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rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ...19921537892
isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-pcr, 16s rrna gene sequencing, and pulsed-field gel electrophoresis of rochalimaea quintana from a patient with bacillary angiomatosis.rochalimaea quintana was isolated from the blood of a french human immunodeficiency virus-infected patient with bacillary angiomatosis. the isolate showed the typical growth characteristics of rochalimaea species and was inert when typical biochemical testing was used. the purpose of the present work was to characterize and compare this new isolate with reference strains of r. quintana, rochalimaea vinsonii, and rochalimaea henselae by using immunofluorescence, sodium dodecyl sulfate-polyacrylam ...19947519628
proposed tests for the routine identification of rochalimaea species.a study was conducted to establish tests for the routine identification of rochalimaea species. strains used were reference strains of rochalimaea vinsonii and rochalimaea quintana, and a type strain and six human isolates of rochalimaea henselae. rochalimaea species were confirmed to be gram-negative, oxidase-negative, non-motile, urease-negative, indole-negative, catalase-negative, glucose-nonfermenting organisms which failed to grow on macconkey agar. further testing of the organisms in a com ...19937694852
endocarditis in a dog due to infection with a novel bartonella subspecies.vegetative valvular endocarditis involving the aortic and, to a lesser extent, mitral valves was diagnosed echocardiographically in a 3-year-old spayed female labrador retriever. historically, the dog had been treated with tetracycline hydrochloride and prednisolone for positive seroreactivity to ehrlichia canis and antinuclear antigens. although three aerobic and anaerobic blood cultures failed to grow bacteria, blood cultured simultaneously by the lysis centrifugation technique grew a fastidio ...19957699033
polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from rochalimaea species for subtyping.restriction endonuclease analysis of a polymerase chain reaction-amplified dna fragment which included the spacer region between the genes coding for 16s and 23s rrnas and a portion of the gene coding for 23s rrna (spacer + 23s) was done on 10 previously characterized clinical isolates of rochalimaea henselae, one clinical isolate of rochalimaea quintana, and the type strains of r. henselae, r. quintana, rochalimaea vinsonii, and bartonella bacilliformis. brucella abortus dna was not amplified b ...19938102375
proposals to unify the genera bartonella and rochalimaea, with descriptions of bartonella quintana comb. nov., bartonella vinsonii comb. nov., bartonella henselae comb. nov., and bartonella elizabethae comb. nov., and to remove the family bartonellaceae from the order rickettsiales.dna hybridization data (hydroxyapatite method, 50 to 70 degrees c) indicate that rickettsia prowazekii, the type species of the type genus of the family rickettsiaceae, is substantially less closely related to rochalimaea species than was previously thought. the levels of relatedness of rickettsia prowazekii to rochalimaea species and to bartonella bacilliformis under optimal conditions for dna reassociation were 0 to 14%, with 25.5% or greater divergence in related sequences. when stringent rea ...19938240958
antimicrobial susceptibility of rochalimaea quintana, rochalimaea vinsonii, and the newly recognized rochalimaea henselae.the susceptibility of an isolate of rochalimaea quintana, rochalimaea vinsonii and the newly recognized rochalimaea henselae to antimicrobial agents was determined by dilution in mueller-hinton agar supplemented with 5% sheep blood. two inocula of 10(2) and 10(4) cfu were used and tests were read after incubation at 5 and 7 days at 37 degrees c in an atmosphere enriched to 5% carbon dioxide. each isolate was susceptible to amoxycillin, third-generation cephalosporins, tetracyclines, macrolides a ...19938288500
bartonella vinsonii subsp. berkhoffii subsp. nov., isolated from dogs; bartonella vinsonii subsp. vinsonii; and emended description of bartonella vinsonii.two bacterial strains, one isolated from the blood of a dog with valvular endocarditis and one isolated from the blood of a healthy dog, were similar to bartonella species, as determined by a number of phenotypic criteria, including growth characteristics, biochemical reactions, and cell wall fatty acid composition. the results of 16s rrna gene sequence similarity studies confirmed that these strains are closely related and belong in the genus bartonella and that bartonella vinsonii is their clo ...19968782679
epidemiologic evaluation of the risk factors associated with exposure and seroreactivity to bartonella vinsonii in dogs.to determine seroprevalence to bartonella vinsonii subsp berkhoffii in a population of sick dogs from north carolina and virginia and to evaluate potential risk factors associated with increased likelihood of exposure to the organism.19979140552
cosegregation of a novel bartonella species with borrelia burgdorferi and babesia microti in peromyscus leucopus.during surveillance for various tickborne pathogens in the upper midwest during the summer and early fall of 1995, a bartonella-like agent was detected in the blood of mice that were concurrently infected with borrelia burgdorferi or babesia microti (or both). the organism was isolated in pure culture after inoculation of blood from wild-caught mice into c.b-17 scid/scid mice. phylogenetic analysis of the 16s rrna and the citrate synthase genes showed that the novel bartonella species and a bart ...19989466529
a survey of tick-borne bacteria and protozoa in naturally exposed dogs from israel.antibody reactivity against seven bacterial or protozoal pathogens was measured in sera derived from 40 dogs suspected of a tick-borne disease. sera from 73% (29/40) of the dogs reacted with three or more test antigens. seroreactivity was most prevalent to babesia canis antigen (90%) followed by babesia gibsoni (75%), ehrlichia canis (63%), rickettsia conorii--moroccan strain (58%), rickettsia conorii--israeli strain no. 2 (28%), borrelia burgdorferi (10%) or bartonella vinsonii (berkhoffii) (10 ...19989561701
sequential evaluation of dogs naturally infected with ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, ehrlichia ewingii, or bartonella vinsonii.historically, disease manifestations in dogs seroreactive to ehrlichia canis antigens by indirect immunofluorescent antibody testing have been attributed to infection with either e. canis or ehrlichia ewingii. a 1996 study by dawson and colleagues provided pcr evidence that healthy dogs from southeastern virginia could be naturally infected with ehrlichia chaffeensis. this observation stimulated us to determine which ehrlichia spp. infected sick dogs that were referred to our hospital from the s ...19989705408
