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antibodies reactive with leptotrichia buccalis in human serum from infancy to adulthood. 19768029
blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects.cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of actinomyces viscosus (av), actinomyces naeslundii (an), veillonella alcalescens (va), leptotrichia buccalis (lb), b ...197610259
serologic reactions of oral gram negative anaerobic bacilli.serological reactions were performed using hyperimmune rabbit antisera and antigenic preparations of leptotrichia buccalis, fusobacterium fusiforme, and fusobacterium polymorphum. all tests indicated that there was serologic cross reactivity between the two fusobacterium species. no cross reactivity could be detected between the fusobacterium species and l. buccalis. the findings suggest that f. fusiform and f. polymorphum are similar in their immunogenicity, and that the grouping of these two o ...1979105024
antigens of leptotrichia buccalis i. their serologic reaction with human sera. 1975127031
antigens of leptotrichia buccalis ii. their reaction with complement fixing igm in human sera. 1975127032
leptotrichia buccalis hemagglutination in cell binding and salivary inhibition studies.the characteristic hemagglutination (ha) of leptotrichia buccalis was used for measuring its attachment to various human cells and for determining if saliva contained hemagglutination inhibition (hi) factors. the microbial strain utilized displayed the characteristic em morphology of l. buccalis. sonicated preparations of the organism were tested for ha activity before and after adsorption with human cells. buccal epithelial cells, red blood cells (rbc), hela and embryonic kidney cells all bound ...1978151130
properties of the human salivary aggregating factor for leptotrichia buccalis cells. 1978280284
oral fusobacterium, leptotrichia and bacterionema: i. hisorical survey and taxonomic considerations. 1977370082
oral fusobacterium, leptotrichia and bacterionema: ii. pathogenicity: a review of the literature. 1977370083
acute appendicitis and bacteroides fragilis.bacteria belonging to the bacteroides fragilis group (b. fragilis, b. ovatus, b. vulgatus, b. distasonis, b. thetaiotaomicron and b. uniformis) were cultured in quantities of less than or equal to 10(5) c.f.u./ml from the excised appendix from 30 patients. twenty-two patients (group i) had an acute purulent appendicitis (three with perforation), four were classified as slightly inflamed cases (group 2) and four had a normal appendix (group 3). the b. fragilis group of bacteria dominated among th ...1979379991
hemagglutinating activity of fusobacterium nucleatum.gingival isolates of oral fusobacterium nucleatum strains (gram-negative anaerobic fusiform bacilli) have shown the characteristic ability to hemagglutinate a variety of erythrocytes (rbc) of human and animal origin. other members of the genus tested (f. necrophorus, f. varium, and f. mortiferum) displayed little if any ability to hemagglutinate rbc. the hemagglutination (ha) activity could be observed in the f. nucleatum strains with the whole cells and in most instances with sonicated preparat ...1977401771
blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates.soluble sonicate supernatant preparations were made from actinomyces viscosus (atcc 19246), a. naeslundii (atcc 12104), two strains of veillonella alcalescens (strain hv-1 and a human oral isolate), streptococcus sanguis (atcc 10556), s. mutans (strain 6715-t2), bacteroides melaninogenicus (strain k110), and leptotrichia buccalis (isolated from human dental plaque). these supernatants were characterized with reference to their chemical and antigenic components and their biological activity deter ...1976977126
haemagglutinating activity of leptotrichia buccalis cells and their adherence to saliva-coated enamel powder. 19761066115
soluble antigens of leptotrichia buccalis: their reaction with rabbit antisera. 19751068997
unusual cell wall ultrastructure of leptotrichia buccalis.leptotrichia buccalis was examined by transmission electron microscopy. its cell wall structure in generally compatible with that of gram-negative bacteria. however, the scale-like membranous folds associated with the external surface of the outer cell membrane appear to be sufficiently unusual to serve as a useful morphological criterion in the identification of l. baccalis cells.19751150629
comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms.the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ...19921331019
