| phylogenetic inferences and taxonomic consequences of 16s ribosomal dna sequence comparison of chromohalobacter marismortui, volcaniella eurihalina, and deleya salina and reclassification of v. eurihalina as halomonas eurihalina comb. nov. | the phylogenetic positions of the moderately halophilic bacteria chromohalobacter marismortui, volcaniella eurihalina, and deleya salina were determined by pcr amplification of rrna genes and direct sequencing. the resulting data were compared with data for other bacteria obtained from 16s rrna sequence databases. c. marismortui, v. eurihalina, and d. salina clustered phylogenetically within the gamma subclass of the proteobacteria and are closely related to other species on the halomonas-deleya ... | 1995 | 7547290 |
| phylogenetic relationships between some members of the genera deleya, halomonas, and halovibrio. | the genera halomonas and deleya, which constitute the family halomonadaceae, are difficult to differentiate on the basis of phenotypic and chemotaxonomic attributes. dna-rrna hybridization studies have indicated that some halomonas spp. have the same level of relationship to the type species of the genus deleya as some deleya spp. a phylogenetic analysis of the 16s rrna sequences of seven members of the halomonadaceae indicated that the members of the genera halomonas and deleya do not form sepa ... | 1993 | 8240950 |
| characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16s rdna sequences. | one hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophylic conditions from arctic (98 strains) and antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. by numerical analysis, 8 clusters, containing 2 to 59 strains, could be delineated, and 8 strains formed separate branches. five clusters contained strains from both poles, two minor clusters were confined to arctic ... | 2001 | 11403404 |
| temporal stability and biodiversity of two complex antilisterial cheese-ripening microbial consortia. | the temporal stability and diversity of bacterial species composition as well as the antilisterial potential of two different, complex, and undefined microbial consortia from red-smear soft cheeses were investigated. samples were collected twice, at 6-month intervals, from each of two food producers, and a total of 400 bacterial isolates were identified by fourier-transform infrared spectroscopy and 16s ribosomal dna sequence analysis. coryneform bacteria represented the majority of the isolates ... | 2003 | 12839776 |
| halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a bolivian hypersaline lake. | halomonas boliviensis sp. nov. is proposed for two moderately halophilic, psychrophilic, alkalitolerant bacteria, lc1(t) (=dsm 15516(t)=atcc baa-759(t)) and lc2 (=dsm 15517=atcc baa-760), both of which were isolated from a soil sample around the lake laguna colorada, located at 4300 m above sea level in the south-west region of bolivia. the bacteria are aerobic, motile, gram-negative rods that produce colonies with a cream pigment. moreover, they are heterotrophs that are able to utilize various ... | 2004 | 15143014 |
| comparative phylogenetic analyses of halomonas variabilis and related organisms based on 16s rrna, gyrb and ectbc gene sequences. | halomonas variabilis and phylogenetically related organisms were isolated from various habitats such as antarctic terrain and saline ponds, deep-sea sediment, deep-sea waters affected by hydrothermal plumes, and hydrothermal vent fluids. ten strains were selected for physiological and phylogenetic characterization in detail. all of those strains were found to be piezotolerant and psychrotolerant, as well as euryhaline halophilic or halotolerant. their stress tolerance may facilitate their wide o ... | 2004 | 15214638 |
| extremely halophilic denitrifying bacteria from hypersaline inland lakes, halovibrio denitrificans sp. nov. and halospina denitrificans gen. nov., sp. nov., and evidence that the genus name halovibrio fendrich 1989 with the type species halovibrio variabilis should be associated with dsm 3050. | anaerobic enrichments with acetate as electron donor and nitrate as electron acceptor at 4 m nacl from inland, hypersaline lake sediments from central asia resulted in the isolation of several extremely halophilic bacteria that comprised two subgroups, most with vibrio-shaped cells and a single strain with rod-shaped cells. members of both subgroups were extremely halophilic, with growth occurring in 2-5 m nacl with an optimum at 2-3 m. 16s rrna gene sequence analysis showed a close affiliation ... | 2006 | 16449444 |
| the status of the genus name halovibrio fendrich 1989 and the identity of the strains pseudomonas halophila dsm 3050 and halomonas variabilis dsm 3051. request for an opinion. | during the course of studies on halophilic, gram-negative bacteria, a comparison of two strains of halophilic bacteria, pseudomonas halophila dsm 3050 and halomonas variabilis dsm 3051 (formerly halovibrio variabilis) demonstrated that the characteristics of strain dsm 3050 corresponded to the original description of halovibrio variabilis and those of dsm 3051 to p. halophila, both of which had been isolated from the great salt lake in utah [fendrich, c. (1988). syst appl microbiol 11, 36-43]. i ... | 2006 | 16449462 |
| cloning and expression of a 5-enolpyruvylshikimate-3-phosphate synthase gene from halomonas variabilis. | a novel 5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene of 1.35 kb was cloned from a cosmid library of halomonas variabilis htg7, inserted into vector pet-28a (+) and transformed in escherichia coli bl21 (de3). epsps was over-expressed in soluble form after induction with iptg at 30 degrees c and it showed a single band in sds-page, which corresponds to a molecular weight of 51 kd. deduced amino acid sequence analysis showed that there is little homology with the aroa genes which encode ... | 2006 | 17286049 |
