| a beta-lactamase-overproducing strain of alcaligenes denitrificans subsp. xylosoxydans isolated from a case of meningitis. | a resistant (r) clinical isolate of alcaligenes denitrificans subsp. xylosoxydans was recovered from csf during treatment including piperacillin. this variant selected in vivo, and a second variant obtained in vitro from the initially susceptible (s) strain, both exhibited resistance to penicillins (ticarcillin, piperacillin) and cephalosporins, but remained susceptible to latamoxef and imipenem. clavulanate (2 mg/l) restored the susceptibility of the two r-variants to penicillins. a beta-lactam ... | 1992 | 1289351 |
| plasmid pcbi carries genes for anaerobic benzoate catabolism in alcaligenes xylosoxidans subsp. denitrificans pn-1. | pseudomonas sp. strain pn-1 is reclassified as alcaligenes xylosoxidans subsp. denitrificans pn-1. strain pn-1 is a gram-negative, rod-shaped organism, is motile by means of lateral flagella, is oxidase positive, and does not ferment sugars. plasmid pcbi, carrying genes for the anaerobic degradation of benzoate in strain pn-1, is 17.4 kilobase pairs in length and is transmissible to a number of denitrifying pseudomonas aeruginosa and pseudomonas stutzeri strains. a restriction endonuclease map w ... | 1987 | 2822651 |
| degradation of mono- and dichlorobenzoic acid isomers by two natural isolates of alcaligenes denitrificans. | | 1990 | 2403263 |
| susceptibility of alcaligenes denitrificans subspecies xylosoxydans to beta-lactam antibiotics. | the susceptibility of 56 clinical isolates and two reference strains of alcaligenes denitrificans subsp. xylosoxydans to beta-lactam agents was tested and related to beta-lactamase activity of the strains. the mics of 12 beta-lactams determined by an agar dilution method showed that all the strains were sensitive to imipenem and moxalactam. forty-one cloxacillin-sensitive beta-lactamase producing strains were highly susceptible to azlocillin, piperacillin and ticarcillin, and less susceptible to ... | 1990 | 2387293 |
| two resistance phenotypes to beta-lactams of alcaligenes denitrificans subsp. xylosoxydans in relation to beta-lactamase types. | | 1990 | 2351630 |
| immunological properties of a penicillinase produced by alcaligenes denitrificans subsp. xylosoxydans. | rabbit antiserum was prepared to the penicillinase of alcaligenes denitrificans subsp. xylosoxydans gn14062. the antibody gave a single precipitin line in double immunodiffusion assays with the penicillinases from strain gn14062 and from two other a. denitrificans subsp. xylosoxydans strains. the precipitin line given by the gn14062 enzyme gave a reaction of complete identity with the precipitin lines of the penicillinases from the other a. denitrificans subsp. xylosoxydans strains. furthermore, ... | 1987 | 3119550 |
| isolation and sequencing of the alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from pseudomonas aeruginosa and alcaligenes faecalis. | the gene (azu) encoding azurin from alcaligenes denitrificans has been cloned and sequenced. the gene codes for a pre-protein with a 19-aa signal peptide. comparison with the sequences coding for the blue copper proteins from pseudomonas aeruginosa and alcaligenes faecalis reveals the presence of ntra and fnr boxes in front of all three genes, instead of a regular [-10, -35]-promoter. in p. aeruginosa, the azu gene is terminated by a bidirectional terminator and flanked by open reading frames on ... | 1990 | 2116366 |
| the in-vitro susceptibility of alcaligenes denitrificans subsp. xylosoxidans to 40 antimicrobial agents. | | 1990 | 2081727 |
| [a clinical evaluation of sultamicillin fine granules in the treatment of meibomianitis]. | this study was performed to evaluate the clinical efficacy and the safety of sultamicillin (sbtpc) fine granules in the treatment of patients with meibomianitis. a dose of 375 mg sbtpc granules was orally given to 10 patients with meibomianitis after each meal, three times a day. the results obtained are summarized as follows. efficacies were rated as good in 7 cases, fair in 2 and poor in 1 with an efficacy ratio of 70.0%. no side effects were observed throughout the study. the organisms isolat ... | 1988 | 3249376 |
| resonance raman spectra of plastocyanin and pseudoazurin: evidence for conserved cysteine ligand conformations in cupredoxins (blue copper proteins). | new resonance raman (rr) spectra at 15 k are reported for poplar (populus nigra) and oleander (oleander nerium) plastocyanins and for alcaligenes faecalis pseudoazurin. the spectra are compared with those of other blue copper proteins (cupredoxins). in all cases, nine or more vibrational modes between 330 and 460 cm-1 can be assigned to a coupling of the cu-s(cys) stretch with cys ligand deformations. the fact that these vibrations occur at a relatively constant set of frequencies is testimony t ... | 1991 | 1932014 |
| novel cyanide-hydrolyzing enzyme from alcaligenes xylosoxidans subsp. denitrificans. | a cyanide-metabolizing bacterium, strain df3, isolated from soil was identified as alcaligenes xylosoxidans subsp. denitrificans. whole cells and cell extracts of strain df3 catalyzed hydrolysis of cyanide to formate and ammonia (hcn + 2h2o----hcooh + nh3) without forming formamide as a free intermediate. the cyanide-hydrolyzing activity was inducibly produced in cells during growth in cyanide-containing media. cyanate (ocn-) and a wide range of aliphatic and aromatic nitriles were not hydrolyze ... | 1991 | 1872607 |
| microbial degradation of shrimp-shell waste. | a total of 40 strains of bacteria were isolated and tested for their potentiality to degrade chitin and utilize shrimp-shell waste for the production of chitinase. the activity ratio, percentage of weight loss and enzyme activity were determined for cultures exhibiting highest chitinolytic activities. the most active organisms were identified as alcaligenes denitrificans, bacillus amyloliquefaciens, b. megaterium and b. subtilis. the potentiality of the first two organisms to degrade chitin was ... | 1992 | 1512701 |
| five cases of alcaligenes pseudobacteraemia. | a cluster of five cases of pseudobacteraemia due to the organism alcaligenes denitrificans occurred in three hospital medical wards over a four week period. the same organism was isolated from four of twelve commercially prepared bottles for erythrocyte sedimentation tests. the most likely explanation for the outbreak is that the esr bottles were filled prior to inoculation of blood culture bottles. the outbreak was brought to an end by advising on correct procedure. | 1992 | 1481307 |