isolation of a new subspecies, bartonella vinsonii subsp. arupensis, from a cattle rancher: identity with isolates found in conjunction with borrelia burgdorferi and babesia microti among naturally infected mice.bacteremia with fever due to a novel subspecies of bartonella vinsonii was found in a cattle rancher. the subspecies shared major characteristics of the genus bartonella in terms of most biochemical features and cellular fatty acid profile, but it was distinguishable from other subspecies of b. vinsonii by good growth on heart infusion agar supplemented with x factor and by its pattern of enzymatic hydrolysis of peptide substrates. dna relatedness studies verified that the isolate belonged to th ...199910405408
coinfection with multiple tick-borne pathogens in a walker hound kennel in north carolina.both dogs and humans can be coinfected with various ehrlichia, bartonella, rickettsia, and babesia species. we investigated a kennel of sick walker hounds and their owners in southeastern north carolina for evidence of tick-borne infections and associated risk factors. a high degree of coinfection was documented in the dog population. of the 27 dogs, 26 were seroreactive to an ehrlichia sp., 16 to babesia canis, and 25 to bartonella vinsonii, and 22 seroconverted to rickettsia rickettsii antigen ...199910405413
seroepidemiology of bartonella vinsonii subsp. berkhoffii infection in california coyotes, 1994-1998.the prevalence of antibodies to bartonella vinsonii subsp. berkhoffii in coyotes (canis latrans) in california ranged from 51% in central to 34% in southern and 7% in northern california. seropositive coyotes were more likely to be from coastal than inland counties (p clustered distribution of bartonella seropositivity in coyotes suggests that b. vinsonii subsp. berkhoffii infection is vectorborne. further investigation is warranted to evaluate which arthropods are vectors and what the mode of t ...199910511529
bartonella vinsonii subsp. berkhoffii and related members of the alpha subdivision of the proteobacteria in dogs with cardiac arrhythmias, endocarditis, or myocarditis.cardiac arrhythmias, endocarditis, or myocarditis was identified in 12 dogs, of which 11 were seroreactive to bartonella vinsonii subspecies berkhoffii antigens. historical abnormalities were highly variable but frequently included substantial weight loss, syncope, collapse, or sudden death. fever was an infrequently detected abnormality. cardiac disease was diagnosed following an illness of short duration in most dogs, but a protracted illness of at least 6 months' duration was reported for fou ...199910523564
granulomatous disease associated with bartonella infection in 2 dogs.shortly after removal of an engorged tick from the left ear, a 4-year-old greyhound was referred for evaluation of fever and a rapidly enlarging mass in the region of the left submandibular lymph node. histopathologic evaluation of the lymph node resulted in a diagnosis of severe granulomatous lymphadenitis. an 11-year-old mixed-breed dog was referred for evaluation of a 6-week history of serous nasal discharge. histologic examination of a surgical biopsy from a nasal mass indicated multifocal g ...200010668815
bartonella vinsonii subsp. berkhoffii as an agent of afebrile blood culture-negative endocarditis in a human.we report a case of endocarditis in a human infected with bartonella vinsonii subsp. berkhoffii, which causes bacteremia and endocarditis in dogs. bacterial identification was established by pcr amplification and sequencing of an intergenic spacer region (its1), 16s ribosomal dna, and a gene encoding citrate synthase (glta). bartonella antibodies were detected by immunofluorescence.200010747175
cyclic cd8+ lymphopenia in dogs experimentally infected with bartonella vinsonii subsp. berkhoffii.until recently, it was presumed that bartonella vinsonii only infected voles, a species of north american rodents. in april of 1993, however, our laboratory isolated a novel subspecies of b. vinsonii (b. vinsonii subsp. berkhoffii) from the blood of a dog diagnosed with vegetative valvular endocarditis. subsequently, based on a seroepidemiologic survey of dogs from north carolina and virginia presenting for a variety of medical problems, we found evidence supporting a potentially important assoc ...200010889298
coyotes (canis latrans) as the reservoir for a human pathogenic bartonella sp.: molecular epidemiology of bartonella vinsonii subsp. berkhoffii infection in coyotes from central coastal california.bartonella vinsonii subsp. berkhoffii was originally isolated from a dog suffering infectious endocarditis and was recently identified as a zoonotic agent causing human endocarditis. following the coyote bite of a child who developed clinical signs compatible with bartonella infection in santa clara county, calif., this epidemiological study was conducted. among 109 coyotes (canis latrans) from central coastal california, 31 animals (28%) were found to be bacteremic with b. vinsonii subsp. berkh ...200011060089
phylogenetic position of bartonella vinsonii subsp. arupensis based on 16s rdna and glta gene sequences.the nucleotide sequences of the genes encoding 16s rrna and citrate synthase (glta) from a recently described member of the genus bartonella, bartonella vinsonii subsp. arupensis, isolated from mice and from the blood of a patient suffering from bacteraemia, were determined and compared with sequences of the 16s rdna and glta genes of other members of the genus bartonella in order to determine its relative phylogenetic position. b. vinsonii subsp. arupensis appeared to be closely related to b. v ...200111211257
assessing the association between the geographic distribution of deer ticks and seropositivity rates to various tick-transmitted disease organisms in dogs.to determine whether the geographic distribution of deer ticks (ixodes scapularis) was associated with the distribution of dogs seropositive for various tick-transmitted disease organisms (ie, borrelia burgdorferi, rickettsia rickettsii, the human granulocytic ehrlichiosis [hge] agent, ehrlichia canis, and bartonella vinsonii subsp berkhoffii).200111318358
molecular phylogeny of the genus bartonella: what is the current knowledge?species of the genus bartonella are involved in an increasing variety of human diseases. in addition to the 14 currently recognized species, several bartonella strains have been recovered from a wide range of wild and domestic mammals in europe and america. such a high diversity of geographic distributions, animal reservoirs, arthropod vectors and pathogenic properties makes clarification of our knowledge about the phylogeny of bartonella species necessary. phylogenetic data have been inferred m ...200111410341
