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echinococcus vogeli in man, with a review of polycystic hydatid disease in colombia and neighboring countries.three cases of polycystic hydatid disease (phd) from colombia are reported and 11 others from the region are reviewed. when cysts from two patients were fed to a dog and an ocelot about 250 mature and gravid specimens of echinococcus vogeli and two poorly developed strobilae, respectively, were recovered. these human cases constitute the first record of the larval stage of e. vogeli, previously known only from the strobilar stage in the type host, the bush dog (speothos venaticus). based on the ...1979572148
evidence for a fel d i-like molecule in the "big cats" (felidae species).in this study, we investigated the cross-reactivity pattern of ige and igg4 antibodies to the major feline allergen, fel d i. we studied the ige and igg4 response of 11 cat-allergic patients against fel d i-like structures in eight members of the felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. hair from these "big cats" was collected, extracted, and used in a rast system and histamine-release test. by means of a rast-inhibition assay with affinity-p ...19901695231
hepatozoon sp. in wild carnivores in texas.twelve coyotes (canis latrans), three bobcats (lynx rufus) and six ocelots (felis pardalis) from the gulf coast of texas were infected with hepatozoon sp. the geographic distribution of infected wild animals coincides with the highest prevalence of hepatozoon canis infection in domestic dogs for which the wild species may act as a reservoir.19883411720
a coprological survey of parasites of wild neotropical felidae.in conjunction with an ecological study of jaguars in the cockscomb basin of belize, central america, fecal samples from jaguars (panthera onca), jaguarundis (felis yagouaroundi), ocelots (felix pardalis), and pumas (felix concolor) were examined for parasite products (eggs, larvae, and oocysts). of the 45 samples examined, 39 (86.7%) were positive for parasite products, 23 of 25 (92%) jaguar samples were positive, as were all of the puma (4/4) and ocelot (8/8) samples. four of 6 samples from un ...19863783346
[vaccination of rabbits and fur animals].short survey of vaccination programmes for rabbits and fur animals and of vaccines, available in western germany. rabbits: myxomatosis, pasteurella infection, dysenteria; mustelides (mink, polecat a.o.): distemper, virus enteritis, botulism, pseudomonas infection; fox: leptospirosis, distemper, fox encephalitis; ocelot: panleukopenia; chinchilla: pseudomonas infection, coli infection, yersinia infection.19853992574
discovery of lagochilascaris sp. in the larynx of a costa rican ocelot (felis paradalis mearnsi). 19725078604
echinococcus infections in colombian animals.the results of a survey involving more than 4,000 colombian mammals, carried out to detect echinococcus infections, are presented. adult worms were found in 5 of 121 carnivores: e. oligarthrus in 1 of 11 ocelots, 2 of 9 jaguarundi cats and single puma; and e. vogeli in 1 of 15 domestic dogs. although bush dogs were present, none could be examined. polycystic larvae were found in 96 of 325 pacas (29.5%) and in 6 of 1,168 (0.5%) spiny rats, proechimys spp. none of the 118 agouti showed hydatids bu ...19817325284
fine specificity of b-cell epitopes on felis domesticus allergen i (fel d i): effect of reduction and alkylation or deglycosylation on fel d i structure and antibody binding.the repertoire of b-cell epitopes on the major cat allergen, fel d i, was analyzed with monoclonal antibodies (moabs) in topographic mapping studies and in immunoassays with antigen derived from other cat (felidae) species. four essentially nonoverlapping epitopes on fel d i, designated fd1a to d, were defined by use of 15 anti fel d i moabs in cross-inhibition radioimmunoassay. only moabs directed against epitope fd1b bound to putative fel d i homologues in hair and dander extracts from seven o ...19947508462
ige and igg4 binding to the ocelot variant of the cat (felis domesticus) major allergen fel d i. 19947522418
comparative anatomy of the cardiac foramen ovale in cats (felidae), dogs (canidae), bears (ursidae) and hyaenas (hyaenidae).the structure of the foramen ovale from 16 species representing 4 carnivore families, the felidae, canidae, ursidae and hyaenidae, was studied using the scanning electron microscope. the felidae were represented by 9 domestic cat fetuses (felis catus), 2 snow leopard neonates (uncia uncia), an ocelot neonate (leopardus pardalis), 2 lion neonates (panthera leo), a panther neonate (panthera pardus) and 3 tigers (neofelis tigris), comprising 2 fetuses and a neonate. the canidae were represented by ...19957649822
delayed hypersensitivity test with paracoccidioidin in captive latin american wild mammals.the aim of this investigation was to study epidemiological aspects of paracoccidioidomycosis, the main endemic systemic mycosis in brazil. this study was carried out using the paracoccidioidin delayed hypersensitivity test in 96 latin american wild mammals, including 49 arboreal animals (primates): 33 cebus apella (weeping-capuchin), 16 callithrix jacchus (marmoset); and 47 terrestrial animals (carnivora): 37 nasua nasua (coatimundi), and 10 felidae [panthera onca (jaguar), felis paradalis (ocel ...19957650577
evolution of alanine:glyoxylate aminotransferase 1 peroxisomal and mitochondrial targeting. a survey of its subcellular distribution in the livers of various representatives of the classes mammalia, aves and amphibia.as part of a wider study on the molecular evolution of alanine:glyoxylate aminotransferase 1 (agt1) intracellular compartmentalization, we have determined the subcellular distribution of immunoreactive agt1, using postembedding protein a-gold immunoelectron microscopy, in the livers of various members of the classes mammalia, aves, and amphibia. as far as organellar distribution is concerned, three categories could be distinguished. in members of the first category (type i), all, or nearly all, ...19947813517
phylogenetic reconstruction of south american felids defined by protein electrophoresis.phylogenetic associations among six closely related south american felid species were defined by changes in protein-encoding gene loci. we analyzed proteins isolated from skin fibroblasts using two-dimensional electrophoresis and allozymes extracted from blood cells. genotypes were determined for multiple individuals of ocelot, margay, tigrina, geoffroy's cat, kodkod, and pampas cat at 548 loci resolved by two-dimensional electrophoresis and 44 allozyme loci. phenograms were constructed using th ...19947932791
phylogenetic relationship between the iriomote cat and the leopard cat, felis bengalensis, based on the ribosomal dna.we analyzed the restriction fragment length polymorphisms in the spacer regions of ribosomal dna (rdna), using twelve restriction enzymes, to examine whether the iriomote cat is related to the leopard cat (felis bengalensis). a restriction map for each taxon was constructed and the major taxon-specific types of repeating unit (repetypes) were characterized on the basis of the arrangements of restriction sites. the iriomote cat and the leopard cat share a common repetype but this repetype is diff ...19947946460
