| [production of pectic enzymes by pseudomonas viridiflava]. | | 1975 | 1234619 |
| cloning and characterization of a pectate lyase gene from the soft-rotting bacterium pseudomonas viridiflava. | pseudomonas viridiflava is a soft-rotting pathogen of harvested vegetables that produces an extracellular pectate lyase (pl) responsible for maceration of plant tissue. a pel gene encoding pl was cloned from the genome of strain sj074 and efficiently expressed in escherichia coli. after a series of deletion subclonings and analysis by transposon mutagenesis, the pel gene was located in a 1.2-kb psti-bglii genomic fragment. this fragment appears to contain a promoter at the psti end required for ... | 1992 | 1325218 |
| cloning of pectate lyase gene pel from pseudomonas fluorescens and detection of sequences homologous to pel in pseudomonas viridiflava and pseudomonas putida. | pectate lyase (pl) depolymerizes pectin and other polygalacturonates (pgas) and is thought to play a role in bacterial invasion of plants. production of pl by the soft-rotting pathogen pseudomonas fluorescens cy091 is regulated by ca2+. in the presence of ca2+, this bacterium constitutively synthesizes pl in media containing glucose, glycerol, or pga and excretes over 87% of total pl into culture fluids. in the absence of ca2+, the organism fails to use pga as a carbon source and produces very l ... | 1991 | 1906062 |
| fosfadecin and fosfocytocin, new nucleotide antibiotics produced by bacteria. | two new nucleotide antibiotics, fosfadecin and fosfocytocin, have been isolated from the culture filtrates of pseudomonas viridiflava pk-5 and pseudomonas fluorescens pk-52, respectively. these antibiotics were purified by column chromatographies using adsorption, gel filtration and ion exchange resins. on the basis of the spectroscopic and degradation studies, the chemical structures of fosfadecin and fosfocytocin were determined. these antibiotics were either enzymatically or chemically hydrol ... | 1990 | 2182591 |
| antimicrobial activity of naphthoquinones from fusaria. | twenty-two naphthoquinone compounds isolated or derived synthetically from culture extracts of fusarium solani and f. oxysporum were examined for antimicrobial activity. fifteen exhibited antibiotic activity against staphylococcus aureus, and 12 were active against streptococcus pyogenes, but none were active at the highest rate of 128 micrograms/ml against escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, serratia marcescens, or pseudomonas aeruginosa. of 8 plant path ... | 1990 | 2233983 |
| analysis of pectate lyases produced by soft rot bacteria associated with spoilage of vegetables. | isoelectric focusing (ief) profiles of pectate lyases (pls) produced by five different groups of soft rot bacteria were analyzed by using the combined techniques of thin-layer polyacrylamide gel ief and agarose-pectate overlay activity staining. four strains of soft rot erwinia spp. produced three or more pl isozymes. all of eight pseudomonas viridiflava strains examined produced one single pl with a pi of 9.7. all 10 of pseudomonas fluorescens strains produced two pls; the major one had a pi of ... | 1989 | 2764574 |
| [obtainment and ultrastructural study of l forms induced by glycocoll in pseudomonas viridiflava]. | | 1974 | 4410250 |
| pseudomonas viridiflava (burkholder, 1930; clara 1934). | | 1970 | 4923557 |
| a global regulator of secondary metabolite production in pseudomonas fluorescens pf-5. | mutations in the apda (for antibiotic production) gene of the plant root-colonizing bacterium pseudomonas fluorescens pf-5 pleiotropically abolish the production of an array of antibiotics, including pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol, as well as the production of tryptophan side chain oxidase, hydrogen cyanide, and an extracellular protease. the lack of production of secondary metabolites by apda- mutants was correlated with the loss of inhibition of the phytopathogenic f ... | 1995 | 7592389 |
| molecular characterization of two gene loci required for production of the key pathogenicity factor pectate lyase in pseudomonas viridiflava. | four pleiotropic mutants of pseudomonas viridiflava strain pj-08-6a that were deficient in production of both pectate lyase (pel) and protease (prt) were isolated following transposon mutagenesis. unlike secretion-defective (out-) mutants, these four showed no accumulation of enzymes within the cells. southern hybridization analysis revealed that each mutant had tn5 inserted in one of two ecori genomic fragments. these ecori fragments (5.2- and 6.3-kb) appeared to contain two distinct gene loci, ... | 1994 | 8012049 |
