| tyrosine degradation in presumptive identification of peptostreptococcus anaerobius. | a new tyrosine medium was developed and evaluated for the differentiation of peptostreptococcus anaerobius from other anaerobic, gram-positive cocci. the strains included 159 originating from clinical specimens and 13 reference strains received from other workers in the field. only one strain of each species was included in the study from multiple cultures from the same patient. this medium is simple to prepare and can be used in a small clinical laboratory. one hundred seventy-two strains of an ... | 1979 | 379031 |
| capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections. | combinations of bacteria isolated from the root canals of teeth with necrotic pulps and periapical bone destruction were tested for their capacity to induce abscess formation and transmissible infections when inoculated subcutaneously into guinea pigs. transmissible infections could be induced with combinations obtained from teeth with purulent apical inflammation, but not with combinations from symptomless teeth with chronic apical inflammation. all combinations which gave transmissible infecti ... | 1979 | 489126 |
| anaerobic bacteria in late infections following orthopedic surgery. | twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ... | 1979 | 492071 |
| differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products. | gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ... | 1979 | 528680 |
| long-chain fatty acids of peptococci and peptostreptococci. | the long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. the resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into four groups. fifteen strains of peptostreptococcus anaerobius were placed in group i because they had a unique, prominent compound that occurred in the area where a c8 to c10 fatty ac ... | 1976 | 1002830 |
| heterogeneity of surfaces of subgingival bacteria as detected by zeta potential measurements. | porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans (a.a.) are gram-negative bacteria which are implicated in various forms of periodontal disease. the gram-positive peptostreptococcus micros may also play an important role. for investigation of the possible adhesion and colonization mechanisms of these organisms, the charge properties of the outermost layers of bacterial cell surfaces were studied through the measurement of zeta potentials at various ph val ... | 1992 | 1357016 |
| enzymatically active peptostreptococcus magnus: association with site of infection. | fifty-four strains of peptostreptococcus magnus (11 were recovered from abdominal infections, 18 were from nonpuerperal breast abscesses, and 21 were from diabetic foot infections; the type strain and three other strains were from the american type culture collection, rockville, md.) and the type strain of peptostreptococcus micros were tested for their ability to produce various enzymes, including catalase, hippurate hydrolase, serine dehydratase, threonine dehydratase, collagenase, gelatinase, ... | 1992 | 1400997 |
| the endodontic microflora revisited. | the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ... | 1992 | 1479923 |
| susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase. | the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ... | 1992 | 1481764 |
| physico-chemical and structural properties of the surfaces of peptostreptococcus micros and streptococcus mitis as compared to those of mutans streptococci, streptococcus sanguis and streptococcus salivarius. | the surface properties of nine streptococcus mitis and four peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. in addition, elemental surface concentration ratios were obtained via x-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. the s. mitis and p. mi ... | 1992 | 1487734 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| [pharmacokinetic, bacteriological, and clinical studies on panipenem/betamipron in children]. | pharmacokinetic, bacteriological and clinical studies were performed on panipenem/betamipron (papm/bp) in children. the results are summarized as follow: 1. twelve patients with various bacterial infectious diseases were treated with papm/bp. each dose was 20 mg/20 mg/kg, administered 3 times daily, in 30-minute intravenous drip infusion. treatments were continued for 5-22 days. clinical efficacies of papm/bp in 12 patients with bacterial infections (1 with suspected sepsis, 5 with pneumonia, 1 ... | 1992 | 1512926 |
