| physical mapping and molecular cloning of mung bean yellow mosaic virus dna. | viral single-stranded dna of mung bean yellow mosaic virus (mymv) was converted to the double-stranded state in vitro, and physical mapping was carried out. the genome of mymv was found to consist of two major components (designated as dna 1 and dna 2). in addition, some minor components were detected. molecular cloning of the major components was carried out, using in vitro double-stranded dna and replicative intermediate dnas. dna 1 is about 2.72 and dna 2 about 2.67 kilobase pairs. no similar ... | 1990 | 2159956 |
| single-stranded dna in mung bean yellow mosaic virus. | | 1985 | 4069018 |
| the nucleotide sequence and genome structure of mung bean yellow mosaic geminivirus. | complete nucleotide sequences of the infectious cloned dna components (dna 1 and dna 2) of mung bean yellow mosaic virus (mymv) were determined. mymv dna 1 and dna 2 consists of 2,723 and 2,675 nucleotides respectively. dna 1 and dna 2 have little sequence similarity except for a region of approximately 200 bases which is almost identical in the two molecules. analysis of open reading frames revealed nine potential coding regions for proteins of mol. wt. > 10,000, six in dna 1 and three in dna 2 ... | 1993 | 8231962 |
| molecular characterization of the rep protein of the blackgram isolate of indian mungbean yellow mosaic virus. | the complete nucleotide sequence of the blackgram isolate of mungbean yellow mosaic virus, imymv-bg, which infects legumes in india, was determined and compared at the amino acid level with those of other whitefly-transmitted geminiviruses. the genome organization of imymv-bg was similar to that of the begomoviruses. a unique feature of the genome organization was the sequence divergence of the common region (cr) between dna-a and dna-b. in order to understand the mechanism of viral dna replicat ... | 2001 | 11562548 |
| the dna-a component of a plant geminivirus (indian mung bean yellow mosaic virus) replicates in budding yeast cells. | understanding the biochemistry of dna replication of the plant dna viruses is important for the development of antiviral strategies. since dna replication is little studied in plants, a genetically tractable, easily culturable, eukaryotic model system is required to pursue such studies in a facile manner. here we report the development of a yeast model system that supports dna replication of a chosen geminivirus strain, indian mung bean yellow mosaic virus. the replication of plasmid dna in the ... | 2004 | 14963136 |
| begomoviruses from mungbeans in pakistan: epitope profiles, dna a sequences and phylogenetic relationships. | monoclonal antibodies raised against particles of african cassava mosaic virus, indian cassava mosaic virus or okra leaf curl virus were used to test samples collected in pakistan from begomovirus-infected plants. epitope profiles obtained from cucurbits resembled those previously reported for pakistani begomoviruses. in contrast, epitope profiles obtained from legumes showed little diversity and were quite distinct from these. dna with nucleotide sequences typical of begomovirus dna a component ... | 2004 | 15045567 |
| analysis of an isolate of mungbean yellow mosaic virus (mymv) with a highly variable dna b component. | one dna a (ka30) and five different dna b components (ka21, ka22, ka27, ka28 and ka34) of a geminivirus, mungbean yellow mosaic virus-vigna (mymv-vig) were cloned from a pooled sample of field-infected vigna mungo plants from vamban, south india. mymv-vig dna a (ka30) and one of the dna b components (ka27) exhibited 97% and 95% sequence identities, respectively, to those of mymv reported from thailand. however, the dna b components ka21, ka22, ka28 and ka34 exhibited only 71 to 72% sequence iden ... | 2004 | 15290387 |
| infectivity analysis of two variable dna b components of mungbean yellow mosaic virus-vigna in vigna mungo and vigna radiata. | mungbean yellow mosaic virus-vigna (mymv-vig), a begomovirus that causes yellow mosaic disease, was cloned from field-infected blackgram (vigna mungo). one dna a clone (ka30) and five different dna b clones (ka21, ka22, ka27, ka28 and ka34) were obtained. the sequence identity in the 150-nt common region (cr) between dna a and dna b was highest (95%) for ka22 dna b and lowest (85.6%) for ka27 dna b. the rep-binding domain had three complete 11-nt (5'-tgtatcggtgt-3') iterons in ka22 dna b (and ka ... | 2004 | 15381851 |
