| clinical and immunologic responses in patients with viral keratoconjunctivitis. | of 120 individuals suffering from follicular conjunctivitis, with or without keratitis, 56 had epidemic keratoconjunctivitis caused by adenovirus type 8. the remaining 64 patients had keratoconjunctivitis produced by several different viruses, including herpes simplex, adenoviruses types 3, 7, 8, 16, 21, and 29, and others. positive viral cultures were difficult to obtain after the first week of illness in most viral infections. serologic tests were far more successful in indetifying causative a ... | 1975 | 170831 |
| clinical and laboratory evaluation of epidemic keratoconjunctivitis due to adenovirus types 8 and 19. | during a community outbreak of epidemic keratoconjunctivitis due to adenovirus types 8 and 19, we compared the clinical and laboratory characteristics of the two viruses. much of the disease was mild and the keratoconjunctivitis associated with adenovirus type 8 was indistinguishable clinically from that associated with adenovirus type 19. adenovirus type 8 was isolated only from the conjunctival sac, whereas adenovirus type 19 was frequently cultured from the nose, throat, and conjunctiva. the ... | 1976 | 175659 |
| an outbreak of epidemic keratoconjunctivitis due to adenovirus type 8 in a babies home. | between october and december, 1973, an outbreak of epidemic keratoconjunctivitis occurred in a babies home in sapporo. 28 out of 58 infants and children suffered from the disease. infection seemed to have been transmitted within a room by hands of nursing staff with spread into other rooms through the dispensary. clinical manifestation in almost all of the patients was pseudomembraneous conjunctivitis without apparent corneal opacities. none of them showed systemic symptoms. virus isolation was ... | 1976 | 181868 |
| simultaneous nosocomial and community outbreak of epidemic keratoconjunctivitis with types 8 and 19 adenovirus. | in a simultaneous community and nosocomial (hospital-based) epidemic of keratoconjunctivitis lasting one year, 97 patients had clinical findings characteristic of ekc. seventy-one percent of the cases were confirmed by laboratory tests including viral isolation, antibody response detected by complement fixation and hemagglutination inhibition tests, or immunofluorescent detection of adenovirus antigen in conjunctival scrapings. the community epidemic was a mixed adenovirus type 19 and type 8 out ... | 1976 | 190749 |
| epidemic keratoconjunctivitis and chronic papillary conjunctivitis in london due to adenovirus type 19. | since july 1973 cases of keratoconjunctivitis resembling epidemic keratoconjunctivitis were observed in the external eye disease clinic at moorfields eye hospital; city road, london. adenovirus type 19 was isolated in human embryonic kidney cells from 21 patients. the majority were males between 20 and 40 years old. a small hospital outbreak involving six patients occurred. clinical features of the disease, consisting of moderate to severe follicular conjunctivitis, major subepithelial punctate ... | 1977 | 191054 |
| a mixed outbreak of epidemic keratoconjunctivitis due to adenoviruses types 29 and 8. | an outbreak of epidemic keratoconjunctivitis (ekc) occurred in northern bohemia in the summer of 1975. one hundred and four patients were treated. four strains of adenovirus type 29 with low or strong pronounced cross-neutralization against anti-ad 15 reference serum, and 2 adenovirus type 8 were isolated of 4 patients. type 8 strains were only obtained from conjunctiva, type 29 also from stools. from one patient, type 8 from conjunctiva and type 29 from stool were isolated simultaneously. out o ... | 1977 | 202111 |
| animal corneas as tools for the testing of antiviral compounds. | as a number of viruses can be titrated on corneas of various animals, the corneal method was used to detect antiviral activity of some derivatives of thiazolidine and related compounds with a low toxicity. in herpetic infections of rabbit cornea, 2-(1-isopropylidene)azino-3-(beta-d-ribofuranosyl)-methoxycarbonylmethylenethiazolidine-4-one showed for a mean of 2.1 greater activity than idu. in piglet corneas, lesions produced by adenovirus type 8 were inhibited by a mean of 3.4 log cid50 by 5-(2, ... | 1977 | 280135 |
| compound purification and comparison of adenovirus hexons. | hexon antigens were separated from the cell-free supernatant of type 1 and type 8 adenovirus-infected cells by ammonium sulfate precipitation and purified by anion exchange chromatography, immune absorption, and exclusion chromatography. the antigens were compared with purified soluble hexons extracted from cells infected with the same adenovirus types. the procedure was found to be suitable for the preparation of pure, homogeneous type 1 hexons from the supernatant, but not for that of adenovir ... | 1976 | 941401 |