seroepidemiology of bartonella vinsonii subsp berkhoffii exposure among healthy dogs.to determine seroprevalence of antibodies to bartonella vinsonii subsp berkhoffii and risk factors for seropositivity among working dogs owned by the us government.200111518174
serologic and molecular evidence of coinfection with multiple vector-borne pathogens in dogs from thailand.forty-nine dogs from thailand were evaluated for serologic evidence of exposure or polymerase chain reaction (pcr) evidence of infection with vectorborne pathogens, including ehrlichia sp. (ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, and ehrlichia risticii), bartonella vinsonii subsp. berkhoffi (bvb), spotted fever group (sfg) rickettsiae (rickettsia rickettsii), typhus group (tg) rickettsiae (rickettsia canada, rickettsia prowazekii, and rickettsia typhi), and babesia sp. (babesia c ...200111596732
immunopathology of bartonella vinsonii (berkhoffii) in experimentally infected dogs.following natural infection with bartonella, dogs and humans develop comparable disease manifestations including endocarditis, peliosis hepatis, and granulomatous disease. as the immunologic response to infection in these hosts has not been clearly established, data presented here was derived from the experimental infection of six specific pathogen free (spf) beagles with a known pathogenic strain of bartonella. six dogs were inoculated intravenously with 10(9)cfu of b. vinsonii ssp. berkhoffii ...200111730925
visceral leishmaniasis in a new york foxhound kennel.although endemic throughout much of the world, autochthonous visceral leishmaniasis has been reported on only 3 previous occasions in north america. after diagnosis of visceral leishmaniasis in 4 foxhounds from a kennel in dutchess county, new york (index kennel), serum and ethylenediamine-tetraacetic acid (edta)-anticoagulated blood were collected from the remaining 108 american or cross-bred foxhounds in the index kennel and from 30 beagles and basset hounds that were periodically housed in th ...200211822802
phylogenetic classification of bartonella species by comparing groel sequences.bartonella is a bacterial genus classified in the alpha-proteobacteria on the basis of 165 rdna sequence comparison. the highly conserved heat-shock chaperonin protein, groel, has proved to be a valuable resolving tool to classify ten bartonella species. the groel gene was amplified and sequenced from ten bartonella isolates: bartonella alsatica, bartonella vinsonii subsp. arupensis, bartonella taylorii, bartonella tribocorum, bartonella birtlesii, bartonella henselae marseille (urlly8), b. hens ...200211837299
genetic classification and differentiation of bartonella species based on comparison of partial ftsz gene sequences.currently, 19 species are recognized in the genus bartonella, 7 of which are involved in an increasing variety of human diseases. development of molecular tools for detection, identification, and subtyping of strains and isolates has promoted research on bartonella spp. we amplified and sequenced the portion of the ftsz gene encoding the n-terminal region of the cell division protein for 13 bartonella species: bartonella alsatica, b. birtlesii, b. doshiae, b. elizabethae, b. grahami, b. koehlera ...200212354859
spatial analysis of yersinia pestis and bartonella vinsonii subsp. berkhoffii seroprevalence in california coyotes (canis latrans).zoonotic transmission of sylvatic plague caused by yersinia pestis occurs in california, usa. human infections with various bartonella species have been reported recently. coyotes (canis latrans) are ubiquitous throughout california and can become infected with both bacterial agents, making the species useful for surveillance purposes. this study examined the geographic distribution of 863 coyotes tested for y. pestis and bartonella vinsonii subsp. berkhoffii serologic status to gain insight int ...200312507856
new world origins for haemoparasites infecting united kingdom grey squirrels (sciurus carolinensis), as revealed by phylogenetic analysis of bartonella infecting squirrel populations in england and the united states.phylogenetic analyses of bartonella have suggested divergence between bartonellae that infect mammals native to the old and new worlds. we characterized bartonella isolated from eastern grey squirrels (sciurius carolinensis) in the united states and from grey and red squirrels (sciurus vulgaris) in the united kingdom by nucleotide sequence comparison (glta and groel). isolates from grey squirrels in the united states and the united kingdom were identical, and most similar to bartonella vinsonii, ...200212558350
molecular characterization of the sucb gene encoding the immunogenic dihydrolipoamide succinyltransferase protein of bartonella vinsonii subsp. berkhoffii and bartonella quintana.members of the genus bartonella have historically been connected with human disease, such as cat scratch disease, trench fever, and carrion's disease, and recently have been recognized as emerging pathogens causing other clinical manifestations in humans. however, because little is known about the antigens that elicit antibody production in response to bartonella infections, this project was undertaken to identify and molecularly characterize these immunogens. immunologic screening of a bartonel ...200312874367
the immunologic response of dogs to bartonella vinsonii subspecies berkhoffii antigens: as assessed by western immunoblot analysis.bartonella vinsonii subspecies berkhoffii is a recently recognized zoonotic pathogen that causes endocarditis, granulomatous rhinitis, and granulomatous lymphadenitis in dogs. isolation of b. vinsonii (berkhoffii) from blood or tissue samples is frequently unsuccessful; therefore, diagnosis is primarily dependent on serologic or molecular testing modalities. because previous canine serologic studies have used an indirect immunofluorescence assay (ifa), without western immunoblot (wi) confirmatio ...200312918816
concurrent bartonellosis and babesiosis in a dog with persistent thrombocytopenia.a 12-year-old castrated male west highland white terrier was referred because of recurrent episodes of collapsing. the dog was mildly anemic and severely thrombocytopenic and had high serum alanine aminotransferase activity. infection with bartonella vinsonii (berkhoffii) was initially diagnosed on the basis of serologic testing. despite treatment with a series of antimicrobials and prolonged use of immunosuppressive drugs, thrombocytopenia persisted. after 5 months of treatment, babesia canis o ...200314621218
