| the influence of age on the susceptibility of bulls to campylobacter fetus subsp venerealis. | observations were carried out on 22 hereford bulls to determine the age at which they bacame carriers of c. fetus. during preliminary investigations, 5 bulbs were repeatedly exposed to the organism by artificial or natural means from the age of 18 months. all animals became longterm carriers on reaching an age of between 40 and 70 months. prior to this, a temporary carrier state lasting from 4 to 29 days was produced in 4 bulls. in a subsequent experiment, 17 bulls of 3 different age groups were ... | 1975 | 1167135 |
| studies on veneral transmission of campylobacter fetus by immunised bulls. | temporary contamination of the penis and prepuce with campylobacter fetus subsp. venerealis and campylobacter fetus subsp. venerealis biotype intermedius may occur when immunised bulls mate with infected heifers. however, 2 experiments showed that this contamination was not a significant cause of genital infection in susceptible heifers that subsequently mated with bulls. | 1975 | 1243730 |
| prevalence of bovine campylobacteriosis in indigenous cattle of three states in nigeria. | a survey of bovine campylobacteriosis in breeding bulls and cows was carried out in the states of kaduna, kano and borno. six hundred and eighty nine cattle composed of 585 and 104 breeding bulls and cows respectively were sampled. campylobacter fetus subsp. venerealis was isolated from 12 bulls while campylobacter fetus subsp. fetus was isolated from three of them. campylobacter fetus subsp. fetus was isolated from four cows while campylobacter fetus subsp. veneralis was isolated from one cow. ... | 1991 | 1763437 |
| diagnosis by elisa of bovine abortion due to campylobacter fetus. | an enzyme-linked immunosorbent assay (elisa) was developed to detect antigen-specific secretory iga antibody in bovine vaginal mucus after abortion due to campylobacter fetus subsp venerealis. abortions were diagnosed by isolating the organism from 8 foetuses and/or foetal membranes and by histopathology. vaginal mucus was collected from 7 cows shortly after abortion. all showed a high level of iga antibody in their vaginal mucus when they were compared with an uninfected control group. the new ... | 1991 | 1953551 |
| multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. | the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ... | 1991 | 1993755 |
| control of campylobacter fetus in artificially contaminated bovine semen by incubation with antibiotics before freezing. | fresh, diluted semen containing 1.55 x 10(6) cfu/ml of campylobacter fetus subsp. venerealis was incubated with 500 iu of penicillin, 500 micrograms of streptomycin, 160 micrograms of lincomycin and 300 micrograms of spectinomycin per ml at 35 degrees c for 0, 1, 2, 5, 10, 20 or 40 minutes. the semen was cooled to 5 degrees c, packaged in 0.25 ml french straws and then frozen in liquid nitrogen for 2 weeks. immediately after thawing and removal of the antibiotics by centrifugation semen samples ... | 1990 | 2306605 |
| antigenic and restriction enzyme analysis of isolates of campylobacter fetus subsp venerealis recovered from persistently infected cattle. | thirty-two isolates of campylobacter fetus subsp venerealis were obtained from 1 bull and 4 heifers with experimentally induced infection. when whole-cell antigens of isolates were cross titrated with antisera to the infecting strain, isolates from 3 heifers had limited antigenic variation, whereas whole-cell antigens of isolates from 2 cattle (the bull and a heifer) differed serologically from those of the infecting strain. changes were detected specifically in 6 heat-labile antigens. of the 6 ... | 1989 | 2548417 |
| acid and alkaline campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits. | acid and alkaline extracts from several campylobacter strains were used as antigens in enzyme immunoassay (eia). antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different campylobacter subspecies. for the preparation of the extracts, three campylobacter fetus subsp. venerealis strains, three campylobacter fetus subsp. fetus strains, four campylobacter coli, and five campylobacter jejuni strains were used. the acid extracts of campyloba ... | 1987 | 3296648 |
| vaccination of pregnant guinea pigs with campylobacter fetus: effects of antigen dose, campylobacter strain, and adjuvant type. | for the purpose of developing and improving vaccines to protect cattle against bovine genital campylobacteriosis, the interaction of vaccine dose, strain(s) of campylobacter fetus, and type of adjuvant was studied in the pregnant guinea pig model. five strains of c fetus were used in the preparation of 20 monovalent vaccines containing various doses of bacteria (0.1 mg to 20 mg, dry weight) in freund incomplete adjuvant, freund complete adjuvant, or aluminum hydroxide gel adjuvant. vaccinal prot ... | 1988 | 3377303 |
| the demonstration of the k-antigen of campylobacter fetus venerealis using a microtitre agglutination test. | using specific antisera prepared against heat-treated and untreated cells in a microtitre system, 7 strains of campylobacter fetus venerealis were examined for the presence of k-antigen. the technique was effective for distinguishing between strains and it was found that 4 out of 7 isolates contained microcapsules, therefore being potentially suitable for vaccine production. | 1987 | 3444618 |