| epidemic keratoconjunctivitis: a community outbreak of mixed adenovirus type 8 and type 19 infection. | during the fall and winter of 1973, 145 cases of epidemic keratoconjunctivitis were diagnosed in nashville, tennessee. of the 74 cases studied virologically and/or serologically, 62% were caused by adenovirus type 8, and 28% were caused by adenovirus 19. whereas adenovirus type 8 was isolated only from conjunctival scrapings, adenovirus type 19 was isolated from nose and throat swabs as well as from conjunctivae. the two viruses produced clinically indistinguishable eye disease and household sec ... | 1975 | 1159321 |
| an experimental animal model of adenovirus-induced ocular disease. the cotton rat. | the adenoviruses are a common cause of eye disease in humans and clinically cause three basic syndromes: epidemic keratoconjunctivitis, pharyngo-conjunctival fever, and nonspecific follicular conjunctivitis. although many serotypes of the adenovirus have been implicated, types 8, 19, and 37 are associated most commonly with ocular disease. there has not been a well-defined and reproducible animal model of this disease. the eyes of cotton rats inoculated with either adenovirus type 5 or type 8 de ... | 1992 | 1323254 |
| description of an adenovirus type 8 outbreak in hospitalized neonates born prematurely. | an adenovirus (ad) type 8 outbreak was prospectively studied in a neonatal intensive care unit. nasopharyngeal secretions were cultured weekly for viruses and clinical information was obtained daily in each infant. eleven of 112 neonates were infected with ad; 8 of 9 available isolates represented one variant of ad 8. the median age at infection was 54 days and the median duration of virus shedding was 2 days. seven of 11 infants had onset of new symptoms and/or required acute respiratory suppor ... | 1992 | 1608683 |
| [epidemic keratoconjunctivitis: treatment results during an epidemic]. | 150 patients with adenovirus type 8 infection where treated at random, in a prospective study. based upon a quantifiable conjunctivitis severity score we tried to find out where there are differences in treatment. the best results were seen using polyvinylpyrrolidone-iodine (betaisodona) although it could not prevent totally subepithelial corneal infiltrates. the combination of exogenous interferon alpha with polyvinylpyrrolidone-iodo-drops or trifluorothymidine-drops was less successful. we cou ... | 1990 | 1701507 |
| a community outbreak of epidemic keratoconjunctivitis in central australia due to adenovirus type 8. | a large outbreak of epidemic keratoconjunctivitis occurred in alice springs, australia, between july and september 1989. initially, there was a large outbreak within two child care centers, followed by a more prolonged outbreak within the community. a simultaneous outbreak occurred among staff at the local hospital. infected children developed mild conjunctivitis, whereas adults developed typical keratoconjunctivitis. conjunctival swabs from 30 cases were cultured and adenovirus was recovered fr ... | 1991 | 1955711 |
| keratoconjunctivitis due to adenovirus type 8: report on a large outbreak. | a large nosocomial, community-acquired outbreak of keratoconjunctivitis due to adenovirus type 8 at a large military teaching hospital is described. one hundred thirty-two cases were identified with initial case confirmation by viral isolation and subsequent case confirmation based on clinical findings. infection usually presented as a severe keratoconjunctivitis with 35% of infected patients developing subepithelial corneal infiltrates. most infections were acquired by hand-to-eye contact or by ... | 1991 | 2029116 |
| restriction site mapping of adenovirus type 8 genome types. | the dna of 60 adenovirus type 8 (av8) isolates (collected during the period 1961 to 1982, mostly in western germany) was analysed by 6 endonucleases and revealed 6 different genome types, thus implying that the variability of av8 is relatively low. it was found that 45 isolates belonged to the genome type d1. restriction site maps of a prototype d1 and of all deviating restriction variants were elaborated for enzymes bamhi, bglii, hind-iii and sali. | 1990 | 2087599 |
| keratoconjunctivitis due to adenovirus type 8: a local outbreak. | an outbreak of acute keratoconjunctivitis occurred in 24 patients who had visited a private ophthalmology office between july and october 1986. the median incubation period was 12.6 days (range seven to 21 days). adenovirus type 8 was isolated from three of the five patients in whom cultures were done. this outbreak illustrates the highly contagious nature of adenovirus infection and the potential for transmission during routine office visits. | 1989 | 2536959 |
| [epidemic keratoconjunctivitis. laboratory study of patients infected with adenovirus type 8]. | the authors tried to detect, using different methods, the causal agent of the disease in eight patients with epidemic keratoconjunctivitis. from the conjunctival sacs of one ambulatory patient and six of seven patients hospitalized on account of other eye diseases they isolated 11 strains of adenovirus type 8 on cell cultures from human embryonic lungs. using direct and indirect elisa, they detected adenovirus antigen in four of seven examined smears from the conjunctival sac without previous cu ... | 1989 | 2551515 |