the early colonization of gram-negative anaerobic bacteria in edentulous infants.the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ...19921528621
dna base composition, aerotolerance and enzyme patterns of leptotrichia buccalis.the guanine plus cytosine content of the dna of leptotrichia buccalis varied from 28.4 to 29.5 mol% (three strains). eleven strains examined grew well under anaerobic and microaerobic conditions, but slowly in air in the presence of co2. when examined for preformed enzymes in the apizym complete research kit, positive reactions were obtained for several glucosidases and carboxylic ester hydrolases, and for a few peptidases.19921554484
use of synthetic oligonucleotide dna probes for the identification of different strains of fusobacterium nucleatum.the ability of three different oligonucleotide probes to identify different oral strains of fusobacterium nucleatum was tested. probes 119 and 214 were designed on the basis of known amino acid sequences from the n-terminal end of an outer membrane protein of f. nucleatum strain fev1. probe h 2.1, was a randomly cloned chromosomal dna fragment of 2.1 kbp. the specificities of the probes were examined by testing each of them against a panel of five other species of fusobacterium (six strains), or ...19911837056
use of chemotaxonomy as an aid to differentiate among capnocytophaga species, cdc group df-3, and aerotolerant strains of leptotrichia buccalis.four strains of fastidious gram-negative rods, thought to be capnocytophaga species (formerly cdc group df-1 or bacteroides ochraceus) or cdc group df-3 on the basis of conventional phenotypic criteria, were also analyzed for cellular fatty acid (cfa) composition. it was found that the cfa compositions of these strains were qualitatively incorrect for those taxa. subsequently, it was determined that all four bacteria were in fact aerotolerant strains of leptotrichia buccalis, based on biochemica ...19911939582
leptotrichia buccalis bacteremia in patients with cancer: report of four cases and review.leptotrichia buccalis, an anaerobic gram-negative rod, is part of the normal oral flora and has rarely been isolated from clinical material. we describe four patients with neutropenia and progressive malignancy who had symptomatic l. buccalis bacteremia, and we review an additional four cases from the literature. the mean age of the patients was 31 years (range, 7-73 years), with an equal number of males and females. the number of positive blood cultures in each case ranged from one to four (mea ...19912041949
obligately anaerobic bacterial species isolated from foot-rot lesions in goats.lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ...19902271912
capnophilic and anaerobic bacteremia in neutropenic patients: an oral source.the currently accepted empiric antibiotic therapy for bacterial infections in neutropenic patients may not cover the possibility of capnophilic and anaerobic bacteremia. many of these infections develop in patients with severe mucositis or periodontitis, and the type of organisms recovered also suggests an oral source of infection. we present two cases of bacteremia in neutropenic patients who had been empirically treated with ceftazidime and piperacillin plus amikacin. in the first case a beta- ...19902305182
outer membrane proteins as major antigens of fusobacterium nucleatum.the immunochemical reactions of rabbit polyclonal antibodies directed to different preparations of fusobacterium nucleatum i.e, whole cells, peptidoglycan associated proteins, a peptidoglycan-protein complex and a purified 40 kilodalton (kda) protein, were investigated on outer membrane preparations of fusobacterium species and a restricted number of leptotrichia buccalis after their separation on sodium dodecyl sulphate polyacrylamide gels and electrotransfer to nitrocellulose. all f. nucleatum ...19892627499
a pyrolysis mass spectrometry study of fusobacteria.strains of fusobacteria (143) were examined by pyrolysis mass spectrometry (py-ms) with a horizon instruments pyms 200x. fusobacterium necrogenes, f. necrophorum, f. nucleatum, f. mortiferum, f. varium, f. gonidiaformans, f. naviforme, f. russii and leptotrichia buccalis were discriminated. strains of fusobacteria isolated from tropical ulcers, although similar to f. mortiferum in conventional tests, were discriminated from each of these species in py-ms. identification of 416 spectra to species ...19892926794
