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nomenclature and relationships of some brazilian leguminous potyviruses related to bean common mosaic and/or passionfruit woodiness viruses.the main brazilian literature of the last 10 years on potyviruses of leguminous plants related to bean common mosaic virus (bcmv) and/or to passionfruit woodiness virus (pwv) is discussed and summarized. the viruses dealt with are canavalia acronecrosis, mosaico de canavalia, cassia yellow spot, cowpea green vein-banding, cowpea rugose mosaic and cowpea severe mottle. the viruses have similar biological properties, such as a host range restricted mainly to the leguminosae, aphid transmission, se ...19921450754
coat protein of potyviruses. 7. amino acid sequence of peanut stripe virus.the amino acid sequence of the 287-residue coat protein of peanut stripe virus (pstv) was determined from the sequences of overlapping peptide fragments. results indicated that the amino terminus was blocked by an acetyl group, as has previously been found for the coat protein of johnsongrass mosaic potyvirus. comparison of the pstv sequence with coat proteins of 20 distinct potyviruses gave sequence identities of 47-57%, except for zucchini yellow mosaic virus (zymv), passionfruit woodiness vir ...19911863222
coat protein of potyviruses. 5. symptomatology, serology, and coat protein sequences of three strains of passionfruit woodiness virus.three strains of passionfruit woodiness virus, tip blight (pwv-tb), severe (pwv-s) and mild (pwv-m), were compared on the basis of their biological, serological and coat protein structural properties. each of the strains could be distinguished on the basis of their reactions on selected test plant species but no differences were observed in the serological properties of the three pwv strains. molecular weight estimates on sds-page suggest the pwv coat protein contains 275 amino acid residues and ...19883202695
cloning, sequencing, and expression in escherichia coli of the coat protein gene of a new potyvirus infection south african passiflora.complementary dna representing 1418 nucleotides (nt) of the 3'-poly(a)-proximal tract of the genomic rna of a potyvirus causing woodiness disease in south african passion fruit, was cloned and sequenced. the sequence contained a single long open reading frame (orf) of 1188 nt with no initiation codon, and a 3'-non-coding region (3'-ncr) of 230 nt followed by a poly-adenylate tract. comparison of the orf with other potyviral coat protein (cp) sequences led to the prediction that a 279 residue cp ...19937916588
cowpea aphid borne mosaic virus-morocco and south african passiflora virus are strains of the same potyvirus.high performance liquid chromatography (hplc) profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphid-borne mosaic virus (cabmv). some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus (b1cmv) while other reports have concluded that this relationship is distant. in this report a tryptic digest of the coat protein of cabmv-morocco was compared w ...19948002788
unusual amino-terminal sequence repeat characterizes the capsid protein of dasheen mosaic potyvirus.the 3'-terminal region of a florida isolate of dasheen mosaic potyvirus (dmv-la) genome including the coat protein (cp) gene was cloned and sequenced. protease digestion was predicted to occur between the glutamine and alanine residues at positions 79 and 80 of the 408 residue long polypeptide to produce a cp of 329 amino acids with an estimated m(r) of 36,229. following the putative protease recognition site is a dag sequence, which is conserved among aphid-transmitted potyviral cps. there is a ...19948113735
hepatitis c virus seropositivity, but not hepatitis b virus carrier or seropositivity, associated with increased pulse wave velocity.immune mechanisms have important roles in the pathogenesis of atherosclerosis. the present study was conducted to evaluate the association of pulse wave velocity (pwv) with either hepatitis c or b. in 7514 subjects, we determined the pwv, as well as the plasma levels of hepatitis b virus antigen and antibody and of hepatitis c virus antibody. the results of multiple regression analysis showed that seropositivity for hepatitis c virus was a significant variable for pwv independent from atheroscle ...200312535755
a study of arteriosclerosis in healthy subjects with hbv and hcv infection.it is unclear whether infection with hepatitis b virus (hbv) or hepatitis c virus (hcv) affects arteriosclerosis. we performed a cross-sectional study to clarify the effect of hbv and hcv infection on arteriosclerosis.200516322949
