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age and sex dependence of the effects of an aqueous extract of physalis alkekengi fruits on rat hepatic glucose 6-p dehydrogenase activity.intraperitoneal injections of an aqueous extract of winter cherry fruits (physalis alkekengi) to new-born, weanling and adult female rats and to weanling and adult male rats had no effect on body weight, liver weight and liver cytosol protein content. the specific activities of hepatic glucose 6-p dehydrogenase (an estrogen induced protein) in rats of different age and sex groups in terms of mu/mg protein were: treated new-born females, 15.9 +/- 0.5; control, 29.1 +/- 0.6; treated weanling femal ...19958574927
comparison of the effects of an aqueous extract of physalis alkekengi fruits and/or various doses of 17-beta-estradiol on rat estrous cycle and uterine glucose 6-phosphate dehydrogenase activity.intraperitoneal injections of an aqueous extract of winter cherry fruits (physalis alkekengi) to adult normal cycling female rats produced 100% diestrus and diminished uterine glucose 6-p dehydrogenase activity (an estrogen-induced protein) by 52%. daily doses of 1.88, 3.75 and 7.5 micrograms 17-beta-estradiol administered intraperitoneally to adult female rats for a period of 6-8 days prolonged proestrus or estrus and increased uterine glucose 6-p dehydrogenase activity by 11.5%, 26.9% and 82.1 ...19958788592
protein chemotaxonomy of the solanaceae. vi. amino acid sequence of ferredoxin from nicotian tabacum.the complete amino acid sequence of [2fe-2s] ferredoxin from nicotiana tabacum has been determined by automated edman degradation of the entire cm-protein and of the peptides obtained by trypsin and asp-n endoproteinase digestions. this ferredoxin exhibited 9, 10, 8, and 10 differences respectively in its amino acid sequence, when compared with the ferredoxins of datura stramonium, d. metel, d. arborea, and physalis alkekengi var.francheti but 17-28 differences for other angiosperms, and 34-37 d ...19999989672
[karyotype analysis of physalis pubescens chromosome].to study the karyotype of physalis pubescens l..200415307679
nocturnal uptake and assimilation of nitrogen dioxide by c3 and cam plants.in order to investigate nocturnal uptake and assimilation of no2 by c3 and crassulacean acid metabolism (cam) plants, they were fumigated with 4 microl l(-1) 15n-labeled nitrogen dioxide (no2) for 8 h. the amount of no2 and assimilation of no2 by plants were determined by mass spectrometry and kjeldahl-nitrogen based mass spectrometry, respectively. c3 plants such as kenaf (hibiscus cannabinus), tobacco (nicotiana tabacum) and ground cherry (physalis alkekengi) showed a high uptake and assimilat ...200515948596
[antioxidant activity of the fruits and hydrophilic compounds of physalis alkekengi].physalis alkekengi l. (bladder cherry, chinese lantern, winter cherry) is an unusual species of the family solanaceae. although accumulation of alkaloids is characteristic to solanaceae species, and accordingly the root and above ground parts of p. alkekengi are toxic, its fruits are in exceptionally edible. the present paper deals with the investigation of antioxidant hydrophilic compounds of the fruits in order to find correlation between the quantity of the constituents and antioxidant capaci ...200920183952
an ultra-pressure liquid chromatography-tandem mass spectrometry method for the simultaneous determination of three physalins in rat plasma and its application to pharmacokinetic study of physalis alkekengi var. franchetii (chinese lantern) in rats.an ultra-high pressure liquid chromatography-tandem mass spectrometry (uplc-ms/ms) method was developed for the quantification of three major ingredients in chinese lantern preparations (clp) in rat plasma. following extraction by ethyl acetate, the analytes were separated on an acquity uplc beh shield rp c(18) column using a gradient mobile phase system of acetonitrile-water. electrospray ionization (esi) tandem interface was employed prior to mass spectrometric detection. the calibration curve ...201221996065