bartonella vinsonii subspecies berkhoffi as a possible cause of anterior uveitis and choroiditis in a dog.a 2-year old, neutered, female spaniel mixed breed was referred to the north carolina state university veterinary teaching hospital for evaluation of bilateral anterior uveitis. the dog was febrile and, in addition to anterior uveitis, multifocal hyporeflective lesions were present in the tapetal fundus of both eyes. the antibody titer for bartonella vinsonii subspecies berkhoffi was positive (1 : 512). aqueous paracentesis was performed for pcr in an attempt to detect b. vinsonii in the eye but ...200314641826
antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens.the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ...200414765731
a prospective study of canine infective endocarditis in northern california (1999-2001): emergence of bartonella as a prevalent etiologic agent.a prospective study was performed (june 1999 to may 2001) to determine the incidence of infective endocarditis (ie) due to bartonella in dogs in northern california and to compare these patients with other dogs with ie. ie was diagnosed antemortem based on clinical signs and echocardiography in 18 dogs. the etiologic agent was bartonella sp. in 5 dogs (28%) and was diagnosed by high seroreactivity to bartonella (titer > 1:512; range, 1:1,024-1:4,096); and confirmed postmortem by positive polymer ...200414765733
clinicopathological abnormalities and treatment response in 24 dogs seroreactive to bartonella vinsonii (berkhoffii) antigens.bartonella vinsonii (b. vinsonii) subspecies berkhoffii is a recently recognized cause of endocarditis, myocarditis, and granulomatous disease in dogs. in an effort to elucidate other potential disease manifestations, the case records of 24 dogs that were seroreactive to b. vinsonii (berkhoffii) antigens were studied retrospectively. diagnoses included immune-mediated hemolytic anemia, neutrophilic or granulomatous meningoencephalitis, neutrophilic polyarthritis, cutaneous vasculitis, and uveiti ...200415007043
chronic canine ehrlichiosis (ehrlichia canis): a retrospective study of 19 natural cases.nineteen dogs from greece with chronic ehrlichiosis were studied. the dogs exhibited bicytopenia or pancytopenia, bone marrow hypoplasia, seroreactivity to ehrlichia canis (e. canis) antigens, and had no history of drug or radiation exposure. anorexia, depression, severe bleeding tendencies, hypoalbuminemia, and increased serum alanine aminotransferase activity were also hallmarks of the disease. all these animals eventually died, irrespective of the treatment applied. some dogs were also serolo ...200415131097
infection and blood transfusion: a guide to donor screening.in recent years, blood-component therapy has become more accessible in veterinary practice. as with human medicine, care must be taken to minimize the risk of disease transmission from donor to recipient. determining the appropriate diseases to screen for is complicated by regional variations in disease incidence, the existence of chronic carrier states for some diseases, the difficulty in screening-test selection, and testing cost. the feline diseases considered include retroviral infections, f ...200415179926
bartonella henselae igg antibodies are prevalent in dogs from southeastern usa.in contrast to the large body of literature regarding bartonella henselae in humans and cats, there is little information about b. henselae as an infectious agent in dogs. due to the paucity of information regarding the b. henselae serology in dogs, we performed a cross-sectional serosurvey using b. henselae antigen in order to compare the seroprevalence between sick and healthy dogs from the south-eastern usa. ninety-nine sera were collected from clinically healthy dogs. three hundred and one s ...200415369661
the bartonella henselae sucb gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease.bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. a recombinant clone expressing an immunoreactive antigen of b. henselae was isolated by screening a genomic dna cosmid library by western blotting with sera pooled from patients positive for b. henselae igg antibodies by indirect immunofluorescence (ifa). the deduced amino acid sequence of the 43.7 kda encoded protein was found to be 76.3 % identic ...200415585501
bartonella vinsonii subsp. arupensis as an agent of blood culture-negative endocarditis in a human.we report the case of a patient hospitalized with endocarditis. the etiological diagnosis of bartonella was suggested by detection of high titers of antibodies by immunofluorescence and western blotting. two different nested pcrs performed on sera identified bartonella vinsonii subsp. arupensis by sequencing.200515695714
the bartonella vinsonii subsp. arupensis immunodominant surface antigen brpa gene, encoding a 382-kilodalton protein composed of repetitive sequences, is a member of a multigene family conserved among bartonella species.bartonella proteins that elicit an antibody response during an infection are poorly defined; therefore, to characterize antigens recognized by the host, a bartonella genomic expression library was screened with serum from an infected mouse. this process led to the discovery of a bartonella vinsonii subsp. arupensis gene encoding a 382-kda protein, part of a gene family encoding large proteins, each containing multiple regions of repetitive segments. the genes were termed brpa to -c (bartonella r ...200515845521
seroprevalence of antibodies against bartonella species and evaluation of risk factors and clinical signs associated with seropositivity in dogs.to determine the seroprevalence of antibodies against bartonella spp in a population of sick dogs from northern california and identify potential risk factors and clinical signs associated with seropositivity.200515900951
environmental factors associated with bartonella vinsonii subsp. berkhoffii seropositivity in free-ranging coyotes from northern california.bartonella vinsonii subsp. berkhoffii is a newly recognized pathogen of domestic dogs and humans. coyotes (canis latrans) are considered an important reservoir of this bacterium in the western united states, but its vectors are still unknown. our objective was to identify environmental factors associated with bartonella antibody prevalence in 239 coyotes from northern california, using an enzyme-linked immunosorbent assay. in addition, associations were evaluated between b. v. berkhoffii and two ...200516011426
isolation of bacteriophages from bartonella vinsonii subsp. berkhoffii and the characterization of pap31 gene sequences from bacterial and phage dna.bacteriophages enhance bacterial survival, facilitate bacterial adaptation to new environmental conditions, assist in the adaptation to a new host species, and enhance bacterial evasion or inactivation of host defense mechanisms. we describe the detection and purification of a novel tailed bacteriophage from bartonella vinsonii subsp. berkhoffii, which was previously described as a bacteriophage-negative species. we also compare b. vinsonii subsp. berkhoffi pap31 bacteriophage gene sequences to ...200516254445