the potyvirus associated with the dappled fruit of passiflora edulis in kagoshima prefecture, japan is the third strain of the proposed new species east asian passiflora virus (eapv) phylogenetically distinguished from strains of passion fruit woodiness virus.a potyvirus (isolate ib) causing dappled or faded fruits and foliar mosaic symptoms of purple passionfruit, was found in the botanical garden of kagoshima university, japan. this isolate--differed in host range from isolates of passion fruit woodiness virus (pwv)-ao, previously reported to cause "woodiness" in japan. isolates ib and ao had 83% amino acid identity in their coat proteins (cps). in phylogenetic analysis, east asian isolates ib, ao, and pwv-taiwan clustered together, and were distin ...200616328141
functional and structural markers of atherosclerosis in human immunodeficiency virus-infected patients.we investigated functional and structural markers of atherosclerosis in human immunodeficiency virus (hiv)-infected patients in relation to the presence of the metabolic syndrome (ms).200616545639
cowpea aphid-borne mosaic virus (cabmv) is widespread in passionfruit in brazil and causes passionfruit woodiness disease.leaf samples of yellow passionfruit (passiflora edulis f. flavicarpa) displaying fruit woodiness symptoms were collected in seven brazilian states and the federal district. viral infection was confirmed by host range and elisa, and fourteen viral isolates were obtained. all isolates were capable of infecting several leguminous host species, although differences in symptom severity were noticeable. woodiness symptoms were reproduced in yellow passionfruit, and mosaic symptoms were induced in comm ...200616596328
aortic stiffness in untreated adult patients with human immunodeficiency virus infection.hiv infection is associated with chronic immune activation, subclinical inflammation, and an atherogenic metabolic profile. it remains controversial whether hiv infection is a risk factor for accelerated arteriosclerosis independent from the effects of antiretroviral drugs. we investigated whether aortic stiffness, an early marker of arteriosclerosis, is increased in hiv patients who were not under antiretroviral treatment. in 39 untreated hiv-infected patients and 78 individually matched age-, ...200818559718
hepatitis c virus infection as a risk factor for increased aortic stiffness and cardiovascular events in dialysis patients.although links have been found between microorganisms and cardiovascular diseases, the role of hepatitis c virus (hcv) infection in the pathogenesis of arteriosclerosis and cardiovascular events is unclear. the primary objective of this research was to examine whether hcv infection is associated with increased aortic stiffness and cardiovascular events in chronic hemodialysis patients.200818587722
towards a system for the identification and classification of potyviruses. ii. virus particle length, symptomatology, and cytopathology of six distinct viruses.the mean particle lengths of lettuce mosaic virus (lmv), bean common mosaic virus (bcmv), passionfruit woodiness virus (pwv), potato virus y (pvy), sugarcane mosaic virus (scmv), and one strain of bean yellow mosaic virus (bymv) were all shown to be close to 750 nm. however, the length of two other serologically closely related strains of bymv, variants of pea mosaic virus referred to as pmv and spmv, had significantly longer particles, about 792 and 842 nm. each of the distinct viruses had read ...198118635068
helicobacter pylori infection and arterial stiffness in patients with type 2 diabetes mellitus.epidemiologic studies have suggested possible atherogenic roles for such pathogens as chlamydia pneumoniae, helicobacter pylori (hp), cytomegalovirus, and herpes simplex virus. the aim of the present study was to examine the relationship between seropositivity of antibodies to hp (hp infection) and arterial stiffness determined by pulse wave velocity (pwv) in 130 patients (73 men and 57 women) with type 2 diabetes mellitus without a history of cardiovascular disease. the prevalence of hp infecti ...200819013302
detection of a resistance gradient to passion fruit woodiness virus and selection of 'yellow' passion fruit plants under field conditions.productivity of 'yellow' passion fruit (passiflora edulis sims. f. flavicarpa o. deg.) is reduced by infection with cowpea aphid-borne mosaic virus (cabmv). we examined resistance in 72 yellow passion fruit plants grown from open-pollinated commercial seed. plants were mechanically inoculated with cabmv virus and maintained in the field in order to select contrasting genotypes for resistance. isolates were obtained from symptomatic leaves of yellow passion fruit plants from field production in l ...200819048500