selective suppression of the anaerobic oropharyngeal microflora with local metronidazole.anaerobic microorganisms of the normal oropharyngeal flora have been shown to be the main pathogens in orofacial infections of odontogenic origin. reduction of the density of anaerobes in the oral cavity as a prophylactic measure before oral surgery may be a rational way to reduce the frequency of post-operative infections. this report describes a local antibiotic regimen that can reduce anaerobic pathogens in the oral cavity. over a period of 7 days, 10 healthy individuals applied locally 1.5-2 ...19872948544
[bactericidal activity examination (mbc) of glutaric aldehyde on anaerobic non-sporing bacteria].sensibility (mbc) of 97 strains of non-sporing anaerobes separated from clinical materials coming from oral cavity to glutaric aldehyde (koch light lab) has been examined. the experiments have been carried out by the suspension method adapting it properly to the examinations of anaerobes. 72 hours' cultures in enriched thioglycolate broth containing 10(9) of living cells of anaerobes in 1 ml have been used as inoculum. mbc results were read off after 7 days' incubation at temperature of 37 degre ...19883152063
[sterinole and cetylpirydine chloride influence on non-sporulated anaerobic bacteria of oral cavity].sensibility (mbc--minimal bactericidal concentration) of 97 strains of non-sporulated anaerobic bacteria separated from clinical material from oral cavity to sterinole (polfa) and cetylpirydine chloride has been examined. experiments have been carried out by means of suspension method adapted appropriately to the tests on non-sporulated anaerobes. 72 hours' cultures of strains containing 10(9) of live bacterial cells in 1 ml have been used as inoculum. mbc readings have been performed after 7 da ...19883270591
kinin generation in the gingival inflammatory response to topically applied bacterial lipopolysaccharides.a water-soluble lipopolysaccharide from salmonella enteritidis and a phenol-soluble lipopolysaccharide from leptotrichia buccalis were applied topically to the healthy marginal gingivae of beagle dogs. saline was applied to contralateral areas as an internal control. increases in vascular permeability were monitored by measurement of gingival fluid, and the collected gingival fluid samples were assayed for kininogenase and kinin activities. both lipopolysaccharides induced an inflammatory respon ...19863511110
oligosaccharides obtained by partial hydrolysis of lipopolysaccharides from leptotrichia buccalis.lipopolysaccharides (lps) from leptotrichia buccalis, strains l11, atcc 14201 and atcc 19616 were partially degraded with 1% acetic acid. the acid soluble polysaccharides were subjected to gel filtration. these experiments showed that the carbohydrate moiety of lps from atcc 14201 and atcc 19616 were similar, consisting of a core oligosaccharide unsubstituted by 0-side chains containing galactose, glucose and d-glycero-d-manno-heptose as neutral sugars. the core analogous fraction isolated from ...19853864216
identification of fusobacteria in a routine diagnostic laboratory.a scheme for differentiating fusobacterium spp. and leptotrichia spp. from bacteroides spp. was devised after examining 114 strains of fusobacteria and asaccharolytic bacteroides (17 reference strains and 97 clinical isolates). sensitivity to a 300 micrograms/ml plate of phosphomycin and an acid reaction on a lysine plate were found to be reliable for differentiating fusobacterium spp. and l. buccalis from bacteroides. using a short set of simple cultural and biochemical tests, isolates could be ...19853900021
the classification and differentiation of the anaerobic gram-negative rods.within the family bacteroidaceae differentiation of the genera leptotrichia, fusobacterium and bacteroides still depends largely on the accumulation of certain fermentation products. the application of newer techniques, both genetical and chemical is leading to a reappraisal of the current classification. so far attention has focussed on the genus bacteroides and has been concerned with the status of b. fragilis and related organisms and the description of a number of new species within the b. r ...19853906857
leptotrichia buccalis bacteremia in neutropenic children.two new cases of leptotrichia buccalis bacteremia in seriously ill patients were described. the anaerobic, gram-negative microorganism l. buccalis was isolated from blood cultures of two children with severe leukopenia. anaerobic organisms should be taken into account when a standard protective antibiotic chemotherapy is considered in the immunosuppressed host.19854031045
[leptotrichia buccalis--culture, biochemistry and antibiotics]. 19744154653
[immunelectrophoretic studies of the action of bacteroides, fusobacterium, leptotrichia, and sphaerophorus species on human plasma proteins]. 19714251689