a bartonella vinsonii berkhoffii typing scheme based upon 16s-23s its and pap31 sequences from dog, coyote, gray fox, and human isolates.since the isolation of bartonella vinsonii subspecies berkhoffii from a dog with endocarditis in 1993, this organism has emerged as an important pathogen in dogs and as an emerging pathogen in people. current evidence indicates that coyotes, dogs and gray foxes potentially serve as reservoir hosts. based upon sequence differences within the 16s-23s its region and pap31 gene, we propose a classification scheme that divides b. vinsonii subsp. berkhoffii isolates into four distinct types. two conse ...200616460911
a serological study of exposure to arthropod-borne pathogens in dogs from northeastern spain.there is limited information regarding the prevalence of many vector borne pathogens in europe and especially in spanish dogs. we investigated 206 sick and 260 clinically healthy dogs from three different regions in northeastern spain for antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap), bartonella henselae (bh), bartonella vinsonii subsp. berkhoffii (bvb), leishmania infantum (li) and borrelia burgdorferi (bb) and for antigen of dirofilaria immitis (di ...200616472522
antibodies to bartonella vinsonii subsp. berkhoffii in moroccan dogs.bartonella vinsonii subsp. berkhoffii is a fastidious microorganism that has been recognized as an emerging human and canine pathogen. we report for the first time on the prevalence of antibodies to b. vinsonii subsp. berkhoffii in domestic dogs from morocco. the overall seroprevalence was 38% (56 of 147 dogs tested). most of the seropositive dogs were stray dogs from rabat (36%, 8 of 22) and khenifra (47%, 47 of 101). antibodies against b. vinsonii subsp. berkhoffii were found infrequently amon ...200616474074
[study on bartonella vinsonii berkhoffii isolated from blood of native dogs in china].to isolate and identify bartonella strains from native dogs in shandong province in china.200616875539
the low seroprevalence of tick-transmitted agents of disease in dogs from southern ontario and quebec.infectious diseases caused by pathogens transmitted by ticks and other insect vectors are an important cause of morbidity and mortality in both dogs and humans throughout north america. the purpose of this study was to determine the seroprevalence of selected vector-transmitted pathogens in southern ontario and quebec. samples submitted to the vector borne disease diagnostic laboratory (vbddl) at the north carolina state university college of veterinary medicine were evaluated for antibodies to ...200617217089
[molecular genetic methods of typing of the bartonellae].the primer systems for the pcr detection of four house-keeping genes of bartonellae in clinical material were developed and tested. the tactics of the species rflp typing was also developed and tested. the scheme of the species rflp typing of bartonellae was tested using as an example two strains for the first time isolated in russia from patients with endocarditis and fever of uncertain origin. the results of the typing were supported by sequencing of the amplicons obtained. according to the se ...200717354603
canine bartonellosis: serological and molecular prevalence in brazil and evidence of co-infection with bartonella henselae and bartonella vinsonii subsp. berkhoffii.the purpose of this study was to determine the serological and molecular prevalence of bartonella spp. infection in a sick dog population from brazil. at the são paulo state university veterinary teaching hospital in botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. antibodies to bartonella henselae and bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. using 16s-2 ...200717583666
association of bartonella with the fleas (siphonaptera) of rodents and bats using molecular techniques.bartonella spp. are putatively vector-borne bacterial agents of humans and animals. fleas have been incriminated as vectors of bartonella spp. and are suspected of transmitting bartonella of rodents and bats, but some of these bartonella spp. have not yet been directly detected in wild caught fleas. we report the molecular detection of bartonella tribocorum, bartonella vinsonii subsp. vinsonii, and two novel genotypes of bartonella from the fleas xenopsylla cheopis, ctenophthalmus pseudagyrtes, ...200717633432
bartonella vinsonii subsp. berkhoffii endocarditis in a dog from saskatchewan.a dog referred for lameness was diagnosed with culture-negative endocarditis. antibodies to bartonella spp. were detected. antibiotic treatment resulted in transient clinical improvement, but the dog developed cardiac failure and was euthanized. bartonella vinsonii subsp. berkhoffii genotype iv was identified within the aortic heart valve lesions by pcr amplification and dna sequencing.200717824328
spatial analysis of the exposure of dogs in rural north-coastal california to vectorborne pathogens.between 0 and 50 per cent of the dogs in eight rural villages in far northern california with a high risk of tickborne diseases were seropositive for anaplasma phagocytophilum and bartonella vinsonii subspecies berkhoffii, and between 0 and 10 per cent were seropositive for borrelia burgdorferi. the odds ratio for the co-exposure of individual dogs to b vinsonii berkhoffii and a phagocytophilum was 18.2. none of the diseases was associated with the sex of the dogs, whether they slept out of door ...200717993656
prevalence of ehrlichia canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, bartonella vinsonii berkhoffii, and rickettsia spp. in dogs from grenada.to identify the tick-borne pathogens in dogs from grenada, we conducted a serologic survey for ehrlichia canis in 2004 (104 dogs) and a comprehensive serologic and molecular survey for a variety of tick-borne pathogens in 2006 (73 dogs). in 2004 and 2006, 44 and 32 dogs (42.3% and 43.8%) were seropositive for e. canis, respectively. in 2006, several tick-borne pathogens were identified by serology and pcr. dna of e. canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, and bartonella ...200818160223
bartonella dna in the blood and lymph nodes of golden retrievers with lymphoma and in healthy controls.although lymphoma is the most common neoplastic process reported in dogs, its precise etiology is unknown. golden retrievers are more likely to develop lymphoma, suggesting a breed predisposition; however, other factors, including environment, immunity, and infection, are likely contributors to oncogenesis.200818289294