| a large outbreak of epidemic keratoconjunctivitis: problems in controlling nosocomial spread. | between july 1985 and january 1986, 401 patients with adenovirus epidemic keratoconjunctivitis (ekc) were seen at the illinois eye and ear infirmary. of the cases, 110 (27%) were nosocomial; the other 291 patients had community acquired infection. the highest attack rates of ekc occurred in patients attending specialty clinics; the overall attack rate among clinic patients was 4.7/1,000 clinic visits. all nosocomial cases were caused by adenovirus type 8; community acquired cases were a mixture ... | 1989 | 2555421 |
| epidemic keratoconjunctivitis in a university medical center ophthalmology clinic; need for re-evaluation of the design and disinfection of instruments. | during the period from early december 1987 to late january 1988, an outbreak of epidemic keratoconjunctivitis (ekc) caused by adenovirus type 8 occurred at a university medical center eye clinic. a telephone survey of patients revealed an attack rate of 17% among patients seen during the epidemic period. a case-control study demonstrated that development of ekc was associated with exposure to pneumotonometry and to three caregivers. after controlling for exposure to pneumotonometry, however, onl ... | 1989 | 2614054 |
| histopathology of adenovirus type 8 keratitis. | | 1987 | 2824130 |
| epidemic keratoconjunctivitis. | following a definition of epidemic keratoconjunctivitis (ekc) and the etiologic agent responsible, a brief history of the disorder dating back to 1889 is given. the clinical course, signs, symptoms, and corneal involvement of this disease, which sets it apart from other adenovirus conjunctivitis, are described. during the late summer of 1981, epidemics of viral conjunctivitis were reported in india and miami, florida. at this same time, in our office in memphis tennessee, we examined and treated ... | 1988 | 2830832 |
| a molecular study of adenovirus type 8 isolated from viral conjunctivitis in the kaohsiung area during 1983-1984: a chronological comparison of subtypes by endonuclease cleavage analysis. | | 1987 | 2838647 |
| three adenovirus type 8 genome types defined by restriction enzyme analysis: prototype stability in geographically separated populations. | adenovirus type 8 strains were collected over a 19-year period from eye specimens from patients with keratoconjunctivitis. these strains were divided by restriction enzyme analysis with the endonucleases sali, hindiii, saci, kpni, and smai into three genotypic subgroups. the prototype strain (trim) was found throughout the united states from 1966 through 1985 and also in taiwan and greece in the early 1980s. genotype 8c was identified in an arc from maryland to missouri to alabama from 1971 thro ... | 1986 | 3007566 |
| adenovirus type 8 keratoconjunctivitis--an outbreak and its treatment with topical human fibroblast interferon. | an outbreak of keratoconjunctivitis is described which involved at least 186 people; adenovirus type 8 was identified in 50 of the cases. topical human fibroblast interferon was assessed in a double-blind, placebo-controlled study in which 34 patients participated. seventeen of the 34 trial patients yielded adenovirus type 8; three were infected with adenovirus type 7. the outbreak was curtailed by control of infection measures: principally careful hand-washing by medical personnel between cases ... | 1986 | 3016080 |
| the efficiency of various laboratory methods for the diagnosis of adenovirus conjunctivitis. | virological and serological data from 242 patients with conjunctivitis or keratoconjunctivitis, infected with adenovirus type 8, and of 109 patients infected with other adenovirus types were compared by means of an electronic data processing system. about 55% of the patients in both groups showed a diagnostic antibody rise in genus-specific (antihexon) complement-fixation. this rate is significantly lower than that obtained with genus-specific elisa igg or iga, or with neutralization tests again ... | 1987 | 3037827 |
| applanation tonometer tip sterilization for adenovirus type 8. | eighty-nine ophthalmologists in the dallas-fort worth area were surveyed to find the methods used to sterilize applanation tonometer tips. sixteen different methods were in use, with the most popular being alcohol wipes (26%) and diluted sodium hypochlorite soak (23%). six of the most frequently used sterilization techniques were evaluated for removal of type 8 adenovirus applied to sterile tonometer tips. adenovirus was removed or inactivated from applanation tonometer tip surfaces by using one ... | 1987 | 3431823 |
| [intranuclear changes of hela cells by adenovirus type 8 infection]. | | 1970 | 4195619 |
| [unusual outbreak of epidemic keratoconjunctivitis due to adenovirus type 8]. | | 1965 | 4287440 |
| [isolation and differeniation of adenovirus type 8 in epidemic keratoconjunctivitis in leipzig]. | | 1967 | 4296639 |
| [isolation of adenovirus type 8 in patients with epidemic keratocojunctivitis]. | | 1968 | 4301279 |
| adenovirus type 8 keratoconjunctivitis in the united states. 3. epidemiologic, clinical, and microbiologic features. | | 1970 | 4313971 |
| rescue of heat-inactivated adenovirus type 1 and 6 by ultraviolet-irradiated adenovirus type 8. | | 1970 | 4316777 |