| the repb gene required for production of extracellular enzymes and fluorescent siderophores in pseudomonas viridiflava is an analog of the gaca gene of pseudomonas syringae. | two genes, designated repa and repb, are involved in the regulation of the synthesis of extracellular pectate lyase, protease, and alginate in pseudomonas viridiflava. the repa gene has been shown to encode a protein highly homologous to several bacterial sensors in the two-component regulator family including the lema of pseudomonas syringae. in this study, the repb locus, initially identified in a 6.3-kb ecori genomic fragment of p. viridiflava, was further characterized. results obtained from ... | 1996 | 8742358 |
| distribution of pseudomonas syringae pathovars into twenty-three o serogroups. | serological reactions of pseudomonas syringae and pseudomonas viridiflava were studied by ouchterlony double diffusion. a total of 55 polyclonal antisera, containing anti-lipopolysaccharide (anti-lps) precipitating antibodies, were cross-tested against antigenic suspensions of 51 strains. twenty-three o serogroups were defined, primarily on the reaction of the type strains. two families of o serogroups showed antigenic crossreactivities (pha, mop1, mop2, mop3, hel1, hel2, and syr1; persavtom1, p ... | 1996 | 8779574 |
| assessment of genetic diversity among strains of pseudomonas syringae by pcr-restriction fragment length polymorphism analysis of rrna operons with special emphasis on p. syringae pv. tomato. | phylogenetic relationships among 77 bacterial strains belonging to pseudomonas syringae and pseudomonas viridiflava species were assessed by analysis of the pcr-restriction fragment length polymorphism (rflp) patterns of three dna fragments corresponding to rrs and rrl genes and the internal transcribed spacer, its1. no difference among all strains in rrs and rrl genes was observed with 14 restriction enzymes, which confirms the close relationships existing between these two species. the nucleot ... | 1997 | 9023928 |
| molecular cloning, characterization, and mutagenesis of a pel gene from pseudomonas syringae pv. lachyrmans encoding a member of the erwinia chrysanthemi pelade family of pectate lyases. | the pels gene from pseudomonas syringae pv. lachrymans 859 was cloned by heterologous expression in nonpectolytic p. syringae pv. syringae buvs1, using genomic dna libraries constructed with two novel broad-host-range cosmid vectors, pcpp34 and pcpp47. screening of p. syringae pv. syringae transconjugants for the ability to pit pectate media at ph 6.0 and 8.5 yielded several overlapping clones of the same dna region. ultrathin-layer isoelectric focusing gels, activity-stained with diagnostically ... | 1997 | 9100381 |
| identification of gene loci controlling pectate lyase production and soft-rot pathogenicity in pseudomonas marginalis. | pseudomonas marginalis is an important postharvest pathogen capable of causing soft rot in a wide variety of harvested fruits and vegetables. following transposon mutagenesis, we isolated two groups of p. marginalis cy091 mutants deficient in production of pectate lyase (pel) and soft-rot pathogenicity in plants. the first group, designated pel-, was caused by the insertion of tn5 into a pel structural gene, and the second group, designated lema-, was caused by the insertion of tn5 into a regula ... | 1997 | 9165701 |
| ecomycins, unique antimycotics from pseudomonas viridiflava. | a novel family of peptide antimycotics, termed ecomycins, is described from pseudomonas viridiflava, a plant-associated bacterium. ecomycins b and c have molecular masses of 1153 and 1181. they contain equimolar amounts of a beta hydroxyaspartic acid, homoserine, threonine, serine, alanine, glycine and one unknown amino acid. fatty acids were detectable after hydrolysis, methylation and gas chromatography and mass spectroscopy. the ecomycins have significant bioactivities against a wide range of ... | 1998 | 9717277 |
| the pseudomonas syringae pv. tomato hrpw protein has domains similar to harpins and pectate lyases and can elicit the plant hypersensitive response and bind to pectate. | the host-specific plant pathogen pseudomonas syringae elicits the hypersensitive response (hr) in nonhost plants and secretes the hrpz harpin in culture via the hrp (type iii) secretion system. previous genetic evidence suggested the existence of another harpin gene in the p. syringae genome. hrpw was found in a region adjacent to the hrp cluster in p. syringae pv. tomato dc3000. hrpw encodes a 42. 9-kda protein with domains resembling harpins and pectate lyases (pels), respectively. hrpw has ke ... | 1998 | 9748456 |