molecular characterization of bartonella vinsonii subsp. berkhoffii genotype iii.the molecular characterization of a bartonella vinsonii subsp. berkhoffii genotype iii strain (ncsu strain 06-co1) isolated from the blood of a military working dog diagnosed with endocarditis is reported in this study. several genes were amplified and sequenced for comparative sequence similarity with other strains.200818367567
molecular evidence for bartonella spp. in cat and dog fleas from germany and france.nine hundred and fifty-two fleas were collected from 148 cats and 133 dogs at 18 widely distributed geographic locations in germany and france and examined for the presence of six different bartonella spp. (bartonella bacilliformis, bartonella clarridgeiae, bartonella elizabethae, bartonella henselae, bartonella quintana, bartonella vinsonii subsp. berkhoffii) by pcr. thirty-five specimens (3.7%) tested positive for either b. henselae (14 positive fleas) or b. clarridgeiae (21 positive fleas). d ...200818489542
failure to identify an association between serologic or molecular evidence of bartonella infection and idiopathic rhinitis in dogs.to determine whether infection with or exposure to bartonella spp was associated with idiopathic rhinitis in dogs.200818710315
seroprevalence of bartonella spp. in the endangered island fox (urocyon littoralis).bartonella clarridgeiae-like strains, presently b. rochalimae, were isolated in gray foxes (urocyon cinereoargenteus) in mainland california. the objective of this study was to investigate the presence of bartonella infection in the endangered island fox (urocyon littoralis) found only on the channel islands off the californian coast. between 2001 and 2004, 263 serum samples were collected. antibodies against bartonella vinsonii subsp. berkhoffii (bvb) and b. clarridgeiae (bc) were detected usin ...200919058928
isolation or molecular detection of bartonella henselae and bartonella vinsonii subsp. berkhoffii from dogs with idiopathic cavitary effusions. 200919175739
molecular documentation of bartonella infection in dogs in greece and italy.this study reports the occurrence of "bartonella rochalimae" in europe and the presence of bartonella vinsonii subsp. berkhoffii genotypes ii and iii in dogs in southern italy and provides dna sequencing evidence of a potentially new bartonella sp. infecting dogs in greece and italy.200919261798
isolation of bartonella vinsonii subsp. berkhoffii genotype ii from a boy with epithelioid hemangioendothelioma and a dog with hemangiopericytoma.in this report, we describe isolation of bartonella vinsonii subsp. berkhoffii genotype ii from a boy with epithelioid hemangioendothelioma and a dog with hemangiopericytoma. these results suggest that b. vinsonii subsp. berkhoffii may cause vasoproliferative lesions in both humans and dogs.200919369441
serological evidence of bartonella vinsonii lymphadenopathies in a child bitten by a dog. 200919374641
a confusing case of canine vector-borne disease: clinical signs and progression in a dog co-infected with ehrlichia canis and bartonella vinsonii ssp. berkhoffii.abstract : bartonella spp. are important pathogens in human and veterinary medicine, and bartonellosis is considered as an emerging zoonosis that is being reported with increasing frequency. of 22 known species and subspecies of bartonella, seven have been isolated from dogs, causing disease manifestations similar to those seen in human beings. the wide variety of clinical signs and the possible chronic progression of disease manifestations are illustrated in the case of an infected labrador ret ...200919426442
high prevalence of tick-borne pathogens in dogs from an indian reservation in northeastern arizona.we evaluated the serological and molecular prevalence of selected organisms in 145 dogs during late spring (may/june) of 2005 and in 88 dogs during winter (february) of 2007 from the hopi indian reservation. additionally, in 2005, 442 ticks attached to dogs were collected and identified as rhipicephalus sanguineus. infection with or exposure to at least one organism was detected in 69% and 66% of the dogs in may/june 2005 and february 2007, respectively. exposure to spotted fever group (sfg) ric ...201019469667
bartonella endocarditis: a pathology shared by animal reservoirsand patients.bartonellae were first recognized to cause endocarditis in humans in 1993 when cases caused by bartonella quintana, b. elizabethae, and b. henselae were reported. since the first isolation of bartonella vinsonii subspecies berkhoffii from a dog with endocarditis, this organism has emerged as an important pathogen in dogs and an emerging pathogen in people. subsequently, four types of b. vinsonii subsp. berkhoffii have been described, all of which have been associated with endocarditis in dogs. a ...200919538271
co-isolation of bartonella henselae and bartonella vinsonii subsp. berkhoffii from blood, joint and subcutaneous seroma fluids from two naturally infected dogs.this report describes the clinical presentation, isolation and treatment of two dogs naturally infected with bartonella henselae and bartonella vinsonii subsp. berkhoffii. chronic and progressive polyarthritis was the primary complaint for dog #1, from which b. henselae and b. vinsonii subsp. berkhoffii were cultured on three independent occasions from blood and joint fluid samples, despite administration of nearly 4 months of non-consecutive antibiotic therapy. a clinically atypical and progres ...200919560291
recurrent osteomyelitis in a cat due to infection with bartonella vinsonii subsp. berkhoffii genotype ii. 200919709358
bartonella sp. infection of voles trapped from an interior alaskan site where ticks are absent.to determine whether bartonella species may perpetuate in sites where ticks are absent, we analyzed blood and spleen samples from small mammals trapped near cantwell, alaska during september 1999. blood smears from seven of 48 myodes (clethrionomys) rutilus suggested a bartonella species infection; five of the seven yielded cultures consistent with bartonella species; pcr of rrs, glta, and rpob genes confirmed bartonella infection in four of these culture samples. phylogenetic analysis of rrs, g ...201020090030
evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for ehrlichia, anaplasma, spotted fever group rickettsia, bartonella, and borrelia species in canine neurological diseases (109 cases).vector-transmitted microorganisms in the genera ehrlichia, anaplasma, rickettsia, bartonella, and borrelia are commonly suspected in dogs with meningoencephalomyelitis (mem), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (csf) of dogs with mem is unknown.201020102497
detection and genetic diversity of bartonella vinsonii subsp. berkhoffii strains isolated from dogs in ankara, turkey.bartonella vinsonii subsp. berkhoffii has been identified as an important pathogen in dogs and is an emerging pathogen in people with zoonotic potential. this study aimed to isolate bartonella spp. in 250 blood samples collected from dogs in the province of ankara, turkey, between october 2006 and march 2007. the typing of the 23 isolates was carried out by pcr for citrate synthase (glta) and the 16s-23s intergenic transcribed spacer (its). the prevalence of bacteremia was 9.2% in 250 samples. a ...201020234114