| a strictly anaerobic nitrate-reducing bacterium growing with resorcinol and other aromatic compounds. | with resorcinol as sole source of energy and organic carbon, two stains of gram-negative, nitrate-reducing bacteria were isolated under strictly anaerobic conditions. strain lubres1 was facultatively anaerobic and catalase- and superoxide dismutase-positive. this strain was affiliated with alcaligenes denitrificans on the basis of substrate utilization spectrum and peritrichous flagellation. strain lufres1 could grow only under anaerobic conditions with oxidized nitrogen compounds as electron ac ... | 1992 | 1444713 |
| identification of xenobiotic-degrading isolates from the beta subclass of the proteobacteria by a polyphasic approach including 16s rrna partial sequencing. | nineteen gram-negative, aerobic, biodegradative isolates were identified by using a polyphasic taxonomic approach. the presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone q-8) allowed allocation of these organisms to the beta subclass of the proteobacteria. on the basis of the results of additional characterization experiments (i.e., api 20ne tests, determinations of soluble protein patterns, and dna-dn ... | 1992 | 1371062 |
| preferential uptake of d-alpha-aminoadipate from a racemic mixture by an alcaligenes denitrificans. | | 1971 | 5581277 |
| characterization of d-aminoacylase from alcaligenes denitrificans da181. | the d-aminoacylase produced by alcaligenes denitrificans da181 was a new type of aminoacylase which had both high stereospecificity and specific activity. the molecular weight and isoelectric point of this enzyme were 58,000 and 4.4, respectively. the apparent km and kcat values of this enzyme for n-acetyl-d-methionine were estimated to be 0.48 mm and 6.24 x 10(4) min-1, respectively. the optimum temperature was 45 degrees c. the enzyme was stable up to 55 degrees c for 1 hr in the presence of 0 ... | 1992 | 1368943 |
| [comparative susceptibility of ochrobactrum anthropi, agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes denitrificans subsp. denitrificans, alcaligenes denitrificans subsp. xylosidans and bordetella bronchiseptica against 35 antibiotics including 17 beta-lactams]. | ochrobactrum anthropi, formerly known as "achromobacter sp." or cdc group vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). o. anthropi is a gram negative, motile, strictly aerobic, oxydase positive and non-fermentative bacteria with a strong urease activity. the susceptibility of 13 strains of o. anthropi was determined by agar diffusion method and compared to those of type strains of agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes ... | 1995 | 7567111 |
| degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures. | bacterial mixed cultures able to degrade the polycyclic aromatic hydrocarbons (pah) phenanthrene, fluorene and fluoranthene, were obtained from soil using conventional enrichment techniques. from these mixed cultures three pure strains were isolated: pseudomonas paucimobilis degrading phenanthrene; p. vesicularis degrading fluorene and alcaligenes denitrificans degrading fluoranthene. the maximum rates of pah degradation ranged from 1.0 mg phenanthrene/ml per day to 0.3 mg fluoranthene/ml per da ... | 1990 | 1366395 |
| the importance of asn47 for structure and reactivity of azurin from alcaligenes denitrificans as studied by site-directed mutagenesis and spectroscopy. | to study the importance of a rigid copper site for the structure and function of azurin, a mutant with a reduced number of internal hydrogen bonds around the copper has been prepared and characterized. to this purpose, the previously cloned azu gene from alcaligenes denitrificans (hoitink, c. w. g., woudt, l. p., turenhout, j. c. m., van de kamp, m., and canters, g. w. (1990) gene (amst.) 90, 15-20) was expressed in escherichia coli and an isolation and purification procedure for the azurin was ... | 1992 | 1321127 |
| physiological basis for preferential uptake of d-alpha-aminoadipate over the l-isomer by alcaligenes denitrificans. | alcaligenes denitrificans, pre-incubated with d-alpha-aminoadipate and assayed for l-isomer uptake without removal of extracellular d-isomer, exhibits a reduced rate of uptake and a reduced level at which steady state is achieved. during d- or l-isomer uptake, intracellular alpha-aminoadipate is exclusively the l-configuration. these data are consistent with an intracellular, mediated reduction in l-isomer uptake as the physiological basis for preferential d-alpha-aminoadipate uptake by a. denit ... | 1975 | 1138928 |
| carbon substrate utilization studies of some cultures of alcaligenes denitrificans, alcaligenes faecalis, and alcaligenes odorans isolated from clinical specimens. | one hundred and sixty-two cultures of alcaligenes species (a. denitrificans, a. faecalis, and a. odorans) of clinical origin were characterized by routine diagnostic and carbon substrate utilization techniques. the microorganisms were tested for their ability to utilize a total of 188 substrates. substrate utilization was assayed by (i) growth stimulation and (ii) substrate alkalinization. the a. denitrificans and a. odorans cultures had unique substrate utilization profiles for each species. th ... | 1978 | 701468 |
| transfer and expression of pcb-degradative genes into heavy metal resistant alcaligenes eutrophus strains. | sites polluted with organic compounds frequently contain inorganic pollutants such as heavy metals. the latter might inhibit the biodegradation of the organics and impair bioremediation. chromosomally located polychlorinated biphenyl (pcb) catabolic genes of alcaligenes eutrophus a5, achromobacter sp. lbs1c1 and alcaligenes denitrificans jb1 were introduced into the heavy metal resistant alcaligenes eutrophus strain ch34 and related strains by means of natural conjugation. mobile elements contai ... | 1994 | 7765842 |
| microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products. | microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ... | 1977 | 596877 |
| in vitro activity of fk-037, a novel parenteral cephalosporin, against bacterial isolates from neutropenic cancer patients. | the in vitro activity of fk-037, a novel parenteral cephalosporin, was compared to that of ceftazidime, aztreonam and piperacillin (agents often used in empiric regimens in cancer patients) against recent bacterial isolates from patients with cancer. fk-037 was either equal to or 2 to 16-fold more active than the comparative agents against members of the enterobacteriaceae. it was also active against acinetobacter spp., aeromonas spp., pseudomonas aeruginosa, and other pseudomonas spp. xanthomon ... | 1994 | 7813504 |
| purification and preliminary crystallographic studies on azurin and cytochrome c' from alcaligenes denitrificans and alcaligenes sp. ncib 11015. | | 1979 | 231110 |
