| helcococcus, a new genus of catalase-negative, gram-positive cocci isolated from clinical specimens. | | 1995 | 7650202 |
| phylogenetic analysis of some aerococcus-like organisms from clinical sources: description of helcococcus kunzii gen. nov., sp. nov. | 16s rrna gene sequencing studies were performed on some unusual gram-positive catalase-negative cocci of unknown taxonomic position isolated from human clinical sources. comparative analysis of the sequence data demonstrated that the clinical isolates represent a hitherto-unknown line of descent within the low-g+c-content gram-positive bacteria. on the basis of the phylogenetic findings and the phenotypic distinctiveness of the organisms, it is proposed that they be classified in a new genus, he ... | 1993 | 8347503 |
| helcococcus kunzii as sole isolate from an infected sebaceous cyst. | helcococcus kunzii was isolated in pure culture from pus drained from an infected sebaceous cyst associated with marked cellulitis. the cyst was excised one month later after the inflammation had subsided with flucloxacillin treatment. this is the first report of the isolation of h. kunzii as the sole pathogen from an infected site. | 1997 | 8968941 |
| colonization of skin by helcococcus kunzii. | in order to investigate the role of helcococcus kunzii as a colonizer of skin and as a possible participant in diabetic foot ulcers, we used a selective medium to culture both lower- and upper-extremity skin from a study group of podiatry patients (60 diabetics and 60 nondiabetics) and a control group of 50 healthy volunteers. although differences in colonization were not statistically significant, a trend toward higher colonization rates in the group of podiatry patients was noted. h. kunzii ap ... | 1997 | 9350728 |
| breast abscess associated with helcococcus kunzii. | helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. we report a case of breast abscess associated with h. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susceptibility testing for this species. | 1998 | 9666030 |
| helcococcus ovis sp. nov., a gram-positive organism from sheep. | two strains of a hitherto undescribed gram-positive, catalase-negative, facultatively anaerobic coccus isolated from sheep were characterized by phenotypic and molecular taxonomic methods. comparative 16s rrna gene sequencing studies demonstrated that the unknown strains were genealogically highly related and constitute a new line close to, but distinct from, helcococcus kunzii. the unknown bacterium was readily distinguished from h. kunzii by biochemical tests and electrophoretic analysis of wh ... | 1999 | 10555322 |
| immunoglobulin a1 protease activity in gemella haemolysans. | the purpose of this study was to determine the occurrence and nature of immunoglobulin a1 (iga1) protease activity in members of the genus gemella and related taxa. among a total of 22 gemella strains belonging to the four species gemella haemolysans, gemella morbillorum, gemella sanguinis, and gemella bergeriae and four reference strains of the species helcococcus kunzii, facklamia hominis, and globicatella sanguinis, iga1 protease activity was an exclusive character of all nine isolates of g. ... | 2000 | 10878081 |
| hemagglutinin of unusual specificity from helcococcus kunzii. | helcococcus kunzii is a gram-positive, catalase-negative opportunist. the organism has been isolated from the lower extremities and breast masses of several patients. a clinical isolate of helcococcus kunzii was shown to possess a hemagglutinin-lectin with a specificity for n-acetylglucosamine and lactose, two structurally unrelated carbohydrates. the lectin is sensitive to protease, heat and mutanolysin. electron microscopy failed to reveal fimbriae or fibrillae, suggesting that the lectin is a ... | 2002 | 11807570 |
| reclassification of clostridium hydroxybenzoicum as sedimentibacter hydroxybenzoicus gen. nov., comb. nov., and description of sedimentibacter saalensis sp. nov.. | strain zf2t, isolated from freshwater sediment, is a motile, rod-shaped, gram-positive, endospore-forming, amino acid- and pyruvate-utilizing, anaerobic bacterium. it requires yeast extract for growth. carbohydrates are not utilized. the optimal temperature and ph for growth are 37 degrees c and 6.8-7.3, respectively. the g+c content of the dna is 34.0 mol %. a 16s rdna sequence analysis of strain zf2t revealed that the highest similarity (94.4%) was shared with clostridium hydroxybenzoicum jw/z ... | 2002 | 12054241 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| valvular endocarditis associated with helcococcus ovis infection in a bovine. | a 12-month-old angus bull calf with a history of fever and lethargy of several weeks' duration was necropsied. macroscopic findings included general dehydration, congestion, and edema within the craniodorsal lobes of the lung, multifocal ecchymotic hemorrhages on the dorsal epaxial and gluteal muscles, bloody ingesta within the gastrointestinal tract, and a 4- x 4- x 5-cm irregular plaque located on the right atrioventricular heart valve. microscopically, there were focally extensive pulmonary a ... | 2003 | 14535550 |
| isolation of helcococcus kunzii from a post-surgical foot abscess. | we report here a case in which a strain of helcococcus kunzii was isolated from a foot abscess, in an immunocompetent patient, after surgery for metatarsal fracture. correct species identification of the strain was readily obtained on the basis of morphological and biochemical features, together with 16s rrna analysis. this case demonstrates that this species should be considered a potential pathogen in patients with no underlying disease. | 2003 | 14760975 |
| helcococcus ovis isolated from a pulmonary abscess in a horse. | helcococcus ovis, a recently described organism cultured from sheep, was isolated in pure culture from a pulmonary abscess in a horse. this is the first report of this organism in horses and the first report in veterinary medicine to clearly demonstrate a pathogenic role for this organism. | 2004 | 15131196 |
| prosthetic joint infection due to "helcococcus pyogenes" [corrected]. | helcococci have previously been associated with the colonization of ulcers and infections of the skin and soft tissues. we describe a case of prosthetic joint infection due to a previously undescribed organism that is genetically most closely related to helcococcus. | 2004 | 15184496 |