| [nuclear changes of hela cells induced by adenovirus type 8 infection]. | | 1970 | 4317519 |
| serologic evidence that infectious canine hepatitis virus commonly infects humans and is related to human adenovirus type 8. | | 1970 | 4319375 |
| adenovirus type 8-associated follicular conjunctivitis and keratoconjunctivitis in patients subjected to tonometry. i. virological findings. | | 1969 | 4329064 |
| adenovirus type 8-associated keratoconjunctivitis: hospital infections and secondary spread in stockholm, 1967. | | 1969 | 4329065 |
| adenovirus type 8 infections in the united states. iv. observations on the pathogenesis of lesions in severe eye disease. | | 1972 | 4335566 |
| outbreak in bristol of conjunctivitis caused by adenovirus type 8, and its epidemiology and control. | in an investigation of an outbreak of adenovirus type 8 infection involving over 40 people it was found that the infection spread initially within the eye hospital, but subsequently involved several family contacts and two local hospitals for mentally subnormal patients. presumptive diagnosis should be possible before subepithelial opacities have developed provided an adequate history is taken, and had that been done in this outbreak it is reasonable to suppose that many cases could have been pr ... | 1973 | 4349336 |
| treatment of adenovirus type 8 kerato-conjunctivitis. | | 1972 | 4352134 |
| epidemic keratoconjunctivitis. a severe industrial outbreak due to adenovirus type 8. | | 1973 | 4356635 |
| epidemic keratoconjunctivitis in the west of scotland, 1967-72. | outbreaks of epidemic keratoconjunctivitis have occurred among workers in shipyards and other industrial concerns in the west of scotland in 1956, 1967 and 1971-72. in the most recent episode 220 persons were known to be affected and those mainly involved were shipyard personnel working on the open decks of ships under construction; only a few non-industrial workers were affected. as in previous outbreaks adenovirus type 8 was shown to be the causal organism. it is likely that spread of the viru ... | 1974 | 4529095 |
| [immunofluorescence in the diagnosis of epidemic keratoconjunctivitis. i. preparation of monotypic fluorescent antibody to adenovirus type 8]. | | 1968 | 4881803 |
| adenovirus type 8 antigens and interferon induction. | | 1969 | 4902268 |
| a survey of antibodies to adenovirus 8 and coxsackievirus a21 in human sera. | studies have recently been published of surveys of antibodies to common respiratory viruses in human sera from several parts of the world. the present article reports the findings of a survey of antibodies to two more viruses (adenovirus type 8 and coxsackievirus type a21) in human sera mainly collected from six widely separated geographical regions (alaska, england, marshall islands, sarawak, south-west africa and tunisia).a world-wide geographical distribution of infection with these two virus ... | 1966 | 5296130 |
| enhancement of the infectivity titer of adenovirus type 8. | | 1970 | 5437909 |
| soluble components of adenovirus type 8. | soluble components of type 8 adenovirus have been studied. four different components were isolated by anion-exchange chromatography and purified by further chromatographic procedures, by zonal centrifugation, and by erythrocyte absorption and elution. the four components exhibited the following characteristics. (i) fiber antigen was trypsin-resistant and functioned as incomplete hemagglutinin (agglutinated rat and human erythrocytes only in the presence of certain types of adenovirus antisera). ... | 1970 | 5497886 |
| a large outbreak of keratoconjunctivitis due to adenovirus type 8. | a large nosocomial outbreak of keratoconjunctivitis due to adenovirus type 8 is described. two hundred cases were identified, 123 by isolation of the virus and 77 by detecting hi antibodies in convalescent sera. infection usually presented as a severe keratoconjunctivitis, and 107 (54%) of infected patients developed sub-epithelial corneal opacities. the majority (66%) of infections were acquired at the accident and emergency department attached to a large urban eye hospital when patients attend ... | 1984 | 6094666 |
| molecular epidemiology of adenovirus type 8 (ad 8) in taiwan: four subtypes recovered during the period of 1980-1981 from patients with epidemic keratoconjunctivitis. | adenovirus type 8 (ad 8) has been the major and important causative agent of epidemic keratoconjunctivitis (ekc). by enzymatic cleavage analysis with five endonucleases, psti, hindiii, bamhi, sali and ssti, 27 out of 149 ad 8 isolates recovered from patients with ekc during the period from july, 1980 to july, 1981 in kaohsiung, taiwan, were studied. by cleavage patterns, 27 ad 8 isolates in kaohsiung were classified into four subtypes which were found to be different from the subtypes (ad 8a, ad ... | 1984 | 6098752 |