| three-dimensional model of stellacyanin and its implications for electron transfer reactivity. | experimental data were combined with computational methods in constructing a hypothetical three-dimensional model for the blue single copper protein rhus stellacyanin (st). the known sequence of stellacyanin and its homology with plastocyanin (pc) were used together with the results of spectroscopic studies of the protein that yielded the current assignment of two histidines, one cysteine and a disulfide sulfur as copper ligands in stellacyanin. by computer graphics and energy minimization the f ... | 1988 | 2975707 |
| in-vitro activity of newer quinolones against aerobic bacteria. | nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ... | 1986 | 2940214 |
| 1h nuclear magnetic resonance study of the protonation behaviour of the histidine residues and the electron self-exchange reaction of azurin from alcaligenes denitrificans. | the proton nuclear magnetic resonance spectrum of azurin from alcaligenes denitrificans at ph 6.0 and 309 k is reported. proton signals from all methionine and histidine residues (among them the copper ligands) have been assigned. the data have been used to study the ph behaviour of his35 and to establish the electron self-exchange rate of the protein. his35 appears to be protonated at ph less than 4.5, possibly after rupture of a salt bridge. no effects of this protonation on the tertiary struc ... | 1988 | 2837576 |
| epr of azurins from pseudomonas aeruginosa and alcaligenes denitrificans demonstrates ph-dependence of the copper-site geometry in pseudomonas aeruginosa protein. | the x- and q-band epr spectra of pseudomonas aeruginosa (63cu)azurin and alcaligenes denitrificans azurin have been measured at ph = 5.2 and 9.2, in the presence and absence of 40% glycerol. the epr spectra of both proteins could properly be simulated by taking into account a spread in the tetrahedral angle of the copper site. the change in the epr spectrum of pseudomonas aeruginosa (63cu)azurin that is observed upon an increase of the ph from 5.2 to 9.2 is consistent with a small decrease of th ... | 1987 | 2828541 |
| spectrochemical studies on the blue copper protein azurin from alcaligenes denitrificans. | spectroscopic and electrochemical studies, incorporating electronic spectra, electron paramagnetic resonance (epr) spectra, resonance raman (rr) spectra, and measurements of the redox potential, have been carried out on the blue copper protein azurin, from alcaligenes denitrificans. these data are correlated with the refined crystal structure of this azurin and with corresponding data for other blue copper proteins. the electronic spectrum, characterized by an intense (epsilon = 5100 m-1 cm-1) c ... | 1987 | 3030404 |
| internal motion and electron transfer in proteins: a picosecond fluorescence study of three homologous azurins. | we have carried out a picosecond fluorescence study of holo- and apoazurins of pseudomonas aeruginosa (azurin pae), alcaligenes faecilis (azurin afe), and alcaligenes denitrificans (azurin ade). azurin pae contains a single, buried tryptophyl residue; azurin afe, a single surface tryptophyl residue; and azurin ade, tryptophyl residues in both environments. from anisotropy measurements we conclude that the interiors of azurins pae and ade are not mobile enough to enable motion of the indole ring ... | 1987 | 3111523 |
| in vitro susceptibility of alcaligenes denitrificans subsp. xylosoxidans to 24 antimicrobial agents. | the in vitro susceptibilities of 37 clinical isolates of alcaligenes denitrificans subsp. xylosoxidans to 24 antimicrobial agents were determined. imipenem was the only drug with consistent activity (mic for 90% of isolates, 2 micrograms/ml). piperacillin, ticarcillin-clavulanic acid, ceftazidime, and co-trimoxazole were active against most strains. all the isolates were resistant to ampicillin, cefazolin, cefuroxime, cefamandole, cefotetan, ceftriaxone, cefotaxime, aztreonam, amdinocillin, and ... | 1988 | 3163242 |
| selection of trichloroethene (tce) degrading bacteria that resist inactivation by tce. | two isoprene (2-methyl-1,3-butadiene) utilizing bacteria, alcaligenes denitrificans ssp. xylosoxidans je 75 and rhodococcus erythropolis je 77, were identified as highly efficient cooxidizers of tce, cis- and trans-dichloroethene, 1,1-dichloroethene and vinyl-chloride. isoprene grown cells eliminate chloride from tce in stoichiometric amounts and tolerate high concentrations of tce. | 1990 | 2244792 |
| photophysics of metalloazurins. | the fluorescence lifetimes of cu(ii), cu(i), ag(i), hg(ii), co(ii), and ni(ii) azurin pae from pseudomonas aeruginosa and cu(ii), cu(i), and hg(ii) azurin afe from alcaligenes faecalis were measured at 295 k by time-correlated single-photon counting. in addition, fluorescence lifetimes of cu(ii) azurin pae were measured between 30 and 160 k and showed little change in value. ultraviolet absorption difference spectra between metalloazurin pae and apoazurin pae were measured, as were the fluoresce ... | 1990 | 2119804 |
| structure of azurin from alcaligenes denitrificans refinement at 1.8 a resolution and comparison of the two crystallographically independent molecules. | the structure of the blue copper protein azurin, from alcaligenes denitrificans, has been refined crystallographically by restrained least-squares methods. the final crystallographic r value for 21,980 observed reflections to 1.8 a (1 a = 0.1 nm) resolution is 0.157. the asymmetric unit of the crystal contains two independent azurin molecules, the model for which comprises 1973 protein atoms, together with three so2-4 ions, and 281 water molecules. comparison of the two molecules shows very high ... | 1988 | 3210236 |
| hydrogen bonding of sulfur ligands in blue copper and iron-sulfur proteins: detection by resonance raman spectroscopy. | the resonance raman spectrum of the blue copper protein azurin from alcaligenes denitrificans exhibits nine vibrational modes between 330 and 460 cm-1, seven of which shift 0.4-3.0 cm-1 to lower energy after incubation of the protein in d2o. these deuterium-dependent shifts have been previously ascribed to exchangeable protons on imidazole ligands [nestor, l., larrabee, j. a., woolery, g., reinhammar, b., & spiro, t. g. (1984) biochemistry 23, 1084] or to exchangeable protons on amide groups whi ... | 1987 | 3442645 |
| purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced by bordetella pertussis and bordetella bronchiseptica. | hydroxamate siderophores of virulent bordetella pertussis and bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(s),11,18(s)- tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone+ ++, which was previously isolated from the taxonomically-related bacterial species alcaligenes denitrificans subsp. xylosoxydans. alcal ... | 1996 | 8744901 |
