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succiniclasticum ruminis gen. nov., sp. nov., a ruminal bacterium converting succinate to propionate as the sole energy-yielding mechanism.a gram-negative, anaerobic, nonmotile, non-spore-forming, rod-shaped bacterium that fermented succinate quantitatively to propionate was isolated from a high dilution of rumen ingesta obtained from a dairy cow fed a production diet containing grass silage as the main roughage source. this organism did not grow on any of the following energy sources: 12 carbohydrates, pyruvate, lactate, 7 dicarboxylic acids, aspartate, citrate, and trans-aconitate. both rumen fluid and yeast extract were necessar ...19957537062
succinispira mobilis gen. nov., sp. nov., a succinate-decarboxylating anaerobic bacterium.a succinate-decarboxylating anaerobic bacterium, designated strain 19gly1t, was previously isolated from a mixed culture growing with glycolate. the almost complete sequence of the 16s rrna gene (1495 nt) was determined for this strain. on the basis of 16s rrna gene sequence homology, strain 19gly1t was identified as a member of the sporomusa sub-branch of the 'low g+c' gram-positive bacteria. phylogenetic analysis showed that strain 19gly1t was most closely related to succiniclasticum ruminis, ...199910425757
extensive set of 16s rrna-based probes for detection of bacteria in human feces.for the detection of six groups of anaerobic bacteria in human feces, we designed seven new 16s rrna-based oligonucleotide probes. this set of probes extends the current set of probes and gives more data on the composition of the human gut flora. probes were designed for phascolarctobacterium and relatives (phasco741), veillonella (veil223), eubacterium hallii and relatives (ehal1469), lachnospira and relatives (lach571), and eubacterium cylindroides and relatives (ecyl387), and two probes were ...200212039758
comparison of the cecal microbiota of domestic and wild turkeys.the extent to which production methods alter intestinal microbial communities of livestock is currently unknown. as the intestinal microbiota may affect animal health, nutrition, and food safety, a baseline comparison of the cecal communities of domestic and wild turkeys was performed. oligonucleotide fingerprinting of ribosomal rna (rrna) genes (ofrg) of 2,990 16s rrna clones and dot blot quantification of dominant populations were used to identify the dominant bacterial taxa. seventy-three per ...200818183454
human gut bacterial communities are altered by addition of cruciferous vegetables to a controlled fruit- and vegetable-free diet.in the human gut, commensal bacteria metabolize food components that typically serve as energy sources. these components have the potential to influence gut bacterial community composition. cruciferous vegetables, such as broccoli and cabbage, contain distinctive compounds that can be utilized by gut bacteria. for example, glucosinolates can be hydrolyzed by certain bacteria, and dietary fibers can be fermented by a range of species. we hypothesized that cruciferous vegetable consumption would a ...200919640972
performance of a sulfidogenic bioreactor and bacterial community shifts under different alkalinity levels.the performance of a sulfidogenic bioreactor and the response of bacterial populations to influent alkalinity changes were investigated. the bioreactor reached 40% of sulfate removal efficiency (sre) with 0 mg l(-1) of alkalinity, and single-stranded conformation polymorphism profiles showed that some members of bacteroides, dysgonomonas, sporobacter, quinella, and citrobacter became dominant populations. 16s rrna gene library analysis indicated that the actinobacteria group increased from 0% in ...201020688512
characterization of phascolarctobacterium succinatutens sp. nov., an asaccharolytic, succinate-utilizing bacterium isolated from human feces.isolation, cultivation, and characterization of the intestinal microorganisms are important for understanding the comprehensive physiology of the human gastrointestinal (gi) tract microbiota. here, we isolated two novel bacterial strains, yit 12067(t) and yit 12068, from the feces of healthy human adults. phylogenetic analysis indicated that they belonged to the same species and were most closely related to phascolarctobacterium faecium acm 3679(t), with 91.4% to 91.5% 16s rrna gene sequence sim ...201222081579
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