| helcococcus sueciensis sp. nov., isolated from a human wound. | a previously undescribed, gram-positive, catalase-negative, coccus-shaped organism that originated from a human wound was subjected to taxonomic study. on the basis of its cellular morphology and the results of biochemical testing, the unknown organism was identified tentatively as a member of the genus helcococcus, but it did not correspond to either of the two recognized species of this genus. comparative 16s rrna gene sequencing studies confirmed that the bacterium was associated phylogenetic ... | 2004 | 15388710 |
| a novel helcococcus-like organism causing endocarditis in an injecting drug user. | a case is presented of infective endocarditis caused by a previously undescribed helcococcus-like organism, in a patient with human immunodeficiency virus infection. the organism, which could not be identified phenotypically in the routine laboratory, was identified using partial 16s rrna gene sequencing. | 2005 | 16245510 |
| life-threatening invasive helcococcus kunzii infections in intravenous-drug users and erma-mediated erythromycin resistance. | we report the first two cases of life-threatening invasive helcococcus kunzii infection, with primary bacteremia and empyema thoracis, respectively. gram smears of both h. kunzii isolates showed a mixture of gram-positive and gram-negative cocci. the isolate from the first patient, resistant to erythromycin and clindamycin, possessed an erma gene. | 2005 | 16333132 |
| isolation of helcococcus kunzii from plantar phlegmon in a vascular patient. | helcococcus kunzii has previously been considered to belong to the normal skin flora of podiatry patients. here, h. kunzii was isolated in abundance from a pus specimen collected by incision and drainage of plantar phlegmon. this fastidious gram-positive species was unambiguously identified with the colorimetric vitek 2 gp card identification system. this suggests that this phenotypic identification system is able to identify promptly h. kunzii, which should be considered a potential pathogen. | 2008 | 18566154 |
| helcococcus ovis, an emerging pathogen in bovine valvular endocarditis. | the initial isolation of helcococcus ovis from a valvular thrombus prompted us to investigate the prevalence of this bacterium in bovine valvular endocarditis. specimens from 55 affected hearts were examined by culture using columbia blood agar and cross streaking the inoculated plate with a staphylococcus aureus strain. as confirmed by 16s rrna gene sequencing, h. ovis was isolated with an unexpectedly high frequency of 33%, predominantly as heavy growth and pure culture. the majority of h. ovi ... | 2008 | 18716228 |
| isolation of helcococcus ovis from sheep with pleuritis and bronchopneumonia. | helcococcus ovis is a newly established species in the genus helcococcus. the clinical significance of this organism in sheep has not been reported. in the current report, isolation of h. ovis from a 6-month-old mixed-breed ewe lamb that died of respiratory disease is described. pathologic examination revealed severe, focally extensive, chronic necrotizing pleuritis with intralesional coccobacilli and mild, multifocal, subacute mucopurulent bronchopneumonia, indicating a bacterial etiology. a gr ... | 2009 | 19139523 |
| murdochiella asaccharolytica gen. nov., sp. nov., a gram-stain-positive, anaerobic coccus isolated from human wound specimens. | two strains of previously unknown gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. comparative 16s rrna gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, wal 1855c(t) and wal 2038e, are genotypically homogeneous and constitute a novel lineage within clostridium cluster xiii. there was 13-14 % 16s rrna gene sequence divergence bet ... | 2010 | 19666803 |
| helcococcus kunzii isolated from a sow with purulent urocystitis. | helcococcus kunzii has never been reported in veterinary medicine. the isolation of h. kunzii from a sow with purulent urocystitis is described, suggesting this organism's potential pathogenic role in swine. | 2010 | 20534802 |
| surface bacteriology of venous leg ulcers and healing outcome. | bacteria can be cultured from all venous leg ulcers (vlus) regardless of healing status, and the significance of a positive swab result in non-clinically infected ulcers is unknown. the aim of this study was to characterise the bacteriological flora of vlus by routine culture to determine whether the data generated had prognostic value. | 2010 | 20671048 |
| antimicrobial susceptibilities and occurrence of resistance genes in bovine helcococcus ovis isolates. | the aim of the study was to investigate antimicrobial susceptibilities of bovine helcococcus ovis isolates and to detect genes encoding for h. ovis erythromycin and tetracycline resistance. twenty-nine isolates were included and the minimal inhibitory concentrations (mics) of seven antimicrobials were determined using test conditions as given in an approved clsi guideline for the pyridoxal-dependent abiotrophia spp. and granulicatella spp. furthermore, the macrolide resistance phenotype was exam ... | 2010 | 21131144 |
| the challenges of implantable cardiac device infection due to helcococcus kunzii. | | 2011 | 21676496 |
| Prosthetic joint infection caused by Helcococcus kunzii. | Helcoccus kunzii was isolated by sonication and conventional cultures obtained from a case of infection following total knee prosthesis in an immunocompetent patient. The patient recovered uneventfully. This is the first known case of a H. kunzii prosthetic joint infection . | 2011 | 22135250 |
| Hot topic: Bovine milk samples yielding negative or nonspecific results in bacterial culturing-The possible role of PCR-single strand conformation polymorphism in mastitis diagnosis. | A large proportion of mastitis milk samples yield negative or nonspecific results (i.e., no mastitis pathogen can be identified) in bacterial culturing. Therefore, the culture-independent PCR-single strand conformation polymorphism method was applied to the investigation of bovine mastitis milk samples. In addition to the known mastitis pathogens, the method was suitable for the detection of fastidious bacteria such as Mycoplasma spp., which are often missed by conventional culturing methods. Th ... | 2012 | 22192188 |