| in vitro activity of imipenem towards gram-negative bacilli. | the authors studied the in vitro antimicrobial activity of imipenem towards 355 gram-negative bacterial strains, taking into particular consideration unusual or dangerous species. the study was carried out on a comparative basis with piperacillin, cefotaxime, ceftazidime and gentamicin. ninety percent of the fermentative gram-negative strains were inhibited at concentrations less than or equal to 2 mg/l. imipenem inhibited 100% of strains of alcaligenes faecalis, alcaligenes denitrificans, flavo ... | 1986 | 3466723 |
| x-ray crystal structure of the two site-specific mutants his35gln and his35leu of azurin from pseudomonas aeruginosa. | the three-dimensional structures of two site-specific mutants of the blue copper protein azurin from pseudomonas aeruginosa have been solved by a combination of isomorphous replacement and patterson search techniques, and refined by energy-restrained least-squares methods. the mutations introduced by recombinant dna techniques involve residue his35, which was exchanged for glutamine and leucine, to probe for its suggested role in electron transfer. the two mutants, his35gln (h35q) and his35leu ( ... | 1991 | 1901363 |
| reductive dechlorination of 2,4-dichlorobenzoate to 4-chlorobenzoate and hydrolytic dehalogenation of 4-chloro-, 4-bromo-, and 4-iodobenzoate by alcaligenes denitrificans ntb-1. | alcaligenes denitrificans ntb-1, previously isolated on 4-chlorobenzoate, also utilized 4-bromo-, 4-iodo-, and 2,4-dichlorobenzoate but not 4-fluorobenzoate as a sole carbon and energy source. during growth, stoichiometric amounts of halide were released. experiments with whole cells and cell extracts revealed that 4-bromo- and 4-iodobenzoate were metabolized like 4-chlorobenzoate, involving an initial hydrolytic dehalogenation yielding 4-hydroxybenzoate, which in turn was hydroxylated to 3,4-di ... | 1987 | 3579283 |
| peritonitis caused by alcaligenes denitrificans subsp. xylosoxydans: case report and review of the literature. | we report the third human case of peritonitis caused by alcaligenes denitrificans subsp. xylosoxydans and review the english literature regarding community-acquired and nonsocomial infection and colonization that results from this bacterium. the biochemical and genetic characteristics supporting the pathogenic potential of a. denitrificans subsp. xylosoxydans are reviewed, and the antimicrobial susceptibility profile of the organism is summarized. | 1986 | 3771775 |
| [amino acid metabolism by thin layer chromatography for the differentiation of bordetella and alcaligenes species]. | the amino acid metabolism of bordetella and alcaligenes strains was investigated for differentiation purposes. all species investigated reacted differently with a number of amino acids. the genus bordetella could be differentiated from that of alcaligenes by its inability to metabolize l-lysine, l-arginine and d-histidine. the recently described species bordetella avium, as well as achromobacter xylosoxidans (yabuuchi), considered as alcaligenes denitrificans subsp. xylosoxidans, presented thems ... | 1986 | 3788350 |
| [nitrate, nitrite and nitrous oxide reduction by alcaligenes denitrificans and alcaligenes odorans]. | | 1973 | 4792486 |
| structure of azurin from alcaligenes denitrificans at 2.5 a resolution. | the structure of the blue copper protein, azurin, from alcaligenes denitrificans has been determined from an electron density map at a nominal resolution of 3.0 a. four isomorphous heavy-atom derivatives, prepared with kau(cn)2, uranyl acetate, hg(nh3)2cl2 and kau(cn)2 + uranyl acetate (a double derivative) were used to calculate phases by the method of isomorphous replacement. the overall figure of merit was 0.61. the two molecules in the asymmetric unit are related by an approximate 2-fold axi ... | 1983 | 6842609 |
| [identification of pseudomonas stutzeri with the nefermtest, neferm test 24 and api 20ne commercial test kits]. | a total of 35 strains of pseudomonas stutzeri were tested by commercial diagnostic kits nefermtest, nefermtest 24 (both lachema co., brno, czech republic) and api 20ne (biomérieus sa, rcs lyon, france). the best identification efficacy was achieved by means of the commercial api 20ne (97.1%). only 60% strains were correctly identified by means of nefermtest. nefermtest 24 improved successful identification (80%). most misidentified strains were identified as alcaligenes denitrificans and al. xyl ... | 1998 | 9611890 |
| evidence for two distinct azurins in alcaligenes xylosoxidans (ncimb 11015): potential electron donors to nitrite reductase. | we have isolated two type 1 copper-containing proteins (m(r) approximately 13k) from alcaligenes xylosoxidans (ncimb 11015) grown under denitrifying conditions. amino acid sequence analysis of these two proteins shows one to be the previously identified azurin (ambler, 1971), which we shall call azurin i, and the other to be a related, but previously undescribed, blue copper protein which we show to also be an azurin and propose to call azurin ii. thus, ncimb 11015 becomes the second system wher ... | 1995 | 7640272 |
| structure of azurin from achromobacter xylosoxidans ncib11015 at 2.5 a resolution. | the crystal structure of azurin from a denitrifying bacterium, achromobacter xylosoxidans ncib11015, has been refined at 2.5 a resolution using diffraction data obtained by means of synchrotron radiation at kek. crystals suitable for x-ray experiment were obtained by the macro-seeding method and an intensity data were obtained on imaging plates mounted on a weissenberg camera (rmerge = 0.09). the initial model was obtained by the molecular replacement method using the structure of azurin from al ... | 1994 | 7706206 |
| a gene from alcaligenes denitrificans that confers albicidin resistance by reversible antibiotic binding. | albicidin antibiotics specifically block prokaryote dna replication. the albicidin resistance gene (albb) cloned from a soil isolate of alcaligenes denitrificans encodes a 23 kda protein capable of detoxifying albicidin by reversible binding. this mechanism operates intracellularly to protect dna replication in albicidin-sensitive escherichia coli expressing the cloned resistance gene, which can be induced fivefold in the presence of 1.5 micrograms albicidin ml-1 in the surrounding medium. the c ... | 1995 | 7711894 |
| capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. | we used capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) analysis of pcr-amplified 16s rrna gene fragments for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli isolated from patients with cystic fibrosis (cf). target sequences were amplified by using forward and reverse primers labeled with various fluorescent dyes. the labeled pcr products were denatured by heating and separated by capillary gel electrophoresis with an autom ... | 1999 | 10488211 |