[septicemia due to leptotrichia vaginalis]. 19684300965
isolation of a phenol-soluble endotoxin from leptotrichia buccalis. 19734513115
bacteremia after tooth extractions studied with the aid of prereduced anaerobically sterilized culture media.both prereduced molten agar and broth and aerobic molten agar and broth were inoculated with blood samples collected from patients with periodontitis, but in otherwise good health, both before and after extraction of two or more teeth. postoperative blood samples from 23 of 25 patients sampled yielded anaerobic and facultative species. colony counts from nine samples yielded from less than 1 to over 100 colonies per ml of blood. organisms detected were species belonging to the genera bacteroides ...19744598226
[leptotrichia (leptothrix) vaginalis]. 19724671249
studies on oral filamentous bacteria. ii. serological relationships within the genera actinomyces, nocardia, bacterionema and leptotrichia. 19674965337
in vitro evaluation of actinobolin as an antibiotic for the treatment of periodontal disease.actinobolin was evaluated in vitro by a paper disc-agar diffusion method for inhibitory activity against mixed microbial cultures obtained from patients with periodontal disease and against pure bacterial cultures tentatively identified as strains of bacteroides melaninogenicus, fusobacterium fusiforme, leptotrichia buccalis, and veillonella parvula. every culture tested was inhibited to some degree by actinobolin. these observations suggest that actinobolin may be effective in the treatment of ...19725059624
the susceptibility of bacteroides, fusobacterium leptotrichia, and sphaerophorus strains to rifampicin. 19725068353
a study of leptotrichia buccalis. ii. biochemical and physiological observations. 19655213005
the biological activity of leptotrichia buccalis endotoxin. 19665335235
ultrastructure of leptotrichia buccalis. 19695787001
chemical characteristics of leptotrichia buccalis endotoxin. 19665970130
an anaerobic oral filamentous organism possibly related to leptotrichia buccalis. 1. morphology, some physiological and serological properties. 19676031368
studies on the oral filamentous bacteria. i. cell wall composition of actinomyces, nocardia, bacterionema and leptotrichia. 19676074011
an anaerobic oral filamentous organism possibly related to leptotrichia buccalis. 2. composition of cell walls. 19676083389
rapid method that aids in distinguishing gram-positive from gram-negative anaerobic bacteria.several species of anaerobic bacteria display variable gram stain reactions which often make identification difficult. a simple, rapid method utilizing a 3% solution of potassium hydroxide to distinguish between gram-positive and gram-negative bacterial was tested on 213 strains of anaerobic bacteria representing 19 genera. the gram stain reaction and koh test results were compared with the antibiotic disk susceptibilities (vancomycin and colistin) the preliminary grouping of anaerobic bacteria. ...19816165736
[septicemia due to leptotrichia buccalis in an immuno-suppressed patient].the authors report a case of septicemia due to leptotrichia buccalis in a patient with underlying lymphatic leukemia. it is the second case reported in the literature. this observation shows, once again, that bacteria known as "non virulent" can exhibit pathogenicity in immunosuppressed patients.19826291160
anaerobes as normal oral flora.the human mouth provides a suitable habitat for numerous bacterial genera. anaerobic genera or genera that include anaerobic members found in the oral cavity are actinomyces, arachnia, bacteroides, bifidobacterium, eubacterium, fusobacterium, lactobacillus, leptotrichia, peptococcus, peptostreptococcus, propionibacterium, selenomonas, treponema, and veillonella. the incidence of anaerobes varies with age of the individual and with specific sites sampled. in edentulous infants, the incidence of a ...19846372039
bacteriology of experimental gingivitis in children.children are more resistant to gingivitis than are adults. to determine possible differences in their periodontal floras, an experimental gingivitis study, identical in design to one reported earlier with young adults, was conducted with four 4- to 6-year-old children. the incidence of sites that developed gingival index scores of 2 in children was less than one-third of the incidence observed in adults. the composition of the flora of each child was statistically significantly different from th ...19846480100