| letter: isolation of campylobacter fetus subsp. venerealis and campylobacter fetus subsp. intermedius from the preputial secretions of bulls. | | 1974 | 4414274 |
| immunisation against bovine vibriosis. vaccination of bulls against infection with campylobacter fetus subsp. venerealis. | | 1974 | 4441328 |
| bovine genital campylobacteriosis (vibriosis): vaccination of experimentally infected bulls. | the therapeutic efficacy of a campylobacter fetus subsp venerealis bacterin was determined in experimentally infected bulls. ten of twelve 5-year-old angus bulls became infected after being infused intrapreputially with c fetus subsp venerealis. of the 10 bulls, 6 were vaccinated with 5 ml of c fetus subsp venerealis vaccine on 2 occasions 4 weeks apart. preputial washings of the vaccinated bulls were culturally negative by the 8th week after primary vaccination. none of the 18 heifers exposed t ... | 1983 | 6194723 |
| the duration of protection against infection with campylobacter fetus subsp. venerealis in immunised bulls. | | 1982 | 7138441 |
| tritrichomonas foetus agglutination tests upon samples collected from cattle: cross-reactions associated with vaccination against campylobacter fetus subsp venerealis. | | 1981 | 7340796 |
| pathogenicity of campylobacter fetus subsp. venerealis in causing infertility in cattle. | | 1980 | 7388595 |
| shift in s-layer protein expression responsible for antigenic variation in campylobacter fetus. | campylobacter fetus strains possess regular paracrystalline surface layers (s-layers) composed of high-molecular-weight proteins and can change the size and crystalline structure of the predominant protein expressed. polyclonal antisera demonstrate antigenic cross-reactivity among these proteins but suggest differences in epitopes. monoclonal antibodies to the 97-kda s-layer protein of campylobacter fetus subsp. fetus strain 82-40lp showed three different reactivities. monoclonal antibody 1d1 re ... | 1993 | 7688715 |
| protein shift and antigenic variation in the s-layer of campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapa homologs. | campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a c. fetus-negative heifer. isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kda s-layer protein. immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kda s-layer protein and other c. fetus s-layer proteins demonstrated antigenic shifts. ... | 1995 | 7721688 |
| studies on inactivation of pathogenic microorganisms in culture media and in bovine semen by photosensitive agents. | the application of three photosensitive agents for disinfection of bovine semen was investigated. bovine microbial pathogens suspended in tissue culture medium and/or pbs and also added to bovine semen were exposed to the photosensitive agents followed by irradiation. hematoporphyrin, hematoporphyrin derivative and thiopyronine were effective against bovine herpes virus-1, bovine viral diarrhoea virus, mycoplasma bovigenitalium, mycoplasma canadense, and ureaplasma diversum in culture media. in ... | 1994 | 8017031 |
| evaluation of cultural methods and selective media for the isolation of campylobacter fetus subsp venerealis from cattle. | | 1994 | 8080409 |
| bactericidal effects of ozone at nonspermicidal concentrations. | a study was conducted to assess the use of ozone (o3) to control pathogens or contaminants of concern to animal breeders and regulatory officials. in separate experiments, samples of fresh bovine semen and pseudomonas aeruginosa, escherichia coli, or campylobacter fetus subsp. venerealis were diluted with antibiotic-free milk (10(6) sperm and 10(6) organisms/ml of diluted semen), exposed in the previous day to a constantly monitored level of 5, 10, 15, or 20 micrograms/ml of o3 for 3-5 min. afte ... | 1995 | 8521350 |
| the campylobacter fetus s layer is not essential for initial interaction with hep-2 cells. | in vitro adherence assays were used to determine whether the s layer mediated interactions between campylobacter fetus subsp. venerealis strains and hep-2 cells. at multiplicity of infection ratios ranging from 0.1:1 through 100:1, quantitation of bacterial adherence by light microscopy revealed that s layer deficient isogenic c. fetus 809k and c. fetus 810k were not less efficient in their attachment to hep-2 cells; either s layer deficient c. fetus strains interacted with hep-2 cells in greate ... | 1998 | 9606907 |
| lectin-binding sites in uterus and oviduct of normal and campylobacter fetus subspecies venerealis-infected heifers. | this study was carried out to investigate the effect of infection with campylobacter fetus subsp. venerealis (cfv) on the pattern of lectin binding in the uterus and oviduct of heifers. cfv persistence was demonstrated by bacterial isolation and immunofluorescence. infected animals showed variations in the lectin binding pattern when compared with control animals. cfv-infected heifers showed an increased expression of galactose and n-acetyl-galactosamine in the endometrial glands (pna and sba bi ... | 1998 | 9615192 |