| peptostreptococcus micros in human periodontitis. | peptostreptococcus micros is a recognized pathogen in medical infections, and its association with progressive periodontitis was examined in this study. p. micros was isolated from paper-point subgingival samples on anaerobic enriched blood agar plates and identified on the basis of cellular and colonial morphology and selected biochemical tests. in a cross-sectional study involving 907 people with advanced adult periodontitis, 127 with early-onset periodontitis, and 12 with localized juvenile p ... | 1992 | 1528618 |
| physicochemical and structural investigation of the surfaces of some anaerobic subgingival bacteria. | the surfaces of nine clinical isolates of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and peptostreptococcus micros and that of laboratory strain p. gingivalis w83 were studied by using contact angle measurements, x-ray photoelectron spectroscopy, infrared spectroscopy, microelectrophoresis of whole cells, and transmission electron microscopy of whole and sectioned cells. p. intermedia strains were hydrophilic, as judged from their small water contact a ... | 1992 | 1599251 |
| defective neutrophil function in an insulin-dependent diabetes mellitus patients. a case report. | the objective of this study was to evaluate the polymorphonuclear leukocyte (pmn) function in a poorly controlled adult insulin-dependent diabetic patient (iddm) with severe recurrent periodontitis, while describing the microbiological and clinical findings. chemotaxis, superoxide production, and phagocytosis and killing of porphyromonas (bacteroides) gingivalis by the iddm pmn were evaluated 1 week before treatment relative to a healthy, matched control. these analyses revealed a significant (p ... | 1991 | 1651389 |
| subgingival microflora of advanced periodontitis in the dominican republic. | a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ... | 1991 | 1658290 |
| the microflora of periodontal sites showing active destructive progression. | 20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ... | 1991 | 1752997 |
| [chronic conjunctivitis caused by oral anaerobic germs]. | a patient with chronic, unilateral conjunctivitis is presented. the predominant bacterial flora consisted mainly of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. restriction endonuclease fingerprinting of genomic dna demonstrated that p. intermedia most probably originated from the oral cavity. in the oral cavity this species was recovered from the dorsum of the tongue, the tonsillar area and the periodontal pockets. topical treatment with various antibi ... | 1991 | 1758517 |
| [bacteriological examination of infections in the field of oral surgery]. | etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ... | 1991 | 1791332 |
| comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis. | to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ... | 1991 | 1800380 |
| predominant cultivable flora in pericoronitis. | the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ... | 1991 | 1820571 |
| microbial differences in 2 clinically distinct types of failures of osseointegrated implants. | the microflora associated with osseointegrated implants suspected of failing from infection or trauma were evaluated. of 75 patients examined, 11 demonstrated failing fixtures. 22 of 48 fixtures failed in 6 fully edentulous patients and 10 of 34 failed in 5 partially edentulous patients. the etiology of failure was attributed to infection if there was bleeding, suppuration, pain, high plaque and gingival indices and granulomatous tissue upon surgical removal. traumatic etiology was suspected in ... | 1991 | 1843467 |
| chronic conjunctivitis caused by oral anaerobes and effectively treated with systemic metronidazole plus amoxicillin. | in this study, we report on a case of refractory, unilateral anaerobic conjunctivitis. the predominant anaerobic flora consisted of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. by using the technique of restriction endonuclease fingerprinting of genomic dna, it was shown that the p. intermedia likely originated from the oral cavity. topically applied antibiotics had failed to suppress the infection in the past. successful treatment was achieved after sy ... | 1991 | 1890173 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| reactivity of igg from explant cultures of periapical lesions with implicated microorganisms. | the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ... | 1991 | 1940739 |
| specificity of antibodies present in human periapical lesions. | various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ... | 1991 | 1940740 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography. | the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ... | 1990 | 2131168 |
| recolonization of the subgingival microflora after scaling and root planing in human periodontitis. | the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ... | 1990 | 2213468 |
