| phylogeny of thiobacillus cuprinus and other mixotrophic thiobacilli: proposal for thiomonas gen. nov. | the complete 5s and 16s ribosomal dna (rdna) sequences of the facultatively chemolithotrophic bacterium thiobacillus cuprinus and results of a comparison of these sequences with homologous sequences from several proteobacterial species supported affiliation of t. cuprinus with the beta 1 subgroup of the proteobacteria. t. cuprinus, thiobacillus intermedius, thiobacillus perometabolis, and thiobacillus thermosulfatus form a phylogenetic cluster that comprises some of the thiobacilli capable of mi ... | 1997 | 9103643 |
| genomic organization analysis of acidophilic chemolithotrophic bacteria using pulsed field gel electrophoretic techniques. | the genomic organization of acidophilic chemolithotrophic bacteria belonging to the genus thiobacillus, thiomonas and leptospirillum was studied using pulsed field gel electrophoresis techniques (pfge). the electrophoretic analysis of intact dna prepared from different strains showed that all have a circular chromosome, with sizes ranging from 1.9 mb for leptospirillum ferrooxidans atcc 49879, the smallest genome for an acidophilic strict chemolithoautotrophic microorganism, to 3.8 mb for thiomo ... | 1998 | 9893951 |
| an arsenic(iii)-oxidizing bacterial population: selection, characterization, and performance in reactors. | to select an autotrophic arsenic(iii)-oxidizing population, named caso1, and to evaluate the performance of the selected bacteria in reactors. | 2002 | 12234349 |
| bacterial immobilization and oxidation of arsenic in acid mine drainage (carnoulès creek, france). | the acid waters (ph=2.73-3.37) originating from the carnoulès mine tailings contain high dissolved concentrations of arsenic (1-3.5 mmol l(-1)) and iron (20-40 mmol l(-1)). at the outlet, arsenite predominates. during the first 30 m of downflow, 20-60% is removed by coprecipitation with fe(iii). this process results from bacterially mediated as- and fe-oxidation. the precipitation rates in the creek depend on the oxygen concentration in spring water and are lower during the dry summer period whe ... | 2003 | 12767295 |
| bacterial formation of tooeleite and mixed arsenic(iii) or arsenic(v)-iron(iii) gels in the carnoulès acid mine drainage, france. a xanes, xrd, and sem study. | the oxidation of fe(ii) in acid mine drainage (amd) leads to the precipitation of fe(iii) compounds which may incorporate toxic elements, such as arsenic (as), within their structure or adsorb them at their surface, thus limiting their mobility. the present work provides evidence for spatial and seasonal variations of microbial activity that influence arsenite oxidation and as immobilization in the heavily contaminated amd from the carnoulès mine, gard, france ([as iii] = 80 to 280 mg x l(-1) in ... | 2003 | 12775038 |
| mediation of arsenic oxidation by thiomonas sp. in acid-mine drainage (carnoulès, france). | to isolate, identify, and characterize heterotrophic bacteria in acid-mine drainage that mediate oxidation of as(iii). | 2003 | 12911697 |
| mediation of arsenic oxidation by thiomonas sp. in acid-mine drainage (carnoules, france). | | 2004 | 15078539 |
| accelerated biodegradation of cement by sulfur-oxidizing bacteria as a bioassay for evaluating immobilization of low-level radioactive waste. | disposal of low-level radioactive waste by immobilization in cement is being evaluated worldwide. the stability of cement in the environment may be impaired by sulfur-oxidizing bacteria that corrode the cement by producing sulfuric acid. since this process is so slow that it is not possible to perform studies of the degradation kinetics and to test cement mixtures with increased durability, procedures that accelerate the biodegradation are required. semicontinuous cultures of halothiobacillus ne ... | 2004 | 15466547 |
| isolation and characterization of sulphur-oxidizing thiomonas sp. and its potential application in biological deodorization. | to isolate and characterize a sulphur-oxidizing bacterial strain from activated sludge and to evaluate its potential application in biological deodorization. | 2004 | 15548301 |
| microbiology of a wetland ecosystem constructed to remediate mine drainage from a heavy metal mine. | a pilot passive treatment plant (pptp) was constructed to evaluate the potential of a composite wetland system to remediate acidic, metal-rich water draining the former wheal jane tin, in cornwall, england. the treatment plant consists of three separate and controllable composite systems, each of which comprises a series of aerobic wetlands for iron oxidation and precipitation, a compost bioreactor for removing chalcophilic metals and to generate alkalinity, and rock filter ponds for removing so ... | 2005 | 15680626 |
| biogeochemistry of the compost bioreactor components of a composite acid mine drainage passive remediation system. | the compost bioreactor ("anaerobic cell") components of three composite passive remediation systems constructed to treat acid mine drainage (amd) at the former wheal jane tin mine, cornwall, uk were studied over a period of 16 months. while there was some amelioration of the preprocessed amd in each of the three compost bioreactors, as evidenced by ph increase and decrease in metal concentrations, only one of the cells showed effective removal of the two dominant heavy metals (iron and zinc) pre ... | 2005 | 15680629 |