| pcna interacts with indian mung bean yellow mosaic virus rep and downregulates rep activity. | proliferative cell nuclear antigen (pcna), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. the biochemical role of pcna in rolling circle replication (rcr) of geminivirus dna has not been explored in detail. the initiation of rcr of the bipartite genome of a geminivirus, indian mung bean yellow mosaic virus (imymv), is mainly controlled by viral protein rep (or al1 or ac1). the role ... | 2004 | 15479830 |
| molecular characterization of two soybean-infecting begomoviruses from india and evidence for recombination among legume-infecting begomoviruses from south [corrected] south-east asia. | the complete nucleotide sequences of two soybean-infecting begomoviruses have been determined from central and southern parts of india. sequence analyses show that the isolate from central india is a strain of mungbean yellow mosaic india virus (mymiv) and the southern indian isolate is a strain of mungbean yellow mosaic virus (mymv). multiple dna b components could be detected with the soybean strain of mungbean yellow mosaic virus species. the nucleotide sequence similarity between the dna a c ... | 2005 | 15681067 |
| suppression of rna silencing by a geminivirus nuclear protein, ac2, correlates with transactivation of host genes. | bipartite geminiviruses encode a small protein, ac2, that functions as a transactivator of viral transcription and a suppressor of rna silencing. a relationship between these two functions had not been investigated before. we characterized both of these functions for ac2 from mungbean yellow mosaic virus-vigna (mymv). when transiently expressed in plant protoplasts, mymv ac2 strongly transactivated the viral promoter; ac2 was detected in the nucleus, and a split nuclear localization signal (nls) ... | 2005 | 15681452 |
| coat proteins of rice tungro bacilliform virus and mungbean yellow mosaic virus contain multiple nuclear-localization signals and interact with importin alpha. | transport of the viral genome into the nucleus is an obligatory step in the replication cycle of plant pararetro- and geminiviruses. in both these virus types, the multifunctional coat protein (cp) is thought to be involved in this process. here, a green fluorescent protein tagging approach was used to demonstrate nuclear import of the cps of rice tungro bacilliform virus (rtbv) and mungbean yellow mosaic virus--vigna (mymv) in nicotiana plumbaginifolia protoplasts. in both cases, at least two n ... | 2005 | 15914861 |
| promoters, transcripts, and regulatory proteins of mungbean yellow mosaic geminivirus. | geminiviruses package circular single-stranded dna and replicate in the nucleus via a double-stranded intermediate. this intermediate also serves as a template for bidirectional transcription by polymerase ii. here, we map promoters and transcripts and characterize regulatory proteins of mungbean yellow mosaic virus-vigna (mymv), a bipartite geminivirus in the genus begomovirus. the following new features, which might also apply to other begomoviruses, were revealed in mymv. the leftward and rig ... | 2005 | 15956560 |
| factors contributing to deletion within mungbean yellow mosaic virus partial dimers in binary vectors used for agroinoculation. | mungbean yellow mosaic virus-vigna (mymv) sequences cloned as partial dimers within the t-dna of a binary vector were deleted at a high frequency upon conjugal mobilization from escherichia coli into agrobacterium tumefaciens. this deletion involving the genome-length viral dna did not occur when the binary plasmid was inside e. coli and when the binary plasmid was introduced into agrobacterium by electroporation. deletions occurred in both dna a and dna b partial dimers. a minimum of 500-nt con ... | 2006 | 16837070 |