| purification and characterization of l-aminoacylase from alcaligenes denitrificans da181. | the l-aminoacylase produced intracellularly by alcaligenes denitrificans da181 was purified to homogeneity. this enzyme had an apparent molecular weight of 80,000, and was composed of two subunits of identical molecular weight. its isoelectric point was ph 5.1. the optimal reaction temperature and ph were 65 degrees c and 8.0, respectively. this enzyme showed specificity toward n-acetyl-derivative of hydrophobic l-amino acids with n-acetyl-l-valine as the favored substrate, followed by n-acetyl- ... | 1994 | 7764515 |
| identification of a carbenicillin-hydrolyzing beta-lactamase in alcaligenes denitrificans subsp. xylosoxydans. | eleven strains of alcaligenes denitrificans subsp. xylosoxydans produced a beta-lactamase with a pi of 5.7 with kinetic data characteristic of a pse-1-type enzyme. a carb-type enzyme was identified by using an intragenic dna probe of blacarb. hybridization of genomic dna after xbai restriction and pulsed-field electrophoresis suggested a chromosomal location for the gene. | 1995 | 7793891 |
| investigation of hospital-acquired infections due to alcaligenes denitrificans subsp. xylosoxydans by dna restriction fragment length polymorphism. | we demonstrate that dna restriction fragment length polymorphism determined by pulsed-field gel electrophoresis is very useful in the investigation of the epidemiology of hospital-acquired infections caused by alcaligenes denitrificans subsp. xylosoxydans. this approach showed that hospital-acquired infections caused by this opportunistic pathogen over a 6-month period in 10 patients hospitalized in an intensive care unit and a surgical unit were not a true outbreak. in addition, this molecular ... | 1994 | 7913093 |
| in vitro and in situ survivals of bacterial populations added to fresh water environments. | the fate of aeromonas hydrophila, alcaligenes denitrificans, vibrio cholerae non-01, pseudomonas putida and four different isolates of escherichia coli in fresh river water were assessed by using different microcosms (i.e., membrane diffusion chamber and erlenmeyer flask). when water samples were incubated at 16 +/- 1 degrees c, the differences in extent of survival among test bacteria were in general not significant. if the incubation temperature was raised to 29 +/- 1 degrees c, in the in situ ... | 1993 | 7982366 |
| 1h and 15n nuclear magnetic resonance assignments, secondary structure in solution, and solvent exchange properties of azurin from alcaligenes denitrificans. | complete sequential 1h and 15n resonance assignments for the reduced cu(i) form of the blue copper protein azurin (m(r) = 14,000, 129 residues) from alcaligenes denitrificans have been obtained at ph 5.5 and 32 degrees c using homo- and heteronuclear two-dimensional and heteronuclear three-dimensional nmr spectroscopy. comparison of the resonance assignments for the backbone protons with those of pseudomonas aeruginosa azurin, which is 68% homologous in its amino acid sequence and has a very sim ... | 1994 | 8142353 |
| distinctive electrophoretic pattern of esterases produced by alcaligenes species. | the esterases produced by 34 strains of alcaligenes faecalis, 16 strains of a. denitrificans subsp. xylosoxydans, 5 strains of a. piechaudii and 10 strains of a. denitrificans subsp. denitrificans were analysed by horizontal polyacrylamide-agarose gel electrophoresis. these enzymes were distinguished by their spectra of hydrolytic activity towards 5 synthetic substrates (hydrolytic type) and their electrophoretic mobilities (electrophoretic type). four hydrolytic types of esterases were produced ... | 1993 | 8210679 |
| unsuccessful treatment of capd peritonitis caused by alcaligenes xylosoxidans subsp. denitrificans. | | 1995 | 8570874 |
| structure of metal site in azurin, met121 mutants of azurin, and stellacyanin investigated by 111mcd perturbed angular correlation (pac). | the geometries of the metal sites in cadmium-substituted azurins have been investigated by 111mcd perturbed angular correlation (pac). the study includes wild type azurin as well as met121 mutants of azurin, where methionine has been substituted by ala, asn, asp, gln, glu, and leu. the nuclear quadrupole interaction of wild type azurin analyzed in the angular overlap model is well described as coordination of his46, his117, and cys112 and cannot be described by coordination of met121 and/or gly4 ... | 1995 | 7822282 |
| identification of alcaligin as the siderophore produced by bordetella pertussis and b. bronchiseptica. | the siderophores produced by iron-starved bordetella pertussis and b. bronchiseptica were purified and were found to be identical. using mass spectrometry and proton nuclear magnetic resonance, we determined that the siderophore produced by these organisms was identical to alcaligin, a siderophore produced by alcaligenes denitrificans. | 1995 | 7860593 |
| investigation of hospital-acquired infections due to alcaligenes denitrificans subsp. xylosoxidans by dna restriction fragment length polymorphism. | | 1995 | 8576385 |
| design of metal ion binding peptides. | two cyclic and branched peptides (pla and azu) were synthesized with the aim of reproducing the active site of the blue copper proteins plastocyanin and azurin. both peptides, designed on the basis of the x-ray structures of poplar plastocyanin and alcaligenes denitrificans azurin, contain the same coordinating residues of the parent native proteins. the visible spectra of pla in the presence of equimolar amount of cu(ii) strongly support the interaction between the peptide and copper(ii) ion. t ... | 1995 | 8589245 |
| paramagnetic cobalt and nickel derivatives of alcaligenes denitrificans azurin and its m121q mutant. a 1h nmr study. | using cobalt or nickel to replace copper in native azurin allows one to fingerprint the metal coordination site of the protein. the metal sites of wild type alcaligenes denitrificans azurin and its m121q mutant are clearly distinguishable through the paramagnetic 1h nmr spectra of the ni(ii) and co(ii) derivatives. in the wild type azurin, gly45 coordinates to nickel or cobalt, while met121 appears as a weak metal ligand. on the contrary, in the m121q azurin mutant, the metal exhibits a clear pr ... | 1996 | 8639662 |