| oxidation of arsenite by thiomonas strains and characterization of thiomonas arsenivorans sp. nov. | a novel bacterium, strain b6(t) (t=type strain), was isolated from a disused mine site by growth using arsenite [as(iii)] as energy source in a simple mineral medium. cells of strain b6(t) were rod-shaped, gram-negative, non-sporulating and motile. optimum growth occurred at temperatures between 20 and 30 degrees c, and at ph between 4.0 and 7.5. strain b6(t) grew chemoautotrophically on as(iii), sulphur and thiosulphate, and also heterotrophically on yeast extract and a variety of defined organ ... | 2006 | 16341463 |
| confirmation of thiomonas delicata (formerly thiobacillus delicatus) as a distinct species of the genus thiomonas moreira and amils 1997 with comments on some species currently assigned to the genus. | the transfer of thiobacillus delicatus to the genus thiomonas as a distinct species, thiomonas delicata (type strain nbrc 14566t), is confirmed by its morphological and physiological properties, dna-dna hybridization and the grouping of its 16s rrna gene sequence with those of other species of the genus. an emended formal description of thiomonas delicata is given. the status of thiomonas cuprina dsm 5495t as a member of the genus is reconsidered. | 2006 | 17082389 |
| isolation of a carbon disulfide utilizing thiomonas sp. and its application in a biotrickling filter. | the carbon disulfide (cs2)-oxidizing bacterium thiomonas sp. wzw was enriched and isolated using activated sewage sludge as inoculum. growth of thiomonas sp. wzw was observed on cs2, thiosulfate, dimethylsulfide (dms), dimethyldisulfide (dmds), and h2s. no growth occurred on dimethylsulfoxide, methanol, acetate, and on complex media with glucose, yeast extract, or tryptone. dmds-grown cells respired cs2, dms, and dmds, while thiosulfate-grown cells did not respire cs2. chemostat cultures growing ... | 2007 | 17089121 |
| succession of sulfur-oxidizing bacteria in the microbial community on corroding concrete in sewer systems. | microbially induced concrete corrosion (micc) in sewer systems has been a serious problem for a long time. a better understanding of the succession of microbial community members responsible for the production of sulfuric acid is essential for the efficient control of micc. in this study, the succession of sulfur-oxidizing bacteria (sob) in the bacterial community on corroding concrete in a sewer system in situ was investigated over 1 year by culture-independent 16s rrna gene-based molecular tec ... | 2007 | 17142362 |
| biofilms of as(iii)-oxidising bacteria: formation and activity studies for bioremediation process development. | the formation and activity of an as(iii)-oxidising biofilm in a bioreactor, using pozzolana as bacterial growth support, was studied for the purpose of optimising fixed-bed bioreactors for bioremediation. after 60 days of continuous functioning with an as(iii)-contaminated effluent, the active biofilm was found to be located mainly near the inflow rather than homogeneously distributed. biofilm development by the caso1 bacterial consortium and by thiomonas arsenivorans was then studied both on po ... | 2007 | 17846760 |
| arsenite oxidation by a chemoautotrophic moderately acidophilic thiomonas sp.: from the strain isolation to the gene study. | an autotrophic bacterium able to gain energy from the oxidation of arsenite was isolated from arsenite-containing acid mine drainage waters. it belongs to the genus thiomonas as shown by dna-dna hybridization experiments, 16s rrna gene sequence, quinone and fatty acid content analyses. carboxysomes were observed and the cbbsl genes encoding the ribulose 1,5-bisphosphate carboxylase/oxygenase were detected, confirming that this bacterium is able to fix co(2). arsenite oxidation was catalysed by a ... | 2008 | 17894815 |
| reassessment of the phylogenetic relationships of thiomonas cuprina. | the published sequence of the 16s rrna gene of thiomonas cuprina strain hö5 (=dsm 5495t) (genbank accession no. u67162) was found to be erroneous. the 16s rrna genes from the type strain held by the dsmz since 1990 (dsm 5495t=nbrc 102145t) and strain hö5 maintained frozen in the universität regensburg for 23 years (=nbrc 102094) were sequenced and found to be identical, but to show no significant similarity to the u67162 sequence. this also casts some doubt on the previously published 5s and 23s ... | 2007 | 17978245 |
| ph gradient-induced heterogeneity of fe(iii)-reducing microorganisms in coal mining-associated lake sediments. | lakes formed because of coal mining are characterized by low ph and high concentrations of fe(ii) and sulfate. the anoxic sediment is often separated into an upper acidic zone (ph 3; zone i) with large amounts of reactive iron and a deeper slightly acidic zone (ph 5.5; zone iii) with smaller amounts of iron. in this study, the impact of ph on the fe(iii)-reducing activities in both of these sediment zones was investigated, and molecular analyses that elucidated the sediment microbial diversity w ... | 2008 | 18083864 |