| campylobacter fetus diagnosis: direct immunofluorescence comparing chicken igy and rabbit igg conjugates. | in argentina bovine genital campylobacteriosis is routinely diagnosed by direct immunofluorescence test. generally, the hyperimmune sera used for this test are obtained from rabbits and less often from goats. in this work, a chicken egg yolk immunoglobulin (igy) extract was conjugated and its ability to detect campylobacters with the regular conjugate prepared with rabbit sera was comparatively evaluated. both conjugates were independently evaluated by two laboratories, named "azul" (lab a) and ... | 2001 | 11565050 |
| effect of transport enrichment medium, transport time, and growth medium on the detection of campylobacter fetus subsp. venerealis. | the combination of medium and growth conditions, including transport enrichment medium (tem), transport time, tem incubation time, and growth medium, that best support campylobacter fetus subsp. venerealis while inhibiting contaminants was studied. the 3 tems evaluated, weybridge, cary-blair, and 0.85% saline solution, were inoculated with preputial smegma spiked with c. fetus subsp. venerealis and held in the laboratory for 4 or 24 hours before inoculation onto growth medium. the effect of over ... | 2002 | 12680641 |
| investigation of bovine venereal campyloacteriosis in beef cow herds in new zealand. | to determine regional prevalences of beef cow herds in new zealand positive for campylobacter fetus subsp venerealis antibodies in samples of vaginal mucus tested using an immunoglobulin (ig) a enzyme-linked immunosorbent assay (elisa), and to examine the suitability of the iga elisa for detecting infection with c. fetus subsp venerealis under field conditions in new zealand. | 2005 | 15731834 |
| genetic divergence of campylobacter fetus strains of mammal and reptile origins. | campylobacter fetus is a gram-negative bacterial pathogen of both humans and animals. two subspecies have been identified, campylobacter fetus subsp. fetus and campylobacter fetus subsp. venerealis, and there are two serotypes, a and b. to further investigate the genetic diversity among c. fetus strains of different origins, subspecies, and serotypes, we performed multiple genetic analyses by utilizing random amplification of polymorphic dna (rapd), pulsed-field gel electrophoresis (pfge), and d ... | 2005 | 16000457 |
| transport media for campylobacter fetus venerealis. | | 1982 | 16030854 |
| [campylobacter as a venereal disease in cattle]. | since the introduction of ai, venereal diseases caused by tritrichomonas fetus and campylobacter fetus subsp. venerealis haved been eradicated in the netherlands. campylobacter fetus subsp. fetus can cause sporadic abortion and early embryonic death. when natural breeding is practised, venereal diseases must be included in the list of differential diagnoses of fertility problems. a case study of a campylobacter fetus subsp. fetus infection is described. | 2005 | 16047745 |
| a multiplex polymerase chain reaction to detect and differentiate campylobacter fetus subspecies fetus and campylobacter fetus -species venerealis: use on uk isolates of c. fetus and other campylobacter spp. | subspeciation of campylobacter fetus subsp. fetus (cff) and campylobacter fetus subsp. venerealis (cfv) is important for international animal import regulations. phenotyping can be unreliable, and genotyping by techniques like pulsed field gel electrophoresis is difficult in routine diagnostic laboratories. a pcr subspeciation technique has been reported [aust vet j (1997) 75, 827]; we aimed to develop this pcr and investigate its use on uk c. fetus isolates. | 2005 | 16162226 |
| clonal nature of campylobacter fetus as defined by multilocus sequence typing. | campylobacter fetus can be divided into the subspecies c. fetus subsp. fetus and c. fetus subsp. venerealis. c. fetus subsp. fetus causes sporadic infections in humans and abortion in cattle and sheep and has been isolated from a variety of sites in different hosts. c. fetus subsp. venerealis is host restricted, being isolated mainly from the genital tracts of cattle, and is the causative agent of bovine genital campylobacteriosis. despite differences in niche preference, microbiological subspec ... | 2005 | 16333072 |
| comparison of culture and a novel 5' taq nuclease assay for direct detection of campylobacter fetus subsp. venerealis in clinical specimens from cattle. | a campylobacter fetus subsp. venerealis-specific 5' taq nuclease pcr assay using a 3' minor groove binder-dna probe (taqman mgb) was developed based on a subspecies-specific fragment of unknown identity (s. hum, k. quinn, j. brunner, and s. l. on, aust. vet. j. 75:827-831, 1997). the assay specifically detected four c. fetus subsp. venerealis strains with no observed cross-reaction with c. fetus subsp. fetus-related campylobacter species or other bovine venereal microflora. the 5' taq nuclease a ... | 2006 | 16517880 |
