| comparative studies on polyguanylate polymerase and polyadenylate polymerase activities in the dna-dependent rna polymerase i fraction from cauliflower. | the properties of poly(g) polymerase and poly(a) polymerase activities in the dna-dependent rna polymerase nucleosidetriphosphate: rna nucleotidyltransferase ec 2.7.7.6 i fraction from cauliflower (brassica oleracea var. botrytis) were comparatively investigated. the ph optimum, the effect of ionic strength, the effect of substrate concentration on the rate of synthesis, the effect of divalent metal ion concentration, and the time course of synthesis at different temperatures were all different ... | 1976 | 7554 |
| serological cross-reactivity among sporothrix schenckii, ceratocystis, europhium, and graphium species. | ethanol-precipitable culture filtrate antigens of 100 strains of 75 species of the sporothrix-ceratocystis-europhium-graphium complex and 1 species of botrytis were examined for neutral sugar components and for serological cross-reactivity with s. schenckii rabbit antiserum and human sporotrichosis sera by capillary precipitin and double immunodiffusion assay. results revealed that cross-reactive species (60 of 77, ca. 80%) produced exoconidial forms and rhamnose- and mannose-containing polysacc ... | 1978 | 99369 |
| [properties of the laccase of botrytis cinerea]. | laccase from botrytis cinerea, secreted by the fungus into the grape berry, is responsible for the oxidation phynomena in musts and wines (oxidasic casse). from a fungus culture, the enzyme was extracted for a study of its properties: specificity; influence of inhibitors, ph and temperature; the results confirm that the enzyme from botrytis cinerea belongs to the laccase family, though it has some characteristic differences from the other laccases previously described. | 1975 | 238748 |
| [fungal spore content of the air in munich (author's transl)]. | a volumetric determination of seasonal variations in the fungal spore content of the air was carried out with andersen samplers (culture plates) in the center of the city of munich and 2.4 km (1.5 miles) west of it. cladosporium spores were predominant (60% altogether), followed by those of penicillium and aspergillus. up to 22 different genera were counted among the varieties. the spores of cladosporium, penicillium, aspergillus, botrytis, thysanophora and epicoccum are twice as densely represe ... | 1977 | 403426 |
| herbicidins f and g, two new nucleoside antibiotics. | a mutant of streptomyces saganonensis no. 4075, obtained with n-methyl-n'-nitro-n-nitrosoguanidine treatment, produced herbicidins f and g without herbicidins a and b. isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous meoh. herbicidin f was obtained as colorless needles after extraction of the eluate using methylene dichloride. purification of herbicidin g was completed with silica-gel chromatography to give a crystalline powder. phys ... | 1979 | 511776 |
| [purfication and molecular weight of an rna-dependant rna polymerase from brassicae oleracea var. botrytis]. | an rna-dependent rna polymerase has been completely purified from cauliflower inflorescences. analysis of the purified enzyme on sds-polyacrylamide gels showed one polypeptide chain with a molecular weight of 140,000 dalton. the enzyme is monomeric in its native state. the in vitro activity was completely dependent on added rna, the most efficient templates being poly (u), poly (u, c), poly (i) and viral rna. | 1978 | 105817 |
| stimulation of de novo synthesis of l-phenylalanine ammonia-lyase in relation to phytoalexin accumulation in colletotrichum lindemuthianum elicitor-treated cell suspension cultures of french bean (phaseolus vulgaris). | (1) the regulation of the accumulation of the isoflavonoid-derived phytoalexin phaseollin in cell suspension cultures of dwarf french bean (phaseolus vulgaris/ has been investigated. (2) an elicitor preparation from cell walls of colletotrichum lindemuthianum, the causal agent of anthracnose disease of french bean, caused a marked accumulation of phaseollin in the cultures. the elicitor induced phaseollin accumulation to a level of 60% that obtained with the artificial elicitor autoclaved ribonu ... | 1979 | 476149 |
| large-scale purification and subunit structure of dna-dependent rna polymerase ii from cauliflower inflorescence. | dna-dependent rna polymerase ii (nucleosidetriphosphate:rna nucleotidyltransferase, ec 2.7.7.6) from cauliflower inflorescence (brassica oleracae, var. botrytis) was highly purified by polyethyleneimine treatment on a large scale. the solubilized enzyme was partially purified by polyethyleneimine fractionation and subjected to chromatography on deae-sephadex and phosphocellulose, and subsequently to sedimentation in a glycerol gradient. the specific activity (231 nmol/mg per 10 min) of this enzy ... | 1978 | 623757 |
| polygalacturonase of botrytis cinerea e-200 pers. | a polygalacturonase (poly(1,4-alpha-d-galacturonide)glycanohydrolase, ec 3.2.1.15) was purified from the culture fluid of botrytis cinerea. the polygalacturonase preparation, homogeneous on the basis of disc-gel electrophoresis also showed pectinesterase activity. some properties of the purified polygalacturonase were studied. it had a molecular weight about 69 000. it was inactivated by p-chloromercuribenzoate, tetranitromethane and urea. a 50% loss in viscosity of sodium polypectate solution o ... | 1975 | 804319 |
| plant pyruvate dehydrogenase complex purification, characterization and regulation by metabolites and phosphorylation. | the pyruvate dehydrogenase complex was purified from mitochondria of cauliflower, brassica oleracea var. botrytis floral buds to a specific activity of 5.4 mumol of nadh/min per mg of protein. the pyruvate dehydrogenase complex required coash, nad+, thiamine pyrophosphate and mg2+ for the oxidative decarboxylation of pyruvate. the kinetic analysis of the complex gave a series of parallel lines for all substrates. product interaction patterns showed that nadh is competitive with nad+; acetyl-coa ... | 1977 | 922017 |