| the predominant cultivable microbiota of crevicular epithelial cells. | the purpose of the present investigation was to compare the composition of the predominant cultivable microbiota associated with gingival crevicular epithelial cells with that of the unattached microbiota recovered from the same site. samples were taken from 2 diseased sites from 8 periodontal patients, by scraping the epithelial lining of the pocket with a curette. the epithelial cells were separated from the unattached subgingival bacteria by centrifugation in a reduced 50% percoll density gra ... | 1989 | 2628861 |
| development and clinical application of dna probe specific for peptostreptococcus micros. | p. micros is thought to be an important pathogen in the etiology of certain inflammatory lesions, however, the role of this microorganism is uncertain due to the lack of rapid and reliable method to identify this species. the purpose of this study was to develop a dna probe specific for the detection of p. micros in order to evaluate its prevalence in oral infectious lesions. the whole genomic dna from p. micros was partially digested and inserted into the vector puc 13. four recombinant clones ... | 1989 | 2632133 |
| [studies on anaerobic infection in oro-maxillary region--rapid diagnosis by gas-liquid chromatography and antibiotic susceptibilities of anaerobic bacteria]. | subject material for this study was pus collected from patients with purulent inflammation in the oro-maxillary region. direct gas-liquid chromatography (glc) analysis was made, bacterial isolation and identification were carried out, and comparisons were made with results from glc analysis and anaerobic isolates in a pyg medium. in addition, antibiotic susceptibilities of anaerobic bacteria were examined. results 1. anaerobic bacteria were isolated from 85 of 100 cases of obstructive abscesses. ... | 1989 | 2641196 |
| serological studies of peptostreptococci using an indirect fluorescent antibody test. | members of the genus peptostreptococcus are frequent isolates from periodontal lesions. a study was undertaken for determination of the serological relationships of oral and non-oral strains of several species of this genus. eighty-nine strains of peptostreptococci representing seven species were tested by means of an indirect fluorescent antibody technique (ifa), with rabbit antisera to peptostreptococcus anaerobius atcc 27337, peptostreptococcus micros vpi 2618-a, and peptostreptococcus produc ... | 1989 | 2685068 |
| peptostreptococcus micros in polymicrobial abscesses. | | 1988 | 2894531 |
| the growth of oral bacteria on saliva. | the present experiments were aimed at studying the degradation of salivary glycoproteins by the oral microflora. to this end, s. sanguis i strain ny476 and s. sanguis ii (s. mitior) strain ny581 were grown continuously in human-whole saliva. under these conditions, the strains produced a variety of cell-associated hydrolytic activities, including glycosidases, exo- and endopeptidases, and esterases. s. sanguis ii generally exhibited higher levels of enzyme activity than did s. sanguis i, in part ... | 1987 | 3305628 |
| enrichment of subgingival microflora on human serum leading to accumulation of bacteroides species, peptostreptococci and fusobacteria. | this study was undertaken to identify ecological factors that favour opportunistic pathogenic species in the subgingival microflora. in a first approach, human serum as a substitute for gingival exudate, was used for batch-wise enrichment of subgingival plaque. the microflora resulting after 5-6 enrichment steps consisted of black-pigmented and non-black-pigmented bacteroides species, peptostreptococcus micros and fusobacterium nucleatum as the main organisms. it is noted that the same group of ... | 1987 | 3674857 |
| microbiologic analyses of nasal polyp tissue. | nasal polyps from 40 patients were cultured within 2 1/2 hours after surgical removal to determine whether microorganisms were present. the first 20 polyps were cultured for aerobic and anaerobic bacteria, viruses, fungi, mycoplasmas, and mycobacteria. of these 20 polyps, eight were sterile by all tests, one grew cryptococcus albidus, one grew sporobolomyces, one had large numbers of peptostreptococcus micros and propionibacterium acnes, greater than 10(6) colony-forming units per gram (cfu/gm), ... | 1986 | 3722630 |
| peptostreptococcal vertebral osteomyelitis. | the clinical course of a patient with vertebral osteomyelitis caused by peptostreptococcus micros is described. the organism was isolated on two occasions from lumbar vertebral biopsies and was visualized in histologic sections of one of the biopsies. the patient had no apparent infections at other body sites. the infection was eradicated after 6 weeks of intravenous clindamycin therapy. | 1986 | 3771752 |
