| enrichment and isolation of acetitomaculum ruminis, gen. nov., sp. nov.: acetogenic bacteria from the bovine rumen. | five strains of acetogenic bacteria were isolated by selective enrichment from the rumen of a mature hereford crossbred steer fed a typical high forage diet. suspensions of rumen bacteria, prepared from contents collected 7 h postfeeding, blended and strained through cheesecloth, were incubated in a minimal medium containing 10% clarified rumen fluid under either h2:co2 (80:20) or n2:co2 (80:20) headspace atmosphere. the selection criterion was an increment of acetate in the enrichments incubate ... | 1989 | 2500921 |
| assignment of the agent of tyzzer's disease to clostridium piliforme comb. nov. on the basis of 16s rrna sequence analysis. | the small-subunit rrna (16s rrna) sequence of tyzzer's bacillus (also known as "bacillus piliformis") was elucidated by using the polymerase chain reaction followed by reverse transcriptase sequencing. by using maximum-likelihood analysis, a phylogenetic tree was constructed from this and other 16s rrna sequences available from the first release of the ribosomal database project (g. j. olsen, r. overbeek, n. larsen, t. l. marsh, m. j. mccaughey, m. a. maciukenas, w.-m. kuan, t. j. macke, y. xing ... | 1993 | 7684241 |
| phylogeny of the ammonia-producing ruminal bacteria peptostreptococcus anaerobius, clostridium sticklandii, and clostridium aminophilum sp. nov. | in previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. three isolates, strains c, ft (t = type strain), and sr, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. on the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. in this study, the 16s rrna sequences of these isolat ... | 1993 | 8427801 |
| the effects of co-cultivation with the acetogen acetitomaculum ruminis on the fermentative metabolism of the rumen fungi neocallimastix patriciarum and neocallimastix sp. strain l2. | the effects of co-cultivation with the hydrogen-utilizing acetogenic bacterium acetitomaculum ruminis on the fermentative activities of the rumen fungi neocallimastix patriciarum or neocallimastix sp. l2 were investigated. in both co-cultures acetate production increased, making it the predominant fermentation product, as the accumulation of lactate, formate, ethanol, h2 and (in the case of neocallimastix sp. l2) succinate all decreased. the effects of co-cultivation with methanobrevibacter smit ... | 1995 | 8566705 |
| assessment of reductive acetogenesis with indigenous ruminal bacterium populations and acetitomaculum ruminis. | the objective of this study was to evaluate the role of reductive acetogenesis as an alternative h2 disposal mechanism in the rumen. h2/co2-supported acetogenic ruminal bacteria were enumerated by using a selective inhibitor of methanogenesis, 2-bromoethanesulfonic acid (bes). acetogenic bacteria ranged in density from 2.5 x 10(5) cells/ml in beef cows fed a high-forage diet to 75 cells/ml in finishing steers fed a high-grain diet. negligible endogenous acetogenic activity was demonstrated in in ... | 1998 | 9726893 |
| [sugar phosphorylation activities in acetogenic bacteria]. | seven acetogenic bacteria (acetitomaculum ruminis, acetobacterium woodii, eubacterium limosum as well as isolates a2, a4, a10 and h3hh) were tested for pep- and atp-dependent phosphorylation of glucose and 2-deoxyglucose. although all organisms had detectable phosphorylation activity, substantial variation existed in the rates of both pep- and atp-dependent phosphorylation. isolate alo had the highest rate of pep-dependent phosphorylation of 11.62 nmol.l-1.mg-1.min-1. isolate a10, h3hh as well a ... | 1999 | 12555560 |