| bovine nasal granuloma in victoria -- a search for the causative allergens. | cows with nasal granuloma showed skin sensitivity to a range of environmental allergens while clinically normal control animals showed little sensitivity. the allergens producing the greatest number of positive reactions included the pollens of dock, capeweed, clover, paspalum, sorrel, rye grass, pepper tree and wattle, the spores of penicillium, cladosporium, botrytis and rye grass rust and extracts of sheep's wool and mite. it is likely that different allergens are important in different anima ... | 1976 | 938353 |
| the degradation of onion cell walls by extracellular enzymes of botrytis allii and sclerotium cepivorum [proceedings]. | | 1978 | 565727 |
| antibiotic-induced changes of mycelial growth of botrytis cinerea. | the effect of antibiotics and metabolic inhibitors on mycelial growth of botrytis cinera was followed. inhibitors of protein synthesis, chloramphenicol, erythromycin and tetracycline inhibit growth or sporulation of botrytis cinera. ethidium bromide, 5-fluorouracil, phenylethylalcohol and k 20 cause granulation, vacuolization and undulation of hyphase. 2,4-dinitrophenol, boromycin, macrotetrolides, monensin, scopathricin and tx2 at subfungistatic concentrations induce intensive branching of hyph ... | 1976 | 987979 |
| metabolism of the phytoalexin rishitin by botrytis spp. | | 1976 | 988112 |
| incidence of fungal spores at the homes of allergic patients in an agricultural community. iii. associations with local crops. | a predominantly agricultural community in california was surveyed for prevalent fungal spores during a 12-month period. alternaria, macrosporium and stemphylium were recovered during asparagus and strawberry harvesting times year-round. fusarium and botrytis were less frequently associated with the strawberry harvest and were recovered only during the first quarter of the year. epicoccum was recovered in the north end of the salinas valley in low numbers throughout the year and was strongly asso ... | 1979 | 574745 |
| microbial transformations of natural antitumor agents. 9. o-demethylation of 9-methoxyellipticine. | microbial transformation studies were conducted with 9-methoxyellipticine (1). several microorganisms including botrytis allii (nrrl 2502), cunninghamella echinulata (nrrl 1386), c. echinulata (nrrl 3655), and penicillium brevicompactum (atcc 10418) achieved o-demethylation of (1) in good yield. p. brevicompactum was used to prepare 9-hydroxyellipticine (4) from 1 for isolation and complete identification. high-performance liquid chromatography was used to verify the identity of the major metabo ... | 1979 | 575547 |
| light-induced synchronous conidiation in the fungus botrytis cinerea. | botrytis cinerea pers. ex fr. in stationary liquid cultures conidiated asynchronously in darkness after 4 days' growth. synchronous conidiation was induced by irradiating dark-grown cultures with near-ultraviolet light for 12 h. the number of conidia increased very rapidly 10 h after the end of the photo-induction period, and conidiation was completed by the 14th hour. filter paper cultures of the fungus also showed synchronous conidiation upon irradiation with near-ultraviolet light, but the ra ... | 1976 | 945324 |
| sensitivity distribution of phytopathogenic bacteria and fungi to antibiotics. | the minimal inhibitory concentrations (mic) of various antibiotics and fungicides for erwinia carotovora, pseudomonas coronafaciens var. atropurpurea, p. lachrymans, alternaria mali, a. kikuchiana, pyricularia oryzae, botrytis sp. and sclerotinia sp. isolated from diseased plants in various localities of japan were examined to enable the isolates to be gruoped into sensitive and resistant strains. to minimize the effects of various variable conditions, mic of isolates were pooled for either 2 or ... | 1976 | 1033174 |
| [new analytic criteria for the characterization of wines]. | isotopic analysis, determination of radioactivity are factors allowing a better characterization of wines. the analysis of the composition in stable isotopes of the constituants of wine can provide new analytic criteria for characterizing wines. thus, the concentration of 13c in ethanol can allow the disclosure of an addition of cane sugar or maize sugar to the musts. a study of the composition in stable isotopes of the water of wines of a certain region made it possible to show a correlation be ... | 1978 | 573085 |
| role of glycosidases in the membranlytic, antifungal action of saponins. | in studies on the membranlytic action of various saponins on mycelium of botrytis cinerea and rhizoctonia solani digitonin, alpha-hederin and tomatin caused considerable leakage of free amino acids, while aescin and theasaponin were less effective. cyclamin significantly damaged cell membranes of r. solani, but did not change the selective permeability of b. cinerea. cell membrane disruption was accompanied by an enzymatic conversion of saponins into their corresponding aglycones in cell membran ... | 1975 | 1171670 |
| the effect of volatile and gaseous metabolites of swelling seeds on germination of fungal spores. | effects of volatile and gaseous metabolites of swelling seeds of pea, bean, wheat, corn cucumber, tomato, lentil, carrot, red papper and lettuce on germination of spores of five genera of fungi were found to depend rather on the fungal than on the plant genus. germination of spores of botrytis cinerea, mucor racemosus and trichoderma viride was most severely inhibited. spores of verticillium dahliae were less sensitive and germination of spores of fusarium oxysporum was inhibited only in two cas ... | 1975 | 1176038 |
| transformation of cauliflower (brassica oleracea l. var. botrytis)--an experimental survey. | the paper compares different approaches for the genetic transformation of cauliflower (agrobacterium-mediated, peg-mediated and/or electroporation). transient expression of the neomycin phosphotransferase ii (nptii) gene could be detected after direct gene transfer. stable transformation was achieved using both agrobacterium-mediated and direct gene transfer. expression as well as incorporation of the nptii sequence could be demonstrated. | 1992 | 1320426 |
