| present status of the sugarcane mosaic subgroup of potyviruses. | until recently, sugarcane mosaic virus (scmv) was believed to be a single potyvirus consisting of a large number of strains, differing from each other in certain biological and antigenic properties. the use of affinity-purified polyclonal antibodies directed towards the surface-located, virus-specific amino termini of the coat proteins showed that 17 strains from australia and the united states represented four distinct potyviruses, namely johnsongrass mosaic virus (jgmv), maize dwarf mosaic vir ... | 1992 | 1450763 |
| characterisation of potyviruses from sugarcane and maize in china. | sugarcane or maize leaves with mosaic virus symptoms were collected from 13 sites in china. sequence data showed that all 8 samples from maize contained sugarcane mosaic virus (scmv); complete sequences were determined from 2 samples and partial sequences (the ci coding region and the 3'-part of the genome) from the others. the 5 sugarcane samples all contained a virus tentatively described as sorghum mosaic virus (srmv) and in three of them scmv was also detected; 2 srmv sequences and the 3 scm ... | 2002 | 12111432 |
| maize dwarf mosaic disease in different regions of china is caused by sugarcane mosaic virus. | sugarcane mosaic virus (scmv) was detected in all 62 maize samples collected from eight maize-growing provinces in china showing dwarf mosaic symptoms by immunocapture reverse-transcription polymerase chain reaction (rt-pcr). maize dwarf mosaic virus (mdmv), sorghum mosaic virus (srmv) and johnsongrass mosaic virus (jgmv), however, were not detected in any of the samples by rt-pcr. eleven cdna fragments of approximately 0.8 kilobases covering most of the coat protein (cp) gene of scmv were seque ... | 2002 | 12491109 |
| characterization of a distinct johnsongrass mosaic virus strain isolated from sorghum in nigeria. | a virus isolated from sorghum in nigeria has been partially characterized. it was tested by enzyme-linked immunosorbent assay (elisa) using antisera to maize dwarf mosaic virus, johnsongrass mosaic virus (jgmv), sugarcane mosaic virus strain-mdb, sorghum mosaic virus, and zea mosaic virus. a partial host range, symptom phenotypes for selected sorghum lines, and the mass of the coat protein (cp) subunit was analyzed by sodium-dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its ... | 2005 | 15503220 |
| identification of a naturally occurring recombinant isolate of sugarcane mosaic virus causing maize dwarf mosaic disease. | the complete nucleotide sequence of a potyvirus causing severe maize dwarf mosaic disease in shaanxi province, northwestern china was determined (genbank accession no. ay569692). the full genome is 9596 nucleotides in length excluding the 3 '-terminal poly (a) sequence. it contains a large open reading frame (orf) flanked by a 149 nt 5'-untranslated region (utr) and a 255 nt 3'-utr. the putative polyprotein encoded by this large orf comprises of 3063 amino acid residues. sequence comparisons and ... | 2005 | 15744565 |
| identification of prevalent potyvirus on maize and johnsongrass in corn fields of tehran province of iran and a study on some of its properties. | during a growing season in 2004, 231 leaf samples of virus infected and mosaic and dwarf mosaic symptoms showing maize (zea mays l.) plants and 258 leaf samples of mosaic showing johnsongrass (sorghum halepens l.) plants from various corn fields in tehran province were collected. serological tests of das-elisa and diba were performed on samples using antisera of sugarcane mosaic virus (scmv), maize dwarf mosaic virus (mdmv), sorghum mosaic virus (srmv) and johnsongrasss mosaic virus (jgmv). in b ... | 2006 | 17390895 |
| identification and sequence analysis of potyviruses infecting crops in vietnam. | fifty-two virus isolates from 13 distinct potyvirus species infecting crops in vietnam were identified and the 3' region of each genome was sequenced. the viruses were: bean common mosaic virus (bcmv), potato virus y (pvy), sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv), chilli veinal mottle virus (chivmv), zucchini yellow mosaic virus (zymv), leek yellow stripe virus (lymv), shallot yellow stripe virus (sysv), onion yellow dwarf virus (oydv), turnip mosaic virus (tumv), dasheen mosa ... | 2008 | 17906829 |
| the genomic sequence and biological properties of pennisetum mosaic virus, a novel monocot-infecting potyvirus. | the complete nucleotide sequences of two isolates of pennisetum mosaic virus (penmv) were determined. the viral genome comprised 9,611 nucleotides (nt) excluding the 3'-terminal poly(a) sequence, with the capacity of encoding a single polyprotein of 3,065 amino acids. the large open reading frame is flanked by a 172-nt 5'-untranslated region (utr) and a 244-nt 3'-utr. sequence comparisons and phylogenetic analyses of the complete genome and polyproteins suggest that penmv is closely related to o ... | 2008 | 18365128 |