epidemiological study of sporotrichosis and histoplasmosis in captive latin american wild mammals, são paulo, brazil.sporotrichosis and histoplasmosis are deep mycosis with a high incidence in human beings in brazil. in domestic animals histoplasmosis has been described only in dogs, but the occurrence of sporotrichosis among domestic animals in brazil has been described in dogs, cats, mules and asses. there is also a case of this disease reported in a chimpanzee (pan troglodites). the purpose of this research was to perform an epidomiological study of these mycoses using delayed hypersensitivity tests (histop ...19948028639
exchanges of short polymorphic dna segments predating speciation in feline major histocompatibility complex class i genes.sequence comparisons of 14 distinct mhc class i cdna clones isolated from species representing the three major taxonomic lineages of felidae (domestic cat lineage, ocelot lineage, and pantherine lineage) revealed that feline mhc class i alleles have highly mosaic structures with short polymorphic sequence motifs that are rearranged between alleles of individual mhc loci, between mhc class i genes within cat species, and between homologous mhc loci in different species. the pattern of sequence va ...19948064870
notoedric mange in an ocelot (felis pardalis) from southern texas.notoedric mange was diagnosed in a free-ranging adult male ocelot (felis pardalis) found dead in april 1994 in southern texas (usa). the emaciated carcass had no body fat. the heart was enlarged and flaccid. there was a nonpurulent serosanguineous pericardial and peritoneal exudate. severe encrusted skin lesions and alopecia extended from the head posteriorly to the shoulders. the forelegs and feet were less severely affected. we observed a massive infection of notoedres cati in skin scrapings. ...19958592392
responsiveness of ovaries to exogenous gonadotrophins and laparoscopic artificial insemination with frozen-thawed spermatozoa in ocelots (felis pardalis).adult female ocelots (felis pardalis) were treated with one of four dosages of equine chorionic gonadotrophin (ecg) and human chorionic gonadotrophin (hcg) (100 iu ecg/75 iu hcg, n = 3; 200 iu ecg/150 iu hcg, n = 4; 400 iu ecg/150 iu hcg, n = 5; 500 iu ecg/225 iu hcg, n = 5); hcg was administered 80 h after ecg. ovaries of each animal were evaluated by laparoscopy 39-43 h after hcg, and blood was collected for progesterone and oestradiol analysis. with progressive increases in gonadotrophin dosa ...19968667352
molecular phylogeny of mitochondrial cytochrome b and 12s rrna sequences in the felidae: ocelot and domestic cat lineages.molecular phylogeny of the cat family felidae is derived using two mitochondrial genes, cytochrome b and 12s rrna. phylogenetic methods of weighted maximum parsimony and minimum evolution estimated by neighbor-joining are employed to reconstruct topologies among 20 extant felid species. sequence analyses of 363 bp of cytochrome b and 376 bp of the 12s rrna genes yielded average pair-wise similarity values between felids ranging from 94 to 99% and from 85 to 99%, respectively. phylogenetic recons ...19968975691
phylogenetic reconstruction of the felidae using 16s rrna and nadh-5 mitochondrial genes.the felidae family represents a challenge for molecular phylogenetic reconstruction because it consists of 38 living species that evolved from a relatively recent common ancestor (10-15 million years ago). we have determined mitochondrial dna sequences from two genes that evolve at relatively rapid evolutionary rates, 16s rrna (379 bp) and nadh dehydrogenase subunit 5 (nadh-5, 318 bp), from multiple individuals of 35 species. based on separate and combined gene analyses using minimum evolution, ...19979071018
plasma cortisol levels in captive wild felines after chemical restraint.eight panthera onca (po), 13 felis concolor (fc), 7 felis yagouaroundi (fy), 7 felis tigrina (ft) and 5 felis pardalis (fp) specimens from são paulo state zoos were used. all animals were restrained with darts containing 10 mg/kg ketamine and 1 mg/kg xylazine. venous blood samples were collected as soon as possible (within 15-20 min) and serum was frozen until the time for cortisol quantification. cortisol was determined using a solid phase radioimmunoassay with an intra-assay coefficient of 8.5 ...19979532247
tracking the evolution of the elusive andean mountain cat (oreailurus jacobita) from mitochondrial dna.rarely observed in the wild, the existence of the andean mountain cat (oreailurus jacobita) has been established based on only 3 skulls and 14 museum skins. the andean mountain cat's evolutionary relationship to other felids based on morphological characters is largely contradictory, with evidence aligning it with south american small spotted cats (ocelot lineage) or alternatively with pantherine lineage felids. here we describe the phylogenetic distinctiveness and placement of the andean mounta ...19989656464
phylogeographic patterns and evolution of the mitochondrial dna control region in two neotropical cats (mammalia, felidae).the ocelot (leopardus pardalis) and margay (l. wiedii) are sister-species of neotropical cats which evolved from a lineage that migrated into south america during the formation of the panamanian land bridge 3-5 million years ago. patterns of population genetic divergence of each species were studied by phylogenetic analyses of mitochondrial dna (mtdna) control region sequences in individuals sampled across the distribution of these taxa. abundant genetic diversity and remarkably concordant phylo ...19989797412
disparate phylogeographic patterns of molecular genetic variation in four closely related south american small cat species.tissue specimens from four species of neotropical small cats (oncifelis geoffroyi, n = 38; o. guigna, n = 6; leopardus tigrinus, n = 32; lynchailurus colocolo, n = 22) collected from throughout their distribution were examined for patterns of dna sequence variation using three mitochondrial genes, 16s rrna, atp8, and nadh-5. patterns between and among o. guigna and o. geoffroyi individuals were assessed further from size variation at 20 microsatellite loci. phylogenetic analyses using mitochondr ...199910703553
novel gene conversion between x-y homologues located in the nonrecombining region of the y chromosome in felidae (mammalia).genes located on the mammalian y chromosome outside of the pseudoautosomal region do not recombine with those on the x and are predicted to either undergo selection for male function or gradually degenerate because of an accumulation of deleterious mutations. here, phylogenetic analyses of x-y homologues, zfx and zfy, among 26 felid species indicate two ancestral episodes of directed genetic exchange (ectopic gene conversion) from x to y: once during the evolution of pallas cat and once in a com ...200010805789
conservation value of a native forest fragment in a region of extensive agriculture.a survey of mammals and birds was carried out in a semi-deciduous forest fragment of 150 ha located in a zone of intensive agriculture in ribeirão preto, state of são paulo, south-eastern brazil. line transect sampling was used to census mammals and birds during six days, totalling 27.8 km of trails and 27.8 hours of observation. twenty mammal species were confirmed in the area (except bats and small mammals), including rare or endangered species, such as the mountain lion (puma concolor), the m ...200010959107
