| reducing agents interfere with the detection of lettuce necrotic yellows virus in infected plants by immunoblotting with monoclonal antibodies. | a procedure is described for the detection of lettuce necrotic yellows virus (lnyv) nucleocapsid protein (n) or envelope glycoprotein (g) by immuno-blotting with their respective monoclonal antibodies. the antigens can be detected in 1-10 mg of fresh tissue from systemically infected nicotiana glutinosa leaves showing prominent symptoms. the composition of the extraction buffer played a crucial role in the recovery of antigenically active proteins. procedures involving tissue extraction in the p ... | 1990 | 2370289 |
| the multiplication of lettuce necrotic yellows virus. | | 1971 | 4109519 |
| rna-dependent rna polymerase associated with particles of lettuce necrotic yellows virus. | | 1972 | 4110279 |
| infectious nucleocapsid particles of lettuce necrotic yellows virus with rna-dependent rna polymerase activity. | | 1972 | 4117126 |
| some properties of lettuce necrotic yellows virus rna and its in vitro transcription by virion-associated transcriptase. | | 1973 | 4725630 |
| structure of lettuce necrotic yellows virus. i. electron microscopy of negatively stained preparations. | | 1967 | 4861247 |
| inactivation of lettuce necrotic yellows virus by chelating agents. | | 1969 | 4975943 |
| inactivation of lettuce necrotic yellows virus by ultraviolet irradiation. | | 1969 | 5764085 |
| localization of lettuce necrotic yellows virus in host leaf tissue. | | 1965 | 5850880 |
| factors affecting the local lesion response of nicotiana glutinosa to lettuce necrotic yellows virus. | | 1967 | 6017398 |
| purification of lettuce necrotic yellows virus by column chromatography on calcium phosphate gel. | | 1967 | 6022688 |
| genomic organization of lettuce necrotic yellows rhabdovirus. | we have mapped the genome of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of the family rhabdoviridae. we have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded rna genome of lnyv. the lnyv genome appears to contain six genes, the five expected genes coding for the virion proteins, and a sixth gene of unknown function, as for sonchus yellow net virus (synv), a member of the genus nucleorhabdo ... | 1994 | 8178430 |
| complete nucleotide sequence of a putative new cytorhabdovirus infecting lettuce. | the full-length nucleotide sequence of the genomic rna of a new cytorhabdovirus infecting lettuce was determined. six open reading frames were found in the antigenomic sequence of the 12,926-nt negative-sense viral rna genome. the genomic organisation was similar to that of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus: 3'-n-p-3-m-g-l-5', where n is the capsid protein gene, p the putative phosphoprotein gene, 3 a gene coding for a putative protein of unknown ... | 2008 | 17943394 |
| localization and recovery of lettuce necrotic yellows virus from xylem tissues of nicotiana glutinosa. | | 1966 | 18611525 |
| enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes. | viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ... | 2013 | 24314645 |
| functional analysis of a weak viral rna silencing suppressor using two gfp variants as silencing inducers. | rna silencing in plants can be triggered by the introduction of an exogenous gene. green fluorescent protein (gfp) has been widely used as a visual reporter to study rna silencing and viral-mediated suppression of rna silencing in the model plant nicotiana benthamiana. in transgenic n. benthamiana plants expressing an endoplasmic reticulum targeted gfp variant (16c) known as mgfp5, rna silencing can be induced by ectopic over-expression of mgfp5. however, other gfp variants can also be used to i ... | 2017 | 27836657 |
| cytorhabdovirus p protein suppresses risc-mediated cleavage and rna silencing amplification in planta. | plant viruses have evolved to undermine the rna silencing pathway by expressing suppressor protein(s) that interfere with one or more key components of this antiviral defense. here we show that the recently identified rna silencing suppressor (rss) of lettuce necrotic yellows virus (lnyv), phosphoprotein p, binds to rna silencing machinery proteins ago1, ago2, ago4, rdr6 and sgs3 in protein-protein interaction assays when transiently expressed. in planta, we demonstrate that lnyv p inhibits mirn ... | 2016 | 26808923 |