| expression of full-length and truncated rep genes from mungbean yellow mosaic virus-vigna inhibits viral replication in transgenic tobacco. | mungbean yellow mosaic virus-vigna (mymv-vig) is a bipartite geminivirus that causes a severe yellow mosaic disease in blackgram. an assay was developed to study mymv-vig replication by agroinoculation of tobacco leaf discs with partial dimers of the virus. this assay, in a non-host model plant, was used to evaluate pathogen-derived resistance contributed by mymv-vig genes in transgenic plants. viral dna accumulation was optimum in tobacco leaf discs cultured for 10 days after infection with agr ... | 2006 | 16991009 |
| the oligomeric rep protein of mungbean yellow mosaic india virus (mymiv) is a likely replicative helicase. | geminiviruses replicate by rolling circle mode of replication (rcr) and the viral rep protein initiates rcr by the site-specific nicking at a conserved nonamer (taatatt downward arrow ac) sequence. the mechanism of subsequent steps of the replication process, e.g. helicase activity to drive fork-elongation, etc. has largely remained obscure. here we show that rep of a geminivirus, namely, mungbean yellow mosaic india virus (mymiv), acts as a replicative helicase. the rep-helicase, requiring > or ... | 2006 | 17142233 |
| the mungbean yellow mosaic begomovirus transcriptional activator protein transactivates the viral promoter-driven transgene and causes toxicity in transgenic tobacco plants. | the begomovirus transcriptional activator protein (trap/ac2/c2) is a multifunctional protein which activates the viral late gene promoters, suppresses gene silencing, and determines pathogenicity. to study trap-mediated transactivation of a stably integrated gene, we generated transgenic tobacco plants with a mungbean yellow mosaic virus (mymv) av1 late gene promoter-driven reporter gene and supertransformed them with the mymv trap gene driven by a strong 35s promoter. we obtained a single super ... | 2007 | 17990962 |
| cowpea golden mosaic disease in gujarat is caused by a mungbean yellow mosaic india virus isolate with a dna b variant. | it has long been assumed that cowpea golden mosaic disease (cgmd) in southern asia is caused by a begomovirus distinct from those causing disease in other legumes. the components of a begomovirus causing cgmd in western india were isolated, cloned and sequenced. analysis of the sequences shows the virus to be an isolate of mungbean yellow mosaic india virus, but with a distinct dna b component with greater similarity to components of a second legume-infecting begomovirus occurring in the region, ... | 2008 | 18516489 |
| molecular characterisation and infectivity of a "legumovirus" (genus begomovirus: family geminiviridae) infecting the leguminous weed rhynchosia minima in pakistan. | the legume yellow mosaic viruses (lymvs) that cause extensive losses to grain legumes across southern asia are an evolutionarily unusual group of begomoviruses (genus begomovirus; family geminiviridae) with bipartite genomes. all previously identified lymvs were isolated from leguminous crop species. here we have identified a virus related to the lymvs in a common weed, the legume rhynchosia minima originating from pakistan. analysis of the sequence of the virus shows it to be a typical bipartit ... | 2009 | 19664665 |
| selectable marker elimination in the t0 generation by agrobacterium-mediated co-transformation involving mungbean yellow mosaic virus trap as a non-conditional negative selectable marker and bar for transient positive selection. | transient selection involving the bar gene and non-conditional negative selection against stable t-dna integration through the use of the mungbean yellow mosaic virus (mymv) transcriptional activator protein gene (trap) were used in a novel co-transformation strategy to generate selectable marker gene (smg)-eliminated transgenic tobacco plants in the t(0) generation itself. two compatible binary plasmids, pcam-bar-trap-gus harbouring bar as an smg and the mymv trap gene as a non-conditional nega ... | 2010 | 20204372 |
| genetic diversity and phylogeography of begomoviruses infecting legumes in pakistan. | grain legumes are an important source of dietary protein across southern asia, but they suffer extensive losses due to several viruses that are members of the genus begomovirus (family geminiviridae), which are collectively known as legume yellow mosaic viruses (lymvs). despite their economic importance, little attention has been paid to lymvs in pakistan and only partial sequences of virus isolates originating from this country are available in the databases. here, a survey of lymvs occurring a ... | 2010 | 20375225 |