| prevalence of genital campylobacteriosis and trichomonosis in crossbred breeding bulls kept on zero-grazed smallholder dairy farms in the tanga region of tanzania. | a survey to demonstrate the presence or absence of genital campylobacteriosis and trichomonosis in cross-bred breeding bulls kept under smallholding dairy farms in the tanga region of tanzania was carried out during the period of january-june 1996. sheath washings, swabs and preputial scrapings were collected from 58 randomly selected bulls. campylobacter fetus subsp. venerealis was demonstrated in 3/58 (5.1%) and tritrichomonas foetus in 0/58 (0%) of all bulls tested. bull-level variables of le ... | 2005 | 16642720 |
| global detection and identification of campylobacter fetus subsp. venerealis. | bovine genital campylobacteriosis caused by campylobacter fetus subsp. venerealis (cfv) is a genital infection that threatens the cattle industry. detection and identification of cfv are key factors in control programmes. trade regulations should be based on scientifically and internationally accepted methods of detection and identification of cfv. such methods are described in the world organisation for animal health (oie) manual of diagnostic tests and vaccines for terrestrial animals. a study ... | 2005 | 16642772 |
| a new antibiotic combination for frozen bovine semen 1. control of mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus. | systematic evaluations of new combinations of antibiotics for the control of bovine mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus in a bovine frozen semen process were made. these organisms were standardized to 10(5) to 10(6) colony forming unit (cfu) and inoculated into each ml of raw semen. antibiotics in a final volume of 0.02 ml were added to each ml of the raw semen and were contained at the same concentration in the nonglycerol portion of the extend ... | 1988 | 16726381 |
| evaluation of direct fluorescent antibody test for the diagnosis of bovine genital campylobacteriosis. | the direct fluorescent antibody test (dfat) for the diagnosis of bovine genital campylobacteriosis was assessed for its detection limit, observer effect, sensitivity and specificity. in addition, the specificity of the fluorescent conjugate was tested against campylobacter sp, arcobacter sp, helicobacter sp, e. coli and other bacteria from the preputial flora. ten - fold dilutions of c. fetus subsp. venerealis nctc 10354 in pbs or preputial washings with or without centrifugation were used. all ... | 2002 | 17063594 |
| discovery of insertion element iscfe1: a new tool for campylobacter fetus subspecies differentiation. | the species campylobacter fetus is divided into the subspecies c. fetus subsp. venerealis (cfv) and c. fetus subsp. fetus (cff). cfv is the causative agent of bovine genital campylobacteriosis, a highly contagious venereal disease that may lead to serious reproductive problems, including sterility and abortion. in contrast, cff can be isolated from the gastrointestinal tract of a wide range of host species, is associated with abortion in sheep and cattle, and can also be isolated from local and ... | 2007 | 17697006 |
| genomic analysis of campylobacter fetus subspecies: identification of candidate virulence determinants and diagnostic assay targets. | campylobacter fetus subspecies venerealis is the causative agent of bovine genital campylobacteriosis, asymptomatic in bulls the disease is spread to female cattle causing extensive reproductive loss. the microbiological and molecular differentiation of c. fetus subsp. venerealis from c. fetus subsp. fetus is extremely difficult. this study describes the analysis of the available c. fetus subsp. venerealis azul-94 strain genome (approximately 75-80%) to identify elements exclusively found in c. ... | 2009 | 19422718 |
| a genomic island defines subspecies-specific virulence features of the host-adapted pathogen campylobacter fetus subsp. venerealis. | the pathogen campylobacter fetus comprises two subspecies, c. fetus subsp. fetus and c. fetus subsp. venerealis. although these taxa are highly related on the genome level, they are adapted to distinct hosts and tissues. c. fetus subsp. fetus infects a diversity of hosts, including humans, and colonizes the gastrointestinal tract. in contrast, c. fetus subsp. venerealis is largely restricted to the bovine genital tract, causing epidemic abortion in these animals. in light of their close genetic ... | 2010 | 19897645 |
| two novel antibiotic resistance genes, tet(44) and ant(6)-ib, are located within a transferable pathogenicity island in campylobacter fetus subsp. fetus. | new tetracycline and streptomycin resistance genes, tet(44) and ant(6)-ib, were identified in campylobacter fetus subsp. fetus within a transferable pathogenicity island that is typically unique to campylobacter fetus subsp. venerealis. the 640-amino-acid tetracycline resistance determinant, tet 44, belongs to a class of proteins that confers resistance to tetracycline and minocycline by ribosomal protection. the 286-amino-acid streptomycin resistance determinant, ant(6)-ib, belongs to a family ... | 2010 | 20479200 |
| development of loop-mediated isothermal amplification and pcr assays for rapid and simple detection of campylobacter fetus subsp. venerealis. | campylobacter fetus is divided into cfv and cff. because cfv causes bovine genital campylobacteriosis, differentiation of the two subspecies is essential to the implementation of efficient cfv control and eradication programs. we have developed lamp and duplex pcr assays for rapid and simple detection of cfv. the lamp assay correctly detected 7 cfv strains and did not detect 53 cff, 35 non-fetus campylobacter and 25 non-campylobacter strains. the pcr assay successfully differentiated the two sub ... | 2010 | 20618686 |