| isolation and characterization of a mucin-degrading strain of peptostreptococcus from rat intestinal tract. | a mucin-degrading microorganism was isolated from the intestinal tract by serial sectioning from the serosal side of the caecum wall from a conventional rat. the ability of degrading the intestinal water-soluble mucin was present both in vivo after monocontamination of germ-free rats and in vitro, when adding the microbe to mucin medium. the morphology, gram-positive cocci single or in short chains and the very weak biochemical activities allow us to place this strain in the species peptostrepto ... | 1986 | 3788570 |
| survival analysis of periodontal sites before and after periodontal therapy. | periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ... | 1985 | 3860518 |
| peptococcaceae bacteremia. | bacteremia with peptococcaceae is an uncommon clinical manifestation of infection with this family of microorganisms. a 20-month review of 12 patients with bacteremic infections due to anaerobic gram-positive cocci revealed that obstetrical patients during the peripartum period constitute the group at greatest risk for the development of such infections. eight of the 12 patients were young women hospitalized on the obstetrical service. seven patients had postpartum endometritis and one patient h ... | 1986 | 3956135 |
| serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis. | in the present study we sought to determine whether serum antibody was present against microorganisms which predominate in the subgingival flora of young adults with generalized severe periodontitis (sp). subjects with sp were often seropositive for eubacterium brachy, fusobacterium nucleatum e3c22, and peptostreptococcus micros, whereas subjects with juvenile periodontitis (jp) and subjects with healthy periodontium (hp) were not. both sp and jp subjects were more frequently seropositive for ba ... | 1985 | 3988337 |
| anaerobic bacteria in urine before and after prostatic massage of infertile men. | in the present study 25 infertile men delivered urine before and after prostatic massage. expressed prostatic secretion (eps) was obtained from 11 men. aerobic and anaerobic bacterial analyses showed that a total of 33 isolates was found in samples of urine voided before massage as compared to 59 isolates after massage of the prostate. there was an increase in the number of anaerobes whereas there was no change in the number of aerobes. the occurrence of eps did not influence the number of aerob ... | 1985 | 4000063 |
| glycine metabolism. 3. a flavin-linked dehydrogenase associated with the glycine cleavage system in peptococcus glycinophilus. | | 1967 | 4289245 |
| electron transport function of a heat-stable protein and a flavoprotein in the oxidative decarboxylation of glycine by peptococcus glycinophilus. | | 1966 | 4290071 |
| glycine metabolism. lipoic acid as the prosthetic group in the electron transfer protein p2 from peptococcus glycinophilus. | | 1973 | 4588680 |
| total synthesis of acetate from co 2 . v. determination by mass analysis of the different types of acetate formed from 13 co 2 by heterotrophic bacteria. | mass analysis was used to determine the amount of acetate which is totally synthesized from (13)co(2) during fermentations by clostridium formicoaceticum, c. acidiurici, c. cylindrosporum, butyribacterium rettgeri, and diplococcus glycinophilus. in the fermentation of fructose by c. formicoaceticum, 27% of the acetate was found to be totally synthesized from co(2), and the remaining acetate was unlabeled, having been formed from fructose. evidence is presented that the purine-fermenting organism ... | 1972 | 5058447 |
| glycine metabolism. i. properties of the system catalyzing the exchange of bicarbonate with the carboxyl group of glycine in peptococcus glycinophilus. | | 1966 | 5901047 |
| glycine metabolism. ii. kinetic and optical studies on the glycine decarboxylase system from peptococcus glycinophilus. | | 1966 | 5901048 |
| glycine metabolism. iv. effect of borohydride reduction on the pyridoxal phosphate-containing glycine decarboxylase from peptococcus glycinophilus. | | 1967 | 6016614 |
| acute suppurative parotitis associated with anaerobic bacteremia. | most episodes of acute suppurative parotitis are caused by staphylococcus aureus. reported here is a patient who had acute parotitis associated with anaerobic sepsis caused by bacteroides melaninogenicus and peptostreptococcus micros. acute parotitis should be included in the ever-widening spectrum of clinical infections caused by anaerobic bacteria. | 1981 | 6110333 |
| bacteriology of dental abscesses of endodontic origin. | aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. sampling was by syringe aspiration. strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. randomly selected colonies (100) from each culture were purified, characterized, and identified. seventy percent of the bacterial isolates were either strict anaerobes or microaerophilic. o ... | 1983 | 6630460 |