association of highly active antiretroviral therapy with increased arterial stiffness in patients infected with human immunodeficiency virus.metabolic disorders associated with atherosclerosis and cardiovascular disease have been described in "hiv-infected" individuals. we investigated (i) whether normotensive "hiv-infected" individuals and hypertensive patients have similarities regarding their arterial elastic properties and (ii) the effect of highly active antiretroviral therapy (haart) and metabolic factors on arterial stiffness.200919556973
increased arterial stiffness in hiv-infected children: risk factors and antiretroviral therapy.recent evidence suggests that both the hiv virus and antiretroviral therapy (art) are associated with premature atherosclerosis in adults. increased arterial stiffness as assessed by pulse wave velocity (pwv) has been associated with adverse cardiovascular outcome in adults. the relationship between hiv infection and treatment and arterial stiffness has not been evaluated in children.200920032537
indigenous and introduced potyviruses of legumes and passiflora spp. from australia: biological properties and comparison of coat protein nucleotide sequences.five australian potyviruses, passion fruit woodiness virus (pwv), passiflora mosaic virus (pamv), passiflora virus y, clitoria chlorosis virus (clcv) and hardenbergia mosaic virus (harmv), and two introduced potyviruses, bean common mosaic virus (bcmv) and cowpea aphid-borne mosaic virus (cabmv), were detected in nine wild or cultivated passiflora and legume species growing in tropical, subtropical or mediterranean climatic regions of western australia. when clcv (1), pamv (1), pavy (8) and pwv ...201121744001
premature atherosclerosis in patients with acquired immunodeficiency syndrome.increased risk of atherosclerosis has been reported in patients with human immunodeficiency virus (hiv) infection since highly active antiretroviral therapy (haart) has come into use. however, there is no clear evidence of premature atherosclerosis in chinese hiv-infected patients. our study was designed to determine the relationship between hiv infection and atherosclerosis in chinese hiv-infected patients.201022166520
inheritance of resistance to potyviruses in phaseolus vulgaris l. iii. cosegregation of phenotypically similar dominant responses to nine potyviruses.we have identified monogenic dominant resistance to azuki bean mosaic poty virus (azmv), passionfruit woodiness potyvirus-k (pwv-k), zucchini yellow mosaic potyvirus (zymv), and a dominant factor that conditioned lethal necrosis to thailand passiflora potyvirus (thpv), in phaseolus vulgaris 'black turtle soup 1'. resistance to azmv, pwv-k, zymv, watermelon mosaic potyvirus, cowpea aphid-borne mosaic potyvirus, blackeye cowpea mosaic potyvirus, and lethal necrosis to soybean mosaic potyvirus and ...199424178089
is the atherosclerotic process accentuated under conditions of hiv infection, antiretroviral therapy, and protease inhibitor exposure? meta-analysis of the markers of arterial structure and function.to evaluate the apparent association of human immunodeficiency virus (hiv) infection, antiretroviral therapy (art), and protease inhibitor (pi) exposure with the functional and structural markers of vasculature.201526188532
endothelial activation and cardiometabolic profiles of treated and never-treated hiv infected africans.the role the human immunodeficiency virus (hiv) and antiretroviral treatment on endothelial activation, and the subsequent relationship with cardiovascular disease, is not well understood. we investigated endothelial activation, inflammatory and cardiometabolic profiles, and measures of vascular structure and function of 66 antiretroviral treated (art), 78 never-treated (no-art) hiv infected and 165 hiv free africans.201525795556
hiv is an independent predictor of aortic stiffness.patients with treated human immunodeficiency virus-1 (hiv) infection are at increased risk of cardiovascular events. traditionally much of this risk has been attributed to metabolic and anthropometric abnormalities associated with hiv, which are similar to the metabolic syndrome (ms), an established risk factor for cardiovascular mortality. it remains unclear whether treated hiv infection is itself associated with increased risk, via increase vascular stiffness.201425187084
metabolic syndrome in human immunodeficiency virus-positive subjects: prevalence, phenotype, and related alterations in arterial structure and function.human immunodeficiency virus (hiv) infection itself and highly active antiretroviral treatment (haart) have been proposed to be associated with a higher prevalence of metabolic syndrome, but, to date, prevalence and phenotype of metabolic syndrome among hiv subjects and the related structural and functional vascular alterations are not conclusively defined.201323885973
potent antiretroviral therapy for human immunodeficiency virus infection increases aortic stiffness.highly active antiretroviral therapy for aids is known to increase cardiovascular risk, but the effects of potent antiretroviral agents according to gender are unknown.201223197168