| detection of antibodies specific to campylobacter fetus subsp. venerealis in the vaginal mucus of nigerian breeding cows. | the presence of bovine venereal campylobacteriosis in the lake chad basin of nigeria was investigated using an enzyme-linked immunosorbent assay (elisa) for the detection of iga antibodies specific to campylobacter fetus subsp. venerealis in vaginal mucus (n = 66). iga antibodies specific to c. fetus subsp. venerealis were detected in 7 (11%) vaginal mucus samples. all but one of the iga-positive samples originated from cows belonging to herds with a history of abortion and infertility which sug ... | 2010 | 20857383 |
| interbacterial macromolecular transfer by the campylobacter fetus subsp. venerealis type iv secretion system. | we report here the first demonstration of intra- and interspecies conjugative plasmid dna transfer for campylobacter fetus. gene regions carried by a campylobacter coli plasmid were identified that are sufficient for conjugative mobilization to escherichia coli and c. fetus recipients. a broader functional range is predicted. efficient dna transfer involves the virb9 and vird4 genes of the type iv bacterial secretion system encoded by a pathogenicity island of c. fetus subsp. venerealis. complem ... | 2010 | 21115658 |
| evaluation of pcr assays for the detection of campylobacter fetus in bovine preputial scrapings and the identification of subspecies in south african field isolates. | as a result of the high lability and slow growth of campylobacter fetus subspecies, the laboratory diagnosis of bovine genital campylobacteriosis has always been difficult. this is especially true under south african conditions, where farms are far apart, laboratories are only present in major centres and there are high ambient temperatures. in order to overcome the shortcomings associated with traditional diagnostic methods, the implementation of a molecular assay was sought. this work describe ... | 2010 | 21247013 |
| antimicrobial susceptibility testing of german campylobacter fetus subsp. venerealis isolates by agar disk diffusion method. | campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis and is transmitted by asymptomatic carrier bulls via contaminated semen during artificial insemination. the aim of the present study was to determine the in vitro susceptibility of campylobacter fetus subsp. venerealis isolated from bovine specimens in the years from 2000 to 2009 in germany to antibiotics generally used in semen treatment. the susceptibilities of 50 strains to spectinomycin (10 micr ... | 2011 | 22059289 |
| high prevalence of tritrichomonas foetus infection in asturiana de la montaña beef cattle kept in extensive conditions in northern spain. | bovine trichomonosis (bt) and bovine genital campylobacteriosis (bgc) are sexually transmitted diseases that can be important infectious causes of reproductive failure in extensively managed beef cattle where natural mating is a common practice. however, their prevalence in europe was thought to be insignificant or very low. the purpose of this study was to investigate the prevalence and risk factors associated with bt and bcg in a representative beef cattle breed, asturiana de la montaña (am), ... | 2011 | 22178360 |
| complete genome sequence of type strain campylobacter fetus subsp. venerealis nctc 10354t. | campylobacter fetus subsp. venerealis is the etiologic agent of bovine genital campylobacteriosis, a sexually transmitted disease of cattle that is of worldwide importance. the complete sequencing and annotation of the genome of the type strain c. fetus subsp. venerealis nctc 10354(t) are reported. | 2011 | 21952544 |
| evaluation of a campylobacter fetus subspecies venerealis real-time quantitative polymerase chain reaction for direct analysis of bovine preputial samples. | the detection and subspeciation of campylobacter fetus subsp. venerealis (cfv) from veterinary samples is important for both clinical and economic reasons. campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis, a venereal disease that can lead to serious reproductive problems in cattle, and strict international regulations require animals and animal products to be cfv-free for trade. this study evaluated methods reported in the literature for cfv detec ... | 2012 | 23277694 |
| application of a multiplex pcr assay for campylobacter fetus detection and subspecies differentiation in uncultured samples of aborted bovine fetuses. | campylobacter (c.) fetus (epsilonproteobacteria) is an important veterinary pathogen. this species is currently divided into c. fetus subspecies (subsp.) fetus (cff) and c. fetus subsp. venerealis (cfv). cfv is the causative agent of bovine genital campylobacteriosis, an infectious disease that leads to severe reproductive problems in cattle worldwide. cff is a more general pathogen that causes reproductive problems mainly in sheep although cattle can also be affected. here we describe a multipl ... | 2012 | 23271178 |