dietary separation of sympatric carnivores identified by molecular analysis of scats.we studied the diets of four sympatric carnivores in the flooding savannas of western venezuela by analysing predator dna and prey remains in faeces. dna was isolated and a portion of the cytochrome b gene of the mitochondrial genome amplified and sequenced from 20 of 34 scats. species were diagnosed by comparing the resulting sequences to reference sequences generated from the blood of puma (puma concolor), jaguar (panthera onca), ocelot (leopardus pardalus) and crab-eating fox (cerdocyon thous ...200011050553
twenty-four hour activity budgets and patterns of behavior in captive ocelots (leopardus pardalis).activity budgets of captive ocelots (leopardus pardalis) were assessed from over 547h of observational data obtained from six ocelots; two females at the dallas zoo (dallas, tx), two females at the caldwell zoo (tyler, tx) and a male and female at the fossil rim wildlife center (glen rose, tx). data were examined for the percentage of active behaviors exhibited during the day and nighttime hours; temporal patterns of active, pace, exploratory and marking behavior, and for significance in pacing ...200111179560
reproductive steroid hormones and ovarian activity in felids of the leopardus genus.reproductive endocrine patterns were characterized in female ocelots (leopardus pardalis; n = 3), tigrinas (leopardus tigrinus; n = 2), and margays (leopardus wiedii; n = 2) housed in captivity in southern brazil. females were maintained as singletons and exposed to natural fluctuations in photoperiod. cyclic changes in ovarian steroids were monitored by analyzing estrogen and progestogen metabolites in fecal samples collected five times weekly for 14 to 18 months. based on intervals between fec ...200111429781
sylvatic trichinellosis in texas.there are no published reports of domestic or sylvatic trichinellosis in texas. the aim of the present survey was to determine the presence of trichinella species in selected representative species of potential wildlife reservoirs in southern texas. in 1998-99, tongues of 211 wild mammals were collected in southern texas: 154 coyotes (canis latrans), three bobcats (lynx rufus), 32 racoons (procyon lotor), 13 opossum (didelphis marsupialis), four ocelots (leopardus pardalis) and five wild boars ( ...200111484391
sequence variation in the mitochondrial dna control region of wild african cheetahs (acinonyx jubatus).five hundred and twenty-five bp of mitochondrial control region were sequenced and analysed for 20 acinonyx jubatus and one felis catus. these sequences were compared with published sequences from another domestic cat, 20 ocelots (leopardus pardalus) and 11 margays (leopardus weidii). the intraspecific population divergence in cheetahs was found to be less than in the other cats. however variation was present and distinct groups of cheetahs were discernible. the 80 bp rs2 repetitive sequence mot ...200111488972
trichinella infection in wildlife of the southwestern united states.several potential mammalian reservoirs of sylvatic species of trichinella were examined from texas, new mexico, and arizona. during 1998-99, tongues were collected from a black bear (ursus americanus) in arizona; from 9 black bears, a coyote (canis latrans), and a mountain lion (felis concolor) in new mexico; and from 154 coyotes, 32 raccoons (procyon lotor), 13 opossums (didelphis marsupialis), 4 ocelots (leopardus pardalis), 3 bobcats (lynx rufus), and 5 feral hogs (sus scrofa) in southern tex ...200111695403
seroprevalence of toxoplasma gondii in captive neotropical felids from brazil.seroprevalence of toxoplasma gondii was determined in 865 captive neotropical felids from 20 states from brazil, sampled from september 1995 to april 1997. sera were tested by the modified agglutination test (mat) using formalin-fixed whole tachyzoites and mercaptoethanol. antibodies (mat> or =1:20) to t. gondii were found in 472 of 865 (54.6%) cats: in 45 of 99 (45.9%) jaguarundis (herpailurus yagouaroundi), in 97 of 168 (57.7%) ocelots (leopardus pardalis), in 68 of 131 (51.9%) oncillas (l. ti ...200111777601
seasonal analysis of semen characteristics, serum testosterone and fecal androgens in the ocelot (leopardus pardalis), margay (l. wiedii) and tigrina (l. tigrinus).captive adult male ocelots (leopardus pardalis, n = 3), margays (l. wiedii, n = 3) and tigrinas (l. tigrinus, n = 4) in two locations in southern brazil were studied for 14 consecutive months to evaluate the effect of season on testicular function. reproductive evaluations, including testicular measurements, electroejaculation and blood collection were conducted monthly. fecal samples were collected weekly for androgen metabolite analysis to assess testicular steroidogenic activity. ocelots had ...200212066863
serum concentrations of lipids, vitamin d metabolites, retinol, retinyl esters, tocopherols and selected carotenoids in twelve captive wild felid species at four zoos.serum concentrations of several nutrients were measured in 12 captive wild felid species including caracal (felis caracal), cheetah (acinonyx jubatus), cougar (felis concolor), fishing cat (felis viverrinus), leopard (panthera pardus), lion (panthera leo), ocelot (felis pardalis), pallas cat (felis manul), sand cat (felis margarita), serval (felis serval), snow leopard (panthera uncia) and tiger (panthera tigris). diet information was collected for these animals from each participating zoo (broo ...200312514284
serosurvey for feline leukemia virus and lentiviruses in captive small neotropic felids in são paulo state, brazil.feline leukemia virus (felv), gammaretrovirus, and feline immunodeficiency virus, a lentivirus, are members of the family retroviridae, and may establish persistent infections in the domestic cat (felis catus). cytoproliferative and cytosuppressive disorders may result from infection with these viruses. morbidity and mortality rates are high in domestic cats worldwide. infection of endangered neotropic small felids with these viruses could be devastating. to investigate the prevalence of felv an ...200312723802
helminths of the ocelot from southern texas.in the usa, the ocelot (leopardus pardalis) is a highly endangered felid found only in a few remaining vestiges of native thornshrub brushland in the lower rio grande valley (lrgv) of extreme southern texas. from 1987-1998, carcasses of 15 adult ocelots that died of vehicular accidents or natural causes were examined for helminths. all cats had 1-8 (mean = 3) helminth species. all were infected with 1-101 (mean +/- se = 32 +/- 7) toxascaris leonina. other helminths from these ocelots were alaria ...200314567231
international training programs in reproductive sciences for conservation of latin american felids.survival of the ten non-domestic felid species endemic to latin america is imperiled by habitat loss, poaching and poor captive management. over the past 10 years, conservation of these felids has been the primary focus of a reproductive research and training program conducted in brazil, mexico, and the usa. the objectives of this program were to: (1) provide intensive training in reproductive sciences to latin american scientists, (2) conduct collaborative studies investigating basic and applie ...200415271441