| antibody reactive with peptostreptococcus micros in the sera of patients with periodontal disease. | subgingival plaque samples were cultured for the isolation of peptostreptococcus micros from individuals with and without chronic periodontitis. humoral antibody from each person reacted with p. micros in an indirect fluorescent-antibody test, but not all of the sera reacted in a passive hemolysis test. no correlations were observed between the presence of antibody reactive with p. micros and the isolation of p. micros or the gingival health of the individual. | 1982 | 6749898 |
| bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. | the presence of bacteria in 17 single-rooted teeth, with periapical lesions, was studied throughout a whole period of treatment. the root canals were irrigated with physiologic saline solution during instrumentation. no antibacterial solutions or dressings were used. bacteria were found in all initial specimens from the teeth (median number of bacterial cells 4 x 10(5), range 10(2) - 10(7)) and the number of strains in the specimens ranged from 1 to 10.88% of the strains were anaerobic. the most ... | 1981 | 6947391 |
| streptococcus pneumoniae and three species of anaerobic bacteria in experimental otitis media in guniea pigs. | three species of anaerobic bacteria commonly isolated in upper respiratory tract infections in humans were studied in an animal model of otitis media. the ability of propionibacterium acnes to induce acute otitis media was comparable with that of streptococcus pneumoniae, the classic middle ear pathogen. the response to peptostreptococcus micros was weak and irregular, and no effects of bacteroides asaccharolyticus were observed despite the use of high doses (10(8) colony-forming unite per ear). ... | 1982 | 7086197 |
| demonstration of human immunoglobulin g fc-binding activity in oral bacteria. | nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ... | 1994 | 7496956 |
| a retrospective review of cases of anaerobic empyema and update of bacteriology. | we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ... | 1995 | 7548560 |
| bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use. | the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ... | 1995 | 7548575 |
| microbiologic and clinical study of polytetrafluoroethylene membranes for guided bone regeneration around implants. | this study determined the microbiota of the mucosa- and implant-facing parts of expanded polytetrafluoro ethylene augmentation material, and the influence of major periodontopathogens on the healing process associated with guided bone regeneration around dental implants. seventeen patients with nine dehiscence and eight extraction defects were studied. prior to surgery and at membrane removal, microbial morphotypes, total viable counts, and the occurrence of selected microbial species were exami ... | 1995 | 7615319 |
| periodontal microbiota of mobile and non-mobile teeth. | the mechanism of accelerated periodontal destruction around teeth with occlusal trauma and increased mobility remains unclear. one possibility is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. this study compared the subgingival microflora in mobile and non-mobile teeth of 35 adults on supportive maintenance therapy and 15 with untreated adult periodontitis. in each subject, subgingival paper-point samples were obtained from a mobile tooth ... | 1995 | 7623258 |
| crystalline surface protein of peptostreptococcus anaerobius. | the surface ultrastructure of three anaerobic gram-positive cocci frequently encountered in oral infections, peptostreptococcus micros, p. magnus and p. anaerobius, was studied. the type strains of p. micros (dsm 20468) and p. anaerobius (atcc 27337), several clinical isolates of both species and the type strain of p. magnus (dsm 20470) were included. thin-sectioned cells studied by electron microscopy revealed a homogeneous layer outside the peptidoglycan layer in p. anaerobius. in p. micros an ... | 1995 | 7773401 |
| periodontal findings in spouses. a clinical, radiographic and microbiological study. | clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ... | 1995 | 7775676 |
| microbiota of rapidly progressive periodontitis lesions in association with clinical parameters. | the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ... | 1994 | 7853132 |
| the gingival immune response to periodontal pathogens in juvenile periodontitis. | a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ... | 1994 | 7870467 |
| 16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov. | the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ... | 1994 | 8123556 |
| relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults. | to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ... | 1994 | 8157769 |
| impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis. | the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ... | 1994 | 8195981 |
| [cytotoxicity of anaerobic bacteria isolated from infected root canal]. | bacteria in the infected root canal play an important role in the progression of the periapical lesion. the purpose of the present study was to investigate the cytotoxic effects of bacteria isolated from an infected root canal on the periapical lesion. four obligately anaerobic bacteria were isolated and their sonic extracts (se) were prepared. the cytotoxic assay of each se for mc3t3-e1 cell was used with respect to the morphological cell changes and [3h]-thymidine uptake. the following results ... | 1993 | 8228523 |
| description of two morphotypes of peptostreptococcus micros. | peptostreptococcus micros is often isolated from abscesses in several parts of the human body. the oral cavity is considered the natural habitat for the species, which has been implicated as a periodontal pathogen. in plaque samples from periodontitis patients we observed the presence of a rough morphotype of p. micros in addition to the previously recognized smooth morphotype. the rough morphotype has not been described previously. both morphotypes are frequently isolated simultaneously from th ... | 1993 | 8240959 |
| role of suspected periodontopathogens in microbiological monitoring of periodontitis. | periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. although a specificity to some degree is found for actinobacillus actinomycetemcomitans in localized juvenile periodontitis (ljp), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. what we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshol ... | 1993 | 8260004 |
| bacteriologic findings in peritonsillar abscesses in young adults. | aspirated pus samples from 124 patients with peritonsillar abscess were cultured quantitatively for aerobes and anaerobes. a total of 98% of the samples yielded bacteria. of the 550 isolates obtained (mean, 4.4 per patient), 143 were aerobes (representing 16 species or groups) and 407 were anaerobes (representing 40 species or groups). aerobes were isolated from 86% of patients-alone in 20 cases and together with anaerobes in 87. the most common aerobic isolates were streptococcus pyogenes (isol ... | 1993 | 8324134 |
| oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment. | microbial populations colonizing the teeth are a major source of pathogens responsible for oral and dental infections, including periodontal diseases, gingivitis, pericoronitis, endodontitis, peri-implantitis, and postextraction infections. each entity has distinct clinical and microbial features. bacterial species associated with oral infections include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, campylobacter rectus, eubacterium ... | 1993 | 8324136 |
| sub-gingival microflora in macaca mulatta species of rhesus monkey. | the macaca mulatta species of rhesus monkey is one of several non-human primate (nhp) models for periodontal disease. this report presents the bacteriology of the gingival sulci in m. mulatta monkeys. three sub-gingival sites (maxillary right central incisor, the disto-buccal of the mandibular left second molar and mesio-buccal of the mandibular right second molar) of 9 monkeys were evaluated clinically before scaling and 7 days after scaling. plaque samples were obtained from sub-gingival sites ... | 1993 | 8426283 |
| microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. | a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ... | 1993 | 8463936 |
| peptostreptococcus micros has a uniquely high capacity to form hydrogen sulfide from glutathione. | there are high amounts of hydrogen sulfide in deep periodontal pockets. this volatile sulfur compound may be formed from l-cysteine, but only low levels of this amino acid can be expected to be present in periodontal pockets. glutathione, l-gamma-glutamyl-l-cysteinylglycine, is in high concentration in most tissue cells, and this tripeptide may be more readily available as a source of hydrogen sulfide formation in the pockets. the ability of 37 different species of oral bacteria to utilize gluta ... | 1993 | 8510983 |
| salivary levels of suspected periodontal pathogens in relation to periodontal status and treatment. | the primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. the interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. in the present study, salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, and peptostreptococcus micros were determin ... | 1995 | 8530742 |