| high-resolution crystal structures of two polymorphs of cytochrome c' from the purple phototrophic bacterium rhodobacter capsulatus. | the structures of two polymorphs of cytochrome c' from rhodobacter capsulatus (rccp) strain m110 have been determined by the molecular replacement method. iron anomalous scattering data were used to confirm the molecular replacement solution. the structures were refined at 1.72 angstrom and 2.0 angstrom resolution to r-values of 15.0% and 16.3%, respectively. the rccp molecule is a dimer and each of the identical 129 residue subunits folds as a four-helical bundle with a covalently bound heme gr ... | 1996 | 8676382 |
| [alcaligenes denitrificans subsp. xylosoxidans infection in a patient with corticodependent chronic respiratory insufficiency]. | | 1996 | 8685175 |
| keratitis caused by alcaligenes denitrificans subsp. xylosoxidans. | | 1996 | 8776574 |
| the mutation met121-->his creates a type-1.5 copper site in alcaligenes denitrificans azurin. | the cu ligand met121 in azurin of alcaligenes denitrificans was mutated to his. the spectroscopic and mechanistic properties of [m121h]azurin appear to be ph dependent with a pka of 3.8 due to the ionization of his121. the [m121h]azurin mutant exhibits two major distinct metal-site-coordination geometries which coexist in solution according to ph-dependent equilibrium. both species have been spectroscopically characterized by ultraviolet-visible, epr and resonance raman spectroscopies. at neutra ... | 1996 | 8841397 |
| bordetella trematum sp. nov., isolated from wounds and ear infections in humans, and reassessment of alcaligenes denitrificans rüger and tan 1983. | ten strains recognized on the basis of a computer-assisted numerical comparison of whole-cell protein patterns as members of a novel species belonging to the family alcaligenaceae were examined by using an integrated phenotypic and genotypic approach. this species, for which we propose the name bordetella trematum sp. nov., was more closely related to the type species of the genus bordetella (bordetella pertussis) than to the type species of the genus alcaligenes (alcaligenes faecalis) and had t ... | 1996 | 8863408 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22 | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9042758 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22. | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9133325 |
| coordination geometries for monovalent and divalent metal ions in [his121]azurin--studies using perturbed angular correlations of gamma-rays from 111ag and 111mcd. | the structural details of the metal site in the [his121]azurin mutant from alcaligenes denitrificans where the axial methionine has been replaced by a histidine have been studied after substitution with the divalent cadmium ion and the monovalent silver ion. the studies have been carried out in solution using the technique of perturbed angular correlations of gamma-rays (pac) of the two isotopes, 111ag and 111mcd. in the ph range 6-9, the pac spectra for cadmium-substituted [his121]azurin reveal ... | 1997 | 9428671 |
| rack-induced metal binding vs. flexibility: met121his azurin crystal structures at different ph. | the rack-induced bonding mechanism of metals to proteins is a useful concept for explaining the generation of metal sites in electron transfer proteins, such as the blue copper proteins, that are designed for rapid electron transfer. the trigonal pyramidal structure imposed by the protein with three strong equatorial ligands (one cys and two his) provides a favorable geometry for both cuprous and cupric oxidation states. however, the crystal structures of the met121his mutant of azurin from alca ... | 1998 | 9520385 |
| bacterial-fungal biofilms in flowing water photo-processing tanks. | a complex seven species model community, including bacteria and fungi, was selected from organisms isolated from the walls of an industrial flowing water system. growth rates of the species were determined in single and mixed batch culture growth. the rates were found to be significantly higher in mixed culture for pseudomonas alcaligenes and flavobacterium indologenes and higher in single culture for xanthomonas maltophilia, rhodotorula glutinis and fusarium solani, whereas no significant diffe ... | 1998 | 9633659 |
| emendation of genus achromobacter and achromobacter xylosoxidans (yabuuchi and yano) and proposal of achromobacter ruhlandii (packer and vishniac) comb. nov., achromobacter piechaudii (kiredjian et al.) comb. nov., and achromobacter xylosoxidans subsp. denitrificans (rüger and tan) comb. nov. | based on the results of gc content determination and 16s rrna sequence analysis among the type strains of achromobacter xylosoxidans, 4 alcaligenes species, 5 bordetella species, and 12 species of 4 other genera, the separation of genus achromobacter yabuuchi and yano 1981, with the type species achromobacter xylosoxidans, is confirmed. alcaligenes ruhlandii (packer and vishniac) aragno and schlegel 1992 is a distinct species and not a senior synonym of achromobacter xylosoxidans. alcaligenes ru ... | 1998 | 9688077 |
| crystallographic study of azurin from pseudomonas putida. | azurin from pseudomonas putida is a blue copper protein which functions as an electron carrier. two crystal forms of azurin were grown, one in the presence and the other in the absence of zinc acetate; each belongs to space group p21 and contains two molecules per asymmetric unit. the zinc-free crystals have cell dimensions a = 43.25, b = 50.65, c = 54.60 a, beta = 107.79 degrees, while the crystals grown from zinc-containing solution have cell dimensions a = 40.76, b = 51.22, c = 54.96 a, beta ... | 1998 | 9761890 |
| microbiological investigation on black crusts from open-air stone monuments of bologna (italy). | samples of black crusts taken from open-air stone monuments in bologna dating back to between the twelfth and the nineteenth century were considered. the microbiological test procedures isolated from a high number of chemoorganotrophic bacteria, each of the samples although it was expected to find chemo-lithotrophic (specially sulfur-oxidizing and nitrifying) bacteria on account of the great amounts of sulphur, carbon, nitrogen dioxides and ammonia in the air. sporogenous and asporogenous specie ... | 2000 | 10872690 |
| analysis of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pest4011 of achromobacter xylosoxidans subsp. denitrificans strain est4002. | the 2,4-dichlorophenoxyacetic acid (2,4-d)-degradative bacterium achromobacter xylosoxidans subsp. denitrificans strain est4002, isolated in estonia more than 10years ago, was found to contain the 70kb plasmid pest4011 that is responsible for the bacterium having had obtained a stable 2,4-d(+) phenotype. the tfd-like genes for 2, 4-d degradation of the strain est4002 were located on a 10.5kb region of pest4011, but without functional genes coding for chloromuconate cycloisomerase and chlorodiene ... | 2000 | 11024288 |