| structure of the c-terminal domain of lettuce necrotic yellows virus phosphoprotein. | lettuce necrotic yellows virus (lnyv) is a prototype of the plant-adapted cytorhabdoviruses. through a meta-prediction of disorder, we localized a folded c-terminal domain in the amino acid sequence of its phosphoprotein. this domain consists of an autonomous folding unit that is monomeric in solution. its structure, solved by x-ray crystallography, reveals a lollipop-shaped structure comprising five helices. the structure is different from that of the corresponding domains of other rhabdovirida ... | 2013 | 23785215 |
| genetic characterization of novel putative rhabdovirus and dsrna virus from japanese persimmon. | deep-sequencing analysis of nucleic acids from leaf tissue of japanese persimmon trees exhibiting fruit apex disorder in some fruits detected two molecules that were graft transmitted to healthy seedlings. one of the complete genomes consisted of 13 467 nt and encoded six genes similar to those of plant rhabdoviruses. the virus formed a distinct cluster in the genus cytorhabdovirus with lettuce necrotic yellows virus, lettuce yellow mottle virus and strawberry crinkle virus in a phylogenetic tre ... | 2013 | 23704275 |
| lettuce necrotic yellows cytorhabdovirus protein localization and interaction map and comparison with nucleorhabdoviruses. | lettuce necrotic yellows virus (lnyv), sonchus yellow net virus (synv) and potato yellow dwarf virus (pydv) are members of the rhabdoviridae family that infect plants. lnyv is a cytorhabdovirus that replicates in the cytoplasm, while synv and pydv are nucleorhabdoviruses that replicate in the nuclei of infected cells. lnyv and synv share a similar genome organization with a gene order of nucleoprotein (n), phosphoprotein (p), putative movement protein (mv), matrix protein (m), glycoprotein (g) a ... | 2011 | 22190014 |
| strawberry crinkle virus, a cytorhabdovirus needing more attention from virologists. | summary taxonomic relationship: a member of nonsegmented, negative-sense, single-stranded rna viruses of the genus cytorhabdovirus (type member: lettuce necrotic yellows virus), family rhabdoviridae, order mononegavirales. members of the family rhabdoviridae can infect vertebrates, invertebrates and plants. physical properties: virions are bacilliform, 74-88 nm in diameter and 163-383 nm in length with surface projections probably composed of trimers of the glycoprotein g, occurring in the cytop ... | 2000 | 20572980 |
| completion of the genome sequence of lettuce necrotic yellows virus, type species of the genus cytorhabdovirus. | we completed the genome sequence of lettuce necrotic yellows virus (lnyv) by determining the nucleotide sequences of the 4a (putative phosphoprotein), 4b, m (matrix protein), g (glycoprotein) and l (polymerase) genes. the genome consists of 12,807 nucleotides and encodes six genes in the order 3' leader-n-4a(p)-4b-m-g-l-5' trailer. sequences were derived from clones of a cdna library from lnyv genomic rna and from fragments amplified using reverse transcription-polymerase chain reaction. the 4a ... | 2006 | 16313992 |
| nucleocapsid gene variability reveals two subgroups of lettuce necrotic yellows virus. | the complete nucleocapsid (n) genes of eight australian isolates of lettuce necrotic yellows virus (lnyv) were amplified by reverse transcription pcr, cloned and sequenced. phylogenetic analyses of these sequences revealed two distinct subgroups of lnyv isolates. nucleotide sequences within each subgroup were more than 96% identical but heterogeneity between groups was about 20% at the nucleotide sequence level. however, less than 4% heterogeneity was noted at the amino acid level, indicating mo ... | 2005 | 15824884 |
| complete nucleotide sequence of northern cereal mosaic virus and its genome organization. | we determined the complete nucleotide sequence, 13, 222 nucleotides (nts) of the northern cereal mosaic virus (ncmv). the genome had 273 nt 5' trailer sequence and 90nts 3' leader sequence. it formed a terminal complementarity in 25 nts of both terminal sequences. a characteristic intergenic sequence (consensus) separating genes, 3'-auucuuuuugacucuagu-5' was presented. the genome had nine open reading frames (orfs) on the viral complementary sequence. five putative proteins of ncmv were postulat ... | 2000 | 10963343 |
| detection of dna and rna plant viruses by pcr and rt-pcr using a rapid virus release protocol without tissue homogenization. | a simple, single-step plant tissue preparation protocol suitable for the detection of viruses by the polymerase chain reaction and reverse transcription-polymerase chain reaction is described. the effect of buffer components and ph, and the incubation temperature for the release of virus from plant material was evaluated. a small amount of plant tissue was heated in a solution containing 100 mm tris-hcl, ph 7.4 or 8.4, 1 m kcl and 10 mm edta for 10 min at 95 degrees c and the supernatant used fo ... | 1995 | 8530569 |
