| a reovirus in the brown planthopper, nilaparvata lugens. | a new virus, belonging to the reovirus group, was found in an apparently healthy colony of the brown planthopper, nilaparvata lugens, and was referred to as the nilaparvata lugens reovirus (nlrv). the virus was found in the cytoplasm of the insect cells, sometimes associated with tubular structures, which is one of the characteristic features in tissues infected with reoviruses. the virus was purified by carbon tetrachloride clarification, polyethylene glycol precipitation, differential and cscl ... | 1991 | 1919524 |
| nonpathogenic nilaparvata lugens reovirus is transmitted to the brown planthopper through rice plant. | nilaparvata lugens reovirus (nlrv) was found in a colony of the brown planthopper, n. lugens, an important plant virus vector. unlike with usual phytopathogenic reoviruses, there are no visible symptoms on a rice plant which has been attacked by nlrv-infected planthoppers. manner of transmission, host range, and multiplication of nlrv in rice plant were studied by enzyme-linked immunosorbent assay or polymerase chain reaction using reverse transcriptase. nlrv was transovumly transmitted to about ... | 1995 | 7871743 |
| nucleotide sequence of nilaparvata lugens reovirus genome segment s8 coding for the major outer capsid protein. | the complete nucleotide sequence of genome segment 8 (s8) of nilaparvata lugens reovirus (nlrv) was determined. it consisted of 1802 nucleotides containing a long open reading frame (562 amino acids), which was expressed in escherichia coli as a fusion protein. the expressed s8 product, a 62k protein, was detected by western blotting using igg directed against intact nlrv particles. this result indicates that s8 encodes the major outer capsid protein of nlrv. the protein exhibited 18.6% amino ac ... | 1994 | 7931170 |
| cloning of the nilaparvata lugens reovirus genome: conserved terminal nucleotide sequences and nucleotide sequence of genome segment s10. | the segmented double-stranded rna genome of nilaparvata lugens reovirus (nlrv) was cloned, and the nucleotide sequence of genome segment s10 and terminal nucleotide sequences of the rest of the segments were determined. genome segment s10 of nlrv consisted of 1430 nucleotides with a single open reading frame extending for 1293 nucleotides from nucleotide 46. it encoded a polypeptide of 431 amino acids with an m(r) of 49.4k, which was a non-structural protein. the plus-strand rna of all genome se ... | 1994 | 8113731 |
| complete nucleotide sequence of the nilaparvata lugens reovirus: a putative member of the genus fijivirus. | the nucleotide sequences of all genome segments of the nilaparvata lugens reovirus (nlrv), which is found in the brown planthopper nilaparvata lugens, have been determined and some genes have been assigned to structural and functional proteins. the genome of nlrv consists of 28 699 nucleotides and contains at least 11 large open reading frames (orfs). the genome of nlrv is the largest among viruses of the family reoviridae reported to date. the deduced amino acid sequence of genome segment s1 co ... | 1996 | 8558122 |
| taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome. | sequence determination of full-length cdna clones of genomic segments 7-10 (s7-s10) of oat sterile dwarf fijivirus (osdv) revealed that the 5' and 3' ends of the plus strands of these segments had the same conserved terminal sequences, 5' aacgaaaaa and uuuuuuuaguc 3'. these sequences are similar, but not identical, to the conserved terminal nucleotide sequences of the genomic segments of rice black streaked dwarf fijivirus (rbsdv) and maize rough dwarf fijivirus (mrdv). the coding strands of s7 ... | 1998 | 9634091 |
| rice ragged stunt oryzavirus genome segment s4 could encode an rna dependent rna polymerase and a second protein of unknown function. | the complete nucleotide sequence of genome segment s4 of rice ragged stunt oryzavirus (rrsv, thai-isolate) was determined. the 3823 bp sequence contains two large open reading frames (orfs). orf1, spanning nucleotides 12 to 3776, is capable of encoding a protein of m(r) 141,380 (p4a). the p4a amino acid sequence predicted from the nucleotide sequence contains sequence motifs conserved in rna-dependent rna polymerases (rdrps). when compared for evolutionary relationships with rdrps of other reovi ... | 1998 | 9787664 |