| distribution of catabolic pathways in some hydrocarbon-degrading bacteria from a subsurface polluted soil. | enrichment cultures on naphtha solvent were used to select aromatic hydrocarbon-degrading bacteria from a btex (benzene, toluene, ethylbenzene, xylene)-contaminated subsoil obtained from beneath a paint factory located in milan, italy. fifteen isolated strains were studied for their different biodegradative capacities. among these, 13 were able to grow on naphtha solvent. ten were identified as pseudomonas putida and three as pseudomonas aureofaciens. two other degraders were identified as pseud ... | 2000 | 11191814 |
| enthalpic and entropic contributions to the mutational changes in the reduction potential of azurin. | the changes in the reduction potential of pseudomonas aeruginosa and alcaligenes denitrificans azurins following point mutations and residue ionizations were factorized into the enthalpic and entropic contributions through variable temperature direct electrochemistry experiments. the effects on the reduction enthalpy due to changes in the first coordination sphere of the copper ion, as in the met121gln and met121his variants of a. denitrificans azurin, insertion of a net charge and alteration in ... | 2001 | 11389584 |
| bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus bordetella. | a novel bordetella species was isolated from an anaerobic, dechlorinating bioreactor culture enriched from river sediment. the only strain, se-1111r(t) (= dsm 12804t = ccug 43448t), for which the name bordetella petrii is proposed, is designated the type strain of the novel species. strain se-1111r(t) was isolated from the dechlorinating mixed culture due to its ability to anaerobically reduce selenate to elemental selenium. comparative 16s rdna sequence analysis showed a close relationship betw ... | 2001 | 11491321 |
| the role of microbial biofilms in deterioration of space station candidate materials. | formation of microbial biofilms on surfaces of a wide range of materials being considered as candidates for use on the international space station was investigated. the materials included a fibre-reinforced polymeric composite, an adhesive sealant, a polyimide insulation foam, teflon cable insulation, titanium, and an aliphatic polyurethane coating. they were exposed to a natural mixed population of bacteria under controlled conditions of temperature and relative humidity (rh). biofilms formed o ... | 1998 | 11541393 |
| soil bacteria pseudomonas putida and alcaligenes xylosoxidans subsp. denitrificans inactivate triclosan in liquid and solid substrates. | triclosan is a broad-spectrum antimicrobial agent that has been incorporated into many household and medical products. bacteria with high levels of triclosan resistance were isolated from compost, water, and soil samples. two of these bacteria, pseudomonas putida triry and alcaligenes xylosoxidans subsp. denitrificans tr1, were able to use triclosan as a sole carbon source and clear particulate triclosan from agar. a decrease in triclosan concentration was measured by hplc within 6 h of inoculat ... | 2001 | 11682176 |
| hydraulic characteristics of an anoxic rotating biological contactor: influence of biofilm. | the hydraulic characteristics of an anoxic rotating biological contactor were studied under different flow rates. the experiments were carried out with the reactor clean (without biomass) and containing denitrifying biofilm (alcaligenes denitrificans) covering the disks. residence time distribution (rtd) experiments were performed by the stimulus-response technique using lithium chloride as tracer. experiments without biomass revealed the existence of hydraulic dead volumes (around 40% for hydra ... | 2001 | 11766038 |
| metabolism of alcaligenes denitrificans in biofilm vs planktonic cells. | to compare the effect of phosphorous concentration (200 mg p l(-1) and 20 mg p l(-1)) on the denitrifying efficiency of alcaligenes denitrificans when in the form of planktonic cells or in the form of a biofilm, and to select the most adequate c/n ratio. | 2002 | 11849353 |
| metabolism of ddt [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] by alcaligenes denitrificans itrc-4 under aerobic and anaerobic conditions. | an isolated bacterium, alcaligenes denitrificans itrc-4, metabolizes 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) under both aerobic and anaerobic conditions. the aerobic metabolism is inhibited by 38% and 47% in the presence of 1.0 g l(-1) of sodium acetate and sodium succinate, respectively, but remains uninhibited in the presence of 1.0 g l(-1) of glucose. also, the metabolism is inhibited completely in the presence of biphenyl vapors, as well as 0.8 g l(-1) of 2,2'-bipyridyl. under an ... | 2003 | 12432467 |
| extraction of exopolymers from biofilms: the protective effect of glutaraldehyde. | the extraction of the exopolymeric matrix is a prerequisite to properly assessing the composition of the biofilm. several extraction methods have already been developed, however, no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. in fact, most of the extraction methods promote leakage of intracellular material. the most common extraction methods, dowex resin and sonication, were assayed in biofilms o ... | 2003 | 12701925 |
| alcaligenes denitrificans n.sp. | | 1954 | 13221772 |
| [meningitis of the newborn due to "alcaligenes denitrificans"]. | | 1961 | 13726343 |
| kerstersia gyiorum gen. nov., sp. nov., a novel alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of alcaligenes denitrificans rüger and tan 1983 as achromobacter denitrificans comb. nov. | a polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. phenotypically, these isolates resembled alcaligenes faecalis. whole-cell protein analysis distinguished two different species, and this was confirmed by dna-dna hybridizations. cellular fatty acid analysis and 16s rdna sequence analysis indicated that these isolates were related to the genera alcaligenes, bordetella, achromobacter and pigmentiphaga and belonged to the family alcaligenaceae ... | 2003 | 14657111 |
| omega-amino acid:pyruvate transaminase from alcaligenes denitrificans y2k-2: a new catalyst for kinetic resolution of beta-amino acids and amines. | alcaligenes denitrificans y2k-2 was obtained by selective enrichment followed by screening from soil samples, which showed omega-amino acid:pyruvate transaminase activity, to kinetically resolve aliphatic beta-amino acid, and the corresponding structural gene (apta) was cloned. the gene was functionally expressed in escherichia coli bl21 by using an isopropyl-beta-d-thiogalactopyranoside (iptg)-inducible pet expression system (9.6 u/mg), and the recombinant apta was purified to show a specific a ... | 2004 | 15066855 |
