivity to plum pox. | a long-term orchard experiment with a broad assortment of plum cultivars aimed to screen their sensitivity to plum pox virus (ppv) was established in 1991. for this purpose, 207 cultivars to be artificially infected with ppv at a permanent site were chosen. the serotype m of ppv from a tree of cv. domestic prune, which had not been contaminated by other viruses, was used as a source of the infection. three buds infected with ppv were budded on 1-year-old trees. in the course of experiment the fo ... | 1998 | 10073233 |
| sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection by rt-pcr. | phenolic compounds from plant tissues inhibit reverse transcription-polymerase chain reaction (rt-pcr). multiple-step protocols using several additives to inhibit polyphenolic compounds during nucleic acid extraction are common, but time consuming and laborious. the current research highlights that the inclusion of 0.65 to 0.70% of sodium sulphite in the extraction buffer minimizes the pigmentation of nucleic acid extracts and improves the rt-pcr detection of potato virus y (pvy) and potato leaf ... | 2002 | 11684310 |
| comparison of elisa and rt-pcr for the detection of prunus necrotic ring spot virus and prune dwarf virus in almond (prunus dulcis). | a technique based on the reverse transcriptase-polymerase chain reaction (rt-pcr) has been developed to detect the presence of prunus necrotic ringspot virus (pnrsv) and prune dwarf virus (pdv) simultaneously in almond. this paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (elisa) and rt-pcr for the detection of pnrsv and pdv using 175 almond leaf samples. multiplex rt-pcr was found to be more sensitive than elisa, especially when followed by nested p ... | 2003 | 14599680 |
| trapping of tomato yellow leaf curl virus (tylcv) and other plant viruses with a groel homologue from the whitefly bemisia tabaci. | to avoid destruction in the haemolymph of their vector, many plant circulative viruses interact with groel homologues produced by insect endosymbiotic bacteria. we have exploited this phenomenon to devise tools allowing trapping of plant viruses by either groel purified from the whitefly bemisia tabaci or by whitefly groel over-expressed in e. coli. pcr tubes or 96-well plates coated with a groel preparation were incubated with cleared sap of virus infected plant leaves or insect vectors. groel- ... | 2004 | 15290375 |
| immunodiagnosis of prune dwarf virus using antiserum produced to its recombinant coat protein. | certification represents the first line of defense against fruit tree viruses. for certification or surveys dealing with large number of samples, elisa is still considered the technique of choice and requires a continuous supply of good quality antibodies. prune dwarf virus (pdv) is among the major viruses affecting stone fruits; it belongs to the genus ilarvirus named so for its isometric labile particles. recombinant dna technology was investigated for production of pdv antiserum to avoid labi ... | 2004 | 15350730 |
| simultaneous detection of six stone fruit viruses by non-isotopic molecular hybridization using a unique riboprobe or 'polyprobe'. | a new strategy for the simultaneous detection of plant viruses by molecular hybridization has been developed. two, four or six viral sequences were fused in tandem and transcribed to render unique riboprobes and designated as 'polyprobes'. the 'polyprobe four' (poly 4) covered the four ilarviruses affecting stone fruit trees including apple mosaic virus (apmv), prunus necrotic ringspot virus (pnrsv), prune dwarf virus (pdv), and american plum line pattern virus (aplpv) whereas the 'polyprobe two ... | 2004 | 15664050 |
| genomic variability of prune dwarf virus as affected by agricultural practice. | twelve new sequences of the coat protein gene of prune dwarf virus (pdv) variants, obtained from almond trees, are presented. comparison with previously reported sequences of the same region, obtained from other hosts (plum, cherry and peach) revealed not only the existence of a wider range of variants of pdv than formerly predicted, but also the frequent presence of a mixture of variants in each sample. in spite of the heterogeneity found in almond, the amino acid composition of the domain at t ... | 2005 | 15789267 |
| new features in the genus ilarvirus revealed by the nucleotide sequence of fragaria chiloensis latent virus. | fragaria chiloensis latent virus (fcllv), a member of the genus ilarvirus was first identified in the early 1990s. double-stranded rna was extracted from fcllv infected plants and cloned. the complete nucleotide sequence of the virus has been elucidated. rna 1 encodes a protein with methyltransferase and helicase enzymatic motifs while rna 2 encodes the viral rna dependent rna polymerase and an orf, that shares no homology with other ilarvirus genes. rna 3 codes for movement and coat proteins an ... | 2005 | 15878214 |
| simultaneous detection of the three ilarviruses affecting stone fruit trees by nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction. | abstract the three most economically damaging ilarviruses affecting stone fruit trees on a worldwide scale are the related prunus necrotic ringspot virus (pnrsv), prune dwarf virus (pdv), and apple mosaic virus (apmv). nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction (rt-pcr) methodologies were developed that could detect all these viruses simultaneously. the latter technique was advantageous because it was discriminatory. for rt-pcr, a degenerate ... | 2000 | 18943373 |