| identification of a novel ice-nucleating bacterium of antarctic origin and its ice nucleation properties. | a novel ice-nucleating bacterium (inb) was isolated from ross island, antarctica. inbs could be isolated more frequently than was generally thought. inb strain in-74 was found in the white colony group. strain in-74 was identified from its taxonomic characteristics as a novel inb, pseudomonas antarctica in-74. when strain in-74 was cultured aerobically in a medium consisting of the ice-nucleating broth (ph 7.0) for 6 days at 4 degrees c, the ice-nucleating activity of strain in-74 cells was obta ... | 1999 | 10191036 |
| dna relatedness among the pathovars of pseudomonas syringae and description of pseudomonas tremae sp. nov. and pseudomonas cannabina sp. nov. (ex sutic and dowson 1959). | a total of 48 pathovars of pseudomonas syringae and eight related species were studied by dna-dna hybridization (s1 nuclease method) and ribotyping. the existence of nine discrete genomospecies was indicated. genomospecies 1 corresponded to p. syringae sensu stricto and included p. syringae pathovars syringae, aptata, lapsa, papulans, pisi, atrofaciens, aceris, panici, dysoxyli and japonica. genomospecies 2 included p. syringae pathovars phaseolicola, ulmi, mori, lachrymans, sesami, tabaci, mors ... | 1999 | 10319466 |
| production and comparison of peptide siderophores from strains of distantly related pathovars of pseudomonas syringae and pseudomonas viridiflava lmg 2352. | the production of peptide siderophores and the variation in siderophore production among strains of pseudomonas syringae and pseudomonas viridiflava were investigated. an antibiose test was used to select a free amino acid-containing agar medium favorable for production of fluorescent siderophores by two p. syringae strains. a culture technique in which both liquid and solid asparagine-containing culture media were used proved to be reproducible and highly effective for inducing production of si ... | 2000 | 10618243 |
| natural incidence of endophytic bacteria in pea cultivars under field conditions. | pea plants grown in the field were used to study the natural incidence of endophytic bacteria in the stem. eleven pea cultivars at the flowering stage were screened for the presence of endophytic bacteria using a printing technique with surface disinfested stem cross-sections on 5% trypticase soy agar (tsa). five cultivars showed colonization. cultivar twiggy showed the highest and most consistent colonization and was further investigated. stems of cv. twiggy at the pod stage were analyzed for e ... | 2000 | 11109492 |
| the structure of the pyoverdin isolated from various pseudomonas syringae pathovars. | from seven different pathovars of pseudomonas syringae representing various genetic subgroups, and one strain of pseudomonas viridiflava the same pyoverdin siderophore (1) was isolated, probably identical with the pyoverdin whose amino acid composition (but not their sequence) had been reported before. 1 is the first pyoverdin where two of the ligands for fe3+ are beta-hydroxy asp units. its remarkably high complexing constant for fe3+ at ph 5 as compared with other pyoverdins offers a definite ... | 2001 | 11724369 |
| pseudomonas viridiflava and p. syringae--natural pathogens of arabidopsis thaliana. | we report the isolation and identification of two natural pathogens of arabidopsis thaliana, pseudomonas viridiflava and pseudomonas syringae, in the midwestern united states. p. viridiflava was found in six of seven surveyed arabidopsis thaliana populations. we confirmed the presence in the isolates of the critical pathogenicity genes hrps and hrpl. the pathogenicity of these isolates was verified by estimating in planta bacterial growth rates and by testing for disease symptoms and hypersensit ... | 2002 | 12481991 |
| high-performance liquid chromatography analyses of pyoverdin siderophores differentiate among phytopathogenic fluorescent pseudomonas species. | the relationship of pyoverdins produced by 41 pathovars of pseudomonas syringae and by phytopathogenic pseudomonas species was investigated. a high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typical fe(iii)-chelated pyoverdins. nonfluorescent strains were found in pseudomonas amygdali, pseudomonas ... | 2003 | 12571041 |