| taxonomic implications for fijiviruses based on the terminal sequences of fiji disease fijivirus. brief report. | the 5' and 3' terminal sequences of the plus strand of fiji disease fijivirus (fdv) segments 2, 3, 9 and 10 possess the conserved terminal sequences, 5'aaguuuuu.....cagcagauguc 3'. the 5' sequence is identical to that of maize rough dwarf fijivirus (mrdv) and rice black-streaked dwarf fijivirus (rbsdv), whereas the fdv 3' sequence shares the consensus, cagcnnnnguc, with mrdv and rbsdv. the fdv terminal sequences, and the amino acid sequences from fdv segment 9, are more closely related to those ... | 1999 | 10603181 |
| molecular characterization of bombyx mori cytoplasmic polyhedrosis virus genome segment 4. | the complete nucleotide sequence of the genome segment 4 (s4) of bombyx mori cytoplasmic polyhedrosis virus (bmcpv) was determined. the 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kda. western blot analysis showed that s4 encodes bmcpv protein vp3, which is one of the outer components of the bmcpv virion. sequence analysis of the deduced amino acid sequence of ... | 2001 | 11134312 |
| molecular characterisation of segments 1 to 6 of rice black-streaked dwarf virus from china provides the complete genome. | the nucleotide sequences of segments s1 to s6 of a chinese isolate of rice black-streaked dwarf virus (rbsdv) were determined. this provides the first complete sequence of a plant pathogenic member of the genus fijivirus. the complete ten-segment genome has 29,141 nucleotides, making it the largest reovirus genome so far reported. each of the segments s1-s6 is predicted to encode a single major protein. protein comparisons indicated that s1 encoded an rna dependent rna polymerase, with similarit ... | 2001 | 11811683 |
| nucleotide sequences of double-stranded rna segments from a hypovirulent strain of the white root rot fungus rosellinia necatrix: possibility of the first member of the reoviridae from fungus. | twelve double-stranded (ds) rna segments were detected from a hypovirulent strain w370 of the white root rot fungus rosellinia necatrix. the estimated molecular weights ranged from 0.41 x 10(6) to 2.95 x 10(6). full length cdna clones for eight segments were obtained. northern blot analysis suggested that each segment was genetically unique. the nucleotide sequences of eight full length dsrna segments were determined. one long open reading frame was found in each segment. conserved sequences at ... | 2002 | 12206302 |
| sequence analysis of genome segments s4 and s8 of mal de río cuarto virus (mrcv): evidence that the virus should be a separate fijivirus species. | this is the first sequence-based characterization of mal de río cuarto virus (mrcv), currently classified as a variant of maize rough dwarf virus (mrdv) and exclusively found in south america. we sequenced and analyzed genome segments s4 and s8. mrcv s4 coded for a putative 131.67 kda protein while mrcv s8 coded for a putative 68.26 kda protein containing an atp/gtp-binding motif. the 5' and 3' ends of mrcv segments, were 5'aaguuuuu3' and 5'cagcunnnguc3', respectively. prediction of secondary st ... | 2002 | 12209310 |
| sequence and phylogenetic analysis of genome segments s1, s2, s3 and s6 of mal de río cuarto virus, a newly accepted fijivirus species. | mal de río cuarto virus (mrcv) is a newly described species of the genus fijivirus, family reoviridae. the nucleotide sequence of four mrcv genome segments was determined. mrcv s1, s2, s3 and s6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kda, respectively. mrcv s1 encodes a basic protein that contains conserved rna-dependent rna polymerase motifs, and is homologous to rice black streaked dwarf virus (rbsdv), fiji disease virus (fdv) and nilaparvata lugens reovirus (nlrv) pol ... | 2003 | 12606083 |
| sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. | the complete nucleotide sequences of 10 genomic segments (s1-s10) from an isolate of rice black-streaked dwarf virus causing rough dwarf disease on maize (rbsdv-hbm) in china were determined, a total of 29,142 base pairs (bp). each segment possessed the genus-specific termini with conserved nucleotide sequences of (+) 5'-aaguuuuu......cagcunnnguc-3' and a perfect or imperfect inverted repeat of seven to eleven nucleotides immediately adjacent to the terminal conserved sequence. while the coding ... | 2003 | 14501194 |