| restriction site maps of the human adenovirus type 8 dna. | restriction site maps of human adenovirus 8 (ad h 8) were constructed with bamhi, hindiii, psti, sali and kpni endonucleases. the genome size was found to be 22.1 to 22.3 x 10(6) mr. comparison of the results with the data available on h ad subgenera a, b, c showed that the sali enzyme revealed subgenus-specific differences in the genomes. similar patterns of the sali fragments in both type 8 and 10 suggest that the differences were specific for the subgenus d of h ad. | 1983 | 6138993 |
| [the effects of topical steroids in epidemic kerato-conjunctivitis (author's transl)]. | fifty-three patients with proven adenovirus type 8 infection were treated at random, in a prospective study, with either artificial tears or topical steroids over six weeks. severe cases of nummular keratitis, as well as deep keratitis and slight iritis, were significantly less frequent under steroids. no difference existed with respect to the course of the conjunctivitis and the frequency of a superficial punctate keratitis. furthermore, the overall frequency of nummular keratitis was equal in ... | 1980 | 6256579 |
| epidemic keratoconjunctivitis caused by adenovirus type 8: epidemiologic and laboratory aspects of a large outbreak. | during late 1977 and early 1978, 192 cases of epidemic keratoconjunctivitis occurred in a community in central georgia. in 86 of these cases, the patient had been exposed to a single ophthalmologist's office (physician a) 3-22 days before onset of illness. patients considered as cases were more likely to have had invasive ophthalmologic procedures than non-cases (p < 0.001), and a linear relationship existed between the number of procedures performed and the attack rate (r = 0.91, p < 0.05). ade ... | 1981 | 6257110 |
| adenovirus eye infections in an australian city, 1972-9. | a number of adenovirus serotypes have been associated with both sporadic cases and outbreaks of conjunctivitis and pharyngoconjunctival fever but only adenovirus type 8 and adenovirus type 19 have been responsible for wide-spread epidemic kerato-conjunctivitis. in melbourne, australia, in the past eight years these two serotypes have been prevalent, resulting in an outbreak of adenovirus type 8 kerato-conjunctivitis in 1976-7 followed by adenovirus type 19 kerato-conjunctivitis in 1978-9. during ... | 1981 | 6257780 |
| [studies on experimental chemotherapy of epidemic keratoconjunctivitis due to human adenovirus type 8 (author's transl)]. | | 1980 | 6261556 |
| canine viruses and multiple sclerosis. | serum and cerebrospinal fluid from multiple sclerosis (ms) patients and control subjects were tested and compared for presence and titer of neutralizing antibody against the most common canine viruses. canine viruses included canine distemper virus (cdv), canine adenovirus 1 (cav-1), canine parainfluenza virus (cpiv), canine herpesvirus (chv), canine coronavirus (ccv), and canine parvovirus (cpv). neutralizing titers against measles virus (mv) and human adenovirus 8 (ha8) were also tested. signi ... | 1981 | 6267515 |
| human fibroblast interferon in treatment of viral diseases of the skin and mucous membranes. | the therapeutic effect of human fibroblast interferon (hfif) was studied in 33 patients. seven had ophthalmic lesions (three due to adenovirus type 8, four to herpes simplex virus, and one to herpes zoster) and were treated with hfif. in the four patients with herpes simplex keratitis, hfif was given in addition to standard therapy or together with other antiviral agents. ten other patients, 8 with herpes simplex or herpes zoster skin or mucous membrane lesions, were treated with hfif. hfif was ... | 1983 | 6319325 |
| restriction endonuclease cleavage analysis of adenovirus type 8: two new subtypes from patients with epidemic keratoconjunctivitis in sapporo, japan. | we studied the restriction endonuclease cleavage patterns of dnas of adenovirus type 8 (ad 8) isolated from epidemic keratoconjunctivitis cases. dnas of 25 ad 8 isolates collected during the period from 1975 to 1981 in sapporo were subjected to enzymatic cleavage with psti, bamhl , hindiii and sali. on the basis of the cleavage patterns, the isolates were divided into two subtypes, a and b, both different from trim strain used as the ad 8 prototype strain. subtype a was prevalent in the period f ... | 1983 | 6328068 |
| development and preliminary evaluation of an enzyme immunosorbent assay for the detection of adenovirus type 8. | epidemic keratoconjunctivitis (ekc) is a highly contagious adenoviral ocular infection which can occur in outbreaks and is predominantly caused by adenovirus type 8 (ad8). detection and typing of this virus after isolation is generally by serum neutralisation or more complex molecular techniques. these methods can be time consuming particularly during outbreaks. therefore, an ad8 specific antigen capture enzyme immunosorbent assay (eia) was developed as a rapid and cost effective diagnostic meth ... | 1994 | 7534818 |
| adenovirus cross-infection: a continuing problem. | nosocomial outbreaks of epidemics of adenovirus keratoconjunctivitis are frequently reported even though the simple measures to prevent or limit such occurrences are well documented. there have been two such outbreaks associated with the accident and emergency department (a&e) of a large, urban eye hospital in recent years. in the first--involving at least 200 cases--there was a delay of two months in initiation of control measures, with consequent potentiation and prolongation of the outbreak. ... | 1995 | 7560960 |