| viruses causing mosaic disease in sugarcane and their genetic diversity in southern china. | a survey of cultivated hybrid sugarcane (saccharum inter-specific hybrid) and noble sugarcane (saccharum officinarum) in southern china for the presence of sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv) and sugarcane streak mosaic virus (scsmv) was conducted by rt-pcr from the years 2003 to 2006. scmv and srmv, but not scsmv, were found. a high incidence of scmv and srmv coinfection was revealed in both hybrid and noble sugarcanes. all coinfected plants showed mosaic symptom, whereas ... | 2008 | 18438601 |
| the complete sequence of a sugarcane mosaic virus isolate causing maize dwarf mosaic disease in china. | the complete sequence of a potyvirus from maize in zhejiang province was determined. the rna was 9596 nucleotides long, excluding the 3'-poly (a) tail, and there was a single long open reading frame (orf) of 9192 nts encoding a 346.1 ku polyprotein. the polyprotein had substantial amino acid sequence homology with those encoded by the rnas of a chinese isolate of sorghum mosaic virus (srmv-c) and a bulgarian isolate of maize dwarf mosaic virus, but it was most closely related to sugarcane mosaic ... | 2002 | 18759055 |
| serological relationships between the cylindrical inclusion proteins of potyviruses. | abstract antisera to the cytoplasmic inclusion proteins (cips) of bean yellow mosaic (bymv), clover yellow vein (clyvv), turnip mosaic (tumv), sweet potato feathery mottle (spfmv), and maize dwarf mosaic (mdmv) potyviruses were used to examine the relationships between the cips of 18 potyviruses. the antisera to cips of bymv, clyvv, tumv, and spfmv cross-reacted to most or all of the purified cips tested in western blot assays. the mdmv cip antiserum reacted significantly only to the mdmv and so ... | 1998 | 18944875 |
| sequence and relationships of sugarcane mosaic and sorghum mosaic virus strains and development of rt-pcr-based rflps for strain discrimination. | abstract sugarcane mosaic is the most widespread virus disease affecting sugarcane production. we have established a collection of seven prominent sugarcane mosaic potyvirus (scmv) strains currently causing disease in sugarcane throughout the world and originally found in sugarcane in the united states. this collection includes scmv strains a, b, d, and e, and the sorghum mosaic virus (srmv) strains sch, sci, and scm. these viruses were propagated on sorghum bicolor cv. rio and purified. cloned ... | 1997 | 18945064 |
| genetic diversity among viruses associated with sugarcane mosaic disease in tucumán, argentina. | sugarcane leaves with mosaic symptoms were collected in 2006--07 in tucumán (argentina) and analyzed by reverse-transcriptase polymerase chain reaction (rt-pcr) restriction fragment length polymorphism (rflp) and sequencing of a fragment of the sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) coat protein (cp) genes. scmv was detected in 96.6% of samples, with 41% showing the rflp profile consistent with strain e. the remaining samples produced eight different profiles that did not ... | 2009 | 19055433 |
| rapid identification of viruses causing sugarcane mosaic by direct sequencing of rt-pcr products from crude extracts: a method for large scale virus surveys. | sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) diversity studies are important to characterize virus populations in sugarcane producing areas, enabling (i) identification of shifts in predominant strains, (ii) detecting associations of strains with specific varieties, and (iii) possibly exposing the appearance of new strains which may affect the performance of varieties in a region. recent studies have shown significant sequence variability within scmv populations around the world ... | 2009 | 19152809 |
| simultaneous detection and identification of four sugarcane viruses by one-step rt-pcr. | sugarcane mosaic disease (smd) caused by the sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv) and sugarcane streak mosaic virus (scsmv) and sugarcane yellow leaf disease (syld) caused by the sugarcane yellow leaf virus (scylv) are the two most prevalent and economically important viral diseases of sugarcane. in this study, a one-step quadruplex reverse transcription (rt)-pcr method that employed virus-specific primers was developed for the simultaneous detection and differentiation of ... | 2009 | 19646484 |
| complete nucleotide sequence and taxonomy of sugarcane streak mosaic virus, member of a novel genus in the family potyviridae. | the complete genomic sequence of a pakistani isolate of sugarcane streak mosaic virus (scsmv-pak) is determined to be 9782 nucleotides in length, excluding the 3' poly(a) tail, and it comprises a large open reading frame encoding a polyprotein of 3130 amino acid residues. the deduced polyprotein is likely to be cleaved at nine putative protease sites by three viral proteases to ten mature proteins. conserved motifs of orthologous proteins of other potyviruses are identified in corresponding posi ... | 2010 | 20162446 |
| iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences. | iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ... | 2017 | 27632283 |
| characterization of a sorghum mosaic virus (srmv) isolate in china. | sorghum mosaic virus (srmv), a causal agent of the destructive sugarcane mosaic disease, has a global presence. an isolate of srmv infecting a commercially-grown sugarcane plant was recovered from the hainan province of china. the virions were visualized by an electron microscope, and the coat proteins (cps) were sequenced by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and tandem mass spectrometry. discrepancies between the cp predicted and actual amino acid sequ ... | 2016 | 26981005 |
| a one-step real-time rt-pcr assay for the detection and quantitation of sugarcane streak mosaic virus. | sugarcane mosaic disease is caused by the sugarcane streak mosaic virus (scsmv; genus poacevirus, family potyviridae) which is common in some asian countries. here, we established a protocol of a one-step real-time quantitative reverse transcription pcr (real-time qrt-pcr) using the taqman probe for the detection of scsmv in sugarcane. primers and probes were designed within the conserved region of the scsmv coat protein (cp) gene sequences. standard single-stranded rna (ssrna) generated by pcr- ... | 2015 | 26185758 |
| transgenic sugarcane resistant to sorghum mosaic virus based on coat protein gene silencing by rna interference. | as one of the critical diseases of sugarcane, sugarcane mosaic disease can lead to serious decline in stalk yield and sucrose content. it is mainly caused by potyvirus sugarcane mosaic virus (scmv) and/or sorghum mosaic virus (srmv), with additional differences in viral strains. rna interference (rnai) is a novel strategy for producing viral resistant plants. in this study, based on multiple sequence alignment conducted on genomic sequences of different strains and isolates of srmv, the conserve ... | 2015 | 25685813 |
| development of a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for the detection of sugarcane mosaic virus and sorghum mosaic virus in sugarcane. | a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detecting sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) in sugarcane. six sets of four primers corresponding to the conserved coat protein gene were designed and tested for each virus. three primer sets designed for detecting scmv and four for detecting srmv were successful in the rt-lamp assay. the effective primer sets were not only specific for their target virus, but also able to ... | 2015 | 25445794 |
| inheritance and segregation of virus and herbicide resistance transgenes in sugarcane. | transgenic sugarcane parents containing multiple copies of herbicide resistance ( bar) and sorghum mosaic virus (srmv) resistance ( hut) genes were crossed with non-transgenic sugarcane varieties. segregation of the transgenes in the progeny was determined using southern blot analysis; herbicide resistance and srmv resistance were assessed using bioassays. the segregation data were used to infer linkage relationships between transgenes in the parent plants. in two of the parents, all transgene i ... | 2002 | 12582639 |
| a sugarcane pathogenesis-related protein, scpr10, plays a positive role in defense responses under sporisorium scitamineum, srmv, sa, and meja stresses. | a pathogenesis-related gene, scpr10 , was isolated from sugarcane and its bio-function was characterized, demonstrating that scpr10 was involved in plant defense responses to sporisorium scitamineum , srmv, sa, and meja stresses. plant fungal and viral diseases are the major concerns in sugarcane industry. many anti-fungal and antivirus components, including pathogenesis-related (pr) proteins, have been identified. the pathogenesis-related protein 10 (pr10) is the dominant group in pr families, ... | 2017 | 28634719 |
| characterization of a novel potyvirus isolated from maize in israel. | a potyvirus (proposed name of zea mosaic virus [zemv]) isolated from maize in israel was analyzed by serology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of capsid proteins, symptomatology, and sequencing. parts of the nuclear inclusion b, coat protein, and 3' regions were sequenced; the amino acid sequence of zemv capsid was determined by time-of-flight mass spectrometry (tofms). the results of these analyses were compared with those of similar analyses of the followin ... | 2000 | 18944557 |
| molecular characterization of a distinct potyvirus from whitegrass in china. | apotyvirus isolated from perennial whitegrass ( pennisetum centrasiaticum tzvel.) in north china was characterized at the molecular level. the 3' terminal nucleotide (nt) sequence of 1669 nt of the viral rna genome has been determined, which covered the coding region of the c-terminal part of the large nuclear inclusion protein (nib, rna polymerase), capsid protein (cp) gene and the 3' nontranslated region (ntr). the cp gene consisted of 909 nt (including the stop codon) encoding 302 amino acid ... | 2003 | 12756626 |