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identification of nonfermentative gram-negative bacteria in the clinical laboratory.a simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. these organisms included apyocyanogenic pseudomonas aeruginosa, p. fluorescens, p. putida, p. stutzeri, p. maltophilia, p. putrefaciens, p. cepacia, p. alcaligenes, flavobacterium species, bordetella bronchiseptica, acinetobacter anitratum (herellea vaginicola), a. iwoffi (mima poly ...1975163060
gentamicin resistance in christchurch hospitals.prior to june 1976, the isolation of gentamicin resistant organisms was an infrequent occurrence in north canterbury hospital board institutions. during july 1976, 20 different gentamicin resistant organisms were isolated from patients in christchurch hospital. gentamicin resistant organisms hav e been continually isolated from an increasingly wide area since then. the organisms involved are: providence species; pseudomonas aeruginosa; klebsiella species; e coli; staphyloccus aureus; proteus mir ...1979381994
evaluation of the 4-hour rapid nf plus method for identification of 345 gram-negative nonfermentative rods.the ability of the rapid nf plus system (innovative diagnostic systems, inc., atlanta, ga.) to identify 345 nonfermentative gram-negative rods was evaluated. kits were inoculated with no. 1 mcfarland suspensions, and reactions were interpreted after a 4-h incubation at 35 degrees c. overall, the method correctly identified 311 strains (90.1%) without additional tests and 21 strains (6.1%) with additional tests, and 13 strains (3.8%) were misidentified. five of 13 misidentified strains were alcal ...19921583129
comparison of rapid nft system and conventional methods for identification of nonsaccharolytic gram-negative bacteria.this study examined the rapid nft system (analytab products, plainview, n.y.) to determine its ability to accurately identify 229 clinical isolates of mostly nonsaccharolytic gram-negative rods. identifications were classified by the following scheme: correct (corresponding to excellent, very good, good, or acceptable identification as listed in the code book); low discrimination (correct identification among a range of listed possibilities, with additional tests necessary for accurate identific ...19863536999
alcaligenes odorans, varietas viridans. 19714400126
selection of r- and s-phase colonies from dimorphic alcaligenes odorans var. viridans, and their isolation in pure culture. 19734579137
"alcaligenes odorans var. viridans" isolated from human sources. 19734774756
[nitrite reduction by alcaligenes odorans var. viridans]. 19695366475
characteristics of alcaligenes odorans var. viridans isolated from human sources. 19676036895
alcaligenes odorans, var. viridans as a hospital infectant.the hazards of acquiring a nosocomial infection are significant even in modern hospitals in spite of aseptic procedures and antibiotics. in order to prevent or stop a hospital epidemic it is necessary to know or to recognize the infective agent, its source and its way of spreading. this is not always easy, esp. with some potential pathogens which are frequently found in the pathological materials as well as in the hospital environment. in the described series of hospital spread of alcaligenes od ...19816784393
[staghorn renal lithiasis treated with shock waves. bacteriologic aspects].struvite renal stones are caused by infection of the urine with bacteria that synthesize the enzyme urease. ammonium is released by the breakdown of urea by urease, the urine becomes highly alkaline, and magnesium ammonium phosphate (struvite) and carbonate apatite crystallize. incorporation of the infecting bacteria within the developing stone, results in a focus of infection that is resistant to conventional antimicrobial therapy, and which is manifested clinically by repeated urinary tract in ...19947658975
epidemiological investigation of infections due to alcaligenes species in children and patients with cystic fibrosis: use of repetitive-element-sequence polymerase chain reaction.twenty-one isolates of alcaligenes species were recovered from the respiratory tract of 16 patients at texas children's hospital over a 1-year period. all but one were identified as alcaligenes xylosoxidans; the remaining isolate was identified as alcaligenes faecalis (formerly alcaligenes odorans). thirteen of 21 isolates were from the sputum of eight patients with cystic fibrosis (cf), two of whom were persistently colonized. the remaining isolates were recovered from intubated children. patte ...19957795082