modulation of the pituitary and basomedial hypothalamic lysyl-aminopeptidase activities be beta-estradiol and/or an aqueous extract of physalis alkekengi fruits.injections of an aqueous extract of winter cherry fruits (physalis alkekengi) to adult female cycling rats by an intraperitoneal route resulted in the diminution of the pituitary lysyl-aminopeptidase (lys-ap) activity by 50% and that of the basomedial hypothalamus (bmh) by 45%. administration of daily doses of 3.75, 7.5, and 15 micrograms beta-estradiol for a period of 5-8 days to such animals increased pituitary lys-ap activity from 31% to 61.5% and that of bmh from 20% to 87%, respectively. ad ...19968998678
(1)h nmr spectroscopy-guided isolation of new sucrose esters from physalis alkekengi var. franchetii and their antibacterial activity.ten new sucrose esters, physakengoses a-j (1-10), were isolated from the aerial parts of physalis alkekengi var. franchetii under the guidance of (1)h nmr spectroscopy. their structures were determined by spectroscopic analyses (hresims, 1d and 2d nmr, and esims) and chemical methods. these new compounds were tested for antibacterial activities against staphylococcus aureus, bacillus subtilis, pseudomonas aeruginosa and escherichia coli. among them, compounds 2 and 5-8 showed potent inhibitory e ...201627642039
calystegins of physalis alkekengi var. francheti (solanaceae). structure determination and their glycosidase inhibitory activities.five calystegins were extracted from the roots of physalis alkekengi var. francheti (solanaceae) with hot water and purified to homogeneity by the combination of a variety of ion-exchange column chromatographies. their structures have been determined from the 1h- and 13c-nmr spectral data, and two of the compounds were identified as calystegins a3 and b2, which have been isolated from the roots of calystegia sepium (convolvulaceae). two of the remaining three were found to be 1 alpha, 3 alpha, 4 ...19957744059
effects of an aqueous extract of physalis alkekengi fruit on estrus cycle, reproduction and uterine creatine kinase bb-isozyme in rats.intraperitoneal injections of the aqueous extract of winter cherry fruits (physalis alkekengi) to female rats produced 100% diestrus. the rats resumed their normal estrus cycle upon withdrawal of this extract. although there was no significant decrease in the number of implantation sites, the number of pups born to rats decreased by 96% with extract administration. treatment with this extract had no effect on body weight, uterus weight, plasma protein level or plasma total creatine kinase activi ...19911753789
improvement of intestinal microflora balance by polysaccharide from physalis alkekengi var. francheti.a water‑soluble polysaccharide fraction (ppsb) was fractionated from physalis alkekengi var. francheti and purified using deae‑52 cellulose and sephadex g‑200 chromatography. the physicochemical properties of ppsb and its molecular activities involved in the improvement of intestinal microflora balance were evaluated. ppsb (12.5‑25.0 mg/ml) was shown to promote the growth of lactobacillus delbrueckii and to inhibit the growth of escherichia coli. denaturing gradient gel electrophoresis analysis ...201424297313
heterotopic expression of mpf2 is the key to the evolution of the chinese lantern of physalis, a morphological novelty in solanaceae.morphological novelties arise through changes in development, but the underlying causes of such changes are largely unknown. in the genus physalis, sepals resume growth after pollination to encapsulate the mature fruit, forming the "chinese lantern," a trait also termed inflated-calyx syndrome (ics). stmads16, which encodes a mads-box transcription factor, is expressed only in vegetative tissues in solanum tuberosum. its ortholog in physalis pubescens, mpf2, is expressed in floral tissues. knock ...200515824316