| pyrazolo[3,4-d][1,2,3]triazole-1-carboxamides and 5-alkylaminopyrazolo[3,4-d]oxazoles: synthesis and evaluation of the in vitro antifungal activity. | a series of n-alkyl-n'-(4-diazo-5-pyrazolyl)-ureas (4) was thermally and photochemically converted into pyrazolo [3,4-d][1,2,3]triazole derivatives (5,6) and 5-alkylaminopyrazolo[3,4-d]oxazoles (7) respectively. the products were tested for in vitro antifungal activity against fusarium culmorum, botrytis cinerea, phoma betae, pythium ultimum, sclerotinia minor and rhizoctonia solani. the mic and ed50 values of compound (6) against some of the test fungi were comparable to those of the reference ... | 1992 | 1445610 |
| morphogenic effects of ramihyphin a in filamentous fungi. | ramihyphin a at subfungistatic concentrations stimulates ramification of hyphae of filamentous fungi. stimulation of terminal ramification of hyphae that can be observed particularly in phytopathogenic fungi is most frequent. hyphae of microsporon canis, trichophyton mentagrophytes, blastomyces dermatitidis, coccidioides immitis and histoplasma capsulatum ramify intensively laterally. stimulation of the lateral ramification was observed in monilia fructigena, penicillium marneffei and penicilliu ... | 1975 | 1176042 |
| locus of blue and near ultraviolet reversible photoreaction in the stages of conidial development in botrytis cinerea. | the effect of the blue and near ultraviolet reversible photoreaction on conidial development in botrytis cinerea was studied by observing microscopically selected conidiophores. conidiophore development was divided into six stages; when the developing conidiophores from stage 2 (i.e. a mature conidiophore) to stage 5 (i.e. a conidiophore with conidium initial) were exposed to blue light for a short time, conidiation was suppressed; the conidiophores already formed de-differentiated to 'sterile' ... | 1977 | 556757 |
| infection and pathogenesis of cash crops by botrytis cinerea: primary role of an aspartic proteinase. | | 1991 | 1812708 |
| the fungal flora of loganberries in relation to storage and spoilage. | botrytis cinerea, cladosporium spp., penicillium spp., aureobasidium pullulans, mucor mucedo, phoma state of didymella applanata, cryptococcus laurentii var. laurentii, c. albidus var. albidus, candida sake and sporobolomyces roseus were consistently present on freshly harvested loganberries. botrytis cinerea was the main spoilage organism with mucor mucedo of minor importance. the importance of benomyl-resistant strains of b. cinerea is discussed. | 1977 | 557301 |
| observations on the ultrastructure of the choanoflagellate codosiga botrytis (ehr.) saville-kent with special reference to the flagellar apparatus. | the ultrastructure of the choanoflagellate codosiga botrytis is described with particular reference to the flagellar appendages, the flagellar rootlet system, the transition zone, the basal body and accessory centrioles, and the stalk. the controversial early reports of flagellar appendages in this species have been confirmed and they have been detected in 2 further species, salpingoeca frequentissima and monosiga sp. the appendages consist of a delicate bilateral vane 2 mum wide on either side ... | 1975 | 1089676 |
| pine stilbene synthase cdna, a tool for probing environmental stress. | stilbene synthase cdnas were isolated from a pine (pinus sylvestris) cdna library. poly(a)+rna required for the preparation was obtained from young seedlings challenged with botrytis cinerea. a full-length cdna encoding pinosylvin-forming stilbene synthase was sequenced, and the deduced amino acid sequence was compared with sequences of resveratrol-forming stilbene synthases. the cdna coding for the key enzyme of pinosylvin formation is a valuable tool for detecting early effects of environmenta ... | 1992 | 1451785 |
| isolation and characterization of phosmidosine. a new antifungal nucleotide antibiotic. | a new nucleotide antibiotic, phosmidosine was isolated from a culture filtrate of a newly isolated streptomycete identified as streptomyces sp. rk-16. hrfab-ms and elemental analysis established the molecular formula of c16h24n7o8p. 1h, 13c and 31p nmr indicated the presence of a methyl phosphate group and uv spectra were similar to those of 8-hydroxyadenosine. the antibiotic inhibited spore formation of botrytis cinerea at the concentration of 0.25 micrograms/ml. | 1991 | 2032945 |
| use of the extended phadebas rast panel in the diagnosis of mould allergy in asthmatic children. | the clinical significance of mould allergens in phadebas rast panel was investigated in 121 asthmatic children. they were selected from a total population of 1649 patients. the patients were distributed into four groups, based on the combination of positive or negative skin prick tests (spt) together with symptoms suggestive or not of mould sensitivity. mould-specific ige antibodies were investigated using the original rast panel (alternaria, aspergillus, candida, cladosporium, mucor and penicil ... | 1991 | 2039083 |
| [the viability of fungal species from the genera penicillium and botrytis in sea and river water contaminated by liquid swine manure]. | the growth ability of certain types of fungi penicillium and botrytis has been studied in marine and river water, contaminated by liquid swine manure (lsm). it has been found out, that addition of lsm stops p. lilacinum and b. bassiana growth. while the growth of the other types of fungi p. p. penicillium and botrytis, that were studied in the marine and river water, contaminated by lsm, may continue provided the temperature is favourable. the growth is discontinued at water temperature 4-6 degr ... | 1990 | 2074018 |
| [research of antifungal substances secreted by higher fungi in culture]. | a screening in vitro of antifungic activity of 24 strains of basidiomycetes was realized with their culture filtrate. lycoperdon perlatum pers. = pers., oudemansiella platyphylla (pers. ex fr.) mos., agrocybe dura (bolt) singer have shown an activity against candida albicans, candida tropicalis and aspergillus fumigatus; pholiota spumosa (fr.) singer towards botrytis cinerea and lycoperdon perlatum pers. = pers. towards alternaria solani, botrytis cinerea and verticillium dahliae. more extensive ... | 1990 | 2082797 |