taxonomy of the species pseudomonas odorans. 196314114922
multiarray sensors with pattern recognition for the detection, classification, and differentiation of bacteria at subspecies and strain levels.this work describes the integration of a fully autonomous electrochemical biosensor with pattern recognition techniques for the detection and classification of bacteria at subspecies and strain level. the system provides a continuous, real-time monitoring of bacteria activity upon exposure to antibiotics. the system utilizes 96-well-type electrodes array (dox-dissolved oxygen sensor) with principal component analysis (pca) for rapid and routine classification of different classes of bacteria and ...200516351141
draft genome sequence of alcaligenes faecalis subsp. faecalis ncib 8687 (ccug 2071).alcaligenes faecalis subsp. faecalis ncib 8687, the betaproteobacterium from which arsenite oxidase had its structure solved and the first "arsenate gene island" identified, provided a draft genome of 3.9 mb in 186 contigs (with the largest 15 comprising 90% of the total) for this opportunistic pathogen species.201222933773
paenalcaligenes hominis gen. nov., sp. nov., a new member of the family alcaligenaceae.a beige-pigmented bacterium (strain ccug 53761a(t)) was isolated from human blood from an 85-year-old man in göteborg, sweden. comparative analysis of 16s rrna gene sequences showed that this bacterium displayed <95 % similarity to all described species of the genera of the family alcaligenaceae. it grouped within the radiation of the genus alcaligenes, but showed only 93.0-94.8 % similarity to type strains of members of this genus (alcaligenes faecalis subsp. parafaecalis, 94.8 %; alcaligenes f ...201019684310
linear alkylbenzene sulfonate tolerance in bacteria isolated from sediment of tropical water bodies polluted with detergents.the discharge of untreated detergent-bearing waste introduces linear alklcylbenzene sulfonates (las) to the aquatic environment. the surfactant persists in some streams and rivers in nigeria, some is adsorbed to suspended materials and end in the sediment of the receiving water bodies. in this study, bacteria isolated from sediments of some tropical detergent-effluent-polluted streams were tested for tolerance to las using the media dilution technique. las-tolerance was indicated by growth of th ...200819419067
kinetic explanation for accumulation of nitrite, nitric oxide, and nitrous oxide during bacterial denitrification.the kinetics of denitrification and the causes of nitrite and nitrous oxide accumulation were examined in resting cell suspensions of three denitrifiers. an alcaligenes species and a pseudomonas fluorescens isolate characteristically accumulated nitrite when reducing nitrate; a flavobacterium isolate did not. nitrate did not inhibit nitrite reduction in cultures grown with tungstate to prevent formation of an active nitrate reductase; rather, accumulation of nitrite seemed to depend on the relat ...198116345900
[isolation of bacteria which could perform nitrification and denitrification simultaneously by gene probe].the bacteria which could perform nitrification and denitrification simultaneously from nitrogen containing wastes in taiwan were isolated by using the probes made from random dna fragments of thiosphaera pantotropha. two isolates were identified and named alcaligenes faecalis subsp. faecalis strain 1 and strain 2 respectively. the effects on nitrification and denitrification by different medium ph, oxygen content, addition of different electron donors or inhibitors were studied. the isolates not ...199710592830
degradation of crude oil by acinetobacter calcoaceticus and alcaligenes odorans.two types of indian crude oil (bombay high and gujarat) were tested for their biodegradability by acinetobacter calcoaceticus and alcaligenes odorans. acinetobacter calcoaceticus s30 and alc. odorans p20 degraded bombay high crude oil by 50% and 45%, while only 29% and 37% of gujarat crude oil (heavy crude oil) was degraded by these isolates, respectively. acinetobacter calcoaceticus and alc. odorans in combination degraded 58% and 40% of bombay high and gujarat crude oils, respectively, which w ...19968896350
[nitrate, nitrite and nitrous oxide reduction by alcaligenes denitrificans and alcaligenes odorans]. 19734792486