| pcr detection of campylobacter fetus subspecies venerealis in smegma samples collected from dairy cattle in fars, iran. | bovine venereal campylobacteriosis, caused by campylobacter fetus subsp. venerealis (cfv), is regarded as one of the major threats to the cattle industry around the world. abortion and infertility are two important reproductive problems in cows infected with c. fetus subsp. venerealis. reports on the presence of cfv are scarce in the cattle, in iran. therefore, the present study was designed to examine the presence of cfv in the reproductive tract of dairy cattle either slaughtered in shiraz aba ... | 2013 | 25568676 |
| prevalence of bovine genital campylobacteriosis and trichomonosis of bulls in northern nigeria. | a survey was conducted to determine the prevalence of campylobacteriosis and trichomonosis, and their concurrence with brucellosis, in cattle in three states of northern nigeria. | 2013 | 23927676 |
| complete genome sequence of campylobacter fetus subsp. venerealis biovar intermedius, isolated from the prepuce of a bull. | campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis, a sexually transmitted disease distributed worldwide. campylobacter fetus subsp. venerealis biovar intermedius strains differ in their biochemical behavior and are prevalent in some countries. we report the first genome sequence for this biovar, isolated from bull prepuce. | 2013 | 23908278 |
| application of a new diagnostic approach to a bovine genital campylobacteriosis outbreak in a saskatchewan beef herd. | a new real-time quantitative polymerase chain reaction (qpcr) test was used to diagnose campylobacter fetus subsp. venerealis infection associated with dramatic reproductive losses in a commercial cow-calf herd. the results were verified with repeated culture, phenotypic characterization of the organism and dna sequencing. this case demonstrates the need for a practical field test for c. fetus subsp. venerealis and the importance of considering this organism as a potential cause of pregnancy fai ... | 2013 | 24082165 |
| diagnosis and management of venereal campylobacteriosis in beef cattle. | bovine venereal campylobacteriosis is caused by campylobacter fetus subsp. venerealis and its glycerine-tolerant variant campylobacter fetus subsp. venerealis biovars intermedius. the disease can be economically important when present in cattle herds, causing poor reproductive performance, embryo mortality and abortion. sensitive and specific diagnostic tests are required in the diagnosis of infection and to inform and monitor disease control. current tests include bacterial culture and fluoresc ... | 2014 | 25428802 |
| draft genome sequences of campylobacter fetus subsp. venerealis bv. venerealis strain b6 and bv. intermedius strain 642-21. | campylobacter fetus subsp. venerealis is an important venereal pathogen. we sequenced the genomes of campylobacter fetus subsp. venerealis bv. venerealis strain b6 and bv. intermedius strain 642-21. the genetic variability of these australian strains will facilitate the study of mechanisms of geographical adaptation of these pathogens that impact livestock. | 2014 | 25278524 |
| first closed genome sequence of campylobacter fetus subsp. venerealis bv. intermedius. | campylobacter fetus subsp. venerealis bv. intermedius is a variant of c. fetus subsp. venerealis, the causative agent of bovine genital campylobacteriosis, a venereal disease associated with abortion and infertility in cattle. we report the first closed whole-genome sequence of this biovar. | 2014 | 24503995 |
| brucellosis, genital campylobacteriosis and other factors affecting calving rate of cattle in three states of northern nigeria. | reproductive diseases limit the productivity of cattle worldwide and represent an important obstacle to profitable cattle enterprise. in this study, herd brucellosis and bovine genital campylobacteriosis (bgc) status, and demographic and management variables were determined and related to predicted calving rate (prcr) of cattle herds in adamawa, kaduna and kano states, nigeria. serum samples, preputial scrapings, questionnaire data, trans-rectal palpation and farm records were used from 271 herd ... | 2015 | 25601264 |
| a novel real-time pcr assay for quantitative detection of campylobacter fetus based on ribosomal sequences. | campylobacter fetus is a pathogen of major concern for animal and human health. the species shows a great intraspecific variation, with three subspecies: c. fetus subsp. fetus, c. fetus subsp. venerealis, and c. fetus subsp. testudinum. campylobacter fetus fetus affects a broad range of hosts and induces abortion in sheep and cows. campylobacter fetus venerealis is restricted to cattle and causes the endemic disease bovine genital campylobacteriosis, which triggers reproductive problems and is r ... | 2016 | 27978826 |
| whole genome sequence analysis indicates recent diversification of mammal-associated campylobacter fetus and implicates a genetic factor associated with h2s production. | campylobacter fetus (c. fetus) can cause disease in both humans and animals. c. fetus has been divided into three subspecies: c. fetus subsp. fetus (cff), c. fetus subsp. venerealis (cfv) and c. fetus subsp. testudinum (cft). subspecies identification of mammal-associated c. fetus strains is crucial in the control of bovine genital campylobacteriosis (bgc), a syndrome associated with cfv. the prescribed methods for subspecies identification of the cff and cfv isolates are: tolerance to 1 % glyci ... | 2016 | 27599479 |