infective endocarditis due to leptotrichia buccalis: a case report.a patient with down's syndrome presented with infective endocarditis due to leptotrichia buccalis. the source of the infection was not detected, but the predisposing factor was a complex cardiac malformation. the disease followed a subacute course, had a number of immunologic manifestations and was successfully treated with a 28-day course of penicillin g, given intravenously. l. buccalis has never been reported before as a cause of endocarditis.19846692239
purification and quantitative chemical analysis of cell wall peptidoglycans of leptotrichia buccalis.peptidoglycans of leptotrichia buccalis atcc 14201 and atcc 19616 were isolated by extraction with sodium dodecyl sulfate and subsequent digestion of the sodium dodecyl sulfate-insoluble residue with proteases and alpha-amylase. cell wall fractions obtained by sodium dodecyl sulfate extraction and protease digestion were highly contaminated by a glucose polymer. the polyglucose was removed by alpha-amylase treatment, and the peptidoglycans were left behind. analyses with amino acids and amino su ...19836822410
comparative ultrastructure of certain actinomyces species, arachnia, bacterionema and rothia.a comparative ultrastructural study was carried out on several species of acinomyces and related gram-positive rods including arachnia, bacterionema, rothia and leptotrichia. a total of 52 well characterized strains were examined by transmission electron microscopy. particular attention was paid to the ultrastructure of the cell periphery, that is the plasma membrane, periplasmic space, the cell wall per se and extramural structures, including surface "fuzz". in addition, the ultrastructure of v ...19806928945
[yeast-like fungi and leptotrichia of the oral mucosa]. 19806929126
regulation of lactic acid production by leptotrichia buccalis. 19816937247
the development of an altered gingival crevicular microflora in the alloxan-diabetic rat.the microflora of the rat gingival crevice were examined at various time intervals after inducing experimental diabetes. a variety of gram-positive and gram-negative cocci and short rods were isolated from the normal rat crevice. within a week after alloxan administration, leptotrichia buccalis was detected for the first time. other features in the diabetic animals included an increased frequency of isolation of proteus spp., lactobacillus spp. and decrease of escherichia coli. occasionally, a d ...19826953937
serious infection with leptotrichia buccalis. report of a case and review of the literature.leptotrichia buccalis is an obligately anaerobic, gram-negative rod classified as a member of the family bacteroidaceae. organisms found in this family (bacteroides and fusobacteria) are frequent causes of severe infection in human beings. l. buccalis is a constituent of normal oral flora. leptotrichia has rarely been recovered from mixed infections primarily of the head and neck. it has not previously been reported as a cause of serious infection in man. we report a case of cavitary pneumonia a ...19807001901
endotoxins of gram-negative anaerobic bacteria.the author reviews the data available on endotoxins of fusobacterium, sphaerophorus, leptotrichia, and veillonella species, and reports his own findings with lipopolysaccharides extracted from bacteroides fragilis strains by the trichloroacetic acid method. the latter extracts showed a very low toxicity for mice after i.v. injection of dosages of less than or equal to 500 micrograms and a slight leucopenic effect in mice after intraperitoneal injection of 50 micrograms.19807005091
[septicemia due to leptotrichia buccalis in an immuno-suppressed patent (author's transl)].the authors report a case of septicemia due to leptotrichia buccalis in a patient with underlying lymphatic leukemia. it is the second case reported in the literature. this observation shows, once again, that bacteria known as "non virulent" can exhibit pathogenicity in immunosuppressed patients.19827048217
chemical composition, ultrastructure and some serological properties of lipopolysaccharides from leptotrichia buccalis.lipopolysaccharide (lps) was extracted with aqueous phenol from leptotrichia buccalis strains l 11, atcc 14201 and atcc 19616. virtually all the lps was found in the water phase. lps could also be extracted with phenol/chloroform/petroleum ether, but not with cold trichloroacetic acid. all lps preparations contained d-glycero-d-manno-heptose, galactose, glucose, glucosamin, n-dodecanoate, 3-hydroxy-n-tetradecanoate and phosphorus. in addition lps from l11 contained 2-keto-3-deoxyoctonate and an ...19827148458
isolation by gel filtration and ion-exchange chromatography of a carbohydrate-rich lps from phenol-water extracts of leptotrichia buccalis strain l11. 19827168318
