| nucleotide sequence of shallot virus x rna reveals a 5'-proximal cistron closely related to those of potexviruses and a unique arrangement of the 3'-proximal cistrons. | the 8890 nucleotide rna sequence of shallot virus x (shvx), a new virus isolated from shallot, has been determined. the sequence contains six open reading frames (orfs) which encode putative proteins (in the 5' to 3' direction) of m(r) 194528 (orf1), 26333 (orf2), 11245 (orf3), 42209 (orf4), 28486 (orf5) and 14741 (orf6). the orf1 protein was found to be highly homologous to the putative potexvirus rna replicases; orf2, -3, -5 and -6 proteins also have analogues among the potex- and/or carlaviru ... | 1992 | 1339468 |
| [42k protein of shallot x virus is expressed in infected allium species plants]. | the main difference between genome structures of shallot virus x (shvx) and related potex- and carlaviruses is the unique gene of shvx coding for a 42k protein. the amino acid sequence of this protein was analyzed and compared with those of similar proteins from several newly characterised viruses of garlic. using antibodies against the recombinant 42k protein, expression of the 42k protein of shvx was detected in most of plants where the shvx coat protein is present. | 1995 | 7723759 |
| molecular characterization of the garlic virus x genome. | the complete nucleotide sequence of the cdna genome for garlic virus x (gvx), one of the major viruses infecting garlic plants, was determined. gvx is a single-stranded positive-sense rna virus consisting of 8106 nucleotides excluding the 3'-end poly(a) tail and contains six open reading frames (orfs) which encode putative proteins of 174 kda (orf1), 26 kda (orf2), 12 kda (orf3), 32 kda (orf4), 26 kda (orf5) and 15 kda (orf6). the putative viral proteins show similarity to those of carlaviruses ... | 1998 | 9460937 |
| identification of one of the major viruses infecting garlic plants, garlic virus x. | a partial cdna clone for garlic virus x (gvx) was isolated. gvx was identified immunologically with an antibody raised against the recombinant coat protein (cp) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic or streak symptoms. gvx belongs to an unassigned group of shvx and garv-type viruses rather than to carlaviruses or potexviruses. the recombinant cp of gvx was purified by ni(2+)-nta affinity chromatography. anti-gvx cp antibody was raised against the ... | 1997 | 9509408 |
| [amino acid sequences of proteins, coded by the 3'-ends of regions of genomes of two morphological classes of shallot virus x virions]. | | 1998 | 9929889 |
| complete nucleotide sequences of garlic viruses a and c, members of the newly ratified genus allexivirus. | complete genomic sequences of garlic viruses a (gar v-a) and c (gar v-c), members of an unassigned virus group recently identified in garlic plants, were determined. their respective genomes consist of 8 660 and 8 405 nucleotides. the genomic structure and organization of these viruses are similar to shallot virus x (shvx) which is the type species of the newly ratified genus allexivirus. phylogenetic analysis based on the amino acid sequences of the putative proteins including rna-dependent rna ... | 1999 | 10542028 |
| detection of infectious viral particles in plant protoplasts inoculated with transcripts of full-length shallot virus x cdna. | flexible filamentous shallot virus x (shvx) particles were detected in extracts of beta vulgaris protoplasts inoculated with transcripts from a full-length shvx cdna. extracts from shvx-infected protoplast were infectious for shvx-healthy shallot seedlings. western blot analysis of inoculated plants revealed the accumulation of the shvx coat protein, while electron microscopy confirmed the presence of shvx virions. the results suggest that the in vitro rna transcripts from full-length shvx cdna ... | 2001 | 11504426 |
| [the 42k protein of the shallot virus x participates in formation of the viral particles]. | | 2002 | 12500548 |
| detection and classification of allexiviruses from garlic in china. | degenerate primers for rt-pcr were designed and used to amplify genome fragments ( c. 750 nt in the coat protein-orf6 region) of allexiviruses from a total of 28 garlic samples from 24 provinces in china. many samples contained more than one distinct sequence. a total of 60 different sequences were obtained. phylogenetic analysis and two-way comparisons were used to assess the status of the sequences and to re-examine the criteria for distinguishing species within the genus. most of the sequence ... | 2004 | 14991435 |
| sequence comparison and phylogeny of nucleotide sequence of coat protein and nucleic acid binding protein of a distinct isolate of shallot virus x from india. | shallot virus x (shvx), a type species in the genus allexivirus of the family alfaflexiviridae has been associated with shallot plants in india and other shallot growing countries like russia, germany, netherland, and new zealand. coat protein (cp) and nucleic acid binding protein (nb) region of the virus was obtained by reverse transcriptase polymerase chain reaction from scales leaves of shallot bulbs. the partial cdna contained two open reading frames encoding proteins of molecular weights of ... | 2011 | 23637504 |
| [persistent shallot virus x infection correlates with transcriptional repression of plant cell rna-dependent rna polymerase and dcl proteins in plant roots]. | shallot virus x is a typical representative of allexiviruses. the transcription levels of principal genes involved in the rna silencing in healthy and shallot virus x-infected plants have been quantified by real-time polymerase chain reaction. there is a negative correlation between the reproduction rates of rna virus and the levels of rna-dependent rna polymerase and dcl proteins in roots and leaves of infected plants. these observations indicate that shallot x virus employs noncanonical ways o ... | 2017 | 28251975 |
| translation of the shallot virus x tgb3 gene depends on non-aug initiation and leaky scanning. | triple gene block (tgb), a conserved gene module found in the genomes of many filamentous and rod-shaped plant viruses, encodes three proteins, tgb1, tgb2 and tgb3, required for viral cell-to-cell movement through plasmodesmata and systemic transport via the phloem. the genome of shallot virus x, the type species of the genus allexivirus, includes tgb1 and tgb2 genes, but contains no canonical orf for tgb3 protein. however, a tgb3-like protein-encoding sequence lacking an aug initiator codon has ... | 2015 | 26296665 |
| accumulation of changes in the genome of shallot virus x persisting in vegetatively reproduced plants. | | 2013 | 24150581 |