| profiling of microbial communities in a bioreactor for treating hydrocarbon-sulfide-containing wastewater. | a technology of polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) was used to profile the structure and dynamic changes of microbial communities in a bioreactor for treating hydrocarbon-sulfide-containing (hsc) wastewater. the results showed that the heterotrophic genus of acinetobacter and the autotrophic genera of thiobacillus and thiomonas could survive well in all of three operating conditions. some special genera were also observed with changes of micro-ecoenviron ... | 2008 | 18817065 |
| carbon and arsenic metabolism in thiomonas strains: differences revealed diverse adaptation processes. | thiomonas strains are ubiquitous in arsenic-contaminated environments. differences between thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. for this purpose, five thiomonas strains, that are interesting in terms of arsenic metabolism were selected: t. arsenivorans, thiomonas spp. wj68 and 3as are able to oxidise as(iii), while thiomonas sp. ynys1 and t. perometabolis are not. moreover, t. arsenivorans and 3as present interesting physiologic ... | 2009 | 19549320 |
| analysis of bacteria communities in an up-flow fixed-bed (ufb) bioreactor for treating sulfide in hydrocarbon wastewater. | an up-flow fixed-bed (ufb) bioreactor with patented functional polyurethane foam (fpuf) carriers was used to treat sulfide in hydrocarbon wastewater. community compositions of autotrophic and heterotrophic bacteria were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). dgge results showed that a relatively stable bacterial community composed of heterotrophic and autotrophic bacteria formed in the bioreactor by the end of experiment, which ensured 92-100% s ... | 2009 | 19559604 |
| thiomonas bhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium. | a novel aerobic, obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium, s10t, was isolated from hot-spring sediment samples collected from atri, bhubaneswar, india. the cells of this isolate stained gram-negative and were strictly aerobic, non-sporulating, rod-shaped and motile with a single polar flagellum. strain s10t was positive for oxidase and catalase activities. it was capable of utilizing thiosulfate under mixotrophic growth conditions. mixotrophic growth was o ... | 2009 | 19605731 |
| thiomonas islandica sp. nov., a moderately thermophilic, hydrogen- and sulfur-oxidizing betaproteobacterium isolated from a hot spring. | a novel, hydrogen- and sulfur-oxidizing bacterium, designated strain 6c(t), was isolated from a hot spring in graendalur, south-western iceland. cells of this organism were gram-reaction-negative, rod-shaped and motile. the strain grew aerobically and was capable of chemolithotrophic growth on thiosulfate and hydrogen, heterotrophic growth on pyruvate, oxalate, acetate and on glutamate in the presence of yeast extract and mixotrophic growth on several organic compounds, thiosulfate and/or hydrog ... | 2011 | 20173005 |
| structure, function, and evolution of the thiomonas spp. genome. | bacteria of the thiomonas genus are ubiquitous in extreme environments, such as arsenic-rich acid mine drainage (amd). the genome of one of these strains, thiomonas sp. 3as, was sequenced, annotated, and examined, revealing specific adaptations allowing this bacterium to survive and grow in its highly toxic environment. in order to explore genomic diversity as well as genetic evolution in thiomonas spp., a comparative genomic hybridization (cgh) approach was used on eight different strains of th ... | 2010 | 20195515 |
| analysis of oral microbiota in children with dental caries by pcr-dgge and barcoded pyrosequencing. | oral microbiota plays a vital role in maintaining the homeostasis of oral cavity. dental caries are among the most common oral diseases in children and pathogenic bacteria contribute to the development of the disease. however, the overall structure of bacterial communities in the oral cavity from children with dental caries has not been explored deeply heretofore. we used high-throughput barcoded pyrosequencing and pcr-denaturing gradient gel electrophoresis (dgge) to examine bacterial diversity ... | 2010 | 20614117 |
| asiii oxidation by thiomonas arsenivorans in up-flow fixed-bed reactors coupled to as sequestration onto zero-valent iron-coated sand. | the combined processes of biological as(iii) oxidation and removal of as(iii) and as(v) by zero-valent iron were investigated with synthetic water containing high as(iii) concentration (10 mg l(-1)). two up-flow fixed-bed reactors (r1 and r2) were filled with 2 l of sieved sand (d = 3 ± 1 mm) while zero-valent iron powder (d = 76 μm; 1% (w/w) of sand) was mixed evenly with sand in r2. thiomonas arsenivorans was inoculated in the two reactors. the pilot unit was studied for 33 days, with hrt of 4 ... | 2010 | 20850864 |