| legume yellow mosaic viruses: genetically isolated begomoviruses. | summary: the yellow mosaic diseases of a number of legumes across southern asia are caused by four species of whitefly-transmitted geminiviruses (genus begomovirus, family geminiviridae): mungbean yellow mosaic virus, mungbean yellow mosaic india virus, dolichos yellow mosaic virus and horsegram yellow mosaic virus. they cause losses to a number of important pulse crops, a major source of dietary protein in the region. the viruses have host ranges limited to plants of the family fabaceae and eff ... | 2007 | 20507504 |
| severe stunting in blackgram caused by the mungbean yellow mosaic virus (mymv) ka27 dna b component is ameliorated by co-infection or post-infection with the ka22 dna b: mymv nuclear shuttle protein is the symptom determinant. | mungbean yellow mosaic virus-[india:vigna] (mymv-[in:vig]), a blackgram isolate of mymv, has five variable and infective dna b components of which ka22 and ka27 dna bs share only 72% nucleotide sequence identity between them. agroinoculation of blackgram with partial dimers of dna a and ka27 dna b caused severe stunting and an inordinate delay in flowering. interestingly, co-agroinoculation of ka27+ka22 dna b components along with dna a ameliorated severe stunting, rescued from the delay in flow ... | 2011 | 21310197 |
| infectivity analysis of a blackgram isolate of mungbean yellow mosaic virus and genetic assortment with mymiv in selective hosts. | yellow mosaic disease in grain legumes in indian subcontinent is caused by two important virus species viz. mungbean yellow mosaic virus (mymv) and mungbean yellow mosaic india virus (mymiv), belonging to the genus begomovirus of the family geminiviridae. the genomic components of a begomovirus causing yellow mosaic disease in blackgram in southern india were cloned and sequenced. nucleotide sequence comparison of dna a component shows the virus isolate to be a variant of mungbean yellow mosaic ... | 2011 | 21380756 |
| antibegomoviral activity of the agrobacterial virulence protein vire2. | mungbean yellow mosaic geminivirus (mymv) causes severe yellow mosaic disease in blackgram, mungbean, frenchbean, pigeonpea, soybean and mothbean. we attempted to induce resistance against this virus using the transcriptional activator protein gene deleted in the c-terminal activation domain (trap-ôêåad) and agrobacterium tumefaciens vire2. mymv is known to replicate in agroinoculated tobacco leaf discs. three transgenic tobacco plants which harboured a truncated mymv transcriptional activator p ... | 2011 | 21842234 |
| Enhanced nicking activity of Rep in presence of pre-coat protein of Mungbean yellow mosaic India virus. | Yellow mosaic disease causes severe yield loss in grain legumes in Indian subcontinent and south east Asia. The disease is caused by two virus species, Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV). They have genome organization typical of Old World begomoviruses, the unique feature being the presence of an open reading frame (ORF) AV2 upstream of coat protein gene. In order to elucidate its function, ORF AV2 of blackgram isolate, Mungbean yellow mosaic India ... | 2011 | 22173984 |
| rapd assisted selection of black gram (vigna mungo l. hepper) towards the development of multiple disease resistant germplasm. | black gram (vigna mungo l. hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. the yield potential of black gram is shallow due to lack of genetic variability and biotic stress susceptibility. core biotic stress factors include mung bean yellow mosaic virus (mymv), urdbean leaf crinkle virus (uclv), wilt (fusarium oxysporum) and powdery mildew (erysiphe polygoni dc). although many studies determine resistant varieties to a parti ... | 2017 | 28389895 |