| biological studies on 2,1-benzisothiazole derivatives. i. evaluation of antibacterial, antifungal and genotoxic activities of 1-acyl-3-acylimino-2,1-benzisothiazolines. | the in vitro evaluation of antibacterial, antifungal and genotoxic activities of some 1-acyl-3-acylimino-2,1-benzisothiazolines is described. compounds 55, 58, 60 and 62 exhibited a selective antibacterial activity. compounds 1, 2, 30, 59 and 63 were active against botrytis cinerea, compounds 6, 20, 39 and 54 against pythium irregulare. dna-damaging activity in the bacillus subtilis rec-assay was demonstrated for compounds 45, 46, 54, 55, 57 and 61. by the salmonella-microsome assay, genotoxic a ... | 1990 | 2128452 |
| mycoflora and nutritional value of shelled melon seeds (citrulus vulgaris schrad.) in nigeria. | thirteen fungi were isolated from mouldy shelled melon seeds. the fungi more frequently isolated included species of mucor (11.25%), rhizopus (13.75%), aspergillus (36.25%), macrophoma (2.50%), penicillium (8.75%), alternaria (5.00%), fusarium (8.75%), botrytis (6.25%), torula (3.75%) and geotrichum (3.75%). healthy, shelled melon seeds inoculated with fusarium solani for 7 or 14 days caused increases in the free fatty acid (f.f.a.) content of the healthy seeds from an initial value of 1.06% to ... | 1990 | 2170965 |
| study of carbendazin residue accumulation on greenhouse and field-grown strawberries, after successive treatments with benomyl. | benomyl, a systemic fungicide used in preharvest treatments to prevent botrytis and other fungal diseases, metabolizes to carbendazim (mbc). a study was undertaken to determine if the total residues of mbc of greenhouse and field-grown douglas strawberries accumulate in fruits after successive fortnightly treatments with a commercial preparation of benomyl. statistical analysis of the data obtained indicates that residues of mbc do not accumulate in fruits; on the contrary, they decrease over ti ... | 1990 | 2211476 |
| isolation and characterization of three chitinases from trichoderma harzianum. | three proteins which display chitinase activity were purified from the supernatants of trichoderma harzianum cect 2413 grown in minimal medium supplemented with chitin as the sole carbon source. purification was carried out after protein precipitation with ammonium sulphate, adsorption to colloidal chitin and digestion, and, finally, chromatofocusing. by this procedure, two chitinases of 42 kda (chit42) and 37 kda (chit37) were purified to homogeneity, as judged by sds/page and gel filtration, w ... | 1992 | 1606968 |
| behaviour studies of the fungicide cymoxanil in two strains of the fungus botrytis cinerea and in haemolymph of locust and lobster. i. in situ monitoring by internal surface reversed-phase high-performance liquid chromatography. | a method for following the metabolism of the fungicide cymoxanil in various biological media is described. by using a recently developed high-performance liquid chromatographic method, with an internal surface reversed-phase column, it is unnecessary to clean up the sample before analysis. thus this technique makes monitoring in fungi as well as in arthropod haemolymph easier and faster. | 1991 | 1939461 |
| significance of sampling height of airborne particles for aerobiological information. | pollen and spore counts from burkard traps for routine pollen and spore sampling placed at 15 m above ground and at ground level were compared. daily counts of most pollen types were higher on the ground than at roof level, but the counts were significantly correlated. the ratios of pollen frequencies at high and low levels varied between 1.0 and 11.5. the most prominent differences were recorded for herbaceous pollen (e.g. artemisia counts 11.5 and poaccae counts 4.4 times higher at ground leve ... | 1991 | 2018211 |
| fungi carried from farmers' work into farm homes. | airborne fungal spore concentrations and main fungal genera were compared in rural and urban living environments in finland during the winter. in addition to conventional viable fungal spore counts (based on the six-stage impactor sampling and cultivation), total spore concentrations were obtained by scanning electron microscope (sem) investigation of filter samples. the viable spore counts were only 0.2%-25% of the number of total spore aggregates. a high correlation between these two methods w ... | 1989 | 2596402 |
| specific ige antibodies to 16 widespread mold genera in patients with suspected mold allergy. | a panel of 55 sera from patients with suspected mold allergy from two different geographic areas, a and b, was investigated for specific ige antibodies to 16 individual molds using phadebas rast technique. the molds investigated were alternaria, cladosporium, aspergillus, penicillium, mucor, and candida from the former rast panel and ten additional new mold genera. the rast screening revealed that 73% of patients had specific ige antibodies to at least one of the 16 molds. eighteen percent were ... | 1989 | 2596762 |
| purification of polygalacturonases produced by botrytis cinerea e-200. | | 1975 | 1240016 |
| compatibility of the two-site exchange model and 1h nmr relaxation rates. | we demonstrate a formalism for determining whether 1h longitudinal and transverse magnetic relaxation decay curves are compatible with a model of proton exchange between two sites with inherently different relaxation rates. we apply the method to published data in which both decay curves have been fit to normalized biexponential functions, a prediction of the simplest two-site exchange model. the analysis is applied to relaxation data of adsorbed water vapor on silica gel (j.r. zimmerman and j.a ... | 1990 | 2345517 |
| concentrations of airborne botrytis conidia, and frequency of allergic sensitization to botrytis extract. | | 1987 | 2958317 |