| bacteria involved in sulfur amendment oxidation and acidification processes of alkaline 'alperujo' compost. | eight strains of sulfur oxidizing bacteria were isolated from alkaline 'alperujo' compost, seven being identified as paracoccus thiocyanatus and one as halothiobacillus neapolitanus. this was the first time that p. thiocyanatus was isolated from mature compost. acidification capability of isolated strains was compared with type strains h. neapolitanus cip104769, thiobacillus denitrificans cip104767 and thiomonas intermedia cip104401. indigenous p. thiocyanatus strains were as much as or more eff ... | 2010 | 20970324 |
| proposal that the arsenite-oxidizing organisms, thiomonas cuprina and 'thiomonas arsenivorans' be reclassified as strains of thiomonas delicata. | the three as(iii)-oxidizing betaproteobacteria thiomonas delicata, thiomonas cuprina and 'thiomonas arsenivorans' were isolated from mining sites in geographically distinct areas, namely japan, germany and france, respectively. they are all able to oxidize as(iii), but only 't. arsenivorans' and t. cuprina show efficient autotrophic growth with as(iii) and are able to grow on single carbon compounds. those two organisms are motile, but t. delicata is not. only t. cuprina can grow autotrophically ... | 2011 | 21216915 |
| characterization of the active bacterial community involved in natural attenuation processes in arsenic-rich creek sediments. | acid mine drainage of the carnoulès mine (france) is characterized by acid waters containing high concentrations of arsenic and iron. in the first 30 m along the reigous, a small creek draining the site, more than 38% of the dissolved arsenic was removed by co-precipitation with fe(iii), in agreement with previous studies, which suggest a role of microbial activities in the co-precipitation of as(iii) and as(v) with fe(iii) and sulfate. to investigate how this particular ecosystem functions, the ... | 2011 | 21318282 |
| role of exopolymeric substances (eps) in the stability of the biofilm of thiomonas arsenivorans grown on a porous mineral support. | biochemical methods were selected to evaluate the role of exopolymeric substances in the stability of biofilms used in bioremediation processes. biofilms of thiomonas arsenivorans formed on pozzolana were thus treated with pronase (protein target), lectins (con a or pna), calcofluor or periodic acid (polysaccharides target), dnase (dna target), and lipase (triglycerides target). neither protease nor dnase treatments had any effect on bacterial adhesion. lectins and calcofluor treatments mainly a ... | 2011 | 21364301 |
| characteristics of a phylogenetically ambiguous, arsenic-oxidizing thiomonas sp., thiomonas arsenitoxydans strain 3as(t) sp. nov. | a moderately acidophilic, facultative chemoautotrophic, as(iii)-oxidizing thiomonas sp. (strain 3as(t)) was previously shown, on the basis of comparative 16s rrna gene sequences, to be closely related to both tm. perometabolis dsm 18570(t) and tm. intermedia dsm 18155(t). while it had shared many physiological traits with tm. intermedia (t), a mean dna-dna hybridization value (ddhv) of 47.2% confirmed that strain 3as(t) was not a strain of tm. intermedia, though the situation with regard to tm. ... | 2011 | 21409355 |
| subinhibitory arsenite concentrations lead to population dispersal in thiomonas sp. | biofilms represent the most common microbial lifestyle, allowing the survival of microbial populations exposed to harsh environmental conditions. here, we show that the biofilm development of a bacterial species belonging to the thiomonas genus, frequently found in arsenic polluted sites and playing a key role in arsenic natural remediation, is markedly modified when exposed to subinhibitory doses of this toxic element. indeed, arsenite [as(iii)] exposure led to a considerable impact on biofilm ... | 2011 | 21876737 |
| effects of stimulation of copper bioleaching on microbial community in vineyard soil and copper mining waste. | long-term copper application in vineyards and copper mining activities cause heavy metal pollution sites. such sites need remediation to protect soil and water quality. bioremediation of contaminated areas through bioleaching can help to remove copper ions from the contaminated soils. thus, the aim of this work was to evaluate the effects of different treatments for copper bioleaching in two diverse copper-contaminated soils (a 40-year-old vineyard and a copper mining waste) and to evaluate the ... | 2011 | 21947860 |
| Performances of two biotrickling filters in treating H(2)S-containing waste gases and analysis of corresponding bacterial communities by pyrosequencing. | Two identical biotrickling filters named BTFa and BTFb were run in parallel to examine their performances in removing hydrogen sulfide. BTFa was filled with ceramic granules, and BTFb was filled with volcanic rocks. The results showed that BTFb was more robust than BTFa under acidic conditions. At empty bed residence times (EBRTs) of 20 and 15 s, the removal efficiency of BTFa was close to 100%. At EBRTs of 10 and 5 s, the removal efficiency of BTFa slightly decreased. The removal efficiencies o ... | 2011 | 22207217 |