seroprevalence and genomic divergence of circulating strains of feline immunodeficiency virus among felidae and hyaenidae species.feline immunodeficiency virus (fiv) infects numerous wild and domestic feline species and is closely related to human immunodeficiency virus (hiv) and simian immunodeficiency virus (siv). species-specific strains of fiv have been described for domestic cat (felis catus), puma (puma concolor), lion (panthera leo), leopard (panthera pardus), and pallas' cat (otocolobus manul). here, we employ a three-antigen western blot screening (domestic cat, puma, and lion fiv antigens) and pcr analysis to sur ...200515956574
ticks (acari: ixodida) on wild carnivores in brazil.the present study reports field data of ticks infesting wild carnivores captured from july 1998 to september 2004 in brazil. additional data were obtained from one tick collection and from previous published data of ticks on carnivores in brazil. during field work, a total of 3437 ticks were collected from 89 cerdocyon thous (crab-eating fox), 58 chrysocyon brachyurus (maned wolf), 30 puma concolor (puma), 26 panthera onca (jaguar), 12 procyon cancrivorus (crab-eating raccoon), 4 speothos venati ...200516082932
gastroinvasive helicobacter infection in an ocelot (leopardus pardalis).highly invasive helicobacter-like organisms were found in a 19-year-old female ocelot (leopardus pardalis) with multiple ulcers in the fundic region of the stomach. the bacteria, resembling helicobacter heilmannii, were located largely within canaliculi or in the cytosol of parietal cells. except in the ulcerative lesions, parietal cells were hyperplastic, while chief cells and neck mucous cells were reduced in number. the term "gastroinvasive helicobacter-like organism" was applied. it seems pr ...200516154138
ovarian response to repeated administration of alternating exogenous gonadotropin regimens in the ocelot (leopardus pardalis) and tigrinus (leopardus tigrinus).exogenous gonadotropins are used to stimulate ovarian follicular growth and ovulation in mammalian species, including wild cats. however, successes in application of assisted reproduction techniques in nondomestic felids have been sparse. our objectives were to assess the effectiveness of alternating gonadotropin regimens on ovarian responses. five adult female ocelots and four adult female tigrinus were treated four to six times, using alternating ecg/hcg and pfsh/plh at 4-month intervals. lapa ...200616472853
application of assisted reproduction for population management in felids: the potential and reality for conservation of small cats.assisted reproductive technology (art), using the primary applied tools of ai, et, and sperm and embryo cryopreservation, has been promoted over the past decades for its potential to conserve endangered wildlife, including felids. however, if the goal is efficient, consistent production of viable offspring for population management, then the 'potential' of art has yet to become 'reality' for any non-domestic cat species. for the five small-sized felids (i.e., brazilian ocelot, fishing cat, palla ...200616650889
first evidence of feline herpesvirus, calicivirus, parvovirus, and ehrlichia exposure in brazilian free-ranging felids.serum samples from 18 pumas (puma concolor), one ocelot (leopardus pardalis), and two little spotted cats (leopardus tigrinus) collected from free-ranging animals in brazil between 1998 and 2004 were tested by indirect immunofluorescence (ifa) for antibodies to feline herpesvirus 1 (fhv 1), calicivirus (fcv), coronavirus (fcov), parvo-virus (fpv), ehrlichia canis, anaplasma pha-gocytophilum, and bartonella henselae. serum samples also were tested, by western blot and elisa, for feline leukemia v ...200616870878
risk factors associated with sero-positivity to toxoplasma gondii in captive neotropical felids from brazil.from september 1995 to february 2001, blood samples were collected from 865 neotropical felids belonging to 8 different species. these animals were housed in 86 institutions located in 78 cities of 20 brazilian states. our goal was to identify the risk factors associated with sero-positivity to toxoplasma gondii in captive neotropical felids from brazil. all serum samples were tested by the modified agglutination test (mat), using formalin-fixed whole tachyzoites and mercaptoethanol. for each an ...200717140683
interspecific scaling of the morphology and posture of the limbs during the locomotion of cats (felidae).for phylogenetically diverse mammals, ranging from small rodents to large ungulates, the generalization that limb erectness increases with increased size is supported by some size-dependent scaling relationships of appendicular skeletal anatomy as well as a limited number of direct observations of limb posture during locomotion. if size alone is the causal basis for different limb posture, then the erectness of limbs should increase significantly with increased size within a phylogenetically nar ...200717267650
worldwide occurrence of feline hemoplasma infections in wild felid species.while hemoplasma infections in domestic cats are well studied, almost no information is available on their occurrence in wild felids. the aims of the present study were to investigate wild felid species as possible reservoirs of feline hemoplasmas and the molecular characterization of the hemoplasma isolates. blood samples from the following 257 wild felids were analyzed: 35 iberian lynxes from spain, 36 eurasian lynxes from switzerland, 31 european wildcats from france, 45 lions from tanzania, ...200717301277
neutralizing antibodies against feline herpesvirus type 1 in captive wild felids of brazil.feline herpesvirus type 1 infection affects domestic cats, causing mainly upper respiratory tract diseases. although this infection has been described in captive and free-ranging wild felids from europe, asia, north america, and africa, no information is available on its occurrence among wild felids of brazil. in this study, 250 serum samples of six species of brazilian captive wild felids (leopardus tigrinus, leopardus wiedii, herpailurus yaguarondi, puma concolor, leopardus pardalis, and panth ...200517312763
parasites of free-ranging small canids and felids in the bolivian chaco.parasite surveys of free-ranging wildlife provide important information for monitoring population health. between march 2001 and march 2003, we sampled 10 ocelots (leopardus pardalis), eight geoffroy's cats (oncifelis geoffroyi), a jaguarundi (herpailurus yaguarondi), five pampas foxes (pseudalopex gymnocercus), and three crab-eating foxes (cerdocyon thous) at three sites in the bolivian chaco. the objective of the study was to survey the parasite fauna of these carnivores and compare prevalence ...200617312790
disseminated coccidioidomycosis in a captive indochinese tiger (panthera tigris corbetti) with chronic renal disease.a 19-yr-old, 78.2-kg captive female indochinese tiger (panthera tigris corbetti) from the el paso zoo (el paso, texas, usa) with chronic renal disease was euthanized after a 10-day course of anorexia, depression, progressive rear limb weakness, muscle fasciculations, and head tremors. postmortem findings included pericardial effusion, generalized lymphadenopathy, glomerulosclerosis, glomerular atrophy with membranous glomerulonephropathy, and pancreatic adenocarcinoma. pyogranulomatous pneumonia ...200617315442
ocelot (leopardus pardalis) predation on primates in caratinga biological station, southeast brazil.this study demonstrates that ocelots (leopardus pardalis) extensively use primates as a food resource at the caratinga biological station (cbs) in southeast brazil. analysis of 60 fecal samples collected over 4 years revealed predation upon the brown howler monkey (alouatta guariba), the muriqui (brachyteles hypoxanthus), and the brown capuchin monkey (cebus apella). the most frequent items found in the fecal samples analyzed were calomys (n=16) and non-identified aves (n=15), followed by a. gua ...200717330310