protease inhibitor treatment effect on aortic stiffness in normotensive patients with human immunodeficiency virus infection.human immunodeficiency virus (hiv) infection and protease inhibitor (pi)-based antiretroviral treatment might increase large artery (aortic) stiffness compared with healthy untreated controls. to clarify the role of pi therapy in the progression of subclinical arteriosclerosis in patients with hiv, we investigated the impact of pi treatment on arterial stiffness.201222698629
correlation between abnormal pap smear finding and brachial-ankle pulse wave velocity in korean women.cervical cancer is caused by chronic human papilloma virus (hpv) infection. pap smear is very efficient examination for early detecting cervical cancer. inflammation reaction due to chronic infection is one of the major causes of atherosclerosis. pulse wave velocity (pwv) is commonly used in predicting subclinical atherosclerosis. but no study was done about correlation between cervical cancer and pwv.201326064840
virus removal vs. subsurface water velocity during slow sand filtration.in an attempt to obtain a conservative estimate of virus removal during slow sand and river bank filtration, a somatic phage was isolated with slow decay and poor adsorption to coarse sand. we continuously fed a phage suspension to a 7-m infiltration path and measured the phage removal. in a second set of experiments, we fed the phage suspension to 1-m long columns run at different pore water velocities. using the data obtained, a mathematical model was constructed describing removal vs. pore wa ...201526042970
complete nucleotide sequence of a new isolate of passion fruit woodiness virus from western australia.we determined the complete genome sequence of the passion fruit woodiness virus gld-1 isolate (pwv-gld-1) from australia and compared it with that of pwv-mu-2, another australian isolate of pwv. the genomes shared high sequence identity in both the complete nucleotide sequence and the orf amino acid sequence. all of the cleavage sites of each protein were identical to those of mu-2, and the sequence identity for the individual proteins ranged from 97.2 % to 100.0 %. however, the 5' untranslated ...201323508550
evaluation of the antioxidant activity of passion fruit (passiflora edulis and passiflora alata) extracts on stimulated neutrophils and myeloperoxidase activity assays.the antioxidant activity of methanol extracts from passiflora edulis and passiflora alata pulp, and p. edulis rinds, healthy or infected with the passion fruit woodiness virus (pwv), was investigated using the oxidant activities of the neutrophil and the neutrophil granule enzyme myeloperoxidase (mpo), both playing key roles in inflammation. the reactive oxygen species produced by stimulated neutrophils were evaluated by lucigenin-enhanced chemiluminescence (cl) and the activity of purified mpo ...201125212130
the complete genome sequence of a passion fruit woodiness virus isolate from australia determined using deep sequencing, and its relationship to other potyviruses.the complete genome sequence (9,858 nucleotides) of the passion fruit woodiness virus isolate mu-2 was determined using illumina sequencing. the large open reading frame (orf) encodes a polyprotein containing 3,086 amino acids, with an aug start codon and uaa stop codon. the polyprotein yielded 11 proteins (p1, hc-pro, p3, pipo, 6k1, ci, 6k2, nia-vpg, nia-pro, nib and cp). putative cleavage sites between them were identified by sequence comparison to those of other known potyviruses. accuracy of ...201121076846
[transmission of passion fruit woodiness virus by aphis gossypii (glover) (hemiptera: aphididae) and colonization of passion flower by the vector].the transmission of passion fruit woodiness virus (pwv) by aphis gossypii (glover) was evaluated. in two independent experiments, a. gossypii transmitted pwv to passion fruit plants at the rates of 75% and 100%, when eight and twelve viruliferous aphids were deposited by plant, respectively. at the end of the tests, nymphs of a. gossypii were observed in some of the passion fruit plants, suggesting that the aphid species was colonizing the plants. this seems to be the first report of passiflora ...200717352080
major sequence variations in the n-terminal region of the capsid protein of a severe strain of passionfruit woodiness potyvirus.the deduced coat protein sequence of the k strain of passionfruit woodiness virus differed significantly, particularly in the n terminus, from the sequences of the tb, m, and s strains of the virus. an antiserum that reacted strongly with the tb, m, and s strains reacted very poorly with the k strain.19921605743
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