| [results of an airborne spore study in various regions of southern sardinia]. | fungal air spores can play a significant role in several allergic manifestations. therefore, the identification of geographic areas of mould distribution could be helpful to the clinician, especially if associated with fungal air spore recording in homes or working environments of sensitized subjects, in determining the real clinical importance of sensitization to fungi. on this basis, we studied the occurrence of airborne fungi at two urban sites and at two rural sites in the south of sardinia, ... | 1989 | 2484495 |
| ["berry sorter's lung" or wine grower's lung--an exogenous allergic alveolitis caused by botrytis cinerea spores]. | | 1987 | 3299353 |
| fungal air-borne spores as health risk factors among workers in alimentary industries. | a survey to evaluate the occurrence of air-borne fungal spores in two different food industries, dairies and bakeries, was conducted. our data revealed considerable fungal pollution in the environments of both industries, as well as some differences in the distribution of the genera of fungi recovered. noteworthy was the frequent finding of numerous fungi frequently responsible for allergic rhinitis, asthma and other diseases, or well-known for their production of mycotoxins in foods or characte ... | 1989 | 2767234 |
| studies on new phosphate ester antifungal antibiotics phoslactomycins. i. taxonomy, fermentation, purification and biological activities. | new antibiotics phoslactomycins a, b, c, d, e and f, which contain alpha, beta-unsaturated delta-lactone, phosphate ester, conjugated diene and cyclohexane ring moieties, were isolated from the culture broth of a soil isolate actinomycete. morphological, cultural and physiological studies revealed that the isolate is a strain of streptomyces nigrescens. phoslactomycins were obtained by butanol extraction, gel filtration and reverse phase chromatography. the antibiotics show strong activity again ... | 1989 | 2753808 |
| volumetric aerobiological survey of conidial fungi in the north-east netherlands. i. seasonal patterns and the influence of metereological variables. | in order to obtain actual data about the qualitative and quantitative occurrence of airborne fungi in the netherlands, a viable-volumetric sample survey with the andersen sampler was undertaken. from april 1981 to the end of june 1983, one day a week samples were taken on the unobstructed rooftop of a hospital in the n-e netherlands. an average of 268 colony forming units (cfu), per sample, per cubic metre of air were found. almost three-quarters of the total catch consisted of seven genera, cla ... | 1985 | 3887975 |
| botrytis cinerea: a study of the immunological properties during growth. incidence of antibodies against b. cinerea in a group of patients with aspergillosis. | when growing botrytis cinerea in sabouraud medium, different phases of growth could be recognized using ph, mycelium yield, and culture filtrate antigens as parameters of growth. immunological characteristics of the different phases (early, intermediate, and late) of growth were measured using ige and igg binding by enzyme allergosorbent test and enzyme-linked immunosorbent assay, respectively, and quantities of precipitating components (double immunodiffusion and rocket immunoelectrophoresis). ... | 1987 | 3112026 |
| human serum antibodies to extracellular polysaccharides (eps) of moulds. | the presence of antibodies to extracellular polysaccharides (eps) of moulds in sera of healthy subjects (n = 125) was determined. antibodies against the eps of penicillium digitatum, mucor racemosus, cladosporium cladosporioides, fusarium moniliforme and botrytis tulipae were found in relatively high amounts in all sera. no effect of age on antibodies present could be demonstrated. antibodies against each of the eps tested were only neutralized by the homologous eps and by eps of moulds belongin ... | 1988 | 3379539 |
| [determination of specific ige against 16 widespread mould genera. improvement of the efficacy of the diagnosis of allergy to moulds]. | serum samples from 55 scandinavian and us patients with a clinical history of mould allergy were screened for specific ige ab against 16 different moulds, the 6 mould in the phadebas rast panel and 10 new moulds. the studies were performed using rast-based techniques and the nitrocellulose immunoblotting method. the rast screening of the patient panel, revealed that 42 patients (76%) had specific ige to at least one of the 16 moulds. the two most frequent moulds were rhizopus and botrytis positi ... | 1987 | 3453126 |
| [isolation and characterization of colloidal soluble polysaccharides in raspberry juice]. | depending on the preparation method, the colloid content of raspberry juices treated with pectolytic enzymes, can be up to 2.5 g/l. analysis of the polysaccharides shows that there are in particular, arabinans, arabinogalactans type ii and rhamnogalacturonans. while the content of arabinans could be reduced by the action of arabinases, the arabinogalactans and rhamnogalacturonans were not degraded by conventional pectic enzymes. besides these cell-wall polysaccharides, it was possible to isolate ... | 1991 | 1927051 |
| characterization of polygalacturonases of botrytis cinerea e-200. | | 1975 | 1240017 |
| conidia of botrytis cinerea: labeling by fluorescent vital staining. | as a model system, conidia of botrytis cinerea have been vitally stained with optical brighteners to facilitate their location in plant tissue. | 1966 | 4159926 |
| cytochemical evidence for the existence of peroxisomes in botrytis cinerea. | | 1969 | 4186256 |
| sequential sampling of fungal air spores inside and outside the homes of mould-sensitive, asthmatic patients: a search for a relationship to obstructive reactions. | a sequential sampling study of fungal airspores was carried out with the andersen sampler inside and outside the homes of eight asthmatic, mould-sensitive patients. the aim of the study was to find a possible relationship between variations in airborne colony forming units (cfu) and the occurrence of increased pulmonary complaints (ipc). great variations in fungal prevalence occurred throughout the year, the lowest average daily concentration being 125, and the highest, 1425 cfu/m3/sample during ... | 1985 | 4061983 |