comparative in vitro activity of new beta-lactam antibiotics against anaerobic bacteria.several new beta-lactam antimicrobial agents have been introduced in the last few years. in this investigation, the in vitro activities of several recently introduced cephalosporins (cefoperazone, cefotaxime, ceftazidime, and ceftizoxime), moxalactam, and n-formimidoyl thienamycin were compared with those of cefoxitin, clindamycin, and metronidazole against 203 strains of anaerobic bacteria. at achievable serum levels, all of the antimicrobial agents were active against essentially 100% of the s ...19817325628
neuraminidase production by bacteroidaceae.the production of neuraminidase (ec 3.2.1.18) by 77 strains of bacteroidaceae was investigated by techniques previously used to study neuraminidase production by clostridia. conditions for culture and assay of bacteroides fragilis neuraminidase were characterised. the enzyme is predominantly cell associated; it is not calcium dependent and the ph optimum for its production is c. 4.5. most neuraminidase-positive bacteroides strains produced the enzyme well in the test media but a few strains fail ...19817463468
leptotrichia sanguinegens sp. nov., a new agent of postpartum and neonatal bacteremia. 19957548563
leptotrichia buccalis bacteremia in patients treated in a single bone marrow transplant unit.we describe four cases of bacteremia due to leptotrichia buccalis (an organism that is part of the normal human oral flora) that occurred in a bone marrow transplant unit over a 3-month period. all of the patients were neutropenic, all had mucositis or esophagitis, and all were receiving antimicrobial prophylaxis with ciprofloxacin and vancomycin (drugs to which leptotrichia is resistant). one patient died of adult respiratory distress syndrome; the others had minimal symptoms. pulsed field gel ...19957795070
the surface structure of leptotrichia buccalis.leptotrichia buccalis shows a mosaic of surface structure on its outer membrane consisting of curved ridges 35 mm high and 22 nm apart, and erect on that surface. fimbriae (common pili) are not present and nor is an s layer. the flap-like ridges consist of strings of macromolecules radiating from the cell surface. this ridge structure is not soluble in any of the usual chaotropes and can only be released when the outer membrane has been damaged or dispersed by extracting envelope preparations wi ...19947912643
the oral gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. the bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). during the primary period, 2 samples were collected from each child: a sample with d ...19947936718
the most frequently isolated gram-negative anaerobes in saliva and subgingival samples taken from young women.stimulated saliva and a pooled subgingival sample from the first molars were collected from 30 caucasian women (mean age 30 years) for the presence of gram-negative anaerobic bacteria. the samples were inoculated on nonselective and selective media. the gram-negative anaerobic species occurring in the oral cavity of more than 50% of the subjects were selected for further analysis. fusobacterium nucleatum and prevotella melaninogenica were not recovered from subgingival sites without a simultaneo ...19948008430
endocarditis caused by a leptotrichia buccalis-like bacterium in a patient with a prosthetic aortic valve.the isolation of a leptotrichia buccalis-like bacterium from the blood of a patient with endocarditis associated with a prosthetic aortic valve is reported. the organism was isolated from different blood cultures. it was similar but not completely identical to leptotrichia buccalis. the microbiological identification was confirmed independently by three laboratories, including two reference institutions. the case and the characteristics of the microorganism are reported.19938513815
human root caries: microbiota in plaque covering sound, carious and arrested carious root surfaces.the plaque microbiota covering sound or carious root surfaces were studied and compared with that covering arrested root caries lesions. from each of these categories five extracted teeth were examined. the experimental design of the study allowed us to relate the qualitative and quantitative microbial composition to the degree of integrity of the root surface. plaque was sampled by a newly developed 'mowing' technique. plaque samples were cultured anaerobically on nonselective columbia blood ag ...19958521441