six new physalins from physalis alkekengi var. franchetii and their cytotoxicity and antibacterial activity.six new physalin steroids, 7β-methoxylisophysalin b (1), 7β-methoxylphysalin c (2), physalin v (3), physalin vi (4), physalin vii (5), isophysalin i (6), together with 20 known physalins (7-26) were isolated from calyces of physalis alkekengi var. franchetii. structures of the new compounds were revealed through 1d and 2d nmr and mass spectroscopic methods. compounds 1-26 were evaluated for cytotoxicity against human hl-60, smmc-7721, a-549, mcf-7 and sw-480, and the results indicated that compo ...201627215128
first report of fingerprinting dried herbal products using a subtractive diversity array.introduction sequence - independent microarrays have never been used to identify and authenticate dried herbal plants.201323828332
adjuvant effect of polysaccharide from fruits of physalis alkekengi l. in dna vaccine against systemic candidiasis.adjuvant effect mediated by polysaccharide (ppsb) isolated from the fruits of physalis alkekengi l. in dna vaccine was evaluated in mice. recombinant plasmid containing epitope c (lkvirk) from heat shock protein 90 (hsp90) of candida albicans (c. albican) was used as dna vaccine (pd-hsp90c). the results indicated that ppsb significantly enhanced specific antibody titers igg, igg1, igg2b, and concentration of il-2 and il-4 in sera of mice immunized with pd-hsp90c (p<0.05). more importantly, it wa ...201424815404
in vitro effects on intestinal bacterium of physalins from physalis alkekengi var. francheti.intestinal probiotic bacterium stimulative activity-guided fractionation of physalis alkekengi var. francheti calyces extract resulted in the isolation of four new physalins (1-4). their structures were elucidated as 5α, 6β-dihydroxy-25, 27-dihydro-7-deoxyphysalin a (1), 5α, 6β-dihydroxyphysalin r (2), 3β-hydroxy-2-hydrophysalin a (3) and 5α-hydroxy-7-dehydro-25, 27-dihydro-7-deoxyneophysalin a (4) by uv, ms, 1d and 2d nmr spectroscopy. growth curves of lactobacillus delbrueckii and escherichia ...201222940088
identification and quantification of zeaxanthin esters in plants using liquid chromatography-mass spectrometry.it has been suggested that lutein and zeaxanthin may decrease the risk for age-related macular degeneration. surprisingly, oleoresins rich in zeaxanthin are not yet available on the market. several authors have reported enhanced stability of esterified xanthophylls, so plants containing zeaxanthin esters were investigated to establish valuable sources for the production of durable oleoresins. liquid chromatography-atmospheric pressure chemical ionization mass spectrometry [lc-(apci)ms] was used ...200314611169
inhibitory effects of physalis alkekengi l., alcea rosea l., bunium persicum b. fedtsch. and marrubium vulgare l. on mushroom tyrosinase.the key enzyme in the process of melanin biosynthesis is tyrosinase. skin hyperpigmentation and browning of foods are undesirable phenomena which tyrosinase represents. therefore, tyrosinase inhibitors have been used increasingly for medicinal and cosmetic products.201525866725
amino acid sequence of ferredoxin from physalis alkekengi var. francheti.the complete amino acid sequence of [2fe-2s] ferredoxin from physalis alkekengi var. francheti has been determined by automated edman degradation of the entire cm-protein and of the peptides obtained by trypsin and endoproteinase asp-n digestions. this ferredoxin exhibited ten, ten, and nine differences respectively in the amino acid sequence, when compared with the ferredoxins of datura stramonium, d. metel, and d. arborea, but 21-28 differences for other angiosperms, and 34-37 differences for ...19989862138
thielavialides a-e, nor-spiro-azaphilones, and a bis-spiro-azaphilone from thielavia sp. pa0001, an endophytic fungus isolated from aeroponically grown physalis alkekengi.four new nor-spiro-azaphilones, thielavialides a-d (1- 4), a new bis-spiro-azaphilone, thielavialide e (5), together with pestafolide a (6), were isolated from the endophytic fungal strain, thielavia sp. pa0001, occurring in the healthy leaf tissue of aeroponically grown physalis alkekengi. the structures and relative configurations of 1-5 were established on the basis of their ms and nmr data. possible biosynthetic pathways to thielavialides a-e (1- 5) from pestafolide a (6), some involving a f ...201424882589
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