bacillary angiomatosis in an immunosuppressed dog.abstract a dog being treated with immunosuppressive doses of prednisone and azathioprine for pancytopenia of unknown origin, developed, over a 2-week period, multiple erythematous nodular lesions in the skin including footpads. skin samples revealed lesions identical to those of human bacillary angiomatosis (ba). the nodules were composed of multifocal proliferations of capillaries, each lined by protuberant endothelial cells. the capillary clusters were separated by an oedematous connective tis ...201020374571
bartonella vinsonii subsp. berkhoffii and bartonella henselae bacteremia in a father and daughter with neurological disease.abstract:201020377863
molecular evidence of perinatal transmission of bartonella vinsonii subsp. berkhoffii and bartonella henselae to a child.bartonella vinsonii subsp. berkhoffii, bartonella henselae, or dna of both organisms was amplified and sequenced from blood, enrichment blood cultures, or autopsy tissues from four family members. historical and microbiological results support perinatal transmission of bartonella species in this family. it is of clinical relevance that bartonella spp. may adversely influence human reproductive performance.201020392912
seroprevalence of bartonella vinsonii subsp. berkhoffii in urban and rural dogs in turkey.the seroprevalence of bartonella vinsonii subsp. berkhoffii was investigated in stray urban dogs and shepherd and farm guard dogs from rural areas sampled from 10 provinces of turkey. sera from 855 dogs were examined for the presence of anti-b. vinsonii subsp. berkhoffii antibodies by indirect fluorescent antibody test. overall, 56 (6.6%) of the 855 dogs examined, including 16 (3%) of the 522 stray dogs and 40 (12%) of the 333 rural dogs, were seropositive. this is the first report on prevalence ...201020574140
molecular evidence of bartonella infection in domestic dogs from algeria, north africa, by polymerase chain reaction (pcr).bartonella species are being recognized as important bacterial human and canine pathogens, and are associated with multiple arthropod vectors. bartonella dna extracted from blood samples was obtained from domestic dogs in algiers, algeria. polymerase chain reaction (pcr) and dna sequence analyses of the ftsz gene and the 16s-23s intergenic spacer region (its) were performed. three bartonella species: bartonella vinsonii subsp. berkhoffii, bartonella clarridgeiae, and bartonells elizabethae were ...201020682871
suspected needle stick transmission of bartonella vinsonii subspecies berkhoffii to a veterinarian. 201020695992
pcr amplification of bartonella koehlerae from human blood and enrichment blood cultures.abstract:201020735840
persistent infection or successive reinfection of deer mice with bartonella vinsonii subsp. arupensis.bartonella infections are common in rodents. from 1994 to 2006, longitudinal studies of a rodent community, consisting mainly of deer mice (peromyscus maniculatus), were conducted in southwestern colorado to study hantaviruses. blood samples from deer mice captured one or more times during the period 2003 to 2006 (n = 737) were selected to study bartonellae in deer mice. bartonellae were found to be widely distributed in that population, with an overall prevalence of 82.4% (607/737 mice). no cor ...201121239553
bartonella spp. dna in cardiac tissues from dogs in colorado and wyoming.background: several bartonella species (spp.) have been identified in dogs diagnosed with infectious endocarditis (ie) or myocarditis. objective: to interrogate cardiac tissues of dogs with suspected ie for the presence of bartonella spp. dna of dogs in the rocky mountain states. animals: nine dogs with a clinical diagnosis of endocarditis from january 1990 to june 2008 were included. methods: in this retrospective study, medical records at the veterinary teaching hospital were searched. animals ...201121539606
molecular and serological diagnosis of bartonella infection in 61 dogs from the united states.background: molecular diagnosis of canine bartonellosis can be extremely challenging and often requires the use of an enrichment culture approach followed by pcr amplification of bacterial dna. hypotheses: (1) the use of enrichment culture with pcr will increase molecular detection of bacteremia and will expand the diversity of bartonella species detected. (2) serological testing for bartonella henselae and bartonella vinsonii subsp. berkhoffii does not correlate with documentation of bacteremia ...201121615498
isolation or detection of bartonella vinsonii subspecies berkhoffii and bartonella rochalimae in the endangered island foxes (urocyon littoralis).bartonella rochalimae (b.r.) and bartonella vinsonii subsp. berkhoffii (b.v.b.) have been isolated from gray foxes (urocyon cinereoargenteus) in mainland california and high bartonella seroprevalence was reported in island foxes (u. litorralis), especially from santa cruz and santa rosa islands. as a follow-up study, the objectives were to determine the prevalence of bartonella bacteremia and seropositivity and to identify the bartonella species infecting a convenience sample of 51 island foxes ...201121802869
human seroreactivity against bartonella species in the democratic republic of congo.to assess the presence and identity of bartonella species in a pool of human blood samples from drc congo.201121771478
ecological diversity of bartonella species infection among dogs and their owner in virginia.abstract bartonella species comprise a genus of gram-negative, fastidious, intracellular bacteria that have been implicated in association with an increasing spectrum of disease manifestations in dogs and human patients. in this study, chronic canine and human disease, for which causation was not diagnostically defined, were reported by the breeder of a kennel of doberman pinschers. in addition to other diagnostic tests, serology, polymerase chain reaction, and enrichment blood culture were used ...201121736485
invasion of canine erythrocytes by bartonella vinsonii subsp. berkhoffii.bartonella vinsonii subsp. berkhoffii is a recognized cause of endocarditis in dogs and human patients and has been associated with cardiac arrhythmias, myocarditis, granulomatous lymphadenitis, polyarthritis, and granulomatous rhinitis in dogs. little is known regarding the mode of transmission or cellular localization of this bacteria following infection of a canine host. the aim of the current study was to determine whether erythrocytes may serve as a site of infection by b. vinsonii subsp. b ...201122071179