| restriction endonuclease analysis of adenovirus isolates from sporadic and epidemic ocular infections: experience in a clinical laboratory. | adenovirus isolates from 52 patients with ocular infection over a 3-year period were typed by restriction endonuclease analysis in a clinical laboratory. the results indicated that adenovirus type 8 was the most common cause of adenovirus eye infection during this period, being responsible for 42 (81%) of the 52 cases. of 42 adenovirus type 8 isolates, 22 showed variant patterns by restriction endonuclease analysis and required multiple enzyme digests for identification. these isolates were read ... | 1994 | 8006929 |
| type- and group-specific polymerase chain reaction for adenovirus detection. | we report on a 1,551-base-pair-long dna sequence, encoding the variable region and parts of the flanking conserved regions of the human adenovirus type 8 (av8) hexon, and a sequence comprising 1,404 base pairs, encoding the corresponding regions of the human adenovirus type 31 (av31). comparison of the hexon sequences showed that the major sequence changes were located in loops i1 and i2 of the hexon polypeptides which form the surface of the virion. we established a type-specific polymerase cha ... | 1994 | 8023012 |
| the role of a rapid diagnostic test (adenovirus immune dot-blot) in the control of an outbreak of adenovirus type 8 keratoconjunctivitis. | twenty-three patients were involved in an outbreak of adenovirus type 8 infection based at the manchester royal eye hospital in the spring of 1991. confirmation of adenovirus infection was by means of the immune dot-blot test (idbt) and virus culture, the latter, allowing serotype 8 to be identified as the cause. epidemiological tracing and limited restriction endonuclease analysis of the virus isolated suggest that 15 patients contracted the infection within the casualty department following at ... | 1993 | 8253218 |
| adenovirus type 8 conjunctivitis outbreak in a neonatal intensive care unit. | an outbreak of adenovirus type 8 conjunctivitis occurred in seven premature infants who had undergone ophthalmological examination four to seven days previously. three of the affected infants, treated with steroids because of bronchopulmonary dysplasia, showed systemic manifestations and deterioration of their respiratory disease. second and third waves affected nine staff and 12 family members. | 1993 | 8323370 |
| adenovirus type 8 epidemic keratoconjunctivitis in an eye clinic: risk factors and control. | epidemic keratoconjunctivitis (ekc) due to adenovirus type 8 affected 126 (7%) of 1870 ophthalmology clinic patients during an outbreak. risk factors and mode of transmission were studied by comparing cases (n = 58) and controls (n = 200) for exposure to risk factors. pneumotonometry (odds ratio [or], 10.5; 95% confidence interval [ci], 4.0-27.7), multiple clinic visits (or, 5.9; 95% ci, 3.3-10.6), and contact with an infected physician (or, 3.3; 95% ci, 1.2-9.0) were significant risk factors fo ... | 1993 | 8501319 |
| adenovirus masquerading as microsporidia. | enterocytozoon bieneusi is a microsporidian that causes a severe, debilitating, chronic diarrhea in some patients with the acquired immunodeficiency syndrome. specific diagnosis of e. bieneusi currently requires an invasive biopsy procedure and time-consuming preparation of specimens for electron microscopy. our attempts to establish an in vitro culture system using mammalian cell cultures inoculated with duodenal aspirates, biopsy, or both, from 2 infected patients resulted in inadvertent cocul ... | 1996 | 8604104 |
| two outbreaks of adenovirus type 8 keratoconjunctivitis with different outcome. | in 1993, a case of adenovirus type 8 keratoconjunctivitis acquired in central europe was the starting point of an outbreak in malmö, sweden. of the 33 diagnoses cases, 23 were infected nosocomially. in 29 the diagnosis was verified by virus culture. the outbreak was recognised 15 days after the appearance of the first secondary case. nosocomial transmission was stopped by protective measures which didn't include separation of the infected patients from others. epidemiologic investigation disclos ... | 1995 | 8646586 |
| molecular cloning and restriction enzyme mapping of avian adenovirus type 8 dna. | avian adenovirus (aav) type 8 was cultured in an avian hepatoma cell line designated ch-sah and the viral dna extracted and purified. restriction enzyme analysis of viral dna using the endonucleases apai, ecori, hindiii, kpni, noti, spei, stui and xbai was carried out, and fragments representing the entire genome were cloned. according to the restriction enzyme fragments, the size of the aav type 8 genome was calculated to be 44.7 kb. subcloning of viral dna fragments and hybridization studies u ... | 1996 | 8896244 |
| growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line. | fowl adenoviruses, many of which appear to be non-pathogenic, are ubiquitous in birds. in addition, the genome of these viruses is large, making them ideal candidates for construction as vectors for foreign genes. current methods to cultivate fowl adenoviruses use primary cell cultures derived from embryonated chicken eggs. in order to provide a more suitable culture method, the growth of fowl adenovirus type 8 (fadv-8) was investigated in ch-sah, a continuous hepatoma cell line. a one step grow ... | 1998 | 9763123 |
| sequence and transcriptional analysis of terminal regions of the fowl adenovirus type 8 genome. | fowl adenovirus (fadv) type 1, celo strain has no homologies to mastadenovirus e1a, e1b, e3 and e4, which regulate virus gene expression, dna replication and virus-host interaction. similarly, the right 5 kb and left 15 kb ends of celo virus dna are non-homologous to mastadenoviruses. to compare celo virus with another fadv, 7.5 kb of the left and 17 kb of the right ends of fadv type 8 (strain a-2a) were sequenced and nine and 17 open reading frames (orfs), respectively, were found. this fadv-8 ... | 1998 | 9780058 |
| the survival of adenovirus in multidose bottles of topical fluorescein. | to determine if common ocular adenovirus serotypes survive in vitro in multidose bottles of topical fluorescein (fluress; pilkington barnes hind, inc, sunnyvale, california). | 1998 | 9860013 |
| corneal epithelial changes due to adenovirus type 8 infection. a non-contact photomicrographic in vivo study in the human cornea. | to investigate corneal epithelial changes in human adenovirus type 8 (ad8) infection, and compare them with herpes simplex virus type 1 (hsv1) changes. | 2000 | 10726788 |
| virucidal and chlamydicidal activities of eye drops with povidone-iodine liposome complex. | povidone-iodine (pvp-i) is a broad-spectrum microbicide with in vitro activity against bacteria, viruses, fungi and protozoans. a 5% solution of pvp-i proved to be highly effective in ophthalmic surgery for the prophylaxis of endophthalmitis. for the antiseptic treatment of eye infections a novel application form of pvp-i has been developed by using a pvp-i liposome complex which demonstrated an excellent antimicrobial efficacy. in this study it could be shown that the novel liposomal formulatio ... | 2000 | 10754445 |
| the complete nucleotide sequence of fowl adenovirus type 8. | the fowl adenovirus type 8 (fadv-8) genome was sequenced and found to be 45063 nucleotides in length, the longest adenovirus (adv) genome for which the complete nucleotide sequence has been determined so far. no regions homologous to early regions 1, 3 and 4 (e1, e3 and e4) of mastadenoviruses were recognized. gene homologues for early region 2 (e2) proteins, intermediate protein iva2 and late proteins were found by their similarities to protein sequences from other advs. however, sequences homo ... | 2000 | 10859390 |
| the orf rtl1 transcript of fowl adenovirus type-8 is spliced and truncated at late stages of the virus replication cycle. | two transcription products were found for the open reading frame (orf) rtl1 located near the right terminus of the fowl adenovirus type-8 genome. the larger transcript, which was transcribed mostly during the early stage of the virus infection, contains the complete sequence (933 nucleotides) of the predicted orf from the genomic dna sequence encoding a 311 amino acid (aa) polypeptide. in contrast, the shorter transcript, which was more predominant at the late stage of the infection, was missing ... | 2000 | 10872874 |
| initial interactions of subgenus d adenoviruses with a549 cellular receptors: sialic acid versus alpha(v) integrins. | selected members of the adenovirus family have been shown to interact with the coxsackie adenovirus receptor, alpha(v) integrins, and sialic acid on target cells. initial interactions of subgenus d adenoviruses with target cells have until now been poorly characterized. here, we demonstrate that adenovirus type 8 (ad8), ad19a, and ad37 use sialic acid as a functional cellular receptor, whereas the ad9 and ad19 prototypes do not. | 2000 | 10906228 |
| [bacterial infection in the conjunctiva of patients with adenoviral conjunctivitis]. | we evaluate the microbiological features of mixed infection in adenovirus-infected conjunctiva. | 2000 | 10979295 |
| epidemic keratoconjunctivitis caused by a new genotype of adenovirus type 8 (ad8)-a chronological review of ad8 in southern taiwan. | to understand the pathogenic evolution of viral keratoconjunctivitis in kaohsiung, taiwan, a retrospective molecular and clinical analysis was conducted. | 2001 | 11313048 |
| bacterial infection in the conjunctiva of patients with adenoviral conjunctivitis. | purpose: we evaluate the microbiological features of mixed infection in adenovirus-infected conjunctiva.subjects: isolation of bacteria was performed in 82 samples of adenoviral conjunctivitis at six eye clinics in japan.methods: for microbiological diagnosis, we performed immunochromatography (ic) and polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis for detection and serotyping of adenovirus, and pcr for detection of herpes simplex virus (hsv) and chlamydia ... | 2001 | 11341905 |