| use of iron anomalous scattering with multiple models and data sets to identify and refine a weak molecular replacement solution: structure analysis of cytochrome c' from two bacterial species. | the structure of cytochrome c' from two bacterial species, alcaligenes sp and alcaligenes denitrificans, have been determined from x-ray diffraction data to 3.0 a resolution using the anomalous scattering of the single fe atom in each to identify and refine a weak molecular-replacement solution. molecular-replacement studies, with the program amore, used two isomorphous data sets (from the two species), two independent search models (the cytochromes c' from rhodospirillum molischianum and rhodos ... | 1995 | 15299294 |
| structure of the azurin mutant phe114ala from pseudomonas aeruginosa at 2.6 a resolution. | the crystal structure of azurin mutant phe114ala from pseudomonas aeruginosa has been solved by molecular replacement. the final crystallographic r value is 0.185 for 9832 reflections to a resolution of 2.6 a. the root-mean-square deviation for main-chain atom positions is 0.020 a between the four independent monomers in the asymmetric unit. the mutant ala114 crystallized from peg 4000 in a new crystal form and the crystals are monoclinic, p2(1), a= 51.0, b = 83.6, c= 66.4 a and beta = 110.5 deg ... | 1995 | 15299318 |
| structure of apo-azurin from alcaligenes denitrificans at 1.8 a resolution. | the structure of apo-azurin from alcaligenes denitrificans has been determined at high resolution by x-ray crystallography. two separate structure analyses have been carried out, (i) on crystals obtained from solutions of apo-azurin and (ii) on crystals obtained by removal of copper from previously formed crystals of holo-azurin. data to 1.8 a resolution were collected from the apo-azurin crystals, by weissenberg photography (with image plates) using synchrotron radiation and by diffractometry, ... | 1993 | 15299522 |
| three-dimensional structure of cytochrome c' from two alcaligenes species and the implications for four-helix bundle structures. | the three-dimensional structures of two cytochromes c' have been determined in order to analyse the common features of proteins of this family and their relationship with other four-helix bundle structures. the structure of cytochrome c' from alcaligenes sp was determined by molecular replacement supplemented with the iron anomalous scattering and the use of a single isomorphous heavy-atom derivative, and was refined using synchrotron data to 1.8 a resolution. the final model, comprising 956 pro ... | 1996 | 15299707 |
| structure of a new azurin from the denitrifying bacterium alcaligenes xylosoxidans at high resolution. | it has been reported previously that alcaligenes xylosoxidans (nc1mb 11015) grown under denitrifying conditions produces two azurins instead of the single previously identified azurin [dodd, hasnain, hunter, abraham, debenham, kanzler, eldridge, eady, ambler & smith (1995). biochemistry. in the press]. the new azurin, called azurin ii, has been crystallized as blue elongated rectangular prisms with the tetragonal space group p4(1)22 and unit-cell parameters a = b = 52.65, c = 100.63 a. x-ray cry ... | 1995 | 15299774 |
| primary sequence and refined tertiary structure of pseudomonas fluorescens holo azurin at 2.05 a. | this paper reports the primary sequence and refined crystal structure of pseudomonas fluorescens holoazurin. the crystal structure has been determined by molecular replacement on the basis of the molecular model of azurin from alcaligenes denitrificans, and refined by the method of molecular dynamics simulation and energy-restrained least-squares methods. p. fluorescens was crystallized in the orthorhombic space group p2(1)2(1)2(1) with unit-cell dimensions a = 31.95, b = 43.78, c = 78.81 a. the ... | 1997 | 15299879 |
| the completely sequenced plasmid pest4011 contains a novel incp1 backbone and a catabolic transposon harboring tfd genes for 2,4-dichlorophenoxyacetic acid degradation. | the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacterium achromobacter xylosoxidans subsp. denitrificans strain est4002 contains plasmid pest4011. this plasmid ensures its host a stable 2,4-d(+) phenotype. we determined the complete 76,958-bp nucleotide sequence of pest4011. this plasmid is a deletion and duplication derivative of pd2m4, the 95-kb highly unstable laboratory ancestor of pest4011, and was self-generated during different laboratory manipulations performed to increas ... | 2004 | 15489427 |
| establishment of a genetically marked insect-derived symbiont in multiple host plants. | alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (homalodisca coagulata), was transformed using the himar1 transposition system to express egfp. seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time pcr using primers designed to amplify the gene insert. the largest colony of 3,591,427 cells/2 c ... | 2005 | 15723145 |
| production and purification of d-aminoacylase from alcaligenes denitrificans and taxonomic study of the strain. | a d-aminoacylase-producing microorganism, strain da181, isolated from soil was identified as alcaligenes denitrificans subsp. denitrificans. this strain produced about 29,300 units (micromoles of product formed per hour) of d-aminoacylase and 2,300 units of l-aminoacylase per gram of cells (wet weight) when cultivated in a medium containing 1% n-acetyl-dl-leucine as the carbon source. the d-aminoacylase was purified 345-fold. the specific activity of the purified enzyme was 108,600 units per mg ... | 1988 | 16347618 |
| production of d-aminoacylase from alcaligenes denitrificans subsp. xylosoxydans mi-4. | a bacterial strain that produces d-aminoacylase was isolated from soil and identified as alcaligenes denitrificans subsp. xylosoxydans mi-4. l-aminoacylase activity in this strain was only 1 to 2% of d-aminoacylase activity. d-aminoacylase was inducibly produced. n-acetyl-dl-leucine was the best inducer, and the d-isomer had the ability to induce the enzyme. enzymatic resolution of n-acetyl-dl-methionine with the crude enzyme was carried out, and the d/l ratio in the resolved methionine was appr ... | 1988 | 16347776 |
| determinants encoding resistance to several heavy metals in newly isolated copper-resistant bacteria. | three copper-resistant, gram-negative bacteria were isolated and characterized. of the three strains, alcaligenes denitrificans ah tolerated the highest copper concentration (mic = 4 mm cuso(4)). all three strains showed various levels of resistance to other metal ions. a. denitrificans ah contains sequences which cross-hybridized with the mer (mercury resistance) determinant of tn21 and the czc (cobalt, zinc, and cadmium resistance), cnr (cobalt and nickel resistance), and chr (chromate resista ... | 1991 | 16348575 |