| detection of quantitative trait loci for mungbean yellow mosaic india virus (mymiv) resistance in mungbean (vigna radiata (l.) wilczek) in india and pakistan. | yellow mosaic disease (ymd) is one of the major diseases affecting mungbean (vigna radiata (l.) wilczek). in this study, we report the mapping of the quantitative trait locus (qtl) for mungbean yellow mosaic india virus (mymiv) resistance in mungbean. an f8 recombinant inbred line (ril) mapping population was generated in thailand from a cross between nm10-12-1 (mymiv resistance) and kps2 (mymiv susceptible). one hundred and twenty-two rils and their parents were evaluated for mymiv resistance i ... | 2013 | 24399908 |
| engineering resistance against mungbean yellow mosaic india virus using antisense rna. | yellow mosaic disease of cultivated legumes in south-east asia, is caused by mungbean yellow mosaic india virus (mymiv) and mungbean yellow mosaic virus (mymv) belonging to the genus begomovirus of the family geminiviridae. efforts to engineer resistance against the genus begomovirus are focused mainly on silencing of complementary-sense virus genes involved in virus replication. here we have targeted a complementary-sense gene (aci) encoding replication initiation protein (rep) to develop resis ... | 2010 | 23637483 |
| yellow mosaic symptom caused by the nuclear shuttle protein gene of mungbean yellow mosaic virus is associated with single-stranded dna accumulation and mesophyll spread of the virus. | mungbean yellow mosaic virus-[india:vigna] (mymv-[in:vig]), a blackgram isolate of mymv, causes yellow mosaic disease in blackgram and mungbean. two variable dna-b components, ka22 and ka27, cause distinct symptoms in blackgram [v. mungo (l.) hepper] with the same dna-a component. ka22 + dna-a-agroinoculated blackgram plants displayed yellow mosaic symptom and accumulated high levels of viral single-stranded (ss) dna. ka27 + dna-a-agroinoculated blackgram plants displayed severe stunting symptom ... | 2017 | 27640432 |
| analyses of mymiv-induced transcriptome in vigna mungo as revealed by next generation sequencing. | mungbean yellow mosaic virus (mymiv) is the viral pathogen that causes yellow mosaic disease to a number of legumes including vigna mungo. vm84 is a recombinant inbred line resistant to mymiv, developed in our laboratory through introgression of resistance trait from v. mungo line vm-1. here we present the quality control passed transcriptome data of mock inoculated (control) and mymiv-infected vm84, those have already been submitted in sequence read archive (srx1032950, srx1082731) of ncbi. qc ... | 2016 | 26981413 |
| seed-borne nature of a begomovirus, mung bean yellow mosaic virus in black gram. | the yellow mosaic viruses (ymv) infecting legumes are considered to be the most devastating begomoviruses as they incite considerable yield loss. the yellow discoloration of pods and seeds of infected plants and symptom emergence in the very first trifoliate leaf of the plants in the field were suggestive that the virus may be seed borne, which was investigated in the present study. the distribution of the virus in various parts of the seeds of black gram (vigna mungo l. hepper) plants naturally ... | 2016 | 26646557 |
| transgenic tobacco plants expressing sirna targeted against the mungbean yellow mosaic virus transcriptional activator protein gene efficiently block the viral dna accumulation. | mungbean yellow mosaic virus (mymv) is a bipartite begomovirus that infects many pulse crops such as blackgram, mungbean, mothbean, frenchbean, and soybean. we tested the efficacy of the transgenically expressed intron-spliced hairpin rna gene of the transcriptional activator protein (hptrap) in reducing mymv dna accumulation. tobacco plants transformed with the mymv hptrap gene accumulated 21-22 nt sirna. leaf discs of the transgenic plants, agroinoculated with the partial dimers of mymv, displ ... | 2015 | 26436122 |
| application of scar markers linked with mungbean yellow mosaic virus disease-resistance gene in pakistan mungbean germplasm. | mungbean yellow mosaic virus disease (mymvd) caused by single-stranded dna begomovirus is the most prominent threat to the mungbean crop in pakistan. mungbean genotypes consisting of 127 varieties/lines were screened for mymvd under natural field conditions. no genotypes were found to be immune or highly resistant against mymvd. genotypes showing resistant and tolerant responses in field screening trials were screened using sequence characterized amplified region (scar) markers linked with the m ... | 2015 | 25867432 |
