| complete nucleotide sequence of a strawberry isolate of beet pseudoyellows virus. | in our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after rt-pcr using primer sets representing multiple regions of strawberry pallidosis associated virus (spav) genome and failed to react with antibodies directed against the recombinant coat protein (cp) of spav. dsrna extracted from this plant showed a similar pattern to that of spav indicating that another crinivirus may infect strawberry. sequence ... | 2004 | 15266105 |
| further variability within the genus crinivirus, as revealed by determination of the complete rna genome sequence of cucurbit yellow stunting disorder virus. | the complete nucleotide (nt) sequences of genomic rnas 1 and 2 of cucurbit yellow stunting disorder virus (cysdv) were determined for the spanish isolate cysdv-allm. rna1 is 9123 nt long and contains at least five open reading frames (orfs). computer-assisted analyses identified papain-like protease, methyltransferase, rna helicase and rna-dependent rna polymerase domains in the first two orfs of rna1. this is the first study on the sequences of rna1 from cysdv. rna2 is 7976 nt long and contains ... | 2003 | 12917477 |
| nucleotide sequence and genome organization of cucumber yellows virus, a member of the genus crinivirus. | the genome of cucumber yellows virus (cuyv), isolated in japan from cucumber (cucumis sativus l.), was completely sequenced and shown to be bipartite. cuyv rna1 consisted of 7889 nucleotides and encompassed seven open reading frames (orfs), which is typical of the closteroviridae, including a heat-shock protein 70 homologue, a coat protein and a diverged coat protein (cpd). cuyv rna2 consisted of 7607 nucleotides and included two orfs: orf1a potentially encoded a polyprotein containing putative ... | 2003 | 12655104 |
| analysis of the rna of potato yellow vein virus: evidence for a tripartite genome and conserved 3'-terminal structures among members of the genus crinivirus. | double-stranded rna preparations produced from potato plants graft-inoculated with a peruvian isolate of potato yellow vein virus (pyvv; genus crinivirus, family closteroviridae) contain five rna species denoted rna 1, rna 2, rna 3, x and y of approximately 8, 5.3, 3.8, 2.0 and 1.8 kbp, respectively. the complete nucleotide sequences of pyvv rnas 1, 2 and 3 and northern hybridization analysis showed that pyvv rna 1 contained the replication module and an additional open reading frame (p7), while ... | 2004 | 15218192 |