| identification of an emergent and atypical pseudomonas viridiflava lineage causing bacteriosis in plants of agronomic importance in a spanish region. | pseudomonas strains with an atypical lopat profile (where lopat is a series of determinative tests: l, levan production; o, oxidase production; p, pectinolitic activity; a, arginine dihydrolase production; and t, tobacco hypersensibility) can be regarded as emergent pathogens in the principality of asturias (spain), where they have been causing, since 1999, severe damage in at least three taxonomically unrelated orchard plants of agronomic importance: common bean (phaseolus vulgaris), kiwifruit ... | 2003 | 12732569 |
| genetic diversity, recombination and cryptic clades in pseudomonas viridiflava infecting natural populations of arabidopsis thaliana. | species-level genetic diversity and recombination in bacterial pathogens of wild plant populations have been nearly unexplored. pseudomonas viridiflava is a common natural bacterial pathogen of arabidopsis thaliana, for which pathogen defense genes and mechanisms are becoming increasing well known. the genetic variation contained within a worldwide sample of p. viridiflava collected from wild populations of a. thaliana was investigated using five genomic sequence fragments totaling 2.3 kb. two d ... | 2005 | 15489535 |
| characterization of pseudomonas pathovars isolated from rosaceous fruit trees in east algeria. | a survey of bacterial diseases due to pseudomonas on rosaceous fruit trees was conducted. in forty two orchards located in the constantine region ( east algeria). pseudomonas isolates were identified on the bases of their cultural and biochemical characteristics . a total of fifty nine phytopathogenic bacteria were isolated from diseased pome and stone fruit trees. thirty one strains comparable to pseudomonas syringae pv. syringae were isolated from cherry (prunus avium l.), plum (p. domestica l ... | 2004 | 15756824 |
| identification of virulence-associated genes of pseudomonas viridiflava activated during infection by use of a novel ivet promoter probing plasmid. | analysis of virulence mechanisms of plant pathogens is often limited by the lack of genetic tools that can be used to identify genes that are preferentially expressed during their interactions with plants. in the present study, we used the newly constructed ivet (in vivo expression technique) plasmid pivigk and the corresponding antibiotic resistance-based selection method to identify genes that encode pathogenicity factors of the soft rot-causing bacterium pseudomonas viridiflava. these include ... | 2006 | 16550466 |
| presence/absence polymorphism for alternative pathogenicity islands in pseudomonas viridiflava, a pathogen of arabidopsis. | the contribution of arms race dynamics to plant-pathogen coevolution has been called into question by the presence of balanced polymorphisms in resistance genes of arabidopsis thaliana, but less is known about the pathogen side of the interaction. here we investigate structural polymorphism in pathogenicity islands (pais) in pseudomonas viridiflava, a prevalent bacterial pathogen of a. thaliana. pais encode the type iii secretion system along with its effectors and are essential for pathogen rec ... | 2006 | 16581904 |
| the effects of streptomycin, desiccation, and uv radiation on ice nucleation by pseudomonas viridiflava. | streptomycin (100 micrograms per milliliter), desiccation (over caso(4)), and ultraviolet radiation (4500 microwatts per square centimeter at 254 nanometers for 15 minutes) reduced ice nucleation activity by pseudomonas viridiflava strain w-1 as determined by freezing drops of the bacterial suspensions. highest residual ice nucleation activity by dead cells was obtained by desiccation, although no freezing above -3.5 degrees c was detected. the rate and extent of loss of ice nucleation activity ... | 1986 | 16664748 |
| variation in resistance and virulence in the interaction between arabidopsis thaliana and a bacterial pathogen. | we examined patterns of variation and the extent of local adaptation in the interaction between the highly selfing annual weed arabidopsis thaliana and its foliar bacterial pathogen pseudomonas viridiflava by cross-infecting 23 bacterial isolates with 35 plant lines collected from six fallow or cultivated fields in the midwest, usa. we used two measures of resistance and virulence: bacterial count in the leaf and symptom development four days after infection. we found variation in resistance in ... | 2006 | 17017057 |
| fitness consequences of infection of arabidopsis thaliana with its natural bacterial pathogen pseudomonas viridiflava. | variation in plant resistance to pathogen infection is commonly observed in interactions between wild plants and their foliar pathogens. models of host-pathogen interactions indicate that a large cost of infection is generally necessary to maintain this variation, yet there is limited evidence that foliar pathogens cause detectable fitness reductions in wild host plants. most published work has focused on fungal pathogens. pseudomonas viridiflava, a common bacterial pathogen of the annual weed a ... | 2007 | 17180370 |