| competition for peptides and amino acids among periodontal bacteria. | we recently studied the utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine), l-cysteinylglycine and l-cysteine by anaerobic bacteria. the rate of hydrogen sulfide formation from these compounds was determined and it was concluded that peptostreptococcus micros and fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. in the present study it is shown that methyl mercaptan formation from l-methionine and l-methionyl-containing peptides can also be used to ... | 1995 | 8544102 |
| treatment of peptostreptococcus micros endocarditis with teicoplanin. | | 1995 | 8562764 |
| oral microbiota in subjects with a weak or strong response in experimental gingivitis. | the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ... | 1995 | 8583022 |
| antimicrobial and toxic effects of established and potential root canal irrigants. | three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants peridex, chlorhexidine gluconate (0.12%), and therasol were tested. alcohol and saline served as controls also. the in vitro portion of this study used four different microorganisms (streptococcus mutans, peptostreptococcus micros, prevotella intermedius, and porphyromonas gingivalis) to determine antimicrobial effect. presterilized 7-mm diameter whatman paper disks soaked with t ... | 1995 | 8596073 |
| microbiological features and pathogenesis of peritonsillar abscesses. | samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ... | 1995 | 8605900 |
| utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care. | the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ... | 1996 | 8739166 |
| clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects. | thirteen periodontally healthy subjects were monitored clinically for 6-12 months. clinical measurements at 6-weekly intervals included duplicate pd measurements, presence of plaque, redness, and bleeding on probing. baseline measurements consisted of 2 visits 1 wk apart. microbial samples were taken from 11 of the subjects who had completed at least 8 months of monitoring. levels of serum antibodies to 12 periodontal species were determined from 10 subjects. standard deviations of replicate pd ... | 1996 | 8814590 |
| bacteriologic findings in tonsillitis and pericoronitis. | bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ... | 1996 | 8816129 |
| the dynamics of microbial colonization of barrier membranes for guided tissue regeneration. | the microbial colonization of expanded polytetrafluoroethylene membrane by putative periodontopathogens at 3 minutes of intraoral manipulation was determined in 42 patients with 42 mandibular posterior two- to three-wall defects. twenty patients exhibited no periodontal pockets of > or = 5 mm, other than the study site, and low levels of pathogens (group a). twenty-two patients revealed multiple periodontal pockets of 5 mm or more and numerous pathogens (group b). within the preceding 3 months o ... | 1996 | 8832481 |
| infection of a total knee joint prosthesis by peptostreptococcus micros and propionibacterium acnes in an elderly ra patient: implant salvage with longterm antibiotics and needle aspiration/irrigation. | we describe the favourable long-term outcome after late total knee joint prosthesis infection in an elderly ra patient. peptostreptococcus micros and propionibacterium acnes were cultured from the synovial fluid, and the finding of a coexistent abscess at the root of a wisdom tooth suggested a dental origin to the joint infection. long-term antibiotics in conjunction with aspiration/irrigation resulted in salvage of the implant and an excellent functional outcome. | 1996 | 8853177 |
| molecular analysis of microflora associated with dentoalveolar abscesses. | the microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16s rrna genes were randomly amplified by means of conserved eubacterial primers and cloned. restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16s rrna from the cultured bacteria was used to detect putative unculturable bacteria. clones representative of five predominant groups of uncultured organisms were sequenced. two were identified as porp ... | 1996 | 8904410 |
| degradation of host protease inhibitors and activation of plasminogen by proteolytic enzymes from porphyromonas gingivalis and treponema denticola. | bacterial proteases may participate in the pathogenesis of periodontal diseases through their action on host proteins. in the present study, the ability of selected periodontopathogens, as well as two proteases isolated from porphyromonas gingivalis and treponema denticola, to degrade host protease inhibitors was evaluated. the activation of human plasminogen by the two bacterial proteases was also investigated. proteolytic breakdown of host protease inhibitors (alpha-1-antitrypsin, antichymotry ... | 1996 | 8936322 |