| [search for actinomycetes--antagonists of fungi causing sugar beet root rot]. | actinomycetes belonging to streptomyces were isolated from the rhizosphere of sugar beet grown on an infected area after cultivation for many years. 44.1 per cent of them proved to be antagonists of phytopathogenic test fungi. the majority of the antagonists were detected among the cinereus. the lowest number of the antagonists was detected among the azureus, the maximum number of the antagonists was observed in the middle and at the end of the sugar beet vegetation period. during various period ... | 1989 | 2534467 |
| mould spores and yeasts in outdoor air. | most inviable spore counts of outdoor air in europe are based on burkard spore trap results. for viable counts different kinds of slit samplers and andersen sampler are used. results of viable and inviable sampling methods are not completely comparable, but the results complete each other. spore frequencies in outdoor air are almost equal in different nordic countries. cladosporium is the most important spore type everywhere. in south finland it accounts for ca. 85% of viable and ca. 50% inviabl ... | 1985 | 4083413 |
| extracellular proteinases of the isolate of botrytis cinerea virulent to apple tissues. | b. cinerea produces extracellular acid proteinases: aspartic proteinase and carboxypeptidase, separable on cm-sepharose cl-6b. aspartic proteinase showed the maximum activity at ph 2.5-3.0, was inactivated by diazoacetyl-dl-norleucine methyl ester and was unable to hydrolyse carbobenzoxy glu-tyr. carboxypeptidase showed the maximum activity at ph 4.7-5.0, was inactivated by diisopropyl fluorophosphate, and carbobenzoxy-glu-tyr served as an efficient enzyme substrate. the isolated aspartic protei ... | 1985 | 3898682 |
| [study of the kinetics and mechanisms of inactivation of the enzymes of the fungus botrytis cinerea pers with uv-rays]. | | 1970 | 4992016 |
| growth and sporulation of botrytis convoluta with various carbon and nitrogen sources. | | 1972 | 5065013 |
| growth of helminthosporia on media containing the fungicide benomyl and the assay of benomyl using botrytis cinerea. | | 1973 | 4735621 |
| plate assay for determining the time of production of protease, cellulase, and pectinases by germinating fungal spores. | a new method for detecting enzymes produced by fungal spores during germination is described here. with this method, the production of enzymes such as protease, cellulase, or pectinase can be correlated with the extent of spore germination. germination is studied in vitro on agar-based media containing protein, cellulose, or pectin. the spores are immobilized on a permeable membrane mounted on the substrate-containing medium. at various times after inoculation the membrane-bound spores are remov ... | 1985 | 3913330 |
| morphogenetic activity of cytochalasins, cyanein, and monorden in botrytis cinerea. | | 1973 | 4771700 |
| influence of phosphate compounds on certain fungi and their preservative effects on fresh cherry fruit (prunus cerasus, l.). | studies were conducted to ascertain the retarding effects of four phosphate compounds (sodium hexametaphosphate, sodium tripolyphosphate, sodium tetraphosphate, and tetrasodium pyrophosphate) on molding of fresh cherries (prunus cerasus, l.). in vitro studies on their antimycotic effects against the most common fungal spoilers, penicillium expansum, rhizopus nigricans, and botrytis sp., were also carried out. sodium tetraphosphate appeared to be the most effective compound in preserving cherries ... | 1968 | 4295176 |
| volumetric aerobiological survey of conidial fungi in the north-east netherlands. ii. comparison of aerobiological data and skin tests with mould extracts in an asthmatic population. | a study was undertaken to see whether the principal airborne fungi in the north-east netherlands were also found to be the most reactive in skin testing. atmospheric samples were taken weekly with the andersen sampler, from april 1981, up to and including, june 1983. at the same time skin tests of 833 patients referred to the outpatient departments of pulmonology and allergology, because of recurrent bronchial obstructive complaints and a suspected allergy, were studied for strongly positive ski ... | 1985 | 4039540 |
| [effect of visible light and ultraviolet rays on the content of free amino acids and the amino acid composition of proteins of the fungus botrytis cinera pers]. | | 1971 | 5170636 |
| [mechanism of action of uv-rays on the metabolism of amino acids and organic acids in the fungus botrytis cinerea pers]. | | 1971 | 5104359 |
| the development of botrytis cinerea in irradiated strawberries during storage. | | 1971 | 5103389 |
| [effect of the fungus botrytis cinerea on incorporation of labeled amino acids by cabbage tissue ribosomes]. | | 1971 | 5167241 |
| [protein synthesis in the mitochondria of healthy and botrytis cinerea infected cabbage tissues]. | | 1973 | 4798315 |
| oxidation of alcohols by botrytis cinerea. | crude cell-free preparations of botrytis cinerea were found to oxidize straight-chain primary alcohols (except methanol), aromatic primary alcohols, and unsaturated primary alcohols. the resulting products were the corresponding aldehydes and an equal molar quantity of hydrogen peroxide. | 1971 | 5102778 |
| [a method of cultivating botrytis cinerea pers. for assaying various fungicides]. | | 1967 | 5629449 |
| mode of action of pollen in breaking resistance of vicia faba to botrytis cinerea. | | 1971 | 5106745 |
| the effect of pollen grains on infections caused by botrytis cinerea fr. | | 1968 | 5705482 |
| [antifungal and antimicrobial activity of beta-ionone and vitamin a derivatives]. | the antifungal and antimicrobic activity of some derivatives of beta-ionon and vitamin a was studied. these compounds (citral, pseudo-ionon. beta-ionon aldehyde c14, ketone c18 and its derivatives--4,18-diketone, alcohol c18, semicarbazide ketone c18), as well as vitamin a and its derivatives--retinal, acetate, retinoic acid--differ in composition, structure and substituents of c-atoms in beta-ionon ring and in polyenoic chain. fusarium solani, botrytis cenerea and verticillum dahliae ii, race 4 ... | 1983 | 6364128 |
