| striking similarities between the nucleotide sequence and genome organization of citrus tatter leaf and apple stem grooving capilloviruses. | the sequence of the 3'-terminal 2956 nucleotides, excluding the poly(a) tail, of the citrus tatter leaf virus (ctlv) genome was determined and compared with that of the apple stem grooving virus (asgv) genome. the sequence of the 3'-terminal region of ctlv contains two overlapping open reading frames (orfs) and a 3'-terminal non-coding region of 142 nucleotides. the long, incomplete orf1 ends at uag (position 2812) and encodes a protein with at least 938 amino acids (m(r) > 108,703). this protei ... | 1993 | 8277280 |
| random mutagenesis in a plant viral genome using a dna repair-deficient mutator escherichia coli strain. | random mutagenesis in a plant viral genome is valuable for generating attenuated strains or for analyzing viral gene function at the molecular level. a dna repair-deficient mutator escherichia coli strain was used for random mutagenesis of a plant viral genome. a full-length infectious cdna clone of citrus tatter leaf virus (genus capillovirus) l strain (ctlv-l) genomic rna under the t7 promoter sequence (pitcl) was introduced into the mutator e. coli strain xl1-red and mutagenized overnight. to ... | 2001 | 11337038 |
| simultaneous detection of six citrus viroids and apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction. | we developed a multiplex reverse transcription polymerase chain reaction (rt-pcr) to detect six citrus viroids: citrus exocortis viroid (cevd), citrus bent leaf viroid (cblvd), hop stunt viroid (hsvd), citrus viroid iii (cvd-iii), citrus viroid iv (cvd-iv) and citrus viroid os (cvd-os) and apple stem grooving virus (asgv, synonym: citrus tatter leaf virus (ctlv)) from citrus plants. the multiplex rt-pcr was also designed to distinguish cvd-i-lss (a distinct variant of cblvd) from cblvd. by the m ... | 2002 | 12393154 |
| nucleotide sequences of a korean isolate of apple stem grooving virus associated with black necrotic leaf spot disease on pear (pyrus pyrifolia). | pear black necrotic leaf spot (pbnls) is a disease of pears caused by capillovirus-like particles, which can be observed under the electron microscope. the disease was analyzed by western blot analysis with antisera raised against apple stem grooving virus (asgv) coat protein. cdnas covering the entire genome were synthesized by rt-pcr and race using rna isolated from chenopodium quinoa infected with sap extracted from pear leaves carrying black necrotic spot disease. the complete genome sequenc ... | 2004 | 15528995 |
| a multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees. | a multiplex polymerase chain reaction (mpcr) assay was developed to detect six rna and one dna citrus virus: citrus leaf rugose virus (clrv), citrus psorosis virus (cpsv), citrus tatter leaf virus (ctlv), citrus tristeza virus (ctv), citrus variegation virus (cvv), citrus yellow mosaic virus (cymv), and indian citrus ringspot virus (icrsv) from citrus plants. these seven viruses are classified in six different virus genera. degenerate primers were designed based on the respective virus isolate s ... | 2005 | 15951030 |
| molecular variability of the genomes of capilloviruses from apple, japanese pear, european pear, and citrus trees. | abstract the 3'-terminal regions of the genomes of apple stem grooving virus (asgv), isolated from apple, japanese pear, and european pear plants, and citrus tatter leaf virus (ctlv), isolated from citrus plants, were amplified by reverse transcription-polymerase chain reaction. the dna products were cloned and sequenced. the results indicated that the asgv isolates from apple, japanese pear, and european pear comprise at least two to four "sequence variants" that differ considerably from each o ... | 1997 | 18945117 |
| molecular characterization of citrus tatter leaf virus historically associated with meyer lemon trees: complete genome sequence and development of biologically active in vitro transcripts. | citrus tatter leaf virus isolated from meyer lemon trees (ctlv-ml) from california and florida induces bud union incompatibility of citrus trees grafted on the widely used trifoliate and trifoliate hybrid rootstocks. the complete genome sequence of ctlv-ml was determined to be 6,495 nucleotides (nts), with two overlapping open reading frames (orfs) and a poly (a) tail at the 3' end. the genome organization is similar to other capilloviruses, with orf1 (nts 37 to 6,354) encoding a putative 242-kd ... | 2009 | 19271984 |
| a real-time rt-qpcr assay for the detection of citrus tatter leaf virus. | citrus tatter leaf virus (cltv) is globally distributed wherever citrus is grown, and, given the extensive use of ctlv sensitive rootstock, has the potential to be a significant threat to the citrus industry. in order to facilitate fast and reliable detection of this virus, we have developed a ctlv-specific real-time rt-qpcr assay. the optimized assay was found to be more reliable and sensitive compared to elisa and end-point rt-pcr, detecting ctlv in up to 70% more plants. the real-time rt-qpcr ... | 2017 | 28274745 |
| integrated analyses using rna-seq data reveal viral genomes, single nucleotide variations, the phylogenetic relationship, and recombination for apple stem grooving virus. | next-generation sequencing (ngs) provides many possibilities for plant virology research. in this study, we performed integrated analyses using plant transcriptome data for plant virus identification using apple stem grooving virus (asgv) as an exemplar virus. we used 15 publicly available transcriptome libraries from three different studies, two mrna-seq studies and a small rna-seq study. | 2016 | 27507588 |
| characterization of the 5'- and 3'-terminal subgenomic rnas produced by a capillovirus: evidence for a cp subgenomic rna. | the members of capillovirus genus encode two overlapping open reading frames (orfs): orf1 encodes a large polyprotein containing the replication-associated proteins plus a coat protein (cp), and orf2 encodes a movement protein (mp), located within orf1 in a different reading frame. organization of the cp sequence as part of the replicase orf is unusual in capilloviruses. in this study, we examined the capillovirus genome expression strategy by characterizing viral rnas produced by citrus tatter ... | 2009 | 19155038 |
| nucleotide sequence of the 3'-terminal region of citrus tatter leaf virus rna. | the 3'-terminal sequence of citrus tatter leaf virus lily isolate (ctlv-l) was determined from cloned cdna. the sequence contains two open reading frames (orfs). orf1 encodes a protein that contains consensus sequences associated with the rna-dependent rna polymerase. orf2, which is in a different reading frame within orf1, can encode a 36 kd protein, putatively identified as a movement protein. ctlv-l coat protein (cp) was found to be located in the c-terminal region of the polyprotein encoded ... | 1994 | 8073638 |