measurement of thyroid hormones (thyroxine, t4; triiodothyronine, t3) in captive nondomestic felids.the aim of this research was to obtain basic values for the evaluation of thyroid function in nondomestic felids. serum thyroid hormone concentrations (thyroxine, t4; triiodothyronine, t3) were measured by radioimmunoassay in 145 cats, representing nine species of captive nondomestic felids: jaguar (panthera onca), n = 49; puma (puma concolor), n = 10; ocelot (leopardus pardalis), n = 22; oncilla (leopardus tigrinus), n = 12; geoffroy (oncifelis geoffroyi), n = 4; jaguarundi (herpailurus yaguaro ...200717469287
comparison of different sperm cryopreservation procedures on post-thaw quality and heterologous in vitro fertilisation success in the ocelot (leopardus pardalis).cryopreservation of spermatozoa from free-living ocelots (leopardus pardalis) could benefit their conservation by facilitating gene flow between in situ and ex situ populations without requiring removal of additional cats from the wild. the objective of this study was to investigate three different methods of ocelot sperm cryopreservation to identify the most appropriate technique for use in a field environment. male ocelots (n = 10), housed in north american zoos, were anaesthetised with tileta ...200717601417
genetic diversity of microsatellite loci in leopardus pardalis, leopardus wiedii and leopardus tigrinus.the microsatellite loci fca045, fca077, fca008, and fca096 are highly variable molecular markers which were used to determine the genetic diversity in 148 captive leopardus sp. the pcr-amplified products of microsatellite loci were characterized in abi prism 310 genetic analyzer. allele numbers, heterozygosity, polymorphism information content, exclusive allele number, and shared alleles were calculated. sixty-five alleles were found and their sizes ranged from 116 to 216 bp in four microsatelli ...200717624861
sperm cryopreservation in endangered felids: developing linkage of in situ-ex situ populations.many of the world's cat species face growing threats to their continued survival in nature. for some species, managed captive populations may provide a reservoir for future reintroduction or genetic augmentation. because most zoo populations are derived from small founder sizes and are subject to loss of genetic variation over time, periodic infusion of founder alleles is necessary to avoid the dire consequences of inbreeding. collection and freezing of semen from free-living nondomestic felids ...200717644981
serosurvey of small carnivores in the bolivian chaco.five species of bolivian carnivores, including nine geoffroy's cats (oncifelis geoffroyi), ten ocelots (leopardus pardalis), one jaguarundi (herpailurus yaguarondi), nine pampas foxes (pseudalopex gymnocercus), and five crab-eating foxes (cerdocyon thous) were sampled between march 2001 and april 2005 and tested for antibodies to common pathogens of domestic carnivores. carnivores were trapped in three areas: a village, the region between human settlements and a protected area, and within kaa-iy ...200717699100
influence of cooling rate on the ability of frozen-thawed sperm to bind to heterologous zona pellucida, as assessed by competitive in vitro binding assays in the ocelot (leopardus pardalis) and tigrina (leopardus tigrinus).we evaluated the influence of two cooling rates (from 25 to 5 degrees c) on post-thaw function of frozen sperm in ocelots (leopardus pardalis; n=3 males) and tigrinas (leopardus tigrinus; n=4 males). seven normospermic (>70% normal sperm) electroejaculates from each species were diluted with a 4% glycerol freezing medium, divided into two aliquots, and assigned to one of two cooling rates: fast or slow (0.7 or 0.16 degrees c/min, respectively). sperm motility index (smi) and percentage of sperm ...200817977588
variability in assays used for detection of lentiviral infection in bobcats (lynx rufus), pumas (puma concolor), and ocelots (leopardus pardalis).although lentiviruses similar to feline immunodeficiency virus (fiv) are known to infect numerous felid species, the relative utility of assays used for detecting lentiviral infection has not been compared for many of these hosts. we tested bobcats (lynx rufus), pumas (felis concolor), and ocelots (leopardus pardalis) for exposure to lentivirus using five different assays: puma lentivirus (plv), african lion lentivirus (llv), and domestic cat fiv-based immunoblots, a commercially available enzym ...200717984266
the first report of hepatozoon sp. (apicomplexa: hepatozoidae) in neotropical felids from brazil.in order to investigate the occurrence of hepatozoon infection in neotropical felids from brazil, blood from the jugular or cephalic vein was taken from 29 non-domestic felids including ocelot (leopardus pardalis), little spotted cat (leopardus tigrinus), margay (leopardus wiedii), and jaguarondi (puma yagouaroundi) from the northeast region of brazil. hepatozoon infection was confirmed by light microscopy and molecular techniques. the results showed five naturally infected felids. partial seque ...200818243562
[seasonal evaluation of mammal species richness and abundance in the "mário viana" municipal reserve, mato grosso, brasil].we evaluated seasonal species presence and richness, and abundance of medium and large sized mammalian terrestrial fauna in the "mário viana" municipal biological reserve, nova xavantina, mato grosso, brazil. during 2001, two monthly visits were made to an established transect, 2,820 m in length. records of 22 mammal species were obtained and individual footprint sequences quantified for seasonal calculation of species richness and relative abundance index (x footprints/km traveled). all 22 spec ...200618491629
ocelots on barro colorado island are infected with feline immunodeficiency virus but not other common feline and canine viruses.transmission of pathogens from domestic animals to wildlife populations (spill-over) has precipitated local wildlife extinctions in multiple geographic locations. identifying such events before they cause population declines requires differentiating spillover from endemic disease, a challenge complicated by a lack of baseline data from wildlife populations that are isolated from domestic animals. we tested sera collected from 12 ocelots (leopardus pardalis) native to barro colorado island, panam ...200818689668
domestic dogs in atlantic forest preserves of south-eastern brazil: a camera-trapping study on patterns of entrance and site occupancy rates.presence of exotic species in forest remnants is a major concern for the conservation of wild species, not only on islands, where potential impact is higher. although the problem is widespread and increasing, there are few studies on neotropical forests. here we quantify the occurrence of domestic dogs (canis lupus familiaris) in an atlantic forest reserve in south-eastern brazil (santa lúcia biological station--slbs). throughout two years of monitoring with camera traps (2,142 camera-days), 25 ...200819197494