| effect of sample pooling and transport conditions on the clinical sensitivity of a real-time polymerase chain reaction assay for campylobacter fetus subsp. venerealis in preputial samples from bulls. | the diagnosis of bovine genital campylobacteriosis (bgc) presents significant challenges, as traditional methods lack sensitivity when prolonged transport of samples is required. assays of preputial samples by means of real-time polymerase chain reaction (pcr) provide good sensitivity and high throughput capabilities. however, there is limited information on the acceptable duration of transport and temperature during transport of samples. in addition, the use of pooled samples has proven to be a ... | 2016 | 26733730 |
| infectious reproductive disease pathogens in dairy herd bulls. | investigate the presence of infectious reproductive disease pathogens in dairy herd bulls in south-west victoria, australia, using a cross-sectional study. | 2015 | 26412115 |
| assessing the intra-species genetic variability in the clonal pathogen campylobacter fetus: crisprs are highly polymorphic dna markers. | campylobacter fetus is a gram-negative, microaerophilic bacterium that infects animals and humans. the subspecies campylobacter fetus subsp. fetus (cff) affects a broad range of vertebrate hosts and induces abortion in cows and sheep. campylobacter fetus subsp. venerealis (cfv) is restricted to cattle and causes the endemic disease bovine genital campylobacteriosis, which triggers reproductive problems and is responsible for major economic losses. campylobacter fetus subsp. testudinum (cft) has ... | 2017 | 27867047 |
| campylobacter-associated diseases in animals. | campylobacter includes a group of genetically diverse species causing a range of diseases in animals and humans. the bacterium is frequently associated with two economically important and epidemiologically distinct reproductive diseases in ruminants: enzootic infectious infertility in cattle owing to campylobacter fetus subsp. venerealis and abortions in sheep, goats, and cattle. septic abortion, usually epizootic in sheep, has been historically associated with c. fetus subsp. fetus and to a les ... | 2017 | 27860495 |
| a review of sexually transmitted bovine trichomoniasis and campylobacteriosis affecting cattle reproductive health. | the objective is to discuss sexually transmitted diseases caused by tritrichomonas foetus (t foetus) and campylobacter fetus (c fetus) subsp. venerealis, with a focus on prevalence, pathogenesis, and diagnosis in cows and bulls. diagnosis and control are problematic because these diseases cause severe reproductive losses in cows, but in bulls are clinically asymptomatic, which allows the disease to flourish, especially in the absence of legislated control programs. we review research regarding p ... | 2016 | 26679515 |
| infective internal iliac artery aneurysm caused by campylobacter fetus. | a 67-year-old man with a persistent high fever was diagnosed to have an infective aneurysm in his left internal iliac artery. a blood culture detected a gram-negative spiral rod that was first identified as campylobacter fetus subsp. venerealis based on a matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms) analysis. however, the strain was finally confirmed to be campylobacter fetus subsp. fetus based on a genetic analysis. the infection was successfully t ... | 2015 | 26278295 |
| [efficiency of bacteriological culture and the immunofluorescent assay to detect campylobacter fetus in bovine genital fluids]. | bovine genital campylobacteriosis is a reproductive disease that affects cattle production. it is caused by campylobacter fetus subspecies, c. fetus fetus (cff) and c. fetus venerealis (cfv). the aim of this study was to identify the presence of c. fetus in genital fluids by bacteriological culture and direct immunofluorescence (dif) and to compare the results. two groups of 6 heifers and 5 bulls, one infected with cff (cff group) and the other with cfv (cfv group) were formed. two heifers and 2 ... | 2015 | 26187267 |
| prevalence and risk factors associated with bovine genital campylobacteriosis and bovine trichomonosis in the state of pernambuco, brazil. | the aim of the present study was to determine the prevalence and risk factors associated with campylobacter fetus subsp. venerealis and tritrichomonas foetus infection in cows from dairy herds in the state of pernambuco, brazil. in total, 383 samples of cervico-vaginal mucus were collected from cows on 21 herds in 19 districts. genomic dna was extracted from the samples and submitted for polymerase chain reaction analysis. an investigative questionnaire was used to analyze the risk factors, usin ... | 2015 | 25633914 |