| the role of pectate lyase and the jasmonic acid defense response in pseudomonas viridiflava virulence. | pseudomonas viridiflava is a common pathogen of arabidopsis thaliana in wild populations, yet very little is known about mechanisms of resistance and virulence in this interaction. we examined the induced defense response of a. thaliana to several strains of p. viridiflava collected from this host by quantifying the expression of pr-1 and lox2/pdf1.2, which serve as markers for induction of the salicylic and jasmonic acid (ja) pathways, respectively. growth of these strains then was assessed on ... | 2007 | 17313166 |
| molecular evolution of pathogenicity-island genes in pseudomonas viridiflava. | the bacterial pathogen pseudomonas viridiflava possesses two pathogenicity islands (pais) that share many gene homologs, but are structurally and phenotypically differentiated (t-pai and s-pai). these pais are paralogous, but only one is present in each isolate. while this dual presence/absence polymorphism has been shown to be maintained by balancing selection, little is known about the molecular evolution of individual genes on the pais. here we investigate genetic variation of 12 pai gene loc ... | 2007 | 17720907 |
| apoplastic polyamine oxidation plays different roles in local responses of tobacco to infection by the necrotrophic fungus sclerotinia sclerotiorum and the biotrophic bacterium pseudomonas viridiflava. | the role of polyamine (pa) metabolism in tobacco (nicotiana tabacum) defense against pathogens with contrasting pathogenic strategies was evaluated. infection by the necrotrophic fungus sclerotinia sclerotiorum resulted in increased arginine decarboxylase expression and activity in host tissues, as well as putrescine and spermine accumulation in leaf apoplast. enhancement of leaf pa levels, either by using transgenic plants or infiltration with exogenous pas, led to increased necrosis due to inf ... | 2008 | 18583531 |
| in vitro and in vivo inhibition of plant polyamine oxidase activity by polyamine analogues. | polyamine oxidase from avena sativa l. cv. cristal seedlings was purified to homogeneity using a simple four-step purification protocol including an infiltration washing technique. the enzyme had a high affinity for spermidine and spermine (k(m) approximately 5.5 and 1.2 microm, respectively), and also oxidized norspermidine (k(m) approximately 64.0 microm). natural and synthetic diamines, cyclohexylamine, the putrescine analogue 1-aminooxy-3-aminopropane, and several polyamine analogues had inh ... | 2008 | 18783804 |
| the hrp genes of pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce. | pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce. through transposon insertion into p. cichorii strain spc9018 we produced two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce. analyses showed that a transposon was inserted into the hrpg gene in 4-57 and the hrct gene in 2-99. nucleotide sequences of the hrp genes of spc9018 are homologous to those of pseudomonas viridiflava bs group strains. the pathogenicity of 4-57 on eggplant was restored by ... | 2008 | 18832299 |
| occurrence of indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet. | abstract a relatively high percentage of epiphytic bacteria on pear leaf and fruit surfaces had the ability to produce indole-3-acetic acid (iaa) in culture media supplemented with tryptophan. while over 50% of the strains produced at least small amounts of iaa in culture, about 25% of the strains exhibited high iaa production as evidenced by both colorimetric and high-performance liquid chromatography analysis of culture supernatants. a majority of the strains that produced high amounts of iaa ... | 1998 | 18944847 |
| control of foodborne pathogens and soft-rot bacteria on bell pepper by three strains of bacterial antagonists. | forty-two representative strains of native bacteria associated with fresh peeled baby carrots were isolated and characterized. two of these strains, identified as pseudomonas fluorescens ag3a (pf ag3a) and bacillus yd1, were evaluated in conjunction with another known antagonist, p. fluorescens 2-79 (pf 2-79), for their potential as biocontrol agents of human pathogens (listeria monocytogenes, yersinia enterocolitica, salmonella enterica, and escherichia coli o157:h7) and soft-rot bacteria (erwi ... | 2009 | 19205468 |
