| cloning and characterization of the c1 repressor of pseudomonas aeruginosa bacteriophage d3: a functional analog of phage lambda ci protein. | we cloned the gene (c1) which encodes the repressor of vegetative function of pseudomonas aeruginosa bacteriophage d3. the cloned gene was shown to inhibit plating of d3 and the induction of d3 lysogens by uv irradiation. the efficiency of plating and prophage induction of the heteroimmune p. aeruginosa phage f116l were not affected by the presence of the cloned c1 gene of d3. when the d3 dna fragment containing c1 was subcloned into pbr322 and introduced into escherichia coli, it was shown to s ... | 1987 | 3106321 |
| [temperature-sensitive mutant of bacteriophage d3 and its use for the purpose of demonstrating the location of prophage in pseudomonas aeruginosa cells]. | for the first time a thermoinducible mutant, known as d3ct, has been obtained from pseudomonas aeruginosa phage and used for lysogenizing culture pao2604 met--ilv--. after a temperature shock phage d3ctwas induced from lysogenic strain pao2604 (d3ct), and its development went along the lytic line. from the population of pao2604 (d3ct) cells only 0.07% survived; in these cells the faulty excision of the prophage probably occurred at a high temperature. after thermoinduction 2.8% of the colonies f ... | 1980 | 6778028 |
| specialized transduction of pseudomonas aeruginosa pao by bacteriophage d3. | d3, a temperate bacteriophage of pseudomonas aeruginosa pao, was found to specifically transduce the alleles met-49 and met-117. induction of established lysogens with uv light was necessary for the production of transducing lysates. transduced cells were immune to superinfection by phage d3 and could give rise to high-frequency transducing lysates. cotransduction of these two alleles could not be demonstrated. met-117 was mapped to 26 min on the pao genetic map. complementation studies using th ... | 1986 | 3080405 |
| is222, a new insertion element associated with the genome of pseudomonas aeruginosa. | a new insertion element, is222, was identified to be associated with the dna of a mutant strain of the converting pseudomonas aeruginosa bacteriophage d3. the insertion sequence was 1,350 base pairs in size and possessed terminal inverted repeats. the nucleotide sequence contained single cleavage sites for ecori and pvui but none for bamhi, psti, hindiii, smai, or sali. by southern hybridization analysis, no homology was found with genomic dna from p. aeruginosa pat or escherichia coli. genomic ... | 1986 | 3011736 |
| construction of broad-host-range vectors for the selection of divergent promoters. | a series of promoter-probe plasmid vectors has been constructed which allows for the selection of dna sequences containing divergent control elements. each vector contains a pair of promoterless genes [encoding beta-galactosidase (lacz), alkaline phosphatase (phoa), and bacterial luciferase (luxab)] arranged in an antiparallel fashion and separated by a large intervening multiple cloning site. the vectors permit direct detection of promoter activity on indicator plates after transformation. clon ... | 1990 | 2199327 |
| transduction of a plasmid containing the bacteriophage d3 cos site in pseudomonas aeruginosa. | plasmids harboring the cos sequences of bacteriophage d3 can be transferred, by bacteriophage d3, into pseudomonas aeruginosa by a mechanism which is insensitive to dnase. transducing activity was separated from the plaque-forming particles by cscl equilibrium gradient centrifugation. restriction endonuclease digestion patterns suggest that the transducing particles contain plasmid concatemers. | 1990 | 2111812 |
| characteristics of a novel pseudomonas aeruginosa bacteriophage, paju2, which is genetically related to bacteriophage d3. | pseudomonas aeruginosa bacteriophage (phage) is one of the most taxonomically and genetically diverse phages. although phage d3 is one of well-studied p. aeruginosa phages, no d3-related p. aeruginosa phage has been reported. we report a novel p. aeruginosa siphovirus, paju2, which is genetically related to but morphology distinct (highly elongated head) from phage d3. a paju2 capsid protein, orf3, is thought to be synthesized as a protein fused to a prohead protease and is autocatalytically cle ... | 2009 | 19010363 |
| three-component-mediated serotype conversion in pseudomonas aeruginosa by bacteriophage d3. | bacteriophage d3 is capable of lysogenizing pseudomonas aeruginosa pao1 (serotype o5), converting the o-antigen from o5 to o16 and o-acetylating the n-acetylfucosamine moiety. to investigate the mechanism of lysogenic conversion, a 3.6 kb fragment from the d3 genome was isolated capable of mediating serotypic conversion identical to the d3 lysogen strain (ak1380). the pao1 transformants containing this 3.6 kb of d3 dna exhibited identical lipopolysaccharide (lps) banding patterns to serotype o16 ... | 2001 | 11251840 |