bacteraemia with leptotrichia buccalis: report of a case and review of the literature.we report here on a new case of l. buccalis bacteraemia. to our knowledge 16 other cases of l. buccalis bacteraemia have been reported in the literature, most of them in neutropenic patients. however l buccalis endocarditis does occasionally occur in non-neutropenic patients. the identification of l. buccalis is based on well known phenotypic features and confirmed by the detection of a large peak of lactic acid by gas chromatography for non volatile organic acids. l. buccalis is sensitive to a ...19968858893
hepatic abscess likely related to leptotrichia buccalis in an immunocompetent patient.we present the case of a 64-year-old previously healthy man admitted for long evoluating fever. buccal examination showed severe parodontitis. blood cultures were positive for leptotrichia buccalis and computed abdominal tomography discovered a hepatic abscess. so far, only exceptional cases of systemic diseases associated with leptotrichia buccalis infections have been described in immunocompetent patients. thus, in cases of severe parodontitis and long evoluating fever, attention must be paid ...19968938638
evaluation of the bbl crystal anaerobe identification system.the bbl crystal anaerobe (anr) identification system was evaluated, and the results were compared with those from conventional anaerobic methods. we tested 322 clinically significant anaerobic bacteria according to the manufacturer's instructions. the system identified correctly 286 of 322 (88.8%) of the anaerobic bacteria tested. of these, 263 of 322 (81.7%) were identified correctly on initial testing and 49 were identified correctly only to the genus level; on repeat testing, 23 of 49 (46.9%) ...19979399517
clinical and microbiological features of refractory periodontitis subjects.the purpose of this investigation was to compare the clinical parameters and the site prevalence and levels of 40 subgingival species in successfully treated and refractory periodontitis subjects. 94 subjects received scaling and root planing and if needed, periodontal surgery and systemically administered tetracycline. 28 refractory subjects showed mean full mouth attachment loss and/or > 3 sites showing attachment loss > 2.5 mm within 1 year post-therapy. 66 successfully treated subjects showe ...19989495617
bacteremia caused by a novel isolate resembling leptotrichia species in a neutropenic patient.we report a case of leptotrichia species bacteremia in a patient undergoing treatment for acute myelogenous leukemia. like previously reported leptotrichia species, this is a gram-variable, pleomorphic rod that is catalase negative and utilizes glucose and sucrose. however, it is more fastidious than previously reported isolates of leptotrichia and may represent a novel species.199910325382
evaluation of the oxyrase oxyplate anaerobe incubation system.the oxyrase oxyplate anaerobe incubation system was evaluated for its ability to support the growth of clinically significant anaerobic bacteria previously identified by the anaerobe reference laboratory at the centers for disease control and prevention. the results were compared with those obtained with conventional anaerobe blood agar plates incubated in an anaerobe chamber. we tested 251 anaerobic bacterial strains. plates were read at 24, 48, and 72 h; growth was scored by a numerical coding ...200010655335
early microbial colonization of permucosal implants in edentulous patients.in edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ...198710872401
risk factors for anaerobic bloodstream infections in bone marrow transplant recipients.the incidence of anaerobic bloodstream infections (bsi) in patients who underwent bone marrow transplantation (bmt) recently increased at our institution. a retrospective case-control study of patients undergoing bmt from january 1995 through december 1998 was performed to determine the microbiological characteristics, epidemiology, and outcome of anaerobic bsi and to identify independent risk factors for infection. anaerobic bsi occurred in 23 patients, for a rate of 4 bsis per 100 bmt procedur ...200111438899
bacteremia due to leptotrichia trevisanii sp. nov.a thin, filamentous, non-motile, aerotolerant, anaerobic, gram-negative bacterium was isolated from the blood of a 46-year-old man who was diagnosed as having acute myeloid leukemia. the organism had a positive catalase reaction but was negative in indole and oxidase tests. a commercially available system failed to identify the bacterium, but 16s rrna gene sequencing showed it to be most closely related (97% similarity) to a recently isolated leptotrichia sp. the dna base composition was 29.7% m ...200111783691