| vitamin k5 as a fungistatic agent. | the effectiveness of vitamin k(5) in controlling the growth of different molds at varying ph levels in a culture medium, in tomato juice, and in several berry purees was studied. the molds studied were aspergillus, botrytis, hormodendrum, mucor, and penicillium. the results showed that vitamin k(5) was effective as a fungistatic agent at concentrations ranging from 0.006 to 0.02%. | 1965 | 5867645 |
| electron microscopy of botrytis cinerea conidia. | buckley, patricia m. (university of california, davis), virginia e. sjaholm, and n. f. sommer. electron microscopy of botrytis cinerea conidia. j. bacteriol. 91:2037-2044. 1966.-sections of germinating and nongerminating botrytis cinerea conidia were examined with an electron microscope. uranyl acetate or lead citrate provided contrast between membranes and cytoplasm. membrane-bounded, dense inclusions previously unreported in dormant spores were termed "storage bodies." whorled structures, sphe ... | 1966 | 5949251 |
| persistence of carbaryl residues in and on unprocessed and processed cauliflower curds. | carbaryl was applied three times at 15 days interval to cauliflower (brassica oleracea var. botrytis l.), and its persistence was determined spectrophotometrically at 0, 1, 3, 6, 10, and 15 days after the first and last treatments. in unprocessed cauliflower the prescribed tolerance level for carbaryl residues of 5 ppm was only reached on the sixth day, in processed cauliflower on the third day after application. pretreatment with washing + boiling/cooking on the other hand led to a considerable ... | 1982 | 6807280 |
| volatile and gaseous metabolites released by germinating seeds of lentil and maize cultivars with different susceptibilities to fusariosis and smut. | the effect of volatile and gaseous metabolites released by germinating seeds of lentil cultivars more and less susceptible to fusariosis on the germination of spores of mucor racemosus, trichoderma viride, verticillium dahliae and botrytis cinerea was found to depend rather on the fungal genus than on the lentil cultivar. however, spores of fusarium oxysporum reacted more sensitively during germination to the presence of exudates of both cultivars, when the more susceptible lentil displayed a st ... | 1980 | 7189738 |
| [on the variability and taxonomy of varieties and forms of the species botrytis mich. i. studies on culture-conditioned variation of morphological properties of the species botrytis]. | | 1966 | 5953535 |
| fungistatic effects of controlled atmospheres. | the fungistatic effects of controlled atmospheres composed of increased co(2) and decreased o(2) was studied in a manner such that the condition of stored fruit was not a factor in the growth of the fungi. varying concentrations of o(2) and co(2) were used. the fungi used were botrytis alli, rhizopus nigricans, and penicillium expansum. the results showed that controlled atmospheres, within the limits of concentrations usable for fruit storage, are effective fungistatic agents. | 1966 | 5951331 |
| [occurrence of an extracellular beta-(1 to 3)-glucanase from botrytis cinerea]. | like other fungi, botrytis cinera produces an exocellular beta-(1 leads to 3)-glucanase. this constitutive enzyme is produced only when botrytis is cultivated in a glucose depleted medium. the enzyme is precipitated by ammonium sulfate from 20 to 80% saturation. maximum activity is at ph between 5 and 6 and temperature between 40 and 50 degrees c; stability is good up to 30 degrees c. this enzyme cannot be applied to the treatment of wines containing botrytis glucan. | 1980 | 6771030 |
| [karyological studies on species and forms of the genus botrytis mich]. | | 1965 | 5894092 |
| [on the variability and taxonomy of varieties and forms of the species botrytis mich. ii. studies on the variability of the culture type with constant culture conditions]. | | 1966 | 5953536 |
| mycoparasitism of sclerotial fungi by teratosperma oligocladum. | sclerotia of sclerotinia minor were parasitized by teratosperma oligocladum, a recently described dematiaceous hyphomycete. the mycoparasite was cultured on living sclerotia placed on water agar and on sclerotia in moist sand. it grew poorly on several common laboratory media but growth in vitro was enhanced by supplements of soil extract and, especially, by aqueous extracts of sclerotia. sclerotia of s. minor, s. sclerotiorum, s. trifoliorum, sclerotium cepivorum, and botrytis cinerea were para ... | 1981 | 7198002 |
| antifungal compounds induced in the dual culture with phytolacca americana callus and botrytis fabae. | in order to investigate new metabolites which are only induced in a plant callus infected by a pathogenic fungus, dual cultures with combinations of 10 species of fungi and 6 plant cell lines from different species were established. among the combinations tested, the methanolic extract of a dual culture consisting of a plant cell line, phytolacca americana and a fungus, botrytis fabae showed a marked antifungal activity to cladosporium herbarum. the main active constituent of this extract was id ... | 1995 | 7546032 |
| vital fluorochromes as tracers for fungal growth studies. | eight fluorescent dyes were tested for staining the spores or mycelia of six fungi and for their translocation into new growth when the preloaded spores or mycelia were incubated on agar coated coverslips. the dyes studied were cellufluor, nile red, fluorescein diacetate (fda), carboxyfluorescein diacetate (cfda), chloromethylfluorescein diacetate (cmfda), aminochloromethyl coumarin (cmac), and the carbocyamines diic18(3) and dioc18(3). the fungi on which the dyes were tested included botrytis c ... | 1995 | 7578589 |
| antimicrobial activities of indolocarbazole and bis-indole protein kinase c inhibitors. ii. substitution on maleimide nitrogen with functional groups bearing a labile hydrogen. | new compounds, structurally related to the potent protein kinase c inhibitor staurosporine, and substituted on the imide nitrogen with a functional group bearing a labile hydrogen (hydroxymethyl, amino, hydroxy), were synthesized. their in vitro inhibitory potencies towards protein kinase c and protein kinase a showed that n-hydroxymethyl and n-hydroxy substitution, unlike alkyl substitution, can provide efficient protein kinase c inhibitors. the antimicrobial activities of these new compounds a ... | 1995 | 7592032 |