| sequence of the genome of the temperate, serotype-converting, pseudomonas aeruginosa bacteriophage d3. | temperate bacteriophage d3, a member of the virus family siphoviridae, is responsible for serotype conversion in its host, pseudomonas aeruginosa. the complete sequence of the double-stranded dna genome has been determined. the 56,426 bp contains 90 putative open reading frames (orfs) and four genes specifying trnas. the latter are specific for methionine (aug), glycine (gga), asparagine (aac), and threonine (aca). the trnas may function in the translation of certain highly expressed proteins fr ... | 2000 | 11029426 |
| cloning and analysis of the capsid morphogenesis genes of pseudomonas aeruginosa bacteriophage d3: another example of protein chain mail? | the terminal dna restriction fragments (psti-d and -b) of pseudomonas aeruginosa bacteriophage d3 were ligated, cloned, and sequenced. of the nine open reading frames in this 8.3-kb fragment, four were identified as encoding large-subunit terminase, portal, clpp protease, and major head proteins. the portal and capsid proteins showed significant homology with proteins of the lambdoid coliphage hk97. phage d3 was purified by cscl equilibrium gradient centrifugation (rho = 1.533 g/ml), and sodium ... | 1999 | 10572124 |
| transfer rna genes and their significance to codon usage in the pseudomonas aeruginosa lamboid bacteriophage d3. | using trnascan-se and fastrna we have identified four trna genes in the delayed early region of the bacteriophage d3 genome (genbank accession no. af077308). these are specific for methionine (aug), glycine (gga), asparagine (aac), and threonine (aca). the d3 thr- and gly-trnas recognize codons, which are rarely used in pseudomonas aeruginosa and presumably, influence the rate of translation of phage proteins. blastn searches revealed that the d3 trna genes have homology to trna genes from gram- ... | 1999 | 10526403 |
| overexpression, purification, and analysis of the c1 repressor protein of pseudomonas aeruginosa bacteriophage d3. | a 3.1-kb region of the bacteriophage d3 genome which contains the immunity functions has recently been sequenced (genbank accession no. l22692). sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophage in the chromosome of pseudomonas aeruginosa. a plasmid was constructed that overexpresses repressor c1 protein under control of p(tac) in escherichia coli. c1 protein was subseque ... | 1997 | 9090110 |
| genetic and sequence analysis of the cos region of the temperate pseudomonas aeruginosa bacteriophage, d3. | the location and structure of the cos ends of bacteriophage d3, which infects pseudomonas aeruginosa strain pao, has been determined using a combination of deletion analysis, transposon mutagenesis, and sequencing directly off the phage dna. phage d3 was found to have 9-bp 3' cos ends, making it the first phage of a gram-negative organism known to have 3' cos ends. a 700-bp region flanking the cos site was necessary for efficient transduction of d3 cosmid derivatives. this region was found to co ... | 1996 | 8921844 |
| cloning of the early promoters of pseudomonas aeruginosa bacteriophage d3: sequence of the immunity region of d3. | the early promoters of bacteriophage d3 of pseudomonas aeruginosa were cloned and physically mapped to the right 25% of the phage genome. the promoters were cloned into promoter selection vector pqf26, and their relative strengths, the direction of transcription, and whether they were directly regulated by repressor were determined. a 3.3-kb fragment of the genome containing the immunity region was sequenced and analyzed (genbank accession number: l22692). the promoter activity associated with t ... | 1994 | 8050993 |
| o-antigen conversion in pseudomonas aeruginosa pao1 by bacteriophage d3. | the lysogenization of pseudomonas aeruginosa pao by phage d3 results in derivatives which are resistant to superinfection by phage d3c by virtue of the fact that homologous phage cannot adsorb to these cells. the serologically and morphologically unrelated phage e79 showed a markedly decreased adsorption rate to the lysogen pao(d3). since both of these phages are lipopolysaccharide specific, these results suggested lysogenic conversion of the phage receptor. the lipopolysaccharide was extracted ... | 1983 | 6190794 |
| serotype-converting bacteriophage d3 of pseudomonas aeruginosa: vegetative and prophage restriction maps. | d3 is a temperate serotype-converting bacteriophage of pseudomonas aeruginosa. a restriction map, based upon bamhi, psti, pvui, hindiii and smai sites, indicates that the phage genome is 56.4 kb long, and that it possesses cohesive ends. the prophage map suggests a unique insertion site in the strain ak1380 genome. phage dna integration occurs upon the circularization of d3 genome with the integration point approximately equidistant from the two ends. | 1987 | 3036658 |