| comparison of activities of trovafloxacin (cp-99,219) and five other agents against 585 anaerobes with use of three media. | agar dilution testing was used to compare the in vitro activities of trovafloxacin and ofloxacin, ciprofloxacin, imipenem, metronidazole, and clindamycin against 585 anaerobes. the activity of trovafloxacin was superior to those of ofloxacin and ciprofloxacin, with 94%, 91%, 96%, 100%, 90%, and 100% inhibition of bacteroides fragilis, non-fragilis bacteroides species, peptostreptococcus magnus/peptostreptococcus micros, clostridium perfringens, prevotella bivia/prevotella disiens, and fusobacter ... | 1996 | 8953106 |
| degradation of lactoferrin by periodontitis-associated bacteria. | the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ... | 1996 | 8961558 |
| development and evaluation of a selective and differential medium for the primary isolation of peptostreptococcus micros. | peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. a selective and differential medium for the primary isolation of p. micros was developed and evaluated. columbia cna agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (p. micros medium: pmm). p. micros has a chara ... | 1996 | 9028263 |
| characterization of smooth and rough morphotypes of peptostreptococcus micros. | isolation of the smooth (sm) morphotype of peptostreptococcus micros, a suspected oral pathogen, is sometimes accompanied by isolation of a rough (rg) morphotype of p. micros. the rg type readily changes to a sm-like variant (rgsm) in broth culture. sm and rg isolates and rgsm variants were compared to determine whether these three types are the result of phase variation. the rgsm variants resembled the sm morphotype in colony morphology; furthermore, the sm type and the rgsm type did not have t ... | 1997 | 9103622 |
| matrix metalloproteinases-1, -3 and -8 and myeloperoxidase in saliva of patients with human immunodeficiency virus infection. | human immunodeficiency virus (hiv)-seropositive patients have frequently severe gingival inflammation and/or attachment loss. in addition many infectious diseases affect their periodontium with varying clinical manifestations. matrix metalloproteinases seem to play a key role in physiological periodontal remodelling and pathological tissue destruction. the aim of the present study was to characterize the presence, molecular forms, cellular sources, activities, and relative amounts of fibroblast- ... | 1996 | 9171509 |
| protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum. | the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ... | 1997 | 9178115 |
| periodontal findings in cohen syndrome with chronic neutropenia. | radiographic periodontal status and microbiological findings of periodontal pockets in subjects with cohen syndrome are presented in this report. this hereditary disorder causes mental retardation, and neutropenia is one feature of the syndrome. fifteen patients with cohen syndrome and 15 controls matched for age and sex and, as far as possible, according to the degree of mental retardation were examined. alveolar bone loss was evaluated from the panoramic radiographs. two subgingival samples we ... | 1997 | 9182743 |
| associations of endodontic symptoms and signs with particular combinations of specific bacteria. | significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ... | 1996 | 9206427 |
| the antimicrobial activity of endodontic sealers to anaerobic bacteria. | the effect of five root canal sealers and two root canal dressing materials on the growth of three anaerobic bacteria associated with endodontic infections was determined using the agar diffusion inhibitory test. samples of the following endodontic sealers (apexit, ketac-endo, roth sealer, sealapex and tubliseal) and root canal dressing materials (pulpdent and root-cal) were incubated for 48 h with the following anaerobic bacteria: capnocytophaga ochracea, porphyromonas gingivalis and peptostrep ... | 1996 | 9206446 |
| occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings. | bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. the purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. dentin ... | 1997 | 9226388 |
| beta-lactamase producing bacteria in adult periodontitis. | in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ... | 1997 | 9266340 |
| increased susceptibility of rag-2 scid mice to dissemination of endodontic infections. | specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. to investigate this question we compared periapical-lesion pathogenesis in rag-2 severe combined immunodeficient (scid) mice with immunocompetent control mice following surgical pulp exposure. in order to equalize the bacterial challenge, an infection protocol ... | 1997 | 9284152 |