Publications

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sequence analysis of cdna for the vp6 protein of group a avian rotavirus: a comparison with group a mammalian rotaviruses.cdna corresponding to the genomic segment 6 of avian rotavirus strain po-13, which has group a common and subgroup i antigens, but does not hybridize in northern blots with rna probes from group a mammalian rotaviruses, was cloned and sequenced. when the deduced amino acid sequence was compared between strain po-13 and eight group a mammalian rotaviruses, the extent of homology ranged from 73-75%. an alignment of the amino acid sequences allowed us to identify three amino acids (positions 120, 3 ...19957733831
prevalence of histopathological intestinal lesions and enteric pathogens in dutch commercial broilers with time.intestinal disease has a major impact on the broiler industry due to economic and welfare reasons. intestinal disease might occur due to a large number of reasons varying from well-defined pathogens to non-specific enteritis and complex syndromes. however, knowledge about the nature of intestinal disease and presence of enteric viruses in the dutch broiler industry is largely absent. therefore, a large-scale field study, in which 98 broiler flocks from 86 farms were sampled weekly, was started t ...201727557362
analysis of rotavirus species diversity and evolution including the newly determined full-length genome sequences of rotavirus f and g.rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. eight different rotavirus species (a-h) have been defined based on antigenicity and nucleotide sequence identities of the vp6 gene. here, the first complete genome sequences of rotavirus f (strain 03v0568) and g (strain 03v0567) with lengths of 18,341 and 18,186bp, respectively, are described. both viruses have open reading frames for rotavirus proteins vp1 to vp7 and nsp1 to nsp5 located at the 11 genome segme ...201323237956
sensitive sybr green-real time pcr for the detection and quantitation of avian rotavirus a.avian rotavirus a (artv-a) is a virus that affects young birds, causing acute diarrhea and economic losses in the poultry industry worldwide. the techniques used for the diagnosis of artv-a include electron microscopy, isolation in cell culture, and serology, as well as molecular techniques, such as the reverse transcription-polymerase chain reaction (rt-pcr). the objective of this work was to standardize a real-time rt-polymerase chain reaction (rt-qpcr) using sybr green chemistry for the rapid ...201830597964
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