| no sequence variation in part of the hexon and the fibre genes of adenovirus 8 isolated from patients with conjunctivitis or epidemic keratoconjunctivitis (ekc) in norway during 1989 to 1996. | several local epidemics of keratoconjunctivitis/conjunctivitis caused by adenovirus type 8 (ad8) occurred in norway from august 1995 to may 1996. a smaller epidemic occurred in 1992. the ad8 hexon forms the surface of the virion and contains the hypervariable regions loop i(1) and loop i(2). the fibre mediates the primary contact with cells. sequence variation in hexon and fibre genes might play an important role in the pathogenicity of adenoviruses. the aim of this study was to investigate the ... | 2001 | 11429431 |
| rapid detection and typing of oculopathogenic strain of subgenus d adenoviruses by fiber-based pcr and restriction enzyme analysis. | to develop a new detection and typing method of oculopathogenic strains of subgenus d adenoviruses directly from conjunctival scrapings by a combination of polymerase chain reaction (pcr) and restriction enzyme analysis (rea). | 2001 | 11481265 |
| virucidal activity and cytotoxicity of the liposomal formulation of povidone-iodine. | two drug formulations of povidone-iodine (pvp-i)--an aqueous pvp-i solution (betaisodona) and a liposomal pvp-i formulation--were tested for their virucidal activity and cytotoxicity in cell culture. as to the virudical activity against influenza a virus, herpes simplex virus type 1, adenovirus type 8 and human rhinovirus type 14, the liposomal formulation of pvp-i proved to be approximately as active as the aqueous one. half maximum cytotoxic pvp-i concentrations were 0.01-0.07% for aqueous pvp ... | 2002 | 12062394 |
| adenovirus type 37 uses sialic acid as a cellular receptor on chang c cells. | epidemic keratoconjunctivitis (ekc) is a severe eye infection caused mainly by adenovirus type 8 (ad8), ad19, and ad37. we have shown that the ekc-causing adenoviruses use sialic acid as a cellular receptor on a549 cells instead of the coxsackie-adenovirus receptor, which is used by most adenoviruses. recently, wu et al. (virology 279:78-89, 2001) proposed that ad37 uses a 50-kda protein as a receptor on chang c conjunctival cells and that this interaction is independent of sialic acid. accordin ... | 2002 | 12163603 |
| genetic characterisation of adenovirus type 8 isolated in hiroshima city over a 15 year period. | to investigate the genetic differences among the strains of adenovirus type 8 (ad8) circulating in hiroshima city, japan, and to study their circulation pattern. | 2003 | 12560390 |
| the change of etiological agents and clinical signs of epidemic viral conjunctivitis over an 18-year period in southern taiwan. | epidemic viral conjunctivitis is a highly contagious eye disease that occurs worldwide and is caused mainly by adenoviruses and enteroviruses. an 18-year analysis of the changes of pathogens and clinical signs in a subtropical and densely populated island presents certain special features. | 2003 | 12768288 |
| an outbreak of epidemic keratoconjunctivtis in a pediatric unit due to adenovirus type 8. | to investigate and control an outbreak of epidemic keratoconjunctivitis (ekc) occurring in a neonatal intensive care unit (nicu) and to determine signs specific for ekc in newborns. | 2003 | 12887240 |
| isolation of adenovirus type 8 (apc type 8) from a case of epidemic keratoconjunctivitis in japan. | | 1957 | 13411136 |
| continuing studies on the association of adenovirus type 8 with epidemic keratoconjunctivitis. | | 1957 | 13469952 |
| some biological characteristics of adenovirus type 8. | | 1958 | 13533402 |
| a laboratory infection with adenovirus type 8; laboratory and epidemiologic observations. | | 1959 | 13626939 |
| association of adenovirus type 8 with epidemic keratoconjunctivitis. | | 1959 | 13649019 |
| attempts to enhance infectivity of adenovirus type 8 for cell cultures. | | 1962 | 13904376 |
| [studies of the immunity to epidemic keratoconjunctivitis. i. effectiveness of high titer vaccine with adenovirus type 8]. | | 1961 | 13919490 |
| [laboratory infection with adenovirus type 8]. | | 1963 | 13937654 |
| adenovirus type 8 and the story of epidemic keratoconjunctivitis. | | 1962 | 13957563 |
| infections due to adenovirus type 8 in the united states. ii. community-wide infection with adenovirus type 8. | | 1963 | 14025591 |
| infections due to adenovirus type 8 in the united states. i. an outbreak of epidemic keratoconjunctivitis originating in a physician's office. | | 1963 | 14025592 |
| accidental laboratory infections by adenovirus type 8. | | 1963 | 14043496 |
| aetiologic relationship of adenovirus type 8 to the epidemic of keratoconjunctivitis in szeged. | | 1963 | 14043497 |
| [studies on an epidemic of keratoconjunctivitis caused by adenovirus type 8. i. isolation of the virus in primary human amnion tissue culture with the results of serological studies]. | | 1963 | 14055696 |
| [studies on an epidemic of keratoconjunctivitis caused by adenovirus type 8. ii. clinical and epidemiological studies]. | | 1963 | 14055697 |
| some biological characteristics of adenovirus type 8 strains. | | 1963 | 14160951 |