| interactions among selected endoparasitic nematodes and three pseudomonads on alfalfa. | meloidogyne hapla, pratylenchus penetrans, and helicotylenchus dihystera, reduced the growth of 'saranac ar alfalfa seedlings when applied at concentrations of 50 nematodes per plant. all except p. penetrans reduced seedling growth when applied at 25 per seedling. m. hapla reduced growth when applied at 12 per seedling. nematodes interacted with three pseudomonads to produce greater growth reductions than were obtained with single pathogens, suggesting synergistic relationships. ditylenchus dips ... | 1982 | 19295682 |
| perturbation of spermine synthase gene expression and transcript profiling provide new insights on the role of the tetraamine spermine in arabidopsis thaliana defense against pseudomonas viridiflava. | the role of the tetraamine spermine in plant defense against pathogens was investigated by using the arabidopsis thaliana-pseudomonas viridiflava pathosystem. the effects of perturbations of plant spermine levels on susceptibility to bacterial infection were evaluated in transgenic plants (35s::spms) that overexpressed the spermine synthase(spms) gene and accumulated spermine, as well as in spms mutants with low spermine levels. the former exhibited higher resistance to p. viridiflava than wild ... | 2011 | 21628628 |
| microbial diversity inside pumpkins: microhabitat-specific communities display a high antagonistic potential against phytopathogens. | recent and substantial yield losses of styrian oil pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) are primarily caused by the ascomycetous fungus didymella bryoniae but bacterial pathogens are frequently involved as well. the diversity of endophytic microbial communities from seeds (spermosphere), roots (endorhiza), flowers (anthosphere), and fruits (carposphere) of three different pumpkin cultivars was studied to develop a biocontrol strategy. a multiphasic approach combining molec ... | 2011 | 21947430 |
| genetic diversity and ecological evaluation of fluorescent pseudomonads isolated from the leaves and roots of potato plants. | a total of 828 isolates of fluorescent pseudomonads (fps) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants grown in different geographical locations in japan, and 16s rrna gene sequences of 776 isolates were successfully determined by direct pcr sequencing. clustering analysis (≥99% identity) identified 13 and 26 operational taxonomic units (otus) for leaf- and root-associated fps, respectively, and 29 otus were identified in the phytosphere of potato plants ... | 2012 | 22791043 |
| a pseudomonas viridiflava-related bacterium causes a dark-reddish spot disease in glycine max. | a virulent pseudomonas viridiflava-related bacterium has been identified as a new pathogen of soybean, one of the most important crops worldwide. the bacterium was recovered from forage soybean leaves with dark-reddish spots, and damage on petioles and pods was also observed. in contrast, common bean was not affected. | 2012 | 22407694 |
| pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level. | the pectinolytic species pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. however, little is known about the molecular evolution of this species. in this study we investigated the intraspecies genetic variation of p. viridiflava amongst local (cretan), as well as international isolates of the pathogen. the genetic and phenotypic variability were investigated by molecular fingerprinting (rep-pcr) and partial sequenc ... | 2012 | 22558343 |
| whole-genome shotgun sequence of pseudomonas viridiflava, a bacterium species pathogenic to ararabidopsis thaliana. | we report here the first whole-genome shotgun sequence of pseudomonas viridiflava strain uasws38, a bacterium species pathogenic to the biological model plant ararabidopsis thaliana but also usable as a biological control agent and thus of great scientific interest for understanding the genetics of plant-microbe interactions. | 2013 | 23405356 |
| efficacy of lactoferricin b in controlling ready-to-eat vegetable spoilage caused by pseudomonas spp. | the microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. the aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. a total of 48 bacterial isolates were collected from ready-to-eat (rte) vegetables and identified as belonging to acinetobacter calcoaceticus, aeromonas media, pseudomonas cichorii, pseudomonas flu ... | 2015 | 26453993 |