| cellular location of prune dwarf virus in almond sections by in situ reverse transcription-polymerase chain reaction. | abstract in situ reverse transcription-polymerase chain reaction (rt-pcr) was used in young leaves (from trees and in vitro shoots) and flower buds of almond (prunus dulcis), a stone fruit, for cellular location of prune dwarf virus (pdv, a member of the genus ilarvirus). sections obtained from samples fixed in formaldehyde and embedded in paraffin were refixed in formaldehyde to increase tissue preservation in the rt-pcr steps. the coat protein gene of pdv was used as the target to produce a cd ... | 2003 | 18944337 |
| detection of prune dwarf virus by one-step rt-pcr and its quantitation by real-time pcr. | a quantitative pcr, real-time rt-pcr, and one-step real-time rt-pcr using sybr green-based tools for reliable detection and relative quantitation of prune dwarf virus (pdv) in stone fruits are described. the assay reliability was tested on 55 samples from different hosts and regions. the sensitivity of the assay was also compared with other assays with different primers. two plant-expressed genes, actin and 18s rrna, were used as housekeeping genes for accurate quantitation of pdv in stone fruit ... | 2010 | 20003914 |
| ilarviruses of prunus spp.: a continued concern for fruit trees. | prunus spp. are affected by a large number of viruses, causing significant economic losses through either direct or indirect damage, which results in reduced yield and fruit quality. among these viruses, members of the genus ilarvirus (isometric labile ringspot viruses) occupy a significant position due to their distribution worldwide. although symptoms caused by these types of viruses were reported early in the last century, their molecular characterization was not achieved until the 1990s, muc ... | 2012 | 23148725 |
| genetic diversity in the coat protein genes of prune dwarf virus isolates from sweet cherry growing in turkey. | sweet cherry is an important fruit crop with increasing economical value in turkey and the world. a number of viruses cause diseases and economical losses in sweet cherry. prune dwarf virus (pdv), is one of the most common viruses of stone fruits including sweet cherry in the world. in this study, pdv was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of isparta province of turkey by double antibody sandwich-enzyme linked immunosorbent assay (das-elisa) ... | 2015 | 25774109 |
| development and practical use of rt-pcr for seed-transmitted prune dwarf virus in quarantine. | among imported plants, seeds are the items that have many latent pathogens and are difficult to inspect. also, they are the import and export items whose market is expected to expand. the biggest problem with seeds is viruses. prune dwarf virus (pdv) is the virus that is commonly inspected in prunus cerasifera, p. persica, p. armeniaca, p. mandshurica, p. cerasus, p. avium or p. serotina seeds. in this study, two rt-pcr primer sets, which can promptly and specifically diagnose plant quarantine s ... | 2014 | 25289000 |
| a new ilarvirus isolated from viola × wittrockiana and its detection in pansy germoplasm by qrt-pcr. | an infectious agent was transmitted mechanically from samples of viola spp. showing white mosaic and leaf deformation to nicotiana benthamiana. dsrna extracted from the n. benthamiana plants migrated as four specific bands that were absent in non-inoculated plants. sequence analysis of cdna clones generated from the second-smallest dsrna showed the greatest similarity to the rna3 of prune dwarf virus (pdv) (genus ilarvirus, family bromoviridae). however, because of differences in molecular, biol ... | 2014 | 24048886 |
| engineering cherry rootstocks with resistance to prunus necrotic ring spot virus through rnai-mediated silencing. | prunus necrotic ringspot virus (pnrsv) is a major pollen-disseminated ilarvirus that adversely affects many prunus species. in this study, an rna interference (rnai) vector part27-pnrsv containing an inverted repeat (ir) region of pnrsv was transformed into two hybrid (triploid) cherry rootstocks, 'gisela 6' (gi 148-1) and 'gisela 7'(gi 148-8)', which are tolerant and sensitive, respectively, to pnrsv infection. one year after inoculation with pnrsv plus prune dwarf virus, nontransgenic 'gisela ... | 2013 | 23521804 |
| generic amplicon deep sequencing to determine ilarvirus species diversity in australian prunus. | the distribution of ilarvirus species populations amongst 61 australian prunus trees was determined by next generation sequencing (ngs) of amplicons generated using a genus-based generic rt-pcr targeting a conserved region of the ilarvirus rna2 component that encodes the rna dependent rna polymerase (rdrp) gene. presence of ilarvirus sequences in each positive sample was further validated by sanger sequencing of cloned amplicons of regions of each of rna1, rna2 and/or rna3 that were generated by ... | 2017 | 28713347 |