molecular detection of cytauxzoon spp. in asymptomatic brazilian wild captive felids.cytauxzoon spp. dna was detected for the first time in blood samples from asymptomatic brazilian wild captive felids. in 2006, 72 edta blood samples from seven wild felids species: puma concolor (puma), leopardus pardalis (ocelot), puma yagouaroundi (jaguarundi), leopardus wiedii (margay), leopardus tigrinus (little spotted cat), oncifelis colocolo (pampas cat) and panthera onca (jaguar) were analyzed using polymerase chain reaction to amplify the 18s rrna gene segment in order to verify the pre ...200919204356
first evidence of canine distemper in brazilian free-ranging felids.serum samples from 19 jaguars (panthera onca), nine pumas (puma concolor), and two ocelots (leopardus pardalis) were collected between january 1999 and march of 2005 and tested for presence of canine distemper virus (cdv). all cats were free-ranging animals living in two protected areas in the brazilian atlantic forest. in addition, 111 domestic dogs from nearby areas were sampled for cdv. our results show the first evidence of cdv exposure in brazilian free-ranging felids. from the 30 samples a ...200919259737
comparison of several types of enrichment for captive felids.enrichment can increase the complexity of the captive environment and possibly enhance captive animals' well-being by stimulating active behaviors and reducing stereotypical behaviors commonly seen in zoo felids. in this study, three different enrichment items were added to outdoor enclosures of felids at the montgomery zoo to test their effects on activity levels and stereotypic pacing. bones, frozen fish, and spices (cinnamon, chili powder, and cumin) were presented over a 3-month period to si ...200719360587
survey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from southern brazil.a total of 57 captive neotropical felids (one leopardus geoffroyi, 14 leopardus pardalis, 17 leopardus wiedii, 22 leopardus tigrinus, and three puma yagouaroundi) from the itaipu binacional wildlife research center (refúgio bela vista, southern brazil) were anesthetized for blood collection. feces samples were available for 44 animals, including one l. geoffroyi, eight l. pardalis, 14 l. wiedii, 20 l. tigrinus, and one p. yagouaroundi. total dna and rna were extracted from blood and feces, respe ...200919569487
leishmania(leishmania) chagasi in captive wild felids in brazil.this study used a pcr-rflp test to determine the presence of leishmania (leishmania) chagasi in 16 captive wild felids [seven puma concolor (linnaeus, 1771); five panthera onca (linnaeus, 1758) and four leopardus pardalis (linnaeus, 1758)] at the zoological park of the federal university of mato grosso, brazil. amplification of leishmania spp. dna was seen in samples from five pumas and one jaguar, and the species was characterized as l. chagasi using restriction enzymes. it is already known tha ...201019740501
testis stereology, seminiferous epithelium cycle length, and daily sperm production in the ocelot (leopardus pardalis).similar to most wild felids, the ocelot (leopardus pardalis) is an endangered species. however, knowledge regarding reproductive biology of the ocelot is very limited. germ cell transplantation is an effective technique for investigating spermatogenesis and stem cell biology in mammals, and the morphologic characterization of germ cells and knowledge of cycle length are potential tools for tracking the development of transplanted germ cells. our goal was to investigate basic aspects related to t ...201019853903
detection of bartonella spp. in neotropical felids and evaluation of risk factors and hematological abnormalities associated with infection.although antibodies to bartonella henselae have been described in all neotropical felid species, dna has been detected in only one species, leopardus wiedii. the aim of this study was to determine whether dna of bartonella spp. could be detected in blood of other captive neotropical felids and evaluate risk factors and hematological findings associated with infection. blood samples were collected from 57 small felids, including 1 leopardus geoffroyi, 17 l. wiedii, 22 leopardus tigrinus, 14 leopa ...201019913372
nutritional composition and digestibility by ocelots (leopardus pardalis) of whole animals and a commercial diet.felids are adapted to eat whole prey, but in north american zoos are usually fed processed diets based on muscle meat. we analyzed proximate nutrient composition and digestibility by ocelots of a commercial processed diet and whole animals of five species. the processed diet did not differ significantly from the whole animals in proximate composition, although it was at one end of the range of results for all nutrients. domestic chicks were significantly lower than all other dietary items tested ...201020024961
stress in wildlife species: noninvasive monitoring of glucocorticoids.depression and stress are related pathologies extensively studied in humans. however, this relationship is not well known in animals kept in zoos and even less known in wild animals. in zoo animals, acute and chronic stress caused by difficulties in coping with stressors such as public presence and noise, among others, can induce the appearance of repetitive pathological behaviors such as stereotypies, many times associated with organic pathologies that deeply affect their health and welfare. in ...201020134205
serological survey of toxoplasma gondii in captive neotropical felids from southern brazil.toxoplasma gondii is the causative intracellular protozoan of toxoplasmosis in human being and animals. members of the felidae family are considered the single definitive host for the infection; both wild and domestic cats are able to excrete oocysts in the environment. wild cats maintained in captivity may serve as source of infection for other clinically susceptible animals in the same environment. the aim of this study was to determine the frequency of t. gondii igg antibodies in 57 neotropic ...201020472340
renal lesions of nondomestic felids.to comprehensively evaluate the occurrence of renal lesions in a variety of nondomestic felids, necropsy cases from 1978 to 2008 were reviewed from a municipal zoo and a large cat sanctuary for those in which the kidneys were examined histologically. seventy exotic felids were identified (25 tigers, 18 lions, 6 cougars, 5 leopards, 3 snow leopards, 3 clouded leopards, 3 canadian lynx, 2 ocelots, 2 bobcats, 2 cheetahs, 1 jaguar), and their histologic renal lesions were evaluated and compared. the ...201020876911
female reproductive cycles of wild female felids.many felid species are endangered because of destructive human activities. as a result, zoos are being tasked with sustaining genetically healthy populations in case of catastrophic extinctions. unfortunately, with the exception of a few species, most felids do not reproduce well in captivity. the ability to track reproductive activity via hormones is key to developing successful ex situ breeding programs. through the development of noninvasive fecal hormone monitoring techniques, a high degree ...201020888156
dna extraction from hair shafts of wild brazilian felids and canids.wild felids and canids are usually the main predators in the food chains where they dwell and are almost invisible to behavior and ecology researchers. due to their grooming behavior, they tend to swallow shed hair, which shows up in the feces. dna found in hair shafts can be used in molecular studies that can unravel, for instance, genetic variability, reproductive mode and family structure, and in some species, it is even possible to estimate migration and dispersion rates in given populations ...201021174262