experimental infection by capillary tube feeding of rhipicephalus sanguineus with bartonella vinsonii subspecies berkhoffii.it has been speculated that ticks may serve as vectors of bartonella species. circumstantial, clinical, epidemiological and serological evidence suggest that b. vinsonii subspecies berkhoffii (b. v. berkhoffii) might be transmitted by rhipicephalus sanguineus. the purpose of the present study was to determine whether adult r. sanguineus ticks can be infected with a b. v. berkhoffii genotype ii isolate via capillary tube feeding and whether the infection can then be transmitted from adult females ...201122062313
comparison of serological and molecular panels for diagnosis of vector-borne diseases in dogs.canine vector-borne diseases (cvbd) are caused by a diverse array of pathogens with varying biological behaviors that result in a wide spectrum of clinical presentations and laboratory abnormalities. for many reasons, the diagnosis of canine vector-borne infectious diseases can be challenging for clinicians. the aim of the present study was to compare cvbd serological and molecular testing as the two most common methodologies used for screening healthy dogs or diagnosing sick dogs in which a vec ...201424670154
prevalence of select vector-borne pathogens in stray and client-owned dogs from algiers.data on the prevalence of vector-borne diseases agents infecting canines in algeria is currently lacking. the purpose of this study is to assess by serological and molecular methods the prevalence of select arthropod borne-bacterial infections in client-owned and stray dogs. antibodies to anaplasma phagocytophilum were the most prevalent at 47.7%, followed by borrelia burgdorferi s.l. at 37.6%, ehrlichia canis at 30.0%, bartonella henselae at 32.4% and bartonella vinsonii subsp. berkhoffii at 27 ...201525638478
regional seroreactivity and vector-borne disease co-exposures in dogs in the united states from 2004-2010: utility of canine surveillance.vector-borne disease (vbd) pathogens remain an emerging health concern for animals and humans throughout the world. surveillance studies of ticks and humans have made substantial contributions to our knowledge of vbd epidemiology trends, but long-term vbd surveillance data of dogs in the united states is limited. this seroreactivity study assessed us temporal and regional trends and co-exposures to anaplasma, babesia, bartonella, borrelia burgdorferi, dirofilaria immitis, ehrlichia spp., and spo ...201425325316
bartonella bacteria in nature: where does population variability end and a species start?the application of new molecular approaches has permitted the differentiation of numerous strains belonging to the genus bartonella and identification of new bartonella species. however, the molecular typing of these organisms should be coupled with studies aimed at defining the biological properties of the newly described species. the long-history of co-adaptation between bartonella(1) bacteria and their mammalian hosts and possibly arthropod vectors provides a unique opportunity for applying t ...201222449771
prevalence and potential risk factors for bartonella infection in tunisian stray dogs.bartonellae are blood-borne and vector-transmitted pathogens, some are zoonotic, which have been reported in several mediterranean countries. transmission from dogs to humans is suspected, but has not been clearly demonstrated. our objectives were to determine the seroprevalence of bartonella henselae, bartonella vinsonii subsp. berkhoffii, bartonella clarridgeiae, and bartonella bovis (as a proxy for candidatus bartonella merieuxii) in stray dogs from tunisia, identify the bartonella species in ...201728346866
experimental infection of cats with afipia felis and various bartonella species or subspecies.based upon prior studies, domestic cats have been shown to be the natural reservoir for bartonella henselae, bartonella clarridgeiae and bartonella koehlerae. however, other bartonella species, such as bartonella vinsonii subsp. berkhoffii, bartonella quintana or bartonella bovis (ex weissii) have been either isolated from or bartonella dna sequences pcr amplified and sequenced. in the late 1980s, before b. henselae was confirmed as the etiological agent of cat scratch disease, afipia felis had ...201424972870
an unmatched case controlled study of clinicopathologic abnormalities in dogs with bartonella infection.we compared clinicopathologic findings in dogs with bartonella infection to bartonella spp. negative dogs suspected of a vector-borne disease. cases (n=47) and controls (n=93) were selected on the basis of positive or negative enrichment culture pcr results, respectively. signalment, clinicopathologic findings and treatments were extracted from medical records. dna sequencing identified bartonella henselae (n=28, 59.6%), bartonella vinsonii subsp. berkhoffii (n=20, 42.6%), bartonella koehlerae ( ...201323683861
bartonella clarridgeiae and bartonella vinsonii subsp. berkhoffii exposure in captive wild canids in brazil.summary wild canids are potential hosts for numerous species of bartonella, yet little research has been done to quantify their infection rates in south america. we sought to investigate bartonella seroprevalence in captive wild canids from 19 zoos in são paulo and mato grosso states, brazil. blood samples were collected from 97 wild canids belonging to four different native species and three european wolves (canis lupus). indirect immunofluorescent antibody testing was performed to detect the p ...201524892580
flea species infesting dogs in florida and bartonella spp. prevalence rates.several bartonella spp. associated with fleas can induce a variety of clinical syndromes in both dogs and humans. however, few studies have investigated the prevalence of bartonella in the blood of dogs and their fleas. the objectives of this study were to determine the genera of fleas infesting shelter dogs in florida, the prevalence of bartonella spp. within the fleas, and the prevalence of bartonella spp. within the blood of healthy dogs from which the fleas were collected. fleas, serum, and ...201424268654
seroepidemiology of bartonella infection in gray foxes from texas.gray foxes (urocyon cinereoargenteus) were shown to be naturally infected with bartonella rochalimae, a bartonella species similar to bartonella clarridgeiae (b.c.), and bartonella vinsonii subspecies berkhoffii (b.v.berkhoffii) in northern california. a serological survey was performed to investigate the presence of bartonella infection in 132 gray foxes from west/central texas. using an immunofluorescence antibody test directed against b.v.berkhoffii and b.c., the antibody prevalence was 50% ( ...201222217178
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