| evaluation and histological examination of a campylobacter fetus subsp. venerealis small animal infection model. | bovine genital campylobacteriosis (bgc), caused by campylobacter fetus subsp. venerealis, is associated with production losses in cattle worldwide. this study aimed to develop a reliable bgc guinea pig model to facilitate future studies of pathogenicity, abortion mechanisms and vaccine efficacy. seven groups of five pregnant guinea pigs (1 control per group) were inoculated with one of three strains via intra-peritoneal (ip) or intra-vaginal routes. samples were examined using culture, pcr and h ... | 2015 | 25599935 |
| campylobacter fetus subsp. venerealis adhesion to mdbk cells. | | 2014 | 25444136 |
| clinical sensitivity and specificity of a real-time pcr assay for campylobacter fetus subsp venerealis in preputial samples from bulls. | to determine clinical sensitivity and specificity of a quantitative real-time pcr (qrt-pcr) assay for campylobacter fetus subsp venerealis (cfv) in preputial samples of bulls. | 2014 | 25157889 |
| pregnancy rates of beef cattle are not affected by campylobacter fetus subsp. venerealis real-time pcr-positive breeding sires in new zealand. | campylobacter fetus subspecies venerealis (c. fetus venerealis) is the causal agent of bovine genital campylobacteriosis, a venereal disease that is asymptomatic in bulls but responsible for reproductive wastage in female cattle. in new zealand, a commercial real-time pcr assay was introduced in 2007 to identify the dna of this pathogen in preputial scrapings; however, concerns were raised about the specificity of the test following anecdotal reports of a high number of test-positive bulls with ... | 2014 | 24580097 |
| isolation rates of campylobacter fetus subsp venerealis from bovine preputial samples via passive filtration on nonselective medium versus selective medium, with and without transport medium. | to compare the recovery rates of campylobacter fetus subsp venerealis (cfv) from preputial scrapings of infected bulls with passive filtration on selective medium versus nonselective medium, with and without transport medium. | 2013 | 23879843 |
| real time pcr to detect and differentiate campylobacter fetus subspecies fetus and campylobacter fetus subspecies venerealis. | bovine venereal campylobacter infection, caused by campylobacter fetus venerealis, is of significant economic importance to the livestock industry. unfortunately, the successful detection and discrimination of c. fetus venerealis from c. fetus fetus continue to be a limitation throughout the world. there are several publications warning of the problem with biotyping methods as well as with recent molecular based assays. in this study, assessed on 1071 isolates, we report on the successful develo ... | 2013 | 23811208 |
| evaluation of molecular assays for identification campylobacter fetus species and subspecies and development of a c. fetus specific real-time pcr assay. | phenotypic differentiation between campylobacter fetus (c. fetus) subspecies fetus and c. fetus subspecies venerealis is hampered by poor reliability and reproducibility of biochemical assays. aflp (amplified fragment length polymorphism) and mlst (multilocus sequence typing) are the molecular standards for c. fetus subspecies identification, but these methods are laborious and expensive. several pcr assays for c. fetus subspecies identification have been described, but a reliable comparison of ... | 2013 | 23810970 |
| spatial and temporal epidemiology of bovine trichomoniasis and bovine genital campylobacteriosis in la pampa province (argentina). | the venereal diseases bovine trichomoniasis (bt) and bovine genital campylobacteriosis (bcg) cause economic losses in endemic areas like la pampa province in argentina, where beef cattle are usually managed extensively. this study used data compiled under a provincial programme for the control and eradication of bt and bgc (pce) to determine the spatio-temporal distribution of these diseases and identify spatial clusters. the study population comprised 29,178 non-virgin bulls drawn from 3766 her ... | 2013 | 23518247 |
| campylobacter fetus subspecies: comparative genomics and prediction of potential virulence targets. | the genus campylobacter contains pathogens causing a wide range of diseases, targeting both humans and animals. among them, the campylobacter fetus subspecies fetus and venerealis deserve special attention, as they are the etiological agents of human bacterial gastroenteritis and bovine genital campylobacteriosis, respectively. we compare the whole genomes of both subspecies to get insights into genomic architecture, phylogenetic relationships, genome conservation and core virulence factors. pan ... | 2012 | 22890137 |
| application of direct polymerase chain reaction assays for campylobacter fetus subsp. venerealis and tritrichomonas foetus to screen preputial samples from breeding bulls in cow-calf herds in western canada. | the primary objectives of this study were to estimate the prevalence of campylobacter fetus subsp. venerealis (cfv) and tritrichomonas foetus in breeding bulls from a sentinel cohort of cow-calf herds in western canada and to estimate the association between positive test status and non-pregnancy. the final objective was to evaluate the application of these tests when: i) screening bulls in the absence of a recognized problem with reproductive performance, and ii) testing for diagnosis of poor p ... | 2017 | 28408776 |