characterization of some strains from human clinical sources which resemble "leptotrichia sanguinegens": description of sneathia sanguinegens sp. nov., gen. nov.three strains of a gram-negative, blood or serum requiring, rod-shaped bacterium recovered from human clinical specimens were characterised by phenotypic and molecular taxonomic methods. comparative 16s rrna gene sequencing showed the unknown rod-shaped strains are members of the same species as some fastidious isolates recovered from human blood specimens and previously designated "leptotrichia sanguinegens". based on phylogenetic and phenotypic evidence, it is proposed that the isolates from h ...200111822670
16s-23s rdna internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus fusobacterium.the 16s-23s rdna internal transcribed spacer (its) regions of all currently defined fusobacterium species and related taxa such as leptotrichia buccalis, sebaldella termitidis and streptobacillus moniliformans, were analysed to examine inter- and intraspecies as well as subspecies relationships. for the its-amplification, a new eubacterial universal primer pair was designed and used. the majority of the fusobacterium strains, along with l. buccalis showed one major, and two to three weaker, dist ...200211931161
leptotrichia amnionii sp. nov., a novel bacterium isolated from the amniotic fluid of a woman after intrauterine fetal demise.a novel bacterium was isolated and characterized from the amniotic fluid of a woman who experienced intrauterine fetal demise in the second trimester of pregnancy. the bacterium was a slow-growing, gram-negative anaerobic coccobacillus belonging to the genus leptotrichia: unlike leptotrichia sanguinegens, the isolate did not grow in chopped-meat glucose broth or on sheep blood agar upon subculturing. the isolate was characterized by sequencing and analyzing its 16s rrna gene. the 1,493-bp 16s ri ...200212202577
purification and some properties of phospho-beta-galactosidase from the gram-negative oral bacterium leptotrichia buccalis atcc 14201.phospho-beta-galactosidase (p-beta-gal; ec 3.2.1.85) is induced during growth of leptotrichia buccalis atcc 14201 on lactose and lactulose. the enzyme has been purified to electrophoretic homogeneity (m(r) approximately 53 kda, pi approximately 4.8), and kinetic parameters have been determined using the chromogenic analog o-nitrophenyl-beta-d-galactopyranoside-6-phosphate as substrate. both atp and galactose-6-phosphate are inhibitors of p-beta-gal activity. microsequence analysis has identified ...200212351228
correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese.the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ...200212483235
usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles.due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ...200312734240
[study of a pigmented variety of leptotrichia innominata]. 195513403384
a study of leptotrichia buccalis. 195713416200
isolation of leptotrichia buccalis and fusobacterium species from oral material. 195713483608
isolation of leptotrichia buccalis. 195813575396
the morphology of leptotrichia species. 195813611186
cell-wall composition of leptotrichia spp. 195913657058
the classification of organisms termed leptotrichia (leptothrix) buccalis. iii. growth and biochemical characteristics of bacterionema matruchotil. 196113705432
the classification of organisms termed leptotrichia (leptothrix) buccalis. i. review of the literature and proposed separation into leptotrichia buccalis trevisan, 1879 and bacterionema gen. nov., b. matruchotti (mendel, 1919) comb. nov. 196113705433
the classification of organisms termed leptotrichia (leptothrix) buccalis. ii. reproduction of bacterionema matruchotii. 196113705434
the classification of organisms termed leptotrichia (leptothrix) buccalis. iv. physiological and biochemical characteristics of bacterionema matruchotii. 196113716258
serological specificity of acid-soluble antigens of bacterionema matruchottii.sibal, louis r. (university of illinois college of medicine, chicago), arthur v. kroeger, dan kumarich, and esther meyer. serological specificity of acid-soluble antigens of bacterionema matruchottii. j. bacteriol. 83:811-818. 1962.-the serological specificity of acid-soluble antigens extracted from the oral, filamentous organism, bacterionema matruchottii, was demonstrated by the microprecipitin reaction. there was no evidence of cross-reaction with antisera prepared against leptotrichia buccal ...196213912471
isolation, growth requirements, and pathogenicity for rabbits of leptotrichia dentium. 196213919403
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