comparison of alcaligenes faecalis and alcaligenes odorans var. viridans by carbon source utilization test. 19744432775
susceptibility of nonfermentative gram-negative bacilli to tobramycin.there has been increasing interest in the pathogenic role of nonfermentative gram-negative bacilli in human infections. except for pseudomonas aeruginosa, the susceptibility pattern of these organisms to tobramycin has not been evaluated thoroughly. the activity of tobramycin, as compared with that of gentamicin, was tested by the serial broth dilution technique against 178 isolates of nonfermentative gram-negative bacilli obtained from various sources. p. aeruginosa, pseudomonas stutzeri, acine ...1976823278
carbon substrate utilization studies of some cultures of alcaligenes denitrificans, alcaligenes faecalis, and alcaligenes odorans isolated from clinical specimens.one hundred and sixty-two cultures of alcaligenes species (a. denitrificans, a. faecalis, and a. odorans) of clinical origin were characterized by routine diagnostic and carbon substrate utilization techniques. the microorganisms were tested for their ability to utilize a total of 188 substrates. substrate utilization was assayed by (i) growth stimulation and (ii) substrate alkalinization. the a. denitrificans and a. odorans cultures had unique substrate utilization profiles for each species. th ...1978701468
evaluation of the oxi/ferm tube system with selected gram-negative bacteria.the oxi/ferm test system was evaluated for accuracy and reliability for identification of nonfermentative and oxidase-positive fermentative bacteria by using 375 bacterial strains obtained from stock culture and clinical specimens. the oxi/ferm system is a compartmentalized tube containing eight media to provide nine biochemical test results. when combined with the oxidase test, the results corresponding to the positive reactions are totaled and the composite number is located in the coding manu ...1977338624
degradation of polychlorinated biphenyls by mixed microbial cultures.three different enriched mixed cultures capable of degrading polychlorinated biphenylas were isolated from two soil samples and a river sediment, respectively. the predominant organisms found in all three mixed cultures were alcaligenes odorans, alcaligenes dentrificans, and an unidentified bacterium. the polychlorinated biphenyl isomers that were more water soluble and had lower chlorination were not only degraded at a faster rate than those that were less water soluble and had higher chlorinat ...1979110265
characterization of alcaligenes faecalis strain ad15 indicating biocontrol activity against plant pathogens.bacterial strain possessing both bacteriostatic and fungistatic activity (biocontrol activity) against pathogens of cyclamen (cyclamen sp.) was isolated from the soil in gifu prefecture, japan, and characterized with respect to its taxonomic and biocontrol properties. the sequence of its 16s rrna gene, morphology, biochemistry, and fatty acid composition demonstrated that it is a strain most closely related to alcaligenes faecalis subsp. faecalis lmg 1229(t). the isolate was named a. faecalis st ...201323759862
paenalcaligenes suwonensis sp. nov., isolated from spent mushroom compost.a bacterial strain, abc02-12(t), was isolated from spent mushroom compost, a waste product of button mushroom cultivation. cells of the strain were gram-stain-negative, catalase- and oxidase-positive, non-spore-forming, aerobic flagellated rods. optimum growth occurred at 28 °c and ph 7.0. 16s rrna gene sequence analysis showed that strain abc02-12(t) shared the highest sequence similarities with paenalcaligenes hominis ccug 53761a(t) (96.0 %), alcaligenes faecalis subsp. parafaecalis g(t) (95.7 ...201424271214
alcaligenes endophyticus sp. nov., isolated from roots of ammodendron bifolium.a gram-staining negative, rod-shaped, motile bacterium, designated aer10t, was isolated from roots of ammodendron bifolium collected from takeermohuer desert in xinjiang uygur autonomous region, north western china. growth was found to occur from 10°c to 45°c, at ph 5.0-9.0 and could tolerate nacl up to 10 % (w/v). 16s rrna gene sequence result indicated that the strain aer10t belongs to the genus alcaligenes and was closely related to alcaligenes aquatilis (98.4 %), alcaligenes faecalis subsp. ...201627959788
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