| halomonas titanicae sp. nov., a halophilic bacterium isolated from the rms titanic. | a gram-negative, heterotrophic, aerobic, non-endospore-forming, peritrichously flagellated and motile bacterial strain, designated bh1(t), was isolated from samples of rusticles, which are formed in part by a consortium of micro-organisms, collected from the rms titanic wreck site. the strain grew optimally at 30-37°c, ph 7.0-7.5 and in the presence of 2-8 % (w/v) nacl. we carried out a polyphasic taxonomic study in order to characterize the strain in detail. phylogenetic analyses based on 16s r ... | 2010 | 20061494 |
| metaproteomic and metagenomic analyses of defined oceanic microbial populations using microwave cell fixation and flow cytometric sorting. | a major obstacle in the molecular investigation of natural, especially oceanic, microbial cells is their adequate preservation for further land-based molecular analyses. here, we examined the use of microwaves for cell fixation before high-speed flow cytometric sorting to define the metaproteomes and metagenomes of key microbial populations. the microwave fixation procedure was established using cultures of synechococcus cyanobacteria, the photosynthetic eukaryote micromonas pusilla and the gamm ... | 2010 | 20618860 |
| anaerobic degradation of textile dye bath effluent using halomonas sp. | the main objective of this work is to reduce the chemical oxygen demand (cod) and color of the effluent containing reactive textile dye by microbial method. anaerobic digestion has the potential to break down complex refractory organic compounds so that they may be further degraded aerobically or to completely mineralize them. an anaerobic digestion technique was applied to synthetic reactive red 2 dye cotton textile effluent aiming at the dye degradation. halophilic and halotolerant bacterial c ... | 2011 | 21463931 |
| diverse microhabitats experienced by halomonas variabilis on salt-secreting leaves. | the leaf surfaces of the salt-excreting tree tamarix aphylla harbor a wide diversity of halophilic microorganisms, including halomonas sp., but little is known of the factors that shape community composition in this extreme habitat. we isolated a strain of halomonas variabilis from the leaf surface of t. aphylla and used it to determine the heterogeneity of salt concentrations experienced by bacteria in this environment. this halophilic strain was transformed with a prou::gfp reporter gene fusio ... | 2013 | 23160133 |
| halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil. | a gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain bh103t, was isolated from saltern soil in gomso, korea. cells of strain bh103t were strictly aerobic, motile, straight rods and grew at ph 7.0-10.8 (optimum, ph 8.5), at 10-55 °c (optimum, 28 °c) and at salinities of 0-23 % (w/v) nacl (optimum, 14 % nacl). phylogenetic analyses based on 16s rrna gene sequences indicated that strain bh103t belongs to the genus halomonas, showing highest sequence similarity to halo ... | 2015 | 26431725 |
| the designated type strain of pseudomonas halophila fendrich 1989 is dsm 3051, the designated type strain of halovibrio variabilis fendrich 1989 is dsm 3050, the new name halomonas utahensis (fendrich 1989) sorokin and tindall 2006 is created for the species represented by dsm 3051 when treated as a member of the genus halomonas, the combination halomonas variabilis (fendrich 1989) dobson and franzmann 1996 is rejected, and the combination halovibrio denitrificans sorokin et al. 2006 is validly published with an emendation of the description of the genus halovibrio fendrich 1989 emend. sorokin et al. 2006. opinion 93. judicial commission of the international committee on systematics of prokaryotes. | the judicial commission affirms that, according to information presented to it, the designated type strain of pseudomonas halophila fendrich 1989 is dsm 3051 (replacing dsm 3050) and the designated type strain of halovibrio variabilis fendrich 1989 is dsm 3050 (replacing dsm 3051). a new name, halomonas utahensis (fendrich 1989) sorokin and tindall 2006 nom. nov., is created for the species represented by dsm 3051 when treated as a member of the genus halomonas, because the combination halomonas ... | 2014 | 25288663 |
| halomonas nanhaiensis sp. nov., a halophilic bacterium isolated from a sediment sample from the south china sea. | a novel gram-negative, aerobic, slightly halophilic, yellow-pigmented, oxidase-negative, voges-proskauer positive, non-spore-forming bacterium, designated yim m 13059(t), was isolated from a sediment sample collected from the south china sea at a depth of 310 m. optimal growth was found to occur at 28-30 °c, ph 7.0 and in the presence of 3-4 % (w/v) nacl. cells were observed to be rod-shaped and motile by peritrichous flagella. the polar lipids of strain yim m 13059(t) were found to be diphospha ... | 2013 | 23314928 |
| bacterial exopolysaccharide and biofilm formation stimulate chickpea growth and soil aggregation under salt stress. | to compensate for stress imposed by salinity, biofilm formation and exopolysaccharide production are significant strategies of salt tolerant bacteria to assist metabolism. we hypothesized that two previously isolated salt-tolerant strains halomonas variabilis (ht1) and planococcus rifietoensis (rt4) have an ability to improve plant growth, these strains can form biofilm and accumulate exopolysacharides at increasing salt stress. these results showed that bacteria might be involved in developing ... | 2012 | 24031943 |