| a volumetric-aerobiologic study of seasonal fungus prevalence inside and outside dwellings of asthmatic patients living in northeast netherlands. | air sampling with an andersen sampler was carried out over a period of 21 mo, in 28 houses of asthmatic patients with an allergy for moulds. inside, the livingroom, bedroom, and kitchen were studied, and an additional outside sample was always taken. indoors, penicillium showed the highest concentration, especially in the autumn and winter months, while aspergillus and yeasts were also found frequently. the outside air was dominated by cladosporium and alternaria during summer months and botryti ... | 1984 | 6507952 |
| air-borne fungi in the air of barcelona (spain). iv. various isolated genera. | during a two-year survey on the air-borne fungi in the atmosphere of barcelona (spain), the following genera were isolated in decreasing order: aureobasidium, rhizopus, mucor, arthrinium, phoma, fusarium, trichoderma, and botrytis. | 1980 | 7190223 |
| polygalacturonase produced in apple tissue decayed by botrytis cinerea. | an exo-polygalacturonase with an isoelectric point of 4.6 and an apparent molecular weight of 45 kda was isolated from apple tissue decayed by botrytis cinerea. this isozyme had a similar isoelectric point, optimum ph, and mode of action as an isozyme produced in liquid culture by b. cinerea. the enzyme produced in the decayed tissue was less sensitive to lower ph and less inhibited by cacl2, mgcl2, or nacl than the enzyme produced in culture. such changes in the properties of the enzyme produce ... | 1995 | 7627131 |
| biological activity of cyclopaldic acid, a major toxin of seiridium cupressi, its six derivatives, and iso-cyclopaldic acid. | cyclopaldic acid (ca), its six derivatives, and iso-cyclopaldic acid (isoca) were assayed for toxicity to cuttings of three species of cypress, as well as to mung bean, oat, and tomato explants. toxicity to host and non-host plants of ca derivatives having one or both of the aldehyde groups transformed was less than that of ca. shoot tissues of cupressus macrocarpa artificially infected by seiridium cupressi leached electrolytes more than those of c. sempervirens and c. arizonica. ca, isoca, and ... | 1995 | 7648024 |
| chemoprevention of azoxymethane-induced colon carcinogenesis by dietary feeding of s-methyl methane thiosulfonate in male f344 rats. | modifying effects of dietary exposure of s-methyl methane thiosulfonate (mmts) isolated from cauliflower brassica oleracea l. var. botrytis on rat colon carcinogenesis induced by azoxymethane (aom) and on the expression of cell proliferation biomarkers were investigated in two experiments. in experiment 1, male f344 rats were given three s.c. injections of aom (15 mg/kg body weight) and fed 100 ppm mmts for 5 weeks, starting 1 week before the first dose of aom. the frequency of colonic aberrant ... | 1995 | 7664279 |
| [molds in foods of the czechoslovak socialistic republik (author's transl)]. | the mycoflora of 13.527 g of food powders which were normal upon sensory evaluation as well of 22.017 pieces of surface sterilized grains and nuts was examined in the czechoslovak socialist republic. 147.027 colonies of 44 genera of molds were isolated and identified from samples. colonies of penicllium sp., aspergillus sp., and cladosporium sp. occured most often, less often or quite infrequently rhizopus sp., mucor sp., absidia sp., alternaria sp., circinella sp., trichoderma sp., botrytis sp. ... | 1981 | 7269803 |
| antibiotic production and biocontrol activity by bacillus subtilis cl27 and bacillus pumilus cl45. | bacillus subtilis cl27 and b. pumilus cl45 showed similar activity against botrytis cinerea in in vitro plate assays. in a seedling bioassay, however, b. subtilis cl27 had activity similar to a commercial fungicide while b. pumilus cl45 failed completely to prevent seedling damping-off caused by bot. cinerea. antibiotic production by the two bacillus strains was found to depend on the growth substrate and highest antibiotic production was found on media based on homogenized cabbage tissue. antib ... | 1995 | 7698955 |
| partial characterization of chitinolytic enzymes from streptomyces albidoflavus. | streptomyces albidoflavus nrrl b-16746 secreted three types of chitinolytic enzymes: n-acetyl-glucosaminidase, chitobiosidase and endochitinase. optimal activity for all three types of enzymes occurred at ph 4-6; however 55-74% of the chitobiosidase and endochitinase activity was detectable at ph 8-10. chitobiosidase activity originated from two strongly acidic (pi < 3.0) proteins with molecular mass of 27 kda and 34 kda, while endochitinase activity originated from five major acidic proteins (p ... | 1995 | 7766226 |
| parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of trichoderma harzianum against phytopathogenic fungi. | chitinase, beta-1,3-glucanase, and protease activities were formed when trichoderma harzianum mycelia, grown on glucose as the sole carbon source, were transferred to fresh medium containing cell walls of botrytis cinerea. chitobiohydrolase, endochitinase, and beta-1,3-glucanase activities were immunologically detected in culture supernatants by western blotting (immunoblotting), and the first two were quantified by enzyme-linked immunosorbent assay. under the same conditions, exogenously added ... | 1994 | 7811076 |
| hormone effects on cosmarium botrytis cell division. | the synchronous generation time for cosmarium botrytis menegh. was 48 h at ph 8.0, and 72 h at ph 5.3. cells divided 2 to 3 prior to the end of the dark phase in a 15 h light/9 h dark cycle at 21 degrees c. kinetin at concentrations of 0.01 to 1.0 mg/ml, added to cells at the beginning of the light cycle, eliminated the initial lag phase at both ph 5.3 and ph 8.0, and all cells divided until 24 h. indole-acetic acid in concentrations of 0.03 to 0.06 mg/ml decreased the lag phase to less than 48 ... | 1981 | 7273854 |