identification of neotropical felid faeces using rcp-pcr.faeces similarity among sympatric felid species has generally hampered their use in distributional, demographic and dietary studies. here, we present a new and simple approach based on a set of species-specific primers, for the unambiguous identification of faeces from sympatric neotropical felids (i.e. puma, jaguar, jaguarundi and ocelot/ margay). this method, referred to as rapid classificatory protocol-pcr (rcp-pcr), consists of a single-tube multiplex pcr yielding species-specific banding pa ...201121429118
serological detection of viral infections in captive wild cats from costa rica.serum samples from a total of 44 wildcats, 28 margays (leopardus wiedii), 10 ocelots (leopardus pardalis), four jaguaroundis (herpailurus yaguaroundi), one oncilla (leopardus tigrina), and one jaguar (panthera onca) were obtained between january 2001 and august 2002 from the profelis centre for rehabilitation of wild felids, located in the northwestern region of costa rica. forty three samples were tested for antibodies against feline immunodeficiency virus (fiv) and p27 antigen of feline leukem ...201121547230
germ cell transplantation in felids: a potential approach to preserving endangered species.with the exception of the domestic cat, all members from the family felidae are considered either endangered or threatened. although not yet used with this purpose, spermatogonial stem cell (ssc) transplantation has a high potential to preserve the genetic stock of endangered species. however, this technique has not previously been established in felids. therefore, we developed the necessary procedures to perform syngeneic and xenogeneic ssc transplants [e.g., germ cell (gc) depletion in the rec ...201121597091
food web connections and the transmission cycles of trypanosoma cruzi and trypanosoma evansi (kinetoplastida, trypanosomatidae) in the pantanal region, brazil.we examined by parasitological tests (hemocultures and buffy coat) infection by trypanosoma cruzi and t. evansi in blood samples from leopardus pardalis, cerdocyon thous and domestic dogs. besides, 25 t. cruzi isolates previously derived from feral pigs and small wild mammals were here characterized by miniexon gene and demonstrated to be in the tci genotype. herein, we make an overall analysis of the transmission cycle of both trypanosome species in the light of the assemblage of data collected ...201121600622
First record of notoedric mange in ocelot (Leopardus pardalis Linnaeus, 1758) in the amazon region, Brazil.This paper describes a case of notoedric mange in an ocelot (Leopardus pardalis) in the Brazilian Amazon region. A young male of approximately four months of age that was illegally kept as a pet was apprehended in Altamira, State of Pará, northern Brazil. The animal was transported to the Mangal das Garças Park in the state's capital city of Belém. The ocelot had pruritus and lesions suggestive of scabies in the head. Skin scraping material was examined under optic microscopy. There was seen a l ...201122166390
using pgfm (13,14-dihydro-15-keto-prostaglandin f2α) as a non-invasive pregnancy marker for felids.understanding the complex endocrine interactions that control reproduction in felids is essential for captive breeding management. the most important demand is a quick and reliable pregnancy diagnosis. however, the occurrence of pseudopregnancies in felids complicates matters. we investigated whether the fecal prostaglandin metabolite (pgfm) recently suggested for pregnancy diagnosis in the lynx is suitable for all felid species. we found that increased levels of pgfm during the last trimester i ...201122192399
hemoplasmas in wild canids and felids in brazil.hemotropic mycoplasmas, epicellular erythrocytic bacterial parasites lacking a cell wall, are the causative agents of infectious anemia in numerous mammalian species. the presence of hemotropic mycoplasmas in blood samples of neotropical and exotic wild canids and felids from brazilian zoos were recorded using molecular techniques. blood samples were collected from 146 brazilian wild felids, 19 exotic felids, 3 european wolves (canis lupus), and from 97 brazilian wild canids from zoos in the bra ...201122946419
high proportion of male faeces in jaguar populations.faeces provide relevant biological information which includes, with the application of genetic techniques, the sex and identity of individuals that defecated, thus providing potentially useful data on the behaviour and ecology of individuals, as well as the dynamics and structure of populations. this paper presents estimates of the sex ratio of different felid species (jaguar, panthera onca; puma, puma concolor; and ocelot/margay, leopardus pardalis/leopardus wiedi) as observed in field collecte ...201223285226
cross transmission of gastrointestinal nematodes between captive neotropical felids and feral cats.pathogen cross transmission between wildlife and domestic animals represents an extinction risk for wildlife; however, reliable verification is difficult to perform, and in some cases, it is even considered unlikely to be conducted. the aim of this work was to identify cross transmission of helminths between feral cats and captive wild felids at a zoological park in southeastern mexico. feces were collected from jaguars (panthera onca), cougars (puma concolor), ocelots (leopardus pardalis), marg ...201324450052
molecular and serological detection of babesia spp. in neotropical and exotic carnivores in brazilian zoos.large and small piroplasms have been observed in the blood smears of various wild carnivores, but few studies utilizing molecular characterization have been done. the goal of this present study was to investigate the presence of babesia sp. by molecular and serologic techniques in exotic and neotropical carnivores maintained in captivity at brazilian zoos. blood and sera samples were collected from 146 brazilian wild felids, 21 exotic felids, 1 genet (genetta tigrina), 3 european wolves (canis l ...201122946386
exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus and parvovirus in the cerrado of central brazil.human population growth around protected areas increases the contact between wild and domestic animals, promoting disease transmission between them. this study investigates the exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus (cdv) and parvovirus in emas national park (enp) in the cerrado savanna of central brazil. serum samples were collected from 169 wild carnivores, including the maned wolf (chrysocyon brachyurus), crab-eating fox (cerdocyon thous), hoary f ...201627469023
canine distemper virus in wild felids of costa rica.several highly infectious diseases can be transmitted through feces and cause elevated mortality among carnivore species. one such infectious agent, canine distemper virus (cdv; paramyxoviridae: morbillivirus), has been reported to affect wild carnivores, among them several felid species. we screened free-ranging and captive wild carnivores in costa rica for cdv. between 2006 and 2012, we collected 306 fecal samples from 70 jaguars (panther onca), 71 ocelots ( leopardus pardalis ), five jaguarun ...201626967127