| the pseudomonas viridiflava phylogroups in the p. syringae species complex are characterized by genetic variability and phenotypic plasticity of pathogenicity-related traits. | as a species complex, pseudomonas syringae exists in both agriculture and natural aquatic habitats. p.viridiflava, a member of this complex, has been reported to be phenotypically largely homogenous. we characterized strains from different habitats, selected based on their genetic similarity to previously described p.viridiflava strains. we revealed two distinct phylogroups and two different kinds of variability in phenotypic traits and genomic content. the strains exhibited phase variation in p ... | 2014 | 24612372 |
| high-quality draft genome sequence of an endophytic pseudomonas viridiflava strain with herbicidal properties against its host, the weed lepidium draba l. | here, we report the draft genome sequence of pseudomonas viridiflava strain cdrtc14 a pectinolytic bacterium showing herbicidal activity, isolated from the root of lepidium draba l. growing as a weed in an austrian vineyard. the availability of this genome sequence allows us to investigate the genetic basis of plant-microbe interactions. | 2016 | 27795282 |
| pyrene biodegradation enhancement potential of lipopeptide biosurfactant produced by paenibacillus dendritiformis cn5 strain. | effect of biosurfactant on biodegradation of pyrene was studied using a microbial consortium predominantly composed of pseudomonas viridiflava (49.5%) and pseudomonas nitroreducens (32.5%) in a batch experiment containing lipopeptidic biosurfactant, produced by paenibacillus dendritiformis cn5 strain, and mineral salt medium. the results showed that the lipopeptide at 600 and 300mgl(-1) enhanced pyrene degradation to 83.5% and 67% respectively in 24days compared to 16% degradation in its absence ... | 2017 | 27627697 |
| mutability in pseudomonas viridiflava as a programmed balance between antibiotic resistance and pathogenicity. | mutable bacterial cells are defective in their dna repair system and often have a phenotype different from that of their wild-type counterparts. in human bacterial pathogens, the mutable and hypermutable phenotypes are often associated with general antibiotic resistance. here, we quantified the occurrence of mutable cells in pseudomonas viridiflava, a phytopathogenic bacterium in the p. syringae complex with a broad host range and capacity to live as a saprophyte. two phenotypic variants (transp ... | 2015 | 25649542 |
| role of arginine decarboxylase (adc) in arabidopsis thaliana defence against the pathogenic bacterium pseudomonas viridiflava. | polyamine biosynthesis starts with putrescine production through the decarboxylation of arginine or ornithine. in arabidopsis thaliana, putrescine is synthesised exclusively by arginine decarboxylase (adc), which exists as two isoforms (adc1 and 2) that are differentially regulated by abiotic stimuli, but their role in defence against pathogens has not been studied in depth. this work analysed the participation of adc in arabidopsis defence against pseudomonas viridiflava. adc activity and expre ... | 2015 | 25409942 |
| thermospermine catabolism increases arabidopsis thaliana resistance to pseudomonas viridiflava. | this work investigated the roles of the tetraamine thermospermine (tspm) by analysing its contribution to arabidopsis basal defence against the biotrophic bacterium pseudomonas viridiflava. the participation of polyamine oxidases (paos) in tspm homeostasis and tspm-mediated defence was also investigated. exogenous supply of tspm, as well as ectopic expression of the tspm biosynthetic gene acl5, increased arabidopsis col-0 resistance to p. viridiflava, while null acl5 mutants were less resistant ... | 2013 | 23382552 |
| epiphytic survival of pseudomonas viridiflava on tomato and selected weed species. | a rifampicin-nalidixic acid mutant of pseudomonas viridiflava (pv) was studied in the field and greenhouse with respect to its epiphytic survival on the roots and foliage of a susceptible (fm 6203) and resistant (ontario 7710) tomato cultivar and 16 weed species. in the field, populations varied between years, which was attributed to differences in environmental conditions. hot, dry conditions caused rapid decline or elimination of populations. some hosts were more conducive than others in promo ... | 1993 | 24189988 |
| complete genome sequence of pseudomonas viridiflava cfbp 1590, isolated from diseased cherry in france. | pseudomonas viridiflava causes foliar and stem necrosis, as well as stem and root rot on a wide range of plants. we report here the first complete genome of a p. viridiflava strain, isolated from diseased tissue of a cherry tree. | 2017 | 28751394 |