| structure of the nucleocapsid protein gene of rice yellow stunt rhabdovirus. | the complete nucleotide sequence of the n protein gene of rice yellow stunt rhabdovirus (rysv) was determined by sequencing of cdna clones derived from the viral genomic rna. the 3' end of the n gene (messenger sense) was defined by sequence analysis of cdna clones generated from the n protein mrna by 3'race. the 5' end sequence of the gene was putatively assigned as 5'-aacac-3'; this sequence is found in the presumed 3' leader/n gene junction region. the mrna encoding the rysv n protein is 1714 ... | 1994 | 8091667 |
| analysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus. | the complete nucleotide sequence of the nucleocapsid (n) gene of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of plant rhabdoviruses, has been determined from cdna clones of both the viral genomic and messenger rnas. the previously identified n gene was adjacent to the 3' leader rna and began at position 91 from the 3' end of the lnyv genome. analysis of the sequence showed that the n mrna contained a 78-nt 5' untranslated region, followed by a 1377-nt open ... | 1994 | 8030210 |
| protein composition of the virions of five plant rhabdoviruses. | five plant rhabdoviruses fall into two groups based on the patterns produced by their dissociated virion proteins when separated electrophoretically. lettuce necrotic yellows virus (lnvy) and sonchus virus (sv) virions contained only one m protein and ns protein. sowthistle yellow vein virus (syvv), sonchus yellow net virus (synv) and eggplant mottled dwarf virus (emdv) virions contained two m proteins and no detectable ns protein. the m and g proteins of lnyv and sv were easily released from th ... | 1981 | 7327907 |
| serological analysis of lettuce necrotic yellows virus preparations by immunodiffusion. | | 1971 | 5543833 |
| loss of ribosomes in nicotiana glutinosa l. infected with lettuce necrotic yellows virus. | | 1970 | 5424815 |
| structure of lettuce necrotic yellows virus. ii. electron microscopic studies on the effect of ph of phosphotungstic acid stain on the morphology of the virus. | | 1969 | 4180628 |
| analysis of lettuce necrotic yellows virus structural proteins with monoclonal antibodies and concanavalin a. | three major structural proteins of lettuce necrotic yellow virus (lnyv) were identified by discontinuous polyacrylamide gel electrophoresis (page) to have mr approximately 78,000 (g), 57,000 (n), and 19,000 (m). unreduced g and m proteins had faster mobilities in page indicating the presence of disulfide bonds. the g protein was shown to be glycosylated with a complex network of oligosaccharides containing beta-n-acetylchitobiose n-linked to asparagine residues of the protein. up to 17 additiona ... | 1988 | 2972113 |
| cytorhabdovirus phosphoprotein shows rna silencing suppressor activity in plants, but not in insect cells. | rna silencing in plants and insects provides an antiviral defense and as a countermeasure most viruses encode rna silencing suppressors (rss). for the family rhabdoviridae, no detailed functional rss studies have been reported in plant hosts and insect vectors. in agroinfiltrated nicotiana benthamiana leaves we show for the first time for a cytorhabdovirus, lettuce necrotic yellows virus (lnyv), that one of the nucleocapsid core proteins, phosphoprotein (p) has relatively weak local rss activity ... | 2015 | 25591176 |
| diversity and evolutionary history of lettuce necrotic yellows virus in australia and new zealand. | lettuce necrotic yellows virus (lnyv) is the type member of the genus cytorhabdovirus, family rhabdoviridae, and causes a severe disease of lettuce (lactuca sativa l.). this virus has been described as endemic to australia and new zealand, with sporadic reports of a similar virus in europe. genetic variability studies of plant-infecting rhabdoviruses are scarce. we have extended a previous study on the variability of the lnyv nucleocapsid gene, comparing sequences from isolates sampled from both ... | 2016 | 26526146 |
| cytorhabdovirus p3 genes encode 30k-like cell-to-cell movement proteins. | plant viruses encode movement proteins (mp) to facilitate cell-to-cell transport through plasmodesmata. in this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (tvcv) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (lnyv) and alfalfa dwarf virus (adv)) that their p3 proteins function as mp similar to the tvcv p30 protein. all three mp localized to plasmodesmata when ectopically expressed. in addition, we show ... | 2016 | 26700068 |