| evaluation of mungbean genotypes based on yield stability and reaction to mungbean yellow mosaic virus disease. | this work was conducted to identify mungbean genotypes showing yield stability and resistance to mungbean yellow mosaic virus (mymv) disease. sixteen genotypes were evaluated in a randomized complete block design with two replications for two years (2011 and 2012) at three locations (gazipur, ishurdi and madaripur) of the bangladesh agricultural research institute. an analysis of variance exhibited significant effects of genotype (g), environment (e), and genotype × environment (g×e) on grain yi ... | 2014 | 25289012 |
| molecular characterization of distinct ymv (yellow mosaic virus) isolates affecting pulses in india with the aid of coat protein gene as a marker for identification. | the present study was carried out to find out the variations present in different isolates of yellow mosaic virus (ymv) causing yellow mosaic disease of pulses in southern parts of india. the coat protein gene of ymv was amplified using gene specific and deng universal primers with dna isolated from ymv infected samples. further, cloning and dna sequencing of cp gene was carried out. cp gene decrypt sequences revealed that ymv infected samples of black gram, cowpea and green gram were similar to ... | 2014 | 24469717 |
| characterization of betasatellite associated with the yellow mosaic disease of grain legumes in southern india. | yellow mosaic disease caused by mungbean yellow mosaic virus (mymv) belonging to the genus begomovirus (the family geminiviridae) is a major constraint in cultivation of grain legumes in india. the urdbean (vigna mungo (l.) hepper) and mungbean (vigna radiata (l.) r. wilczek) samples affected with yellow mosaic disease exhibits yellow mosaic symptoms along with leaf puckering and leaf distortion in tamil nadu. hence the study was performed to find out if there was any association and influence o ... | 2013 | 24294953 |
| mungbean yellow mosaic virus (mymv) ac4 suppresses post-transcriptional gene silencing and an ac4 hairpin rna gene reduces mymv dna accumulation in transgenic tobacco. | mungbean yellow mosaic virus (mymv) is a legume-infecting geminivirus that causes yellow mosaic disease in blackgram, mungbean, soybean, frenchbean and mothbean. ac4/c4, which is nested completely within the rep gene, is less conserved among geminiviruses. much less is known about its role in viral pathogenesis other than its known role in the suppression of host-mediated gene silencing. transient expression of mymv ac4 by agroinfiltration suppressed post-transcriptional gene silencing in nicoti ... | 2013 | 23417222 |
| the allelic relationship of genes giving resistance to mungbean yellow mosaic virus in blackgram. | the allelic relationship of resistance genes for mymv was studied in blackgram (v. mungo (l.) hepper). the resistant donors to mymv - 'pant u84' and 'upu 2', and their f1, f2 and f3 generations - were inoculated artificially using an insect vector, whitefly (bemisia tabaci genn.). the two recessive genes previously reported for resistance were found to be the same in both donors. | 1986 | 24248192 |
| breeding for resistance to diseases in greengram and blackgram. | this review is given on the origin and interrelationship of blackgram and greengram: the symptoms, mode of transmission, and host range of important diseases, namely: mungbean yellow mosaic virus, leaf crinkle virus, leaf curl virus, mosaic mottle virus, cercospora leaf spot, powdery mildew, root and stem rots, bacterial leaf spot and halo blight. the screening for resistance, sources of resistance, including interspecific hybridization, and induced mutations, as well as the genetics of resistan ... | 1981 | 24276321 |
| inheritance of resistance to yellow mosaic virus in blackgram (vigna mungo (l.) hepper). | the inheritance of resistance to mungbean yellow mosaic virus (mymv) was studied in blackgram (vigna mungo (l.) hepper). the highly resistant donors pant u-84 and upu-2 and a highly susceptible line, ul-2, their f1's, f2's and backcrosses were grown with spreader located every 5 to 6 rows. the resistance was found to be digenic and recessive in all the crosses and free from cytoplasmic effect. | 1980 | 24301097 |