clostridium difficile and clostridium perfringens from wild carnivore species in brazil.despite some case reports, the importance of clostridium perfringens and clostridium difficile for wild carnivores remains unclear. thus, the objective of this study was to identify c. perfringens and c. difficile strains in stool samples from wild carnivore species in brazil. a total of 34 stool samples were collected and subjected to c. perfringens and c. difficile isolation. suggestive colonies of c. perfringens were then analyzed for genes encoding the major c. perfringens toxins (alpha, bet ...201424979683
clostridium difficile-associated diarrhea in an ocelot (leopardus pardalis).the aim of this study is to report a case of clostridium difficile-associated diarrhea in an ocelot (leopardus pardalis) in the state of mato grosso do sul, brazil. the animal, a 24-month-old male, was referred to the centro de reabilitação de animais silvestres (cras) with a history of having been run over and tibia and fibula fractures. after a surgery to repair the fractures, the ocelot underwent antibiotic therapy with two doses of sodium cefovecin, during which he presented with diarrhea. a ...201323467074
feline panleukopaenia virus in captive non-domestic felids in south africa.an outbreak of feline panleukopaenia virus (fplv) infection was diagnosed by pathology, electron microscopy and polymerase chain reaction (pcr) in vaccinated captive-bred subadult cheetahs in south africa. subsequent to this disease outbreak, 12 cases of fplv diagnosed on histology were confirmed by pcr in captive african black-footed cat, caracal, cheetah, lion, ocelot and serval. phylogenetic analyses of the viral capsid protein gene on pcr-positive samples, vaccine and national center for bio ...201627380652
occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a brazilian wetland.hemotropic mycoplasmas are known to cause anemia in several mammalian species. the present work aimed to investigate the occurrence of mycoplasma spp. in wild mammals, domestic dogs and their respective ectoparasites, in southern pantanal region, central-western brazil. between august 2013 and march 2015, 31 nasua nasua, 78 cerdocyon thous, seven leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials were trapped and ectoparasites (ticks and fleas) found parasitizing the animals were c ...201728366511
molecular and morphologic characterization of sarcocystis felis (apicomplexa: sarcocystidae) in south american wild felids from brazil.wild felids are thought to share parasites with domestic cats. however, little is known of the coccidian parasites of wild felids. we investigated the presence of sarcocystis spp. in tissues of 6 species of 90 neotropical small felids killed in road accidents in the state of rio grande do sul, brazil by using microscopic and molecular techniques. formalin-fixed tissues from 28 felids were examined, and sarcocystis felis-like sarcocysts were detected in 4 wild cats (2 puma yagouaroundi and 2 leop ...201626827854
molecular identification of spirometra spp. (cestoda: diphyllobothriidae) in some wild animals from brazil.species of the genus spirometra are diphyllobothriid tapeworms with complex life cycles and are involved in human sparganosis, a neglected disease that affects individuals worldwide. although some species were reported in wild felids and human cases of sparganosis were described in brazil, the biology and taxonomy of these parasites are poorly understood. in the present study, samples of diphyllobothriids (eggs and/or proglottids) obtained from the stools of wild carnivores (leopardus pardalis a ...201627235572
toxoplasmosis seroprevalence in wild small rodents, potentially preys of ocelots in north-eastern mexico.the aim of this study was to assess the prevalence of toxoplasma gondii infection in rodents that coexist with ocelots in north-eastern mexico. eighty rodents of five genera were captured and their serum samples tested for specific igg antibodies to t. gondii by in-house indirect elisa using three different conjugates. prevalences of 7% (3/44) and 33% (4/12) were found in sigmodon hispidus and liomys irroratus, respectively, and were significantly different. all baiomys taylori and oligoryzomys ...201425375977
seroprevalence of toxoplasma gondii infection in captive mammals in three zoos in mexico city, mexico.antibodies to toxoplasma gondii were determined in 167 mammals in three zoos in mexico city, mexico, using the modified agglutination test (mat). overall, antibodies to t. gondii were found in 89 (53.3%) of the 167 animals tested. antibodies were found in 35 of 43 wild felidae: 2 of 2 bobcats (lynx rufus); 4 of 4 cougars (puma concolor); 10 of 13 jaguars (panthera onca); 5 of 5 leopards (panthera pardus); 7 of 7 lions (panthera leo); 2 of 3 tigers (panthera tigris); 2 of 3 ocelots (leopardus par ...201324063119
toxoplasma gondii in free-ranging wild small felids from brazil: molecular detection and genotypic characterization.brazil harbors the largest number of wild neotropical felid species, with ten of the twelve species recorded in the american continent. although these animals are considered to be definitive hosts for toxoplasma gondii, there are few descriptions of the parasite in these species. here, we performed a molecular detection of t. gondii by amplification of the marker its-1 from tissue samples obtained from 90 free-ranging wild small neotropical felids from rio grande do sul - brazil. of the sampled ...201323932730
prevalence of anti-toxoplasma gondii antibody in free-ranging ocelots (leopardus pardalis) from tamaulipas, mexico. 201222740559
the multiple and complex and changeable scenarios of the trypanosoma cruzi transmission cycle in the sylvatic environment.in this study, we report and discuss the results generated from over 20 years of studies of the trypanosoma cruzi sylvatic transmission cycle. our results have uncovered new aspects and reviewed old concepts on issues including reservoirs, true generalist species, association of mammalian species with distinct discrete typing units - dtus, distribution of t. cruzi genotypes in the wild, mixed infections, and t. cruzi transmission ecology. using parasitological and serological tests, we examined ...201526200785
trypanosoma cruzi tci and tcii transmission among wild carnivores, small mammals and dogs in a conservation unit and surrounding areas, brazil.aiming to better understand the ecological aspects of trypanosoma cruzi transmission cycles, wild carnivores, small mammals and dogs were examined for t. cruzi infection in the serra da canastra national park region, brazil. isolates were genotyped using mini-exon gene and pcr-rflp (1f8 and h3) genomic targets. trypanosoma cruzi transmission was well established in the area and occurred in both wild and peridomestic environments. dog seroprevalence was 29·4% (63/214) and tci and tcii genotypes, ...201323062278
high consumption of primates by pumas and ocelots in a remnant of the brazilian atlantic forest.we studied the diet of the ocelot and puma during the years 2007 and 2008 at the feliciano miguel abdala reserve, in minas gerais, south-eastern brazil. we collected 49 faecal samples (scats) from cats, and identified the species of cat from 23 of them by the analysis of the microstructure patterns of hairs found in their faeces: 17 scats of the puma (puma concolor) and six of the ocelot (leopardus pardalis). in the puma scats, we identified three species of primates (brachyteles hypoxanthus, al ...201425296212
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